Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007527

Physical Location & Seq

Genomic Coordinates Forward (+)

8798037 .. 8804625

Gene Structure 6589 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1085300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2331 bp

ATGGCTAATGCCTTAATTCTACTCCTCCTCCCTTTTCTCTCTCTACCTTGCTTCCTCCTCTCTCTCTCTGGCGCCACTACTACTTCAACTCAGATTCCCAAGCATCATGTCGTCTATATGGGAAGTTCATTCTCAAATGGAAATGGAAGAGATAATGGAGCTGATGAATCAGCTTACTTGCAAATGTTGTCATCCATTATTCCAAGCCATGAGATTGAGAGAATTTCTGTAATACACAAGTATAATCATGCTTTTAGAGGATTCTCTGCCATGCTTACAGAAACTGAAGCTTCCACTTTATCAGGCAATGATGGTATTGTTTCCATCTTCCCCGACTCAATACTCCAACTACATACAACACGTTCTTGGGATTTCTTGGATCCGAAATCTGTCAGGGCATGGAAGAATGAGTATCTGCGACCATCAAGTGATGTTATAATTGGGATGATAGACACAGGTATATGGCCTGAGTCTCCGAGTTTCAGTGACAAGGGAATCAGTGCAGTTCCTTCAAGATGGAAAGGAGTCTGTATGGAAGGCTCTGACTTTAAGAAATCTAATTGTAATAGAAAGTTGATAGGTGCAAGATACTATAATGTTTCGCTGACAACAAACGGTAACCAGAGCCATTTAGCCAGTACAAATGGCTCACCAAGAGATTCTTTTGGCCATGGAACTCACACTACATCCATAGCAGCCGGTGTGCAAGTTCCCAATGCAAGTTATTATGGCTTAGCACAAGGTATAGCAAGGGGAGGCTCACCGTCAGCTAGGATTGCGTGTTACAAGGCATGCTCGGATGTTGGTTGCTCTGGTGCCACCATATTGAAGGCTATTGATGATGCAGTTAGAGATGGAGTAGACGTAATATCTATTTCCATTGGGATGAGCTCACTGTTTCAGCCTGACTACTTAAATGACCCCATAGCCATCGGAGCATTTCATGCTGAGCAAATGGGAGTGATGGTAATTTGCTCTGCAGGAAATGATGGTCCTGATCCTTACACAATTGTAAACACAGCACCGTGGATCTTCACCGTTGCAGCTTCTAACATTGATAGGGATTTCCAATCTAATATCGTTCTTGGAAATGGAAGAACATTCACAGGTTCAGCCATCAATTTCTCCAATCTCACTCACTCGAAGACATATCCTCTTGTATTTGGAAAAGATGCCGCTGGCATTTACACGCCTGTATCAGAAGCAAGAAATTGCTATCCAGGATCATTGGATCAAAAGAAAGTTGTTGGCAAAATAGTTGTTTGTGTTGATGATGACCCGGCTGTTTCACGGAGAATCAAGAAATTAGTTGTTGCTGATACTAAAGCCAAAGGGTTGATTTTGATTGATGAAGCTGAGAAAGGTGTACCTTTCGATTCGGGCATTTTCCCATTTACAGAAGTTGGCAATACTGCAGGGTTTCAGATTCTCGAGTACATAAATTCTACCAAAAACCCAACAGCAACGATTCTTCCAACAGTTGTAGTTCCACAGTATAGACCTGCACCGGCTGTTGCATTTTTTTCATCGAGGGGTCCTGCAGAGCTTACAGAAAACATTCTGAAGCCTGATATAATGGCTCCAGGAGTGGCCATTTTAGCTGCCATAGCTCCCAGGAATGAAAAAGGGACAGTCCCAAATGGGAAGAAGCCATCAAAATTTTCCATAAAATCCGGGACATCAATGGCTTGTCCACACGTAACGGGGGCTGCTGCATTCATCAAGTCAGTGCATCATCGATGGACTACTTCAATGATCAAATCAGCCCTTATGACAACAGCAACCGTTTACAACAACATGAAAAAGCCTTTGACAAACAGCTCTAACCACTTTGCAAATCCACACGAGGTGGGGGTCGGAGAAATAAACCCACTTAAAGCTCTTAACCCGGGACTAGTCTTCGAAACCACAACGGAAAACTATCTCGAATTCCTTTGCTATTACGGCTACCCAGAGAAGAACATAAGATTTATGTCAAACACAAAGTTCAACTGCCCTAAAATCTCCACTGAAGAACTCATCTCTAACATCAACTACCCCTCCATCTCCATTAGTAAGCTCAGCTGGCGCCAACCTGCAAAGGCTATTGAAAGAACTGTGACCAATGTTGGAGCCCGGAATTCTACGTACATTGCAAAGGTGCAGGCTCCAGTAGGATTAAAAGTCAAGGTACTTCCGGAGAAGTTGGTTTTTTCTGAGGGTGTGAGGAGAGTGTCTTTCCAAGTGTCATTTTACGGCAAGAAGGCTACCAGCGGCTACAATTTTGGGTCCATAATATGGTTTGATGGTCAACATTCAGTTCGTACTGTGTTTAGTGTGAATGTTGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

777

Amino Acids

83.88

Weight (kDa)

8.93

Isoelectric Point (pI)

32.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	36 - 118	3.2e-12	Peptidase inhibitor I9
Peptidase_S8	PF00082	143 - 605	3.9e-45	Subtilase family
fn3_6	PF17766	676 - 773	3e-24	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007527)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g04630 FvH4_7g04630 FvH4_7g04630 FvH4_7g04630 FvH4_7g04630 FvH4_7g04630
malus_domestica	MD02G1268900.v1.1 MD07G1085300.v1.1
prunus_persica	Prupe.2G051800_v2.0.a1 Prupe.2G051800_v2.0.a1
pyrus_communis	pycom02g23060 pycom07g03800 pycom07g03810
rosa_chinensis	RchiOBHm_Chr1g0330061
rosa_laevigata	RLG00000029837
rosa_multiflora	Rmu_co8353995.1_g000001 Rmu_sc0004673.1_g000001
rosa_roxburghii	Rroxscaffold_4G00320400
rosa_rugosa	Rorug01G0085500 Rorug01G0085600
rosa_samantha	Rh1AG104100 Rh1BG082500 Rh1CG099000 Rh1DG106200
rosa_wichuraiana	Rw0G015920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	437
Acc36I	ACCTGC	2 cut(s)	1510, 2083
AccB1I	GGYRCC	3 cut(s)	71, 815, 2067
AccB7I	CCANNNNNTGG	1 cut(s)	2277
AccI	GTMKAC	1 cut(s)	861
AccIII	TCCGGA	1 cut(s)	2176
AciI	CCGC	2 cut(s)	1176, 2253
AclWI	GGATC	6 cut(s)	374, 387, 992, 1037, 1231, 1239
AcoI	YGGCCR	2 cut(s)	667, 1590
AcsI	RAATTY	5 cut(s)	222, 1441, 1658, 1928, 2119
AcuI	CTGAAG	3 cut(s)	306, 1583, 2031
AcyI	GRCGYC	2 cut(s)	72, 2068
AdeI	CACNNNGTG	1 cut(s)	1849
AfaI	GTAC	6 cut(s)	640, 1368, 1436, 2129, 2172, 2305
AfiI	CCNNNNNNNGG	1 cut(s)	2277
AflIII	ACRYGT	2 cut(s)	359, 1696
AgsI	TTSAA	7 cut(s)	87, 513, 829, 1752, 1990, 2090, 2327
AhlI	ACTAGT	1 cut(s)	1894
AjnI	CCWGG	3 cut(s)	1219, 1582, 1613
Alw21I	GWGCWC	1 cut(s)	893
Alw26I	GTCTC	1 cut(s)	477
AlwI	GGATC	6 cut(s)	374, 387, 992, 1037, 1231, 1239
