MD07G1172300.v1.1

Adaptor-related protein complex 5, beta 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011344
Physical Location & Seq
Forward (+)
24955525 .. 24960718
5194 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1172300.v1.1.491

Sequence Viewer

Length: 3450 bp
ATGACCGATAAGCCACCAGAAGCACCATCTCCGTCACCGCCGTCACCTCCGCCATTAAGGCAACTCTCTCCCCAAGACTGGGAGTCGTTAATCGACGACTTCCAGCACGGCGGCGCACGCCAGGACAGATGGACCTCCGCTCAGCCGATACTCCTCTCCCTCCTCGACCAAGCCCTCTCCTCCATCGCCAAGCGCGACTTCCCCCTCAAGCTCCACGTCATCACCTTCCTCGAGGAGTTCTCCGACCCGCTATTCTCCACTGCCATCGCTAACGACGCCGTTTCCGGACGCAAAGTCCTCCACCGCCTCATCGAAACCCTACGCGCCCTGATCCAGACACCGCCCGATGGGGTCCACATCACGTTCGCCCTCAAGGAGCAGATGATGATCTCTGTCACCTCCGTCGTCGTCTCTCTCGACGACGACGTCGTTCCGATCTCCACCGTCGAATCGCTGGTGGAGCTGCTGTTAACCGTGATCAACCGTCCCAATCATGGCGTCGACCGTCAGGCGCGTGCCGTGGCGTGCGAGTGCCTGCGCGAGATGGAGAAGGCTCGGCCCTCCTTGCTCTCTGAAATCGGCGGACATCTCTGGAGCTTGTGCCAGAACGAGAGGACTCACGCCGCTCAGAGCTACATTCTGTTGTTCACCACCGTCGTTCACAACATCGTCATCGAAAATCTCAGCGTTTCAATTCTCAATACGACGGCGCCTTTGGTGCCGTTCAGTTCGCCGCAGAGCGGGTCAGGTAAGGAGGGTTCGGGTGGGTTGAATTACAAGGAGCTGAGGAGGGCAATGGCTTTTCTGCTAGAGTGGCCGCAAGTGTTGACGCCGTGTGCGATGGTTGAGTTCTTGGCTCTGGTAATGCCAATGGCGGCGGCATTGGAGCTCCAAGCTTCGATGCTCAAGGTCCAATTTTTTGGGATGGTTTATTCGTCTGATCCTATGCTCTGCCATGTTGTTTTGACCATGTTTCCGAGGTTCTGGGACGCGTTTGATGGGCAGGAAGGCGACATTGCATGGAGGCTTGTGCTGCTATCCAAAGAATCGCAGCACCACTTGGTTTTTCGATTGCTGGCGGTGCATTGGTTGCTTGGTTTTGGTCAGTTAGTGTTGAGAAGGGAAGTAAAGAAAGTGAAGACCATTGTTGATATGGGTTCGAGATTCTATCCGAGTGTGTTCGATCCCCTTGCTTTGAAAGCCTTGAAGCTTGACCTGCTTGCGTTTTGCTCTGTTTGTGTTGATGTCTTGAAACCGGAGAAGAGTGTTTCGGGCGAGGATGGTGAGGTGAATGATAAGTTGGTGGTGAAGTTGTTTGAAGATGGACTTGTTTGTGTATCAGCATTCAAATGGTTGCCTCCGCGAAGTACTGAAACCTCGGTGGCATTTCGAACTTTGCACAGGTTCTTGATTGGAGCTTCGTCTCATTCAGACAATGATCCTTCCACCACCAGAAGTCTCATGGACTCCACCACTTTCAGGAATATACAGGGGATGTTGGTGGACCTGATGTTGGAGTGTCGGAGATTGGTCCCTGTGGTAGTTGTTTTGACTGATCGCTTGTTGGGTTGTCAAAAGCACCGTTGGTTGGGAGAGCGCCTGCTTCAGGCATTTGATGAGCATTTGCTTCCAAAAGTGAAATTGGATTACAGTTTGGTTTCTTTCTTTCCAATATTTGATAGAATTGCTGAAAGTGATACAATTCCTCCTTGTGGATTGCTAGAGCTTCTCACCAAGTTCATGGCCTTCCTTGTTGGTAAACATGGCCCATATACAGGACTAAGATCATGGTCTCAGGGAAGCAGAGTTCTTGGCATCTGTCGAACCTTGTTGATGCATCACAAGAGCTCTCGATTGTTCCTTAGAATGTCTCGCCTTTTGGCATTTACTTGCCTTTACTTTCCTGATTTGGAGGTTCGTGACAATGCAAGGATCTACCTTCGGCTTCTTATCTGTGTGCCGGGAAAGAAGCTCAGGGACCTGCTGAATCTTGGGGAGCAACTCAGTATTTCACCGTCTTCACATGCCAATTTCAATGTCCAGACTCCTCGCTTTTCTCATAGTCTCAAGAAGTCTAAGACTGTCTCTTCATATGTCCACCTTGAGCGCGTAATCCCCCTACTAGTCAAACAGTCTTGGTCCTTGTCTTTATCATCTTTGGGTATTGGAAGTAGTGATCCTGGTTACTTAGGAGGCATCACGGACATTGAACCCATAATAGAGGATAGTGATATTGATGACAGTAGTGATGTCCAGATTGCACCAGAAGCTCAAGCAATTGATGACAATAATAATGTCCAAATTATCCCTGAAGATCGAAGAATTGATCAACCACCGGAGCCATTGCGTGTGACGGATTCAAAGCTTTCGGAGATATTAAGAATGCTGAGGATGCATTTCTCATGCATTCCTGACTTCAGACATATGCCAGGAATTAAGGTTAGACTATCATGTAGTTTGAGGTTTGAATCTGAGCCTTTCAATCGAATATGGGGAGTTGATTGCCATGCTGGTGGTTCGAATGAATTAGATGCCCTTCCTGCTATATATGCAACAGTGCTAAAATTTTCGTCCTCTGCAGCATACGGGTCCATTCCATCATATCATATACCTTTTCTTCTAGGTGAGCCTCCCAGAAAAACTAATATACCTGATCAGACCGCCTCATTAGCTATTGTTCCTGTAGAAAATGCTTGTGGAGAAGAGGAACCGTATAGAGCTCCTGTAACGATTGAATTGGAACCACGGGAACCCACGCCTGGTCTAATTGATGTTTCAATTGAAACAAGTGCTGAAAGTGGTCAGATAATCAGGGGACAGCTTCACACAATCACTGTTGGCATAGAGGATATGTTTCTCAAGGCTATTGTACCACCTGACATCCAGAATGATTCCATACCTGGTTATTACTTAGATTTGTTCAGTGCTCTTTGGGAGGCATGTGGCACTTCAAACACTGCACGCGAGACATTTCAGTTGAAAGGAGGCAAAGGGGTCACTGCTATCAGTGGTACTCGGTCGGTCAAGCTACTTGAAGTCCCTGCATCATCCTTGATTCAAGCTACCGAGCGCTATTTGGCACCCTTCGTTGTAAGTGTGATTGGTGAACCACTTGTTACTACTGTAAAGGAAGGAGGAATCATTAGGGACATAATCTGGAAGGATGAGGCCTCAGATTCGTCCCTTGATATTACTAGCTCCGAGACCAATTTTCATAGAGGCCCACTTCATCTTACATACGGTGATGATGCAGAGGAAAGGGACAGTCCTGTCAATACCCGCAAAACAAATATGGGTAGCTTTCTTATTTTGATATTTCTTCCACCAAGGTATCATCTTCTCTTCCAAATGGAAGTGTCTGATGTTTCAACGTTAGTACGAATTCGAACTGATCACTGGCCGTGCTTAGCGTATACTGATGATTATTTGGAAGCATTATTTTTGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1150

Amino Acids

128.01

Weight (kDa)

5.68

Isoelectric Point (pI)

48.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP5B1_middle PF21588 259 - 658 5.6e-25 AP5B1, middle domain
AP5B1_C PF21590 1097 - 1145 1.3e-07 AP5B1, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19870 AT3G19870
fragaria_vesca FvH4_7g19060
malus_domestica MD01G1105900.v1.1 MD07G1172300.v1.1
prunus_persica Prupe.2G211300_v2.0.a1
pyrus_communis pycom01g13270 pycom07g16670 pycom07g16680
rosa_chinensis RchiOBHm_Chr1g0362961
rosa_laevigata RLG00000027607
rosa_multiflora Rmu_sc0000174.1_g000010
rosa_roxburghii Rroxscaffold_4G00293600
rosa_rugosa Rorug01G0302400
rosa_samantha Rh1AG311900 Rh1BG275100 Rh1CG292200 Rh1DG305800 Rh7CG217100
rosa_wichuraiana Rw1G027650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 425, 3269
AatII GACGTC 1 cut(s) 429
