Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0015430

Physical Location & Seq

Genomic Coordinates Forward (+)

20484913 .. 20488710

Gene Structure 3798 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG120200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3798 bp

ATGGGGAGTGCCAATGCCAACAAGCTCAGATTTTGCATTGACAGAGGGGGCACATTCACTGATGTTTATGCGCAGATCCCGGGTCAGCTTGATGGTCGTGTTTTGAAACTTTTATCGGTTGATCCATCAAACTATGATGATGCTCCTGTAGAAGGAATTCGGAGAATTCTTGAAGAATTTACCGGGGAGAAAATTTCCCGTCGTTCAAAGTTACCAACTGATAAGATAGAGTGGATAAGGATGGGTACAACTGTTGCTACAAATGCACTTTTGGAGAGGAAAGGGGAAAGCATTGCTCTTTGTGTGACTCGGGGTTTCCGCAACTTGCTGCAGATTGGTAACCAGGCTCGTCCAAATATTTTTGACCTCACTGTATCTAAACCTTCAAACCTTTATGAGGAGGTTATTGAGGTTGACGAGAGAGTTGAGCTCATTCATGATAATGAAGATTCTGCTTCTGCTTCTGCATCATTAGTTAAAGGGGTTTCTGGTGAGATGGTTAAAGTCGTAAAGCCTCTTGATGTTGAAACATTAAGGCCATTACTCAAAGGTTTGTTGGAGAAGGGAATTAGCTGTTTGGCAGTTGTGTTAATGCACTCGTATACTTACCCACAACATGAAATAGCTGTTGAAAGGTTAGCAGCTAGTATGGGCTTTAAACATGTTTCCTTATCTTCAGCTTTGACACCTATGGTTCGAGCTGTTCCTCGGGGTCTAACAGCTAGTGTTGATGCATATTTGACACCAGTAATCAAAGAGTACTTATCAGGGTTCATGTCTAAGTTCGATGAAGGGCTGGGTAAGGTTAATGTTTTGTTTATGCAATCTGATGGAGGACTTGCACCAGAAAGTCGATTTTCAGGGCACAAAGCTGTTTTGTCAGGTCCAGCTGGTGGGGTTGTGGGTTACTCTCAGACACTTTTTGGGCTTGAAACTGAGAAACCTCTTATTGGGTTTGACATGGGTGGCACATCTACTGATGTGAGCCGTTATGCTGGGACTTATGAACAAGTTCTGGAAACTCAAATTGCTGGTGCCATCATACAAGCACCTCAACTCGACATAAATACTGTAGCTTCTGGTGGCGGATCAAAATTAAAGTTCCAATTTGGAGCTTTTCGGGTAGGACCAGAATCAGTGGGTGCACATCCAGGTCCAGTGTGTTACCGTAAAGGTGGTGAATTAGCAGTTACAGATGCAAACTTAGTCCTCGGATATGTTATTCCTGATTATTTCCCATCAATATTTGGCCCCAACGAAGATCAGCCTTTAGATATTGAGGAAACAAGAAAAGAATTTGAAAATCTTGCTGAGCAAATAAATTCCTATCGAAAGAGCCAGGATTCATCTGCAAAGGACATGAAAGTGGAGGACATTGCACTGGGATTTGTGAATGTTGCCAATGAAACTATGTGCCGTCCAATAAGGCAATTAACTGAGATGAAGGGCCATGAAACAAAGAATCATGCACTTGCTTGCTTTGGAGGTGCTGGGCCTCAACATGCCTGTGCAATTGCTAGGTCGTTGGGCATGAAAGAAGTATTCATACACAGGTTTTGTGGAATCCTAAGTGCTTATGGGATGGGTTTGGCAGATGTTGTTGAAGAGGCACAGGAGCCATACTCTGCGGTTTACAGTCTGGATTCTGTCCAGGAGGCCTCTCACAGAGAAGCTGCTTTACTGAATCAAGTAAAGCAGAAGTTGCAAGAGCAAGGTTTTAGAGATGAAAACATGACTACAGAGACGTATTTGAATTTGAGATATGAAGGTACAGACACCTCAATCATGGTTAAGAAACGAAAAGACGGAGAATGTAAGTACGATCTGGACTTTGTGGAATTATTCCAGCAGGAGTACGGATTTAAATTACTGAATAGGAATATCCTATTAAATGATGTTAGGGTTCGCGGGGTAGGAGTGACTAATATATTGAAGCCACGGGCTCTGGAATCTGCATCTTGTTCTCCTAAAGTTGAAGGTAACTACAAGGTTTATTTTGAGAATGGGTGGCAAGAAACACCTCTATACAAGCTTGAGAATCTGGGTTATGGGCATGTCATACAGGGTCCGGCAATAATTATGAATGGGAATAGTACTGTGATAGTGGAACCTAAGTGCAAAGCTATGATAACCAAATATGGTAACATTAAAATTGAAATTGAGTCTGCTTCAAGCACCCTGAAAGTAGCAGAAAAGGTTGCAAATGTTGTGCAGCTCTCAATCTTTAATCATAGATTTATGGGTATAGCCGAACAGATGGGAAGGACCCTACAGAGAACTTCTATATCAACTAACATCAAAGAACGGCTAGATTTCTCATGTGCCCTCTTTGGACCTGACGGGGGATTAGTTGCTAATGCTCCTCATGTCCCTGTGCATTTAGGAGCAATGTCTAGTACAGTTCGGTGGCAGCTCAACCATTGGGGTGAAAATTTGAATGAAGGAGATGTTTTGGTCACCAATCATCCTTGTGCCGGAGGTAGCCATCTTCCTGATATAACTGTTATTACACCTGTATTTGACAATGGAAAGCTGGTATTTTTCGTGGCAAGTAGAGGGCATCATGCAGAGATAGGAGGTATTACTCCTGGAAGCATGCCACCATTTTCCAAGTCTATATGGCAAGAAGGGGCTGCTATAAAAGCATTTAAGCTTGTTGAAAAGGGGATTTTTCAAGAAGAAGGAATTACTAAACTTCTCTGCTTCCCCTCTTCTGATGACTTGGCACAAAAGATCCCAGGAACTCGCAGGCTTCAAGATAACTTATCAGATCTTCGGGCCCAAGTAGCTGCAAACCAGAGAGGAATTACTCTTATTAAAGAGCTTATTGAGCAGTATGGTTTGGAAATGGTTCAGGCTTACATGACCTATGTGCAGCTGAATGCAGAAGAAGCAGTGAGAGAAATGCTCAAGTCTGTCGCTGCTAGAGTTTTATCTCAGTCAACCTGGTCTGGAGAGAAGAGTTCTGTCACTATTGAAGAAGAGGATTACATGGATGATGGATCCATTATTCATCTGAAGCTTACAATTGATTCTGTTAAAGGTGAAGCAAATTTTGATTTTAGTGGAACCAGTCCTGAAGTATATGGGAATTGGAATGCTCCAGAAGCCGTAACAGCTGCAGCAGTTATATACTGTCTTCGCTGCTTGGTGGATGTTGATATACCTCTGAATCAAGGTTGTTTGGCTCCTGTGAAAATCTATATTCCACCTGGCTCGTTCCTCTCTCCAAGTGATAAGGCTGCTGTGGTGGGTGGTAACGTTCTCACTTCTCAGAGAATAACTGATGTTGTACTCACTGCATTTCAGGCTTGTGCTTGTTCACAGGGTTGTATGAATAATCTCACTTTTGGGGATGACACATTTGGTTATTATGAAACGATTGGAGGTGGGAGTGGAGCTGGTCCATCCTGGGATGGGACCAGTGGAGTCCAGTGTCATATGACCAATACCCGAATGACTGATCCAGAAATATTTGAACAGAGATATCCAGTTCTTTTGCATCAGTTTGCCCTCAGAGCGAACAGTGGAGGAGTTGGATATCATAGAGGTGGTAATGGGCTTGTGAGGGAGATAGAGTTCAAGCGCCCAGTCGTTGTGAGCATTCTTTCAGAGAGGCGTGTTCATGCTCCGAGAGGGTTGAAAGGAGGGGAGGATGGGTCTCGCGGAGCAAACTACCTAATCACAAAAGATAAAAGAAGGGTTTACCTGGGTGGTAAGAACACAGTTGAGGTGCAGTCAGGGGAAATTCTTCAGATTTTAACTCCTGGTGGTGGTGGATGGGGCTCTCCTCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006518 GO:0006575 GO:0006749 GO:0006751 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009506 GO:0009987 GO:0016787 GO:0016810 GO:0016812 GO:0017168 GO:0030054 GO:0034641 GO:0042219 GO:0043171 GO:0043603 GO:0044237 GO:0044248 GO:0044273 GO:0044424 GO:0044444 GO:0044464 GO:0051186 GO:0051187 GO:0055044 GO:0071704 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00480 map00480