AlwNI	CAGNNNCTG	1 cut(s)	284
Ama87I	CYCGRG	2 cut(s)	1430, 1888
Aor13HI	TCCGGA	1 cut(s)	2176
AoxI	GGCC	3 cut(s)	464, 667, 1590
ApeKI	GCWGC	5 cut(s)	695, 1043, 1601, 1709, 1712
ApoI	RAATTY	5 cut(s)	222, 1441, 1658, 1928, 2119
Asp700I	GAANNNNTTC	1 cut(s)	1557
AspLEI	GCGC	2 cut(s)	74, 2070
AspS9I	GGNCC	3 cut(s)	992, 1535, 2268
AsuC2I	CCSGG	5 cut(s)	1280, 1675, 1889, 1890, 2116
AsuHPI	GGTGA	3 cut(s)	642, 753, 1027
AsuII	TTCGAA	1 cut(s)	1902
AvaI	CYCGRG	2 cut(s)	1430, 1888
AvaII	GGWCC	3 cut(s)	992, 1535, 2268
BaeI	ACNNNNGTAYC	2 cut(s)	1179, 1212
BalI	TGGCCA	2 cut(s)	669, 1592
BamHI	GGATCC	1 cut(s)	379
BanI	GGYRCC	3 cut(s)	71, 815, 2067
BanII	GRGCYC	2 cut(s)	893, 2116
BauI	CACGAG	1 cut(s)	1844
BbsI	GAAGAC	2 cut(s)	1151, 1891
Bbv12I	GWGCWC	1 cut(s)	893
BbvI	GCAGC	5 cut(s)	707, 1055, 1588, 1696, 1699
BceAI	ACGGC	2 cut(s)	1960, 2251
BciT130I	CCWGG	3 cut(s)	1221, 1584, 1615
BclI	TGATCA	1 cut(s)	1755
BcnI	CCSGG	5 cut(s)	1280, 1675, 1889, 1890, 2116
BcoDI	GTCTC	1 cut(s)	477
BcuI	ACTAGT	1 cut(s)	1894
BfaI	CTAG	2 cut(s)	771, 1895
BfmI	CTRYAG	3 cut(s)	978, 1413, 1539
BfoI	RGCGCY	2 cut(s)	75, 2071
BfuAI	ACCTGC	2 cut(s)	1510, 2083
BisI	GCNGC	7 cut(s)	696, 1044, 1176, 1602, 1710, 1713, 2254
BlpI	GCTNAGC	3 cut(s)	733, 948, 2060
BlsI	GCNGC	7 cut(s)	697, 1045, 1177, 1603, 1711, 1714, 2255
Bme1390I	CCNGG	8 cut(s)	1221, 1280, 1584, 1615, 1675, 1889, 1890, 2116
Bme18I	GGWCC	3 cut(s)	992, 1535, 2268
BmeT110I	CYCGRG	2 cut(s)	1430, 1888
BmgT120I	GGNCC	3 cut(s)	992, 1535, 2268
BmiI	GGNNCC	9 cut(s)	73, 381, 817, 1536, 1581, 2069, 2113, 2148, 2269
BmrFI	CCNGG	8 cut(s)	1221, 1280, 1584, 1615, 1675, 1889, 1890, 2116
BmsI	GCATC	4 cut(s)	112, 832, 1162, 1741
BpiI	GAAGAC	2 cut(s)	1151, 1891
BpmI	CTGGAG	2 cut(s)	1566, 2133
Bpu1102I	GCTNAGC	3 cut(s)	733, 948, 2060
Bpu14I	TTCGAA	1 cut(s)	1902
BpuMI	CCSGG	5 cut(s)	1280, 1675, 1889, 1890, 2116
Bsa29I	ATCGAT	1 cut(s)	1738
BsaAI	YACGTR	2 cut(s)	1699, 2127
BsaHI	GRCGYC	2 cut(s)	72, 2068
BsaJI	CCNNGG	4 cut(s)	670, 1025, 1613, 1888
BsaWI	WCCGGW	1 cut(s)	2176
BsaXI	ACNNNNNCTCC	8 cut(s)	12, 42, 849, 879, 951, 981, 2024, 2054
Bsc4I	CCNNNNNNNGG	1 cut(s)	2277
Bse118I	RCCGGY	2 cut(s)	698, 1507
Bse1I	ACTGG	2 cut(s)	636, 2150
