RLG00000005360

conserved protein UCP031088, alpha beta hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010669
Physical Location & Seq
Reverse (-)
67062580 .. 67065237
2658 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005360

Sequence Viewer

Length: 1365 bp
ATGTCGTTTCTCGTTCCCGGTTCCGATCTCCGTTCCCTCGCCACCGCTTTCGGTACTGACCGTTACTACTTCAGCCCAGTCAGGTGCCCCGAACCGCTTCGCGTCTCAGCCCGCCGCAGCCCCGCTAACGGCGTAGCCTTGGGTTTGGACAAGAGAAACGACCCGCGGTGGTCGGTTTGTACCGCCGACGAGCTTCACTTCGTCTCTGTTCCCGATTCCGACTGGAACCTCGCTCTCTGGCGCTACCTTCCCTCTCCTCAGAGCGAGCCCAGGAATCATCCGCTGTTACTGTTGTCGGGGGTCGCCACAAATGCTATTGGATTCGATCTCTCCCCTGAGTTCTCCTTTGCGCGCTCCATGTCGGCGCAAGGATATGATACCTGGATTCTTGAAGTTAGAGGTGCTGGGTTGAGCACACATGGAACCGACTCAGGGGAAGTAAATGGTTTGTTAGAAGCAGGAAAAAACCGTGCTGCACAGATTAGGGGTTTAAGTCAAACGCCATTGAACATCATGGAAGTAGGTCAACGAGCGGTTACAAGTTTTGCAGACTTCCAAGAGCGTTTTTCAACTACATTGGTAGATTTCCAGAAGCAACTTGATCTTGTCGCAAAGCTTGATTGGGACTTTGATCACTACTTGGAAGAGGATGTGCCTGTTGCGATGGAGTATATAAGAACTCAATGCAAACCAGAGGATGGGAAGCTTCTCGCAATTGGTCACTCGATGGGGGGTATCTTGCTATATGCAATGCTCTCACGATGCTCTTCTCAAGGACGAGATTCTGGATTCGCATCAGTTGTTACTTTGGGGTCATCACTTGACTACAGGCCTTCAAAATCATCACTGAAATTGTTTTTACCTCTGGCAAACCCTGCTCAGGTGGTAAATGTTCCTGTTCCGTTCGGTGCAATATATACTGCTGTGCATCATCTTGTGAGTGGTACTCCAGTTTTGTCTTGGTTAAAACCTCAAGTTTCTGCTCAAGACCTGATGGATCCAGAGTTGTATGAAAAACTTATCTTAAATAACTTTGGTACTGTCCCTGCTAAGCTTCTCTTGCAGCTAGCAACAGTCTTCCAGGAGGGTGGTTTACAGGACAGGAGTGGGACTTTCTTTTACAAGGAACATCTATGCAAAAGCAATGTCCCAATCTTGGCCCTTGGAGGAGATCAAGACCTAATATGTCCTCCTGAAGCCGTTTATGAAACAGTGAAGGTAATTCCTGAGGAATTGGTTACCTTCAAAGTTCTTGGAGAACTCAGCGGTCCTCATTATGCACACTACGATTTAGTTGGAGGTCGCGGGGCAGCAGGTGTGTATCCACACATTATTGAGTTTCTCAATCGTCATGACAGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

455

Amino Acids

49.88

Weight (kDa)

5.57

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010669)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73750 AT1G73750
fragaria_vesca FvH4_5g16710 FvH4_5g16710 FvH4_5g16710
malus_domestica MD06G1220800.v1.1
prunus_persica Prupe.5G225200_v2.0.a1 Prupe.5G225200_v2.0.a1
pyrus_communis pycom06g19670
rosa_chinensis RchiOBHm_Chr7g0179391
rosa_laevigata RLG00000005360
rosa_multiflora Rmu_sc0002581.1_g000004 Rmu_sc0019996.1_g000001
rosa_roxburghii Rroxscaffold_3G00274070
