Rorug01G0302400

Adaptor-related protein complex 5, beta 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0011344
Physical Location & Seq
Forward (+)
41830113 .. 41831340
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0302400.1

Sequence Viewer

Length: 894 bp
ATGGATTCAAACTACCCAACCAAGTACACAGAGAAGAGAACCCTCACCAAGAAGCTTGTCAAGCATTTCCCGAACGTTGCAGTCCCTAAGATTGTCCGGATTTCCGTCACCGACGAGGACGCGACCGACACGTCCAGCGACGAAGAAGAACAAGACCAACAACAACGACTGACGAAGAGGGTCATCAACGAAGTGAGAATCGAAGAGGAGTCCGAGGCGGTCAGGGTGTTTCTGGCCAAGAATCAGAAGCAAAAGCCGAAGCCTTTACCGGAGGAGAAGAGGAAGTTCCGGGGCGTACGGCAGCGGCCGTGGGGCAAATGGGCCGCCGAGATTCGTGACCCGGTCCGCAAGTCCCGTGTCTGGCTCGGAACGTTCCAGACGGCGGAGGAGGCGGCGATGGTGTACGACAGAGCTGCCATTCAGTTGAGGGGACATAACGCTCTCACGAATATCATCGCTCCTCCTTCCAAAATTCTCATTCCGGATCACGATTCTTCTTCGAATGAGTCCAGTTCGATCAACAATCTTTCTTGTTCGAATGCGTCCAGTTCGATCGACCATATTGTTGTGATAGATGAGTATGATTCCGGGAAAGAGTGTCAGAGCATTCGCTCCCCGACCTCTGTTCTCAGTGGTAGTGACAACGAGTGGAGACCTGGGTCGAAGGAGCAGGATTTCGTTAAAGACTATGGTTACTTTGATGGTGTGATCAGTCCCACTGAAGCTATATTCTTCGGTGTTACAGAGCCGATTCTGTTGGAGGAAATGAACTTGGTGCCGGAAACAATGTTGCGAGACGATTTGTCCGACATTTCGCTGGATTCCGTTGGGGATTTCAGGTCATGTAAATGGAATGTGGAGGATTTCTTTCAAGATCAATGTGGTGGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

297

Amino Acids

33.72

Weight (kDa)

5.05

Isoelectric Point (pI)

