Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0015430

Physical Location & Seq

Genomic Coordinates Reverse (-)

7331790 .. 7336903

Gene Structure 5114 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1077100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3804 bp

ATGGGGAGTGTCAATGACGACAAGCTCAGATTTTGCATTGACAGAGGGGGAACCTTCACTGATGTCTATGCTGAGATCCCAGGTCAGCCTGATGGACGGGTTTTAAAACTTTTGTCTGTAGATCCATCAAACTACGACGATGCTCCTGTCGAAGGAATTCGGAGGATTCTTGAAGAGTTTACGGGGAAGAAAATTCCCCGTGCTTCAAAAATTCCCACTGATAAGATAGAGTGGATAAGAATGGGTACGACTGTAGCAACTAATGCACTTTTGGAAAGAAAAGGGGAAAGGATTGCTCTTTGTGTGACTCGAGGTTTTCGTGATTTGCTTCAGATTGGCAACCAGGCTCGTCCCAAGATATTTGACCTCACTGTATCAAAACCATCAAACCTTTATGAGGAGGTTATTGAAGTTGATGAGAGAGTTGAGCTTGCTCGTGATAATGAAGATTCTTCTTCTTCACCATCATTAGTTAAGGGGATTTCTGGTGAGATGGTTAGAGTTGTAAAGCCTATTGATGTAGAAACTTTGAAACCGTTACTCCAAGGTTTGTTGAAGAAGGGCATTAGCTGTTTGGCTGTTGTGTTAATGCATTCATATACTTACCCACAACATGAAGTAGCGGTTGCAAGCCTAGCGGAGAATTTGGGCTTCAGACATGTGTCCTTATCTTCAGCGTTGACACCCATGGTTCGAGCTGTTCCTCGGGGTCTAACAGCTAGTGTTGATGCATATTTGACGCCAGTAATTAAAGAGTATCTAACAGGGTTTATGTCAAAATTTGATGAAGGGGTGGAGAAGGTGAATGTTTTGTTTATGCAATCTGATGGTGGACTTGCACCAGAAAGTCGATTTTCAGGGCATAAAGCTGTTTTATCTGGTCCTGCTGGTGGGGTTGTGGGTTACTCACAAACACTTTTTGGGCTTGAAACTGAGAAACCTCTCATTGGGTTTGACATGGGTGGCACATCTACAGATGTGAGCCGTTATGCTGGGACTTATGAACAAGTCCTGGAAACCCAGATTGCAGGTACCATAATACAAGCACCTCAGCTTGACATAAGTACAGTAGCTGCTGGTGGTGGATCAAAGTTGAAGTTCCAGTTTGGATCTTTCCGGGTAGGACCGGAATCAGTGGGTGCACATCCTGGTCCAGTATGTTACCGGAAAGGTGGGGAGTTGGCAGTTACAGATGCAAACTTAGTTCTCGGATATGTAATTCCTGATTATTTCCCACCAATATTTGGGCTCAATGAAGATGAGCCTCTAGATATCAAGGCAACAAGAGACAAGTTTGCAAACCTTGCAAGCCAAATAAATTCCTACCGGAAAAGCCAGGATCCATCTGCAAAGGACATGACAGTGGAGGACATTGCACTGGGATTTGTAAATGTTGCCAATGAGACTATGTGCCGTCCGATAAGGCAGTTAACGGAGATGAAGGGCCATGAAACAAGGAACCATGCCCTTGCTTGCTTTGGAGGTGCTGGGCCTCAGCATGCCTGTGCAATTGCTAGATCATTGGGTATGAAAGAAGTACTTATACACAGATTTTGTGGAATCTTAAGTGCTTATGGGATGGGATTGGCAGATGTTGTAGAAGACGCACAGGAGCCTTACTCTGCAGTTTACAGTCTTAAATCTGTCGAGGAAGCCTCTCACAGGGAAGCTACTTTATTGAATCAAGTAAAGAAGAAGCTGCACGATCAAGGTTTTAAAGATGAAAACATGACAACAGAGACATATCTGAATTTGAGATATGAAGGTACGGACACCTCAATCATGGTTAAGAAACGAATTTCTGAAGATGGGAGAGGATTTAACTATGATATTGACTTTGTGGAACTATTCCAGCAGGAGTATGGTTTTAAATTACTGAATAGGAATATCCTAAAATGTGATGTTAGGGTTCGTGGGGTAGGTGTCACTAATATATTGAAGCCCCTGCCTGTGGAATCTACCTCATGTTCTCCTAAAGTTGAAGGTAATTACAAGGTTTATTTTGGGAGTGGGTGGCAGGAAACACCTCTATACAAGCTTGAGAAACTCGGGTATGGGCATATCTTGCCTGGCCCGGCAATAATCATGAATGGGAATAGTACTGTAATAGTAGAACCTAAATGTAAAGCTATAATAACCAAATATGGTAACATTAGAATTGAAATTGACTCTACTCCAAGCACCATGAAAGTAGCGGACAAAGTTGCTAATGTTGTGCAGCTCTCAATCTTCAATCATAGATTTATGGGTATAGCTGAACAGATGGGAAGGACCCTGCAGAGAACTTCCATATCAACCAATATCAAGGAGCGGCTAGATTTCTCTTGTGCCCTCTTTGGACCTGATGGGGGACTAGTTGCCAATGCCCCTCATGTCCCTGTGCATTTAGGAGCAATGTCCAGTACTGTCCGGTGGCAGATCAATTACTGGGGTGACAACTTGGATGAAGGAGATGTTTTGGTCACCAATCATCCTTGTGCCGGAGGTAGTCATCTTCCTGATATAACTGTTATTACCCCTGTATTTGATAATGGAAGGCTGGTGTTTTTTGTGGCGAGTAGAGGGCATCATGCAGAGATTGGGGGTATTACTCCTGGAAGCATGCCACCTTTTTCCAAATCTATATGGGAAGAAGGGGCTGCCATAAAAGCATTCAAGCTTGTGGAAAAGGGAATTTTTCAAGAAGAAGGGATCATTCGACTTCTTCGATTCCCCTGTTCTGATGAATTGGTTCAAAAGATCCCAGGCACTCGCAGGATTCAAGATAATCTGTCAGATCTTCGAGCTCAAGTAGCTGCGAACCAGAGGGGAATTACACTTATCAAAGAGCTTATTGAGCAGTATGGTTTGGACACTGTTCAGGCTTACATGACCTATGTACAGCTAAATGCAGAAGAAGCAGTGAGAGAAATGCTTAAGTCTGTTGCTGCTAGAGTTTTATCTCAGTCGGCTAGTTCTGGAGATCAGAGTTCTGTTACTATTGAAGAAGAGGATTACATGGATGATGGATCCGTTATTCATTTGAAACTTACAATTGATTCTGTTAAAGGGGAAGCAAATTTTGATTTCAGTGGAACCAGTCCGGAAGTATATGGGAATTGGAATGCTCCAGAAGCCGTAACTGCTGCAGCAGTTATATACTGTCTTCGCTGCTTGGTGGATGTTGACATTCCTCTAAATCAAGGTTGTTTGGCTCCTGTGAAAATCTATATTCCACCAGGCTCTTTCCTTTCTCCAAGTGATAAGGCTGCTGTCGTGGGTGGTAACGTTCTCACTTCTCAGAGAATAACTGACGTTGTGCTTACTGCATTCCAAGCTTGTGCTTGTTCACAGGGTTGTATGAATAATCTCACTTTTGGGGATAATACATTTGGTTATTATGAAACAATCGGAGGTGGGAGTGGAGCTGGTCCAACTTGGGATGGGACGAGTGGAGTGCAGTGTCATATGACCAATACCCGAATGACTGATCCAGAAATTTTTGAACAGAGATATCCTGTTCTTTTGCATAAATTTGGCCTGAGAGAGAACAGCGGTGGAGTTGGGTATCATAGAGGGGGTGATGGGCTTGTAAGGGAGATAGAGTTCAAGCGCCCAATTGTTGTGAGCATTCTTTCAGAGAGGCGTGTTCACGCACCAAGGGGGTTGAAAGGAGGGAAAGATGGTGCTCGGGGGGCTAACTTCCTAATCACAAAAGATAAGCGAAGGGTTTATCTTGGCGGGAAGAACACGGTTGAAGTGCAGTCAGGGGAAATTCTTCAGATTTTAACTCCTGGTGGTGGTGGATGGGGCTCTCCTCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006518 GO:0006575 GO:0006749 GO:0006751 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009506 GO:0009987 GO:0016787 GO:0016810 GO:0016812 GO:0017168 GO:0030054 GO:0034641 GO:0042219 GO:0043171 GO:0043603 GO:0044237 GO:0044248 GO:0044273 GO:0044424 GO:0044444 GO:0044464 GO:0051186 GO:0051187 GO:0055044 GO:0071704 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00480 map00480