rosa_rugosa Rorug06G0424000
rosa_samantha Rh7AG023400 Rh7BG023000 Rh7CG024400 Rh7DG023900
rosa_wichuraiana Rw7G001890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1304
Acc36I ACCTGC 1 cut(s) 1304
AccB1I GGYRCC 1 cut(s) 84
AccB7I CCANNNNNTGG 1 cut(s) 698
AccBSI CCGCTC 1 cut(s) 533
AccII CGCG 4 cut(s) 102, 166, 352, 1305
AclWI GGATC 2 cut(s) 992, 1005
AcuI CTGAAG 2 cut(s) 55, 1215
AfaI GTAC 4 cut(s) 55, 181, 946, 1039
AfiI CCNNNNNNNGG 5 cut(s) 128, 432, 698, 880, 1156
AgsI TTSAA 5 cut(s) 392, 508, 570, 837, 1246
AjnI CCWGG 3 cut(s) 269, 380, 1080
AluBI AGCT 5 cut(s) 193, 616, 706, 1054, 1066
AluI AGCT 5 cut(s) 193, 616, 706, 1054, 1066
Alw21I GWGCWC 1 cut(s) 416
Alw26I GTCTC 2 cut(s) 109, 208
AlwI GGATC 2 cut(s) 992, 1005
AoxI GGCC 2 cut(s) 830, 1158
ApeKI GCWGC 4 cut(s) 117, 473, 1063, 1310
ArsI GACNNNNNNTTYG 2 cut(s) 1131, 1163
Asp700I GAANNNNTTC 1 cut(s) 96
AspLEI GCGC 4 cut(s) 243, 352, 354, 367
AspS9I GGNCC 2 cut(s) 1159, 1268
AsuC2I CCSGG 1 cut(s) 18
AsuNHI GCTAGC 1 cut(s) 1066
AvaII GGWCC 1 cut(s) 1268
AxyI CCTNAGG 1 cut(s) 1227
BaeGI GKGCMC 1 cut(s) 89
BaeI ACNNNNGTAYC 2 cut(s) 936, 969
BamHI GGATCC 1 cut(s) 997
BanI GGYRCC 1 cut(s) 84
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 1069
Bbv12I GWGCWC 1 cut(s) 416
BbvI GCAGC 4 cut(s) 129, 460, 1075, 1322
BccI CCATC 4 cut(s) 658, 692, 721, 988
BceAI ACGGC 2 cut(s) 145, 1184
BciT130I CCWGG 3 cut(s) 271, 382, 1082
BciVI GTATCC 1 cut(s) 1332
BclI TGATCA 1 cut(s) 631
BcnI CCSGG 1 cut(s) 18
BcoDI GTCTC 2 cut(s) 109, 208
BfaI CTAG 1 cut(s) 1067
BfmI CTRYAG 1 cut(s) 826
BfoI RGCGCY 1 cut(s) 244
BfuAI ACCTGC 1 cut(s) 1304
BfuI GTATCC 1 cut(s) 1332
BisI GCNGC 5 cut(s) 115, 118, 474, 1064, 1311
BlpI GCTNAGC 1 cut(s) 1050
BlsI GCNGC 5 cut(s) 116, 119, 475, 1065, 1312
Bme1390I CCNGG 4 cut(s) 18, 271, 382, 1082
Bme18I GGWCC 1 cut(s) 1268
BmgT120I GGNCC 2 cut(s) 1159, 1268
BmiI GGNNCC 5 cut(s) 22, 86, 227, 424, 999
BmrFI CCNGG 4 cut(s) 18, 271, 382, 1082
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 3 cut(s) 752, 803, 937
BmtI GCTAGC 1 cut(s) 1070
BmuI ACTGGG 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 1069
BplI GAGNNNNNCTC 2 cut(s) 931, 963
BpmI CTGGAG 1 cut(s) 933
Bpu10I CCTNAGC 1 cut(s) 879
Bpu1102I GCTNAGC 1 cut(s) 1050
