pycom01g13270

Adaptor-related protein complex 5, beta 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0011344
Physical Location & Seq
Forward (+)
13764837 .. 13767485
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g13270.3

Sequence Viewer

Length: 2553 bp
ATGTCCGATAAGCCACCAGAAGCACCATCTCCGTCACCGCCGTCACCTCCGCCTTTAAAGCAACTCTCTCTCCACGACTGGGAGTCGCTAATTGACGACTTCCAGCACGGCGGCGCACGCCAGCACAGATGGACATCCGCTCAGCCGATACTCCTCTCCCTCCTTGACCAAGCCCTCTCATCCATCGCCAAGCGCGAATTCCCCCTCAAGCTCCACGTCATCACCTTCCTCGAGGAGTTCTCCGACCCTCTCTTTGCCACCGCCATCGCCAACGACGCCATTTCCGGAAGCAAAGTCCTCCACCGCCTCATCGAAACCCTTCGCGCTCTGATCCGGACACCGGCCGATGGGGTCCACATCACGTTCGCTCTCAAGGAGCAGATGATGCTCTCCGTCACATCCGTCGTCGTCTCTCTCGATGACGACGTGGTGCCGATCTCCACCGTCGAATCGCTGGTGGAGCTGCTTTTAACGGTGATCAACCGTCCCAATCATGGCGTCGACCGTCAGGCGCGTGCGCTGGCGTGCGAGTGCCTTCGAGAGGTGGAGAAAGAGAGCTTGGGTGGTTTGAATTACAAGGAGTTGTTGAGGGCGATGGGTTTTTTGCTGGAATGGCCGCAAGTGTGGACGCCGTGTGTGATGGTTGAGTTCTTGGCTCTGATAATGCCAATGGCGGCAGCATTGGAGCTCCAAATTTCGAAGCTCAAGCTGCAATTTTTCGGGATGCTTTATTCGTCTGATCTCATGCTCTGCCATATCGTTTTGACCATGTTTCCAAGGTTTTGGGACGCATTTGATGGGCAAGAAGGCAACATTGCGAGGCGGCTTGTGCTGCTGTCCAAAGAATCGCAGCACCACTTGGTTTTTCGATTGCTGGCGGTGCATTGGTTGCTGGGATTTGGTCAGTTGGTGTTGAGAAGGGAAGTAAAGAAAGTGAAGACCATTGTTGATATGGGTTCAAGATTCTATCCGAGTGTGTTCGACCCCCTTGCTTTGAAAGCCTTGAAGCTTGACCTGCTTGCATTTTGCTTCGTTTGTGTTGATGTCTTGAAACCAGAGAAGAGTGTTTTGGGCGAGGATGGTGAGGTGAATGATAAGTTGGTGGTGAGTATTTCACCATCTTCACATTCCAGTTTCAATGTCCAGGCTCCTCACTTTTGTCAGAGTCTCAAGAAGTCTAAGACCATCTCCTCATATGTCCACCTTGAGCGAGTAATCCCCCTACTAGTCAAACAGTCTTGGTCCTTGTCTTTATCAACTCTGGGTATTGGAAGTAACGATCCTGGTTACTTAGAAGGCATCAGGGACATTGAACCCATAGTAGAGGATAGTGATATTGATGACAGTAGTAATGTCCAGATTACACCAGAAGCTCAAAGAATTGATGACAATGGTAATGTCCAAATTATCCCTGAAGATCGAAGAATTGATCGATCACCAGAGCCATTGCGTGTGACAGATTCAAATATTTCAGAGATATTAAGAACATTGAGGACGCATTTCTCATGCATTCCTGATTTTAGACATATGCCAGGAATTAAGGTTAGACTATCATGTAGTTTGAGGTTTGAATCTGAGCCTTTCAATCGAATATGGGGAGTTGATTCCCCTGCCTGTTGTTCGAATGAATTAGATACCCTTCCTGCTCTATATGCAACAGTGCTAAAATTTTCGTCCTCTGCAGCATACGGGTCCATTTCATCATATCATATACCTTTTCTTCTAGGTGAGCCTCCCGGTAAAACAAATATACCTGATCAGACCGCCTCATTAGCTATTGTTCCTGTAGAAAATGGTTGTGGGAAAGAGGAAAGTTATAGAGCTCGTGTAACGATTGAATTGGAACCATGGGAACCCACGCCAGGTCTGATTGATGTTTCAATTGAGACAAGTGCTGAAAATGGTCAGATAATCAGGGGACAGCTTCACACTATCACTGTGGGCATAGAGGATGTGTTTCTCAAGGCTATTGTACCACCCGGCATCCAGAAAGATTCCGTACCTGGTTATTACTTAGATTTGTTCAGTGCTCTTTGGGTGGCATGTGGCACTTCCAACACTGCGCGAGAGGCGTTTCAGTTGAAAGGAGGCAAAGGGGTTACCGCTATAAGCGGTACTGGGTCAGTCAAGCTACTAGAAGTCCCTGCATCGTCTTTGATTCAGGCTACTGAGCGCTATTTGGCACCCTTCGTTGTAAGTGTGATAGGTGAACCACTTGGTACTGTCGTAAAGGATGGAGGAATCATTAGGGACATCATCTGGAAGGACGAGGCCTCAGATTCTTCCCTAGACATTACTAGCTCGGAGACCAATTTTGATAGAGGCCCGCTTCATCTTACATTCGGTGACGATGCAGATGAAAGGGACAGTCATGTCAACACCCGCAAAAGAAACATGGGTAGCATACTCATTTTGATATTTCTTCCACCAAGGTATCATCTCCTCTTCCAAATGGAAGTGTCCGATGTTTCATCGTTAGTACGGATTCAAACTGATCACTGGCCGTGCTTAGCTTATACTGATGATTATTTGGAAGCTTTATTTTTGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

851

Amino Acids

94.27

Weight (kDa)

5.25

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19870 AT3G19870
fragaria_vesca FvH4_7g19060
malus_domestica MD01G1105900.v1.1 MD07G1172300.v1.1
prunus_persica Prupe.2G211300_v2.0.a1
pyrus_communis pycom01g13270 pycom07g16670 pycom07g16680
rosa_chinensis RchiOBHm_Chr1g0362961
rosa_laevigata RLG00000027607
rosa_multiflora Rmu_sc0000174.1_g000010
rosa_roxburghii Rroxscaffold_4G00293600
rosa_rugosa Rorug01G0302400
rosa_samantha Rh1AG311900 Rh1BG275100 Rh1CG292200 Rh1DG305800 Rh7CG217100
rosa_wichuraiana Rw1G027650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2372
AbsI CCTCGAGG 1 cut(s) 230
Acc36I ACCTGC 1 cut(s) 1023
AccB1I GGYRCC 2 cut(s) 430, 2182
AccBSI CCGCTC 1 cut(s) 140
AccI GTMKAC 1 cut(s) 501
AccII CGCG 4 cut(s) 195, 324, 514, 2065
AccIII TCCGGA 2 cut(s) 284, 333
AclWI GGATC 2 cut(s) 325, 1274
AcoI YGGCCR 3 cut(s) 342, 614, 2501
AcsI RAATTY 3 cut(s) 197, 693, 1667
AcuI CTGAAG 1 cut(s) 1434
AcyI GRCGYC 3 cut(s) 276, 498, 629
AfaI GTAC 5 cut(s) 1974, 2001, 2116, 2221, 2481
AfeI AGCGCT 1 cut(s) 2174
AfiI CCNNNNNNNGG 6 cut(s) 79, 340, 347, 494, 541, 1862
AhdI GACNNNNNGTC 1 cut(s) 82
AhlI ACTAGT 1 cut(s) 1225
AjiI CACGTC 2 cut(s) 217, 427
AjnI CCWGG 5 cut(s) 1143, 1282, 1531, 1861, 2002
AjuI GAANNNNNNNTTGG 8 cut(s) 182, 214, 542, 574, 1052, 1082, 1084, 1114
Alw21I GWGCWC 3 cut(s) 690, 1825, 2032
Alw26I GTCTC 4 cut(s) 415, 1172, 1880, 2300
AlwI GGATC 2 cut(s) 325, 1274
Ama87I CYCGRG 1 cut(s) 230
