Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0018882

Physical Location & Seq

Genomic Coordinates Reverse (-)

36609535 .. 36611315

Gene Structure 1781 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1235200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 852 bp

ATGGGGAAGACCATGGCGGTGGGAGTACACTGCATCATCGCCTTGCTAATTCTGTCCGGTGCGGATCTAGTCAGGTCCGACGGGTCCGATCATCGGTACAAGGAAGGAGATTTTGTCCCTCTGTATGCCAACAAGGTCGGCCCGTTTCACAACCCCAGTGAAACGTACCGATACTTTGATCTCCCATTCTGCTCACCAGATGCGGTGAAAGAGAAAAGGGAAGCTCTTGGTGAAGCATTGAATGGGGATCGTTTAGTCAGTGCCCCTTACAAACTTGAGTTCCTAAGGCATAAAGATACCGAAGTAGCTTGCAAGAAAACACTCACAAAGGAAGAAGTAGCCCGGTTCAGATCTGCTGTCAACAAGGACTATTACTTCCAGATGTATTATGATGACTTGCCCACTTGGGGTTTCATAGGGAAGGTTGACAAGGAAGGCAAAGACCCGAGCGAGTACAAGACCTACCTTTATGAGCACATTCATTTTGATCTCTATTATAACAAAGAACGCGTGATTGAAGTCAATATTCGAACAGATCCAAATGCACTGGTTGACCTTACTGAGGATAAAGAAGTTGATGTTGAATTCATGTATACTGTGGAGTGGAAGGAAACAACCACTCCTTTCGATAAGAGGATGGATAAGTATTCTCAGTCCTCATCCTTGCCTCCTCTCGTCAATGCCTATGAATTATATACTATCACTAGACCTCTAATCACCAACCCTAAAGATGTGGAAGGGCTAGAAAAACTGGGAAGTGTATTTGAAGGACTGGAAAGAGGATATGATACGCAAGATAAACTTAATGGTGATCCTTGGTCTTTCAGCCTCGTTAGTAGTGAACTGGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002790 GO:0003674 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006897 GO:0006909 GO:0007154 GO:0007155 GO:0007165 GO:0008104 GO:0008150 GO:0008283 GO:0009306 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016192 GO:0022610 GO:0023052 GO:0030587 GO:0031224 GO:0031226 GO:0031589 GO:0032502 GO:0032940 GO:0033036 GO:0033299 GO:0034613 GO:0038023 GO:0042886 GO:0044425 GO:0044459 GO:0044464 GO:0045184 GO:0046903 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051703 GO:0051704 GO:0051716 GO:0060089 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0072657 GO:0090702 GO:0098657 GO:0099120

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EMP70

Protein Analysis

284

Amino Acids

32.62

Weight (kDa)

5.02

Isoelectric Point (pI)

39.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMP70	PF02990	55 - 219	2.2e-39	Endomembrane protein 70

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Publication-ready

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Orthologous Genes (Group: OG0018882)

Species	Orthologous Gene IDs
malus_domestica	MD06G1235000.v1.1 MD06G1235200.v1.1
rosa_multiflora	Rmu_co8090820.1_g000001 Rmu_sc0000016.1_g000004 Rmu_sc0000112.1_g000027 Rmu_sc0009988.1_g000017 Rmu_sc0019843.1_g000001 Rmu_sc0040871.1_g000001 Rmu_ssc0000126.1_g000005

