RchiOBHm_Chr1g0337071

Hydantoinase B/oxoprolinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0015430
Physical Location & Seq
Reverse (-)
28710405 .. 28716134
5730 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56499

Sequence Viewer

Length: 3792 bp
ATGGGGAGTGCCAATGCCAACAAGCTCAGATTTTGCATTGACAGAGGGGGCACATTCACCGATGTTTATGCTCAGATCCCGGGTCAGCCTGATGGTCGTGTTTTGAAACTTTTATCGGTTGATCCATCAAACTATGATGATGCTCCAGTGGAAGGAATTCGGAGAATTCTTGAAGAATTTACCGGGGAGAAAATTTCCCGTCGTTCAAAGTTACCAACTGATAAGATAGAGTGGATAAGGATGGGTACAACTGTTGCTACAAATGCACTTTTGGAGAGGAAAGGGGAAAGCATTGCTCTTTGTGTGACTCGGGGTTTCCGGAACTTGCTGCAGATTGGTAACCAGGCTCGTCCAAATATTTTTGACCTCACTGTATCTAAACCTTCAAACCTTTATGAGGAGGTTATTGAGGTTGACGAGAGAGTTGAGCTCATTCATGATAATGAAGATTCTGCTTCTGCATCATTAGTTAAAGGGGTTTCTGGTGAGATGGTTAAAGTTGTAAAGCCTCTTGATGTTAAAACATTAAGGCCATTACTCAAAGGTTTGTTGGAGAAGGGAATTAGCTGTTTGGCAGTTGTGTTAATGCACTCATATACTTACCCACAACATGAAATAGCTGTTGAAAGGTTAGCAGCTAGTATGGGCTTTAAACATGTTTCCTTATCTTCAGCTTTGACACCTATGGTTCGAGCTGTTCCTCGGGGTCTAACAGCTAGTGTTGATGCATATTTGACGCCAGTAATCAAAGAGTACTTATCAGGGTTCATGTCTCAGTTCGATGAAGGGCTGGGTAAGGTTAATGTTTTGTTTATGCAATCTGATGGAGGACTTGCACCAGAAAGTCGATTTTCAGGTCACAAAGCTGTTTTGTCAGGTCCAGCTGGTGGGGTTGTTGGTTACTCTCAGACACTTTTTGGGCTTGAAACTGAGAAACCTCTTATTGGGTTTGACATGGGTGGCACATCTACTGATGTGAGCCGTTATGCTGGGACTTATGAACAAGTTCTGGAAACTCAAATTGCTGGTGCCATCATACAAGCACCTCAACTCGACATAAATACTGTAGCTGCTGGTGGCGGATCAAAATTAAAGTTCCAATTTGGAGCTTTTCGGGTAGGACCAGAATCAGTGGGTGCACATCCAGGTCCAGTGTGTTACCGTAAAGGTGGTGAATTAGCAGTTACAGATGCAAACTTAGTCCTCGGATATGTTATTCCTGATTATTTCCCATCAATATTTGGCCCCAACGAAGATCAGCCTTTAGATATTGAGGAAACAAGAAAAGAATTTGAAAATCTTGCTGAGCAAATAAATTCCTATCGAAAGAGCCAGGATTCATCTGCAAAGGACATGACAGTGGAGGACATTGCACTGGGATTTGTGAATGTTGCCAATGAAACTATGTGCCGTCCGATAAGGCAATTAACTGAGATGAAGGGCCATGAAACAAAGAATCATGCACTTGCTTGCTTTGGAGGTGCTGGGCCTCAACATGCCTGTGCAATTGCTAGGTCATTGGGCATGAAAGAAGTATTCATACACAGGTTTTGTGGAATCTTAAGTGCTTATGGGATGGGTTTGGCAGATGTTGTTGAAGAGGCACAGGAGCCATACTCTGCGGTTTACAGTCTGGATTCTGTCCAGGAGGCCTCTCTCAGAGAAGCTGTTTTACTGAATCAAGTAAAGCAGAAGTTGCAAGAGCAAGGTTTTAGAGATGAAAACATGAAAACAGAGACATATTTGAATTTGAGATATGAAGGTACAGACACCTCAATCATGGTTAAGAAACGAAAAGACGGAGAATGTAAGTACGATCTGGACTTTGTGGAATTATTCCAGCAGGAGTACGGATTTAAATTACTGAATAGGAATATCCTAGTAAGTGATGTTAGGGTTCGCGGGGTAGGAGTGACTAATATATTGAAGCCACGGGCTCTGGAATCTGCATCTTGTTCTCCTAAAGTTGAAGGTAACTACAAGGTTTATTTTGAGAATGGGTGGCAAGAAACACCTCTATACAAGCTTGAGAATCTGGGTTATGGGCATGTCATACAGGGTCCGGCAATAATTATGAATGGGAATAGTACTGTGATAGTGGAACCTAAGTGCAAAGCTATGATAACCAAATATGGTAACATTAAAATTGAAATTGAGTCTGCTTCAAGCACCCTGAAAGTAGCAGAAAAGGTTGCAAATGTTGTGCAGCTTTCAATCTTTAATCATAGATTTATGGGTATAGCCGAACAGATGGGAAGGACCCTACAGAGAACTTCTATATCAACTAACATCAAGGAACGGCTAGATTTCTCATGTGCCCTCTTTGGACCTGACGGGGGATTAGTTGCTAATGCTCCTCATGTCCCTGTGCATTTAGGAGCAATGTCTAGTACAGTTCGGTGGCAGCTCAACTATTGGGGTGAAAATTTGAATGAAGGAGATGTTTTGGTCACCAATCATCCTTGTGCCGGAGGTAGCCATCTTCCTGATATAACTGTTATTACACCTGTATTTGACAATGGAAAGCTGGTATTTTTTGTGGCAAGTAGAGGGCATCATGCAGAGATAGGAGGTATTACTCCTGGAAGCATGCCACCATTTTCCAAGTCTATATGGGAAGAAGGGGCTGCTATAAAAGCATTTAAGCTTGTTGAAAAGGGGATTTTTCAAGAAGAAGGAATTACTAAACTTCTCTGCTTCCCCTCTTCTGATGACTTGGCACAAAAGATCCCAGGAACTCGCAGGCTTCAAGATAACTTATCAGATCTTCGGGCCCAAGTAGCTGCAAACCAGAGAGGAATTACTCTTATTAAAGAGCTTATTGAGCAGTATGGTTTGGAAATGGTTCAGGCTTACATGACCTATGTGCAGCTGAATGCAGAAGAAGCAGTGAGAGAAATGCTCAAGTCTGTCGCTGCTAGAGTTTTATCTCAGTCAACCAGGTCTGGAGAGAAGAGTTCTGTTACTATTGAAGAAGAGGATTACATGGATGATGGATCCATTATTCATCTGAAGCTTACAATTGATTCTGTTAAAGGTGAAGCAAATTTTGATTTTAGTGGAACCAGTCCTGAAGTATATGGGAATTGGAATGCTCCAGAAGCCGTAACAGCTGCAGCAGTTATATACTGTCTTCGCTGCTTGGTGGATGTTGATATACCTCTGAATCAAGGTTGTTTGGCTCCTGTGAAAATCTATATTCCACCTGGCTCATTCCTCTCTCCAAGTGATAAGGCTGCTGTGGTGGGTGGTAACGTTCTCACTTCTCAGAGAATAACTGATGTTGTACTCACTGCATTTCAGGCTTGTGCTTGTTCACAGGGTTGTATGAATAATCTCACTTTTGGGGATGACACATTTGGTTATTATGAAACAATTGGAGGTGGGAGTGGAGCTGGTCCATCCTGGGATGGGACCAGTGGAGTCCAGTGTCATATGACCAATACCCGAATGACTGATCCAGAAATATTTGAACAGAGATATCCAGTTCTTTTGCATCAGTTTTCCCTCAGAGCGAACAGTGGAGGAGTTGGATATCATAGAGGTGGTAATGGGCTTGTGAGGGAGATAGAGTTCAAGAGCCCAGTCGTTGTGAGCATTCTTTCAGAGAGGCGTGTTCATGCTCCGAGAGGGTTGAAAGGAGGGGAGGATGGGTCTCGCGGAGCTAACTACCTAATCACAAAAGATAAAAGAAGGGTTTACCTGGGTGGTAAGAACACAGTTGAGGTGCAGTCGGGGGAAATTCTTCAGATTTTAACTCCTGGTGGTGGTGGATGGGGCTCTCCTCTTTAA

Protein Analysis

1263

Amino Acids

