Rh1DG278700

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0016391
Physical Location & Seq
Reverse (-)
50048586 .. 50049473
888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG278700.1

Sequence Viewer

Length: 354 bp
ATGGCTCGCTTGATTGCATTCTTAGCCATTCTCTTCTTCGTTTCAGCTGCACATTCTGCTCCTTCATGCCCCGTCATAGCCCAGGAGCTGGCCCCTTGTCTCACTTTTGTGAAGGGTACAACTGAAGAGCTATCTCAGGCTTGTTGCAATGGTGCTAAGGACCTGAGCAACAATGTCCATTCCAACGAAGACAAGAAAGACATATGCCTGTGCCTCAAGGGCGCCTTGTCCAAGATTGGAGACTATGAGCCATCTAGAGTCTCTCTTGTTCCCAAGAAATGTGGCCTGAAGCTCGACCTTCCTCCAATCGATCAAAACACCGACTGCTCCAAGTTTGCTCTAATGGGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

117

Amino Acids

12.56

Weight (kDa)

6.69

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 14 - 98 1.4e-06 Probable lipid transfer
Tryp_alpha_amyl PF00234 23 - 109 2.2e-10 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016391)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g16320
malus_domestica MD01G1079700.v1.1 MD07G1148600.v1.1
prunus_persica Prupe.2G187100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0359331
rosa_laevigata RLG00000027879
rosa_multiflora Rmu_sc0013596.1_g000002 Rmu_sc0017222.1_g000002
rosa_roxburghii Rroxscaffold_4G00297090
rosa_rugosa Rorug01G0270100
rosa_samantha Rh1AG283600 Rh1BG249800 Rh1CG267700 Rh1DG278700
rosa_wichuraiana Rw1G025180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 221
AccB7I CCANNNNNTGG 1 cut(s) 88
AcuI CTGAAG 2 cut(s) 144, 308
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 1 cut(s) 118
AfiI CCNNNNNNNGG 1 cut(s) 88
AjnI CCWGG 1 cut(s) 81
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 4 cut(s) 47, 88, 130, 292
AluI AGCT 4 cut(s) 47, 88, 130, 292
Alw26I GTCTC 3 cut(s) 104, 234, 265
AlwNI CAGNNNCTG 1 cut(s) 88
AoxI GGCC 2 cut(s) 90, 283
ApeKI GCWGC 1 cut(s) 47
AspLEI GCGC 1 cut(s) 224
AspS9I GGNCC 2 cut(s) 91, 160
AvaII GGWCC 1 cut(s) 160
BanI GGYRCC 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 195
BbvI GCAGC 1 cut(s) 34
BccI CCATC 1 cut(s) 259
BciT130I CCWGG 1 cut(s) 83
BcoDI GTCTC 3 cut(s) 104, 234, 265
BfaI CTAG 1 cut(s) 255
BfoI RGCGCY 1 cut(s) 225
BglI GCCNNNNNGGC 1 cut(s) 219
BisI GCNGC 1 cut(s) 48
BlsI GCNGC 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 160
BmgT120I GGNCC 2 cut(s) 91, 160
BmiI GGNNCC 2 cut(s) 93, 223
BmrFI CCNGG 1 cut(s) 83
BpiI GAAGAC 1 cut(s) 195
Bpu10I CCTNAGC 2 cut(s) 156, 164
BpuEI CTTGAG 1 cut(s) 200
Bsa29I ATCGAT 1 cut(s) 309
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 81
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 154
BseBI CCWGG 1 cut(s) 83
BseCI ATCGAT 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMI GCAATG 1 cut(s) 154
BseMII CTCAG 2 cut(s) 149, 155
BseXI GCAGC 1 cut(s) 34
BsgI GTGCAG 1 cut(s) 33
BshFI GGCC 2 cut(s) 92, 285
BshNI GGYRCC 1 cut(s) 221
BshVI ATCGAT 1 cut(s) 309
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 3 cut(s) 104, 234, 265
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 2 cut(s) 92, 285
Bsp143I GATC 1 cut(s) 310
BspANI GGCC 2 cut(s) 92, 285
BspCNI CTCAG 2 cut(s) 148, 156
BspDI ATCGAT 1 cut(s) 309
BspLI GGNNCC 2 cut(s) 93, 223
BspQI GCTCTTC 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 1 cut(s) 310
BssNI GRCGYC 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 83
Bst6I CTCTTC 2 cut(s) 38, 120
BstACI GRCGYC 1 cut(s) 222
BstAPI GCANNNNNTGC 1 cut(s) 56
BstC8I GCNNGC 2 cut(s) 7, 90
BstDEI CTNAG 4 cut(s) 22, 135, 156, 164
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 1 cut(s) 313
BstMAI GTCTC 3 cut(s) 104, 234, 265
