Rmu_ssc0000022.1_g000012

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000022.1
OG0017362
Physical Location & Seq
Reverse (-)
66120 .. 68720
2601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000022.1_g000012.1.cds

Sequence Viewer

Length: 891 bp
atgaacgacctctcaaacgcggtgctgagagaccaccatactagtaccttcaagcccataccatcccttgtcctctacattctcatacccatctttgttttaggtctctctgtctccatcttcattctcattgccgtccgcaacgccctcttcttcgtcttcttccttttgctctctgctctcgtcctctctttcattctctggaacacgcgtcattgggctactaaagctgcagtcttgttctttctcaactctcttcctgagtctgaccttcgcgtcgctcagcatggagagctggtcaagatcactgggcttgtctcatgcgggagtctctccctggaatcttcatatgaaaaggctactagatgtgtatatgcatctactcttttatatgcatacaagggattaactcttcaacctagaaatgtcaagagatcatgcttccagtggcacttagaatattgtgagagattctcaacagactttttcttgactgatcggaaatctggtcttagagctattgtcaaagctggttctggttgtaatcttatcccactgatctttgagtgcaaacttgtcaacacaagaaaatgcagaatcctttctcctcacttgacgaaatggttacgtgagagaaatctctcagctgaatcccgtctgctacgtctggaggaagggtatgtacaagaaggaagcactgtgactgtatttggcatgctgcacagaaataatgagatgactacgattgttcaaccaccggaagtaatttccagtggttgtctatggcgaaaactgcttctccctgttgacattgatggactcattctaagggtctctctgatggctgggcaatctgtcaaccaaaattctatacaacacccagaaagatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

296

Amino Acids

33.54

Weight (kDa)

