MD07G1148600.v1.1

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0016391
Physical Location & Seq
Reverse (-)
21717908 .. 21718249
342 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1148600.v1.1.491

Sequence Viewer

Length: 342 bp
ATGTCTCGCCTAATCGGATTCTTAGCCGTTCTCCTGCTCCTTTCGATCACTGCACACTCGGCGCCTTCATGCCCTACGGTGAAACAGGAGGTGGCCCCGTGCGTCACTTTCGTGAAAGGTGGAGCCGACGCAAAGCCATCGGGGGAGTGTTGCAAAGGTGTCAAGAACCTGAGCGTGAATGCCGCTAACAAGGCAGACAAGGAAGCAGTTTGCTTGTGCCTCAAGCAAGCGTTGTCTACCATTGGAACCTATAATCCCTCTCAAATCTCTGCGCTTCCCAAGAAGTGTGGCATCTCCCTCGACCTCCCTGCAATTGACAAAGACACCGACTGCACAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

114

Amino Acids

11.71

Weight (kDa)

8.65

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 17 - 100 1.4e-09 Probable lipid transfer
Tryp_alpha_amyl PF00234 24 - 111 2.2e-12 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016391)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g16320
malus_domestica MD01G1079700.v1.1 MD07G1148600.v1.1
prunus_persica Prupe.2G187100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0359331
rosa_laevigata RLG00000027879
rosa_multiflora Rmu_sc0013596.1_g000002 Rmu_sc0017222.1_g000002
rosa_roxburghii Rroxscaffold_4G00297090
rosa_rugosa Rorug01G0270100
rosa_samantha Rh1AG283600 Rh1BG249800 Rh1CG267700 Rh1DG278700
rosa_wichuraiana Rw1G025180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 61
AccI GTMKAC 1 cut(s) 236
AciI CCGC 1 cut(s) 183
AcyI GRCGYC 1 cut(s) 62
AleI CACNNNNGTG 1 cut(s) 110
AloI GAACNNNNNNTCC 2 cut(s) 238, 270
Alw26I GTCTC 1 cut(s) 9
AoxI GGCC 1 cut(s) 93
AspLEI GCGC 2 cut(s) 64, 274
AspS9I GGNCC 1 cut(s) 94
AsuHPI GGTGA 1 cut(s) 91
BanI GGYRCC 1 cut(s) 61
BccI CCATC 1 cut(s) 145
BceAI ACGGC 1 cut(s) 11
BcgI CGANNNNNNTGC 6 cut(s) 120, 154, 280, 290, 314, 324
BcoDI GTCTC 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 65
BisI GCNGC 1 cut(s) 183
BlsI GCNGC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 4 cut(s) 63, 96, 124, 247
BmsI GCATC 1 cut(s) 300
Bpu10I CCTNAGC 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 62
BseMII CTCAG 1 cut(s) 161
BsgI GTGCAG 2 cut(s) 36, 316
BshFI GGCC 1 cut(s) 95
BshNI GGYRCC 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 9
BsmI GAATGC 1 cut(s) 184
BsnI GGCC 1 cut(s) 95
Bsp143I GATC 1 cut(s) 45
BspACI CCGC 1 cut(s) 183
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 1 cut(s) 162
BspLI GGNNCC 4 cut(s) 63, 96, 124, 247
BspT107I GGYRCC 1 cut(s) 61
BssMI GATC 1 cut(s) 45
BssNI GRCGYC 1 cut(s) 62
Bst4CI ACNGT 1 cut(s) 79
BstACI GRCGYC 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 2 cut(s) 22, 170
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 2 cut(s) 64, 274
BstKTI GATC 1 cut(s) 48
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 1 cut(s) 45
BstMWI GCNNNNNNNGC 2 cut(s) 59, 191
