pycom07g06060

Hydantoinase B/oxoprolinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015430
Physical Location & Seq
Reverse (-)
5353998 .. 5357804
3807 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g06060.1

Sequence Viewer

Length: 3807 bp
ATGGGGAGTGTCAATGACAACAAGCTCAGATTTTGCATTGACAGAGGGGGAACCTTCACTGATGTCTATGCTGAGATCCCAGGTCAGCCTGATGGACGGGTTTTAAAACTTTTGTCAGTAGATCCATCAAACTACGATGATGCTCCTGTCGAAGGAATTCGGAGGATTCTTGAAGAGTTTACGGGGAAGAAGATTTCCCGTGCTTCAAAAATTCCCACTGATAAGATAGAGTGGATAAGAATGGGTACAACTGTAGCAACTAATGCACTTTTGGAAAGAAAAGGGGAAAGGATTGCTCTTTGTGTGACTCGAGGTTTTCGTGATTTGCTTCAGATTGGCAACCAGGCTCGTCCCAAGATATTTGACCTCACTGTATCAAAACCATCAAACCTTTATGAGGAGGTTATTGAAGTTGATGAGAGAGTTGAGCTAGCTCGTGATAATGAAGATTCTTCTTCTTCACCATCATTAGTTAAAGGGATTTCTGGTGAGATGGTTAGAGTTGTAAAGCCTATTGATGTAGAAACTTTGAAACCGTTACTCCAAGGTTTGTTGAAGAAGGGCATTAGCTGTTTGGCTGTTGTGTTAATGCATTCATATACTTACCCACAACATGAAGTAGCGGTTGCAAGCGTAGCGGAGAATTTGGGCTTCAGACATGTGTCCTTATCTTCAGCGTTGACACCCATGGTTCGAGCTGTTCCTCGGGGTCTAACAGCTAGTGTTGATGCATATTTGACGCCAGTAATTAAAGAGTATCTAACAGGGTTTATGTCAAAATTTGATGAAGGAGTGGAGAAGGTGAATGTTTTGTTTATGCAATCTGATGGTGGACTTGCACCAGAAAGTCGATTTTCAGGGCATAAAGCTGTTTTATCTGGTCCTGCTGGTGGGGTTGTGGGTTACTCACAAACACTTTTTGGGCTTGAAACTGAGAAACCTCTCATTGGGTTTGACATGGGTGGCACATCTACAGATGTGAGCCGTTATGCTGGGACTTATGAACAAGTCCTGGAAACCCAGATTGCAGGTACCATAATACAAGCACCTCAGCTTGACATAAGTACAGTAGCTGCTGGTGGTGGATCAAAGTTGAAGTTCCAGTTTGGATCTTTCCGGGTAGGACCGGAATCAGTGGGTGCACATCCTGGTCCAGTATGTTACCGGAAAGGTGGGGAGTTGGCAGTTACAGATGCAAACTTAGTTCTCGGATATGTAATTCCTGATTATTTCCCACCAATATTTGGGCCCAATGAAGATGAGCCTCTAGATATCAAGGCAACAAGAGACAAGTTTGCGAACCTTTCAAGCCAAATAAATTCCTACCGGAAAAGCCAGGATCCATCTGCAAAGGACATGACAGTGGAGGACATTGCACTGGGATTTGTAAATGTTGCCAATGAGACTATGTGCCGTCCGATAAGGCAATTAACGGAGATGAAGGGCCATGAAACAAGGAACCATGCCCTTGCTTGCTTTGGAGGTGCTGGGCCTCAGCATGCCTGTGCAATTGCTAGATCATTGGGTATGAAAGAAGTACTTGTACACAGATTTTGTGGAATCTTAAGTGCTTATGGGATGGGATTGGCAGATGTTGTAGAAGACGCACAGGAGCCGTACTCTGCAGTTTACAGTCTTAAATCTGTCGAGGAAGCCTCTCACAGGGAAGCTACTTTATTGAATCAAGTAAAGAAGAAGCTGCACGATCAAGGTTTTAAAGATGAAAACATGACAACAGAGACATATCTGAATTTGAGATATGAAGGTACGGACACCTCAATCATGGTTAAGAAACGAATTTCTGAAGATGGGAGAGGATGTAACTATGATATCGACTTTGTGGAACTATTCCAGCAGGAGTACGGTTTTAAATTACTGAATAGGAATATCCTAATATGTGATGTTAGGGTTCGTGGGGAAGGAGTCACTAATATATTGAAGCCCCTGCCTCTGGAATCTACCTCATGTTCTCCTAAAATTGAAGGTAATTACAAGGTTTATTTTGGGAGTGGGTGGCTGGAAACACCTCTTTACAAGCTTGAGAAACTCGGGTATGGGCATATCATACCTGGCCCGGCAATAATCATGAATGGGAATAGTACTGTGATAGTAGAACCTAACTGTAAAGCTATAATAACCAAATATGGTAACATTAGAATTGAAATTGACTCTACTCCAAGCACCGTGAAAGTAGCGGACAAAGTTGCTAATGTTGTGCAGCTCTCAATCTTCAATCATAGATTTATGGGTATAGCCGAACAGATGGGAAGGACCCTGCAGAGAACTTCCATATCAACCAATATCAAGGAGCGGCTAGATTTCTCTTGTGCCCTCTTTGGACCTGATGGGGGACTAGTTGCCAATGCCCCTCATGTCCCTGTGCATTTAGGAGCAATGTCCAGTACTGTCCGGTGGCAGATCAATTACTGGGGCGACAACTTGAATGAAGGAGATGTTTTGGTCACCAATCATCCTTGTGCCGGAGGTAGTCATCTTCCTGATATAACTGTTGTTACCCCTGTATTTGATAAAGGAAGGCTGGTGTTTTTTGTGGCGAGTAGAGGGCATCATGCAGAGATTGGGGGTATTACTCCTGGAAGCATGCCACCTTTTTCCAAATCTATATGGGAAGAAGGGGCTGCCATAAAAGCGTTCAAGCTTGTGGAAAAGGGAATTTTTCAAGAAGACGGAATCATTCAACTTCTTCGATTCCCCTGTTCTGATGAATTGGTTCAAAAGATCCCAGGCACTCGCAGGATTCAAGATAATCTGTCAGATCTTCGAGCTCAAGTAGCTGCGAACCAGAGGGGAATTACGCTTATCAAAGAGCTTATTGAGCAGTATGGTTTGGACACTGTTCAGGCTTACATGACCTATGTACAGCTAAATGCAGAAGAAGCAGTGAGAGAAATGCTTAAGTCTGTTGCTGCTAGAGTTTTATCTCAGTCGGCTAGTTCTGGAGATCAGAGTTCTGTTACTATTGAAGAAGAGGATTACATGGATGATGGATCCGTTATTCATTTAAAACTTACAATTGATTCTGTTAAAGGGGAAGCAAATTTTGATTTCAGTGGAACCAGTCCGGAAGTATATGGGAATTGGAATGCTCCAGAAGCCGTAACTGCTGCAGCAGTTATATACTGTCTTCGCTGCTTGGTGGATGTTGACATTCCTCTAAATCAAGGTTGTTTGGCTCCTGTGAAAATCTATATTCCACCAGGCTCTTTCCTCTCTCCAAGTGATAAGGCTGCTGTCGTGGGTGGTAACGTTCTCACTTCTCAGAGAATAACTGACGTTGTGCTTACTGCATTCCAAGCTTGTGCTTGTTCACAGGGTTGTATGAATAATCTCACTTTTGGGGATAATACATTTGGTTATTATGAAACAATCGGAGGTGGGAGTGGAGCTGGTCCAACTTGGGATGGGACGAGTGGAGTGCAGTGTCATATGACCAATACCCGAATATGGACTGATCCAGAAATTTTCGAACAGAGATATCCTGTTCTTTTGCATAAATTTGGCCTGAGAGAGAACAGCGGCGGAGTTGGGTATCATAGAGGGGGTGATGGGCTTGTAAGGGAGATAGAGTTCAAGCGCCCAATTGTTGTGAGCATTCTTTCAGAGAGGCGTGTTCACGCACCAAGGGGGTTGAAAGGAGGGAAAGATGGGGCTCGGGGGGCTAACTTCCTAATCACAAAAGATAAGCGAAGGGTTTATCTTGGCGGGAAGAACACTGTTGATTTGCAATCAGGGGAAATTCTTCAGATTTTAACTCCTGGTGGTGGTGGATGGGGCTCTCCTCGTTGA

Protein Analysis

1269

Amino Acids

138.12

Weight (kDa)

5.93

