pycom06g19670

conserved protein UCP031088, alpha beta hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0010669
Physical Location & Seq
Reverse (-)
23008303 .. 23011321
3019 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g19670.2

Sequence Viewer

Length: 1578 bp
ATGAGTTATGCGCTTTATTGTCCGGCGGGTCATGCCCTCCTCCCAAATAACGGAATTAACCAAATATTACACGCTTTCTCTCCTCCCTCCCGGGAAACAACCATTTCTTCTCCTCCAATAAAACGGCAGCCAAACACCACCAGAAAGAAAAACAACATGTCGCTTCTCGCTCCGCATTCGGATCTTCGTTCCCTGACCGCGATTCGTGGTACGGACCGTTGGAGTTTCGGCCAAATCACATGGCCCGACCCGAAACCCGAGCCGTTCCGGGTCTTTGCCCGTCAGAGTCCGGTGGCAAACGGCGTCGCTTTGGATTTGAAGAAAAGAAAGGACCCGCGGTGGTCGGTCTGTACCGCCGACGAGCTTCACTATGTCTCTGTTCCAAAATCCGATTGGAGCCTCGCTCTCTGGCGCTACCTTCCCTCTCCTCAGGCGGAGCCGAGGAATCATCCGTTGCTGCTGCTGTCAGGACTTGCGACGAATGCGATTGGATACGATCTTTCTCCCGAGTCCTCGTTTGCACGCTCCATGTCTGCTGAAGGATATGATACATGGATTCTTGAGATGAGAGGTGCAGGGTTGAGCACATATGGTATGGACTTAGGGGCGGTGAAGCGGCCTCCCCATGCCATGTCCAAGCAGACAATTTCTTCGAATGGGACATATAATTTGGTGTCTCAATCGGATGAATTGCAACTAGTGAGAAACTTTATGGAGACCTTTATGCAATTATCTGAAAGATTATCAAGGTTTGCAGACTTCCAAGAACGTTTTTCTACTGCCTTAGAAGATTTCCAGAAACAGCTCAACTTAATTGTAAAGTGTGACTGGGACTTTGATCACTACTTGGAAGAGGATGTGCCTGTTGCGATGGAGTATATAAGAACTCAATGCAAACCAAGGGATGGCAAGCTTCTTGCTGTTGGTCACTCGATGGGCGGTATCTTGCTATATGCAATGCTCTCACGCTGCTCTTACCAAGGGCGAGATTCTGGGTTGGCATCAGTTGCTACTTTGGCATCATCGCTTGACTACACGCAGTCAAGATCTTCGTGCAAGTTATTTTTACCTCTGGCTGACCCAGCACAGGTTCTAAACGTTCCTGCCGTTCCATTTGATCTAATTGCCACTGCTGTGCATCATCTTGGTACTCGCACTCCAATTTCGTCTTGGCTTAAGCCTCAAGTTTCTGCTCAAGGCATGATGCAGCCAGATTTGTTCAGAAAGCTTATTTTAAACAACTTTTGTACCGTGCCCGCTAAGCTTCTCTTGCAGCTAGCAACACTTTTCGCGGATGGGGGTTTATGTGACAGGAGTGGAACTTTCTTTTACAAGGATCACCTTTGCAAAAGCAATGTCCCAATCTTAGCTCTTGGAGGCGATCAAGACCTAATATGTCCTCTCGAAGCTGTTTACGAAACAGTGAAGCTGATTCCCGAAGAAATGGTTACTTTCAAAGTTCTTGGAGAAGCCAGCGGTCCTCATTATGCTCACTACGATTTAGTAGGAGGTCGCTTGGCAGCAGATACAGTATATCCGCACATTTTCAAATTTCTCAATCGTCATGACAGGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

526

Amino Acids

58.56

Weight (kDa)

7.98

Isoelectric Point (pI)

53.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010669)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73750 AT1G73750
fragaria_vesca FvH4_5g16710 FvH4_5g16710 FvH4_5g16710
malus_domestica MD06G1220800.v1.1
prunus_persica Prupe.5G225200_v2.0.a1 Prupe.5G225200_v2.0.a1
pyrus_communis pycom06g19670
rosa_chinensis RchiOBHm_Chr7g0179391
rosa_laevigata RLG00000005360
rosa_multiflora Rmu_sc0002581.1_g000004 Rmu_sc0019996.1_g000001
rosa_roxburghii Rroxscaffold_3G00274070
rosa_rugosa Rorug06G0424000
