Rroxscaffold_4G00297090

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016391
Physical Location & Seq
Forward (+)
17077849 .. 17078326
478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00297090.1

Sequence Viewer

Length: 354 bp
ATGGCTCGCTTGATTGCATTCTTAGCCATTCTCTTCTTCGTTTCGGCTGCACATTCTGCGCCTTCATGCCCCGTCATAGCCCAGGAGGTGTCCCCTTGTCTCGCTTTTGTGAAGGGTACAACTGAAGAGCTATCTCAGGCTTGTTGCAATGGTGCTAAGGACCTGAGCAACAGTGTCCATTCCAATCAAGACAAGAAAGACATATGCCTGTGCCTCAAGGGCGCCTTGTCCAAGATTGGAGACTATGAGCCATCTAGACTCTCTCTTGTTCCCGAGAGATGTGGCCTGAAGCTCGACCTTCCTCCAATCGATCAAAACACCGACTGCTCCAAGTTTGCTCTAATGGGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

117

Amino Acids

12.55

Weight (kDa)

6.05

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 14 - 98 2.6e-06 Probable lipid transfer
Tryp_alpha_amyl PF00234 23 - 109 3.3e-09 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016391)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g16320
malus_domestica MD01G1079700.v1.1 MD07G1148600.v1.1
prunus_persica Prupe.2G187100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0359331
rosa_laevigata RLG00000027879
rosa_multiflora Rmu_sc0013596.1_g000002 Rmu_sc0017222.1_g000002
rosa_roxburghii Rroxscaffold_4G00297090
rosa_rugosa Rorug01G0270100
rosa_samantha Rh1AG283600 Rh1BG249800 Rh1CG267700 Rh1DG278700
rosa_wichuraiana Rw1G025180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 221
AcuI CTGAAG 2 cut(s) 144, 308
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 1 cut(s) 118
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 2 cut(s) 130, 292
AluI AGCT 2 cut(s) 130, 292
Alw26I GTCTC 2 cut(s) 104, 234
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 1 cut(s) 283
ApeKI GCWGC 1 cut(s) 47
AspLEI GCGC 2 cut(s) 61, 224
AspS9I GGNCC 1 cut(s) 160
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 1 cut(s) 160
BanI GGYRCC 1 cut(s) 221
BbvI GCAGC 1 cut(s) 34
BccI CCATC 1 cut(s) 259
BciT130I CCWGG 1 cut(s) 83
BcoDI GTCTC 2 cut(s) 104, 234
BfaI CTAG 1 cut(s) 255
BfoI RGCGCY 1 cut(s) 225
BglI GCCNNNNNGGC 1 cut(s) 219
BisI GCNGC 1 cut(s) 48
BlsI GCNGC 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 1 cut(s) 223
BmrFI CCNGG 1 cut(s) 83
Bpu10I CCTNAGC 2 cut(s) 156, 164
BpuEI CTTGAG 1 cut(s) 200
Bsa29I ATCGAT 1 cut(s) 309
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 81
Bse3DI GCAATG 1 cut(s) 154
BseBI CCWGG 1 cut(s) 83
BseCI ATCGAT 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 81
BseMI GCAATG 1 cut(s) 154
BseMII CTCAG 2 cut(s) 149, 155
BseXI GCAGC 1 cut(s) 34
BsgI GTGCAG 1 cut(s) 33
BshFI GGCC 1 cut(s) 285
BshNI GGYRCC 1 cut(s) 221
BshVI ATCGAT 1 cut(s) 309
BsiHKCI CYCGRG 1 cut(s) 272
BslFI GGGAC 1 cut(s) 76
BsmAI GTCTC 2 cut(s) 104, 234
BsmFI GGGAC 1 cut(s) 76
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 1 cut(s) 285
BsoBI CYCGRG 1 cut(s) 272
Bsp143I GATC 1 cut(s) 310
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 2 cut(s) 148, 156
BspDI ATCGAT 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 223
BspQI GCTCTTC 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 1 cut(s) 310
BssNI GRCGYC 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 173
Bst6I CTCTTC 2 cut(s) 38, 120
BstACI GRCGYC 1 cut(s) 222
BstAPI GCANNNNNTGC 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 4 cut(s) 22, 135, 156, 164
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 2 cut(s) 61, 224
BstKTI GATC 1 cut(s) 313
BstMAI GTCTC 2 cut(s) 104, 234
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 3 cut(s) 23, 56, 219
