Rmu_co8353995.1_g000001

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8353995.1
OG0007527
Physical Location & Seq
Forward (+)
1 .. 1053
1053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8353995.1_g000001.1.cds

Sequence Viewer

Length: 666 bp
gtatatggcccgagtctccaagtttcaatgatgagggaattggtgcagtcccttcaagatggaaaggggtctgtatggagggcccggacttcaagaaatctaactgtaacagaaagttgataggtgcaagatattacaatatgcaaatgacaacgattggtaaccagagccatttagctgcatcaaaaggctcaccaagagattttcttggccacgggactcacactgcatccacagctgcgggtgcacgagttcccaatgctagttactatggcttggcgcaaggtacagcaaagggaggcttgccatcagctaggattgcaagctacaaggcatgctcagatgtcggttgttctggtgccaccctattgaaggccattgatgatgcaattagagatggagtagacatgatatctatttccattggggtgagctcactatttcaacctgactacctaagtgatcccatagccattggaacatttcatgctgaacaaatgggagtcatagtcatttgctctggaggaaatgatggtcctgatccttacactgttgttaacacagcgccgtggatcttcactgttgcagcttctaacattgatagggatttccaatctactgtagtccttggaaatgggagaactttcacggttagttacttaatca
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

221

Amino Acids

23.29

Weight (kDa)

6.45

Isoelectric Point (pI)