AbsI CCTCGAGG 1 cut(s) 230
Acc36I ACCTGC 2 cut(s) 1224, 1989
AccB1I GGYRCC 3 cut(s) 709, 718, 3079
AccBSI CCGCTC 3 cut(s) 140, 626, 741
AccI GTMKAC 2 cut(s) 501, 3413
AccII CGCG 8 cut(s) 195, 324, 514, 540, 992, 1363, 2109, 2964
AccIII TCCGGA 1 cut(s) 284
AclI AACGTT 1 cut(s) 3371
AclWI GGATC 6 cut(s) 325, 935, 1178, 1433, 1940, 2171
AcoI YGGCCR 2 cut(s) 815, 3398
AcsI RAATTY 2 cut(s) 2564, 3381
AcuI CTGAAG 3 cut(s) 1589, 2331, 2401
AcyI GRCGYC 5 cut(s) 276, 426, 498, 710, 830
AfaI GTAC 4 cut(s) 1369, 2871, 3013, 3378
AfeI AGCGCT 1 cut(s) 3071
AflIII ACRYGT 1 cut(s) 990
AhdI GACNNNNNGTC 2 cut(s) 82, 293
AhlI ACTAGT 1 cut(s) 2122
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 5 cut(s) 120, 2179, 2428, 2758, 2899
AjuI GAANNNNNNNTTGG 4 cut(s) 182, 214, 1283, 1315
AloI GAACNNNNNNTCC 2 cut(s) 1791, 1823
Alw21I GWGCWC 4 cut(s) 891, 1850, 2722, 2929
Alw26I GTCTC 9 cut(s) 415, 1428, 1463, 1797, 1875, 2069, 2089, 2960, 3197
AlwI GGATC 6 cut(s) 325, 935, 1178, 1433, 1940, 2171
Ama87I CYCGRG 1 cut(s) 230
Aor13HI TCCGGA 1 cut(s) 284
Aor51HI AGCGCT 1 cut(s) 3071
AoxI GGCC 7 cut(s) 557, 815, 1743, 1765, 3168, 3220, 3398
ApeKI GCWGC 4 cut(s) 463, 1033, 1051, 2579
ApoI RAATTY 2 cut(s) 2564, 3381
ArsI GACNNNNNNTTYG 2 cut(s) 697, 729
AspLEI GCGC 9 cut(s) 116, 195, 326, 514, 540, 712, 1599, 2109, 3072
AsuC2I CCSGG 1 cut(s) 1962
AsuII TTCGAA 3 cut(s) 1390, 2519, 3385
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 8 cut(s) 132, 352, 910, 1504, 1531, 1978, 2139, 2589
BaeI ACNNNNGTAYC 2 cut(s) 3314, 3347
BanI GGYRCC 3 cut(s) 709, 718, 3079
BanII GRGCYC 3 cut(s) 891, 1850, 2722
BbsI GAAGAC 2 cut(s) 1145, 2010
Bbv12I GWGCWC 4 cut(s) 891, 1850, 2722, 2929
BbvCI CCTCAGC 2 cut(s) 785, 2387
BbvI GCAGC 4 cut(s) 450, 1020, 1063, 2591
BceAI ACGGC 8 cut(s) 25, 124, 263, 503, 706, 723, 817, 3385
BcgI CGANNNNNNTGC 2 cut(s) 1172, 1206
BciT130I CCWGG 5 cut(s) 122, 2181, 2430, 2760, 2901
BclI TGATCA 4 cut(s) 477, 2326, 2653, 3391
BcnI CCSGG 1 cut(s) 1962
BcoDI GTCTC 9 cut(s) 415, 1428, 1463, 1797, 1875, 2069, 2089, 2960, 3197
BcuI ACTAGT 1 cut(s) 2122
BfaI CTAG 5 cut(s) 809, 1721, 2123, 2621, 3195
BfmI CTRYAG 2 cut(s) 2577, 2682
BfoI RGCGCY 3 cut(s) 713, 1600, 3073
BfuAI ACCTGC 2 cut(s) 1224, 1989
BglI GCCNNNNNGGC 1 cut(s) 58
BlpI GCTNAGC 2 cut(s) 141, 3406
BmcAI AGTACT 1 cut(s) 1369