137.6

Weight (kDa)

5.7

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydant_A_N PF05378 10 - 215 4.1e-57 Hydantoinase/oxoprolinase N-terminal region
Hydantoinase_A PF01968 235 - 534 2.1e-107 Hydantoinase/oxoprolinase
Hydant_A_C PF19278 558 - 711 3.3e-09 Hydantoinase/oxoprolinase C-terminal domain
Hydantoinase_B PF02538 735 - 1262 1.9e-214 Hydantoinase B/oxoprolinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37830
fragaria_vesca FvH4_7g07120 FvH4_7g07120
malus_domestica MD07G1077100.v1.1
prunus_persica Prupe.2G098100_v2.0.a1
pyrus_communis pycom07g06060
rosa_chinensis RchiOBHm_Chr1g0337071
rosa_laevigata RLG00000029307
rosa_multiflora Rmu_sc0010164.1_g000003
rosa_roxburghii Rroxscaffold_4G00314980
rosa_rugosa Rorug01G0130900
rosa_samantha Rh1AG151600 Rh1BG120200 Rh1CG142800 Rh1DG158800
rosa_wichuraiana Rw1G012400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1028
AccB7I CCANNNNNTGG 1 cut(s) 887
AccII CGCG 2 cut(s) 1902, 3660
AccIII TCCGGA 1 cut(s) 318
AciI CCGC 4 cut(s) 1080, 1622, 1902, 3660
AclI AACGTT 1 cut(s) 3255
AclWI GGATC 7 cut(s) 70, 116, 1090, 2722, 2991, 3004, 3452
AcuI CTGAAG 4 cut(s) 654, 3032, 3093, 3731
AcyI GRCGYC 1 cut(s) 737
AfaI GTAC 8 cut(s) 247, 755, 1765, 1814, 1850, 2089, 2392, 3288
AfiI CCNNNNNNNGG 8 cut(s) 152, 397, 887, 944, 2336, 2468, 3411, 3767
AflII CTTAAG 1 cut(s) 1561
AflIII ACRYGT 1 cut(s) 655
AloI GAACNNNNNNTCC 2 cut(s) 3557, 3589
Alw21I GWGCWC 2 cut(s) 432, 1141
Alw26I GTCTC 3 cut(s) 777, 1730, 3660
Alw44I GTGCAC 1 cut(s) 1137
AlwI GGATC 7 cut(s) 70, 116, 1090, 2722, 2991, 3004, 3452
Ama87I CYCGRG 3 cut(s) 79, 309, 702
Aor13HI TCCGGA 1 cut(s) 318
AoxI GGCC 6 cut(s) 530, 1243, 1441, 1487, 1650, 2772
ApaI GGGCCC 1 cut(s) 2776
ApaLI GTGCAC 1 cut(s) 1137
Asp700I GAANNNNTTC 4 cut(s) 156, 1005, 2844, 3744
AsuC2I CCSGG 3 cut(s) 80, 81, 184
AsuHPI GGTGA 6 cut(s) 49, 497, 1184, 2432, 2443, 3050
AvaI CYCGRG 3 cut(s) 79, 309, 702
AvaII GGWCC 8 cut(s) 878, 1121, 1148, 2060, 2259, 2327, 3398, 3414
BaeGI GKGCMC 4 cut(s) 53, 1141, 2320, 2776