Aor13HI TCCGGA 2 cut(s) 284, 333
Aor51HI AGCGCT 1 cut(s) 2174
AoxI GGCC 5 cut(s) 342, 614, 2271, 2323, 2501
ApeKI GCWGC 6 cut(s) 463, 677, 709, 832, 850, 1682
ApoI RAATTY 3 cut(s) 197, 693, 1667
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
Asp700I GAANNNNTTC 1 cut(s) 318
AspLEI GCGC 7 cut(s) 116, 195, 326, 514, 520, 2065, 2175
AspS9I GGNCC 4 cut(s) 352, 1242, 1692, 2324
AsuC2I CCSGG 2 cut(s) 1737, 1980
AsuII TTCGAA 2 cut(s) 698, 1622
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 3 cut(s) 352, 1242, 1692
BaeI ACNNNNGTAYC 4 cut(s) 2211, 2244, 2417, 2450
BanI GGYRCC 2 cut(s) 430, 2182
BanII GRGCYC 2 cut(s) 690, 1825
BauI CACGAG 1 cut(s) 1824
BbsI GAAGAC 1 cut(s) 944
Bbv12I GWGCWC 3 cut(s) 690, 1825, 2032
BbvI GCAGC 6 cut(s) 450, 689, 696, 819, 862, 1694
BceAI ACGGC 4 cut(s) 25, 124, 616, 2488
BcgI CGANNNNNNTGC 2 cut(s) 971, 1005
BciT130I CCWGG 5 cut(s) 1145, 1284, 1533, 1863, 2004
BclI TGATCA 3 cut(s) 477, 1756, 2494
BcnI CCSGG 2 cut(s) 1737, 1980
BcoDI GTCTC 4 cut(s) 415, 1172, 1880, 2300
BcuI ACTAGT 1 cut(s) 1225
BfaI CTAG 5 cut(s) 1226, 1724, 2135, 2288, 2298
BfmI CTRYAG 2 cut(s) 1680, 1785
BfoI RGCGCY 1 cut(s) 2176
BfuAI ACCTGC 1 cut(s) 1023
BlpI GCTNAGC 2 cut(s) 141, 2509
Bme1390I CCNGG 7 cut(s) 1145, 1284, 1533, 1737, 1863, 1980, 2004
Bme18I GGWCC 3 cut(s) 352, 1242, 1692
BmeRI GACNNNNNGTC 1 cut(s) 82
BmeT110I CYCGRG 1 cut(s) 230
BmgBI CACGTC 2 cut(s) 217, 427
BmgT120I GGNCC 4 cut(s) 352, 1242, 1692, 2324
BmiI GGNNCC 7 cut(s) 353, 432, 1149, 1693, 1845, 1854, 2184
BmrFI CCNGG 7 cut(s) 1145, 1284, 1533, 1737, 1863, 1980, 2004
BmrI ACTGGG 2 cut(s) 88, 2127
BmsI GCATC 6 cut(s) 375, 714, 1308, 1992, 2156, 2341
BmuI ACTGGG 2 cut(s) 88, 2127
BpiI GAAGAC 1 cut(s) 944
BplI GAGNNNNNCTC 2 cut(s) 224, 256
Bpu1102I GCTNAGC 2 cut(s) 141, 2509
Bpu14I TTCGAA 2 cut(s) 698, 1622
BpuEI CTTGAG 6 cut(s) 191, 356, 689, 1154, 1226, 1946
BpuMI CCSGG 2 cut(s) 1737, 1980
Bsa29I ATCGAT 1 cut(s) 1432
BsaBI GATNNNNATC 1 cut(s) 133
BsaHI GRCGYC 3 cut(s) 276, 498, 629
BsaI GGTCTC 1 cut(s) 2300
BsaJI CCNNGG 3 cut(s) 776, 1847, 2429
BsaWI WCCGGW 2 cut(s) 284, 333
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 6 cut(s) 79, 340, 347, 494, 541, 1862
Bse118I RCCGGY 1 cut(s) 340
Bse1I ACTGG 4 cut(s) 83, 1131, 2122, 2504
Bse3DI GCAATG 2 cut(s) 813, 1445
Bse8I GATNNNNATC 1 cut(s) 133
BseAI TCCGGA 2 cut(s) 284, 333
BseBI CCWGG 5 cut(s) 1145, 1284, 1533, 1863, 2004
BseCI ATCGAT 1 cut(s) 1432
BseDI CCNNGG 3 cut(s) 776, 1847, 2429
BseGI GGATG 8 cut(s) 134, 179, 398, 729, 1084, 1957, 1983, 2239
BseJI GATNNNNATC 1 cut(s) 133
BseLI CCNNNNNNNGG 6 cut(s) 79, 340, 347, 494, 541, 1862
BseMI GCAATG 2 cut(s) 813, 1445
BseMII CTCAG 4 cut(s) 155, 1566, 2160, 2289
BseNI ACTGG 4 cut(s) 83, 1131, 2122, 2504
BseRI GAGGAG 5 cut(s) 143, 248, 1140, 1180, 2432
BseX3I CGGCCG 1 cut(s) 342
BseXI GCAGC 6 cut(s) 450, 689, 696, 819, 862, 1694
BseYI CCCAGC 1 cut(s) 892
Bsh1236I CGCG 4 cut(s) 195, 324, 514, 2065
Bsh1285I CGRYCG 2 cut(s) 345, 505
BshFI GGCC 5 cut(s) 344, 616, 2273, 2325, 2503
BshNI GGYRCC 2 cut(s) 430, 2182
BshVI ATCGAT 1 cut(s) 1432
BsiEI CGRYCG 2 cut(s) 345, 505
BsiHKAI GWGCWC 3 cut(s) 690, 1825, 2032
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 5 cut(s) 285, 334, 341, 1737, 1980
BslFI GGGAC 7 cut(s) 471, 800, 1319, 1932, 2126, 2264, 2378
BslI CCNNNNNNNGG 6 cut(s) 79, 340, 347, 494, 541, 1862
BsmAI GTCTC 4 cut(s) 415, 1172, 1880, 2300
BsmBI CGTCTC 1 cut(s) 415
BsmFI GGGAC 7 cut(s) 471, 800, 1319, 1932, 2126, 2264, 2378
BsmI GAATGC 1 cut(s) 1509
BsnI GGCC 5 cut(s) 344, 616, 2273, 2325, 2503
Bso31I GGTCTC 1 cut(s) 2300
BsoBI CYCGRG 1 cut(s) 230
Bsp119I TTCGAA 2 cut(s) 698, 1622
Bsp1286I GDGCHC 3 cut(s) 690, 1825, 2032
Bsp13I TCCGGA 2 cut(s) 284, 333
Bsp1720I GCTNAGC 2 cut(s) 141, 2509
Bsp19I CCATGG 1 cut(s) 1847
BspANI GGCC 5 cut(s) 344, 616, 2273, 2325, 2503
BspCNI CTCAG 4 cut(s) 154, 1567, 2161, 2288
BspDI ATCGAT 1 cut(s) 1432
BspEI TCCGGA 2 cut(s) 284, 333
BspFNI CGCG 4 cut(s) 195, 324, 514, 2065
BspLI GGNNCC 7 cut(s) 353, 432, 1149, 1693, 1845, 1854, 2184
BspMAI CTGCAG 1 cut(s) 1684
BspMI ACCTGC 1 cut(s) 1023
BspPI GGATC 2 cut(s) 325, 1274
BspT104I TTCGAA 2 cut(s) 698, 1622
BspT107I GGYRCC 2 cut(s) 430, 2182
BspTNI GGTCTC 1 cut(s) 2300
BsrBI CCGCTC 1 cut(s) 140
BsrDI GCAATG 2 cut(s) 813, 1445
BsrFI RCCGGY 1 cut(s) 340
BsrI ACTGG 4 cut(s) 83, 1131, 2122, 2504
BssAI RCCGGY 1 cut(s) 340