Pfam Domains

Protein Families

Hydant_A_N Hydantoinase_A Hydantoinase_B

Protein Analysis

1268

Amino Acids

138.1

Weight (kDa)

5.98

Isoelectric Point (pI)

36.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydant_A_N	PF05378	10 - 216	1.4e-58	Hydantoinase/oxoprolinase N-terminal region
Hydantoinase_A	PF01968	236 - 535	1.4e-104	Hydantoinase/oxoprolinase
Hydant_A_C	PF19278	558 - 714	3.7e-07	Hydantoinase/oxoprolinase C-terminal domain
Hydantoinase_B	PF02538	739 - 1266	5.8e-215	Hydantoinase B/oxoprolinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015430)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37830
fragaria_vesca	FvH4_7g07120 FvH4_7g07120
malus_domestica	MD07G1077100.v1.1
prunus_persica	Prupe.2G098100_v2.0.a1
pyrus_communis	pycom07g06060
rosa_chinensis	RchiOBHm_Chr1g0337071
rosa_laevigata	RLG00000029307
rosa_multiflora	Rmu_sc0010164.1_g000003
rosa_roxburghii	Rroxscaffold_4G00314980
rosa_rugosa	Rorug01G0130900
rosa_samantha	Rh1AG151600 Rh1BG120200 Rh1CG142800 Rh1DG158800
rosa_wichuraiana	Rw1G012400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1019
Acc65I	GGTACC	1 cut(s)	1031
AccB1I	GGYRCC	1 cut(s)	1031
AccB7I	CCANNNNNTGG	1 cut(s)	1244
AccBSI	CCGCTC	1 cut(s)	2311
AccIII	TCCGGA	1 cut(s)	3082
AciI	CCGC	6 cut(s)	623, 638, 2195, 2311, 3534, 3720
AclI	AACGTT	1 cut(s)	3267
AcuI	CTGAAG	5 cut(s)	314, 637, 657, 1824, 3743
AcyI	GRCGYC	1 cut(s)	740
AfaI	GTAC	8 cut(s)	247, 1033, 1066, 1539, 1768, 2101, 2404, 2880
AflII	CTTAAG	2 cut(s)	1564, 2915
AflIII	ACRYGT	1 cut(s)	658
AhlI	ACTAGT	1 cut(s)	2353
AloI	GAACNNNNNNTCC	2 cut(s)	3569, 3601
Alw21I	GWGCWC	3 cut(s)	1144, 2788, 3670
Alw26I	GTCTC	3 cut(s)	1281, 1397, 1733
Alw44I	GTGCAC	1 cut(s)	1140
AlwNI	CAGNNNCTG	1 cut(s)	1073
Ama87I	CYCGRG	4 cut(s)	309, 705, 2048, 3669
Aor13HI	TCCGGA	1 cut(s)	3082
AoxI	GGCC	4 cut(s)	1444, 1490, 2071, 3517
ApaLI	GTGCAC	1 cut(s)	1140
Asp700I	GAANNNNTTC	3 cut(s)	156, 2730, 3756
Asp718I	GGTACC	1 cut(s)	1031
AspLEI	GCGC	1 cut(s)	3594
AspS9I	GGNCC	9 cut(s)	881, 1124, 1151, 1444, 1490, 2072, 2271, 2339, 3410
AsuC2I	CCSGG	2 cut(s)	1118, 2075
AsuHPI	GGTGA	6 cut(s)	453, 500, 814, 2444, 2455, 3572
AvaI	CYCGRG	4 cut(s)	309, 705, 2048, 3669
AvaII	GGWCC	6 cut(s)	881, 1124, 1151, 2271, 2339, 3410
