MD06G1236800.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008042
Physical Location & Seq
Reverse (-)
36799156 .. 36801479
2324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1236800.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGCGCCAGTGGGATTACCTCCCGGTTTTAGGTTCCACCCGACGGACGAGGAGCTGGTGAATTATTATCTCAAGAGGAAAATCAACGGCCAAGAGATTGAGCTCGACATCATTCCTGAAGTTGATCTCTACAAATGCGAGCCTTGGGAATTAGCAGAAAAATCGTTTTTGCCAAGTAGAGACCCGGAGTGGTACTTCTTTGGACCGCGGGATCGAAAATACCCTAATGGCTTCAGAACAAACAGAGCAACGAGAGCAGGATATTGGAAGTCGACTGGAAAAGACAGGAGAGTGACGTGCCAGAATCGAGCGATCGGAATGAAGAAAACGCTGGTTTACTACAGAGGCCGAGCTCCTCAGGGCATTCGGACTGACTGGGTCATGCACGAGTATCGCCTCGACGACAAGGACTACTACTGCTCCTCCGACCCCGACACCTCCTCCTCATCTGCCGCAATTCAGATTTTTCATCTCGGAATGATGGATAACCGTGCTTGCCACCAGTTATCCCCCTTGAAAAAGAAAAAAAAACAAGAAGATTTTTCACGAATAACTAAAATGTTTACCGTAAATAAACTTAAAGATAACTTTTATCGATTTTTATTTTTTGAGAGATCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

208

Amino Acids

24.62

Weight (kDa)

9.46

Isoelectric Point (pI)