Pfam Domains

Protein Families

Hydant_A_N Hydantoinase_A Hydantoinase_B

Protein Analysis

1265

Amino Acids

137.88

Weight (kDa)

5.75

Isoelectric Point (pI)

38.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydant_A_N	PF05378	10 - 217	3.9e-57	Hydantoinase/oxoprolinase N-terminal region
Hydantoinase_A	PF01968	237 - 536	1.8e-106	Hydantoinase/oxoprolinase
Hydant_A_C	PF19278	559 - 713	2.8e-09	Hydantoinase/oxoprolinase C-terminal domain
Hydantoinase_B	PF02538	737 - 1264	9.1e-215	Hydantoinase B/oxoprolinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015430)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37830
fragaria_vesca	FvH4_7g07120 FvH4_7g07120
malus_domestica	MD07G1077100.v1.1
prunus_persica	Prupe.2G098100_v2.0.a1
pyrus_communis	pycom07g06060
rosa_chinensis	RchiOBHm_Chr1g0337071
rosa_laevigata	RLG00000029307
rosa_multiflora	Rmu_sc0010164.1_g000003
rosa_roxburghii	Rroxscaffold_4G00314980
rosa_rugosa	Rorug01G0130900
rosa_samantha	Rh1AG151600 Rh1BG120200 Rh1CG142800 Rh1DG158800
rosa_wichuraiana	Rw1G012400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	72
AccB1I	GGYRCC	1 cut(s)	1034
AccB7I	CCANNNNNTGG	1 cut(s)	893
AccI	GTMKAC	1 cut(s)	602
AccII	CGCG	2 cut(s)	1908, 3666
AciI	CCGC	5 cut(s)	319, 1086, 1628, 1908, 3666
AclI	AACGTT	1 cut(s)	3261
AclWI	GGATC	7 cut(s)	70, 116, 1096, 2728, 2997, 3010, 3458
AcuI	CTGAAG	4 cut(s)	660, 3038, 3099, 3737
AfaI	GTAC	8 cut(s)	247, 761, 1771, 1820, 1856, 2095, 2398, 3294
AfiI	CCNNNNNNNGG	8 cut(s)	152, 397, 893, 950, 2342, 2474, 3417, 3773
AflIII	ACRYGT	1 cut(s)	661
AloI	GAACNNNNNNTCC	2 cut(s)	3563, 3595
Alw21I	GWGCWC	2 cut(s)	432, 1147
Alw26I	GTCTC	2 cut(s)	1736, 3666
Alw44I	GTGCAC	1 cut(s)	1143
AlwI	GGATC	7 cut(s)	70, 116, 1096, 2728, 2997, 3010, 3458
Ama87I	CYCGRG	3 cut(s)	79, 309, 708
AoxI	GGCC	6 cut(s)	536, 1249, 1447, 1493, 1656, 2778
ApaI	GGGCCC	1 cut(s)	2782
ApaLI	GTGCAC	1 cut(s)	1143
Asp700I	GAANNNNTTC	4 cut(s)	156, 1011, 2850, 3750
AspLEI	GCGC	2 cut(s)	73, 3588
AsuC2I	CCSGG	3 cut(s)	80, 81, 184
AsuHPI	GGTGA	5 cut(s)	503, 1190, 2438, 2449, 3056