BssECI CCNNGG 3 cut(s) 776, 1847, 2429
BssNI GRCGYC 3 cut(s) 276, 498, 629
BssSI CACGAG 1 cut(s) 1824
BssT1I CCWWGG 3 cut(s) 776, 1847, 2429
Bst2BI CACGAG 1 cut(s) 1824
Bst2UI CCWGG 5 cut(s) 1145, 1284, 1533, 1863, 2004
Bst6I CTCTTC 2 cut(s) 1055, 2450
BstACI GRCGYC 3 cut(s) 276, 498, 629
BstAPI GCANNNNNTGC 2 cut(s) 385, 889
BstBI TTCGAA 2 cut(s) 698, 1622
BstC8I GCNNGC 8 cut(s) 118, 122, 516, 522, 526, 876, 1020, 2327
BstDEI CTNAG 8 cut(s) 141, 1179, 1291, 1575, 2014, 2169, 2275, 2509
BstDSI CCRYGG 1 cut(s) 1847
BstEII GGTNACC 1 cut(s) 2098
BstENI CCTNNNNNAGG 1 cut(s) 539
BstF5I GGATG 8 cut(s) 134, 179, 398, 729, 1084, 1957, 1983, 2239
BstFNI CGCG 4 cut(s) 195, 324, 514, 2065
BstH2I RGCGCY 1 cut(s) 2176
BstHHI GCGC 7 cut(s) 116, 195, 326, 514, 520, 2065, 2175
BstMAI GTCTC 4 cut(s) 415, 1172, 1880, 2300
BstMCI CGRYCG 2 cut(s) 345, 505
BstNI CCWGG 5 cut(s) 1145, 1284, 1533, 1863, 2004
BstNSI RCATGY 1 cut(s) 2046
BstPI GGTNACC 1 cut(s) 2098
BstSCI CCNGG 7 cut(s) 1143, 1282, 1531, 1735, 1861, 1978, 2002
BstSFI CTRYAG 2 cut(s) 1680, 1785
BstUI CGCG 4 cut(s) 195, 324, 514, 2065
BstV1I GCAGC 6 cut(s) 450, 689, 696, 819, 862, 1694
BstV2I GAAGAC 1 cut(s) 944
BstXI CCANNNNNNTGG 1 cut(s) 783
BstZI CGGCCG 1 cut(s) 342
Bsu15I ATCGAT 1 cut(s) 1432
BsuRI GGCC 5 cut(s) 344, 616, 2273, 2325, 2503
BsuTUI ATCGAT 1 cut(s) 1432
BtgI CCRYGG 1 cut(s) 1847
BtgZI GCGATG 3 cut(s) 169, 250, 608
BtrI CACGTC 2 cut(s) 217, 427
BtsCI GGATG 8 cut(s) 134, 179, 398, 729, 1084, 1957, 1983, 2239
BtsI GCAGTG 1 cut(s) 2058
BtsIMutI CAGTG 5 cut(s) 1665, 1935, 2032, 2058, 2497
BveI ACCTGC 1 cut(s) 1023
Cac8I GCNNGC 8 cut(s) 118, 122, 516, 522, 526, 876, 1020, 2327
CfoI GCGC 7 cut(s) 116, 195, 326, 514, 520, 2065, 2175
Cfr10I RCCGGY 1 cut(s) 340
Cfr13I GGNCC 4 cut(s) 352, 1242, 1692, 2324
ClaI ATCGAT 1 cut(s) 1432
CseI GACGC 5 cut(s) 284, 487, 637, 797, 1504
CsiI ACCWGGT 1 cut(s) 2002
Csp6I GTAC 5 cut(s) 1973, 2000, 2115, 2220, 2480
CviAII CATG 9 cut(s) 494, 745, 769, 1506, 1554, 1848, 2043, 2372, 2395
CviQI GTAC 5 cut(s) 1973, 2000, 2115, 2220, 2480
DdeI CTNAG 8 cut(s) 141, 1179, 1291, 1575, 2014, 2169, 2275, 2509
DraI TTTAAA 1 cut(s) 57
DrdI GACNNNNNNGTC 1 cut(s) 2372
DriI GACNNNNNGTC 1 cut(s) 82