25.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 358
AccI GTMKAC 1 cut(s) 404
AciI CCGC 1 cut(s) 241
AclWI GGATC 3 cut(s) 457, 535, 580
AcoI YGGCCR 1 cut(s) 210
AfaI GTAC 1 cut(s) 288
AfiI CCNNNNNNNGG 1 cut(s) 372
AgsI TTSAA 5 cut(s) 27, 56, 93, 372, 445
AluBI AGCT 6 cut(s) 178, 238, 313, 326, 434, 589
AluI AGCT 6 cut(s) 178, 238, 313, 326, 434, 589
Alw21I GWGCWC 2 cut(s) 249, 436
Alw26I GTCTC 1 cut(s) 20
Alw44I GTGCAC 1 cut(s) 245
AlwI GGATC 3 cut(s) 457, 535, 580
Ama87I CYCGRG 1 cut(s) 10
AoxI GGCC 4 cut(s) 7, 81, 210, 374
ApaI GGGCCC 1 cut(s) 85
ApaLI GTGCAC 1 cut(s) 245
ApeKI GCWGC 3 cut(s) 178, 238, 586
AspLEI GCGC 2 cut(s) 282, 567
AspS9I GGNCC 4 cut(s) 8, 81, 82, 535
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 2 cut(s) 185, 441
AvaI CYCGRG 1 cut(s) 10
AvaII GGWCC 1 cut(s) 535
BaeGI GKGCMC 2 cut(s) 85, 249
BalI TGGCCA 1 cut(s) 212
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 2 cut(s) 85, 436
BauI CACGAG 1 cut(s) 248
Bbv12I GWGCWC 2 cut(s) 249, 436
BbvI GCAGC 3 cut(s) 165, 225, 598
BccI CCATC 4 cut(s) 53, 315, 391, 526
BceAI ACGGC 1 cut(s) 552
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 2 cut(s) 263, 314
BfmI CTRYAG 1 cut(s) 620
BfoI RGCGCY 1 cut(s) 568
BisI GCNGC 3 cut(s) 179, 239, 587
BlsI GCNGC 3 cut(s) 180, 240, 588
Bme1390I CCNGG 1 cut(s) 85
Bme18I GGWCC 1 cut(s) 535
BmeT110I CYCGRG 1 cut(s) 10
BmgT120I GGNCC 4 cut(s) 8, 81, 82, 535
BmiI GGNNCC 2 cut(s) 83, 360
BmrFI CCNGG 1 cut(s) 85
BmsI GCATC 3 cut(s) 190, 238, 375
BpmI CTGGAG 1 cut(s) 542
BpuMI CCSGG 1 cut(s) 85
BsaJI CCNNGG 3 cut(s) 213, 568, 627
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 1 cut(s) 372
BseDI CCNNGG 3 cut(s) 213, 568, 627
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 353
BseSI GKGCMC 2 cut(s) 85, 249
BseXI GCAGC 3 cut(s) 165, 225, 598
BsgI GTGCAG 1 cut(s) 65
BshFI GGCC 4 cut(s) 9, 83, 212, 376
BshNI GGYRCC 1 cut(s) 358
BsiHKAI GWGCWC 2 cut(s) 249, 436
BsiHKCI CYCGRG 1 cut(s) 10
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 2 cut(s) 34, 231
BslI CCNNNNNNNGG 1 cut(s) 372
BsmAI GTCTC 1 cut(s) 20
BsmFI GGGAC 2 cut(s) 34, 231
BsnI GGCC 4 cut(s) 9, 83, 212, 376
BsoBI CYCGRG 1 cut(s) 10
Bsp120I GGGCCC 1 cut(s) 81
Bsp1286I GDGCHC 3 cut(s) 85, 249, 436
Bsp143I GATC 3 cut(s) 462, 540, 572
BspACI CCGC 1 cut(s) 241
BspANI GGCC 4 cut(s) 9, 83, 212, 376
BspCNI CTCAG 1 cut(s) 352
BspLI GGNNCC 2 cut(s) 83, 360
BspPI GGATC 3 cut(s) 457, 535, 580
BspT107I GGYRCC 1 cut(s) 358
BssECI CCNNGG 3 cut(s) 213, 568, 627
BssMI GATC 3 cut(s) 462, 540, 572
BssSI CACGAG 1 cut(s) 248
BssT1I CCWWGG 1 cut(s) 627
Bst2BI CACGAG 1 cut(s) 248
Bst4CI ACNGT 5 cut(s) 106, 552, 582, 621, 651
BstC8I GCNNGC 3 cut(s) 304, 324, 336
BstDEI CTNAG 2 cut(s) 339, 457
BstDSI CCRYGG 2 cut(s) 213, 568
BstEII GGTNACC 1 cut(s) 160
BstENI CCTNNNNNAGG 1 cut(s) 370
BstF5I GGATG 1 cut(s) 229
BstH2I RGCGCY 1 cut(s) 568
BstHHI GCGC 2 cut(s) 282, 567
BstKTI GATC 3 cut(s) 465, 543, 575
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 3 cut(s) 462, 540, 572
BstMWI GCNNNNNNNGC 3 cut(s) 235, 244, 319
BstNSI RCATGY 1 cut(s) 338
BstPI GGTNACC 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 83
BstSFI CTRYAG 1 cut(s) 620
BstSLI GKGCMC 2 cut(s) 85, 249
BstV1I GCAGC 3 cut(s) 165, 225, 598
BstX2I RGATCY 1 cut(s) 572
BstYI RGATCY 1 cut(s) 572
BsuRI GGCC 4 cut(s) 9, 83, 212, 376
BtgI CCRYGG 2 cut(s) 213, 568
BtsCI GGATG 1 cut(s) 229
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 3 cut(s) 224, 548, 578
Cac8I GCNNGC 3 cut(s) 304, 324, 336
CfoI GCGC 2 cut(s) 282, 567
Cfr13I GGNCC 4 cut(s) 8, 81, 82, 535
Csp6I GTAC 1 cut(s) 287
CviAII CATG 3 cut(s) 335, 408, 487
CviQI GTAC 1 cut(s) 287
DdeI CTNAG 2 cut(s) 339, 457
DpnI GATC 3 cut(s) 464, 542, 574
DpnII GATC 3 cut(s) 462, 540, 572
EaeI YGGCCR 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 434
Eco130I CCWWGG 1 cut(s) 627
Eco24I GRGCYC 2 cut(s) 85, 436
Eco32I GATATC 1 cut(s) 413
Eco47I GGWCC 1 cut(s) 535
Eco53kI GAGCTC 1 cut(s) 434
Eco88I CYCGRG 1 cut(s) 10
