MD07G1155900.v1.1

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0017362
Physical Location & Seq
Reverse (-)
22679705 .. 22682778
3074 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1155900.v1.1.491

Sequence Viewer

Length: 897 bp
ATGAACGACCTCTCTAATGGCGTCCTGAGAGACCACGGTGGCAGAGCCTTGAAGCCCATTCCGTCTCTGGTTCTCTACATTCTCGTACCCATCTTTTTTCTGGGCCTCTCCGTCTCAATCTTCATCCTCATTGTCGTCCGCAACGCCCTCTTCTTCGTCTTTTTCCTCCTGCTCTCCGCCCTCGTCGCCGCCTTCGTCGTTTGGAATGTACGGCACTGGGCGACCAAAGCTGCCGTCTTTTGGTTCCTCGACTCGGTTCCGGAGTCCGACCTCCGCCTCGCCCAGCACGGACAGCTCGTCAAAATTACTGGGATTGCATCATGTGGGAGTCTATCCCTTGAATCTTCATATGAAAAAGCTACCGGCTGTATCTATGCCTCTACTCTTTTATATGAATACAGGGGATTAACTCTACAGCCAGGGAATGTCAAGAGATCATGCTTCCAGTGGCATTTAGCATATTGTGAGAGATTCTCCACAGACTTTTACTTAACCGATCAAAAGTCTGGTCTTACAGCTATTGTCAAAGCTGGTTCTGGTTGCAAAGTTATCCCACTAATTGTCGAGAGCAAACTTGTCAACACTAAAAGATGCAGAATCCTCTCTCCTCACTTGAGGAAATGGTTAACTGAGAGAAATCTCTCATCTGAATCCCGTCTACTACGTCTGGAGGAAGGGTATGTTCAAGAAGGCAGCTCAGTGACAGTATTCGGAATGTTGCATAGAAACAATGAGATGACTACAATTGTTCAACCGCCAGAAGTAATCTCAACCGGGTGTCTCTGGCGAAAACTGCTTCTCCCAGTTGACGTTGATGGGATGATTCTAAGGGTCTATCAAACGGCCGCTGGGCATTCAATCAACCAAGATTCAATACAACAGGCAGAAAGATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

299

Amino Acids

33.47

Weight (kDa)