Bse3DI	GCAATG	2 cut(s)	313, 2130
BseAI	TCCGGA	1 cut(s)	2176
BseBI	CCWGG	3 cut(s)	1221, 1584, 1615
BseCI	ATCGAT	1 cut(s)	1738
BseDI	CCNNGG	4 cut(s)	670, 1025, 1613, 1888
BseGI	GGATG	5 cut(s)	191, 450, 686, 805, 891
BseLI	CCNNNNNNNGG	1 cut(s)	2277
BseMI	GCAATG	2 cut(s)	313, 2130
BseMII	CTCAG	6 cut(s)	104, 459, 939, 1347, 2074, 2187
BseNI	ACTGG	2 cut(s)	636, 2150
BseRI	GAGGAG	4 cut(s)	14, 17, 47, 2221
BseXI	GCAGC	5 cut(s)	707, 1055, 1588, 1696, 1699
BsgI	GTGCAG	3 cut(s)	522, 1488, 2162
BshFI	GGCC	3 cut(s)	466, 669, 1592
BshNI	GGYRCC	3 cut(s)	71, 815, 2067
BshVI	ATCGAT	1 cut(s)	1738
BsiHKAI	GWGCWC	1 cut(s)	893
BsiHKCI	CYCGRG	2 cut(s)	1430, 1888
BsiSI	CCGG	7 cut(s)	699, 1280, 1508, 1674, 1889, 2116, 2177
BslFI	GGGAC	4 cut(s)	1619, 1642, 1690, 1905
BslI	CCNNNNNNNGG	1 cut(s)	2277
BsmAI	GTCTC	1 cut(s)	477
BsmFI	GGGAC	4 cut(s)	1619, 1642, 1690, 1905
BsmI	GAATGC	1 cut(s)	1715
BsnI	GGCC	3 cut(s)	466, 669, 1592
BsoBI	CYCGRG	2 cut(s)	1430, 1888
Bsp119I	TTCGAA	1 cut(s)	1902
Bsp1286I	GDGCHC	2 cut(s)	893, 2116
Bsp13I	TCCGGA	1 cut(s)	2176
Bsp143I	GATC	6 cut(s)	379, 997, 1029, 1223, 1231, 1755
Bsp1720I	GCTNAGC	3 cut(s)	733, 948, 2060
Bsp19I	CCATGG	1 cut(s)	670
BspACI	CCGC	2 cut(s)	1176, 2253
BspANI	GGCC	3 cut(s)	466, 669, 1592
BspCNI	CTCAG	6 cut(s)	103, 460, 940, 1348, 2073, 2188
BspDI	ATCGAT	1 cut(s)	1738
BspEI	TCCGGA	1 cut(s)	2176
BspLI	GGNNCC	9 cut(s)	73, 381, 817, 1536, 1581, 2069, 2113, 2148, 2269
BspMAI	CTGCAG	3 cut(s)	982, 1417, 1543
BspMI	ACCTGC	2 cut(s)	1510, 2083
BspPI	GGATC	6 cut(s)	374, 387, 992, 1037, 1231, 1239
BspT104I	TTCGAA	1 cut(s)	1902
BspT107I	GGYRCC	3 cut(s)	71, 815, 2067
BsrDI	GCAATG	2 cut(s)	313, 2130
BsrFI	RCCGGY	2 cut(s)	698, 1507
BsrI	ACTGG	2 cut(s)	636, 2150
BssAI	RCCGGY	2 cut(s)	698, 1507
BssECI	CCNNGG	4 cut(s)	670, 1025, 1613, 1888
BssMI	GATC	6 cut(s)	379, 997, 1029, 1223, 1231, 1755
BssNI	GRCGYC	2 cut(s)	72, 2068
BssSI	CACGAG	1 cut(s)	1844
BssT1I	CCWWGG	1 cut(s)	670
Bst2BI	CACGAG	1 cut(s)	1844
Bst2UI	CCWGG	3 cut(s)	1221, 1584, 1615
Bst6I	CTCTTC	1 cut(s)	142
BstACI	GRCGYC	2 cut(s)	72, 2068
BstAPI	GCANNNNNTGC	1 cut(s)	944
BstBAI	YACGTR	2 cut(s)	1699, 2127
BstBI	TTCGAA	1 cut(s)	1902
BstC8I	GCNNGC	4 cut(s)	793, 1180, 2066, 2145