BaeGI	GKGCMC	2 cut(s)	1144, 2332
BaeI	ACNNNNGTAYC	2 cut(s)	237, 270
BamHI	GGATCC	2 cut(s)	1339, 3008
BanI	GGYRCC	1 cut(s)	1031
BanII	GRGCYC	3 cut(s)	1251, 2788, 3794
BarI	GAAGNNNNNNTAC	2 cut(s)	1527, 1559
BauI	CACGAG	1 cut(s)	435
BbsI	GAAGAC	2 cut(s)	1608, 3137
Bbv12I	GWGCWC	3 cut(s)	1144, 2788, 3670
BbvCI	CCTCAGC	2 cut(s)	1050, 1494
BceAI	ACGGC	3 cut(s)	969, 1398, 3101
BcgI	CGANNNNNNTGC	2 cut(s)	3418, 3452
BcnI	CCSGG	2 cut(s)	1118, 2075
BcoDI	GTCTC	3 cut(s)	1281, 1397, 1733
BcuI	ACTAGT	1 cut(s)	2353
BfaI	CTAG	8 cut(s)	635, 720, 1268, 1515, 2315, 2354, 2931, 2952
BfmI	CTRYAG	6 cut(s)	117, 252, 972, 1623, 2276, 3126
BfoI	RGCGCY	1 cut(s)	3595
BfrI	CTTAAG	2 cut(s)	1564, 2915
BfuAI	ACCTGC	1 cut(s)	1019
BglII	AGATCT	1 cut(s)	2776
BmcAI	AGTACT	3 cut(s)	1539, 2101, 2404
Bme18I	GGWCC	6 cut(s)	881, 1124, 1151, 2271, 2339, 3410
BmeT110I	CYCGRG	4 cut(s)	309, 705, 2048, 3669
BmgT120I	GGNCC	9 cut(s)	881, 1124, 1151, 1444, 1490, 2072, 2271, 2339, 3410
BmiI	GGNNCC	9 cut(s)	52, 1033, 1341, 1460, 1614, 2273, 3010, 3076, 3195
BmrI	ACTGGG	2 cut(s)	1388, 2437
BmsI	GCATC	4 cut(s)	130, 718, 1183, 2575
BmuI	ACTGGG	2 cut(s)	1388, 2437
BoxI	GACNNNNGTC	1 cut(s)	661
BpiI	GAAGAC	2 cut(s)	1608, 3137
BplI	GAGNNNNNCTC	4 cut(s)	1604, 1636, 1640, 1672
BpmI	CTGGAG	2 cut(s)	2979, 3093
Bpu10I	CCTNAGC	2 cut(s)	1050, 1494
BpuEI	CTTGAG	2 cut(s)	2060, 2772
BpuMI	CCSGG	2 cut(s)	1118, 2075
BsaHI	GRCGYC	1 cut(s)	740
BsaJI	CCNNGG	6 cut(s)	79, 544, 687, 704, 2743, 3638
BsaWI	WCCGGW	5 cut(s)	1126, 1164, 1326, 2409, 3082
BsaXI	ACNNNNNCTCC	4 cut(s)	525, 555, 3569, 3599
Bse1I	ACTGG	7 cut(s)	743, 1102, 1154, 1383, 2400, 2432, 3078
Bse3DI	GCAATG	2 cut(s)	1371, 2400
BseAI	TCCGGA	1 cut(s)	3082
BseDI	CCNNGG	6 cut(s)	79, 544, 687, 704, 2743, 3638
BseGI	GGATG	8 cut(s)	1144, 1584, 2449, 2470, 3007, 3166, 3427, 3791
BseMI	GCAATG	2 cut(s)	1371, 2400
BseMII	CTCAG	8 cut(s)	40, 63, 924, 1064, 1508, 2957, 3293, 3512
BseNI	ACTGG	7 cut(s)	743, 1102, 1154, 1383, 2400, 2432, 3078
BseRI	GAGGAG	2 cut(s)	413, 3786
BseSI	GKGCMC	2 cut(s)	1144, 2332
BseYI	CCCAGC	2 cut(s)	992, 1487
BsgI	GTGCAG	4 cut(s)	1685, 2237, 3458, 3761
BshFI	GGCC	4 cut(s)	1446, 1492, 2073, 3519
BshNI	GGYRCC	1 cut(s)	1031
BsiHKAI	GWGCWC	3 cut(s)	1144, 2788, 3670
BsiHKCI	CYCGRG	4 cut(s)	309, 705, 2048, 3669