39.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 7 - 133 6.1e-50 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 272
AccII CGCG 1 cut(s) 208
AciI CCGC 3 cut(s) 206, 208, 453
AclWI GGATC 1 cut(s) 219
AcoI YGGCCR 1 cut(s) 88
AcuI CTGAAG 2 cut(s) 138, 217
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 2 cut(s) 30, 43
AgsI TTSAA 1 cut(s) 517
AjiI CACGTC 1 cut(s) 297
AluBI AGCT 3 cut(s) 55, 103, 353
AluI AGCT 3 cut(s) 55, 103, 353
Alw21I GWGCWC 2 cut(s) 105, 355
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 1 cut(s) 219
AoxI GGCC 2 cut(s) 88, 346
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 203
AsuC2I CCSGG 2 cut(s) 24, 185
AsuHPI GGTGA 1 cut(s) 70
AvaII GGWCC 1 cut(s) 203
AxyI CCTNAGG 1 cut(s) 357
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 105, 355
BauI CACGAG 1 cut(s) 386
Bbv12I GWGCWC 2 cut(s) 105, 355
BccI CCATC 1 cut(s) 475
BceAI ACGGC 1 cut(s) 103
BcgI CGANNNNNNTGC 4 cut(s) 144, 178, 374, 408
BcnI CCSGG 2 cut(s) 24, 185
BcoDI GTCTC 1 cut(s) 174
BfmI CTRYAG 1 cut(s) 340
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
Bme1390I CCNGG 2 cut(s) 24, 185
Bme18I GGWCC 1 cut(s) 203
BmgBI CACGTC 1 cut(s) 297
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 2 cut(s) 6, 35
BmrFI CCNGG 2 cut(s) 24, 185
BmrI ACTGGG 1 cut(s) 385
BmuI ACTGGG 1 cut(s) 385
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 2 cut(s) 24, 185
Bsa29I ATCGAT 1 cut(s) 595
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 174
BsaJI CCNNGG 2 cut(s) 143, 206
BsaXI ACNNNNNCTCC 6 cut(s) 404, 407, 425, 434, 437, 455
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 43
Bse1I ACTGG 4 cut(s) 8, 280, 380, 502
Bse21I CCTNAGG 1 cut(s) 357
BseCI ATCGAT 1 cut(s) 595
BseDI CCNNGG 2 cut(s) 143, 206
BseLI CCNNNNNNNGG 2 cut(s) 30, 43
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 4 cut(s) 8, 280, 380, 502
BseRI GAGGAG 5 cut(s) 65, 345, 412, 430, 433
Bsh1236I CGCG 1 cut(s) 208
Bsh1285I CGRYCG 1 cut(s) 315
BshFI GGCC 2 cut(s) 90, 348
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 1 cut(s) 595
BsiEI CGRYCG 1 cut(s) 315
BsiHKAI GWGCWC 2 cut(s) 105, 355
BsiSI CCGG 2 cut(s) 24, 185
BslI CCNNNNNNNGG 2 cut(s) 30, 43
BsmAI GTCTC 1 cut(s) 174
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 2 cut(s) 90, 348
Bso31I GGTCTC 1 cut(s) 174
Bsp1286I GDGCHC 2 cut(s) 105, 355
Bsp143I GATC 4 cut(s) 124, 211, 312, 614
BspACI CCGC 3 cut(s) 206, 208, 453
BspANI GGCC 2 cut(s) 90, 348
BspCNI CTCAG 1 cut(s) 370
BspDI ATCGAT 1 cut(s) 595
BspFNI CGCG 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 6, 35
BspPI GGATC 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 174
BsrI ACTGG 4 cut(s) 8, 280, 380, 502
BssECI CCNNGG 2 cut(s) 143, 206
BssMI GATC 4 cut(s) 124, 211, 312, 614
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 386
BssT1I CCWWGG 1 cut(s) 143
Bst2BI CACGAG 1 cut(s) 386
Bst4CI ACNGT 2 cut(s) 491, 568
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 2 cut(s) 140, 496
BstDEI CTNAG 1 cut(s) 357
BstDSI CCRYGG 1 cut(s) 206
BstFNI CGCG 1 cut(s) 208
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 4 cut(s) 127, 214, 315, 617
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 4 cut(s) 124, 211, 312, 614
BstMCI CGRYCG 1 cut(s) 315
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstSCI CCNGG 2 cut(s) 22, 183
BstSFI CTRYAG 1 cut(s) 340
BstUI CGCG 1 cut(s) 208
Bsu15I ATCGAT 1 cut(s) 595
Bsu36I CCTNAGG 1 cut(s) 357
BsuRI GGCC 2 cut(s) 90, 348
BsuTUI ATCGAT 1 cut(s) 595
BtgI CCRYGG 1 cut(s) 206
BtrI CACGTC 1 cut(s) 297
BtsIMutI CAGTG 1 cut(s) 15
Cac8I GCNNGC 2 cut(s) 140, 496
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 203
Cfr42I CCGCGG 1 cut(s) 209
ClaI ATCGAT 1 cut(s) 595
Csp6I GTAC 1 cut(s) 193
CviAII CATG 1 cut(s) 382
CviJI RGCY 7 cut(s) 55, 90, 103, 142, 231, 348, 353
CviKI_1 RGCY 7 cut(s) 55, 90, 103, 142, 231, 348, 353
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 1 cut(s) 357