BstDEI	CTNAG	7 cut(s)	90, 468, 733, 948, 1356, 2060, 2196
BstDSI	CCRYGG	2 cut(s)	670, 1025
BstEII	GGTNACC	1 cut(s)	617
BstF5I	GGATG	5 cut(s)	191, 450, 686, 805, 891
BstH2I	RGCGCY	2 cut(s)	75, 2071
BstHHI	GCGC	2 cut(s)	74, 2070
BstKTI	GATC	6 cut(s)	382, 1000, 1032, 1226, 1234, 1758
BstMAI	GTCTC	1 cut(s)	477
BstMBI	GATC	6 cut(s)	379, 997, 1029, 1223, 1231, 1755
BstMWI	GCNNNNNNNGC	7 cut(s)	776, 935, 944, 1598, 1607, 1944, 2065
BstNI	CCWGG	3 cut(s)	1221, 1584, 1615
BstNSI	RCATGY	1 cut(s)	795
BstPI	GGTNACC	1 cut(s)	617
BstSCI	CCNGG	8 cut(s)	1219, 1278, 1582, 1613, 1673, 1887, 1888, 2114
BstSFI	CTRYAG	3 cut(s)	978, 1413, 1539
BstSNI	TACGTA	1 cut(s)	2127
BstV1I	GCAGC	5 cut(s)	707, 1055, 1588, 1696, 1699
BstV2I	GAAGAC	2 cut(s)	1151, 1891
BstX2I	RGATCY	2 cut(s)	379, 1029
BstYI	RGATCY	2 cut(s)	379, 1029
Bsu15I	ATCGAT	1 cut(s)	1738
BsuRI	GGCC	3 cut(s)	466, 669, 1592
BsuTUI	ATCGAT	1 cut(s)	1738
BtgI	CCRYGG	2 cut(s)	670, 1025
BtsCI	GGATG	5 cut(s)	191, 450, 686, 805, 891
BtsIMutI	CAGTG	5 cut(s)	490, 505, 893, 1734, 2007
BveI	ACCTGC	2 cut(s)	1510, 2083
Cac8I	GCNNGC	4 cut(s)	793, 1180, 2066, 2145
CaiI	CAGNNNCTG	1 cut(s)	284
CfoI	GCGC	2 cut(s)	74, 2070
Cfr10I	RCCGGY	2 cut(s)	698, 1507
Cfr13I	GGNCC	3 cut(s)	992, 1535, 2268
Cfr9I	CCCGGG	1 cut(s)	1888
ClaI	ATCGAT	1 cut(s)	1738
Csp6I	GTAC	6 cut(s)	639, 1367, 1435, 2128, 2171, 2304
CviAII	CATG	9 cut(s)	107, 209, 248, 271, 399, 671, 792, 944, 1798
CviQI	GTAC	6 cut(s)	639, 1367, 1435, 2128, 2171, 2304
DdeI	CTNAG	7 cut(s)	90, 468, 733, 948, 1356, 2060, 2196
DinI	GGCGCC	2 cut(s)	73, 2069
DpnI	GATC	6 cut(s)	381, 999, 1031, 1225, 1233, 1757
DpnII	GATC	6 cut(s)	379, 997, 1029, 1223, 1231, 1755
DraIII	CACNNNGTG	1 cut(s)	1849
EaeI	YGGCCR	2 cut(s)	667, 1590
Eam1104I	CTCTTC	1 cut(s)	142
EarI	CTCTTC	1 cut(s)	142
Ecl136II	GAGCTC	1 cut(s)	891
Eco105I	TACGTA	1 cut(s)	2127
Eco130I	CCWWGG	1 cut(s)	670
Eco24I	GRGCYC	2 cut(s)	893, 2116
Eco47I	GGWCC	3 cut(s)	992, 1535, 2268
Eco53kI	GAGCTC	1 cut(s)	891
Eco57I	CTGAAG	3 cut(s)	306, 1583, 2031
Eco88I	CYCGRG	2 cut(s)	1430, 1888
Eco91I	GGTNACC	1 cut(s)	617
EcoICRI	GAGCTC	1 cut(s)	891
EcoO109I	RGGNCCY	1 cut(s)	1535
EcoO65I	GGTNACC	1 cut(s)	617
EcoRI	GAATTC	2 cut(s)	1928, 2119
EcoRII	CCWGG	3 cut(s)	1219, 1582, 1613