9.35

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017362)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17310
malus_domestica MD07G1155900.v1.1
prunus_persica Prupe.2G194300_v2.0.a1
pyrus_communis pycom07g15290
rosa_chinensis RchiOBHm_Chr1g0360741
rosa_laevigata RLG00000027789
rosa_multiflora Rmu_ssc0000022.1_g000012
rosa_roxburghii Rroxscaffold_4G00295860
rosa_rugosa Rorug01G0281300
rosa_samantha Rh1AG293300 Rh1BG257700 Rh1DG287800
rosa_wichuraiana Rw1G025940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
AccII CGCG 3 cut(s) 20, 211, 276
AciI CCGC 3 cut(s) 20, 139, 324
AcsI RAATTY 1 cut(s) 865
AfaI GTAC 2 cut(s) 46, 684
AflIII ACRYGT 1 cut(s) 209
AgsI TTSAA 3 cut(s) 52, 416, 752
AhlI ACTAGT 1 cut(s) 41
AjnI CCWGG 1 cut(s) 336
AluBI AGCT 5 cut(s) 230, 295, 518, 530, 647
AluI AGCT 5 cut(s) 230, 295, 518, 530, 647
Alw26I GTCTC 6 cut(s) 24, 110, 118, 322, 335, 838
ApeKI GCWGC 2 cut(s) 230, 718
ApoI RAATTY 1 cut(s) 865
Asp700I GAANNNNTTC 1 cut(s) 601
BarI GAAGNNNNNNTAC 2 cut(s) 666, 698
BbsI GAAGAC 1 cut(s) 151
BbvI GCAGC 2 cut(s) 217, 705
BccI CCATC 5 cut(s) 70, 98, 125, 809, 835
BceAI ACGGC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 338
BcoDI GTCTC 6 cut(s) 24, 110, 118, 322, 335, 838
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 3 cut(s) 42, 363, 420
BfmI CTRYAG 1 cut(s) 231
BisI GCNGC 2 cut(s) 231, 719
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 2 cut(s) 232, 720
Bme1390I CCNGG 1 cut(s) 338
BmrFI CCNGG 1 cut(s) 338
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 386
BmuI ACTGGG 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 151
BplI GAGNNNNNCTC 2 cut(s) 458, 490
BpmI CTGGAG 1 cut(s) 689
Bpu1102I GCTNAGC 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 629
BsaI GGTCTC 3 cut(s) 24, 110, 838
BsaJI CCNNGG 1 cut(s) 336
BsaWI WCCGGW 1 cut(s) 757
BsaXI ACNNNNNCTCC 6 cut(s) 282, 312, 662, 692, 783, 813
Bse1I ACTGG 3 cut(s) 313, 445, 771
Bse3DI GCAATG 1 cut(s) 129
BseBI CCWGG 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 336
BseGI GGATG 1 cut(s) 62
BseMI GCAATG 1 cut(s) 129
BseMII CTCAG 4 cut(s) 17, 252, 296, 657
BseNI ACTGG 3 cut(s) 313, 445, 771
BseRI GAGGAG 1 cut(s) 597
BseXI GCAGC 2 cut(s) 217, 705
BseYI CCCAGC 1 cut(s) 845
BsgI GTGCAG 1 cut(s) 704
Bsh1236I CGCG 3 cut(s) 20, 211, 276
BsiSI CCGG 1 cut(s) 758
BsmAI GTCTC 6 cut(s) 24, 110, 118, 322, 335, 838
Bso31I GGTCTC 3 cut(s) 24, 110, 838
Bsp1407I TGTACA 1 cut(s) 682
Bsp143I GATC 4 cut(s) 303, 434, 496, 558
Bsp1720I GCTNAGC 1 cut(s) 282
BspACI CCGC 3 cut(s) 20, 139, 324
BspCNI CTCAG 4 cut(s) 18, 253, 295, 656
BspFNI CGCG 3 cut(s) 20, 211, 276
BspMAI CTGCAG 1 cut(s) 235
BspTNI GGTCTC 3 cut(s) 24, 110, 838
BsrDI GCAATG 1 cut(s) 129
BsrGI TGTACA 1 cut(s) 682
BsrI ACTGG 3 cut(s) 313, 445, 771
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 4 cut(s) 303, 434, 496, 558
Bst2UI CCWGG 1 cut(s) 338
Bst4CI ACNGT 2 cut(s) 700, 706
Bst6I CTCTTC 3 cut(s) 155, 261, 417
BstAUI TGTACA 1 cut(s) 682
BstBAI YACGTR 1 cut(s) 629
BstC8I GCNNGC 1 cut(s) 716
BstDEI CTNAG 7 cut(s) 26, 261, 282, 454, 512, 643, 827
BstF5I GGATG 1 cut(s) 62
BstFNI CGCG 3 cut(s) 20, 211, 276
BstKTI GATC 4 cut(s) 306, 437, 499, 561
BstMAI GTCTC 6 cut(s) 24, 110, 118, 322, 335, 838
BstMBI GATC 4 cut(s) 303, 434, 496, 558
BstMWI GCNNNNNNNGC 3 cut(s) 227, 292, 793
BstNI CCWGG 1 cut(s) 338
BstNSI RCATGY 1 cut(s) 718
BstSCI CCNGG 1 cut(s) 336
BstSFI CTRYAG 1 cut(s) 231
BstUI CGCG 3 cut(s) 20, 211, 276
BstV1I GCAGC 2 cut(s) 217, 705
BstV2I GAAGAC 1 cut(s) 151
BtsCI GGATG 1 cut(s) 62
BtsIMutI CAGTG 5 cut(s) 306, 452, 554, 696, 778
Cac8I GCNNGC 1 cut(s) 716
CseI GACGC 2 cut(s) 200, 265
Csp6I GTAC 2 cut(s) 45, 683
CviAII CATG 4 cut(s) 287, 321, 438, 715
CviQI GTAC 2 cut(s) 45, 683
DdeI CTNAG 7 cut(s) 26, 261, 282, 454, 512, 643, 827