61.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 95 - 145 3.6e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19870 AT3G19870
fragaria_vesca FvH4_7g19060
malus_domestica MD01G1105900.v1.1 MD07G1172300.v1.1
prunus_persica Prupe.2G211300_v2.0.a1
pyrus_communis pycom01g13270 pycom07g16670 pycom07g16680
rosa_chinensis RchiOBHm_Chr1g0362961
rosa_laevigata RLG00000027607
rosa_multiflora Rmu_sc0000174.1_g000010
rosa_roxburghii Rroxscaffold_4G00293600
rosa_rugosa Rorug01G0302400
rosa_samantha Rh1AG311900 Rh1BG275100 Rh1CG292200 Rh1DG305800 Rh7CG217100
rosa_wichuraiana Rw1G027650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 775
AccII CGCG 1 cut(s) 122
AccIII TCCGGA 2 cut(s) 96, 481
AciI CCGC 6 cut(s) 218, 304, 324, 346, 383, 392
AclI AACGTT 2 cut(s) 75, 371
AclWI GGATC 1 cut(s) 492
AcoI YGGCCR 2 cut(s) 234, 305
AcsI RAATTY 1 cut(s) 471
AcuI CTGAAG 1 cut(s) 741
AfaI GTAC 3 cut(s) 26, 297, 404
AfiI CCNNNNNNNGG 2 cut(s) 360, 382
AflIII ACRYGT 1 cut(s) 129
AgsI TTSAA 2 cut(s) 9, 872
AhdI GACNNNNNGTC 1 cut(s) 802
AjiI CACGTC 1 cut(s) 132
AjnI CCWGG 1 cut(s) 655
AluBI AGCT 3 cut(s) 55, 413, 725
AluI AGCT 3 cut(s) 55, 413, 725
Alw26I GTCTC 2 cut(s) 646, 789
AlwI GGATC 1 cut(s) 492
Aor13HI TCCGGA 2 cut(s) 96, 481
AoxI GGCC 3 cut(s) 234, 305, 321
ApeKI GCWGC 2 cut(s) 301, 413
ApoI RAATTY 1 cut(s) 471
AspS9I GGNCC 2 cut(s) 321, 343
AsuC2I CCSGG 3 cut(s) 290, 341, 589
AsuHPI GGTGA 2 cut(s) 37, 100
AsuII TTCGAA 2 cut(s) 500, 536
AvaII GGWCC 1 cut(s) 343
BalI TGGCCA 1 cut(s) 236
BanI GGYRCC 1 cut(s) 775
BbvI GCAGC 2 cut(s) 313, 400
BccI CCATC 2 cut(s) 391, 695
BceAI ACGGC 3 cut(s) 292, 314, 396
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BciT130I CCWGG 1 cut(s) 657
BclI TGATCA 1 cut(s) 708
BcnI CCSGG 3 cut(s) 290, 341, 589
BcoDI GTCTC 2 cut(s) 646, 789
BisI GCNGC 5 cut(s) 302, 305, 324, 393, 414
BlsI GCNGC 5 cut(s) 303, 306, 325, 394, 415
Bme1390I CCNGG 4 cut(s) 290, 341, 589, 657
Bme18I GGWCC 1 cut(s) 343
BmeRI GACNNNNNGTC 1 cut(s) 802
BmgBI CACGTC 1 cut(s) 132
BmgT120I GGNCC 2 cut(s) 321, 343
BmiI GGNNCC 1 cut(s) 777
BmrFI CCNGG 4 cut(s) 290, 341, 589, 657
BoxI GACNNNNGTC 1 cut(s) 658
Bpu14I TTCGAA 2 cut(s) 500, 536
BpuMI CCSGG 3 cut(s) 290, 341, 589
BsaI GGTCTC 1 cut(s) 646
BsaJI CCNNGG 4 cut(s) 213, 289, 308, 656
BsaWI WCCGGW 3 cut(s) 96, 268, 481
Bsc4I CCNNNNNNNGG 2 cut(s) 360, 382
Bse1I ACTGG 2 cut(s) 510, 546
BseAI TCCGGA 2 cut(s) 96, 481
BseBI CCWGG 1 cut(s) 657
BseDI CCNNGG 4 cut(s) 213, 289, 308, 656
BseLI CCNNNNNNNGG 2 cut(s) 360, 382
BseMII CTCAG 1 cut(s) 643
BseNI ACTGG 2 cut(s) 510, 546
BseRI GAGGAG 4 cut(s) 221, 287, 401, 450
BseX3I CGGCCG 1 cut(s) 305
BseXI GCAGC 2 cut(s) 313, 400
Bsh1236I CGCG 1 cut(s) 122
Bsh1285I CGRYCG 3 cut(s) 126, 308, 555
BshFI GGCC 3 cut(s) 236, 307, 323
BshNI GGYRCC 1 cut(s) 775
BsiEI CGRYCG 3 cut(s) 126, 308, 555
BsiSI CCGG 7 cut(s) 97, 269, 289, 341, 482, 588, 779
BsiWI CGTACG 1 cut(s) 295
BslFI GGGAC 4 cut(s) 68, 337, 444, 699