9.07

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017362)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17310
malus_domestica MD07G1155900.v1.1
prunus_persica Prupe.2G194300_v2.0.a1
pyrus_communis pycom07g15290
rosa_chinensis RchiOBHm_Chr1g0360741
rosa_laevigata RLG00000027789
rosa_multiflora Rmu_ssc0000022.1_g000012
rosa_roxburghii Rroxscaffold_4G00295860
rosa_rugosa Rorug01G0281300
rosa_samantha Rh1AG293300 Rh1BG257700 Rh1DG287800
rosa_wichuraiana Rw1G025940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 658
AccIII TCCGGA 1 cut(s) 259
AciI CCGC 6 cut(s) 139, 177, 189, 274, 755, 846
AcoI YGGCCR 1 cut(s) 843
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 2 cut(s) 87, 210
AfiI CCNNNNNNNGG 2 cut(s) 240, 253
AgsI TTSAA 6 cut(s) 52, 341, 686, 752, 858, 873
AhdI GACNNNNNGTC 1 cut(s) 296
AjnI CCWGG 1 cut(s) 418
AluBI AGCT 6 cut(s) 230, 295, 359, 518, 530, 696
AluI AGCT 6 cut(s) 230, 295, 359, 518, 530, 696
Alw26I GTCTC 4 cut(s) 24, 69, 118, 785
Aor13HI TCCGGA 1 cut(s) 259
AoxI GGCC 2 cut(s) 103, 843
ApeKI GCWGC 2 cut(s) 230, 693
ArsI GACNNNNNNTTYG 4 cut(s) 219, 251, 493, 525
AspS9I GGNCC 1 cut(s) 103
AsuC2I CCSGG 1 cut(s) 775
BbvI GCAGC 2 cut(s) 217, 705
BccI CCATC 3 cut(s) 98, 809, 885
BceAI ACGGC 3 cut(s) 218, 227, 858
BciT130I CCWGG 1 cut(s) 420
BcnI CCSGG 1 cut(s) 775
BcoDI GTCTC 4 cut(s) 24, 69, 118, 785
BfmI CTRYAG 1 cut(s) 413
BisI GCNGC 4 cut(s) 189, 231, 694, 846
BlsI GCNGC 4 cut(s) 190, 232, 695, 847
Bme1390I CCNGG 2 cut(s) 420, 775
BmeRI GACNNNNNGTC 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 103
BmiI GGNNCC 2 cut(s) 245, 258
BmrFI CCNGG 2 cut(s) 420, 775
BmrI ACTGGG 3 cut(s) 226, 318, 797
BmsI GCATC 2 cut(s) 326, 581
BmuI ACTGGG 3 cut(s) 226, 318, 797
BplI GAGNNNNNCTC 2 cut(s) 458, 490
BpmI CTGGAG 1 cut(s) 689
BpuEI CTTGAG 1 cut(s) 634
BpuMI CCSGG 1 cut(s) 775
BsaHI GRCGYC 1 cut(s) 21
BsaI GGTCTC 1 cut(s) 24
BsaJI CCNNGG 2 cut(s) 34, 419
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 4 cut(s) 662, 692, 783, 813
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 253
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 4 cut(s) 221, 313, 445, 803
BseAI TCCGGA 1 cut(s) 259
BseBI CCWGG 1 cut(s) 420
BseDI CCNNGG 2 cut(s) 34, 419
BseGI GGATG 2 cut(s) 123, 825
BseLI CCNNNNNNNGG 2 cut(s) 240, 253
BseMII CTCAG 3 cut(s) 17, 621, 711
BseNI ACTGG 4 cut(s) 221, 313, 445, 803
BseRI GAGGAG 1 cut(s) 597
BseX3I CGGCCG 1 cut(s) 843
BseXI GCAGC 2 cut(s) 217, 705
BseYI CCCAGC 2 cut(s) 282, 848
Bsh1285I CGRYCG 1 cut(s) 846
BshFI GGCC 2 cut(s) 105, 845
BsiEI CGRYCG 1 cut(s) 846
BsiSI CCGG 3 cut(s) 260, 363, 774
BslI CCNNNNNNNGG 2 cut(s) 240, 253
BsmAI GTCTC 4 cut(s) 24, 69, 118, 785
BsmBI CGTCTC 2 cut(s) 69, 118