rosa_samantha Rh7AG023400 Rh7BG023000 Rh7CG024400 Rh7DG023900
rosa_wichuraiana Rw7G001890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 905
AccII CGCG 3 cut(s) 200, 337, 1292
AclI AACGTT 2 cut(s) 769, 1098
AclWI GGATC 2 cut(s) 189, 1344
AcoI YGGCCR 1 cut(s) 229
AcsI RAATTY 1 cut(s) 1550
AcuI CTGAAG 1 cut(s) 558
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 4 cut(s) 211, 352, 1150, 1249
AfiI CCNNNNNNNGG 3 cut(s) 50, 905, 1087
AflII CTTAAG 1 cut(s) 1175
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 3 cut(s) 319, 1456, 1549
AhlI ACTAGT 1 cut(s) 697
AleI CACNNNNGTG 1 cut(s) 1133
AluBI AGCT 9 cut(s) 364, 805, 913, 1228, 1264, 1276, 1370, 1409, 1429
AluI AGCT 9 cut(s) 364, 805, 913, 1228, 1264, 1276, 1370, 1409, 1429
Alw21I GWGCWC 1 cut(s) 587
Alw26I GTCTC 3 cut(s) 379, 681, 710
AlwI GGATC 2 cut(s) 189, 1344
AlwNI CAGNNNCTG 1 cut(s) 1575
Ama87I CYCGRG 3 cut(s) 90, 257, 506
AoxI GGCC 3 cut(s) 229, 242, 617
ApeKI GCWGC 7 cut(s) 127, 457, 460, 969, 1207, 1273, 1520
ApoI RAATTY 1 cut(s) 1550
ArsI GACNNNNNNTTYG 8 cut(s) 634, 652, 666, 684, 818, 850, 1341, 1373
AspLEI GCGC 2 cut(s) 13, 414
AspS9I GGNCC 5 cut(s) 214, 243, 331, 1478, 1572
AsuC2I CCSGG 3 cut(s) 91, 92, 269
AsuHPI GGTGA 2 cut(s) 622, 1331
AsuII TTCGAA 1 cut(s) 653
AsuNHI GCTAGC 1 cut(s) 1276
AvaI CYCGRG 3 cut(s) 90, 257, 506
AvaII GGWCC 4 cut(s) 214, 331, 1478, 1572
AxyI CCTNAGG 1 cut(s) 429
BaeGI GKGCMC 1 cut(s) 1257
BaeI ACNNNNGTAYC 2 cut(s) 1231, 1264
Bbv12I GWGCWC 1 cut(s) 587
BbvI GCAGC 7 cut(s) 139, 444, 447, 956, 1219, 1285, 1532
BccI CCATC 4 cut(s) 865, 899, 928, 1289
BceAI ACGGC 4 cut(s) 140, 247, 316, 1091
BciVI GTATCC 1 cut(s) 485
BclI TGATCA 1 cut(s) 838
BcnI CCSGG 3 cut(s) 91, 92, 269
BcoDI GTCTC 3 cut(s) 379, 681, 710
BcuI ACTAGT 1 cut(s) 697
BfaI CTAG 2 cut(s) 698, 1277
BfoI RGCGCY 1 cut(s) 415
BfrI CTTAAG 1 cut(s) 1175
BfuI GTATCC 1 cut(s) 485
BglII AGATCT 1 cut(s) 1046
BisI GCNGC 8 cut(s) 128, 458, 461, 617, 970, 1208, 1274, 1521
BlpI GCTNAGC 1 cut(s) 1260
BlsI GCNGC 8 cut(s) 129, 459, 462, 618, 971, 1209, 1275, 1522
Bme1390I CCNGG 3 cut(s) 91, 92, 269
Bme18I GGWCC 4 cut(s) 214, 331, 1478, 1572
BmeT110I CYCGRG 3 cut(s) 90, 257, 506
BmgT120I GGNCC 5 cut(s) 214, 243, 331, 1478, 1572
BmiI GGNNCC 3 cut(s) 333, 398, 438
BmrFI CCNGG 3 cut(s) 91, 92, 269
BmrI ACTGGG 1 cut(s) 838
BmsI GCATC 4 cut(s) 1010, 1028, 1147, 1194
BmtI GCTAGC 1 cut(s) 1280
BmuI ACTGGG 1 cut(s) 838
BplI GAGNNNNNCTC 2 cut(s) 388, 420
Bpu1102I GCTNAGC 1 cut(s) 1260
