Rroxscaffold_4G00295860

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0017362
Physical Location & Seq
Forward (+)
15922739 .. 15925539
2801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00295860.1

Sequence Viewer

Length: 894 bp
ATGAACGACCTATCAAACGCGGTTCTGAGAGACCACCATACTAGTACCTTCAAGCCCATACCATCCCTTGTCCTCTACATTCTCATACCCATCTTTGCTTTGGGTCTCTCTGTCTCCATCTTCATTCTCATTGCCGTCCACAACGCCCTCTTCTTCGTCTCCTTCCTGTTGCTCTCTGCTTTCGTCCTCTCTTTCATTCTTTGGAACACGCGTCATTGGTCTACTAAAGCTGCAGTCTTGTTCTTTCTCAACTCTCTTCCTGAGTCTGACCTTCGTGTGGCTCAGCATGGGGAGCTGGTCAAGATCACTGGGCTTGTCTCATGCGGGAGTCTCTCCCTGGAATCTTCATATGAAAAGGCTACTAGATGTGTATATGCCTCTACTCTTTTATATGAATACAAGGGATTAACTCTTCAACCTAGAAATGTCAAGAGATCATGCTTCCAGTGGCACTTAGAATATTGTGAGAGATTCTCAACAGACTTTTTCTTGACTGATCGGAAATCTGGTCTTAGAGCTATTGTCAAAGCTGGTTCTGGTTGTAATCTTGTCCCACTGGTCTTTGAGAGCAAACTTGTCAACACAAGAAAATGCAGAATCCTCTCTCCTCACTTGACGAAATGGTTACGTGAGAGAAATCTCTCAGCTGAATCCCGTCTGCTACGTCTGGAGGAAGGGTATGTACAAGAAGGAAGCACTGTGACTGTATTTGGCATGCTGCACAGAAATAATGAGATGACTACGATTGTTCAACCACCGGAAGTAATTTCCAGTGGTTGTCTATGGCGAAAACTGCTTCTCCCTGTTGACATTGATGGACTCATTCTAAGGGTCTCTCCTCCGATGGCTGGGCAATCTGTTAACCAAAATTCTATACAACACCCAGAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

297

Amino Acids

33.58

Weight (kDa)