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	498
AccI	GTMKAC	1 cut(s)	593
AccII	CGCG	1 cut(s)	510
AciI	CCGC	3 cut(s)	17, 62, 203
AclWI	GGATC	4 cut(s)	72, 255, 530, 806
AcsI	RAATTY	1 cut(s)	584
AfaI	GTAC	4 cut(s)	27, 98, 167, 455
AfiI	CCNNNNNNNGG	3 cut(s)	93, 407, 562
AflIII	ACRYGT	1 cut(s)	508
AgsI	TTSAA	4 cut(s)	241, 518, 584, 767
AluBI	AGCT	2 cut(s)	224, 308
AluI	AGCT	2 cut(s)	224, 308
Alw21I	GWGCWC	1 cut(s)	477
AlwI	GGATC	4 cut(s)	72, 255, 530, 806
Ama87I	CYCGRG	1 cut(s)	445
AoxI	GGCC	1 cut(s)	139
ApoI	RAATTY	1 cut(s)	584
AspS9I	GGNCC	3 cut(s)	75, 84, 140
AsuC2I	CCSGG	1 cut(s)	343
AsuHPI	GGTGA	5 cut(s)	186, 217, 242, 709, 821
AsuII	TTCGAA	1 cut(s)	529
AvaI	CYCGRG	1 cut(s)	445
AvaII	GGWCC	2 cut(s)	75, 84
AxyI	CCTNAGG	1 cut(s)	284
BaeGI	GKGCMC	1 cut(s)	265
BaeI	ACNNNNGTAYC	2 cut(s)	288, 321
BbsI	GAAGAC	1 cut(s)	14
Bbv12I	GWGCWC	1 cut(s)	477
BccI	CCATC	1 cut(s)	631
BcnI	CCSGG	1 cut(s)	343
BfaI	CTAG	3 cut(s)	68, 705, 743
BglII	AGATCT	1 cut(s)	350
Bme1390I	CCNGG	1 cut(s)	343
Bme18I	GGWCC	2 cut(s)	75, 84
BmeT110I	CYCGRG	1 cut(s)	445
BmgT120I	GGNCC	3 cut(s)	75, 84, 140
BmiI	GGNNCC	1 cut(s)	85
BmrFI	CCNGG	1 cut(s)	343
BmrI	ACTGGG	2 cut(s)	150, 761
BmsI	GCATC	2 cut(s)	42, 190
BmuI	ACTGGG	2 cut(s)	150, 761
BpiI	GAAGAC	1 cut(s)	14
Bpu14I	TTCGAA	1 cut(s)	529
BpuEI	CTTGAG	1 cut(s)	296
BpuMI	CCSGG	1 cut(s)	343
BsaJI	CCNNGG	2 cut(s)	12, 815
BsaWI	WCCGGW	1 cut(s)	56
BsaXI	ACNNNNNCTCC	4 cut(s)	99, 129, 604, 634
Bsc4I	CCNNNNNNNGG	3 cut(s)	93, 407, 562
Bse1I	ACTGG	5 cut(s)	156, 552, 756, 777, 849
Bse21I	CCTNAGG	1 cut(s)	284
BseDI	CCNNGG	2 cut(s)	12, 815
BseGI	GGATG	2 cut(s)	642, 659
BseLI	CCNNNNNNNGG	3 cut(s)	93, 407, 562
BseMII	CTCAG	2 cut(s)	552, 665
BseNI	ACTGG	5 cut(s)	156, 552, 756, 777, 849
BseRI	GAGGAG	1 cut(s)	660
BseSI	GKGCMC	1 cut(s)	265
Bsh1236I	CGCG	1 cut(s)	510
BshFI	GGCC	1 cut(s)	141
BsiHKAI	GWGCWC	1 cut(s)	477
BsiHKCI	CYCGRG	1 cut(s)	445
BsiSI	CCGG	2 cut(s)	57, 343
BslFI	GGGAC	1 cut(s)	101
BslI	CCNNNNNNNGG	3 cut(s)	93, 407, 562
BsmFI	GGGAC	1 cut(s)	101
BsnI	GGCC	1 cut(s)	141
BsoBI	CYCGRG	1 cut(s)	445
Bsp119I	TTCGAA	1 cut(s)	529
Bsp1286I	GDGCHC	2 cut(s)	265, 477
Bsp143I	GATC	8 cut(s)	64, 88, 178, 247, 350, 487, 535, 811
Bsp19I	CCATGG	1 cut(s)	12
BspACI	CCGC	3 cut(s)	17, 62, 203
BspANI	GGCC	1 cut(s)	141
BspCNI	CTCAG	2 cut(s)	553, 664
BspFNI	CGCG	1 cut(s)	510