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydant_A_N PF05378 10 - 216 1.6e-58 Hydantoinase/oxoprolinase N-terminal region
Hydantoinase_A PF01968 236 - 535 3.5e-105 Hydantoinase/oxoprolinase
Hydant_A_C PF19278 558 - 714 8.5e-08 Hydantoinase/oxoprolinase C-terminal domain
Hydantoinase_B PF02538 739 - 1267 2.4e-212 Hydantoinase B/oxoprolinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37830
fragaria_vesca FvH4_7g07120 FvH4_7g07120
malus_domestica MD07G1077100.v1.1
prunus_persica Prupe.2G098100_v2.0.a1
pyrus_communis pycom07g06060
rosa_chinensis RchiOBHm_Chr1g0337071
rosa_laevigata RLG00000029307
rosa_multiflora Rmu_sc0010164.1_g000003
rosa_roxburghii Rroxscaffold_4G00314980
rosa_rugosa Rorug01G0130900
rosa_samantha Rh1AG151600 Rh1BG120200 Rh1CG142800 Rh1DG158800
rosa_wichuraiana Rw1G012400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1019
Acc65I GGTACC 1 cut(s) 1031
AccB1I GGYRCC 1 cut(s) 1031
AccB7I CCANNNNNTGG 1 cut(s) 1244
AccBSI CCGCTC 1 cut(s) 2311
AccIII TCCGGA 1 cut(s) 3082
AciI CCGC 7 cut(s) 623, 638, 2195, 2311, 3537, 3540, 3723
AclI AACGTT 1 cut(s) 3267
AcuI CTGAAG 5 cut(s) 314, 637, 657, 1824, 3746
AcyI GRCGYC 1 cut(s) 740
AflII CTTAAG 2 cut(s) 1564, 2915
AflIII ACRYGT 1 cut(s) 658
AhlI ACTAGT 1 cut(s) 2353
AloI GAACNNNNNNTCC 2 cut(s) 3572, 3604
Alw21I GWGCWC 2 cut(s) 1144, 2788
Alw26I GTCTC 3 cut(s) 1281, 1397, 1733
Alw44I GTGCAC 1 cut(s) 1140
AlwNI CAGNNNCTG 1 cut(s) 1073
Ama87I CYCGRG 4 cut(s) 309, 705, 2048, 3672
Aor13HI TCCGGA 1 cut(s) 3082
AoxI GGCC 5 cut(s) 1247, 1444, 1490, 2071, 3520
ApaI GGGCCC 1 cut(s) 1251
ApaLI GTGCAC 1 cut(s) 1140
Asp700I GAANNNNTTC 3 cut(s) 156, 2730, 3759
Asp718I GGTACC 1 cut(s) 1031
AspLEI GCGC 1 cut(s) 3597
AsuC2I CCSGG 2 cut(s) 1118, 2075
AsuHPI GGTGA 5 cut(s) 453, 500, 814, 2455, 3575
AsuII TTCGAA 1 cut(s) 3486
AsuNHI GCTAGC 1 cut(s) 430
AvaI CYCGRG 4 cut(s) 309, 705, 2048, 3672
AvaII GGWCC 6 cut(s) 881, 1124, 1151, 2271, 2339, 3410
BaeGI GKGCMC 3 cut(s) 1144, 1251, 2332
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BamHI GGATCC 2 cut(s) 1339, 3008
