MD06G1220800.v1.1

conserved protein UCP031088, alpha beta hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010669
Physical Location & Seq
Reverse (-)
35257489 .. 35261079
3591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1220800.v1.1.491

Sequence Viewer

Length: 1422 bp
ATGTCGTTTCTCGCTCCGCATTCGGATCTTCGTTCCCTGACGGCGAATCGTGGTACTGACCGTTGGTGTTTCAGCCAAATCACATGGCCCGACCCGAAACCCCAGCTGTTCCGGGTCTCTGCTCGTCAGAGTCCGGCGGCAAATGGCATCGCTTTGGATTTGAAGAAGAGAAAGGACCCGCGGTGGTCGGTTTGTACCGCCGACGAGCTTCACTATGTCTCTGTTCCAAAATCCGATTGGAGCCTCGCTCTCTGGCGCTACCTTCCCTCTCCTCAGGCGGAGCCAAGGAATCATCCGCTGCTGCTGCTGTCAGGACTTGCGACGAATGCGATTGGATACGATCTTTCTCCCGAGTCCTCGTTTGCGCGCTCCATGTCTGCTGAAGGATATGATACATGGATTCTTGAGATGAGAGGTGCAGGGTTGAGCACATATGGTATGGACTTAGGGGCAGTGAAGCGGCCTCCCCATGCCATGTCCAAGCAGACAATTTCTTCGAATGGGACATATAATTTGGTGTCTCAATCGGATGAATTGCAACTAGTGAGAAACTTTATGGAGACCTTTATGCAATTATCTGAAAGATTATCAAGGTTTGCAGACTTCCAAGAACGTTTTTCTACTGCCTTAGAAGATTTCCAGAAACAGCTCGACTTAATTGTAAAGTGTGACTGGGACTTTGATCACTACTTGGAAGAGGATGTGCCTGTTGCGATGGAGTATATAAGAACTCAATGCAAACCAAGGGATGGCAAGCTCCTTGCTGTTGGTCACTCGATGGGCGGTATCTTGCTATATGCAATGCTCTCACGCTGCTCTTACCAAGGGCGAGATTCTGGGTTGGCATCAGTTGCTACTTTGGCATCATCGCTCGACTACACGCAGTCAAGATCTTCGTGCAAGTTATTTTTACCTCTGGCAGACCCAGCACAGGTTCTAAACGTTCCTGCCGTTCCACTTGGTCTATTTGCCACTGCTGTGCATAATCTTGGTACTCGCACTCCAATTTCGTCTTGGCTTAAGCTTCAAGTTTCTGCTCAAGGCATGATGCAGCCAGATTTGTTCGAAAAGCTTGTTTTAAACAACTTTTGTACCGTGCCCGCTAAGGTTCTCTTGCAGCTATCAACACTTTTTGCGGATGGGGGTTTATGTGACAGGAGTGGAACTTTCTTTTACAAGGATCACCTTTGCAAAAGCAATGTCCCAATCTTAGCTCTTGGAGGCGATCAAGACCTAATATGTCCTCTCGAAGCTGTTTATGAAACAGTGAAGCTGATTCCCGAAGAAATGGTTACTTTCAAAGTTCTCGGAGAAGCCAGCGGTCCTCATTTTGCTCACTATGATTTAGTAGGAGGTCGCTTGGCAGCAGATACAGTATATCCGCACATTTTCAAATTTCTCAATCGTCATGACAGGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

474

Amino Acids

52.73

Weight (kDa)

6.32

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 99 - 433 1.3e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010669)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73750 AT1G73750
fragaria_vesca FvH4_5g16710 FvH4_5g16710 FvH4_5g16710
malus_domestica MD06G1220800.v1.1
prunus_persica Prupe.5G225200_v2.0.a1 Prupe.5G225200_v2.0.a1
pyrus_communis pycom06g19670
rosa_chinensis RchiOBHm_Chr7g0179391