DseDI GACNNNNNNGTC 1 cut(s) 2372
EaeI YGGCCR 3 cut(s) 342, 614, 2501
EagI CGGCCG 1 cut(s) 342
Eam1104I CTCTTC 2 cut(s) 1055, 2450
Eam1105I GACNNNNNGTC 1 cut(s) 82
EarI CTCTTC 2 cut(s) 1055, 2450
EciI GGCGGA 1 cut(s) 39
Ecl136II GAGCTC 2 cut(s) 688, 1823
EclXI CGGCCG 1 cut(s) 342
Eco130I CCWWGG 3 cut(s) 776, 1847, 2429
Eco147I AGGCCT 1 cut(s) 2273
Eco24I GRGCYC 2 cut(s) 690, 1825
Eco31I GGTCTC 1 cut(s) 2300
Eco47I GGWCC 3 cut(s) 352, 1242, 1692
Eco47III AGCGCT 1 cut(s) 2174
Eco52I CGGCCG 1 cut(s) 342
Eco53kI GAGCTC 2 cut(s) 688, 1823
Eco57I CTGAAG 1 cut(s) 1434
Eco88I CYCGRG 1 cut(s) 230
Eco91I GGTNACC 1 cut(s) 2098
EcoICRI GAGCTC 2 cut(s) 688, 1823
EcoNI CCTNNNNNAGG 1 cut(s) 539
EcoO65I GGTNACC 1 cut(s) 2098
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 5 cut(s) 1143, 1282, 1531, 1861, 2002
EcoT14I CCWWGG 3 cut(s) 776, 1847, 2429
EcoT22I ATGCAT 1 cut(s) 1511
EcoT38I GRGCYC 2 cut(s) 690, 1825
ErhI CCWWGG 3 cut(s) 776, 1847, 2429
Esp3I CGTCTC 1 cut(s) 415
FaeI CATG 9 cut(s) 497, 748, 772, 1509, 1557, 1851, 2046, 2375, 2398
FaqI GGGAC 7 cut(s) 471, 800, 1319, 1932, 2126, 2264, 2378
FatI CATG 9 cut(s) 493, 744, 768, 1505, 1553, 1847, 2042, 2371, 2394
FauI CCCGC 2 cut(s) 2334, 2390
FauNDI CATATG 2 cut(s) 1195, 1527
FbaI TGATCA 3 cut(s) 477, 1756, 2494
FblI GTMKAC 1 cut(s) 501
FokI GGATG 8 cut(s) 121, 166, 385, 736, 1091, 1964, 1970, 2246
FriOI GRGCYC 2 cut(s) 690, 1825
FspBI CTAG 5 cut(s) 1226, 1724, 2135, 2288, 2298
GlaI GCGC 7 cut(s) 115, 194, 325, 513, 519, 2064, 2174
GsaI CCCAGC 1 cut(s) 896
HaeII RGCGCY 1 cut(s) 2176
HaeIII GGCC 5 cut(s) 344, 616, 2273, 2325, 2503
HapII CCGG 5 cut(s) 285, 334, 341, 1737, 1980
HgaI GACGC 5 cut(s) 284, 487, 637, 797, 1504
HhaI GCGC 7 cut(s) 116, 195, 326, 514, 520, 2065, 2175
Hin1I GRCGYC 3 cut(s) 276, 498, 629
Hin1II CATG 9 cut(s) 497, 748, 772, 1509, 1557, 1851, 2046, 2375, 2398
Hin6I GCGC 7 cut(s) 114, 193, 324, 512, 518, 2063, 2173
HinP1I GCGC 7 cut(s) 114, 193, 324, 512, 518, 2063, 2173
HincII GTYRAC 2 cut(s) 502, 2377
HindII GTYRAC 2 cut(s) 502, 2377
HindIII AAGCTT 2 cut(s) 1007, 2535
HpaII CCGG 5 cut(s) 285, 334, 341, 1737, 1980
Hpy166II GTNNAC 6 cut(s) 355, 502, 627, 1201, 2210, 2377