BpuEI CTTGAG 3 cut(s) 756, 957, 969
BpuMI CCSGG 1 cut(s) 18
BsaBI GATNNNNATC 1 cut(s) 793
BsaJI CCNNGG 4 cut(s) 138, 164, 269, 1162
Bsc4I CCNNNNNNNGG 5 cut(s) 128, 432, 698, 880, 1156
Bse1I ACTGG 3 cut(s) 77, 227, 950
Bse21I CCTNAGG 1 cut(s) 1227
Bse3DI GCAATG 2 cut(s) 756, 1150
Bse8I GATNNNNATC 1 cut(s) 793
BseBI CCWGG 3 cut(s) 271, 382, 1082
BseDI CCNNGG 4 cut(s) 138, 164, 269, 1162
BseGI GGATG 3 cut(s) 277, 655, 703
BseJI GATNNNNATC 1 cut(s) 793
BseLI CCNNNNNNNGG 5 cut(s) 128, 432, 698, 880, 1156
BseMI GCAATG 2 cut(s) 756, 1150
BseMII CTCAG 7 cut(s) 120, 272, 327, 444, 893, 1218, 1276
BseNI ACTGG 3 cut(s) 77, 227, 950
BsePI GCGCGC 1 cut(s) 350
BseRI GAGGAG 2 cut(s) 246, 1182
BseSI GKGCMC 1 cut(s) 89
BseXI GCAGC 4 cut(s) 129, 460, 1075, 1322
BseYI CCCAGC 1 cut(s) 404
BsgI GTGCAG 1 cut(s) 459
Bsh1236I CGCG 4 cut(s) 102, 166, 352, 1305
BshFI GGCC 2 cut(s) 832, 1160
BshNI GGYRCC 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 416
BsiSI CCGG 1 cut(s) 18
BslFI GGGAC 4 cut(s) 638, 1028, 1123, 1133
BslI CCNNNNNNNGG 5 cut(s) 128, 432, 698, 880, 1156
BsmAI GTCTC 2 cut(s) 109, 208
BsmBI CGTCTC 2 cut(s) 109, 208
BsmFI GGGAC 4 cut(s) 638, 1028, 1123, 1133
BsnI GGCC 2 cut(s) 832, 1160
Bsp1286I GDGCHC 3 cut(s) 89, 270, 416
Bsp143I GATC 6 cut(s) 25, 325, 601, 631, 997, 1171
Bsp1720I GCTNAGC 1 cut(s) 1050
BspANI GGCC 2 cut(s) 832, 1160
BspCNI CTCAG 7 cut(s) 119, 271, 328, 443, 892, 1219, 1275
BspFNI CGCG 4 cut(s) 102, 166, 352, 1305
BspHI TCATGA 1 cut(s) 1351
BspLI GGNNCC 5 cut(s) 22, 86, 227, 424, 999
BspMI ACCTGC 1 cut(s) 1304
BspOI GCTAGC 1 cut(s) 1070
BspPI GGATC 2 cut(s) 992, 1005
BspQI GCTCTTC 1 cut(s) 772
BspT107I GGYRCC 1 cut(s) 84
BsrBI CCGCTC 1 cut(s) 533
BsrDI GCAATG 2 cut(s) 756, 1150
BsrI ACTGG 3 cut(s) 77, 227, 950
BssECI CCNNGG 4 cut(s) 138, 164, 269, 1162
BssHII GCGCGC 1 cut(s) 350
BssMI GATC 6 cut(s) 25, 325, 601, 631, 997, 1171
BssT1I CCWWGG 2 cut(s) 138, 1162
Bst2UI CCWGG 3 cut(s) 271, 382, 1082
Bst4CI ACNGT 6 cut(s) 62, 291, 470, 1042, 1075, 1213
Bst6I CTCTTC 2 cut(s) 639, 772
BstAPI GCANNNNNTGC 1 cut(s) 875
BstC8I GCNNGC 4 cut(s) 112, 266, 352, 1068
BstDEI CTNAG 8 cut(s) 106, 258, 336, 430, 879, 1050, 1227, 1262
BstDSI CCRYGG 1 cut(s) 164
BstEII GGTNACC 1 cut(s) 1237