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BamHI GGATCC 1 cut(s) 2996
BanI GGYRCC 1 cut(s) 1028
BanII GRGCYC 5 cut(s) 432, 1939, 2776, 3584, 3782
BarI GAAGNNNNNNTAC 4 cut(s) 1524, 1556, 1656, 1688
BbsI GAAGAC 1 cut(s) 3125
Bbv12I GWGCWC 2 cut(s) 432, 1141
BceAI ACGGC 4 cut(s) 966, 1395, 2315, 3089
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BcnI CCSGG 3 cut(s) 80, 81, 184
BcoDI GTCTC 3 cut(s) 777, 1730, 3660
BfaI CTAG 7 cut(s) 639, 717, 1512, 1880, 2303, 2388, 2919
BfmI CTRYAG 4 cut(s) 329, 1065, 2264, 3114
BfrI CTTAAG 1 cut(s) 1561
BglII AGATCT 1 cut(s) 2764
BlpI GCTNAGC 1 cut(s) 1305
BmcAI AGTACT 2 cut(s) 755, 2089
Bme18I GGWCC 8 cut(s) 878, 1121, 1148, 2060, 2259, 2327, 3398, 3414
BmeT110I CYCGRG 3 cut(s) 79, 309, 702
BmrI ACTGGG 2 cut(s) 1385, 3578
BmsI GCATC 7 cut(s) 130, 470, 715, 1180, 1958, 2563, 3505
BmuI ACTGGG 2 cut(s) 1385, 3578
BpiI GAAGAC 1 cut(s) 3125
BplI GAGNNNNNCTC 6 cut(s) 414, 446, 1601, 1633, 2886, 2918
BpmI CTGGAG 3 cut(s) 129, 2967, 3081
Bpu1102I GCTNAGC 1 cut(s) 1305
BpuEI CTTGAG 2 cut(s) 2048, 2888
BpuMI CCSGG 3 cut(s) 80, 81, 184
BsaHI GRCGYC 1 cut(s) 737
BsaI GGTCTC 1 cut(s) 3660
BsaJI CCNNGG 8 cut(s) 79, 183, 701, 1204, 1931, 2731, 3405, 3703
BsaWI WCCGGW 1 cut(s) 318
BsaXI ACNNNNNCTCC 4 cut(s) 3557, 3587, 3638, 3668
Bsc4I CCNNNNNNNGG 8 cut(s) 152, 397, 887, 944, 2336, 2468, 3411, 3767
Bse1I ACTGG 9 cut(s) 146, 740, 1151, 1380, 3066, 3417, 3427, 3485, 3584
Bse3DI GCAATG 3 cut(s) 291, 1368, 2388
BseAI TCCGGA 1 cut(s) 318
BseDI CCNNGG 8 cut(s) 79, 183, 701, 1204, 1931, 2731, 3405, 3703
BseLI CCNNNNNNNGG 8 cut(s) 152, 397, 887, 944, 2336, 2468, 3411, 3767
BseMI GCAATG 3 cut(s) 291, 1368, 2388
BseNI ACTGG 9 cut(s) 146, 740, 1151, 1380, 3066, 3417, 3427, 3485, 3584
BseRI GAGGAG 4 cut(s) 413, 2346, 3540, 3774
BseSI GKGCMC 4 cut(s) 53, 1141, 2320, 2776
BseYI CCCAGC 3 cut(s) 790, 989, 1484
BsgI GTGCAG 3 cut(s) 2225, 2888, 3749
Bsh1236I CGCG 2 cut(s) 1902, 3660
BshFI GGCC 6 cut(s) 532, 1245, 1443, 1489, 1652, 2774
BshNI GGYRCC 1 cut(s) 1028
BsiHKAI GWGCWC 2 cut(s) 432, 1141