DpnI GATC 4 cut(s) 305, 436, 498, 560
DpnII GATC 4 cut(s) 303, 434, 496, 558
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
Eam1104I CTCTTC 3 cut(s) 155, 261, 417
EarI CTCTTC 3 cut(s) 155, 261, 417
Eco31I GGTCTC 3 cut(s) 24, 110, 838
EcoRII CCWGG 1 cut(s) 336
EcoT22I ATGCAT 2 cut(s) 379, 397
FaeI CATG 4 cut(s) 290, 324, 441, 718
FatI CATG 4 cut(s) 286, 320, 437, 714
FauI CCCGC 1 cut(s) 317
FauNDI CATATG 1 cut(s) 349
Fnu4HI GCNGC 2 cut(s) 231, 719
FokI GGATG 1 cut(s) 49
Fsp4HI GCNGC 2 cut(s) 231, 719
FspBI CTAG 3 cut(s) 42, 363, 420
GluI GCNGC 2 cut(s) 231, 719
GsaI CCCAGC 1 cut(s) 849
GsuI CTGGAG 1 cut(s) 689
HapII CCGG 1 cut(s) 758
HgaI GACGC 2 cut(s) 200, 265
Hin1II CATG 4 cut(s) 290, 324, 441, 718
HincII GTYRAC 3 cut(s) 580, 808, 859
HindII GTYRAC 3 cut(s) 580, 808, 859
HinfI GANTC 7 cut(s) 263, 328, 341, 471, 597, 650, 819
HpaII CCGG 1 cut(s) 758
Hpy166II GTNNAC 3 cut(s) 580, 808, 859
Hpy188I TCNGA 3 cut(s) 268, 501, 840
Hpy188III TCNNGA 6 cut(s) 202, 260, 301, 430, 490, 668
Hpy8I GTNNAC 3 cut(s) 580, 808, 859
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 4 cut(s) 58, 281, 668, 683
HpyCH4III ACNGT 2 cut(s) 700, 706
HpyCH4IV ACGT 2 cut(s) 628, 664
HpyCH4V TGCA 6 cut(s) 233, 377, 395, 570, 594, 721
HpyF10VI GCNNNNNNNGC 3 cut(s) 227, 292, 793
HpyF3I CTNAG 7 cut(s) 26, 261, 282, 454, 512, 643, 827
HpySE526I ACGT 2 cut(s) 628, 664
Hsp92II CATG 4 cut(s) 290, 324, 441, 718
Kzo9I GATC 4 cut(s) 303, 434, 496, 558
Lsp1109I GCAGC 2 cut(s) 217, 705
LweI GCATC 1 cut(s) 386
MaeI CTAG 3 cut(s) 42, 363, 420
MaeII ACGT 2 cut(s) 628, 664
MaeIII GTNAC 2 cut(s) 624, 700
MalI GATC 4 cut(s) 305, 436, 498, 560
MboI GATC 4 cut(s) 303, 434, 496, 558
MboII GAAGA 8 cut(s) 112, 142, 145, 151, 154, 248, 336, 404
MluCI AATT 2 cut(s) 765, 865
MluI ACGCGT 1 cut(s) 209
MlyI GAGTC 3 cut(s) 272, 337, 813
MnlI CCTC 6 cut(s) 20, 83, 158, 197, 618, 664
Mph1103I ATGCAT 2 cut(s) 379, 397
MroXI GAANNNNTTC 1 cut(s) 601
MseI TTAA 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 647
MspI CCGG 1 cut(s) 758
MspR9I CCNGG 1 cut(s) 338
MvaI CCWGG 1 cut(s) 338
MvnI CGCG 3 cut(s) 20, 211, 276
MwoI GCNNNNNNNGC 3 cut(s) 227, 292, 793
NdeI CATATG 1 cut(s) 349
NdeII GATC 4 cut(s) 303, 434, 496, 558
NlaIII CATG 4 cut(s) 290, 324, 441, 718
NmuCI GTSAC 1 cut(s) 700
NsiI ATGCAT 2 cut(s) 379, 397
NspI RCATGY 1 cut(s) 718
PaeI GCATGC 1 cut(s) 718
PdmI GAANNNNTTC 1 cut(s) 601
PfeI GAWTC 4 cut(s) 341, 471, 597, 650
PkrI GCNGC 2 cut(s) 232, 720
PleI GAGTC 3 cut(s) 271, 336, 813
PpsI GAGTC 3 cut(s) 271, 336, 813
Ppu21I YACGTR 1 cut(s) 629
Psp6I CCWGG 1 cut(s) 336
PspFI CCCAGC 1 cut(s) 845
PspGI CCWGG 1 cut(s) 336
PstI CTGCAG 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 2 cut(s) 46, 684
RsaNI GTAC 2 cut(s) 45, 683
SaqAI TTAA 1 cut(s) 407
SatI GCNGC 2 cut(s) 231, 719
Sau3AI GATC 4 cut(s) 303, 434, 496, 558
SchI GAGTC 3 cut(s) 272, 337, 813
ScrFI CCNGG 1 cut(s) 338
SfaNI GCATC 1 cut(s) 386
SfcI CTRYAG 1 cut(s) 231
SpeI ACTAGT 1 cut(s) 41
SphI GCATGC 1 cut(s) 718
Sse9I AATT 2 cut(s) 765, 865
SsiI CCGC 3 cut(s) 20, 139, 324
SspI AATATT 1 cut(s) 461
SspMI CTAG 3 cut(s) 42, 363, 420
StyD4I CCNGG 1 cut(s) 336
TaaI ACNGT 2 cut(s) 700, 706
TaiI ACGT 2 cut(s) 631, 667
TasI AATT 2 cut(s) 765, 865
TatI WGTACW 1 cut(s) 682
TfiI GAWTC 4 cut(s) 341, 471, 597, 650
Tru1I TTAA 1 cut(s) 407
Tru9I TTAA 1 cut(s) 407
TscAI CASTG 5 cut(s) 313, 452, 561, 703, 778
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 2 cut(s) 230, 718
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 5 cut(s) 17, 112, 184, 336, 366
TspRI CASTG 5 cut(s) 313, 452, 561, 703, 778
XapI RAATTY 1 cut(s) 865
XceI RCATGY 1 cut(s) 718
XmnI GAANNNNTTC 1 cut(s) 601
XspI CTAG 3 cut(s) 42, 363, 420
Zsp2I ATGCAT 2 cut(s) 379, 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.