BspLI	GGNNCC	1 cut(s)	85
BspPI	GGATC	4 cut(s)	72, 255, 530, 806
BspT104I	TTCGAA	1 cut(s)	529
BsrI	ACTGG	5 cut(s)	156, 552, 756, 777, 849
BssECI	CCNNGG	2 cut(s)	12, 815
BssMI	GATC	8 cut(s)	64, 88, 178, 247, 350, 487, 535, 811
BssNAI	GTATAC	1 cut(s)	594
BssT1I	CCWWGG	2 cut(s)	12, 815
Bst1107I	GTATAC	1 cut(s)	594
Bst4CI	ACNGT	1 cut(s)	598
BstBI	TTCGAA	1 cut(s)	529
BstC8I	GCNNGC	1 cut(s)	310
BstDEI	CTNAG	3 cut(s)	284, 561, 651
BstDSI	CCRYGG	1 cut(s)	12
BstENI	CCTNNNNNAGG	1 cut(s)	560
BstF5I	GGATG	2 cut(s)	642, 659
BstFNI	CGCG	1 cut(s)	510
BstKTI	GATC	8 cut(s)	67, 91, 181, 250, 353, 490, 538, 814
BstMBI	GATC	8 cut(s)	64, 88, 178, 247, 350, 487, 535, 811
BstSCI	CCNGG	1 cut(s)	341
BstSLI	GKGCMC	1 cut(s)	265
BstUI	CGCG	1 cut(s)	510
BstV2I	GAAGAC	1 cut(s)	14
BstX2I	RGATCY	3 cut(s)	64, 350, 535
BstXI	CCANNNNNNTGG	1 cut(s)	19
BstYI	RGATCY	3 cut(s)	64, 350, 535
BstZ17I	GTATAC	1 cut(s)	594
Bsu36I	CCTNAGG	1 cut(s)	284
BsuRI	GGCC	1 cut(s)	141
BtgI	CCRYGG	1 cut(s)	12
BtgZI	GCGATG	1 cut(s)	22
BtsCI	GGATG	2 cut(s)	642, 659
BtsI	GCAGTG	1 cut(s)	28
BtsIMutI	CAGTG	4 cut(s)	28, 163, 265, 545
Cac8I	GCNNGC	1 cut(s)	310
Cfr13I	GGNCC	3 cut(s)	75, 84, 140
Csp6I	GTAC	4 cut(s)	26, 97, 166, 454
CviAII	CATG	2 cut(s)	13, 589
CviJI	RGCY	6 cut(s)	141, 224, 308, 341, 742, 828
CviKI_1	RGCY	6 cut(s)	141, 224, 308, 341, 742, 828
CviQI	GTAC	4 cut(s)	26, 97, 166, 454
DdeI	CTNAG	3 cut(s)	284, 561, 651
DpnI	GATC	8 cut(s)	66, 90, 180, 249, 352, 489, 537, 813
DpnII	GATC	8 cut(s)	64, 88, 178, 247, 350, 487, 535, 811
Eco130I	CCWWGG	2 cut(s)	12, 815
Eco47I	GGWCC	2 cut(s)	75, 84
Eco81I	CCTNAGG	1 cut(s)	284
Eco88I	CYCGRG	1 cut(s)	445
EcoNI	CCTNNNNNAGG	1 cut(s)	560
EcoRI	GAATTC	1 cut(s)	584
EcoT14I	CCWWGG	2 cut(s)	12, 815
ErhI	CCWWGG	2 cut(s)	12, 815
FaeI	CATG	2 cut(s)	16, 592
FalI	AAGNNNNNCTT	2 cut(s)	786, 818
FaqI	GGGAC	1 cut(s)	101
FatI	CATG	2 cut(s)	12, 588
FblI	GTMKAC	1 cut(s)	593
FokI	GGATG	2 cut(s)	646, 649
FspBI	CTAG	3 cut(s)	68, 705, 743
HaeIII	GGCC	1 cut(s)	141
HapII	CCGG	2 cut(s)	57, 343
Hin1II	CATG	2 cut(s)	16, 592
HincII	GTYRAC	3 cut(s)	361, 427, 553
HindII	GTYRAC	3 cut(s)	361, 427, 553
HpaII	CCGG	2 cut(s)	57, 343
HphI	GGTGA	5 cut(s)	186, 217, 242, 709, 821
Hpy166II	GTNNAC	6 cut(s)	28, 361, 427, 553, 594, 842
Hpy188I	TCNGA	3 cut(s)	79, 88, 350
Hpy188III	TCNNGA	1 cut(s)	379
Hpy8I	GTNNAC	6 cut(s)	28, 361, 427, 553, 594, 842