Bpu14I TTCGAA 1 cut(s) 653
BpuEI CTTGAG 3 cut(s) 581, 1167, 1179
BpuMI CCSGG 3 cut(s) 91, 92, 269
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 710
BsaJI CCNNGG 5 cut(s) 90, 335, 440, 899, 979
BsaWI WCCGGW 1 cut(s) 289
BsaXI ACNNNNNCTCC 2 cut(s) 1141, 1171
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 905, 1087
Bse1I ACTGG 1 cut(s) 833
Bse21I CCTNAGG 1 cut(s) 429
Bse3DI GCAATG 2 cut(s) 963, 1360
BseDI CCNNGG 5 cut(s) 90, 335, 440, 899, 979
BseGI GGATG 5 cut(s) 448, 691, 862, 910, 1300
BseLI CCNNNNNNNGG 3 cut(s) 50, 905, 1087
BseMI GCAATG 2 cut(s) 963, 1360
BseMII CTCAG 1 cut(s) 443
BseNI ACTGG 1 cut(s) 833
BseRI GAGGAG 4 cut(s) 29, 72, 102, 417
BseSI GKGCMC 1 cut(s) 1257
BseXI GCAGC 7 cut(s) 139, 444, 447, 956, 1219, 1285, 1532
BseYI CCCAGC 1 cut(s) 1081
BsgI GTGCAG 1 cut(s) 594
Bsh1236I CGCG 3 cut(s) 200, 337, 1292
BshFI GGCC 3 cut(s) 231, 244, 619
BsiHKAI GWGCWC 1 cut(s) 587
BsiHKCI CYCGRG 3 cut(s) 90, 257, 506
BsiSI CCGG 4 cut(s) 23, 91, 268, 290
BslFI GGGAC 3 cut(s) 673, 845, 1343
BslI CCNNNNNNNGG 3 cut(s) 50, 905, 1087
BsmAI GTCTC 3 cut(s) 379, 681, 710
BsmFI GGGAC 3 cut(s) 673, 845, 1343
BsmI GAATGC 2 cut(s) 175, 487
BsnI GGCC 3 cut(s) 231, 244, 619
Bso31I GGTCTC 1 cut(s) 710
BsoBI CYCGRG 3 cut(s) 90, 257, 506
Bsp119I TTCGAA 1 cut(s) 653
Bsp1286I GDGCHC 2 cut(s) 587, 1257
Bsp143I GATC 7 cut(s) 181, 496, 838, 1046, 1117, 1336, 1381
Bsp1720I GCTNAGC 1 cut(s) 1260
BspANI GGCC 3 cut(s) 231, 244, 619
BspCNI CTCAG 1 cut(s) 442
BspFNI CGCG 3 cut(s) 200, 337, 1292
BspHI TCATGA 1 cut(s) 1564
BspLI GGNNCC 3 cut(s) 333, 398, 438
BspOI GCTAGC 1 cut(s) 1280
BspPI GGATC 2 cut(s) 189, 1344
BspT104I TTCGAA 1 cut(s) 653
BspTI CTTAAG 1 cut(s) 1175
BspTNI GGTCTC 1 cut(s) 710
BsrDI GCAATG 2 cut(s) 963, 1360
BsrI ACTGG 1 cut(s) 833
BssECI CCNNGG 5 cut(s) 90, 335, 440, 899, 979
BssMI GATC 7 cut(s) 181, 496, 838, 1046, 1117, 1336, 1381
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 2 cut(s) 899, 979
Bst4CI ACNGT 4 cut(s) 218, 1252, 1423, 1531
Bst6I CTCTTC 1 cut(s) 846
BstACI GRCGYC 1 cut(s) 303
BstAFI CTTAAG 1 cut(s) 1175
BstAPI GCANNNNNTGC 1 cut(s) 1007
BstBI TTCGAA 1 cut(s) 653
BstC8I GCNNGC 5 cut(s) 523, 911, 1257, 1278, 1474
BstDEI CTNAG 5 cut(s) 429, 601, 784, 1260, 1366
BstDSI CCRYGG 1 cut(s) 335
BstF5I GGATG 5 cut(s) 448, 691, 862, 910, 1300
BstFNI CGCG 3 cut(s) 200, 337, 1292
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 2 cut(s) 13, 414
BstKTI GATC 7 cut(s) 184, 499, 841, 1049, 1120, 1339, 1384
BstMAI GTCTC 3 cut(s) 379, 681, 710