BanI GGYRCC 1 cut(s) 1031
BanII GRGCYC 4 cut(s) 1251, 2788, 3673, 3797
BarI GAAGNNNNNNTAC 2 cut(s) 1527, 1559
BauI CACGAG 1 cut(s) 435
BbsI GAAGAC 3 cut(s) 1608, 2691, 3137
Bbv12I GWGCWC 2 cut(s) 1144, 2788
BbvCI CCTCAGC 2 cut(s) 1050, 1494
BceAI ACGGC 4 cut(s) 969, 1398, 1600, 3101
BcgI CGANNNNNNTGC 2 cut(s) 3418, 3452
BcnI CCSGG 2 cut(s) 1118, 2075
BcoDI GTCTC 3 cut(s) 1281, 1397, 1733
BcuI ACTAGT 1 cut(s) 2353
BfaI CTAG 8 cut(s) 431, 720, 1268, 1515, 2315, 2354, 2931, 2952
BfmI CTRYAG 5 cut(s) 252, 972, 1623, 2276, 3126
BfoI RGCGCY 1 cut(s) 3598
BfrI CTTAAG 2 cut(s) 1564, 2915
BfuAI ACCTGC 1 cut(s) 1019
BglII AGATCT 1 cut(s) 2776
BmcAI AGTACT 3 cut(s) 1539, 2101, 2404
Bme18I GGWCC 6 cut(s) 881, 1124, 1151, 2271, 2339, 3410
BmeT110I CYCGRG 4 cut(s) 309, 705, 2048, 3672
BmrI ACTGGG 2 cut(s) 1388, 2437
BmsI GCATC 4 cut(s) 130, 718, 1183, 2575
BmtI GCTAGC 1 cut(s) 434
BmuI ACTGGG 2 cut(s) 1388, 2437
BoxI GACNNNNGTC 1 cut(s) 661
BpiI GAAGAC 3 cut(s) 1608, 2691, 3137
BplI GAGNNNNNCTC 4 cut(s) 1604, 1636, 1640, 1672
BpmI CTGGAG 2 cut(s) 2979, 3093
Bpu10I CCTNAGC 2 cut(s) 1050, 1494
Bpu14I TTCGAA 1 cut(s) 3486
BpuEI CTTGAG 2 cut(s) 2060, 2772
BpuMI CCSGG 2 cut(s) 1118, 2075
BsaHI GRCGYC 1 cut(s) 740
BsaJI CCNNGG 6 cut(s) 79, 544, 687, 704, 2743, 3641
BsaWI WCCGGW 5 cut(s) 1126, 1164, 1326, 2409, 3082
BsaXI ACNNNNNCTCC 6 cut(s) 525, 555, 1804, 1834, 3572, 3602
Bse1I ACTGG 7 cut(s) 743, 1102, 1154, 1383, 2400, 2432, 3078
Bse3DI GCAATG 2 cut(s) 1371, 2400
BseAI TCCGGA 1 cut(s) 3082
BseDI CCNNGG 6 cut(s) 79, 544, 687, 704, 2743, 3641
BseGI GGATG 8 cut(s) 1144, 1584, 1823, 2470, 3007, 3166, 3427, 3794
BseMI GCAATG 2 cut(s) 1371, 2400
BseMII CTCAG 8 cut(s) 40, 63, 924, 1064, 1508, 2957, 3293, 3515
BseNI ACTGG 7 cut(s) 743, 1102, 1154, 1383, 2400, 2432, 3078
BseRI GAGGAG 2 cut(s) 413, 3789
BseSI GKGCMC 3 cut(s) 1144, 1251, 2332
BseYI CCCAGC 2 cut(s) 992, 1487
BsgI GTGCAG 3 cut(s) 1685, 2237, 3458
BshFI GGCC 5 cut(s) 1249, 1446, 1492, 2073, 3522
BshNI GGYRCC 1 cut(s) 1031
BsiHKAI GWGCWC 2 cut(s) 1144, 2788
BsiHKCI CYCGRG 4 cut(s) 309, 705, 2048, 3672
