Rorug01G0085500

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007527
Physical Location & Seq
Reverse (-)
13911394 .. 13912137
744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0085500.1

Sequence Viewer

Length: 744 bp
ATGGAACCACAAACCCAGGAACCAACCAAGGATGGAGGAAGCAACAAAGCAGCCGGGAGTAGTGCTAACATGCTTTGTAGGCGGAACAGGTGGAATCCCACTACAGATCAGATAAGAATCCTCAAGGAGCTTTTCTACAATAAGGGAGTTAGGTCCCCAAGTATAGAGCAGATTCAGAGGATCTGTCTCCAGCTGAAATGGTACGGCAAGATCGAGTTCAAGAACGTCTATTTTTGGTTCGTGAACCAAAGGGCTCGGGAGAAGCAGAAGAAGAAGTCCACTTCGGATGTTCATGTGCCCATGCAAAGATCAGGGCTTGTTGGTGATGACAATGTTACCAATTGGAAACATGAGGATCAGTATATTAACTTTGGATCTTCTGCACCTGCTTCTGCTTCTTCCGCTGGTGTGATGATTGCTGTTAACGGGCAGATGGGGAACTATGGTGGTTATGGATCTATGAACATGGAGAAGAGTGCTAGGGATTGTTCAATCTTAGCTGGAGGGGGAACTAGTTCTACTTTCTTTGACATGAATGTAGAACAAACTTTCATGGAACAAAGAGGAGAAGATCACCAGGAGATTAAAACCCTTTCACTGTTTCCCGTGCATGGTGAGGATATCTTTGACAACATGAAGACTACTTCCGATGAAGGTGGCGGTCACGGTGGTGGCTCTCACATTTCCCTTGAGCTCAGCCTTTCCTGCGAGGGCAAAGCTGGAAAAAATTTCCGGAGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

247

Amino Acids

27.4

Weight (kDa)