BstMBI GATC 7 cut(s) 181, 496, 838, 1046, 1117, 1336, 1381
BstMWI GCNNNNNNNGC 7 cut(s) 32, 482, 1007, 1016, 1082, 1261, 1270
BstNSI RCATGY 1 cut(s) 160
BstSCI CCNGG 3 cut(s) 89, 90, 267
BstSLI GKGCMC 1 cut(s) 1257
BstUI CGCG 3 cut(s) 200, 337, 1292
BstV1I GCAGC 7 cut(s) 139, 444, 447, 956, 1219, 1285, 1532
BstX2I RGATCY 2 cut(s) 181, 1046
BstYI RGATCY 2 cut(s) 181, 1046
Bsu36I CCTNAGG 1 cut(s) 429
BsuI GTATCC 1 cut(s) 485
BsuRI GGCC 3 cut(s) 231, 244, 619
BtgI CCRYGG 1 cut(s) 335
BtgZI GCGATG 2 cut(s) 884, 1008
BtsCI GGATG 5 cut(s) 448, 691, 862, 910, 1300
BtsI GCAGTG 1 cut(s) 1128
BtsIMutI CAGTG 2 cut(s) 1128, 1428
Cac8I GCNNGC 5 cut(s) 523, 911, 1257, 1278, 1474
CaiI CAGNNNCTG 1 cut(s) 1575
CciI TCATGA 1 cut(s) 1564
CfoI GCGC 2 cut(s) 13, 414
Cfr13I GGNCC 5 cut(s) 214, 243, 331, 1478, 1572
Cfr42I CCGCGG 1 cut(s) 338
Cfr9I CCCGGG 1 cut(s) 90
CpoI CGGWCCG 1 cut(s) 214
CseI GACGC 1 cut(s) 292
Csp6I GTAC 4 cut(s) 210, 351, 1149, 1248
CspI CGGWCCG 1 cut(s) 214
CviAII CATG 9 cut(s) 32, 157, 240, 529, 552, 626, 631, 1201, 1565
CviQI GTAC 4 cut(s) 210, 351, 1149, 1248
DdeI CTNAG 5 cut(s) 429, 601, 784, 1260, 1366
DpnI GATC 7 cut(s) 183, 498, 840, 1048, 1119, 1338, 1383
DpnII GATC 7 cut(s) 181, 496, 838, 1046, 1117, 1336, 1381
DraI TTTAAA 1 cut(s) 1236
EaeI YGGCCR 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 846
EarI CTCTTC 1 cut(s) 846
EciI GGCGGA 1 cut(s) 449
Eco130I CCWWGG 2 cut(s) 899, 979
Eco31I GGTCTC 1 cut(s) 710
Eco47I GGWCC 4 cut(s) 214, 331, 1478, 1572
Eco57I CTGAAG 1 cut(s) 558
Eco81I CCTNAGG 1 cut(s) 429
Eco88I CYCGRG 3 cut(s) 90, 257, 506
EcoO109I RGGNCCY 2 cut(s) 331, 1572
EcoT14I CCWWGG 2 cut(s) 899, 979
ErhI CCWWGG 2 cut(s) 899, 979
FaeI CATG 9 cut(s) 35, 160, 243, 532, 555, 629, 634, 1204, 1568
FalI AAGNNNNNCTT 2 cut(s) 1253, 1285
FaqI GGGAC 3 cut(s) 673, 845, 1343
FatI CATG 9 cut(s) 31, 156, 239, 528, 551, 625, 630, 1200, 1564
FauI CCCGC 3 cut(s) 19, 342, 1264
FauNDI CATATG 1 cut(s) 589
FbaI TGATCA 1 cut(s) 838
Fnu4HI GCNGC 8 cut(s) 128, 458, 461, 617, 970, 1208, 1274, 1521
FokI GGATG 5 cut(s) 435, 698, 869, 917, 1307
Fsp4HI GCNGC 8 cut(s) 128, 458, 461, 617, 970, 1208, 1274, 1521
FspBI CTAG 2 cut(s) 698, 1277
GlaI GCGC 2 cut(s) 12, 413
GluI GCNGC 8 cut(s) 128, 458, 461, 617, 970, 1208, 1274, 1521
GsaI CCCAGC 1 cut(s) 1085
HaeII RGCGCY 1 cut(s) 415
HaeIII GGCC 3 cut(s) 231, 244, 619
HapII CCGG 4 cut(s) 23, 91, 268, 290
HgaI GACGC 1 cut(s) 292
HhaI GCGC 2 cut(s) 13, 414