Eco91I GGTNACC 1 cut(s) 160
EcoICRI GAGCTC 1 cut(s) 434
EcoNI CCTNNNNNAGG 1 cut(s) 370
EcoO109I RGGNCCY 1 cut(s) 81
EcoO65I GGTNACC 1 cut(s) 160
EcoRV GATATC 1 cut(s) 413
EcoT14I CCWWGG 1 cut(s) 627
EcoT38I GRGCYC 2 cut(s) 85, 436
ErhI CCWWGG 1 cut(s) 627
FaeI CATG 3 cut(s) 338, 411, 490
FalI AAGNNNNNCTT 2 cut(s) 286, 318
FaqI GGGAC 2 cut(s) 34, 231
FatI CATG 3 cut(s) 334, 407, 486
FauI CCCGC 1 cut(s) 234
FblI GTMKAC 1 cut(s) 404
Fnu4HI GCNGC 3 cut(s) 179, 239, 587
FokI GGATG 1 cut(s) 216
FriOI GRGCYC 2 cut(s) 85, 436
Fsp4HI GCNGC 3 cut(s) 179, 239, 587
FspBI CTAG 2 cut(s) 263, 314
GlaI GCGC 2 cut(s) 281, 566
GluI GCNGC 3 cut(s) 179, 239, 587
GsuI CTGGAG 1 cut(s) 542
HaeII RGCGCY 1 cut(s) 568
HaeIII GGCC 4 cut(s) 9, 83, 212, 376
HapII CCGG 1 cut(s) 85
HhaI GCGC 2 cut(s) 282, 567
Hin1II CATG 3 cut(s) 338, 411, 490
Hin6I GCGC 2 cut(s) 280, 565
HinP1I GCGC 2 cut(s) 280, 565
HincII GTYRAC 1 cut(s) 558
HindII GTYRAC 1 cut(s) 558
HinfI GANTC 3 cut(s) 13, 219, 503
HpaI GTTAAC 1 cut(s) 558
HpaII CCGG 1 cut(s) 85
HphI GGTGA 2 cut(s) 185, 441
Hpy166II GTNNAC 3 cut(s) 247, 405, 558
Hpy188I TCNGA 1 cut(s) 342
Hpy188III TCNNGA 4 cut(s) 56, 93, 521, 538
Hpy8I GTNNAC 3 cut(s) 247, 405, 558
HpyAV CCTTC 2 cut(s) 62, 366
HpyCH4III ACNGT 5 cut(s) 106, 552, 582, 621, 651
HpyCH4V TGCA 9 cut(s) 46, 127, 144, 181, 229, 247, 322, 388, 586
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 244, 319
HpyF3I CTNAG 2 cut(s) 339, 457
Hsp92II CATG 3 cut(s) 338, 411, 490
HspAI GCGC 2 cut(s) 280, 565
KspAI GTTAAC 1 cut(s) 558
Kzo9I GATC 3 cut(s) 462, 540, 572
LpnPI CCDG 6 cut(s) 98, 178, 341, 461, 506, 551
Lsp1109I GCAGC 3 cut(s) 165, 225, 598
LweI GCATC 3 cut(s) 190, 238, 375
MaeI CTAG 2 cut(s) 263, 314
MaeIII GTNAC 4 cut(s) 106, 160, 265, 655
MalI GATC 3 cut(s) 464, 542, 574
MboI GATC 3 cut(s) 462, 540, 572
MboII GAAGA 1 cut(s) 567
MflI RGATCY 1 cut(s) 572
MhlI GDGCHC 3 cut(s) 85, 249, 436
MlsI TGGCCA 1 cut(s) 212
MluCI AATT 2 cut(s) 38, 389
MluNI TGGCCA 1 cut(s) 212
MlyI GAGTC 3 cut(s) 22, 213, 512
MnlI CCTC 4 cut(s) 27, 72, 292, 517
Mox20I TGGCCA 1 cut(s) 212
MscI TGGCCA 1 cut(s) 212
MseI TTAA 2 cut(s) 557, 661
MslI CAYNNNNRTG 1 cut(s) 427
Msp20I TGGCCA 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 238
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 1 cut(s) 85
MwoI GCNNNNNNNGC 3 cut(s) 235, 244, 319
NciI CCSGG 1 cut(s) 85
NdeII GATC 3 cut(s) 462, 540, 572
NlaIII CATG 3 cut(s) 338, 411, 490
NlaIV GGNNCC 2 cut(s) 83, 360
NspI RCATGY 1 cut(s) 338
PaeI GCATGC 1 cut(s) 338
PkrI GCNGC 3 cut(s) 180, 240, 588
PleI GAGTC 3 cut(s) 21, 213, 511
PpsI GAGTC 3 cut(s) 21, 213, 511
Psp124BI GAGCTC 1 cut(s) 436
PspEI GGTNACC 1 cut(s) 160
PspN4I GGNNCC 2 cut(s) 83, 360
PspOMI GGGCCC 1 cut(s) 81
PspPI GGNCC 4 cut(s) 8, 81, 82, 535
PsuI RGATCY 1 cut(s) 572
PvuII CAGCTG 1 cut(s) 238
RsaI GTAC 1 cut(s) 288
RsaNI GTAC 1 cut(s) 287
RseI CAYNNNNRTG 1 cut(s) 427
SacI GAGCTC 1 cut(s) 436
SaqAI TTAA 2 cut(s) 557, 661
SatI GCNGC 3 cut(s) 179, 239, 587
Sau3AI GATC 3 cut(s) 462, 540, 572
Sau96I GGNCC 4 cut(s) 8, 81, 82, 535
SchI GAGTC 3 cut(s) 22, 213, 512
ScrFI CCNGG 1 cut(s) 85
SduI GDGCHC 3 cut(s) 85, 249, 436
SfaNI GCATC 3 cut(s) 190, 238, 375
SfcI CTRYAG 1 cut(s) 620
SinI GGWCC 1 cut(s) 535
SmiMI CAYNNNNRTG 1 cut(s) 427
SphI GCATGC 1 cut(s) 338
Sse9I AATT 2 cut(s) 38, 389
SsiI CCGC 1 cut(s) 241
SspMI CTAG 2 cut(s) 263, 314
SstI GAGCTC 1 cut(s) 436
StyD4I CCNGG 1 cut(s) 83
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 5 cut(s) 106, 552, 582, 621, 651
TasI AATT 2 cut(s) 38, 389
Tru1I TTAA 2 cut(s) 557, 661
Tru9I TTAA 2 cut(s) 557, 661
TscAI CASTG 3 cut(s) 231, 555, 585
TseI GCWGC 3 cut(s) 178, 238, 586
TspDTI ATGAA 1 cut(s) 475
TspRI CASTG 3 cut(s) 231, 555, 585
VneI GTGCAC 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 535
XagI CCTNNNNNAGG 1 cut(s) 370
XceI RCATGY 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 404
XspI CTAG 2 cut(s) 263, 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.