BslI CCNNNNNNNGG 2 cut(s) 360, 382
BsmAI GTCTC 2 cut(s) 646, 789
BsmBI CGTCTC 1 cut(s) 789
BsmFI GGGAC 4 cut(s) 68, 337, 444, 699
BsmI GAATGC 2 cut(s) 544, 606
BsnI GGCC 3 cut(s) 236, 307, 323
Bso31I GGTCTC 1 cut(s) 646
Bsp119I TTCGAA 2 cut(s) 500, 536
Bsp13I TCCGGA 2 cut(s) 96, 481
Bsp143I GATC 5 cut(s) 484, 516, 552, 708, 874
BspACI CCGC 6 cut(s) 218, 304, 324, 346, 383, 392
BspANI GGCC 3 cut(s) 236, 307, 323
BspCNI CTCAG 1 cut(s) 642
BspEI TCCGGA 2 cut(s) 96, 481
BspFNI CGCG 1 cut(s) 122
BspLI GGNNCC 1 cut(s) 777
BspPI GGATC 1 cut(s) 492
BspT104I TTCGAA 2 cut(s) 500, 536
BspT107I GGYRCC 1 cut(s) 775
BspTNI GGTCTC 1 cut(s) 646
BsrI ACTGG 2 cut(s) 510, 546
BssECI CCNNGG 4 cut(s) 213, 289, 308, 656
BssMI GATC 5 cut(s) 484, 516, 552, 708, 874
Bst2UI CCWGG 1 cut(s) 657
Bst6I CTCTTC 4 cut(s) 29, 170, 198, 272
BstBI TTCGAA 2 cut(s) 500, 536
BstDEI CTNAG 2 cut(s) 87, 629
BstDSI CCRYGG 1 cut(s) 308
BstFNI CGCG 1 cut(s) 122
BstKTI GATC 5 cut(s) 487, 519, 555, 711, 877
BstMAI GTCTC 2 cut(s) 646, 789
BstMBI GATC 5 cut(s) 484, 516, 552, 708, 874
BstMCI CGRYCG 3 cut(s) 126, 308, 555
BstMWI GCNNNNNNNGC 2 cut(s) 61, 389
BstNI CCWGG 1 cut(s) 657
BstPAI GACNNNNGTC 1 cut(s) 658
BstSCI CCNGG 4 cut(s) 288, 339, 587, 655
BstUI CGCG 1 cut(s) 122
BstV1I GCAGC 2 cut(s) 313, 400
BstZI CGGCCG 1 cut(s) 305
BsuRI GGCC 3 cut(s) 236, 307, 323
BtgI CCRYGG 1 cut(s) 308
BtgZI GCGATG 2 cut(s) 410, 439
BtrI CACGTC 1 cut(s) 132
BtsIMutI CAGTG 2 cut(s) 637, 717
Cfr13I GGNCC 2 cut(s) 321, 343
CpoI CGGWCCG 1 cut(s) 343
CseI GACGC 2 cut(s) 128, 531
Csp6I GTAC 3 cut(s) 25, 296, 403
CspI CGGWCCG 1 cut(s) 343
CviAII CATG 1 cut(s) 843
CviQI GTAC 3 cut(s) 25, 296, 403
DdeI CTNAG 2 cut(s) 87, 629
DpnI GATC 5 cut(s) 486, 518, 554, 710, 876
DpnII GATC 5 cut(s) 484, 516, 552, 708, 874
DrdI GACNNNNNNGTC 1 cut(s) 130
DriI GACNNNNNGTC 1 cut(s) 802
DseDI GACNNNNNNGTC 1 cut(s) 130
EaeI YGGCCR 2 cut(s) 234, 305
EagI CGGCCG 1 cut(s) 305
Eam1104I CTCTTC 4 cut(s) 29, 170, 198, 272
Eam1105I GACNNNNNGTC 1 cut(s) 802
EarI CTCTTC 4 cut(s) 29, 170, 198, 272
EciI GGCGGA 1 cut(s) 398
EclXI CGGCCG 1 cut(s) 305
Eco31I GGTCTC 1 cut(s) 646
Eco47I GGWCC 1 cut(s) 343
Eco52I CGGCCG 1 cut(s) 305
Eco57I CTGAAG 1 cut(s) 741
EcoRII CCWGG 1 cut(s) 655
Esp3I CGTCTC 1 cut(s) 789
FaeI CATG 1 cut(s) 846
FaiI YATR 6 cut(s) 435, 561, 582, 690, 728, 844
FaqI GGGAC 4 cut(s) 68, 337, 444, 699
FatI CATG 1 cut(s) 842
FbaI TGATCA 1 cut(s) 708
Fnu4HI GCNGC 5 cut(s) 302, 305, 324, 393, 414
Fsp4HI GCNGC 5 cut(s) 302, 305, 324, 393, 414
GluI GCNGC 5 cut(s) 302, 305, 324, 393, 414
HaeIII GGCC 3 cut(s) 236, 307, 323
HapII CCGG 7 cut(s) 97, 269, 289, 341, 482, 588, 779
HgaI GACGC 2 cut(s) 128, 531
Hin1II CATG 1 cut(s) 846
HindIII AAGCTT 1 cut(s) 53
HpaII CCGG 7 cut(s) 97, 269, 289, 341, 482, 588, 779
HphI GGTGA 2 cut(s) 37, 100
Hpy166II GTNNAC 2 cut(s) 27, 403
Hpy188I TCNGA 6 cut(s) 214, 246, 368, 603, 808, 893
Hpy188III TCNNGA 8 cut(s) 70, 97, 335, 376, 445, 482, 488, 872