AvaI	CYCGRG	3 cut(s)	79, 309, 708
AvaII	GGWCC	8 cut(s)	884, 1127, 1154, 2066, 2265, 2333, 3404, 3420
BaeGI	GKGCMC	5 cut(s)	53, 867, 1147, 2326, 2782
BaeI	ACNNNNGTAYC	2 cut(s)	237, 270
BamHI	GGATCC	1 cut(s)	3002
BanI	GGYRCC	1 cut(s)	1034
BanII	GRGCYC	4 cut(s)	432, 1945, 2782, 3788
BarI	GAAGNNNNNNTAC	4 cut(s)	1530, 1562, 1662, 1694
BbsI	GAAGAC	1 cut(s)	3131
Bbv12I	GWGCWC	2 cut(s)	432, 1147
BceAI	ACGGC	4 cut(s)	972, 1401, 2321, 3095
BcnI	CCSGG	3 cut(s)	80, 81, 184
BcoDI	GTCTC	2 cut(s)	1736, 3666
BfaI	CTAG	6 cut(s)	645, 723, 1518, 2309, 2394, 2925
BfmI	CTRYAG	6 cut(s)	147, 329, 1071, 1737, 2270, 3120
BfoI	RGCGCY	1 cut(s)	3589
BglII	AGATCT	1 cut(s)	2770
BlpI	GCTNAGC	1 cut(s)	1311
BmcAI	AGTACT	2 cut(s)	761, 2095
Bme18I	GGWCC	8 cut(s)	884, 1127, 1154, 2066, 2265, 2333, 3404, 3420
BmeT110I	CYCGRG	3 cut(s)	79, 309, 708
BmrI	ACTGGG	2 cut(s)	1391, 3584
BmsI	GCATC	7 cut(s)	130, 476, 721, 1186, 1964, 2569, 3511
BmuI	ACTGGG	2 cut(s)	1391, 3584
BpiI	GAAGAC	1 cut(s)	3131
BplI	GAGNNNNNCTC	6 cut(s)	414, 446, 1607, 1639, 2892, 2924
BpmI	CTGGAG	2 cut(s)	2973, 3087
Bpu1102I	GCTNAGC	1 cut(s)	1311
BpuEI	CTTGAG	2 cut(s)	2054, 2894
BpuMI	CCSGG	3 cut(s)	80, 81, 184
BsaI	GGTCTC	1 cut(s)	3666
BsaJI	CCNNGG	8 cut(s)	79, 183, 707, 1210, 1937, 2737, 3411, 3709
BsaXI	ACNNNNNCTCC	4 cut(s)	3563, 3593, 3644, 3674
Bsc4I	CCNNNNNNNGG	8 cut(s)	152, 397, 893, 950, 2342, 2474, 3417, 3773
Bse1I	ACTGG	8 cut(s)	746, 1157, 1386, 3072, 3423, 3433, 3491, 3590
Bse3DI	GCAATG	3 cut(s)	291, 1374, 2394
BseDI	CCNNGG	8 cut(s)	79, 183, 707, 1210, 1937, 2737, 3411, 3709
BseLI	CCNNNNNNNGG	8 cut(s)	152, 397, 893, 950, 2342, 2474, 3417, 3773
BseMI	GCAATG	3 cut(s)	291, 1374, 2394
BseMII	CTCAG	8 cut(s)	40, 926, 927, 1302, 1428, 2951, 3287, 3529
BseNI	ACTGG	8 cut(s)	746, 1157, 1386, 3072, 3423, 3433, 3491, 3590
BseRI	GAGGAG	4 cut(s)	413, 2352, 3546, 3780
BseSI	GKGCMC	5 cut(s)	53, 867, 1147, 2326, 2782
BseYI	CCCAGC	3 cut(s)	796, 995, 1490
BsgI	GTGCAG	3 cut(s)	2231, 2894, 3755
Bsh1236I	CGCG	2 cut(s)	1908, 3666
BshFI	GGCC	6 cut(s)	538, 1251, 1449, 1495, 1658, 2780