Hpy8I GTNNAC 6 cut(s) 355, 502, 627, 1201, 2210, 2377
Hpy99I CGWCG 6 cut(s) 278, 407, 410, 428, 449, 503
HpyAV CCTTC 9 cut(s) 235, 329, 545, 800, 912, 1289, 1649, 2197, 2257
HpyCH4IV ACGT 3 cut(s) 216, 362, 426
HpyCH4V TGCA 8 cut(s) 712, 883, 1022, 1509, 1655, 1682, 2147, 2354
HpyF3I CTNAG 8 cut(s) 141, 1179, 1291, 1575, 2014, 2169, 2275, 2509
HpySE526I ACGT 3 cut(s) 216, 362, 426
Hsp92I GRCGYC 3 cut(s) 276, 498, 629
Hsp92II CATG 9 cut(s) 497, 748, 772, 1509, 1557, 1851, 2046, 2375, 2398
HspAI GCGC 7 cut(s) 114, 193, 324, 512, 518, 2063, 2173
Kpn2I TCCGGA 2 cut(s) 284, 333
Ksp22I TGATCA 3 cut(s) 477, 1756, 2494
LmnI GCTCC 6 cut(s) 216, 376, 460, 685, 693, 1153
Lsp1109I GCAGC 6 cut(s) 450, 689, 696, 819, 862, 1694
LweI GCATC 6 cut(s) 375, 714, 1308, 1992, 2156, 2341
MabI ACCWGGT 1 cut(s) 2002
MaeI CTAG 5 cut(s) 1226, 1724, 2135, 2288, 2298
MaeII ACGT 3 cut(s) 216, 362, 426
MaeIII GTNAC 9 cut(s) 33, 42, 394, 1274, 1286, 1453, 1828, 2098, 2345
MbiI CCGCTC 1 cut(s) 140
MboII GAAGA 9 cut(s) 949, 1072, 1113, 1427, 1434, 1712, 2274, 2414, 2437
MfeI CAATTG 1 cut(s) 1881
MhlI GDGCHC 3 cut(s) 690, 1825, 2032
MlyI GAGTC 2 cut(s) 92, 1174
MmeI TCCRAC 2 cut(s) 267, 2079
Mph1103I ATGCAT 1 cut(s) 1511
MroI TCCGGA 2 cut(s) 284, 333
MroXI GAANNNNTTC 1 cut(s) 318
MseI TTAA 4 cut(s) 56, 470, 1481, 1539
MspI CCGG 5 cut(s) 285, 334, 341, 1737, 1980
MspR9I CCNGG 7 cut(s) 1145, 1284, 1533, 1737, 1863, 1980, 2004
MunI CAATTG 1 cut(s) 1881
Mva1269I GAATGC 1 cut(s) 1509
MvaI CCWGG 5 cut(s) 1145, 1284, 1533, 1863, 2004
MvnI CGCG 4 cut(s) 195, 324, 514, 2065
NciI CCSGG 2 cut(s) 1737, 1980
NcoI CCATGG 1 cut(s) 1847
NdeI CATATG 2 cut(s) 1195, 1527
NlaIII CATG 9 cut(s) 497, 748, 772, 1509, 1557, 1851, 2046, 2375, 2398
NlaIV GGNNCC 7 cut(s) 353, 432, 1149, 1693, 1845, 1854, 2184
NmuCI GTSAC 5 cut(s) 33, 42, 394, 1453, 2345
NsiI ATGCAT 1 cut(s) 1511
NspI RCATGY 1 cut(s) 2046
NspV TTCGAA 2 cut(s) 698, 1622
PaeR7I CTCGAG 1 cut(s) 230
PceI AGGCCT 1 cut(s) 2273
PcsI WCGNNNNNNNCGW 3 cut(s) 192, 414, 423
PctI GAATGC 1 cut(s) 1509
PdmI GAANNNNTTC 1 cut(s) 318
PleI GAGTC 2 cut(s) 91, 1173
PpsI GAGTC 2 cut(s) 91, 1173
Psp124BI GAGCTC 2 cut(s) 690, 1825