BsiSI CCGG 8 cut(s) 1117, 1127, 1165, 1327, 2075, 2410, 2481, 3083
BslFI GGGAC 5 cut(s) 336, 1009, 2360, 2364, 3439
BsmAI GTCTC 3 cut(s) 1281, 1397, 1733
BsmFI GGGAC 5 cut(s) 336, 1009, 2360, 2364, 3439
BsmI GAATGC 4 cut(s) 592, 3109, 3308, 3612
BsnI GGCC 5 cut(s) 1249, 1446, 1492, 2073, 3522
BsoBI CYCGRG 4 cut(s) 309, 705, 2048, 3672
Bsp119I TTCGAA 1 cut(s) 3486
Bsp120I GGGCCC 1 cut(s) 1247
Bsp1286I GDGCHC 6 cut(s) 1144, 1251, 2332, 2788, 3673, 3797
Bsp13I TCCGGA 1 cut(s) 3082
Bsp1407I TGTACA 2 cut(s) 1543, 2878
Bsp19I CCATGG 1 cut(s) 687
BspACI CCGC 7 cut(s) 623, 638, 2195, 2311, 3537, 3540, 3723
BspANI GGCC 5 cut(s) 1249, 1446, 1492, 2073, 3522
BspCNI CTCAG 8 cut(s) 39, 64, 925, 1063, 1507, 2956, 3292, 3516
BspEI TCCGGA 1 cut(s) 3082
BspHI TCATGA 1 cut(s) 2085
BspMAI CTGCAG 3 cut(s) 1627, 2280, 3130
BspMI ACCTGC 1 cut(s) 1019
BspOI GCTAGC 1 cut(s) 434
BspT104I TTCGAA 1 cut(s) 3486
BspT107I GGYRCC 1 cut(s) 1031
BspTI CTTAAG 2 cut(s) 1564, 2915
BsrBI CCGCTC 1 cut(s) 2311
BsrDI GCAATG 2 cut(s) 1371, 2400
BsrGI TGTACA 2 cut(s) 1543, 2878
BsrI ACTGG 7 cut(s) 743, 1102, 1154, 1383, 2400, 2432, 3078
BssECI CCNNGG 6 cut(s) 79, 544, 687, 704, 2743, 3641
BssNI GRCGYC 1 cut(s) 740
BssSI CACGAG 1 cut(s) 435
BssT1I CCWWGG 3 cut(s) 544, 687, 3641
Bst2BI CACGAG 1 cut(s) 435
Bst6I CTCTTC 2 cut(s) 168, 2982
BstACI GRCGYC 1 cut(s) 740
BstAFI CTTAAG 2 cut(s) 1564, 2915
BstAPI GCANNNNNTGC 1 cut(s) 263
BstAUI TGTACA 2 cut(s) 1543, 2878
BstBI TTCGAA 1 cut(s) 3486
BstC8I GCNNGC 5 cut(s) 432, 631, 1474, 1500, 2603
BstDEI CTNAG 9 cut(s) 26, 72, 933, 1050, 1201, 1494, 2943, 3279, 3524
BstDSI CCRYGG 1 cut(s) 687
BstEII GGTNACC 1 cut(s) 2461
BstENI CCTNNNNNAGG 3 cut(s) 150, 395, 1660
BstF5I GGATG 8 cut(s) 1144, 1584, 1823, 2470, 3007, 3166, 3427, 3794
BstH2I RGCGCY 1 cut(s) 3598
BstHHI GCGC 1 cut(s) 3597
BstMAI GTCTC 3 cut(s) 1281, 1397, 1733
BstNSI RCATGY 3 cut(s) 662, 1502, 2605
BstPAI GACNNNNGTC 1 cut(s) 661
BstPI GGTNACC 1 cut(s) 2461
BstSFI CTRYAG 5 cut(s) 252, 972, 1623, 2276, 3126
BstSLI GKGCMC 3 cut(s) 1144, 1251, 2332
BstV2I GAAGAC 3 cut(s) 1608, 2691, 3137