9.21

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017362)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17310
malus_domestica MD07G1155900.v1.1
prunus_persica Prupe.2G194300_v2.0.a1
pyrus_communis pycom07g15290
rosa_chinensis RchiOBHm_Chr1g0360741
rosa_laevigata RLG00000027789
rosa_multiflora Rmu_ssc0000022.1_g000012
rosa_roxburghii Rroxscaffold_4G00295860
rosa_rugosa Rorug01G0281300
rosa_samantha Rh1AG293300 Rh1BG257700 Rh1DG287800
rosa_wichuraiana Rw1G025940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 221
AccII CGCG 2 cut(s) 20, 211
AciI CCGC 2 cut(s) 20, 324
AcsI RAATTY 1 cut(s) 868
AfaI GTAC 2 cut(s) 46, 684
AfiI CCNNNNNNNGG 2 cut(s) 277, 848
AflIII ACRYGT 1 cut(s) 209
AgsI TTSAA 3 cut(s) 52, 416, 752
AhlI ACTAGT 1 cut(s) 41
AjnI CCWGG 1 cut(s) 336
AluBI AGCT 5 cut(s) 230, 295, 518, 530, 647
AluI AGCT 5 cut(s) 230, 295, 518, 530, 647
Alw26I GTCTC 7 cut(s) 24, 110, 118, 163, 322, 335, 838
ApeKI GCWGC 2 cut(s) 230, 718
ApoI RAATTY 1 cut(s) 868
BarI GAAGNNNNNNTAC 2 cut(s) 666, 698
BbvI GCAGC 2 cut(s) 217, 705
BccI CCATC 5 cut(s) 70, 98, 125, 809, 838
BceAI ACGGC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 338
BcoDI GTCTC 7 cut(s) 24, 110, 118, 163, 322, 335, 838
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 3 cut(s) 42, 363, 420
BfmI CTRYAG 1 cut(s) 231
BisI GCNGC 2 cut(s) 231, 719
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 2 cut(s) 232, 720
Bme1390I CCNGG 1 cut(s) 338
BmrFI CCNGG 1 cut(s) 338
BmrI ACTGGG 1 cut(s) 318
BmuI ACTGGG 1 cut(s) 318
BplI GAGNNNNNCTC 2 cut(s) 458, 490
BpmI CTGGAG 1 cut(s) 689
Bpu1102I GCTNAGC 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 629
BsaI GGTCTC 3 cut(s) 24, 110, 838
BsaJI CCNNGG 1 cut(s) 336
BsaWI WCCGGW 1 cut(s) 757
BsaXI ACNNNNNCTCC 4 cut(s) 662, 692, 783, 813
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 848
Bse1I ACTGG 4 cut(s) 313, 445, 561, 771
Bse3DI GCAATG 1 cut(s) 129
BseBI CCWGG 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 336
BseGI GGATG 1 cut(s) 62
BseLI CCNNNNNNNGG 2 cut(s) 277, 848
BseMI GCAATG 1 cut(s) 129
BseMII CTCAG 4 cut(s) 17, 252, 296, 657
BseNI ACTGG 4 cut(s) 313, 445, 561, 771
BseRI GAGGAG 2 cut(s) 597, 828
BseXI GCAGC 2 cut(s) 217, 705
BseYI CCCAGC 1 cut(s) 848
BsgI GTGCAG 1 cut(s) 704
Bsh1236I CGCG 2 cut(s) 20, 211
BsiSI CCGG 1 cut(s) 758
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 2 cut(s) 277, 848
BsmAI GTCTC 7 cut(s) 24, 110, 118, 163, 322, 335, 838
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 536
Bso31I GGTCTC 3 cut(s) 24, 110, 838
Bsp1407I TGTACA 1 cut(s) 682
Bsp143I GATC 3 cut(s) 303, 434, 496
Bsp1720I GCTNAGC 1 cut(s) 282
BspACI CCGC 2 cut(s) 20, 324
BspCNI CTCAG 4 cut(s) 18, 253, 295, 656
BspFNI CGCG 2 cut(s) 20, 211
BspMAI CTGCAG 1 cut(s) 235
BspTNI GGTCTC 3 cut(s) 24, 110, 838
BsrDI GCAATG 1 cut(s) 129
BsrGI TGTACA 1 cut(s) 682
BsrI ACTGG 4 cut(s) 313, 445, 561, 771
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 3 cut(s) 303, 434, 496
Bst2UI CCWGG 1 cut(s) 338
Bst4CI ACNGT 2 cut(s) 700, 706
Bst6I CTCTTC 3 cut(s) 155, 261, 417
BstAUI TGTACA 1 cut(s) 682
BstBAI YACGTR 1 cut(s) 629
BstC8I GCNNGC 1 cut(s) 716
BstDEI CTNAG 7 cut(s) 26, 261, 282, 454, 512, 643, 827
BstF5I GGATG 1 cut(s) 62
BstFNI CGCG 2 cut(s) 20, 211
BstKTI GATC 3 cut(s) 306, 437, 499
BstMAI GTCTC 7 cut(s) 24, 110, 118, 163, 322, 335, 838
BstMBI GATC 3 cut(s) 303, 434, 496
BstMWI GCNNNNNNNGC 2 cut(s) 292, 793
BstNI CCWGG 1 cut(s) 338
BstNSI RCATGY 1 cut(s) 718
BstSCI CCNGG 1 cut(s) 336
BstSFI CTRYAG 1 cut(s) 231
BstUI CGCG 2 cut(s) 20, 211
BstV1I GCAGC 2 cut(s) 217, 705
BtsCI GGATG 1 cut(s) 62
BtsIMutI CAGTG 5 cut(s) 306, 452, 554, 696, 778
Cac8I GCNNGC 1 cut(s) 716
CseI GACGC 1 cut(s) 200
Csp6I GTAC 2 cut(s) 45, 683