BsiSI	CCGG	8 cut(s)	1117, 1127, 1165, 1327, 2075, 2410, 2481, 3083
BslFI	GGGAC	5 cut(s)	336, 1009, 2360, 2364, 3439
BsmAI	GTCTC	3 cut(s)	1281, 1397, 1733
BsmFI	GGGAC	5 cut(s)	336, 1009, 2360, 2364, 3439
BsmI	GAATGC	5 cut(s)	592, 2651, 3109, 3308, 3609
BsnI	GGCC	4 cut(s)	1446, 1492, 2073, 3519
BsoBI	CYCGRG	4 cut(s)	309, 705, 2048, 3669
Bsp1286I	GDGCHC	6 cut(s)	1144, 1251, 2332, 2788, 3670, 3794
Bsp13I	TCCGGA	1 cut(s)	3082
Bsp1407I	TGTACA	1 cut(s)	2878
Bsp19I	CCATGG	1 cut(s)	687
BspACI	CCGC	6 cut(s)	623, 638, 2195, 2311, 3534, 3720
BspANI	GGCC	4 cut(s)	1446, 1492, 2073, 3519
BspCNI	CTCAG	8 cut(s)	39, 64, 925, 1063, 1507, 2956, 3292, 3513
BspEI	TCCGGA	1 cut(s)	3082
BspHI	TCATGA	1 cut(s)	2085
BspLI	GGNNCC	9 cut(s)	52, 1033, 1341, 1460, 1614, 2273, 3010, 3076, 3195
BspMAI	CTGCAG	3 cut(s)	1627, 2280, 3130
BspMI	ACCTGC	1 cut(s)	1019
BspT107I	GGYRCC	1 cut(s)	1031
BspTI	CTTAAG	2 cut(s)	1564, 2915
BsrBI	CCGCTC	1 cut(s)	2311
BsrDI	GCAATG	2 cut(s)	1371, 2400
BsrGI	TGTACA	1 cut(s)	2878
BsrI	ACTGG	7 cut(s)	743, 1102, 1154, 1383, 2400, 2432, 3078
BssECI	CCNNGG	6 cut(s)	79, 544, 687, 704, 2743, 3638
BssNI	GRCGYC	1 cut(s)	740
BssSI	CACGAG	1 cut(s)	435
BssT1I	CCWWGG	3 cut(s)	544, 687, 3638
Bst2BI	CACGAG	1 cut(s)	435
Bst6I	CTCTTC	2 cut(s)	168, 2982
BstACI	GRCGYC	1 cut(s)	740
BstAFI	CTTAAG	2 cut(s)	1564, 2915
BstAPI	GCANNNNNTGC	3 cut(s)	263, 1304, 2065
BstAUI	TGTACA	1 cut(s)	2878
BstC8I	GCNNGC	6 cut(s)	432, 631, 1309, 1474, 1500, 2603
BstDEI	CTNAG	9 cut(s)	26, 72, 933, 1050, 1201, 1494, 2943, 3279, 3521
BstDSI	CCRYGG	1 cut(s)	687
BstEII	GGTNACC	1 cut(s)	2461
BstENI	CCTNNNNNAGG	3 cut(s)	150, 395, 1660
BstF5I	GGATG	8 cut(s)	1144, 1584, 2449, 2470, 3007, 3166, 3427, 3791
BstH2I	RGCGCY	1 cut(s)	3595
BstHHI	GCGC	1 cut(s)	3594
BstMAI	GTCTC	3 cut(s)	1281, 1397, 1733
BstNSI	RCATGY	3 cut(s)	662, 1502, 2605
BstPAI	GACNNNNGTC	1 cut(s)	661
BstPI	GGTNACC	1 cut(s)	2461
BstSFI	CTRYAG	6 cut(s)	117, 252, 972, 1623, 2276, 3126
BstSLI	GKGCMC	2 cut(s)	1144, 2332
BstV2I	GAAGAC	2 cut(s)	1608, 3137
BstX2I	RGATCY	7 cut(s)	75, 121, 1109, 1339, 2739, 2776, 3008
BstYI	RGATCY	7 cut(s)	75, 121, 1109, 1339, 2739, 2776, 3008
BsuRI	GGCC	4 cut(s)	1446, 1492, 2073, 3519
BtgI	CCRYGG	1 cut(s)	687
BtsCI	GGATG	8 cut(s)	1144, 1584, 2449, 2470, 3007, 3166, 3427, 3791