BstX2I RGATCY 7 cut(s) 75, 121, 1109, 1339, 2739, 2776, 3008
BstYI RGATCY 7 cut(s) 75, 121, 1109, 1339, 2739, 2776, 3008
BsuRI GGCC 5 cut(s) 1249, 1446, 1492, 2073, 3522
BtgI CCRYGG 1 cut(s) 687
BtsCI GGATG 8 cut(s) 1144, 1584, 1823, 2470, 3007, 3166, 3427, 3794
BtsI GCAGTG 2 cut(s) 2907, 3446
BveI ACCTGC 1 cut(s) 1019
Cac8I GCNNGC 5 cut(s) 432, 631, 1474, 1500, 2603
CaiI CAGNNNCTG 1 cut(s) 1073
CciI TCATGA 1 cut(s) 2085
CfoI GCGC 1 cut(s) 3597
CseI GACGC 2 cut(s) 748, 1613
DdeI CTNAG 9 cut(s) 26, 72, 933, 1050, 1201, 1494, 2943, 3279, 3524
DraI TTTAAA 4 cut(s) 105, 1717, 1870, 3024
Eam1104I CTCTTC 2 cut(s) 168, 2982
EarI CTCTTC 2 cut(s) 168, 2982
EciI GGCGGA 1 cut(s) 3555
Ecl136II GAGCTC 1 cut(s) 2786
Eco130I CCWWGG 3 cut(s) 544, 687, 3641
Eco24I GRGCYC 4 cut(s) 1251, 2788, 3673, 3797
Eco32I GATATC 3 cut(s) 1273, 1831, 3497
Eco47I GGWCC 6 cut(s) 881, 1124, 1151, 2271, 2339, 3410
Eco53kI GAGCTC 1 cut(s) 2786
Eco57I CTGAAG 5 cut(s) 314, 637, 657, 1824, 3746
Eco88I CYCGRG 4 cut(s) 309, 705, 2048, 3672
Eco91I GGTNACC 1 cut(s) 2461
EcoICRI GAGCTC 1 cut(s) 2786
EcoNI CCTNNNNNAGG 3 cut(s) 150, 395, 1660
EcoO109I RGGNCCY 1 cut(s) 2271
EcoO65I GGTNACC 1 cut(s) 2461
EcoRI GAATTC 1 cut(s) 156
EcoRV GATATC 3 cut(s) 1273, 1831, 3497
EcoT14I CCWWGG 3 cut(s) 544, 687, 3641
EcoT22I ATGCAT 2 cut(s) 594, 733
EcoT38I GRGCYC 4 cut(s) 1251, 2788, 3673, 3797
ErhI CCWWGG 3 cut(s) 544, 687, 3641
FalI AAGNNNNNCTT 2 cut(s) 1524, 1556
FaqI GGGAC 5 cut(s) 336, 1009, 2360, 2364, 3439
FauI CCCGC 1 cut(s) 3716
FauNDI CATATG 1 cut(s) 3447
FokI GGATG 8 cut(s) 1131, 1591, 1830, 2457, 3014, 3173, 3434, 3801
FriOI GRGCYC 4 cut(s) 1251, 2788, 3673, 3797
FspBI CTAG 8 cut(s) 431, 720, 1268, 1515, 2315, 2354, 2931, 2952
GlaI GCGC 1 cut(s) 3596
GsaI CCCAGC 2 cut(s) 996, 1491
GsuI CTGGAG 2 cut(s) 2979, 3093
HaeII RGCGCY 1 cut(s) 3598
HaeIII GGCC 5 cut(s) 1249, 1446, 1492, 2073, 3522
HapII CCGG 8 cut(s) 1117, 1127, 1165, 1327, 2075, 2410, 2481, 3083
HgaI GACGC 2 cut(s) 748, 1613
HhaI GCGC 1 cut(s) 3597
Hin1I GRCGYC 1 cut(s) 740
Hin6I GCGC 1 cut(s) 3595