CviAII CATG 4 cut(s) 287, 321, 438, 715
CviQI GTAC 2 cut(s) 45, 683
DdeI CTNAG 7 cut(s) 26, 261, 282, 454, 512, 643, 827
DpnI GATC 3 cut(s) 305, 436, 498
DpnII GATC 3 cut(s) 303, 434, 496
Eam1104I CTCTTC 3 cut(s) 155, 261, 417
EarI CTCTTC 3 cut(s) 155, 261, 417
Eco31I GGTCTC 3 cut(s) 24, 110, 838
EcoRII CCWGG 1 cut(s) 336
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 4 cut(s) 290, 324, 441, 718
FaqI GGGAC 1 cut(s) 536
FatI CATG 4 cut(s) 286, 320, 437, 714
FauI CCCGC 1 cut(s) 317
FauNDI CATATG 1 cut(s) 349
FblI GTMKAC 1 cut(s) 221
Fnu4HI GCNGC 2 cut(s) 231, 719
FokI GGATG 1 cut(s) 49
Fsp4HI GCNGC 2 cut(s) 231, 719
FspBI CTAG 3 cut(s) 42, 363, 420
GluI GCNGC 2 cut(s) 231, 719
GsaI CCCAGC 1 cut(s) 852
GsuI CTGGAG 1 cut(s) 689
HapII CCGG 1 cut(s) 758
HgaI GACGC 1 cut(s) 200
Hin1II CATG 4 cut(s) 290, 324, 441, 718
HincII GTYRAC 3 cut(s) 580, 808, 862
HindII GTYRAC 3 cut(s) 580, 808, 862
HinfI GANTC 7 cut(s) 263, 328, 341, 471, 597, 650, 819
HpaI GTTAAC 1 cut(s) 862
HpaII CCGG 1 cut(s) 758
Hpy166II GTNNAC 5 cut(s) 139, 222, 580, 808, 862
Hpy188I TCNGA 4 cut(s) 27, 268, 501, 843
Hpy188III TCNNGA 5 cut(s) 260, 301, 430, 490, 668
Hpy8I GTNNAC 5 cut(s) 139, 222, 580, 808, 862
HpyAV CCTTC 5 cut(s) 58, 172, 281, 668, 683
HpyCH4III ACNGT 2 cut(s) 700, 706
HpyCH4IV ACGT 2 cut(s) 628, 664
HpyCH4V TGCA 3 cut(s) 233, 594, 721
HpyF10VI GCNNNNNNNGC 2 cut(s) 292, 793
HpyF3I CTNAG 7 cut(s) 26, 261, 282, 454, 512, 643, 827
HpySE526I ACGT 2 cut(s) 628, 664
Hsp92II CATG 4 cut(s) 290, 324, 441, 718
KspAI GTTAAC 1 cut(s) 862
Kzo9I GATC 3 cut(s) 303, 434, 496
LmnI GCTCC 1 cut(s) 292
Lsp1109I GCAGC 2 cut(s) 217, 705
MaeI CTAG 3 cut(s) 42, 363, 420
MaeII ACGT 2 cut(s) 628, 664
MaeIII GTNAC 2 cut(s) 624, 700
MalI GATC 3 cut(s) 305, 436, 498
MboI GATC 3 cut(s) 303, 434, 496
MboII GAAGA 6 cut(s) 112, 142, 145, 248, 336, 404
MluCI AATT 2 cut(s) 765, 868
MluI ACGCGT 1 cut(s) 209
MlyI GAGTC 3 cut(s) 272, 337, 813
MnlI CCTC 8 cut(s) 83, 158, 197, 388, 611, 618, 664, 849
MseI TTAA 2 cut(s) 407, 861
MspA1I CMGCKG 1 cut(s) 647
MspI CCGG 1 cut(s) 758
MspR9I CCNGG 1 cut(s) 338
MvaI CCWGG 1 cut(s) 338
MvnI CGCG 2 cut(s) 20, 211
MwoI GCNNNNNNNGC 2 cut(s) 292, 793
NdeI CATATG 1 cut(s) 349
NdeII GATC 3 cut(s) 303, 434, 496
NlaIII CATG 4 cut(s) 290, 324, 441, 718
NmuCI GTSAC 1 cut(s) 700
NspI RCATGY 1 cut(s) 718
PaeI GCATGC 1 cut(s) 718
PfeI GAWTC 4 cut(s) 341, 471, 597, 650
PkrI GCNGC 2 cut(s) 232, 720
PleI GAGTC 3 cut(s) 271, 336, 813
PpsI GAGTC 3 cut(s) 271, 336, 813
Ppu21I YACGTR 1 cut(s) 629
Psp6I CCWGG 1 cut(s) 336
PspFI CCCAGC 1 cut(s) 848
PspGI CCWGG 1 cut(s) 336
PstI CTGCAG 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 2 cut(s) 46, 684
RsaNI GTAC 2 cut(s) 45, 683
SaqAI TTAA 2 cut(s) 407, 861
SatI GCNGC 2 cut(s) 231, 719
Sau3AI GATC 3 cut(s) 303, 434, 496
SchI GAGTC 3 cut(s) 272, 337, 813
ScrFI CCNGG 1 cut(s) 338
SfcI CTRYAG 1 cut(s) 231
SpeI ACTAGT 1 cut(s) 41
SphI GCATGC 1 cut(s) 718
Sse9I AATT 2 cut(s) 765, 868
SsiI CCGC 2 cut(s) 20, 324
SspI AATATT 1 cut(s) 461
SspMI CTAG 3 cut(s) 42, 363, 420
StyD4I CCNGG 1 cut(s) 336
TaaI ACNGT 2 cut(s) 700, 706
TaiI ACGT 2 cut(s) 631, 667
TasI AATT 2 cut(s) 765, 868
TatI WGTACW 1 cut(s) 682
TfiI GAWTC 4 cut(s) 341, 471, 597, 650
Tru1I TTAA 2 cut(s) 407, 861
Tru9I TTAA 2 cut(s) 407, 861
TscAI CASTG 5 cut(s) 313, 452, 561, 703, 778
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 2 cut(s) 230, 718
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 6 cut(s) 17, 112, 184, 336, 366, 408
TspRI CASTG 5 cut(s) 313, 452, 561, 703, 778
XapI RAATTY 1 cut(s) 868
XceI RCATGY 1 cut(s) 718
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XmiI GTMKAC 1 cut(s) 221
XspI CTAG 3 cut(s) 42, 363, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.