BstF5I GGATG 3 cut(s) 277, 655, 703
BstFNI CGCG 4 cut(s) 102, 166, 352, 1305
BstH2I RGCGCY 1 cut(s) 244
BstHHI GCGC 4 cut(s) 243, 352, 354, 367
BstKTI GATC 6 cut(s) 28, 328, 604, 634, 1000, 1174
BstMAI GTCTC 2 cut(s) 109, 208
BstMBI GATC 6 cut(s) 25, 325, 601, 631, 997, 1171
BstMWI GCNNNNNNNGC 3 cut(s) 311, 875, 1060
BstNI CCWGG 3 cut(s) 271, 382, 1082
BstPI GGTNACC 1 cut(s) 1237
BstSCI CCNGG 4 cut(s) 16, 269, 380, 1080
BstSFI CTRYAG 1 cut(s) 826
BstSLI GKGCMC 1 cut(s) 89
BstUI CGCG 4 cut(s) 102, 166, 352, 1305
BstV1I GCAGC 4 cut(s) 129, 460, 1075, 1322
BstV2I GAAGAC 1 cut(s) 1069
BstX2I RGATCY 1 cut(s) 997
BstXI CCANNNNNNTGG 1 cut(s) 1088
BstYI RGATCY 1 cut(s) 997
Bsu36I CCTNAGG 1 cut(s) 1227
BsuI GTATCC 1 cut(s) 1332
BsuRI GGCC 2 cut(s) 832, 1160
BtgI CCRYGG 1 cut(s) 164
BtgZI GCGATG 1 cut(s) 677
BtsCI GGATG 3 cut(s) 277, 655, 703
BtsIMutI CAGTG 2 cut(s) 845, 1218
BveI ACCTGC 1 cut(s) 1304
Cac8I GCNNGC 4 cut(s) 112, 266, 352, 1068
CciI TCATGA 1 cut(s) 1351
CfoI GCGC 4 cut(s) 243, 352, 354, 367
Cfr13I GGNCC 2 cut(s) 1159, 1268
Cfr42I CCGCGG 1 cut(s) 167
CseI GACGC 1 cut(s) 91
Csp6I GTAC 4 cut(s) 54, 180, 945, 1038
CviAII CATG 4 cut(s) 358, 419, 514, 1352
CviQI GTAC 4 cut(s) 54, 180, 945, 1038
DdeI CTNAG 8 cut(s) 106, 258, 336, 430, 879, 1050, 1227, 1262
DpnI GATC 6 cut(s) 27, 327, 603, 633, 999, 1173
DpnII GATC 6 cut(s) 25, 325, 601, 631, 997, 1171
Eam1104I CTCTTC 2 cut(s) 639, 772
EarI CTCTTC 2 cut(s) 639, 772
Eco130I CCWWGG 2 cut(s) 138, 1162
Eco147I AGGCCT 1 cut(s) 832
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 1268
Eco57I CTGAAG 2 cut(s) 55, 1215
Eco81I CCTNAGG 1 cut(s) 1227
Eco91I GGTNACC 1 cut(s) 1237
EcoO65I GGTNACC 1 cut(s) 1237
EcoRII CCWGG 3 cut(s) 269, 380, 1080
EcoT14I CCWWGG 2 cut(s) 138, 1162
EcoT38I GRGCYC 1 cut(s) 270
ErhI CCWWGG 2 cut(s) 138, 1162
Esp3I CGTCTC 2 cut(s) 109, 208
FaeI CATG 4 cut(s) 361, 422, 517, 1355
FalI AAGNNNNNCTT 2 cut(s) 1043, 1075
FaqI GGGAC 4 cut(s) 638, 1028, 1123, 1133
FatI CATG 4 cut(s) 357, 418, 513, 1351
FauI CCCGC 4 cut(s) 119, 130, 171, 1298
FbaI TGATCA 1 cut(s) 631
Fnu4HI GCNGC 5 cut(s) 115, 118, 474, 1064, 1311
FokI GGATG 3 cut(s) 264, 662, 710
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 5 cut(s) 115, 118, 474, 1064, 1311