BstNI CCWGG 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 81
BstV1I GCAGC 1 cut(s) 34
Bsu15I ATCGAT 1 cut(s) 309
BsuRI GGCC 1 cut(s) 285
BsuTUI ATCGAT 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 178
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 2 cut(s) 61, 224
Cfr13I GGNCC 1 cut(s) 160
ClaI ATCGAT 1 cut(s) 309
Csp6I GTAC 1 cut(s) 117
CviAII CATG 1 cut(s) 66
CviJI RGCY 9 cut(s) 5, 26, 47, 80, 130, 140, 250, 285, 292
CviKI_1 RGCY 9 cut(s) 5, 26, 47, 80, 130, 140, 250, 285, 292
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 4 cut(s) 22, 135, 156, 164
DinI GGCGCC 1 cut(s) 223
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
Eam1104I CTCTTC 2 cut(s) 38, 120
EarI CTCTTC 2 cut(s) 38, 120
Eco47I GGWCC 1 cut(s) 160
Eco57I CTGAAG 2 cut(s) 144, 308
Eco88I CYCGRG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 81
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
FaeI CATG 1 cut(s) 69
FaiI YATR 5 cut(s) 67, 77, 203, 205, 246
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FaqI GGGAC 1 cut(s) 76
FatI CATG 1 cut(s) 65
FauNDI CATATG 1 cut(s) 203
Fnu4HI GCNGC 1 cut(s) 48
Fsp4HI GCNGC 1 cut(s) 48
FspBI CTAG 1 cut(s) 255
GlaI GCGC 2 cut(s) 60, 223
GluI GCNGC 1 cut(s) 48
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 1 cut(s) 285
HhaI GCGC 2 cut(s) 61, 224
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 1 cut(s) 69
Hin6I GCGC 2 cut(s) 59, 222
HinP1I GCGC 2 cut(s) 59, 222
HinfI GANTC 1 cut(s) 258
Hpy188III TCNNGA 3 cut(s) 188, 255, 272
HpyAV CCTTC 3 cut(s) 72, 106, 308
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4V TGCA 3 cut(s) 17, 50, 147
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 56, 219
HpyF3I CTNAG 4 cut(s) 22, 135, 156, 164
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 1 cut(s) 69
HspAI GCGC 2 cut(s) 59, 222
KasI GGCGCC 1 cut(s) 221
Kzo9I GATC 1 cut(s) 310
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 1 cut(s) 332
LpnPI CCDG 6 cut(s) 68, 95, 122, 176, 221, 299
Lsp1109I GCAGC 1 cut(s) 34
MaeI CTAG 1 cut(s) 255
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 3 cut(s) 25, 28, 137
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 1 cut(s) 252
MnlI CCTC 3 cut(s) 79, 224, 312
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 1 cut(s) 83
MwoI GCNNNNNNNGC 3 cut(s) 23, 56, 219
NarI GGCGCC 1 cut(s) 222
NdeI CATATG 1 cut(s) 203
NdeII GATC 1 cut(s) 310
NlaIII CATG 1 cut(s) 69
NlaIV GGNNCC 1 cut(s) 223
PciSI GCTCTTC 1 cut(s) 120
PctI GAATGC 1 cut(s) 17
PkrI GCNGC 1 cut(s) 49
PleI GAGTC 1 cut(s) 252
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 252
PpuMI RGGWCCY 1 cut(s) 160
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 1 cut(s) 223
PspPI GGNCC 1 cut(s) 160
PspPPI RGGWCCY 1 cut(s) 160
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
SapI GCTCTTC 1 cut(s) 120
SatI GCNGC 1 cut(s) 48
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 1 cut(s) 160
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 1 cut(s) 83
SetI ASST 5 cut(s) 90, 132, 165, 294, 300
SfoI GGCGCC 1 cut(s) 223
SinI GGWCC 1 cut(s) 160
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SspDI GGCGCC 1 cut(s) 221
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 173
TaqI TCGA 2 cut(s) 294, 309
TscAI CASTG 1 cut(s) 178
TseI GCWGC 1 cut(s) 47
TspDTI ATGAA 1 cut(s) 54
TspRI CASTG 1 cut(s) 178
VpaK11BI GGWCC 1 cut(s) 160
XbaI TCTAGA 1 cut(s) 254
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.