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 9 cut(s) 35, 160, 243, 532, 555, 629, 634, 1204, 1568
Hin6I GCGC 2 cut(s) 11, 412
HinP1I GCGC 2 cut(s) 11, 412
HindIII AAGCTT 3 cut(s) 911, 1226, 1262
HinfI GANTC 7 cut(s) 202, 286, 445, 509, 556, 989, 1432
HpaII CCGG 4 cut(s) 23, 91, 268, 290
HphI GGTGA 2 cut(s) 622, 1331
Hpy166II GTNNAC 1 cut(s) 1414
Hpy188I TCNGA 6 cut(s) 181, 285, 391, 685, 736, 1223
Hpy188III TCNNGA 9 cut(s) 468, 506, 560, 796, 1044, 1385, 1403, 1436, 1565
Hpy8I GTNNAC 1 cut(s) 1414
Hpy99I CGWCG 3 cut(s) 308, 362, 481
HpyAV CCTTC 2 cut(s) 428, 533
HpyCH4III ACNGT 4 cut(s) 218, 1252, 1423, 1531
HpyCH4IV ACGT 2 cut(s) 769, 1098
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 482, 1007, 1016, 1082, 1261, 1270
HpyF3I CTNAG 5 cut(s) 429, 601, 784, 1260, 1366
HpySE526I ACGT 2 cut(s) 769, 1098
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 9 cut(s) 35, 160, 243, 532, 555, 629, 634, 1204, 1568
HspAI GCGC 2 cut(s) 11, 412
Ksp22I TGATCA 1 cut(s) 838
KspI CCGCGG 1 cut(s) 338
Kzo9I GATC 7 cut(s) 181, 496, 838, 1046, 1117, 1336, 1381
LmnI GCTCC 4 cut(s) 175, 396, 436, 530
Lsp1109I GCAGC 7 cut(s) 139, 444, 447, 956, 1219, 1285, 1532
LweI GCATC 4 cut(s) 1010, 1028, 1147, 1194
MaeI CTAG 2 cut(s) 698, 1277
MaeII ACGT 2 cut(s) 769, 1098
MaeIII GTNAC 4 cut(s) 824, 926, 1307, 1447
MalI GATC 7 cut(s) 183, 498, 840, 1048, 1119, 1338, 1383
MboI GATC 7 cut(s) 181, 496, 838, 1046, 1117, 1336, 1381
MboII GAAGA 8 cut(s) 99, 176, 331, 642, 800, 863, 1041, 1451
MflI RGATCY 2 cut(s) 181, 1046
MhlI GDGCHC 2 cut(s) 587, 1257
MluCI AATT 9 cut(s) 54, 645, 667, 689, 728, 813, 1122, 1161, 1550
MlyI GAGTC 2 cut(s) 295, 518
MmeI TCCRAC 1 cut(s) 200
MseI TTAA 4 cut(s) 57, 812, 1176, 1235
MslI CAYNNNNRTG 1 cut(s) 1133
MspA1I CMGCKG 2 cut(s) 337, 1476
MspCI CTTAAG 1 cut(s) 1175
MspI CCGG 4 cut(s) 23, 91, 268, 290
MspR9I CCNGG 3 cut(s) 91, 92, 269
Mva1269I GAATGC 2 cut(s) 175, 487
MvnI CGCG 3 cut(s) 200, 337, 1292
MwoI GCNNNNNNNGC 7 cut(s) 32, 482, 1007, 1016, 1082, 1261, 1270
NciI CCSGG 3 cut(s) 91, 92, 269
NdeI CATATG 1 cut(s) 589
NdeII GATC 7 cut(s) 181, 496, 838, 1046, 1117, 1336, 1381
NheI GCTAGC 1 cut(s) 1276
NlaIII CATG 9 cut(s) 35, 160, 243, 532, 555, 629, 634, 1204, 1568
NlaIV GGNNCC 3 cut(s) 333, 398, 438
NmeAIII GCCGAG 1 cut(s) 465
NmuCI GTSAC 3 cut(s) 824, 926, 1307
NspI RCATGY 1 cut(s) 160
NspV TTCGAA 1 cut(s) 653
OliI CACNNNNGTG 1 cut(s) 1133
PagI TCATGA 1 cut(s) 1564
PciI ACATGT 1 cut(s) 156
PcsI WCGNNNNNNNCGW 1 cut(s) 1104
PctI GAATGC 2 cut(s) 175, 487