rosa_laevigata RLG00000005360
rosa_multiflora Rmu_sc0002581.1_g000004 Rmu_sc0019996.1_g000001
rosa_roxburghii Rroxscaffold_3G00274070
rosa_rugosa Rorug06G0424000
rosa_samantha Rh7AG023400 Rh7BG023000 Rh7CG024400 Rh7DG023900
rosa_wichuraiana Rw7G001890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 749
AccII CGCG 2 cut(s) 181, 367
AclI AACGTT 2 cut(s) 613, 942
AclWI GGATC 2 cut(s) 33, 1188
AcsI RAATTY 1 cut(s) 1394
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 4 cut(s) 55, 196, 994, 1093
AfiI CCNNNNNNNGG 2 cut(s) 749, 931
AflII CTTAAG 1 cut(s) 1019
AgsI TTSAA 4 cut(s) 163, 1028, 1300, 1393
AhlI ACTAGT 1 cut(s) 541
AleI CACNNNNGTG 1 cut(s) 977
Alw21I GWGCWC 1 cut(s) 431
Alw26I GTCTC 4 cut(s) 121, 223, 525, 554
AlwI GGATC 2 cut(s) 33, 1188
AlwNI CAGNNNCTG 1 cut(s) 1419
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 2 cut(s) 86, 461
ApeKI GCWGC 7 cut(s) 298, 301, 304, 813, 1051, 1117, 1364
ApoI RAATTY 1 cut(s) 1394
ArsI GACNNNNNNTTYG 8 cut(s) 478, 496, 510, 528, 662, 694, 1185, 1217
AspLEI GCGC 3 cut(s) 258, 367, 369
AspS9I GGNCC 4 cut(s) 87, 175, 1322, 1416
AsuC2I CCSGG 1 cut(s) 113
AsuHPI GGTGA 1 cut(s) 1175
AsuII TTCGAA 2 cut(s) 497, 1065
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 3 cut(s) 175, 1322, 1416
AxyI CCTNAGG 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 1101
BaeI ACNNNNGTAYC 2 cut(s) 1075, 1108
Bbv12I GWGCWC 1 cut(s) 431
BbvI GCAGC 7 cut(s) 285, 288, 291, 800, 1063, 1129, 1376
BccI CCATC 4 cut(s) 709, 743, 772, 1133
BceAI ACGGC 2 cut(s) 57, 935
BciVI GTATCC 1 cut(s) 329
BclI TGATCA 1 cut(s) 682
BcnI CCSGG 1 cut(s) 113
BcoDI GTCTC 4 cut(s) 121, 223, 525, 554
BcuI ACTAGT 1 cut(s) 541
BfaI CTAG 1 cut(s) 542
BfoI RGCGCY 1 cut(s) 259
BfrI CTTAAG 1 cut(s) 1019
BfuI GTATCC 1 cut(s) 329
BglII AGATCT 1 cut(s) 890
BisI GCNGC 9 cut(s) 138, 299, 302, 305, 461, 814, 1052, 1118, 1365
BlsI GCNGC 9 cut(s) 139, 300, 303, 306, 462, 815, 1053, 1119, 1366
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 3 cut(s) 175, 1322, 1416
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 4 cut(s) 87, 175, 1322, 1416
BmiI GGNNCC 3 cut(s) 177, 242, 282
BmrFI CCNGG 1 cut(s) 113
BmrI ACTGGG 1 cut(s) 682
BmsI GCATC 4 cut(s) 156, 854, 872, 1038
