MD07G1108600.v1.1

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007126
Physical Location & Seq
Forward (+)
12470578 .. 12471838
1261 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1108600.v1.1.491

Sequence Viewer

Length: 786 bp
ATGTACATGTGGAGATCAATGCACCAGTACCATGAAGTCCAGAACGACATTGTACAACAGGTCAGGGGACTTGTGAACCGATCAGCGAAGGGTGACTCAACTTCGGAACTACATCGGCAGGCAACACGTGACCTTGAATCAGCTGTCTCTGCCTGGCACTCCAGTTTCTGCCGTTTAATAAAGTTCAAACGGGACTTTATCCGGTCTGTTCATGGTTGGTTCAAGCTCACCCTTCTTCCTGTAAACAACGACATGACTTTCAATGTGCACAATGAATCTTCTGATGTATATTCCTTCTGTGATGAGTGGAAGCTTGCACTTGAGCGCGTCCCTGACACAGTAGCTTCTGAGGCTATCAATAGCTTTATCAATGTTGTCCACGTAATCTCTGTGAAACAGAGTGAAGAGCTCAAGATCAAAAAACGCACTGAAACTGCGTCAAAGGAGCTTGAGAAAAAGGCTTCTTCTCTCCGAAACATTGAAAAGAAATTCTACCACTCCTACTCCATGGTTGGTATTGGGCTTCCTGACTCGGGGCCTGAAAATGGGCAGGTTTTGGATGCGCGAGATCCTCTTGCTGAGAAGAAATCTGAACTTACAACCTGCCAGAGGCGGGTTGAAGATGAGATGATGAGGCATACAAAGGCGGTGGAGGTGACAAGAGCCATGACTCTAAACAATCTTCAAACAGGCTTGCCAGGAGTATTTCAGGCACTGACCAGCTTTGCTGGCCTATTTACGGAGGCACTTGAGTCGGTTTGTACACGTTCCTACGCCATCAAATAG

Protein Analysis

262

Amino Acids

29.81

Weight (kDa)