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 3 cut(s) 23, 56, 219
BstNI CCWGG 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 81
BstV1I GCAGC 1 cut(s) 34
BstV2I GAAGAC 1 cut(s) 195
Bsu15I ATCGAT 1 cut(s) 309
BsuRI GGCC 2 cut(s) 92, 285
BsuTUI ATCGAT 1 cut(s) 309
Cac8I GCNNGC 2 cut(s) 7, 90
CaiI CAGNNNCTG 1 cut(s) 88
CfoI GCGC 1 cut(s) 224
Cfr13I GGNCC 2 cut(s) 91, 160
ClaI ATCGAT 1 cut(s) 309
Csp6I GTAC 1 cut(s) 117
CspCI CAANNNNNGTGG 2 cut(s) 262, 297
CviAII CATG 1 cut(s) 66
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 4 cut(s) 22, 135, 156, 164
DinI GGCGCC 1 cut(s) 223
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
Eam1104I CTCTTC 2 cut(s) 38, 120
EarI CTCTTC 2 cut(s) 38, 120
Eco47I GGWCC 1 cut(s) 160
Eco57I CTGAAG 2 cut(s) 144, 308
EcoO109I RGGNCCY 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 81
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
FaeI CATG 1 cut(s) 69
FaiI YATR 5 cut(s) 67, 77, 203, 205, 246
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FatI CATG 1 cut(s) 65
FauNDI CATATG 1 cut(s) 203
Fnu4HI GCNGC 1 cut(s) 48
Fsp4HI GCNGC 1 cut(s) 48
FspBI CTAG 1 cut(s) 255
GlaI GCGC 1 cut(s) 223
GluI GCNGC 1 cut(s) 48
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 2 cut(s) 92, 285
HhaI GCGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 1 cut(s) 69
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HinfI GANTC 1 cut(s) 258
Hpy188III TCNNGA 1 cut(s) 255
HpyAV CCTTC 3 cut(s) 72, 106, 308
HpyCH4V TGCA 3 cut(s) 17, 50, 147
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 56, 219
HpyF3I CTNAG 4 cut(s) 22, 135, 156, 164
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 1 cut(s) 69
HspAI GCGC 1 cut(s) 222
KasI GGCGCC 1 cut(s) 221
Kzo9I GATC 1 cut(s) 310
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 3 cut(s) 64, 85, 332
LpnPI CCDG 7 cut(s) 68, 74, 95, 122, 176, 221, 299
Lsp1109I GCAGC 1 cut(s) 34
MaeI CTAG 1 cut(s) 255
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 4 cut(s) 25, 28, 137, 200
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 1 cut(s) 267
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 2 cut(s) 224, 312
MslI CAYNNNNRTG 1 cut(s) 107
MspA1I CMGCKG 1 cut(s) 47
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 1 cut(s) 83
MwoI GCNNNNNNNGC 3 cut(s) 23, 56, 219
NarI GGCGCC 1 cut(s) 222
NdeI CATATG 1 cut(s) 203
NdeII GATC 1 cut(s) 310
NlaIII CATG 1 cut(s) 69
NlaIV GGNNCC 2 cut(s) 93, 223
OliI CACNNNNGTG 1 cut(s) 107
PciSI GCTCTTC 1 cut(s) 120
PctI GAATGC 1 cut(s) 17
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 1 cut(s) 49
PleI GAGTC 1 cut(s) 266
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 266
PpuMI RGGWCCY 1 cut(s) 160
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 2 cut(s) 93, 223
PspPI GGNCC 2 cut(s) 91, 160
PspPPI RGGWCCY 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
RseI CAYNNNNRTG 1 cut(s) 107
SapI GCTCTTC 1 cut(s) 120
SatI GCNGC 1 cut(s) 48
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 2 cut(s) 91, 160
SchI GAGTC 1 cut(s) 267
ScrFI CCNGG 1 cut(s) 83
SetI ASST 6 cut(s) 49, 90, 132, 165, 294, 300
SfoI GGCGCC 1 cut(s) 223
SinI GGWCC 1 cut(s) 160
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SspDI GGCGCC 1 cut(s) 221
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 81
TaqI TCGA 2 cut(s) 294, 309
TseI GCWGC 1 cut(s) 47
TspDTI ATGAA 1 cut(s) 54
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 160
XbaI TCTAGA 1 cut(s) 254
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.