Bme1390I CCNGG 6 cut(s) 122, 1962, 2181, 2430, 2760, 2901
Bme18I GGWCC 8 cut(s) 132, 352, 910, 1504, 1531, 1978, 2139, 2589
BmeRI GACNNNNNGTC 2 cut(s) 82, 293
BmeT110I CYCGRG 1 cut(s) 230
BmgBI CACGTC 1 cut(s) 217
BmrFI CCNGG 6 cut(s) 122, 1962, 2181, 2430, 2760, 2901
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 9 cut(s) 891, 1824, 1824, 1846, 2205, 2382, 2521, 3053, 3238
BmuI ACTGGG 1 cut(s) 88
BpiI GAAGAC 2 cut(s) 1145, 2010
BplI GAGNNNNNCTC 2 cut(s) 224, 256
BpmI CTGGAG 1 cut(s) 613
Bpu10I CCTNAGC 3 cut(s) 785, 1973, 2387
Bpu1102I GCTNAGC 2 cut(s) 141, 3406
Bpu14I TTCGAA 3 cut(s) 1390, 2519, 3385
BpuEI CTTGAG 7 cut(s) 191, 356, 890, 2051, 2123, 2256, 2843
BpuMI CCSGG 1 cut(s) 1962
BsaBI GATNNNNATC 1 cut(s) 386
BsaHI GRCGYC 5 cut(s) 276, 426, 498, 710, 830
BsaI GGTCTC 2 cut(s) 1797, 3197
BsaJI CCNNGG 5 cut(s) 519, 977, 1377, 2744, 3326
BsaWI WCCGGW 3 cut(s) 284, 1255, 2335
BsaXI ACNNNNNCTCC 4 cut(s) 452, 482, 1690, 1720
Bse1I ACTGG 2 cut(s) 83, 3401
Bse3DI GCAATG 3 cut(s) 801, 1014, 2342
Bse8I GATNNNNATC 1 cut(s) 386
BseAI TCCGGA 1 cut(s) 284
BseBI CCWGG 5 cut(s) 122, 2181, 2430, 2760, 2901
BseDI CCNNGG 5 cut(s) 519, 977, 1377, 2744, 3326
BseGI GGATG 7 cut(s) 930, 1285, 1500, 2397, 2880, 3047, 3169
BseJI GATNNNNATC 1 cut(s) 386
BseMI GCAATG 3 cut(s) 801, 1014, 2342
BseNI ACTGG 2 cut(s) 83, 3401
BseRI GAGGAG 6 cut(s) 143, 152, 169, 248, 802, 2037
BseXI GCAGC 4 cut(s) 450, 1020, 1063, 2591
BsgI GTGCAG 1 cut(s) 2943
Bsh1236I CGCG 8 cut(s) 195, 324, 514, 540, 992, 1363, 2109, 2964
Bsh1285I CGRYCG 2 cut(s) 505, 3020
BshFI GGCC 7 cut(s) 559, 817, 1745, 1767, 3170, 3222, 3400
BshNI GGYRCC 3 cut(s) 709, 718, 3079
BsiEI CGRYCG 2 cut(s) 505, 3020
BsiHKAI GWGCWC 4 cut(s) 891, 1850, 2722, 2929
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 4 cut(s) 285, 1256, 1961, 2336
BslFI GGGAC 9 cut(s) 471, 1001, 1517, 1991, 2829, 3023, 3161, 3166, 3275
BsmAI GTCTC 9 cut(s) 415, 1428, 1463, 1797, 1875, 2069, 2089, 2960, 3197
BsmBI CGTCTC 2 cut(s) 415, 1428
BsmFI GGGAC 9 cut(s) 471, 1001, 1517, 1991, 2829, 3023, 3161, 3166, 3275
BsmI GAATGC 3 cut(s) 1343, 2388, 2406
BsnI GGCC 7 cut(s) 559, 817, 1745, 1767, 3170, 3222, 3400
Bso31I GGTCTC 2 cut(s) 1797, 3197
BsoBI CYCGRG 1 cut(s) 230
Bsp119I TTCGAA 3 cut(s) 1390, 2519, 3385
Bsp1286I GDGCHC 4 cut(s) 891, 1850, 2722, 2929