BsiHKCI CYCGRG 3 cut(s) 79, 309, 702
BsiSI CCGG 5 cut(s) 80, 183, 319, 2063, 2469
BslFI GGGAC 3 cut(s) 1006, 2348, 3427
BslI CCNNNNNNNGG 8 cut(s) 152, 397, 887, 944, 2336, 2468, 3411, 3767
BsmAI GTCTC 3 cut(s) 777, 1730, 3660
BsmFI GGGAC 3 cut(s) 1006, 2348, 3427
BsmI GAATGC 3 cut(s) 2881, 3097, 3597
BsnI GGCC 6 cut(s) 532, 1245, 1443, 1489, 1652, 2774
Bso31I GGTCTC 1 cut(s) 3660
BsoBI CYCGRG 3 cut(s) 79, 309, 702
Bsp120I GGGCCC 1 cut(s) 2772
Bsp1286I GDGCHC 8 cut(s) 53, 432, 1141, 1939, 2320, 2776, 3584, 3782
Bsp13I TCCGGA 1 cut(s) 318
Bsp143I GATC 9 cut(s) 75, 121, 1082, 1255, 1816, 2727, 2764, 2996, 3457
Bsp1720I GCTNAGC 1 cut(s) 1305
BspACI CCGC 4 cut(s) 1080, 1622, 1902, 3660
BspANI GGCC 6 cut(s) 532, 1245, 1443, 1489, 1652, 2774
BspEI TCCGGA 1 cut(s) 318
BspFNI CGCG 2 cut(s) 1902, 3660
BspHI TCATGA 1 cut(s) 436
BspMAI CTGCAG 2 cut(s) 333, 3118
BspPI GGATC 7 cut(s) 70, 116, 1090, 2722, 2991, 3004, 3452
BspT107I GGYRCC 1 cut(s) 1028
BspTI CTTAAG 1 cut(s) 1561
BspTNI GGTCTC 1 cut(s) 3660
BsrDI GCAATG 3 cut(s) 291, 1368, 2388
BsrI ACTGG 9 cut(s) 146, 740, 1151, 1380, 3066, 3417, 3427, 3485, 3584
BssECI CCNNGG 8 cut(s) 79, 183, 701, 1204, 1931, 2731, 3405, 3703
BssMI GATC 9 cut(s) 75, 121, 1082, 1255, 1816, 2727, 2764, 2996, 3457
BssNI GRCGYC 1 cut(s) 737
Bst6I CTCTTC 4 cut(s) 1593, 2710, 2948, 2970
BstACI GRCGYC 1 cut(s) 737
BstAFI CTTAAG 1 cut(s) 1561
BstAPI GCANNNNNTGC 1 cut(s) 1696
BstC8I GCNNGC 3 cut(s) 1471, 2591, 2744
BstDSI CCRYGG 1 cut(s) 1931
BstEII GGTNACC 2 cut(s) 338, 2449
BstENI CCTNNNNNAGG 1 cut(s) 395
BstFNI CGCG 2 cut(s) 1902, 3660
BstKTI GATC 9 cut(s) 78, 124, 1085, 1258, 1819, 2730, 2767, 2999, 3460
BstMAI GTCTC 3 cut(s) 777, 1730, 3660
BstMBI GATC 9 cut(s) 75, 121, 1082, 1255, 1816, 2727, 2764, 2996, 3457
BstMWI GCNNNNNNNGC 9 cut(s) 263, 1696, 2636, 2780, 2824, 2885, 3101, 3110, 3302
BstNSI RCATGY 4 cut(s) 659, 1499, 2051, 2593
BstPI GGTNACC 2 cut(s) 338, 2449
BstSFI CTRYAG 4 cut(s) 329, 1065, 2264, 3114
BstSLI GKGCMC 4 cut(s) 53, 1141, 2320, 2776
BstUI CGCG 2 cut(s) 1902, 3660
BstV2I GAAGAC 1 cut(s) 3125