BshNI	GGYRCC	1 cut(s)	1034
BsiHKAI	GWGCWC	2 cut(s)	432, 1147
BsiHKCI	CYCGRG	3 cut(s)	79, 309, 708
BsiSI	CCGG	4 cut(s)	80, 183, 2069, 2475
BslFI	GGGAC	3 cut(s)	1012, 2354, 3433
BslI	CCNNNNNNNGG	8 cut(s)	152, 397, 893, 950, 2342, 2474, 3417, 3773
BsmAI	GTCTC	2 cut(s)	1736, 3666
BsmBI	CGTCTC	1 cut(s)	1736
BsmFI	GGGAC	3 cut(s)	1012, 2354, 3433
BsmI	GAATGC	3 cut(s)	2887, 3103, 3603
BsnI	GGCC	6 cut(s)	538, 1251, 1449, 1495, 1658, 2780
Bso31I	GGTCTC	1 cut(s)	3666
BsoBI	CYCGRG	3 cut(s)	79, 309, 708
Bsp120I	GGGCCC	1 cut(s)	2778
Bsp1286I	GDGCHC	8 cut(s)	53, 432, 867, 1147, 1945, 2326, 2782, 3788
Bsp143I	GATC	9 cut(s)	75, 121, 1088, 1261, 1822, 2733, 2770, 3002, 3463
Bsp1720I	GCTNAGC	1 cut(s)	1311
BspACI	CCGC	5 cut(s)	319, 1086, 1628, 1908, 3666
BspANI	GGCC	6 cut(s)	538, 1251, 1449, 1495, 1658, 2780
BspCNI	CTCAG	8 cut(s)	39, 925, 928, 1303, 1429, 2950, 3286, 3528
BspFNI	CGCG	2 cut(s)	1908, 3666
BspHI	TCATGA	1 cut(s)	436
BspMAI	CTGCAG	2 cut(s)	333, 3124
BspPI	GGATC	7 cut(s)	70, 116, 1096, 2728, 2997, 3010, 3458
BspT107I	GGYRCC	1 cut(s)	1034
BspTNI	GGTCTC	1 cut(s)	3666
BsrDI	GCAATG	3 cut(s)	291, 1374, 2394
BsrI	ACTGG	8 cut(s)	746, 1157, 1386, 3072, 3423, 3433, 3491, 3590
BssECI	CCNNGG	8 cut(s)	79, 183, 707, 1210, 1937, 2737, 3411, 3709
BssMI	GATC	9 cut(s)	75, 121, 1088, 1261, 1822, 2733, 2770, 3002, 3463
BssNAI	GTATAC	1 cut(s)	603
Bst1107I	GTATAC	1 cut(s)	603
Bst6I	CTCTTC	4 cut(s)	1599, 2716, 2954, 2976
BstAPI	GCANNNNNTGC	1 cut(s)	1702
BstC8I	GCNNGC	3 cut(s)	1477, 2597, 2750
BstDSI	CCRYGG	1 cut(s)	1937
BstEII	GGTNACC	2 cut(s)	338, 2455
BstENI	CCTNNNNNAGG	2 cut(s)	150, 395
BstFNI	CGCG	2 cut(s)	1908, 3666
BstH2I	RGCGCY	1 cut(s)	3589
BstHHI	GCGC	2 cut(s)	73, 3588
BstKTI	GATC	9 cut(s)	78, 124, 1091, 1264, 1825, 2736, 2773, 3005, 3466
BstMAI	GTCTC	2 cut(s)	1736, 3666
BstMBI	GATC	9 cut(s)	75, 121, 1088, 1261, 1822, 2733, 2770, 3002, 3463
BstNSI	RCATGY	4 cut(s)	665, 1505, 2057, 2599
BstPI	GGTNACC	2 cut(s)	338, 2455
BstSFI	CTRYAG	6 cut(s)	147, 329, 1071, 1737, 2270, 3120
BstSLI	GKGCMC	5 cut(s)	53, 867, 1147, 2326, 2782
BstUI	CGCG	2 cut(s)	1908, 3666