8.28

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 1 - 127 5.5e-38 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 541, 611
AccII CGCG 2 cut(s) 327, 565
AciI CCGC 2 cut(s) 613, 647
AclWI GGATC 1 cut(s) 563
AcsI RAATTY 1 cut(s) 488
AcvI CACGTG 1 cut(s) 128
AfaI GTAC 4 cut(s) 5, 29, 54, 763
AfiI CCNNNNNNNGG 5 cut(s) 533, 545, 609, 613, 739
AflIII ACRYGT 3 cut(s) 6, 125, 764
AgsI TTSAA 7 cut(s) 137, 187, 223, 262, 482, 620, 686
AjnI CCWGG 2 cut(s) 152, 697
AluBI AGCT 8 cut(s) 143, 226, 313, 344, 363, 409, 448, 723
AluI AGCT 8 cut(s) 143, 226, 313, 344, 363, 409, 448, 723
Alw21I GWGCWC 2 cut(s) 270, 411
Alw26I GTCTC 1 cut(s) 151
Alw44I GTGCAC 1 cut(s) 266
AlwI GGATC 1 cut(s) 563
AlwNI CAGNNNCTG 2 cut(s) 168, 715
Ama87I CYCGRG 1 cut(s) 532
AoxI GGCC 2 cut(s) 536, 730
ApaLI GTGCAC 1 cut(s) 266
ApoI RAATTY 1 cut(s) 488
AspLEI GCGC 2 cut(s) 327, 565
AspS9I GGNCC 1 cut(s) 536
AsuHPI GGTGA 3 cut(s) 104, 220, 667
AvaI CYCGRG 1 cut(s) 532
BaeGI GKGCMC 1 cut(s) 270
BanII GRGCYC 1 cut(s) 411
BbrPI CACGTG 1 cut(s) 128
Bbv12I GWGCWC 2 cut(s) 270, 411
BccI CCATC 1 cut(s) 785
BceAI ACGGC 1 cut(s) 156
BcgI CGANNNNNNTGC 2 cut(s) 735, 769
BciT130I CCWGG 2 cut(s) 154, 699
BcoDI GTCTC 1 cut(s) 151
BfuAI ACCTGC 2 cut(s) 541, 611
Bme1390I CCNGG 2 cut(s) 154, 699
BmeT110I CYCGRG 1 cut(s) 532
BmgT120I GGNCC 1 cut(s) 536
BmiI GGNNCC 1 cut(s) 537
BmrFI CCNGG 2 cut(s) 154, 699
BmsI GCATC 1 cut(s) 550
BpmI CTGGAG 1 cut(s) 145
BpuEI CTTGAG 4 cut(s) 341, 395, 470, 770
BsaAI YACGTR 2 cut(s) 128, 382
BsaJI CCNNGG 1 cut(s) 507
BsaWI WCCGGW 1 cut(s) 201
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 5 cut(s) 533, 545, 609, 613, 739
Bse1I ACTGG 2 cut(s) 25, 162
BseBI CCWGG 2 cut(s) 154, 699
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 1 cut(s) 565
BseLI CCNNNNNNNGG 5 cut(s) 533, 545, 609, 613, 739
BseMII CTCAG 2 cut(s) 339, 570
BseNI ACTGG 2 cut(s) 25, 162
BseSI GKGCMC 1 cut(s) 270
Bsh1236I CGCG 2 cut(s) 327, 565
BshFI GGCC 2 cut(s) 538, 732
BsiHKAI GWGCWC 2 cut(s) 270, 411
BsiHKCI CYCGRG 1 cut(s) 532
BsiSI CCGG 1 cut(s) 202
BslFI GGGAC 3 cut(s) 81, 206, 314
BslI CCNNNNNNNGG 5 cut(s) 533, 545, 609, 613, 739
BsmAI GTCTC 1 cut(s) 151
BsmFI GGGAC 3 cut(s) 81, 206, 314
BsnI GGCC 2 cut(s) 538, 732
BsoBI CYCGRG 1 cut(s) 532
Bsp1286I GDGCHC 2 cut(s) 270, 411
Bsp1407I TGTACA 3 cut(s) 3, 52, 761
Bsp143I GATC 4 cut(s) 14, 80, 414, 568
Bsp19I CCATGG 1 cut(s) 507
BspACI CCGC 2 cut(s) 613, 647
BspANI GGCC 2 cut(s) 538, 732
BspCNI CTCAG 2 cut(s) 340, 571
BspFNI CGCG 2 cut(s) 327, 565
BspLI GGNNCC 1 cut(s) 537
BspMI ACCTGC 2 cut(s) 541, 611
BspPI GGATC 1 cut(s) 563
BspQI GCTCTTC 1 cut(s) 399
BsrGI TGTACA 3 cut(s) 3, 52, 761
BsrI ACTGG 2 cut(s) 25, 162
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 4 cut(s) 14, 80, 414, 568