DinI GGCGCC 1 cut(s) 6
DpnI GATC 4 cut(s) 126, 213, 314, 616
DpnII GATC 4 cut(s) 124, 211, 312, 614
EaeI YGGCCR 1 cut(s) 88
Ecl136II GAGCTC 2 cut(s) 103, 353
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 2 cut(s) 105, 355
Eco31I GGTCTC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 203
Eco53kI GAGCTC 2 cut(s) 103, 353
Eco57I CTGAAG 2 cut(s) 138, 217
Eco81I CCTNAGG 1 cut(s) 357
EcoICRI GAGCTC 2 cut(s) 103, 353
EcoT14I CCWWGG 1 cut(s) 143
EcoT38I GRGCYC 2 cut(s) 105, 355
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 143
FaeI CATG 1 cut(s) 385
FaiI YATR 1 cut(s) 383
FatI CATG 1 cut(s) 381
FauI CCCGC 1 cut(s) 201
FblI GTMKAC 1 cut(s) 272
Fnu4HI GCNGC 1 cut(s) 453
FriOI GRGCYC 2 cut(s) 105, 355
Fsp4HI GCNGC 1 cut(s) 453
GlaI GCGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 453
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 90, 348
HapII CCGG 2 cut(s) 24, 185
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 385
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HinfI GANTC 1 cut(s) 304
HpaII CCGG 2 cut(s) 24, 185
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 3 cut(s) 273, 337, 564
Hpy188I TCNGA 6 cut(s) 236, 317, 369, 427, 462, 476
Hpy188III TCNNGA 3 cut(s) 73, 116, 546
Hpy8I GTNNAC 3 cut(s) 273, 337, 564
Hpy99I CGWCG 2 cut(s) 46, 404
HpyCH4III ACNGT 2 cut(s) 491, 568
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 1 cut(s) 385
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 1 cut(s) 296
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 385
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KspI CCGCGG 1 cut(s) 209
Kzo9I GATC 4 cut(s) 124, 211, 312, 614
LmnI GCTCC 3 cut(s) 52, 358, 425
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 1 cut(s) 292
MalI GATC 4 cut(s) 126, 213, 314, 616
MboI GATC 4 cut(s) 124, 211, 312, 614
MboII GAAGA 2 cut(s) 334, 548
MhlI GDGCHC 2 cut(s) 105, 355
MluCI AATT 3 cut(s) 61, 149, 456
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 450
MseI TTAA 1 cut(s) 579
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 2 cut(s) 24, 185
MspR9I CCNGG 2 cut(s) 24, 185
Mva1269I GAATGC 1 cut(s) 363
MvnI CGCG 1 cut(s) 208
MwoI GCNNNNNNNGC 1 cut(s) 254
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 2 cut(s) 24, 185
NdeII GATC 4 cut(s) 124, 211, 312, 614
NlaIII CATG 1 cut(s) 385
NlaIV GGNNCC 2 cut(s) 6, 35
NmeAIII GCCGAG 1 cut(s) 374
NmuCI GTSAC 1 cut(s) 292
PctI GAATGC 1 cut(s) 363
PfeI GAWTC 1 cut(s) 304
PflFI GACNNNGTC 1 cut(s) 377
PkrI GCNGC 1 cut(s) 454
Ple19I CGATCG 1 cut(s) 315
PluTI GGCGCC 1 cut(s) 8
Psp124BI GAGCTC 2 cut(s) 105, 355
PspN4I GGNNCC 2 cut(s) 6, 35
PspPI GGNCC 1 cut(s) 203
PsyI GACNNNGTC 1 cut(s) 377
PvuI CGATCG 1 cut(s) 315
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SacI GAGCTC 2 cut(s) 105, 355
SacII CCGCGG 1 cut(s) 209
SalI GTCGAC 1 cut(s) 271
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 4 cut(s) 124, 211, 312, 614
Sau96I GGNCC 1 cut(s) 203
ScrFI CCNGG 2 cut(s) 24, 185
SduI GDGCHC 2 cut(s) 105, 355
SetI ASST 7 cut(s) 22, 35, 57, 105, 299, 355, 440
SfcI CTRYAG 1 cut(s) 340
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 209
SgrBI CCGCGG 1 cut(s) 209
SinI GGWCC 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 3 cut(s) 61, 149, 456
SsiI CCGC 3 cut(s) 206, 208, 453
SspDI GGCGCC 1 cut(s) 4
SstI GAGCTC 2 cut(s) 105, 355
StyD4I CCNGG 2 cut(s) 22, 183
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 2 cut(s) 491, 568
TaiI ACGT 1 cut(s) 299
TaqI TCGA 6 cut(s) 105, 214, 272, 307, 399, 595
TasI AATT 3 cut(s) 61, 149, 456
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 1 cut(s) 304
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TscAI CASTG 1 cut(s) 15
TseFI GTSAC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 2 cut(s) 335, 458
TspGWI ACGGA 1 cut(s) 59
TspRI CASTG 1 cut(s) 15
Tth111I GACNNNGTC 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.