BstX2I RGATCY 4 cut(s) 75, 2727, 2764, 2996
BstYI RGATCY 4 cut(s) 75, 2727, 2764, 2996
BsuRI GGCC 6 cut(s) 532, 1245, 1443, 1489, 1652, 2774
BtgI CCRYGG 1 cut(s) 1931
BtsI GCAGTG 2 cut(s) 2895, 3291
Cac8I GCNNGC 3 cut(s) 1471, 2591, 2744
CciI TCATGA 1 cut(s) 436
Cfr9I CCCGGG 1 cut(s) 79
CseI GACGC 1 cut(s) 745
CsiI ACCWGGT 1 cut(s) 2939
Csp6I GTAC 8 cut(s) 246, 754, 1764, 1813, 1849, 2088, 2391, 3287
CviQI GTAC 8 cut(s) 246, 754, 1764, 1813, 1849, 2088, 2391, 3287
DpnI GATC 9 cut(s) 77, 123, 1084, 1257, 1818, 2729, 2766, 2998, 3459
DpnII GATC 9 cut(s) 75, 121, 1082, 1255, 1816, 2727, 2764, 2996, 3457
DraI TTTAAA 2 cut(s) 652, 1858
Eam1104I CTCTTC 4 cut(s) 1593, 2710, 2948, 2970
EarI CTCTTC 4 cut(s) 1593, 2710, 2948, 2970
EciI GGCGGA 1 cut(s) 1095
Ecl136II GAGCTC 1 cut(s) 430
Eco147I AGGCCT 1 cut(s) 1652
Eco24I GRGCYC 5 cut(s) 432, 1939, 2776, 3584, 3782
Eco31I GGTCTC 1 cut(s) 3660
Eco32I GATATC 2 cut(s) 3482, 3536
Eco47I GGWCC 8 cut(s) 878, 1121, 1148, 2060, 2259, 2327, 3398, 3414
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 4 cut(s) 654, 3032, 3093, 3731
Eco88I CYCGRG 3 cut(s) 79, 309, 702
Eco91I GGTNACC 2 cut(s) 338, 2449
EcoICRI GAGCTC 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 395
EcoO109I RGGNCCY 1 cut(s) 2259
EcoO65I GGTNACC 2 cut(s) 338, 2449
EcoRI GAATTC 2 cut(s) 156, 165
EcoRV GATATC 2 cut(s) 3482, 3536
EcoT22I ATGCAT 1 cut(s) 730
EcoT38I GRGCYC 5 cut(s) 432, 1939, 2776, 3584, 3782
FaqI GGGAC 3 cut(s) 1006, 2348, 3427
FauI CCCGC 1 cut(s) 1895
FauNDI CATATG 1 cut(s) 3435
FriOI GRGCYC 5 cut(s) 432, 1939, 2776, 3584, 3782
FspBI CTAG 7 cut(s) 639, 717, 1512, 1880, 2303, 2388, 2919
GsaI CCCAGC 3 cut(s) 794, 993, 1488
GsuI CTGGAG 3 cut(s) 129, 2967, 3081
HaeIII GGCC 6 cut(s) 532, 1245, 1443, 1489, 1652, 2774
HapII CCGG 5 cut(s) 80, 183, 319, 2063, 2469
HgaI GACGC 1 cut(s) 745
Hin1I GRCGYC 1 cut(s) 737
HincII GTYRAC 2 cut(s) 415, 2937
HindII GTYRAC 2 cut(s) 415, 2937
HindIII AAGCTT 3 cut(s) 2024, 2645, 3014
HpaII CCGG 5 cut(s) 80, 183, 319, 2063, 2469
HphI GGTGA 6 cut(s) 49, 497, 1184, 2432, 2443, 3050