BtsI	GCAGTG	2 cut(s)	2907, 3446
BveI	ACCTGC	1 cut(s)	1019
Cac8I	GCNNGC	6 cut(s)	432, 631, 1309, 1474, 1500, 2603
CaiI	CAGNNNCTG	1 cut(s)	1073
CciI	TCATGA	1 cut(s)	2085
CfoI	GCGC	1 cut(s)	3594
Cfr13I	GGNCC	9 cut(s)	881, 1124, 1151, 1444, 1490, 2072, 2271, 2339, 3410
CseI	GACGC	2 cut(s)	748, 1613
Csp6I	GTAC	8 cut(s)	246, 1032, 1065, 1538, 1767, 2100, 2403, 2879
CviQI	GTAC	8 cut(s)	246, 1032, 1065, 1538, 1767, 2100, 2403, 2879
DdeI	CTNAG	9 cut(s)	26, 72, 933, 1050, 1201, 1494, 2943, 3279, 3521
DraI	TTTAAA	3 cut(s)	105, 1717, 1870
Eam1104I	CTCTTC	2 cut(s)	168, 2982
EarI	CTCTTC	2 cut(s)	168, 2982
Ecl136II	GAGCTC	1 cut(s)	2786
Eco130I	CCWWGG	3 cut(s)	544, 687, 3638
Eco24I	GRGCYC	3 cut(s)	1251, 2788, 3794
Eco32I	GATATC	2 cut(s)	1273, 3494
Eco47I	GGWCC	6 cut(s)	881, 1124, 1151, 2271, 2339, 3410
Eco53kI	GAGCTC	1 cut(s)	2786
Eco57I	CTGAAG	5 cut(s)	314, 637, 657, 1824, 3743
Eco88I	CYCGRG	4 cut(s)	309, 705, 2048, 3669
Eco91I	GGTNACC	1 cut(s)	2461
EcoICRI	GAGCTC	1 cut(s)	2786
EcoNI	CCTNNNNNAGG	3 cut(s)	150, 395, 1660
EcoO109I	RGGNCCY	1 cut(s)	2271
EcoO65I	GGTNACC	1 cut(s)	2461
EcoRI	GAATTC	1 cut(s)	156
EcoRV	GATATC	2 cut(s)	1273, 3494
EcoT14I	CCWWGG	3 cut(s)	544, 687, 3638
EcoT22I	ATGCAT	2 cut(s)	594, 733
EcoT38I	GRGCYC	3 cut(s)	1251, 2788, 3794
ErhI	CCWWGG	3 cut(s)	544, 687, 3638
FalI	AAGNNNNNCTT	2 cut(s)	1524, 1556
FaqI	GGGAC	5 cut(s)	336, 1009, 2360, 2364, 3439
FauI	CCCGC	1 cut(s)	3713
FauNDI	CATATG	1 cut(s)	3447
FokI	GGATG	8 cut(s)	1131, 1591, 2456, 2457, 3014, 3173, 3434, 3798
FriOI	GRGCYC	3 cut(s)	1251, 2788, 3794
FspBI	CTAG	8 cut(s)	635, 720, 1268, 1515, 2315, 2354, 2931, 2952
GlaI	GCGC	1 cut(s)	3593
GsaI	CCCAGC	2 cut(s)	996, 1491
GsuI	CTGGAG	2 cut(s)	2979, 3093
HaeII	RGCGCY	1 cut(s)	3595
HaeIII	GGCC	4 cut(s)	1446, 1492, 2073, 3519
HapII	CCGG	8 cut(s)	1117, 1127, 1165, 1327, 2075, 2410, 2481, 3083
HgaI	GACGC	2 cut(s)	748, 1613
HhaI	GCGC	1 cut(s)	3594
Hin1I	GRCGYC	1 cut(s)	740
Hin6I	GCGC	1 cut(s)	3592
HinP1I	GCGC	1 cut(s)	3592
HincII	GTYRAC	3 cut(s)	681, 1431, 3166
HindII	GTYRAC	3 cut(s)	681, 1431, 3166
HindIII	AAGCTT	3 cut(s)	2036, 2657, 3315
HpaI	GTTAAC	1 cut(s)	1431
HpaII	CCGG	8 cut(s)	1117, 1127, 1165, 1327, 2075, 2410, 2481, 3083
HphI	GGTGA	6 cut(s)	453, 500, 814, 2444, 2455, 3572