BmuI ACTGGG 1 cut(s) 682
BplI GAGNNNNNCTC 2 cut(s) 232, 264
Bpu10I CCTNAGC 1 cut(s) 1104
Bpu14I TTCGAA 2 cut(s) 497, 1065
BpuEI CTTGAG 2 cut(s) 425, 1023
BpuMI CCSGG 1 cut(s) 113
BsaI GGTCTC 2 cut(s) 121, 554
BsaJI CCNNGG 4 cut(s) 179, 284, 743, 823
BsaXI ACNNNNNCTCC 2 cut(s) 985, 1015
Bsc4I CCNNNNNNNGG 2 cut(s) 749, 931
Bse1I ACTGG 1 cut(s) 677
Bse21I CCTNAGG 1 cut(s) 273
Bse3DI GCAATG 2 cut(s) 807, 1204
BseDI CCNNGG 4 cut(s) 179, 284, 743, 823
BseGI GGATG 5 cut(s) 292, 535, 706, 754, 1144
BseLI CCNNNNNNNGG 2 cut(s) 749, 931
BseMI GCAATG 2 cut(s) 807, 1204
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 1 cut(s) 677
BsePI GCGCGC 1 cut(s) 365
BseRI GAGGAG 1 cut(s) 261
BseSI GKGCMC 1 cut(s) 1101
BseXI GCAGC 7 cut(s) 285, 288, 291, 800, 1063, 1129, 1376
BseYI CCCAGC 2 cut(s) 102, 925
BsgI GTGCAG 1 cut(s) 438
Bsh1236I CGCG 2 cut(s) 181, 367
BshFI GGCC 2 cut(s) 88, 463
BsiHKAI GWGCWC 1 cut(s) 431
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 2 cut(s) 112, 134
BslFI GGGAC 3 cut(s) 517, 689, 1187
BslI CCNNNNNNNGG 2 cut(s) 749, 931
BsmAI GTCTC 4 cut(s) 121, 223, 525, 554
BsmFI GGGAC 3 cut(s) 517, 689, 1187
BsmI GAATGC 2 cut(s) 19, 331
BsnI GGCC 2 cut(s) 88, 463
Bso31I GGTCTC 2 cut(s) 121, 554
BsoBI CYCGRG 1 cut(s) 350
Bsp119I TTCGAA 2 cut(s) 497, 1065
Bsp1286I GDGCHC 2 cut(s) 431, 1101
Bsp143I GATC 6 cut(s) 25, 340, 682, 890, 1180, 1225
BspANI GGCC 2 cut(s) 88, 463
BspCNI CTCAG 1 cut(s) 286
BspFNI CGCG 2 cut(s) 181, 367
BspHI TCATGA 1 cut(s) 1408
BspLI GGNNCC 3 cut(s) 177, 242, 282
BspPI GGATC 2 cut(s) 33, 1188
BspT104I TTCGAA 2 cut(s) 497, 1065
BspTI CTTAAG 1 cut(s) 1019
BspTNI GGTCTC 2 cut(s) 121, 554
BsrDI GCAATG 2 cut(s) 807, 1204
BsrI ACTGG 1 cut(s) 677
BssECI CCNNGG 4 cut(s) 179, 284, 743, 823
BssHII GCGCGC 1 cut(s) 365
BssMI GATC 6 cut(s) 25, 340, 682, 890, 1180, 1225
BssT1I CCWWGG 3 cut(s) 284, 743, 823
Bst4CI ACNGT 4 cut(s) 62, 1096, 1267, 1375
Bst6I CTCTTC 2 cut(s) 161, 690
BstAFI CTTAAG 1 cut(s) 1019
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBI TTCGAA 2 cut(s) 497, 1065
BstC8I GCNNGC 4 cut(s) 367, 755, 1101, 1318
BstDEI CTNAG 5 cut(s) 273, 445, 628, 1104, 1210
BstDSI CCRYGG 1 cut(s) 179
BstF5I GGATG 5 cut(s) 292, 535, 706, 754, 1144
BstFNI CGCG 2 cut(s) 181, 367