BsuRI GGCC 1 cut(s) 95
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 228
CfoI GCGC 2 cut(s) 64, 274
Cfr13I GGNCC 1 cut(s) 94
CseI GACGC 2 cut(s) 91, 137
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 1 cut(s) 69
CviJI RGCY 4 cut(s) 26, 95, 125, 136
CviKI_1 RGCY 4 cut(s) 26, 95, 125, 136
DdeI CTNAG 2 cut(s) 22, 170
DinI GGCGCC 1 cut(s) 63
DpnI GATC 1 cut(s) 47
DpnII GATC 1 cut(s) 45
EgeI GGCGCC 1 cut(s) 63
EheI GGCGCC 1 cut(s) 63
FaeI CATG 1 cut(s) 72
FaiI YATR 2 cut(s) 70, 252
FatI CATG 1 cut(s) 68
FblI GTMKAC 1 cut(s) 236
Fnu4HI GCNGC 1 cut(s) 183
Fsp4HI GCNGC 1 cut(s) 183
GlaI GCGC 2 cut(s) 63, 273
GluI GCNGC 1 cut(s) 183
HaeII RGCGCY 1 cut(s) 65
HaeIII GGCC 1 cut(s) 95
HgaI GACGC 2 cut(s) 91, 137
HhaI GCGC 2 cut(s) 64, 274
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 1 cut(s) 72
Hin6I GCGC 2 cut(s) 62, 272
HinP1I GCGC 2 cut(s) 62, 272
HinfI GANTC 1 cut(s) 18
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 1 cut(s) 237
Hpy188I TCNGA 1 cut(s) 17
Hpy188III TCNNGA 2 cut(s) 112, 163
Hpy8I GTNNAC 1 cut(s) 237
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4V TGCA 4 cut(s) 53, 153, 311, 333
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 191
HpyF3I CTNAG 2 cut(s) 22, 170
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 1 cut(s) 72
HspAI GCGC 2 cut(s) 62, 272
KasI GGCGCC 1 cut(s) 61
Kzo9I GATC 1 cut(s) 45
LmnI GCTCC 2 cut(s) 42, 122
LpnPI CCDG 4 cut(s) 47, 71, 182, 321
LweI GCATC 1 cut(s) 300
MaeIII GTNAC 1 cut(s) 103
MalI GATC 1 cut(s) 47
MboI GATC 1 cut(s) 45
MfeI CAATTG 1 cut(s) 312
MluCI AATT 1 cut(s) 312
Mly113I GGCGCC 1 cut(s) 62
MnlI CCTC 5 cut(s) 82, 230, 268, 308, 314
MslI CAYNNNNRTG 1 cut(s) 110
MunI CAATTG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 184
MwoI GCNNNNNNNGC 2 cut(s) 59, 191
NarI GGCGCC 1 cut(s) 62
NdeII GATC 1 cut(s) 45
NlaIII CATG 1 cut(s) 72
NlaIV GGNNCC 4 cut(s) 63, 96, 124, 247
NmeAIII GCCGAG 1 cut(s) 38
NmuCI GTSAC 1 cut(s) 103
OliI CACNNNNGTG 1 cut(s) 110
PctI GAATGC 1 cut(s) 184
PfeI GAWTC 1 cut(s) 18
PkrI GCNGC 1 cut(s) 184
PluTI GGCGCC 1 cut(s) 65
PspN4I GGNNCC 4 cut(s) 63, 96, 124, 247
PspPI GGNCC 1 cut(s) 94
RseI CAYNNNNRTG 1 cut(s) 110
SatI GCNGC 1 cut(s) 183
Sau3AI GATC 1 cut(s) 45
Sau96I GGNCC 1 cut(s) 94
SetI ASST 6 cut(s) 93, 121, 160, 171, 251, 306
SfaNI GCATC 1 cut(s) 300
SfoI GGCGCC 1 cut(s) 63
SmiMI CAYNNNNRTG 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 1 cut(s) 312
SsiI CCGC 1 cut(s) 183
SspDI GGCGCC 1 cut(s) 61
TaaI ACNGT 1 cut(s) 79
TaqI TCGA 2 cut(s) 44, 300
TasI AATT 1 cut(s) 312
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 1 cut(s) 18
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 1 cut(s) 103
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 1 cut(s) 57
TspRI CASTG 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.