FspBI CTAG 1 cut(s) 1067
GlaI GCGC 4 cut(s) 242, 351, 353, 366
GluI GCNGC 5 cut(s) 115, 118, 474, 1064, 1311
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 933
HaeII RGCGCY 1 cut(s) 244
HaeIII GGCC 2 cut(s) 832, 1160
HapII CCGG 1 cut(s) 18
HgaI GACGC 1 cut(s) 91
HhaI GCGC 4 cut(s) 243, 352, 354, 367
Hin1II CATG 4 cut(s) 361, 422, 517, 1355
Hin6I GCGC 4 cut(s) 241, 350, 352, 365
HinP1I GCGC 4 cut(s) 241, 350, 352, 365
HincII GTYRAC 1 cut(s) 527
HindII GTYRAC 1 cut(s) 527
HindIII AAGCTT 3 cut(s) 614, 704, 1052
HinfI GANTC 7 cut(s) 215, 274, 321, 385, 428, 782, 789
HpaII CCGG 1 cut(s) 18
Hpy166II GTNNAC 2 cut(s) 527, 1094
Hpy188I TCNGA 3 cut(s) 25, 220, 261
Hpy8I GTNNAC 2 cut(s) 527, 1094
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 4 cut(s) 257, 843, 1210, 1252
HpyCH4III ACNGT 6 cut(s) 62, 291, 470, 1042, 1075, 1213
HpyCH4V TGCA 9 cut(s) 476, 548, 687, 749, 911, 928, 1063, 1137, 1280
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 875, 1060
HpyF3I CTNAG 8 cut(s) 106, 258, 336, 430, 879, 1050, 1227, 1262
Hsp92II CATG 4 cut(s) 361, 422, 517, 1355
HspAI GCGC 4 cut(s) 241, 350, 352, 365
Ksp22I TGATCA 1 cut(s) 631
KspI CCGCGG 1 cut(s) 167
Kzo9I GATC 6 cut(s) 25, 325, 601, 631, 997, 1171
LguI GCTCTTC 1 cut(s) 772
LmnI GCTCC 1 cut(s) 359
Lsp1109I GCAGC 4 cut(s) 129, 460, 1075, 1322
LweI GCATC 3 cut(s) 752, 803, 937
MaeI CTAG 1 cut(s) 1067
MaeIII GTNAC 6 cut(s) 62, 285, 535, 719, 802, 1237
MalI GATC 6 cut(s) 27, 327, 603, 633, 999, 1173
MbiI CCGCTC 1 cut(s) 533
MboI GATC 6 cut(s) 25, 325, 601, 631, 997, 1171
MboII GAAGA 3 cut(s) 656, 759, 1069
MfeI CAATTG 1 cut(s) 714
MflI RGATCY 1 cut(s) 997
MhlI GDGCHC 3 cut(s) 89, 270, 416
MluCI AATT 4 cut(s) 714, 851, 1221, 1232
MlyI GAGTC 1 cut(s) 422
MmeI TCCRAC 2 cut(s) 243, 1276
MroXI GAANNNNTTC 1 cut(s) 96
MseI TTAA 3 cut(s) 491, 965, 1025
MspA1I CMGCKG 3 cut(s) 166, 283, 1266
MspI CCGG 1 cut(s) 18
MspR9I CCNGG 4 cut(s) 18, 271, 382, 1082
MunI CAATTG 1 cut(s) 714
MvaI CCWGG 3 cut(s) 271, 382, 1082
MvnI CGCG 4 cut(s) 102, 166, 352, 1305
MwoI GCNNNNNNNGC 3 cut(s) 311, 875, 1060
NciI CCSGG 1 cut(s) 18
NdeII GATC 6 cut(s) 25, 325, 601, 631, 997, 1171
NheI GCTAGC 1 cut(s) 1066
NlaIII CATG 4 cut(s) 361, 422, 517, 1355