BsmI GAATGC 1 cut(s) 853
BsnI GGCC 2 cut(s) 105, 845
Bso31I GGTCTC 1 cut(s) 24
Bsp13I TCCGGA 1 cut(s) 259
Bsp143I GATC 2 cut(s) 434, 496
BspACI CCGC 6 cut(s) 139, 177, 189, 274, 755, 846
BspANI GGCC 2 cut(s) 105, 845
BspCNI CTCAG 3 cut(s) 18, 622, 710
BspEI TCCGGA 1 cut(s) 259
BspLI GGNNCC 2 cut(s) 245, 258
BspTNI GGTCTC 1 cut(s) 24
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 4 cut(s) 221, 313, 445, 803
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 2 cut(s) 34, 419
BssMI GATC 2 cut(s) 434, 496
BssNI GRCGYC 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 2 cut(s) 38, 706
Bst6I CTCTTC 1 cut(s) 155
BstACI GRCGYC 1 cut(s) 21
BstDEI CTNAG 4 cut(s) 26, 630, 697, 827
BstDSI CCRYGG 1 cut(s) 34
BstF5I GGATG 2 cut(s) 123, 825
BstKTI GATC 2 cut(s) 437, 499
BstMAI GTCTC 4 cut(s) 24, 69, 118, 785
BstMBI GATC 2 cut(s) 434, 496
BstMCI CGRYCG 1 cut(s) 846
BstMWI GCNNNNNNNGC 4 cut(s) 185, 227, 292, 793
BstNI CCWGG 1 cut(s) 420
BstSCI CCNGG 2 cut(s) 418, 773
BstSFI CTRYAG 1 cut(s) 413
BstV1I GCAGC 2 cut(s) 217, 705
BstZI CGGCCG 1 cut(s) 843
BsuRI GGCC 2 cut(s) 105, 845
BtgI CCRYGG 1 cut(s) 34
BtsCI GGATG 2 cut(s) 123, 825
BtsIMutI CAGTG 3 cut(s) 214, 452, 705
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 103
CseI GACGC 1 cut(s) 10
Csp6I GTAC 2 cut(s) 86, 209
CviAII CATG 2 cut(s) 321, 438
CviQI GTAC 2 cut(s) 86, 209
DdeI CTNAG 4 cut(s) 26, 630, 697, 827
DpnI GATC 2 cut(s) 436, 498
DpnII GATC 2 cut(s) 434, 496
DriI GACNNNNNGTC 1 cut(s) 296
EaeI YGGCCR 1 cut(s) 843
EagI CGGCCG 1 cut(s) 843
Eam1104I CTCTTC 1 cut(s) 155
Eam1105I GACNNNNNGTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 155
EciI GGCGGA 2 cut(s) 166, 263
EclXI CGGCCG 1 cut(s) 843
Eco31I GGTCTC 1 cut(s) 24
Eco52I CGGCCG 1 cut(s) 843
EcoRII CCWGG 1 cut(s) 418
Esp3I CGTCTC 2 cut(s) 69, 118
FaeI CATG 2 cut(s) 324, 441
FatI CATG 2 cut(s) 320, 437
FauNDI CATATG 1 cut(s) 349
FblI GTMKAC 1 cut(s) 658
Fnu4HI GCNGC 4 cut(s) 189, 231, 694, 846
FokI GGATG 2 cut(s) 110, 832
Fsp4HI GCNGC 4 cut(s) 189, 231, 694, 846
GluI GCNGC 4 cut(s) 189, 231, 694, 846
GsaI CCCAGC 2 cut(s) 286, 852
GsuI CTGGAG 1 cut(s) 689
HaeIII GGCC 2 cut(s) 105, 845
HapII CCGG 3 cut(s) 260, 363, 774
HgaI GACGC 1 cut(s) 10
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 2 cut(s) 324, 441
HincII GTYRAC 3 cut(s) 580, 627, 808
HindII GTYRAC 3 cut(s) 580, 627, 808
HinfI GANTC 9 cut(s) 251, 263, 328, 341, 471, 597, 650, 823, 869
HpaI GTTAAC 1 cut(s) 627
HpaII CCGG 3 cut(s) 260, 363, 774
Hpy166II GTNNAC 4 cut(s) 580, 627, 659, 808
Hpy188I TCNGA 3 cut(s) 268, 649, 713
Hpy188III TCNNGA 6 cut(s) 25, 260, 430, 565, 668, 686
Hpy8I GTNNAC 4 cut(s) 580, 627, 659, 808
Hpy99I CGWCG 2 cut(s) 188, 200
HpyAV CCTTC 3 cut(s) 202, 668, 683