BstH2I RGCGCY 1 cut(s) 259
BstHHI GCGC 3 cut(s) 258, 367, 369
BstKTI GATC 6 cut(s) 28, 343, 685, 893, 1183, 1228
BstMAI GTCTC 4 cut(s) 121, 223, 525, 554
BstMBI GATC 6 cut(s) 25, 340, 682, 890, 1180, 1225
BstMWI GCNNNNNNNGC 5 cut(s) 304, 326, 851, 860, 926
BstSCI CCNGG 1 cut(s) 111
BstSLI GKGCMC 1 cut(s) 1101
BstUI CGCG 2 cut(s) 181, 367
BstV1I GCAGC 7 cut(s) 285, 288, 291, 800, 1063, 1129, 1376
BstX2I RGATCY 2 cut(s) 25, 890
BstYI RGATCY 2 cut(s) 25, 890
Bsu36I CCTNAGG 1 cut(s) 273
BsuI GTATCC 1 cut(s) 329
BsuRI GGCC 2 cut(s) 88, 463
BtgI CCRYGG 1 cut(s) 179
BtgZI GCGATG 3 cut(s) 133, 728, 852
BtsCI GGATG 5 cut(s) 292, 535, 706, 754, 1144
BtsI GCAGTG 2 cut(s) 459, 972
BtsIMutI CAGTG 3 cut(s) 459, 972, 1272
Cac8I GCNNGC 4 cut(s) 367, 755, 1101, 1318
CaiI CAGNNNCTG 1 cut(s) 1419
CciI TCATGA 1 cut(s) 1408
CfoI GCGC 3 cut(s) 258, 367, 369
Cfr13I GGNCC 4 cut(s) 87, 175, 1322, 1416
Cfr42I CCGCGG 1 cut(s) 182
Csp6I GTAC 4 cut(s) 54, 195, 993, 1092
CviAII CATG 7 cut(s) 84, 373, 396, 470, 475, 1045, 1409
CviQI GTAC 4 cut(s) 54, 195, 993, 1092
DdeI CTNAG 5 cut(s) 273, 445, 628, 1104, 1210
DpnI GATC 6 cut(s) 27, 342, 684, 892, 1182, 1227
DpnII GATC 6 cut(s) 25, 340, 682, 890, 1180, 1225
DraI TTTAAA 1 cut(s) 1080
Eam1104I CTCTTC 2 cut(s) 161, 690
EarI CTCTTC 2 cut(s) 161, 690
EciI GGCGGA 1 cut(s) 293
Eco130I CCWWGG 3 cut(s) 284, 743, 823
Eco31I GGTCTC 2 cut(s) 121, 554
Eco47I GGWCC 3 cut(s) 175, 1322, 1416
Eco57I CTGAAG 1 cut(s) 402
Eco81I CCTNAGG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 350
EcoO109I RGGNCCY 2 cut(s) 175, 1416
EcoT14I CCWWGG 3 cut(s) 284, 743, 823
ErhI CCWWGG 3 cut(s) 284, 743, 823
FaeI CATG 7 cut(s) 87, 376, 399, 473, 478, 1048, 1412
FalI AAGNNNNNCTT 2 cut(s) 1097, 1129
FaqI GGGAC 3 cut(s) 517, 689, 1187
FatI CATG 7 cut(s) 83, 372, 395, 469, 474, 1044, 1408
FauI CCCGC 2 cut(s) 186, 1108
FauNDI CATATG 1 cut(s) 433
FbaI TGATCA 1 cut(s) 682
Fnu4HI GCNGC 9 cut(s) 138, 299, 302, 305, 461, 814, 1052, 1118, 1365
FokI GGATG 5 cut(s) 279, 542, 713, 761, 1151
Fsp4HI GCNGC 9 cut(s) 138, 299, 302, 305, 461, 814, 1052, 1118, 1365
FspBI CTAG 1 cut(s) 542
GlaI GCGC 3 cut(s) 257, 366, 368
GluI GCNGC 9 cut(s) 138, 299, 302, 305, 461, 814, 1052, 1118, 1365