HinP1I GCGC 1 cut(s) 3595
HincII GTYRAC 2 cut(s) 681, 3166
HindII GTYRAC 2 cut(s) 681, 3166
HindIII AAGCTT 3 cut(s) 2036, 2657, 3315
HpaII CCGG 8 cut(s) 1117, 1127, 1165, 1327, 2075, 2410, 2481, 3083
HphI GGTGA 5 cut(s) 453, 500, 814, 2455, 3575
Hpy166II GTNNAC 9 cut(s) 180, 681, 833, 1142, 1546, 1630, 3166, 3329, 3634
Hpy8I GTNNAC 9 cut(s) 180, 681, 833, 1142, 1546, 1630, 3166, 3329, 3634
HpyCH4IV ACGT 2 cut(s) 3267, 3294
HpyF3I CTNAG 9 cut(s) 26, 72, 933, 1050, 1201, 1494, 2943, 3279, 3524
HpySE526I ACGT 2 cut(s) 3267, 3294
Hsp92I GRCGYC 1 cut(s) 740
HspAI GCGC 1 cut(s) 3595
Kpn2I TCCGGA 1 cut(s) 3082
KpnI GGTACC 1 cut(s) 1035
LmnI GCTCC 7 cut(s) 148, 1612, 2308, 2390, 3112, 3199, 3404
LweI GCATC 4 cut(s) 130, 718, 1183, 2575
MaeI CTAG 8 cut(s) 431, 720, 1268, 1515, 2315, 2354, 2931, 2952
MaeII ACGT 2 cut(s) 3267, 3294
MbiI CCGCTC 1 cut(s) 2311
MfeI CAATTG 3 cut(s) 1509, 3033, 3600
MflI RGATCY 7 cut(s) 75, 121, 1109, 1339, 2739, 2776, 3008
MhlI GDGCHC 6 cut(s) 1144, 1251, 2332, 2788, 3673, 3797
MlyI GAGTC 3 cut(s) 301, 1932, 2162
MmeI TCCRAC 1 cut(s) 3437
Mph1103I ATGCAT 2 cut(s) 594, 733
MroI TCCGGA 1 cut(s) 3082
MroXI GAANNNNTTC 3 cut(s) 156, 2730, 3759
MslI CAYNNNNRTG 3 cut(s) 1361, 1503, 2475
MspA1I CMGCKG 1 cut(s) 3537
MspCI CTTAAG 2 cut(s) 1564, 2915
MspI CCGG 8 cut(s) 1117, 1127, 1165, 1327, 2075, 2410, 2481, 3083
MunI CAATTG 3 cut(s) 1509, 3033, 3600
Mva1269I GAATGC 4 cut(s) 592, 3109, 3308, 3612
NciI CCSGG 2 cut(s) 1118, 2075
NcoI CCATGG 1 cut(s) 687
NdeI CATATG 1 cut(s) 3447
NheI GCTAGC 1 cut(s) 430
NmuCI GTSAC 3 cut(s) 304, 1924, 2461
NsiI ATGCAT 2 cut(s) 594, 733
NspI RCATGY 3 cut(s) 662, 1502, 2605
NspV TTCGAA 1 cut(s) 3486
PaeI GCATGC 2 cut(s) 1502, 2605
PaeR7I CTCGAG 1 cut(s) 309
PagI TCATGA 1 cut(s) 2085
PciI ACATGT 1 cut(s) 658
PcsI WCGNNNNNNNCGW 1 cut(s) 316
PctI GAATGC 4 cut(s) 592, 3109, 3308, 3612
PdmI GAANNNNTTC 3 cut(s) 156, 2730, 3759
PflMI CCANNNNNTGG 1 cut(s) 1244
PfoI TCCNGGA 2 cut(s) 1011, 2593
PleI GAGTC 3 cut(s) 301, 1931, 2162
PpsI GAGTC 3 cut(s) 301, 1931, 2162
PpuMI RGGWCCY 1 cut(s) 2271