Bsp13I TCCGGA 1 cut(s) 284
Bsp1720I GCTNAGC 2 cut(s) 141, 3406
BspANI GGCC 7 cut(s) 559, 817, 1745, 1767, 3170, 3222, 3400
BspEI TCCGGA 1 cut(s) 284
BspFNI CGCG 8 cut(s) 195, 324, 514, 540, 992, 1363, 2109, 2964
BspMAI CTGCAG 1 cut(s) 2581
BspMI ACCTGC 2 cut(s) 1224, 1989
BspPI GGATC 6 cut(s) 325, 935, 1178, 1433, 1940, 2171
BspT104I TTCGAA 3 cut(s) 1390, 2519, 3385
BspT107I GGYRCC 3 cut(s) 709, 718, 3079
BspTNI GGTCTC 2 cut(s) 1797, 3197
BsrBI CCGCTC 3 cut(s) 140, 626, 741
BsrDI GCAATG 3 cut(s) 801, 1014, 2342
BsrI ACTGG 2 cut(s) 83, 3401
BssECI CCNNGG 5 cut(s) 519, 977, 1377, 2744, 3326
BssNAI GTATAC 1 cut(s) 3414
BssNI GRCGYC 5 cut(s) 276, 426, 498, 710, 830
BssT1I CCWWGG 1 cut(s) 3326
Bst1107I GTATAC 1 cut(s) 3414
Bst2UI CCWGG 5 cut(s) 122, 2181, 2430, 2760, 2901
Bst6I CTCTTC 4 cut(s) 1256, 2092, 2697, 3347
BstACI GRCGYC 5 cut(s) 276, 426, 498, 710, 830
BstAPI GCANNNNNTGC 1 cut(s) 1090
BstBI TTCGAA 3 cut(s) 1390, 2519, 3385
BstC8I GCNNGC 8 cut(s) 118, 516, 526, 536, 1077, 1221, 1601, 2962
BstDSI CCRYGG 2 cut(s) 519, 2744
BstENI CCTNNNNNAGG 1 cut(s) 1604
BstF5I GGATG 7 cut(s) 930, 1285, 1500, 2397, 2880, 3047, 3169
BstFNI CGCG 8 cut(s) 195, 324, 514, 540, 992, 1363, 2109, 2964
BstH2I RGCGCY 3 cut(s) 713, 1600, 3073
BstHHI GCGC 9 cut(s) 116, 195, 326, 514, 540, 712, 1599, 2109, 3072
BstMAI GTCTC 9 cut(s) 415, 1428, 1463, 1797, 1875, 2069, 2089, 2960, 3197
BstMCI CGRYCG 2 cut(s) 505, 3020
BstNI CCWGG 5 cut(s) 122, 2181, 2430, 2760, 2901
BstNSI RCATGY 2 cut(s) 2027, 2943
BstSCI CCNGG 6 cut(s) 120, 1960, 2179, 2428, 2758, 2899
BstSFI CTRYAG 2 cut(s) 2577, 2682
BstUI CGCG 8 cut(s) 195, 324, 514, 540, 992, 1363, 2109, 2964
BstV1I GCAGC 4 cut(s) 450, 1020, 1063, 2591
BstV2I GAAGAC 2 cut(s) 1145, 2010
BstX2I RGATCY 1 cut(s) 1932
BstXI CCANNNNNNTGG 3 cut(s) 920, 1741, 2513
BstYI RGATCY 1 cut(s) 1932
BstZ17I GTATAC 1 cut(s) 3414
BsuRI GGCC 7 cut(s) 559, 817, 1745, 1767, 3170, 3222, 3400
BtgI CCRYGG 2 cut(s) 519, 2744
BtgZI GCGATG 3 cut(s) 169, 250, 854
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 7 cut(s) 930, 1285, 1500, 2397, 2880, 3047, 3169
BtsI GCAGTG 3 cut(s) 258, 2955, 2997
BtsIMutI CAGTG 8 cut(s) 258, 2562, 2832, 2929, 2955, 2997, 3013, 3394
BveI ACCTGC 2 cut(s) 1224, 1989
Cac8I GCNNGC 8 cut(s) 118, 516, 526, 536, 1077, 1221, 1601, 2962