7.65

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 27 - 89 3.8e-10 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 394
Acc36I ACCTGC 1 cut(s) 394
AccIII TCCGGA 1 cut(s) 732
AciI CCGC 3 cut(s) 82, 402, 660
AclWI GGATC 4 cut(s) 188, 363, 382, 463
AcsI RAATTY 1 cut(s) 727
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 611
AgsI TTSAA 2 cut(s) 220, 492
AhlI ACTAGT 1 cut(s) 512
AjnI CCWGG 2 cut(s) 15, 576
AleI CACNNNNGTG 1 cut(s) 669
AluBI AGCT 6 cut(s) 130, 193, 500, 694, 719, 738
AluI AGCT 6 cut(s) 130, 193, 500, 694, 719, 738
Alw21I GWGCWC 1 cut(s) 696
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 4 cut(s) 188, 363, 382, 463
Ama87I CYCGRG 1 cut(s) 255
Aor13HI TCCGGA 1 cut(s) 732
ApeKI GCWGC 2 cut(s) 50, 738
ApoI RAATTY 1 cut(s) 727
Asp700I GAANNNNTTC 1 cut(s) 514
AspS9I GGNCC 1 cut(s) 153
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 3 cut(s) 335, 566, 626
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 1 cut(s) 153
BaeGI GKGCMC 1 cut(s) 300
BanII GRGCYC 2 cut(s) 256, 696
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 1 cut(s) 696
BbvI GCAGC 2 cut(s) 62, 725
BccI CCATC 2 cut(s) 26, 427
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 2 cut(s) 17, 578
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 191
BcuI ACTAGT 1 cut(s) 512
BfaI CTAG 2 cut(s) 480, 513
BfmI CTRYAG 1 cut(s) 102
BfuAI ACCTGC 1 cut(s) 394
BisI GCNGC 2 cut(s) 51, 739
BlpI GCTNAGC 1 cut(s) 695
BlsI GCNGC 2 cut(s) 52, 740
Bme1390I CCNGG 3 cut(s) 17, 55, 578
Bme18I GGWCC 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 255
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 3 cut(s) 6, 21, 155
BmrFI CCNGG 3 cut(s) 17, 55, 578
BpiI GAAGAC 1 cut(s) 644
BpmI CTGGAG 2 cut(s) 173, 522
Bpu1102I GCTNAGC 1 cut(s) 695
BpuEI CTTGAG 2 cut(s) 107, 710
BpuMI CCSGG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 15, 27
BsaWI WCCGGW 1 cut(s) 732
Bsc4I CCNNNNNNNGG 1 cut(s) 611
Bse8I GATNNNNATC 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 732
BseBI CCWGG 2 cut(s) 17, 578
BseDI CCNNGG 2 cut(s) 15, 27
BseGI GGATG 2 cut(s) 37, 292
BseJI GATNNNNATC 1 cut(s) 116
BseLI CCNNNNNNNGG 1 cut(s) 611
BseMII CTCAG 1 cut(s) 709
BseRI GAGGAG 1 cut(s) 579
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 2 cut(s) 62, 725
BsgI GTGCAG 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 696
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 2 cut(s) 54, 733
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 1 cut(s) 611
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 255
Bsp1286I GDGCHC 3 cut(s) 256, 300, 696
Bsp13I TCCGGA 1 cut(s) 732
Bsp143I GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
Bsp1720I GCTNAGC 1 cut(s) 695
BspACI CCGC 3 cut(s) 82, 402, 660
BspCNI CTCAG 1 cut(s) 708
BspEI TCCGGA 1 cut(s) 732
BspLI GGNNCC 3 cut(s) 6, 21, 155
BspMI ACCTGC 1 cut(s) 394
BspPI GGATC 4 cut(s) 188, 363, 382, 463
BssECI CCNNGG 2 cut(s) 15, 27
BssMI GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 2 cut(s) 17, 578
Bst4CI ACNGT 2 cut(s) 600, 668
Bst6I CTCTTC 1 cut(s) 467
BstDEI CTNAG 2 cut(s) 496, 695
BstF5I GGATG 2 cut(s) 37, 292
BstKTI GATC 8 cut(s) 109, 183, 213, 311, 358, 377, 458, 574
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
BstMWI GCNNNNNNNGC 3 cut(s) 79, 401, 705
BstNI CCWGG 2 cut(s) 17, 578
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 3 cut(s) 15, 53, 576
BstSFI CTRYAG 1 cut(s) 102
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 2 cut(s) 62, 725
BstV2I GAAGAC 1 cut(s) 644
BstX2I RGATCY 3 cut(s) 180, 374, 455
BstYI RGATCY 3 cut(s) 180, 374, 455
BtsCI GGATG 2 cut(s) 37, 292
BtsIMutI CAGTG 1 cut(s) 596
BveI ACCTGC 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 202
CviAII CATG 9 cut(s) 70, 293, 301, 350, 466, 532, 553, 611, 634
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 2 cut(s) 496, 695
DpnI GATC 8 cut(s) 108, 182, 212, 310, 357, 376, 457, 573
DpnII GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
Eam1104I CTCTTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 467
EciI GGCGGA 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 694
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 2 cut(s) 256, 696
Eco32I GATATC 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 153
Eco53kI GAGCTC 1 cut(s) 694
Eco88I CYCGRG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 694