PscI ACATGT 1 cut(s) 658
PshAI GACNNNNGTC 1 cut(s) 661
Psp124BI GAGCTC 1 cut(s) 2788
Psp1406I AACGTT 1 cut(s) 3267
Psp5II RGGWCCY 1 cut(s) 2271
PspEI GGTNACC 1 cut(s) 2461
PspFI CCCAGC 2 cut(s) 992, 1487
PspOMI GGGCCC 1 cut(s) 1247
PspPPI RGGWCCY 1 cut(s) 2271
PspXI VCTCGAGB 1 cut(s) 309
PstI CTGCAG 3 cut(s) 1627, 2280, 3130
PstNI CAGNNNCTG 1 cut(s) 1073
PsuI RGATCY 7 cut(s) 75, 121, 1109, 1339, 2739, 2776, 3008
RseI CAYNNNNRTG 3 cut(s) 1361, 1503, 2475
SacI GAGCTC 1 cut(s) 2788
ScaI AGTACT 3 cut(s) 1539, 2101, 2404
SchI GAGTC 3 cut(s) 301, 1932, 2162
SduI GDGCHC 6 cut(s) 1144, 1251, 2332, 2788, 3673, 3797
SfaNI GCATC 4 cut(s) 130, 718, 1183, 2575
SfcI CTRYAG 5 cut(s) 252, 972, 1623, 2276, 3126
Sfr274I CTCGAG 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 3486
SinI GGWCC 6 cut(s) 881, 1124, 1151, 2271, 2339, 3410
SlaI CTCGAG 1 cut(s) 309
SmiMI CAYNNNNRTG 3 cut(s) 1361, 1503, 2475
SmlI CTYRAG 5 cut(s) 309, 1564, 2039, 2787, 2915
SmoI CTYRAG 5 cut(s) 309, 1564, 2039, 2787, 2915
SpeI ACTAGT 1 cut(s) 2353
SphI GCATGC 2 cut(s) 1502, 2605
SsiI CCGC 7 cut(s) 623, 638, 2195, 2311, 3537, 3540, 3723
SspI AATATT 1 cut(s) 1242
SspMI CTAG 8 cut(s) 431, 720, 1268, 1515, 2315, 2354, 2931, 2952
SstI GAGCTC 1 cut(s) 2788
StyI CCWWGG 3 cut(s) 544, 687, 3641
TaiI ACGT 2 cut(s) 3270, 3297
TaqI TCGA 9 cut(s) 150, 310, 694, 850, 1647, 1833, 2707, 2782, 3486
TatI WGTACW 6 cut(s) 1064, 1537, 1543, 2099, 2402, 2878
TauI GCSGC 2 cut(s) 2314, 3540
TseFI GTSAC 3 cut(s) 304, 1924, 2461
Tsp45I GTSAC 3 cut(s) 304, 1924, 2461
TspGWI ACGGA 4 cut(s) 1448, 1784, 2703, 3001
Van91I CCANNNNNTGG 1 cut(s) 1244
Vha464I CTTAAG 2 cut(s) 1564, 2915
VneI GTGCAC 1 cut(s) 1140
VpaK11BI GGWCC 6 cut(s) 881, 1124, 1151, 2271, 2339, 3410
XagI CCTNNNNNAGG 3 cut(s) 150, 395, 1660
XbaI TCTAGA 1 cut(s) 1267
XceI RCATGY 3 cut(s) 662, 1502, 2605
XhoI CTCGAG 1 cut(s) 309
XmnI GAANNNNTTC 3 cut(s) 156, 2730, 3759
XspI CTAG 8 cut(s) 431, 720, 1268, 1515, 2315, 2354, 2931, 2952
ZrmI AGTACT 3 cut(s) 1539, 2101, 2404
Zsp2I ATGCAT 2 cut(s) 594, 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.