CfoI GCGC 9 cut(s) 116, 195, 326, 514, 540, 712, 1599, 2109, 3072
CseI GACGC 5 cut(s) 284, 297, 487, 838, 998
CsiI ACCWGGT 1 cut(s) 2899
Csp6I GTAC 4 cut(s) 1368, 2870, 3012, 3377
CviQI GTAC 4 cut(s) 1368, 2870, 3012, 3377
DinI GGCGCC 1 cut(s) 711
DrdI GACNNNNNNGTC 2 cut(s) 425, 3269
DriI GACNNNNNGTC 2 cut(s) 82, 293
DseDI GACNNNNNNGTC 2 cut(s) 425, 3269
EaeI YGGCCR 2 cut(s) 815, 3398
Eam1104I CTCTTC 4 cut(s) 1256, 2092, 2697, 3347
Eam1105I GACNNNNNGTC 2 cut(s) 82, 293
EarI CTCTTC 4 cut(s) 1256, 2092, 2697, 3347
EciI GGCGGA 2 cut(s) 39, 597
Ecl136II GAGCTC 3 cut(s) 889, 1848, 2720
Eco130I CCWWGG 1 cut(s) 3326
Eco147I AGGCCT 1 cut(s) 3170
Eco24I GRGCYC 3 cut(s) 891, 1850, 2722
Eco31I GGTCTC 2 cut(s) 1797, 3197
Eco47I GGWCC 8 cut(s) 132, 352, 910, 1504, 1531, 1978, 2139, 2589
Eco47III AGCGCT 1 cut(s) 3071
Eco53kI GAGCTC 3 cut(s) 889, 1848, 2720
Eco57I CTGAAG 3 cut(s) 1589, 2331, 2401
Eco88I CYCGRG 1 cut(s) 230
EcoICRI GAGCTC 3 cut(s) 889, 1848, 2720
EcoNI CCTNNNNNAGG 1 cut(s) 1604
EcoO109I RGGNCCY 1 cut(s) 1978
EcoRI GAATTC 1 cut(s) 3381
EcoRII CCWGG 5 cut(s) 120, 2179, 2428, 2758, 2899
EcoT14I CCWWGG 1 cut(s) 3326
EcoT22I ATGCAT 3 cut(s) 1839, 2397, 2408
EcoT38I GRGCYC 3 cut(s) 891, 1850, 2722
EgeI GGCGCC 1 cut(s) 711
EheI GGCGCC 1 cut(s) 711
ErhI CCWWGG 1 cut(s) 3326
Esp3I CGTCTC 2 cut(s) 415, 1428
FalI AAGNNNNNCTT 4 cut(s) 182, 214, 1311, 1343
FaqI GGGAC 9 cut(s) 471, 1001, 1517, 1991, 2829, 3023, 3161, 3166, 3275
FauI CCCGC 3 cut(s) 255, 734, 3287
FauNDI CATATG 2 cut(s) 2092, 2424
FbaI TGATCA 4 cut(s) 477, 2326, 2653, 3391
FblI GTMKAC 2 cut(s) 501, 3413
FokI GGATG 7 cut(s) 937, 1292, 1507, 2404, 2867, 3034, 3176
FriOI GRGCYC 3 cut(s) 891, 1850, 2722
FspBI CTAG 5 cut(s) 809, 1721, 2123, 2621, 3195
GlaI GCGC 9 cut(s) 115, 194, 325, 513, 539, 711, 1598, 2108, 3071
GsuI CTGGAG 1 cut(s) 613
HaeII RGCGCY 3 cut(s) 713, 1600, 3073
HaeIII GGCC 7 cut(s) 559, 817, 1745, 1767, 3170, 3222, 3400
HapII CCGG 4 cut(s) 285, 1256, 1961, 2336
HgaI GACGC 5 cut(s) 284, 297, 487, 838, 998
HhaI GCGC 9 cut(s) 116, 195, 326, 514, 540, 712, 1599, 2109, 3072
Hin1I GRCGYC 5 cut(s) 276, 426, 498, 710, 830
Hin6I GCGC 9 cut(s) 114, 193, 324, 512, 538, 710, 1597, 2107, 3070
HinP1I GCGC 9 cut(s) 114, 193, 324, 512, 538, 710, 1597, 2107, 3070
HincII GTYRAC 3 cut(s) 471, 502, 828
HindII GTYRAC 3 cut(s) 471, 502, 828