Hpy99I	CGWCG	1 cut(s)	83
HpyAV	CCTTC	6 cut(s)	98, 415, 428, 601, 731, 761
HpyCH4III	ACNGT	1 cut(s)	598
HpyCH4IV	ACGT	1 cut(s)	164
HpyCH4V	TGCA	3 cut(s)	33, 312, 545
HpyF3I	CTNAG	3 cut(s)	284, 561, 651
HpySE526I	ACGT	1 cut(s)	164
Hsp92II	CATG	2 cut(s)	16, 592
Kzo9I	GATC	8 cut(s)	64, 88, 178, 247, 350, 487, 535, 811
LweI	GCATC	2 cut(s)	42, 190
MaeI	CTAG	3 cut(s)	68, 705, 743
MaeII	ACGT	1 cut(s)	164
MalI	GATC	8 cut(s)	66, 90, 180, 249, 352, 489, 537, 813
MboI	GATC	8 cut(s)	64, 88, 178, 247, 350, 487, 535, 811
MboII	GAAGA	2 cut(s)	19, 344
MflI	RGATCY	3 cut(s)	64, 350, 535
MhlI	GDGCHC	2 cut(s)	265, 477
MluCI	AATT	3 cut(s)	48, 584, 689
MluI	ACGCGT	1 cut(s)	508
MmeI	TCCRAC	1 cut(s)	102
MnlI	CCTC	9 cut(s)	129, 556, 627, 667, 678, 681, 720, 773, 839
MseI	TTAA	1 cut(s)	804
MslI	CAYNNNNRTG	1 cut(s)	17
MspI	CCGG	2 cut(s)	57, 343
MspR9I	CCNGG	1 cut(s)	343
MvnI	CGCG	1 cut(s)	510
NciI	CCSGG	1 cut(s)	343
NcoI	CCATGG	1 cut(s)	12
NdeII	GATC	8 cut(s)	64, 88, 178, 247, 350, 487, 535, 811
NlaIII	CATG	2 cut(s)	16, 592
NlaIV	GGNNCC	1 cut(s)	85
NspV	TTCGAA	1 cut(s)	529
PsiI	TTATAA	1 cut(s)	498
PspN4I	GGNNCC	1 cut(s)	85
PspPI	GGNCC	3 cut(s)	75, 84, 140
PsuI	RGATCY	3 cut(s)	64, 350, 535
RsaI	GTAC	4 cut(s)	27, 98, 167, 455
RsaNI	GTAC	4 cut(s)	26, 97, 166, 454
RseI	CAYNNNNRTG	1 cut(s)	17
SaqAI	TTAA	1 cut(s)	804
Sau3AI	GATC	8 cut(s)	64, 88, 178, 247, 350, 487, 535, 811
Sau96I	GGNCC	3 cut(s)	75, 84, 140
ScrFI	CCNGG	1 cut(s)	343
SduI	GDGCHC	2 cut(s)	265, 477
SfaNI	GCATC	2 cut(s)	42, 190
SfuI	TTCGAA	1 cut(s)	529
SinI	GGWCC	2 cut(s)	75, 84
SmiMI	CAYNNNNRTG	1 cut(s)	17
SmlI	CTYRAG	1 cut(s)	275
SmoI	CTYRAG	1 cut(s)	275
Sse9I	AATT	3 cut(s)	48, 584, 689
SsiI	CCGC	3 cut(s)	17, 62, 203
SspI	AATATT	1 cut(s)	526
SspMI	CTAG	3 cut(s)	68, 705, 743
StyD4I	CCNGG	1 cut(s)	341
StyI	CCWWGG	2 cut(s)	12, 815
TaaI	ACNGT	1 cut(s)	598
TaiI	ACGT	1 cut(s)	167
TaqI	TCGA	2 cut(s)	529, 627
TasI	AATT	3 cut(s)	48, 584, 689
TatI	WGTACW	2 cut(s)	25, 453
Tru1I	TTAA	1 cut(s)	804
Tru9I	TTAA	1 cut(s)	804
TscAI	CASTG	4 cut(s)	35, 163, 265, 552
TspDTI	ATGAA	4 cut(s)	403, 470, 577, 702
TspRI	CASTG	4 cut(s)	35, 163, 265, 552
VpaK11BI	GGWCC	2 cut(s)	75, 84
XagI	CCTNNNNNAGG	1 cut(s)	560
XapI	RAATTY	1 cut(s)	584
XmiI	GTMKAC	1 cut(s)	593
XspI	CTAG	3 cut(s)	68, 705, 743

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.