EcoO109I RGGNCCY 1 cut(s) 153
EcoRII CCWGG 2 cut(s) 15, 576
EcoRV GATATC 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 2 cut(s) 256, 696
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 9 cut(s) 73, 296, 304, 353, 469, 535, 556, 614, 637
FaqI GGGAC 1 cut(s) 139
FatI CATG 9 cut(s) 69, 292, 300, 349, 465, 531, 552, 610, 633
Fnu4HI GCNGC 2 cut(s) 51, 739
FokI GGATG 2 cut(s) 44, 299
FriOI GRGCYC 2 cut(s) 256, 696
Fsp4HI GCNGC 2 cut(s) 51, 739
FspBI CTAG 2 cut(s) 480, 513
GluI GCNGC 2 cut(s) 51, 739
GsuI CTGGAG 2 cut(s) 173, 522
HapII CCGG 2 cut(s) 54, 733
Hin1II CATG 9 cut(s) 73, 296, 304, 353, 469, 535, 556, 614, 637
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 3 cut(s) 94, 117, 172
HpaI GTTAAC 1 cut(s) 424
HpaII CCGG 2 cut(s) 54, 733
HphI GGTGA 3 cut(s) 335, 566, 626
Hpy166II GTNNAC 3 cut(s) 244, 279, 424
Hpy188I TCNGA 4 cut(s) 111, 177, 286, 649
Hpy188III TCNNGA 4 cut(s) 220, 241, 257, 733
Hpy8I GTNNAC 3 cut(s) 244, 279, 424
HpyAV CCTTC 1 cut(s) 647
HpyCH4III ACNGT 2 cut(s) 600, 668
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 3 cut(s) 304, 383, 610
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 401, 705
HpyF3I CTNAG 2 cut(s) 496, 695
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 9 cut(s) 73, 296, 304, 353, 469, 535, 556, 614, 637
Kpn2I TCCGGA 1 cut(s) 732
KspAI GTTAAC 1 cut(s) 424
Kzo9I GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
LmnI GCTCC 2 cut(s) 127, 735
Lsp1109I GCAGC 2 cut(s) 62, 725
MaeI CTAG 2 cut(s) 480, 513
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 2 cut(s) 334, 662
MalI GATC 8 cut(s) 108, 182, 212, 310, 357, 376, 457, 573
MboI GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
MboII GAAGA 7 cut(s) 280, 283, 369, 390, 484, 581, 649
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 3 cut(s) 180, 374, 455
MhlI GDGCHC 3 cut(s) 256, 300, 696
MluCI AATT 2 cut(s) 340, 727
MnlI CCTC 8 cut(s) 29, 131, 171, 346, 497, 557, 610, 703
MroI TCCGGA 1 cut(s) 732
MroXI GAANNNNTTC 1 cut(s) 514
MseI TTAA 3 cut(s) 366, 423, 585
MslI CAYNNNNRTG 1 cut(s) 669
MspA1I CMGCKG 2 cut(s) 193, 404
MspI CCGG 2 cut(s) 54, 733
MspR9I CCNGG 3 cut(s) 17, 55, 578
MunI CAATTG 1 cut(s) 340
MvaI CCWGG 2 cut(s) 17, 578
MwoI GCNNNNNNNGC 3 cut(s) 79, 401, 705
NciI CCSGG 1 cut(s) 55
NdeII GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
NlaIII CATG 9 cut(s) 73, 296, 304, 353, 469, 535, 556, 614, 637
NlaIV GGNNCC 3 cut(s) 6, 21, 155
NmuCI GTSAC 1 cut(s) 662
NspI RCATGY 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 669
PaqCI CACCTGC 1 cut(s) 394
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 514
PfeI GAWTC 3 cut(s) 94, 117, 172
PkrI GCNGC 2 cut(s) 52, 740
PpuMI RGGWCCY 1 cut(s) 153
Psp124BI GAGCTC 1 cut(s) 696
Psp5II RGGWCCY 1 cut(s) 153
Psp6I CCWGG 2 cut(s) 15, 576
PspGI CCWGG 2 cut(s) 15, 576
PspN4I GGNNCC 3 cut(s) 6, 21, 155
PspPI GGNCC 1 cut(s) 153
PspPPI RGGWCCY 1 cut(s) 153
PsrI GAACNNNNNNTAC 2 cut(s) 502, 534
PsuI RGATCY 3 cut(s) 180, 374, 455
PvuII CAGCTG 1 cut(s) 193
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
RseI CAYNNNNRTG 1 cut(s) 669
SacI GAGCTC 1 cut(s) 696
SaqAI TTAA 3 cut(s) 366, 423, 585
SatI GCNGC 2 cut(s) 51, 739
Sau3AI GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
Sau96I GGNCC 1 cut(s) 153
ScrFI CCNGG 3 cut(s) 17, 55, 578
SduI GDGCHC 3 cut(s) 256, 300, 696
SfcI CTRYAG 1 cut(s) 102
SinI GGWCC 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 669
SmlI CTYRAG 2 cut(s) 122, 689
SmoI CTYRAG 2 cut(s) 122, 689
SpeI ACTAGT 1 cut(s) 512
Sse9I AATT 2 cut(s) 340, 727
SsiI CCGC 3 cut(s) 82, 402, 660
SspMI CTAG 2 cut(s) 480, 513
SstI GAGCTC 1 cut(s) 696
StyD4I CCNGG 3 cut(s) 15, 53, 576
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 2 cut(s) 600, 668
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 213
TasI AATT 2 cut(s) 340, 727
TfiI GAWTC 3 cut(s) 94, 117, 172
Tru1I TTAA 3 cut(s) 366, 423, 585
Tru9I TTAA 3 cut(s) 366, 423, 585
TscAI CASTG 1 cut(s) 603
TseFI GTSAC 1 cut(s) 662
TseI GCWGC 2 cut(s) 50, 738
Tsp45I GTSAC 1 cut(s) 662
TspDTI ATGAA 6 cut(s) 281, 476, 541, 548, 650, 666
TspRI CASTG 1 cut(s) 603
VpaK11BI GGWCC 1 cut(s) 153
XapI RAATTY 1 cut(s) 727
XceI RCATGY 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 514
XspI CTAG 2 cut(s) 480, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.