Hpy166II	GTNNAC	9 cut(s)	180, 681, 833, 1142, 1431, 1630, 3166, 3329, 3631
Hpy8I	GTNNAC	9 cut(s)	180, 681, 833, 1142, 1431, 1630, 3166, 3329, 3631
Hpy99I	CGWCG	1 cut(s)	140
HpyCH4IV	ACGT	2 cut(s)	3267, 3294
HpyF3I	CTNAG	9 cut(s)	26, 72, 933, 1050, 1201, 1494, 2943, 3279, 3521
HpySE526I	ACGT	2 cut(s)	3267, 3294
Hsp92I	GRCGYC	1 cut(s)	740
HspAI	GCGC	1 cut(s)	3592
Kpn2I	TCCGGA	1 cut(s)	3082
KpnI	GGTACC	1 cut(s)	1035
KspAI	GTTAAC	1 cut(s)	1431
LmnI	GCTCC	7 cut(s)	148, 1612, 2308, 2390, 3112, 3199, 3404
LweI	GCATC	4 cut(s)	130, 718, 1183, 2575
MaeI	CTAG	8 cut(s)	635, 720, 1268, 1515, 2315, 2354, 2931, 2952
MaeII	ACGT	2 cut(s)	3267, 3294
MbiI	CCGCTC	1 cut(s)	2311
MfeI	CAATTG	3 cut(s)	1509, 3033, 3597
MflI	RGATCY	7 cut(s)	75, 121, 1109, 1339, 2739, 2776, 3008
MhlI	GDGCHC	6 cut(s)	1144, 1251, 2332, 2788, 3670, 3794
MlyI	GAGTC	2 cut(s)	301, 2162
MmeI	TCCRAC	1 cut(s)	3437
Mph1103I	ATGCAT	2 cut(s)	594, 733
MroI	TCCGGA	1 cut(s)	3082
MroXI	GAANNNNTTC	3 cut(s)	156, 2730, 3756
MslI	CAYNNNNRTG	3 cut(s)	1361, 1503, 2475
MspA1I	CMGCKG	1 cut(s)	3534
MspCI	CTTAAG	2 cut(s)	1564, 2915
MspI	CCGG	8 cut(s)	1117, 1127, 1165, 1327, 2075, 2410, 2481, 3083
MunI	CAATTG	3 cut(s)	1509, 3033, 3597
Mva1269I	GAATGC	5 cut(s)	592, 2651, 3109, 3308, 3609
NciI	CCSGG	2 cut(s)	1118, 2075
NcoI	CCATGG	1 cut(s)	687
NdeI	CATATG	1 cut(s)	3447
NlaIV	GGNNCC	9 cut(s)	52, 1033, 1341, 1460, 1614, 2273, 3010, 3076, 3195
NmuCI	GTSAC	4 cut(s)	304, 1924, 2432, 2461
NsiI	ATGCAT	2 cut(s)	594, 733
NspI	RCATGY	3 cut(s)	662, 1502, 2605
PaeI	GCATGC	2 cut(s)	1502, 2605
PaeR7I	CTCGAG	1 cut(s)	309
PagI	TCATGA	1 cut(s)	2085
PciI	ACATGT	1 cut(s)	658
PcsI	WCGNNNNNNNCGW	2 cut(s)	316, 2704
PctI	GAATGC	5 cut(s)	592, 2651, 3109, 3308, 3609
PdmI	GAANNNNTTC	3 cut(s)	156, 2730, 3756
PfeI	GAWTC	9 cut(s)	166, 449, 1130, 1560, 1681, 1955, 2709, 2758, 3038
PflMI	CCANNNNNTGG	1 cut(s)	1244
PfoI	TCCNGGA	2 cut(s)	1011, 2593
PleI	GAGTC	2 cut(s)	301, 2162
PpsI	GAGTC	2 cut(s)	301, 2162
PpuMI	RGGWCCY	1 cut(s)	2271
PscI	ACATGT	1 cut(s)	658
PshAI	GACNNNNGTC	1 cut(s)	661
Psp124BI	GAGCTC	1 cut(s)	2788
Psp1406I	AACGTT	1 cut(s)	3267
Psp5II	RGGWCCY	1 cut(s)	2271
PspEI	GGTNACC	1 cut(s)	2461
PspFI	CCCAGC	2 cut(s)	992, 1487
PspN4I	GGNNCC	9 cut(s)	52, 1033, 1341, 1460, 1614, 2273, 3010, 3076, 3195