HpyCH4III ACNGT 2 cut(s) 38, 706
HpyCH4IV ACGT 2 cut(s) 664, 810
HpyCH4V TGCA 4 cut(s) 317, 543, 594, 721
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 227, 292, 793
HpyF3I CTNAG 4 cut(s) 26, 630, 697, 827
HpySE526I ACGT 2 cut(s) 664, 810
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 2 cut(s) 324, 441
Kpn2I TCCGGA 1 cut(s) 259
KspAI GTTAAC 1 cut(s) 627
Kzo9I GATC 2 cut(s) 434, 496
Lsp1109I GCAGC 2 cut(s) 217, 705
LweI GCATC 2 cut(s) 326, 581
MaeII ACGT 2 cut(s) 664, 810
MaeIII GTNAC 1 cut(s) 700
MalI GATC 2 cut(s) 436, 498
MboI GATC 2 cut(s) 434, 496
MboII GAAGA 4 cut(s) 112, 142, 145, 336
MfeI CAATTG 1 cut(s) 744
MluCI AATT 3 cut(s) 303, 558, 744
MlyI GAGTC 3 cut(s) 245, 272, 337
MmeI TCCRAC 1 cut(s) 291
MroI TCCGGA 1 cut(s) 259
MseI TTAA 3 cut(s) 407, 491, 626
MspA1I CMGCKG 1 cut(s) 848
MspI CCGG 3 cut(s) 260, 363, 774
MspR9I CCNGG 2 cut(s) 420, 775
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 1 cut(s) 853
MvaI CCWGG 1 cut(s) 420
MwoI GCNNNNNNNGC 4 cut(s) 185, 227, 292, 793
NciI CCSGG 1 cut(s) 775
NdeI CATATG 1 cut(s) 349
NdeII GATC 2 cut(s) 434, 496
NlaIII CATG 2 cut(s) 324, 441
NlaIV GGNNCC 2 cut(s) 245, 258
NmuCI GTSAC 1 cut(s) 700
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 294
PctI GAATGC 1 cut(s) 853
PfeI GAWTC 6 cut(s) 341, 471, 597, 650, 823, 869
PkrI GCNGC 4 cut(s) 190, 232, 695, 847
PleI GAGTC 3 cut(s) 245, 271, 336
PpsI GAGTC 3 cut(s) 245, 271, 336
Psp6I CCWGG 1 cut(s) 418
PspFI CCCAGC 2 cut(s) 282, 848
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 2 cut(s) 245, 258
PspPI GGNCC 1 cut(s) 103
RsaI GTAC 2 cut(s) 87, 210
RsaNI GTAC 2 cut(s) 86, 209
SaqAI TTAA 3 cut(s) 407, 491, 626
SatI GCNGC 4 cut(s) 189, 231, 694, 846
Sau3AI GATC 2 cut(s) 434, 496
Sau96I GGNCC 1 cut(s) 103
SchI GAGTC 3 cut(s) 245, 272, 337
ScrFI CCNGG 2 cut(s) 420, 775
SfaNI GCATC 2 cut(s) 326, 581
SfcI CTRYAG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 613
SmoI CTYRAG 1 cut(s) 613
Sse9I AATT 3 cut(s) 303, 558, 744
SsiI CCGC 6 cut(s) 139, 177, 189, 274, 755, 846
StyD4I CCNGG 2 cut(s) 418, 773
TaaI ACNGT 2 cut(s) 38, 706
TaiI ACGT 2 cut(s) 667, 813
TaqI TCGA 2 cut(s) 249, 564
TasI AATT 3 cut(s) 303, 558, 744
TauI GCSGC 2 cut(s) 191, 848
TfiI GAWTC 6 cut(s) 341, 471, 597, 650, 823, 869
Tru1I TTAA 3 cut(s) 407, 491, 626
Tru9I TTAA 3 cut(s) 407, 491, 626
TscAI CASTG 3 cut(s) 221, 452, 705
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 2 cut(s) 230, 693
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 5 cut(s) 17, 112, 336, 366, 408
TspGWI ACGGA 3 cut(s) 51, 100, 303
TspRI CASTG 3 cut(s) 221, 452, 705
XcmI CCANNNNNNNNNTGG 2 cut(s) 64, 97
XmiI GTMKAC 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.