BssT1I CCWWGG 1 cut(s) 507
Bst2UI CCWGG 2 cut(s) 154, 699
Bst4CI ACNGT 1 cut(s) 340
Bst6I CTCTTC 1 cut(s) 399
BstAUI TGTACA 3 cut(s) 3, 52, 761
BstBAI YACGTR 2 cut(s) 128, 382
BstC8I GCNNGC 4 cut(s) 120, 315, 695, 730
BstDEI CTNAG 2 cut(s) 348, 579
BstDSI CCRYGG 1 cut(s) 507
BstENI CCTNNNNNAGG 1 cut(s) 607
BstF5I GGATG 1 cut(s) 565
BstFNI CGCG 2 cut(s) 327, 565
BstHHI GCGC 2 cut(s) 327, 565
BstKTI GATC 4 cut(s) 17, 83, 417, 571
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 4 cut(s) 14, 80, 414, 568
BstMWI GCNNNNNNNGC 3 cut(s) 149, 350, 729
BstNI CCWGG 2 cut(s) 154, 699
BstNSI RCATGY 1 cut(s) 10
BstSCI CCNGG 2 cut(s) 152, 697
BstSLI GKGCMC 1 cut(s) 270
BstUI CGCG 2 cut(s) 327, 565
BstX2I RGATCY 1 cut(s) 568
BstYI RGATCY 1 cut(s) 568
BsuRI GGCC 2 cut(s) 538, 732
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 1 cut(s) 565
BtsIMutI CAGTG 2 cut(s) 426, 713
BveI ACCTGC 2 cut(s) 541, 611
Cac8I GCNNGC 4 cut(s) 120, 315, 695, 730
CaiI CAGNNNCTG 2 cut(s) 168, 715
CfoI GCGC 2 cut(s) 327, 565
Cfr13I GGNCC 1 cut(s) 536
CseI GACGC 2 cut(s) 316, 426
Csp6I GTAC 4 cut(s) 4, 28, 53, 762
CspCI CAANNNNNGTGG 2 cut(s) 630, 665
CviAII CATG 6 cut(s) 7, 32, 212, 253, 508, 667
CviQI GTAC 4 cut(s) 4, 28, 53, 762
DdeI CTNAG 2 cut(s) 348, 579
DpnI GATC 4 cut(s) 16, 82, 416, 570
DpnII GATC 4 cut(s) 14, 80, 414, 568
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Ecl136II GAGCTC 1 cut(s) 409
Eco130I CCWWGG 1 cut(s) 507
Eco24I GRGCYC 1 cut(s) 411
Eco53kI GAGCTC 1 cut(s) 409
Eco72I CACGTG 1 cut(s) 128
Eco88I CYCGRG 1 cut(s) 532
EcoICRI GAGCTC 1 cut(s) 409
EcoNI CCTNNNNNAGG 1 cut(s) 607
EcoO109I RGGNCCY 1 cut(s) 536
EcoRII CCWGG 2 cut(s) 152, 697
EcoT14I CCWWGG 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 411
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 6 cut(s) 10, 35, 215, 256, 511, 670
FaiI YATR 8 cut(s) 8, 33, 213, 254, 289, 509, 639, 668
FaqI GGGAC 3 cut(s) 81, 206, 314
FatI CATG 6 cut(s) 6, 31, 211, 252, 507, 666
FauI CCCGC 1 cut(s) 606
FokI GGATG 1 cut(s) 572
FriOI GRGCYC 1 cut(s) 411
GlaI GCGC 2 cut(s) 326, 564
GsuI CTGGAG 1 cut(s) 145
HaeIII GGCC 2 cut(s) 538, 732
HapII CCGG 1 cut(s) 202
HgaI GACGC 2 cut(s) 316, 426
HhaI GCGC 2 cut(s) 327, 565
Hin1II CATG 6 cut(s) 10, 35, 215, 256, 511, 670
Hin6I GCGC 2 cut(s) 325, 563
HinP1I GCGC 2 cut(s) 325, 563
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 6 cut(s) 95, 137, 275, 530, 670, 752
HpaII CCGG 1 cut(s) 202
HphI GGTGA 3 cut(s) 104, 220, 667
Hpy166II GTNNAC 5 cut(s) 76, 244, 268, 379, 764
Hpy188I TCNGA 5 cut(s) 106, 283, 349, 473, 592
Hpy188III TCNNGA 3 cut(s) 40, 412, 527
Hpy8I GTNNAC 5 cut(s) 76, 244, 268, 379, 764
HpyAV CCTTC 3 cut(s) 82, 242, 304
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4IV ACGT 3 cut(s) 127, 381, 766