Psp6I CCWGG 5 cut(s) 1143, 1282, 1531, 1861, 2002
PspEI GGTNACC 1 cut(s) 2098
PspFI CCCAGC 1 cut(s) 892
PspGI CCWGG 5 cut(s) 1143, 1282, 1531, 1861, 2002
PspN4I GGNNCC 7 cut(s) 353, 432, 1149, 1693, 1845, 1854, 2184
PspPI GGNCC 4 cut(s) 352, 1242, 1692, 2324
PspXI VCTCGAGB 1 cut(s) 230
PstI CTGCAG 1 cut(s) 1684
RsaI GTAC 5 cut(s) 1974, 2001, 2116, 2221, 2481
RsaNI GTAC 5 cut(s) 1973, 2000, 2115, 2220, 2480
SacI GAGCTC 2 cut(s) 690, 1825
SalI GTCGAC 1 cut(s) 500
SaqAI TTAA 4 cut(s) 56, 470, 1481, 1539
Sau96I GGNCC 4 cut(s) 352, 1242, 1692, 2324
SchI GAGTC 2 cut(s) 92, 1174
ScrFI CCNGG 7 cut(s) 1145, 1284, 1533, 1737, 1863, 1980, 2004
SduI GDGCHC 3 cut(s) 690, 1825, 2032
SexAI ACCWGGT 1 cut(s) 2002
SfaNI GCATC 6 cut(s) 375, 714, 1308, 1992, 2156, 2341
SfcI CTRYAG 2 cut(s) 1680, 1785
Sfr274I CTCGAG 1 cut(s) 230
SfuI TTCGAA 2 cut(s) 698, 1622
SinI GGWCC 3 cut(s) 352, 1242, 1692
SlaI CTCGAG 1 cut(s) 230
SmlI CTYRAG 7 cut(s) 206, 230, 371, 704, 1169, 1205, 1961
SmoI CTYRAG 7 cut(s) 206, 230, 371, 704, 1169, 1205, 1961
SpeI ACTAGT 1 cut(s) 1225
SseBI AGGCCT 1 cut(s) 2273
SspI AATATT 1 cut(s) 1468
SspMI CTAG 5 cut(s) 1226, 1724, 2135, 2288, 2298
SstI GAGCTC 2 cut(s) 690, 1825
StuI AGGCCT 1 cut(s) 2273
StyD4I CCNGG 7 cut(s) 1143, 1282, 1531, 1735, 1861, 1978, 2002
StyI CCWWGG 3 cut(s) 776, 1847, 2429
TaiI ACGT 3 cut(s) 219, 365, 429
TauI GCSGC 4 cut(s) 114, 619, 677, 826
Tru1I TTAA 4 cut(s) 56, 470, 1481, 1539
Tru9I TTAA 4 cut(s) 56, 470, 1481, 1539
TscAI CASTG 5 cut(s) 1665, 1942, 2032, 2065, 2504
TseFI GTSAC 5 cut(s) 33, 42, 394, 1453, 2345
TseI GCWGC 6 cut(s) 463, 677, 709, 832, 850, 1682
Tsp45I GTSAC 5 cut(s) 33, 42, 394, 1453, 2345
TspDTI ATGAA 5 cut(s) 1641, 1689, 2321, 2373, 2460
TspGWI ACGGA 5 cut(s) 21, 382, 391, 1987, 2497
TspRI CASTG 5 cut(s) 1665, 1942, 2032, 2065, 2504
VpaK11BI GGWCC 3 cut(s) 352, 1242, 1692
XagI CCTNNNNNAGG 1 cut(s) 539
XapI RAATTY 3 cut(s) 197, 693, 1667
XceI RCATGY 1 cut(s) 2046
XcmI CCANNNNNNNNNTGG 1 cut(s) 949
XhoI CTCGAG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 501
XmnI GAANNNNTTC 1 cut(s) 318
XspI CTAG 5 cut(s) 1226, 1724, 2135, 2288, 2298
Zsp2I ATGCAT 1 cut(s) 1511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.