EcoT14I	CCWWGG	1 cut(s)	670
EcoT38I	GRGCYC	2 cut(s)	893, 2116
EgeI	GGCGCC	2 cut(s)	73, 2069
EheI	GGCGCC	2 cut(s)	73, 2069
ErhI	CCWWGG	1 cut(s)	670
FaeI	CATG	9 cut(s)	110, 212, 251, 274, 402, 674, 795, 947, 1801
FalI	AAGNNNNNCTT	2 cut(s)	646, 678
FaqI	GGGAC	4 cut(s)	1619, 1642, 1690, 1905
FatI	CATG	9 cut(s)	106, 208, 247, 270, 398, 670, 791, 943, 1797
FbaI	TGATCA	1 cut(s)	1755
FblI	GTMKAC	1 cut(s)	861
Fnu4HI	GCNGC	7 cut(s)	696, 1044, 1176, 1602, 1710, 1713, 2254
FokI	GGATG	5 cut(s)	178, 457, 673, 812, 898
FriOI	GRGCYC	2 cut(s)	893, 2116
Fsp4HI	GCNGC	7 cut(s)	696, 1044, 1176, 1602, 1710, 1713, 2254
FspBI	CTAG	2 cut(s)	771, 1895
GlaI	GCGC	2 cut(s)	73, 2069
GluI	GCNGC	7 cut(s)	696, 1044, 1176, 1602, 1710, 1713, 2254
GsuI	CTGGAG	2 cut(s)	1566, 2133
HaeII	RGCGCY	2 cut(s)	75, 2071
HaeIII	GGCC	3 cut(s)	466, 669, 1592
HapII	CCGG	7 cut(s)	699, 1280, 1508, 1674, 1889, 2116, 2177
HhaI	GCGC	2 cut(s)	74, 2070
Hin1I	GRCGYC	2 cut(s)	72, 2068
Hin1II	CATG	9 cut(s)	110, 212, 251, 274, 402, 674, 795, 947, 1801
Hin6I	GCGC	2 cut(s)	72, 2068
HinP1I	GCGC	2 cut(s)	72, 2068
HincII	GTYRAC	1 cut(s)	2291
HindII	GTYRAC	1 cut(s)	2291
HindIII	AAGCTT	1 cut(s)	288
HpaII	CCGG	7 cut(s)	699, 1280, 1508, 1674, 1889, 2116, 2177
HphI	GGTGA	3 cut(s)	642, 753, 1027
Hpy166II	GTNNAC	6 cut(s)	862, 1015, 1367, 1694, 1789, 2291
Hpy188III	TCNNGA	6 cut(s)	513, 995, 1300, 1430, 1925, 2177
Hpy8I	GTNNAC	6 cut(s)	862, 1015, 1367, 1694, 1789, 2291
HpyAV	CCTTC	4 cut(s)	519, 530, 823, 2236
HpyCH4IV	ACGT	4 cut(s)	361, 864, 1698, 2126
HpyF10VI	GCNNNNNNNGC	7 cut(s)	776, 935, 944, 1598, 1607, 1944, 2065
HpyF3I	CTNAG	7 cut(s)	90, 468, 733, 948, 1356, 2060, 2196
HpySE526I	ACGT	4 cut(s)	361, 864, 1698, 2126
Hsp92I	GRCGYC	2 cut(s)	72, 2068
Hsp92II	CATG	9 cut(s)	110, 212, 251, 274, 402, 674, 795, 947, 1801
HspAI	GCGC	2 cut(s)	72, 2068
KasI	GGCGCC	2 cut(s)	71, 2067
Kpn2I	TCCGGA	1 cut(s)	2176
Ksp22I	TGATCA	1 cut(s)	1755
Kzo9I	GATC	6 cut(s)	379, 997, 1029, 1223, 1231, 1755
LmnI	GCTCC	6 cut(s)	158, 935, 1585, 1615, 2111, 2152
Lsp1109I	GCAGC	5 cut(s)	707, 1055, 1588, 1696, 1699
LweI	GCATC	4 cut(s)	112, 832, 1162, 1741
MaeI	CTAG	2 cut(s)	771, 1895
MaeII	ACGT	4 cut(s)	361, 864, 1698, 2126
MaeIII	GTNAC	5 cut(s)	485, 617, 782, 1699, 2098