HindIII AAGCTT 3 cut(s) 894, 1208, 2363
HpaI GTTAAC 1 cut(s) 471
HpaII CCGG 4 cut(s) 285, 1256, 1961, 2336
HpyCH4IV ACGT 4 cut(s) 216, 362, 426, 3371
HpySE526I ACGT 4 cut(s) 216, 362, 426, 3371
Hsp92I GRCGYC 5 cut(s) 276, 426, 498, 710, 830
HspAI GCGC 9 cut(s) 114, 193, 324, 512, 538, 710, 1597, 2107, 3070
KasI GGCGCC 1 cut(s) 709
Kpn2I TCCGGA 1 cut(s) 284
Ksp22I TGATCA 4 cut(s) 477, 2326, 2653, 3391
KspAI GTTAAC 1 cut(s) 471
Lsp1109I GCAGC 4 cut(s) 450, 1020, 1063, 2591
LweI GCATC 9 cut(s) 891, 1824, 1824, 1846, 2205, 2382, 2521, 3053, 3238
MabI ACCWGGT 1 cut(s) 2899
MaeI CTAG 5 cut(s) 809, 1721, 2123, 2621, 3195
MaeII ACGT 4 cut(s) 216, 362, 426, 3371
MaeIII GTNAC 9 cut(s) 33, 42, 394, 1919, 2183, 2350, 2725, 2995, 3115
MbiI CCGCTC 3 cut(s) 140, 626, 741
MfeI CAATTG 2 cut(s) 2277, 2778
MflI RGATCY 1 cut(s) 1932
MhlI GDGCHC 4 cut(s) 891, 1850, 2722, 2929
MluI ACGCGT 1 cut(s) 990
Mly113I GGCGCC 1 cut(s) 710
MlyI GAGTC 4 cut(s) 92, 610, 1460, 2038
MmeI TCCRAC 3 cut(s) 267, 1494, 1502
Mph1103I ATGCAT 3 cut(s) 1839, 2397, 2408
MroI TCCGGA 1 cut(s) 284
MseI TTAA 5 cut(s) 56, 89, 470, 2378, 2436
MslI CAYNNNNRTG 2 cut(s) 1348, 2511
MspI CCGG 4 cut(s) 285, 1256, 1961, 2336
MspR9I CCNGG 6 cut(s) 122, 1962, 2181, 2430, 2760, 2901
MunI CAATTG 2 cut(s) 2277, 2778
Mva1269I GAATGC 3 cut(s) 1343, 2388, 2406
MvaI CCWGG 5 cut(s) 122, 2181, 2430, 2760, 2901
MvnI CGCG 8 cut(s) 195, 324, 514, 540, 992, 1363, 2109, 2964
NarI GGCGCC 1 cut(s) 710
NciI CCSGG 1 cut(s) 1962
NdeI CATATG 2 cut(s) 2092, 2424
NmeAIII GCCGAG 1 cut(s) 535
NmuCI GTSAC 6 cut(s) 33, 42, 394, 1919, 2350, 2995
NsiI ATGCAT 3 cut(s) 1839, 2397, 2408
NspI RCATGY 2 cut(s) 2027, 2943
NspV TTCGAA 3 cut(s) 1390, 2519, 3385
PaeR7I CTCGAG 1 cut(s) 230
PceI AGGCCT 1 cut(s) 3170
PcsI WCGNNNNNNNCGW 5 cut(s) 192, 414, 423, 426, 836
PctI GAATGC 3 cut(s) 1343, 2388, 2406
PflFI GACNNNGTC 1 cut(s) 425
PleI GAGTC 4 cut(s) 91, 610, 1460, 2038
PluTI GGCGCC 1 cut(s) 713
PpsI GAGTC 4 cut(s) 91, 610, 1460, 2038
PpuMI RGGWCCY 1 cut(s) 1978
Psp124BI GAGCTC 3 cut(s) 891, 1850, 2722
Psp1406I AACGTT 1 cut(s) 3371
Psp5II RGGWCCY 1 cut(s) 1978
Psp6I CCWGG 5 cut(s) 120, 2179, 2428, 2758, 2899
PspGI CCWGG 5 cut(s) 120, 2179, 2428, 2758, 2899
PspPPI RGGWCCY 1 cut(s) 1978
PspXI VCTCGAGB 1 cut(s) 230
PsrI GAACNNNNNNTAC 2 cut(s) 742, 774
PstI CTGCAG 1 cut(s) 2581