PfeI GAWTC 5 cut(s) 202, 445, 556, 989, 1432
PflMI CCANNNNNTGG 1 cut(s) 905
PkrI GCNGC 8 cut(s) 129, 459, 462, 618, 971, 1209, 1275, 1522
PleI GAGTC 2 cut(s) 294, 517
PpsI GAGTC 2 cut(s) 294, 517
PpuMI RGGWCCY 2 cut(s) 331, 1572
PscI ACATGT 1 cut(s) 156
Psp1406I AACGTT 2 cut(s) 769, 1098
Psp5II RGGWCCY 2 cut(s) 331, 1572
PspFI CCCAGC 1 cut(s) 1081
PspN4I GGNNCC 3 cut(s) 333, 398, 438
PspPI GGNCC 5 cut(s) 214, 243, 331, 1478, 1572
PspPPI RGGWCCY 2 cut(s) 331, 1572
PstNI CAGNNNCTG 1 cut(s) 1575
PsuI RGATCY 2 cut(s) 181, 1046
RsaI GTAC 4 cut(s) 211, 352, 1150, 1249
RsaNI GTAC 4 cut(s) 210, 351, 1149, 1248
RseI CAYNNNNRTG 1 cut(s) 1133
Rsr2I CGGWCCG 1 cut(s) 214
RsrII CGGWCCG 1 cut(s) 214
SacII CCGCGG 1 cut(s) 338
SaqAI TTAA 4 cut(s) 57, 812, 1176, 1235
SatI GCNGC 8 cut(s) 128, 458, 461, 617, 970, 1208, 1274, 1521
Sau3AI GATC 7 cut(s) 181, 496, 838, 1046, 1117, 1336, 1381
Sau96I GGNCC 5 cut(s) 214, 243, 331, 1478, 1572
SchI GAGTC 2 cut(s) 295, 518
ScrFI CCNGG 3 cut(s) 91, 92, 269
SduI GDGCHC 2 cut(s) 587, 1257
SfaNI GCATC 4 cut(s) 1010, 1028, 1147, 1194
Sfr303I CCGCGG 1 cut(s) 338
SfuI TTCGAA 1 cut(s) 653
SgrBI CCGCGG 1 cut(s) 338
SinI GGWCC 4 cut(s) 214, 331, 1478, 1572
SmaI CCCGGG 1 cut(s) 92
SmiMI CAYNNNNRTG 1 cut(s) 1133
SmlI CTYRAG 4 cut(s) 560, 1175, 1182, 1194
SmoI CTYRAG 4 cut(s) 560, 1175, 1182, 1194
SpeI ACTAGT 1 cut(s) 697
Sse9I AATT 9 cut(s) 54, 645, 667, 689, 728, 813, 1122, 1161, 1550
SspI AATATT 1 cut(s) 66
SspMI CTAG 2 cut(s) 698, 1277
StyD4I CCNGG 3 cut(s) 89, 90, 267
StyI CCWWGG 2 cut(s) 899, 979
TaaI ACNGT 4 cut(s) 218, 1252, 1423, 1531
TaiI ACGT 2 cut(s) 772, 1101
TaqI TCGA 3 cut(s) 653, 932, 1404
TaqII GACCGA 1 cut(s) 334
TasI AATT 9 cut(s) 54, 645, 667, 689, 728, 813, 1122, 1161, 1550
TauI GCSGC 1 cut(s) 619
TfiI GAWTC 5 cut(s) 202, 445, 556, 989, 1432
Tru1I TTAA 4 cut(s) 57, 812, 1176, 1235
Tru9I TTAA 4 cut(s) 57, 812, 1176, 1235
TscAI CASTG 2 cut(s) 1135, 1428
TseFI GTSAC 3 cut(s) 824, 926, 1307
TseI GCWGC 7 cut(s) 127, 457, 460, 969, 1207, 1273, 1520
Tsp45I GTSAC 3 cut(s) 824, 926, 1307
TspDTI ATGAA 1 cut(s) 702
TspGWI ACGGA 3 cut(s) 66, 227, 441
TspMI CCCGGG 1 cut(s) 90
TspRI CASTG 2 cut(s) 1135, 1428
Van91I CCANNNNNTGG 1 cut(s) 905
Vha464I CTTAAG 1 cut(s) 1175
VpaK11BI GGWCC 4 cut(s) 214, 331, 1478, 1572
XapI RAATTY 1 cut(s) 1550
XceI RCATGY 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 2 cut(s) 390, 1167
XmaI CCCGGG 1 cut(s) 90
XspI CTAG 2 cut(s) 698, 1277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.