GsaI CCCAGC 2 cut(s) 106, 929
HaeII RGCGCY 1 cut(s) 259
HaeIII GGCC 2 cut(s) 88, 463
HapII CCGG 2 cut(s) 112, 134
HhaI GCGC 3 cut(s) 258, 367, 369
Hin1II CATG 7 cut(s) 87, 376, 399, 473, 478, 1048, 1412
Hin6I GCGC 3 cut(s) 256, 365, 367
HinP1I GCGC 3 cut(s) 256, 365, 367
HindIII AAGCTT 2 cut(s) 1022, 1070
HinfI GANTC 7 cut(s) 46, 130, 289, 353, 400, 833, 1276
HpaII CCGG 2 cut(s) 112, 134
HphI GGTGA 1 cut(s) 1175
Hpy188I TCNGA 6 cut(s) 25, 129, 235, 529, 580, 1310
Hpy188III TCNNGA 9 cut(s) 312, 350, 404, 640, 888, 1229, 1247, 1280, 1409
Hpy99I CGWCG 2 cut(s) 206, 325
HpyAV CCTTC 2 cut(s) 272, 377
HpyCH4III ACNGT 4 cut(s) 62, 1096, 1267, 1375
HpyCH4IV ACGT 2 cut(s) 613, 942
HpyF10VI GCNNNNNNNGC 5 cut(s) 304, 326, 851, 860, 926
HpyF3I CTNAG 5 cut(s) 273, 445, 628, 1104, 1210
HpySE526I ACGT 2 cut(s) 613, 942
Hsp92II CATG 7 cut(s) 87, 376, 399, 473, 478, 1048, 1412
HspAI GCGC 3 cut(s) 256, 365, 367
Ksp22I TGATCA 1 cut(s) 682
KspI CCGCGG 1 cut(s) 182
Kzo9I GATC 6 cut(s) 25, 340, 682, 890, 1180, 1225
LmnI GCTCC 5 cut(s) 19, 240, 280, 374, 762
Lsp1109I GCAGC 7 cut(s) 285, 288, 291, 800, 1063, 1129, 1376
LweI GCATC 4 cut(s) 156, 854, 872, 1038
MaeI CTAG 1 cut(s) 542
MaeII ACGT 2 cut(s) 613, 942
MaeIII GTNAC 4 cut(s) 668, 770, 1151, 1291
MalI GATC 6 cut(s) 27, 342, 684, 892, 1182, 1227
MboI GATC 6 cut(s) 25, 340, 682, 890, 1180, 1225
MboII GAAGA 8 cut(s) 20, 175, 178, 486, 644, 707, 885, 1295
MflI RGATCY 2 cut(s) 25, 890
MhlI GDGCHC 2 cut(s) 431, 1101
MluCI AATT 7 cut(s) 489, 511, 533, 572, 657, 1005, 1394
MlyI GAGTC 2 cut(s) 139, 362
MseI TTAA 3 cut(s) 656, 1020, 1079
MslI CAYNNNNRTG 1 cut(s) 977
MspA1I CMGCKG 4 cut(s) 106, 181, 298, 1320
MspCI CTTAAG 1 cut(s) 1019
MspI CCGG 2 cut(s) 112, 134
MspR9I CCNGG 1 cut(s) 113
Mva1269I GAATGC 2 cut(s) 19, 331
MvnI CGCG 2 cut(s) 181, 367
MwoI GCNNNNNNNGC 5 cut(s) 304, 326, 851, 860, 926
NciI CCSGG 1 cut(s) 113
NdeI CATATG 1 cut(s) 433
NdeII GATC 6 cut(s) 25, 340, 682, 890, 1180, 1225
NlaIII CATG 7 cut(s) 87, 376, 399, 473, 478, 1048, 1412
NlaIV GGNNCC 3 cut(s) 177, 242, 282
NmuCI GTSAC 3 cut(s) 668, 770, 1151
NspV TTCGAA 2 cut(s) 497, 1065
OliI CACNNNNGTG 1 cut(s) 977
PagI TCATGA 1 cut(s) 1408
PauI GCGCGC 1 cut(s) 365
PcsI WCGNNNNNNNCGW 1 cut(s) 948