HpyCH4V TGCA 3 cut(s) 22, 268, 317
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 350, 729
HpyF3I CTNAG 2 cut(s) 348, 579
HpySE526I ACGT 3 cut(s) 127, 381, 766
Hsp92II CATG 6 cut(s) 10, 35, 215, 256, 511, 670
HspAI GCGC 2 cut(s) 325, 563
Kzo9I GATC 4 cut(s) 14, 80, 414, 568
LguI GCTCTTC 1 cut(s) 399
LmnI GCTCC 1 cut(s) 445
LweI GCATC 1 cut(s) 550
MaeII ACGT 3 cut(s) 127, 381, 766
MaeIII GTNAC 3 cut(s) 92, 128, 655
MalI GATC 4 cut(s) 16, 82, 416, 570
MboI GATC 4 cut(s) 14, 80, 414, 568
MboII GAAGA 7 cut(s) 227, 270, 416, 456, 595, 632, 674
MflI RGATCY 1 cut(s) 568
MhlI GDGCHC 2 cut(s) 270, 411
MluCI AATT 1 cut(s) 488
MlyI GAGTC 4 cut(s) 89, 524, 664, 761
MnlI CCTC 6 cut(s) 343, 582, 603, 627, 646, 736
MseI TTAA 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 2 cut(s) 154, 699
MvaI CCWGG 2 cut(s) 154, 699
MvnI CGCG 2 cut(s) 327, 565
MwoI GCNNNNNNNGC 3 cut(s) 149, 350, 729
NcoI CCATGG 1 cut(s) 507
NdeII GATC 4 cut(s) 14, 80, 414, 568
NlaIII CATG 6 cut(s) 10, 35, 215, 256, 511, 670
NlaIV GGNNCC 1 cut(s) 537
NmuCI GTSAC 3 cut(s) 92, 128, 655
NspI RCATGY 1 cut(s) 10
PciI ACATGT 1 cut(s) 6
PciSI GCTCTTC 1 cut(s) 399
PfeI GAWTC 2 cut(s) 137, 275
PleI GAGTC 4 cut(s) 89, 524, 664, 760
PmaCI CACGTG 1 cut(s) 128
PmlI CACGTG 1 cut(s) 128
PpsI GAGTC 4 cut(s) 89, 524, 664, 760
Ppu21I YACGTR 2 cut(s) 128, 382
PscI ACATGT 1 cut(s) 6
Psp124BI GAGCTC 1 cut(s) 411
Psp6I CCWGG 2 cut(s) 152, 697
PspCI CACGTG 1 cut(s) 128
PspGI CCWGG 2 cut(s) 152, 697
PspN4I GGNNCC 1 cut(s) 537
PspPI GGNCC 1 cut(s) 536
PstNI CAGNNNCTG 2 cut(s) 168, 715
PsuI RGATCY 1 cut(s) 568
PvuII CAGCTG 1 cut(s) 143
RsaI GTAC 4 cut(s) 5, 29, 54, 763
RsaNI GTAC 4 cut(s) 4, 28, 53, 762
SacI GAGCTC 1 cut(s) 411
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 1 cut(s) 176
Sau3AI GATC 4 cut(s) 14, 80, 414, 568
Sau96I GGNCC 1 cut(s) 536
SchI GAGTC 4 cut(s) 89, 524, 664, 761
ScrFI CCNGG 2 cut(s) 154, 699
SduI GDGCHC 2 cut(s) 270, 411
SfaNI GCATC 1 cut(s) 550
SmlI CTYRAG 4 cut(s) 320, 410, 449, 749
SmoI CTYRAG 4 cut(s) 320, 410, 449, 749
Sse9I AATT 1 cut(s) 488
SsiI CCGC 2 cut(s) 613, 647
SstI GAGCTC 1 cut(s) 411
StyD4I CCNGG 2 cut(s) 152, 697
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 1 cut(s) 340
TaiI ACGT 3 cut(s) 130, 384, 769
TasI AATT 1 cut(s) 488
TatI WGTACW 3 cut(s) 3, 52, 761
TfiI GAWTC 2 cut(s) 137, 275
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TscAI CASTG 2 cut(s) 433, 720
TseFI GTSAC 3 cut(s) 92, 128, 655
Tsp45I GTSAC 3 cut(s) 92, 128, 655
TspDTI ATGAA 3 cut(s) 48, 200, 288
TspGWI ACGGA 1 cut(s) 755
TspRI CASTG 2 cut(s) 433, 720
VneI GTGCAC 1 cut(s) 266
XagI CCTNNNNNAGG 1 cut(s) 607
XapI RAATTY 1 cut(s) 488
XceI RCATGY 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.