BstV2I	GAAGAC	1 cut(s)	3131
BstX2I	RGATCY	4 cut(s)	75, 2733, 2770, 3002
BstYI	RGATCY	4 cut(s)	75, 2733, 2770, 3002
BstZ17I	GTATAC	1 cut(s)	603
BsuRI	GGCC	6 cut(s)	538, 1251, 1449, 1495, 1658, 2780
BtgI	CCRYGG	1 cut(s)	1937
BtsI	GCAGTG	2 cut(s)	2901, 3297
Cac8I	GCNNGC	3 cut(s)	1477, 2597, 2750
CciI	TCATGA	1 cut(s)	436
CfoI	GCGC	2 cut(s)	73, 3588
Cfr9I	CCCGGG	1 cut(s)	79
CsiI	ACCWGGT	1 cut(s)	2945
Csp6I	GTAC	8 cut(s)	246, 760, 1770, 1819, 1855, 2094, 2397, 3293
CviQI	GTAC	8 cut(s)	246, 760, 1770, 1819, 1855, 2094, 2397, 3293
DpnI	GATC	9 cut(s)	77, 123, 1090, 1263, 1824, 2735, 2772, 3004, 3465
DpnII	GATC	9 cut(s)	75, 121, 1088, 1261, 1822, 2733, 2770, 3002, 3463
DraI	TTTAAA	2 cut(s)	658, 1864
Eam1104I	CTCTTC	4 cut(s)	1599, 2716, 2954, 2976
EarI	CTCTTC	4 cut(s)	1599, 2716, 2954, 2976
EciI	GGCGGA	1 cut(s)	1101
Ecl136II	GAGCTC	1 cut(s)	430
Eco147I	AGGCCT	1 cut(s)	1658
Eco24I	GRGCYC	4 cut(s)	432, 1945, 2782, 3788
Eco31I	GGTCTC	1 cut(s)	3666
Eco32I	GATATC	2 cut(s)	3488, 3542
Eco47I	GGWCC	8 cut(s)	884, 1127, 1154, 2066, 2265, 2333, 3404, 3420
Eco53kI	GAGCTC	1 cut(s)	430
Eco57I	CTGAAG	4 cut(s)	660, 3038, 3099, 3737
Eco88I	CYCGRG	3 cut(s)	79, 309, 708
Eco91I	GGTNACC	2 cut(s)	338, 2455
EcoICRI	GAGCTC	1 cut(s)	430
EcoNI	CCTNNNNNAGG	2 cut(s)	150, 395
EcoO109I	RGGNCCY	1 cut(s)	2265
EcoO65I	GGTNACC	2 cut(s)	338, 2455
EcoRI	GAATTC	2 cut(s)	156, 165
EcoRV	GATATC	2 cut(s)	3488, 3542
EcoT22I	ATGCAT	1 cut(s)	736
EcoT38I	GRGCYC	4 cut(s)	432, 1945, 2782, 3788
Esp3I	CGTCTC	1 cut(s)	1736
FaqI	GGGAC	3 cut(s)	1012, 2354, 3433
FauI	CCCGC	1 cut(s)	1901
FauNDI	CATATG	1 cut(s)	3441
FblI	GTMKAC	1 cut(s)	602
FriOI	GRGCYC	4 cut(s)	432, 1945, 2782, 3788
FspBI	CTAG	6 cut(s)	645, 723, 1518, 2309, 2394, 2925
FspI	TGCGCA	1 cut(s)	72
GlaI	GCGC	2 cut(s)	72, 3587
GsaI	CCCAGC	3 cut(s)	800, 999, 1494
GsuI	CTGGAG	2 cut(s)	2973, 3087
HaeII	RGCGCY	1 cut(s)	3589
HaeIII	GGCC	6 cut(s)	538, 1251, 1449, 1495, 1658, 2780
HapII	CCGG	4 cut(s)	80, 183, 2069, 2475
HhaI	GCGC	2 cut(s)	73, 3588
Hin6I	GCGC	2 cut(s)	71, 3586
HinP1I	GCGC	2 cut(s)	71, 3586