PctI GAATGC 2 cut(s) 19, 331
PfeI GAWTC 5 cut(s) 46, 289, 400, 833, 1276
PflMI CCANNNNNTGG 1 cut(s) 749
PkrI GCNGC 9 cut(s) 139, 300, 303, 306, 462, 815, 1053, 1119, 1366
PleI GAGTC 2 cut(s) 138, 361
PpsI GAGTC 2 cut(s) 138, 361
PpuMI RGGWCCY 2 cut(s) 175, 1416
Psp1406I AACGTT 2 cut(s) 613, 942
Psp5II RGGWCCY 2 cut(s) 175, 1416
PspFI CCCAGC 2 cut(s) 102, 925
PspN4I GGNNCC 3 cut(s) 177, 242, 282
PspPI GGNCC 4 cut(s) 87, 175, 1322, 1416
PspPPI RGGWCCY 2 cut(s) 175, 1416
PstNI CAGNNNCTG 1 cut(s) 1419
PsuI RGATCY 2 cut(s) 25, 890
PteI GCGCGC 1 cut(s) 365
PvuII CAGCTG 1 cut(s) 106
RsaI GTAC 4 cut(s) 55, 196, 994, 1093
RsaNI GTAC 4 cut(s) 54, 195, 993, 1092
RseI CAYNNNNRTG 1 cut(s) 977
SacII CCGCGG 1 cut(s) 182
SaqAI TTAA 3 cut(s) 656, 1020, 1079
SatI GCNGC 9 cut(s) 138, 299, 302, 305, 461, 814, 1052, 1118, 1365
Sau3AI GATC 6 cut(s) 25, 340, 682, 890, 1180, 1225
Sau96I GGNCC 4 cut(s) 87, 175, 1322, 1416
SchI GAGTC 2 cut(s) 139, 362
ScrFI CCNGG 1 cut(s) 113
SduI GDGCHC 2 cut(s) 431, 1101
SfaNI GCATC 4 cut(s) 156, 854, 872, 1038
Sfr303I CCGCGG 1 cut(s) 182
SfuI TTCGAA 2 cut(s) 497, 1065
SgrBI CCGCGG 1 cut(s) 182
SinI GGWCC 3 cut(s) 175, 1322, 1416
SmiMI CAYNNNNRTG 1 cut(s) 977
SmlI CTYRAG 3 cut(s) 404, 1019, 1038
SmoI CTYRAG 3 cut(s) 404, 1019, 1038
SpeI ACTAGT 1 cut(s) 541
Sse9I AATT 7 cut(s) 489, 511, 533, 572, 657, 1005, 1394
SspMI CTAG 1 cut(s) 542
StyD4I CCNGG 1 cut(s) 111
StyI CCWWGG 3 cut(s) 284, 743, 823
TaaI ACNGT 4 cut(s) 62, 1096, 1267, 1375
TaiI ACGT 2 cut(s) 616, 945
TaqI TCGA 6 cut(s) 497, 651, 776, 873, 1065, 1248
TasI AATT 7 cut(s) 489, 511, 533, 572, 657, 1005, 1394
TauI GCSGC 2 cut(s) 140, 463
TfiI GAWTC 5 cut(s) 46, 289, 400, 833, 1276
Tru1I TTAA 3 cut(s) 656, 1020, 1079
Tru9I TTAA 3 cut(s) 656, 1020, 1079
TscAI CASTG 3 cut(s) 459, 979, 1272
TseFI GTSAC 3 cut(s) 668, 770, 1151
TseI GCWGC 7 cut(s) 298, 301, 304, 813, 1051, 1117, 1364
Tsp45I GTSAC 3 cut(s) 668, 770, 1151
TspDTI ATGAA 2 cut(s) 546, 1275
TspRI CASTG 3 cut(s) 459, 979, 1272
Van91I CCANNNNNTGG 1 cut(s) 749
Vha464I CTTAAG 1 cut(s) 1019
VpaK11BI GGWCC 3 cut(s) 175, 1322, 1416
XapI RAATTY 1 cut(s) 1394
XcmI CCANNNNNNNNNTGG 2 cut(s) 234, 1011
XspI CTAG 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.