PspPI	GGNCC	9 cut(s)	881, 1124, 1151, 1444, 1490, 2072, 2271, 2339, 3410
PspPPI	RGGWCCY	1 cut(s)	2271
PspXI	VCTCGAGB	1 cut(s)	309
PstI	CTGCAG	3 cut(s)	1627, 2280, 3130
PstNI	CAGNNNCTG	1 cut(s)	1073
PsuI	RGATCY	7 cut(s)	75, 121, 1109, 1339, 2739, 2776, 3008
RsaI	GTAC	8 cut(s)	247, 1033, 1066, 1539, 1768, 2101, 2404, 2880
RsaNI	GTAC	8 cut(s)	246, 1032, 1065, 1538, 1767, 2100, 2403, 2879
RseI	CAYNNNNRTG	3 cut(s)	1361, 1503, 2475
SacI	GAGCTC	1 cut(s)	2788
Sau96I	GGNCC	9 cut(s)	881, 1124, 1151, 1444, 1490, 2072, 2271, 2339, 3410
ScaI	AGTACT	3 cut(s)	1539, 2101, 2404
SchI	GAGTC	2 cut(s)	301, 2162
SduI	GDGCHC	6 cut(s)	1144, 1251, 2332, 2788, 3670, 3794
SfaNI	GCATC	4 cut(s)	130, 718, 1183, 2575
SfcI	CTRYAG	6 cut(s)	117, 252, 972, 1623, 2276, 3126
Sfr274I	CTCGAG	1 cut(s)	309
SinI	GGWCC	6 cut(s)	881, 1124, 1151, 2271, 2339, 3410
SlaI	CTCGAG	1 cut(s)	309
SmiMI	CAYNNNNRTG	3 cut(s)	1361, 1503, 2475
SmlI	CTYRAG	5 cut(s)	309, 1564, 2039, 2787, 2915
SmoI	CTYRAG	5 cut(s)	309, 1564, 2039, 2787, 2915
SpeI	ACTAGT	1 cut(s)	2353
SphI	GCATGC	2 cut(s)	1502, 2605
SsiI	CCGC	6 cut(s)	623, 638, 2195, 2311, 3534, 3720
SspI	AATATT	1 cut(s)	1242
SspMI	CTAG	8 cut(s)	635, 720, 1268, 1515, 2315, 2354, 2931, 2952
SstI	GAGCTC	1 cut(s)	2788
StyI	CCWWGG	3 cut(s)	544, 687, 3638
TaiI	ACGT	2 cut(s)	3270, 3297
TaqI	TCGA	8 cut(s)	150, 310, 694, 850, 1647, 2698, 2707, 2782
TatI	WGTACW	5 cut(s)	1064, 1537, 2099, 2402, 2878
TauI	GCSGC	1 cut(s)	2314
TfiI	GAWTC	9 cut(s)	166, 449, 1130, 1560, 1681, 1955, 2709, 2758, 3038
TseFI	GTSAC	4 cut(s)	304, 1924, 2432, 2461
Tsp45I	GTSAC	4 cut(s)	304, 1924, 2432, 2461
TspGWI	ACGGA	3 cut(s)	1448, 1784, 3001
Van91I	CCANNNNNTGG	1 cut(s)	1244
Vha464I	CTTAAG	2 cut(s)	1564, 2915
VneI	GTGCAC	1 cut(s)	1140
VpaK11BI	GGWCC	6 cut(s)	881, 1124, 1151, 2271, 2339, 3410
XagI	CCTNNNNNAGG	3 cut(s)	150, 395, 1660
XbaI	TCTAGA	1 cut(s)	1267
XceI	RCATGY	3 cut(s)	662, 1502, 2605
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1859
XhoI	CTCGAG	1 cut(s)	309
XmnI	GAANNNNTTC	3 cut(s)	156, 2730, 3756
XspI	CTAG	8 cut(s)	635, 720, 1268, 1515, 2315, 2354, 2931, 2952
ZrmI	AGTACT	3 cut(s)	1539, 2101, 2404
Zsp2I	ATGCAT	2 cut(s)	594, 733

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.