HincII	GTYRAC	2 cut(s)	415, 2943
HindII	GTYRAC	2 cut(s)	415, 2943
HindIII	AAGCTT	3 cut(s)	2030, 2651, 3020
HpaII	CCGG	4 cut(s)	80, 183, 2069, 2475
HphI	GGTGA	5 cut(s)	503, 1190, 2438, 2449, 3056
Hpy166II	GTNNAC	7 cut(s)	415, 603, 1145, 1633, 2943, 3323, 3706
Hpy8I	GTNNAC	7 cut(s)	415, 603, 1145, 1633, 2943, 3323, 3706
Hpy99I	CGWCG	1 cut(s)	204
HpyCH4IV	ACGT	2 cut(s)	1745, 3261
HpySE526I	ACGT	2 cut(s)	1745, 3261
HspAI	GCGC	2 cut(s)	71, 3586
Kzo9I	GATC	9 cut(s)	75, 121, 1088, 1261, 1822, 2733, 2770, 3002, 3463
LweI	GCATC	7 cut(s)	130, 476, 721, 1186, 1964, 2569, 3511
MabI	ACCWGGT	1 cut(s)	2945
MaeI	CTAG	6 cut(s)	645, 723, 1518, 2309, 2394, 2925
MaeII	ACGT	2 cut(s)	1745, 3261
MalI	GATC	9 cut(s)	77, 123, 1090, 1263, 1824, 2735, 2772, 3004, 3465
MboI	GATC	9 cut(s)	75, 121, 1088, 1261, 1822, 2733, 2770, 3002, 3463
MfeI	CAATTG	2 cut(s)	1512, 3027
MflI	RGATCY	4 cut(s)	75, 2733, 2770, 3002
MhlI	GDGCHC	8 cut(s)	53, 432, 867, 1147, 1945, 2326, 2782, 3788
MlyI	GAGTC	3 cut(s)	301, 2171, 3438
MmeI	TCCRAC	2 cut(s)	537, 3517
Mph1103I	ATGCAT	1 cut(s)	736
MroXI	GAANNNNTTC	4 cut(s)	156, 1011, 2850, 3750
MslI	CAYNNNNRTG	6 cut(s)	441, 1364, 1506, 2424, 2469, 3549
MspA1I	CMGCKG	3 cut(s)	890, 2878, 3119
MspI	CCGG	4 cut(s)	80, 183, 2069, 2475
MunI	CAATTG	2 cut(s)	1512, 3027
Mva1269I	GAATGC	3 cut(s)	2887, 3103, 3603
MvnI	CGCG	2 cut(s)	1908, 3666
NciI	CCSGG	3 cut(s)	80, 81, 184
NdeI	CATATG	1 cut(s)	3441
NdeII	GATC	9 cut(s)	75, 121, 1088, 1261, 1822, 2733, 2770, 3002, 3463
NmuCI	GTSAC	4 cut(s)	304, 1918, 2455, 2968
NsbI	TGCGCA	1 cut(s)	72
NsiI	ATGCAT	1 cut(s)	736
NspI	RCATGY	4 cut(s)	665, 1505, 2057, 2599
PaeI	GCATGC	1 cut(s)	2599
PagI	TCATGA	1 cut(s)	436
PceI	AGGCCT	1 cut(s)	1658
PciI	ACATGT	1 cut(s)	661
PctI	GAATGC	3 cut(s)	2887, 3103, 3603
PdmI	GAANNNNTTC	4 cut(s)	156, 1011, 2850, 3750
PflMI	CCANNNNNTGG	1 cut(s)	893
PfoI	TCCNGGA	2 cut(s)	1650, 2587
PleI	GAGTC	3 cut(s)	301, 2170, 3437
PpsI	GAGTC	3 cut(s)	301, 2170, 3437
PpuMI	RGGWCCY	1 cut(s)	2265
PscI	ACATGT	1 cut(s)	661
Psp124BI	GAGCTC	1 cut(s)	432
Psp1406I	AACGTT	1 cut(s)	3261
Psp5II	RGGWCCY	1 cut(s)	2265