NlaIV GGNNCC 5 cut(s) 22, 86, 227, 424, 999
NmuCI GTSAC 1 cut(s) 719
PagI TCATGA 1 cut(s) 1351
PaqCI CACCTGC 1 cut(s) 1304
PauI GCGCGC 1 cut(s) 350
PceI AGGCCT 1 cut(s) 832
PciSI GCTCTTC 1 cut(s) 772
PdmI GAANNNNTTC 1 cut(s) 96
PfeI GAWTC 6 cut(s) 215, 274, 321, 385, 782, 789
PflMI CCANNNNNTGG 1 cut(s) 698
PfoI TCCNGGA 1 cut(s) 1080
PkrI GCNGC 5 cut(s) 116, 119, 475, 1065, 1312
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
Psp6I CCWGG 3 cut(s) 269, 380, 1080
PspEI GGTNACC 1 cut(s) 1237
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 3 cut(s) 269, 380, 1080
PspN4I GGNNCC 5 cut(s) 22, 86, 227, 424, 999
PspPI GGNCC 2 cut(s) 1159, 1268
PsuI RGATCY 1 cut(s) 997
PteI GCGCGC 1 cut(s) 350
RsaI GTAC 4 cut(s) 55, 181, 946, 1039
RsaNI GTAC 4 cut(s) 54, 180, 945, 1038
SacII CCGCGG 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 772
SaqAI TTAA 3 cut(s) 491, 965, 1025
SatI GCNGC 5 cut(s) 115, 118, 474, 1064, 1311
Sau3AI GATC 6 cut(s) 25, 325, 601, 631, 997, 1171
Sau96I GGNCC 2 cut(s) 1159, 1268
SchI GAGTC 1 cut(s) 422
ScrFI CCNGG 4 cut(s) 18, 271, 382, 1082
SduI GDGCHC 3 cut(s) 89, 270, 416
SfaNI GCATC 3 cut(s) 752, 803, 937
SfcI CTRYAG 1 cut(s) 826
Sfr303I CCGCGG 1 cut(s) 167
SgrBI CCGCGG 1 cut(s) 167
SinI GGWCC 1 cut(s) 1268
SmlI CTYRAG 3 cut(s) 771, 972, 984
SmoI CTYRAG 3 cut(s) 771, 972, 984
Sse9I AATT 4 cut(s) 714, 851, 1221, 1232
SseBI AGGCCT 1 cut(s) 832
SspMI CTAG 1 cut(s) 1067
StuI AGGCCT 1 cut(s) 832
StyD4I CCNGG 4 cut(s) 16, 269, 380, 1080
StyI CCWWGG 2 cut(s) 138, 1162
TaaI ACNGT 6 cut(s) 62, 291, 470, 1042, 1075, 1213
TaqI TCGA 2 cut(s) 324, 725
TasI AATT 4 cut(s) 714, 851, 1221, 1232
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 6 cut(s) 215, 274, 321, 385, 782, 789
Tru1I TTAA 3 cut(s) 491, 965, 1025
Tru9I TTAA 3 cut(s) 491, 965, 1025
TscAI CASTG 2 cut(s) 852, 1218
TseFI GTSAC 1 cut(s) 719
TseI GCWGC 4 cut(s) 117, 473, 1063, 1310
Tsp45I GTSAC 1 cut(s) 719
TspDTI ATGAA 2 cut(s) 1026, 1221
TspGWI ACGGA 2 cut(s) 20, 891
TspRI CASTG 2 cut(s) 852, 1218
Van91I CCANNNNNTGG 1 cut(s) 698
VpaK11BI GGWCC 1 cut(s) 1268
XcmI CCANNNNNNNNNTGG 1 cut(s) 957
XmnI GAANNNNTTC 1 cut(s) 96
XspI CTAG 1 cut(s) 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.