Hpy166II GTNNAC 6 cut(s) 415, 1139, 1627, 2937, 3317, 3700
Hpy8I GTNNAC 6 cut(s) 415, 1139, 1627, 2937, 3317, 3700
Hpy99I CGWCG 1 cut(s) 204
HpyCH4IV ACGT 1 cut(s) 3255
HpyF10VI GCNNNNNNNGC 9 cut(s) 263, 1696, 2636, 2780, 2824, 2885, 3101, 3110, 3302
HpySE526I ACGT 1 cut(s) 3255
Hsp92I GRCGYC 1 cut(s) 737
Kpn2I TCCGGA 1 cut(s) 318
Kzo9I GATC 9 cut(s) 75, 121, 1082, 1255, 1816, 2727, 2764, 2996, 3457
LweI GCATC 7 cut(s) 130, 470, 715, 1180, 1958, 2563, 3505
MabI ACCWGGT 1 cut(s) 2939
MaeI CTAG 7 cut(s) 639, 717, 1512, 1880, 2303, 2388, 2919
MaeII ACGT 1 cut(s) 3255
MalI GATC 9 cut(s) 77, 123, 1084, 1257, 1818, 2729, 2766, 2998, 3459
MboI GATC 9 cut(s) 75, 121, 1082, 1255, 1816, 2727, 2764, 2996, 3457
MfeI CAATTG 3 cut(s) 1506, 3021, 3375
MflI RGATCY 4 cut(s) 75, 2727, 2764, 2996
MhlI GDGCHC 8 cut(s) 53, 432, 1141, 1939, 2320, 2776, 3584, 3782
MlyI GAGTC 3 cut(s) 301, 2165, 3432
MmeI TCCRAC 2 cut(s) 531, 3511
Mph1103I ATGCAT 1 cut(s) 730
MroI TCCGGA 1 cut(s) 318
MroXI GAANNNNTTC 4 cut(s) 156, 1005, 2844, 3744
MslI CAYNNNNRTG 5 cut(s) 441, 1358, 1500, 2463, 3543
MspA1I CMGCKG 3 cut(s) 884, 2872, 3113
MspCI CTTAAG 1 cut(s) 1561
MspI CCGG 5 cut(s) 80, 183, 319, 2063, 2469
MunI CAATTG 3 cut(s) 1506, 3021, 3375
Mva1269I GAATGC 3 cut(s) 2881, 3097, 3597
MvnI CGCG 2 cut(s) 1902, 3660
MwoI GCNNNNNNNGC 9 cut(s) 263, 1696, 2636, 2780, 2824, 2885, 3101, 3110, 3302
NciI CCSGG 3 cut(s) 80, 81, 184
NdeI CATATG 1 cut(s) 3435
NdeII GATC 9 cut(s) 75, 121, 1082, 1255, 1816, 2727, 2764, 2996, 3457
NmuCI GTSAC 4 cut(s) 304, 857, 1912, 2449
NsiI ATGCAT 1 cut(s) 730
NspI RCATGY 4 cut(s) 659, 1499, 2051, 2593
PaeI GCATGC 1 cut(s) 2593
PagI TCATGA 1 cut(s) 436
PceI AGGCCT 1 cut(s) 1652
PciI ACATGT 1 cut(s) 655
PctI GAATGC 3 cut(s) 2881, 3097, 3597
PdmI GAANNNNTTC 4 cut(s) 156, 1005, 2844, 3744
PflMI CCANNNNNTGG 1 cut(s) 887
PfoI TCCNGGA 2 cut(s) 1644, 2581
PleI GAGTC 3 cut(s) 301, 2164, 3431
PpsI GAGTC 3 cut(s) 301, 2164, 3431
PpuMI RGGWCCY 1 cut(s) 2259
PscI ACATGT 1 cut(s) 655
Psp124BI GAGCTC 1 cut(s) 432