MalI	GATC	6 cut(s)	381, 999, 1031, 1225, 1233, 1757
MboI	GATC	6 cut(s)	379, 997, 1029, 1223, 1231, 1755
MfeI	CAATTG	1 cut(s)	1008
MflI	RGATCY	2 cut(s)	379, 1029
MhlI	GDGCHC	2 cut(s)	893, 2116
MlsI	TGGCCA	2 cut(s)	669, 1592
MluNI	TGGCCA	2 cut(s)	669, 1592
Mly113I	GGCGCC	2 cut(s)	72, 2068
MlyI	GAGTC	3 cut(s)	329, 479, 534
MmeI	TCCRAC	4 cut(s)	370, 1499, 1837, 2089
Mox20I	TGGCCA	2 cut(s)	669, 1592
MroI	TCCGGA	1 cut(s)	2176
MroXI	GAANNNNTTC	1 cut(s)	1557
MscI	TGGCCA	2 cut(s)	669, 1592
MseI	TTAA	6 cut(s)	14, 549, 914, 1875, 1884, 2159
MslI	CAYNNNNRTG	1 cut(s)	884
Msp20I	TGGCCA	2 cut(s)	669, 1592
MspA1I	CMGCKG	3 cut(s)	1178, 2064, 2253
MspI	CCGG	7 cut(s)	699, 1280, 1508, 1674, 1889, 2116, 2177
MspR9I	CCNGG	8 cut(s)	1221, 1280, 1584, 1615, 1675, 1889, 1890, 2116
MunI	CAATTG	1 cut(s)	1008
Mva1269I	GAATGC	1 cut(s)	1715
MvaI	CCWGG	3 cut(s)	1221, 1584, 1615
MwoI	GCNNNNNNNGC	7 cut(s)	776, 935, 944, 1598, 1607, 1944, 2065
NarI	GGCGCC	2 cut(s)	72, 2068
NciI	CCSGG	5 cut(s)	1280, 1675, 1889, 1890, 2116
NcoI	CCATGG	1 cut(s)	670
NdeII	GATC	6 cut(s)	379, 997, 1029, 1223, 1231, 1755
NlaIII	CATG	9 cut(s)	110, 212, 251, 274, 402, 674, 795, 947, 1801
NlaIV	GGNNCC	9 cut(s)	73, 381, 817, 1536, 1581, 2069, 2113, 2148, 2269
NmuCI	GTSAC	2 cut(s)	485, 2098
NspI	RCATGY	1 cut(s)	795
NspV	TTCGAA	1 cut(s)	1902
PaeI	GCATGC	1 cut(s)	795
PaeR7I	CTCGAG	1 cut(s)	1430
PctI	GAATGC	1 cut(s)	1715
PdmI	GAANNNNTTC	1 cut(s)	1557
PfeI	GAWTC	9 cut(s)	94, 167, 261, 495, 659, 1296, 1376, 1426, 1468
PflMI	CCANNNNNTGG	1 cut(s)	2277
PfoI	TCCNGGA	3 cut(s)	1219, 1582, 1673
PkrI	GCNGC	7 cut(s)	697, 1045, 1177, 1603, 1711, 1714, 2255
PleI	GAGTC	3 cut(s)	329, 478, 533
PluTI	GGCGCC	2 cut(s)	75, 2071
PpsI	GAGTC	3 cut(s)	329, 478, 533
Ppu21I	YACGTR	2 cut(s)	1699, 2127
PpuMI	RGGWCCY	1 cut(s)	1535
PsiI	TTATAA	1 cut(s)	437
Psp124BI	GAGCTC	1 cut(s)	893
Psp5II	RGGWCCY	1 cut(s)	1535
Psp6I	CCWGG	3 cut(s)	1219, 1582, 1613
PspEI	GGTNACC	1 cut(s)	617
PspGI	CCWGG	3 cut(s)	1219, 1582, 1613
PspN4I	GGNNCC	9 cut(s)	73, 381, 817, 1536, 1581, 2069, 2113, 2148, 2269
PspPI	GGNCC	3 cut(s)	992, 1535, 2268
PspPPI	RGGWCCY	1 cut(s)	1535
PsrI	GAACNNNNNNTAC	2 cut(s)	667, 699
PstI	CTGCAG	3 cut(s)	982, 1417, 1543
PstNI	CAGNNNCTG	1 cut(s)	284