PspEI	GGTNACC	2 cut(s)	338, 2455
PspFI	CCCAGC	3 cut(s)	796, 995, 1490
PspOMI	GGGCCC	1 cut(s)	2778
PspPPI	RGGWCCY	1 cut(s)	2265
PstI	CTGCAG	2 cut(s)	333, 3124
PsuI	RGATCY	4 cut(s)	75, 2733, 2770, 3002
PvuII	CAGCTG	3 cut(s)	890, 2878, 3119
RsaI	GTAC	8 cut(s)	247, 761, 1771, 1820, 1856, 2095, 2398, 3294
RsaNI	GTAC	8 cut(s)	246, 760, 1770, 1819, 1855, 2094, 2397, 3293
RseI	CAYNNNNRTG	6 cut(s)	441, 1364, 1506, 2424, 2469, 3549
SacI	GAGCTC	1 cut(s)	432
Sau3AI	GATC	9 cut(s)	75, 121, 1088, 1261, 1822, 2733, 2770, 3002, 3463
ScaI	AGTACT	2 cut(s)	761, 2095
SchI	GAGTC	3 cut(s)	301, 2171, 3438
SduI	GDGCHC	8 cut(s)	53, 432, 867, 1147, 1945, 2326, 2782, 3788
SexAI	ACCWGGT	1 cut(s)	2945
SfaNI	GCATC	7 cut(s)	130, 476, 721, 1186, 1964, 2569, 3511
SfcI	CTRYAG	6 cut(s)	147, 329, 1071, 1737, 2270, 3120
SinI	GGWCC	8 cut(s)	884, 1127, 1154, 2066, 2265, 2333, 3404, 3420
SmaI	CCCGGG	1 cut(s)	81
SmiI	ATTTAAAT	1 cut(s)	1864
SmiMI	CAYNNNNRTG	6 cut(s)	441, 1364, 1506, 2424, 2469, 3549
SmlI	CTYRAG	2 cut(s)	2033, 2909
SmoI	CTYRAG	2 cut(s)	2033, 2909
SphI	GCATGC	1 cut(s)	2599
SseBI	AGGCCT	1 cut(s)	1658
SsiI	CCGC	5 cut(s)	319, 1086, 1628, 1908, 3666
SspI	AATATT	3 cut(s)	358, 1245, 3474
SspMI	CTAG	6 cut(s)	645, 723, 1518, 2309, 2394, 2925
SstI	GAGCTC	1 cut(s)	432
StuI	AGGCCT	1 cut(s)	1658
SwaI	ATTTAAAT	1 cut(s)	1864
TaiI	ACGT	2 cut(s)	1748, 3264
TaqI	TCGA	5 cut(s)	697, 786, 853, 1059, 1330
TatI	WGTACW	4 cut(s)	759, 2093, 2396, 3292
TseFI	GTSAC	4 cut(s)	304, 1918, 2455, 2968
Tsp45I	GTSAC	4 cut(s)	304, 1918, 2455, 2968
TspGWI	ACGGA	2 cut(s)	1821, 1872
TspMI	CCCGGG	1 cut(s)	79
Van91I	CCANNNNNTGG	1 cut(s)	893
VneI	GTGCAC	1 cut(s)	1143
VpaK11BI	GGWCC	8 cut(s)	884, 1127, 1154, 2066, 2265, 2333, 3404, 3420
XagI	CCTNNNNNAGG	2 cut(s)	150, 395
XceI	RCATGY	4 cut(s)	665, 1505, 2057, 2599
XmaI	CCCGGG	1 cut(s)	79
XmiI	GTMKAC	1 cut(s)	602
XmnI	GAANNNNTTC	4 cut(s)	156, 1011, 2850, 3750
XspI	CTAG	6 cut(s)	645, 723, 1518, 2309, 2394, 2925
ZrmI	AGTACT	2 cut(s)	761, 2095
Zsp2I	ATGCAT	1 cut(s)	736

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.