PsuI RGATCY 1 cut(s) 1932
PsyI GACNNNGTC 1 cut(s) 425
RsaI GTAC 4 cut(s) 1369, 2871, 3013, 3378
RsaNI GTAC 4 cut(s) 1368, 2870, 3012, 3377
RseI CAYNNNNRTG 2 cut(s) 1348, 2511
SacI GAGCTC 3 cut(s) 891, 1850, 2722
SalI GTCGAC 1 cut(s) 500
SaqAI TTAA 5 cut(s) 56, 89, 470, 2378, 2436
ScaI AGTACT 1 cut(s) 1369
SchI GAGTC 4 cut(s) 92, 610, 1460, 2038
ScrFI CCNGG 6 cut(s) 122, 1962, 2181, 2430, 2760, 2901
SduI GDGCHC 4 cut(s) 891, 1850, 2722, 2929
SexAI ACCWGGT 1 cut(s) 2899
SfaNI GCATC 9 cut(s) 891, 1824, 1824, 1846, 2205, 2382, 2521, 3053, 3238
SfcI CTRYAG 2 cut(s) 2577, 2682
SfoI GGCGCC 1 cut(s) 711
Sfr274I CTCGAG 1 cut(s) 230
SfuI TTCGAA 3 cut(s) 1390, 2519, 3385
SinI GGWCC 8 cut(s) 132, 352, 910, 1504, 1531, 1978, 2139, 2589
SlaI CTCGAG 1 cut(s) 230
SmiMI CAYNNNNRTG 2 cut(s) 1348, 2511
SmlI CTYRAG 8 cut(s) 206, 230, 371, 905, 2066, 2102, 2271, 2858
SmoI CTYRAG 8 cut(s) 206, 230, 371, 905, 2066, 2102, 2271, 2858
SpeI ACTAGT 1 cut(s) 2122
SseBI AGGCCT 1 cut(s) 3170
SspDI GGCGCC 1 cut(s) 709
SspI AATATT 1 cut(s) 1674
SspMI CTAG 5 cut(s) 809, 1721, 2123, 2621, 3195
SstI GAGCTC 3 cut(s) 891, 1850, 2722
StuI AGGCCT 1 cut(s) 3170
StyD4I CCNGG 6 cut(s) 120, 1960, 2179, 2428, 2758, 2899
StyI CCWWGG 1 cut(s) 3326
TaiI ACGT 4 cut(s) 219, 365, 429, 3374
TaqII GACCGA 3 cut(s) 20, 3006, 3010
TatI WGTACW 1 cut(s) 1367
TauI GCSGC 6 cut(s) 114, 626, 736, 820, 878, 881
Tru1I TTAA 5 cut(s) 56, 89, 470, 2378, 2436
Tru9I TTAA 5 cut(s) 56, 89, 470, 2378, 2436
TscAI CASTG 8 cut(s) 265, 2562, 2839, 2929, 2962, 3004, 3013, 3401
TseFI GTSAC 6 cut(s) 33, 42, 394, 1919, 2350, 2995
TseI GCWGC 4 cut(s) 463, 1033, 1051, 2579
Tsp45I GTSAC 6 cut(s) 33, 42, 394, 1919, 2350, 2995
TspDTI ATGAA 5 cut(s) 1729, 2079, 2538, 3203, 3218
TspGWI ACGGA 4 cut(s) 21, 391, 2216, 2369
TspRI CASTG 8 cut(s) 265, 2562, 2839, 2929, 2962, 3004, 3013, 3401
Tth111I GACNNNGTC 1 cut(s) 425
VpaK11BI GGWCC 8 cut(s) 132, 352, 910, 1504, 1531, 1978, 2139, 2589
XagI CCTNNNNNAGG 1 cut(s) 1604
XapI RAATTY 2 cut(s) 2564, 3381
XceI RCATGY 2 cut(s) 2027, 2943
XcmI CCANNNNNNNNNTGG 2 cut(s) 1150, 1459
XhoI CTCGAG 1 cut(s) 230
XmiI GTMKAC 2 cut(s) 501, 3413
XspI CTAG 5 cut(s) 809, 1721, 2123, 2621, 3195
ZraI GACGTC 1 cut(s) 427
ZrmI AGTACT 1 cut(s) 1369
Zsp2I ATGCAT 3 cut(s) 1839, 2397, 2408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.