Psp1406I AACGTT 1 cut(s) 3255
Psp5II RGGWCCY 1 cut(s) 2259
PspEI GGTNACC 2 cut(s) 338, 2449
PspFI CCCAGC 3 cut(s) 790, 989, 1484
PspOMI GGGCCC 1 cut(s) 2772
PspPPI RGGWCCY 1 cut(s) 2259
PstI CTGCAG 2 cut(s) 333, 3118
PsuI RGATCY 4 cut(s) 75, 2727, 2764, 2996
PvuII CAGCTG 3 cut(s) 884, 2872, 3113
RsaI GTAC 8 cut(s) 247, 755, 1765, 1814, 1850, 2089, 2392, 3288
RsaNI GTAC 8 cut(s) 246, 754, 1764, 1813, 1849, 2088, 2391, 3287
RseI CAYNNNNRTG 5 cut(s) 441, 1358, 1500, 2463, 3543
SacI GAGCTC 1 cut(s) 432
Sau3AI GATC 9 cut(s) 75, 121, 1082, 1255, 1816, 2727, 2764, 2996, 3457
ScaI AGTACT 2 cut(s) 755, 2089
SchI GAGTC 3 cut(s) 301, 2165, 3432
SduI GDGCHC 8 cut(s) 53, 432, 1141, 1939, 2320, 2776, 3584, 3782
SexAI ACCWGGT 1 cut(s) 2939
SfaNI GCATC 7 cut(s) 130, 470, 715, 1180, 1958, 2563, 3505
SfcI CTRYAG 4 cut(s) 329, 1065, 2264, 3114
SinI GGWCC 8 cut(s) 878, 1121, 1148, 2060, 2259, 2327, 3398, 3414
SmaI CCCGGG 1 cut(s) 81
SmiI ATTTAAAT 1 cut(s) 1858
SmiMI CAYNNNNRTG 5 cut(s) 441, 1358, 1500, 2463, 3543
SmlI CTYRAG 3 cut(s) 1561, 2027, 2903
SmoI CTYRAG 3 cut(s) 1561, 2027, 2903
SphI GCATGC 1 cut(s) 2593
SseBI AGGCCT 1 cut(s) 1652
SsiI CCGC 4 cut(s) 1080, 1622, 1902, 3660
SspI AATATT 3 cut(s) 358, 1239, 3468
SspMI CTAG 7 cut(s) 639, 717, 1512, 1880, 2303, 2388, 2919
SstI GAGCTC 1 cut(s) 432
StuI AGGCCT 1 cut(s) 1652
SwaI ATTTAAAT 1 cut(s) 1858
TaiI ACGT 1 cut(s) 3258
TaqI TCGA 5 cut(s) 691, 780, 847, 1053, 1324
TatI WGTACW 4 cut(s) 753, 2087, 2390, 3286
TseFI GTSAC 4 cut(s) 304, 857, 1912, 2449
Tsp45I GTSAC 4 cut(s) 304, 857, 1912, 2449
TspGWI ACGGA 2 cut(s) 1815, 1866
TspMI CCCGGG 1 cut(s) 79
Van91I CCANNNNNTGG 1 cut(s) 887
Vha464I CTTAAG 1 cut(s) 1561
VneI GTGCAC 1 cut(s) 1137
VpaK11BI GGWCC 8 cut(s) 878, 1121, 1148, 2060, 2259, 2327, 3398, 3414
XagI CCTNNNNNAGG 1 cut(s) 395
XceI RCATGY 4 cut(s) 659, 1499, 2051, 2593
XmaI CCCGGG 1 cut(s) 79
XmnI GAANNNNTTC 4 cut(s) 156, 1005, 2844, 3744
XspI CTAG 7 cut(s) 639, 717, 1512, 1880, 2303, 2388, 2919
ZrmI AGTACT 2 cut(s) 755, 2089
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.