PsuI	RGATCY	2 cut(s)	379, 1029
PvuII	CAGCTG	1 cut(s)	2064
RsaI	GTAC	6 cut(s)	640, 1368, 1436, 2129, 2172, 2305
RsaNI	GTAC	6 cut(s)	639, 1367, 1435, 2128, 2171, 2304
RseI	CAYNNNNRTG	1 cut(s)	884
SacI	GAGCTC	1 cut(s)	893
SaqAI	TTAA	6 cut(s)	14, 549, 914, 1875, 1884, 2159
SatI	GCNGC	7 cut(s)	696, 1044, 1176, 1602, 1710, 1713, 2254
Sau3AI	GATC	6 cut(s)	379, 997, 1029, 1223, 1231, 1755
Sau96I	GGNCC	3 cut(s)	992, 1535, 2268
SchI	GAGTC	3 cut(s)	329, 479, 534
ScrFI	CCNGG	8 cut(s)	1221, 1280, 1584, 1615, 1675, 1889, 1890, 2116
SduI	GDGCHC	2 cut(s)	893, 2116
SfaNI	GCATC	4 cut(s)	112, 832, 1162, 1741
SfcI	CTRYAG	3 cut(s)	978, 1413, 1539
SfoI	GGCGCC	2 cut(s)	73, 2069
Sfr274I	CTCGAG	1 cut(s)	1430
SfuI	TTCGAA	1 cut(s)	1902
SinI	GGWCC	3 cut(s)	992, 1535, 2268
SlaI	CTCGAG	1 cut(s)	1430
SmaI	CCCGGG	1 cut(s)	1890
SmiMI	CAYNNNNRTG	1 cut(s)	884
SmlI	CTYRAG	1 cut(s)	1430
SmoI	CTYRAG	1 cut(s)	1430
SnaBI	TACGTA	1 cut(s)	2127
SpeI	ACTAGT	1 cut(s)	1894
SphI	GCATGC	1 cut(s)	795
SsiI	CCGC	2 cut(s)	1176, 2253
SspDI	GGCGCC	2 cut(s)	71, 2067
SspMI	CTAG	2 cut(s)	771, 1895
SstI	GAGCTC	1 cut(s)	893
StyD4I	CCNGG	8 cut(s)	1219, 1278, 1582, 1613, 1673, 1887, 1888, 2114
StyI	CCWWGG	1 cut(s)	670
TaiI	ACGT	4 cut(s)	364, 867, 1701, 2129
TaqI	TCGA	7 cut(s)	1142, 1374, 1431, 1529, 1738, 1902, 1926
TatI	WGTACW	2 cut(s)	638, 1434
TauI	GCSGC	2 cut(s)	1178, 2256
TfiI	GAWTC	9 cut(s)	94, 167, 261, 495, 659, 1296, 1376, 1426, 1468
Tru1I	TTAA	6 cut(s)	14, 549, 914, 1875, 1884, 2159
Tru9I	TTAA	6 cut(s)	14, 549, 914, 1875, 1884, 2159
TscAI	CASTG	5 cut(s)	490, 505, 900, 1734, 2014
TseFI	GTSAC	2 cut(s)	485, 2098
TseI	GCWGC	5 cut(s)	695, 1043, 1601, 1709, 1712
Tsp45I	GTSAC	2 cut(s)	485, 2098
TspDTI	ATGAA	8 cut(s)	117, 180, 932, 1365, 1515, 1635, 1708, 1814
TspGWI	ACGGA	2 cut(s)	1306, 1928
TspMI	CCCGGG	1 cut(s)	1888
TspRI	CASTG	5 cut(s)	490, 505, 900, 1734, 2014
Van91I	CCANNNNNTGG	1 cut(s)	2277
VpaK11BI	GGWCC	3 cut(s)	992, 1535, 2268
XapI	RAATTY	5 cut(s)	222, 1441, 1658, 1928, 2119
XceI	RCATGY	1 cut(s)	795
XhoI	CTCGAG	1 cut(s)	1430
XmaI	CCCGGG	1 cut(s)	1888
XmiI	GTMKAC	1 cut(s)	861
XmnI	GAANNNNTTC	1 cut(s)	1557
XspI	CTAG	2 cut(s)	771, 1895

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.