Hpy8I GTNNAC 2 cut(s) 27, 403
Hpy99I CGWCG 2 cut(s) 116, 143
HpyAV CCTTC 2 cut(s) 474, 658
HpyCH4IV ACGT 3 cut(s) 75, 131, 371
HpyCH4V TGCA 1 cut(s) 80
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 389
HpyF3I CTNAG 2 cut(s) 87, 629
HpySE526I ACGT 3 cut(s) 75, 131, 371
Hsp92II CATG 1 cut(s) 846
Kpn2I TCCGGA 2 cut(s) 96, 481
Ksp22I TGATCA 1 cut(s) 708
Kzo9I GATC 5 cut(s) 484, 516, 552, 708, 874
LmnI GCTCC 3 cut(s) 463, 617, 667
Lsp1109I GCAGC 2 cut(s) 313, 400
MaeII ACGT 3 cut(s) 75, 131, 371
MaeIII GTNAC 5 cut(s) 106, 335, 638, 692, 739
MalI GATC 5 cut(s) 486, 518, 554, 710, 876
MboI GATC 5 cut(s) 484, 516, 552, 708, 874
MboII GAAGA 9 cut(s) 46, 155, 158, 187, 215, 289, 486, 489, 724
MlsI TGGCCA 1 cut(s) 236
MluCI AATT 1 cut(s) 471
MluNI TGGCCA 1 cut(s) 236
MlyI GAGTC 2 cut(s) 218, 515
MmeI TCCRAC 2 cut(s) 738, 831
Mox20I TGGCCA 1 cut(s) 236
MroI TCCGGA 2 cut(s) 96, 481
MscI TGGCCA 1 cut(s) 236
MseI TTAA 1 cut(s) 681
MslI CAYNNNNRTG 1 cut(s) 847
Msp20I TGGCCA 1 cut(s) 236
MspA1I CMGCKG 1 cut(s) 304
MspI CCGG 7 cut(s) 97, 269, 289, 341, 482, 588, 779
MspR9I CCNGG 4 cut(s) 290, 341, 589, 657
Mva1269I GAATGC 2 cut(s) 544, 606
MvaI CCWGG 1 cut(s) 657
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 2 cut(s) 61, 389
NciI CCSGG 3 cut(s) 290, 341, 589
NdeII GATC 5 cut(s) 484, 516, 552, 708, 874
NlaIII CATG 1 cut(s) 846
NlaIV GGNNCC 1 cut(s) 777
NmeAIII GCCGAG 1 cut(s) 352
NmuCI GTSAC 3 cut(s) 106, 335, 638
NspV TTCGAA 2 cut(s) 500, 536
PcsI WCGNNNNNNNCGW 1 cut(s) 804
PctI GAATGC 2 cut(s) 544, 606
PfeI GAWTC 8 cut(s) 5, 198, 241, 331, 491, 584, 751, 821
Pfl23II CGTACG 1 cut(s) 295
PflFI GACNNNGTC 1 cut(s) 341
PfoI TCCNGGA 1 cut(s) 587
PkrI GCNGC 5 cut(s) 303, 306, 325, 394, 415
Ple19I CGATCG 1 cut(s) 555
PleI GAGTC 2 cut(s) 217, 514
PpsI GAGTC 2 cut(s) 217, 514
PshAI GACNNNNGTC 1 cut(s) 658
Psp1406I AACGTT 2 cut(s) 75, 371
Psp6I CCWGG 1 cut(s) 655
PspGI CCWGG 1 cut(s) 655
PspLI CGTACG 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 777
PspPI GGNCC 2 cut(s) 321, 343
PsyI GACNNNGTC 1 cut(s) 341
PvuI CGATCG 1 cut(s) 555
RsaI GTAC 3 cut(s) 26, 297, 404
RsaNI GTAC 3 cut(s) 25, 296, 403
RseI CAYNNNNRTG 1 cut(s) 847
Rsr2I CGGWCCG 1 cut(s) 343
RsrII CGGWCCG 1 cut(s) 343
SaqAI TTAA 1 cut(s) 681
SatI GCNGC 5 cut(s) 302, 305, 324, 393, 414
Sau3AI GATC 5 cut(s) 484, 516, 552, 708, 874
Sau96I GGNCC 2 cut(s) 321, 343
SchI GAGTC 2 cut(s) 218, 515
ScrFI CCNGG 4 cut(s) 290, 341, 589, 657
SetI ASST 9 cut(s) 57, 78, 134, 374, 415, 623, 658, 727, 842
SfuI TTCGAA 2 cut(s) 500, 536
SinI GGWCC 1 cut(s) 343
SmiMI CAYNNNNRTG 1 cut(s) 847
Sse9I AATT 1 cut(s) 471
SsiI CCGC 6 cut(s) 218, 304, 324, 346, 383, 392
StyD4I CCNGG 4 cut(s) 288, 339, 587, 655
TaiI ACGT 3 cut(s) 78, 134, 374
TaqI TCGA 7 cut(s) 201, 500, 515, 536, 551, 555, 662
TaqII GACCGA 1 cut(s) 140
TasI AATT 1 cut(s) 471
TatI WGTACW 1 cut(s) 24
TauI GCSGC 3 cut(s) 307, 326, 395
TfiI GAWTC 8 cut(s) 5, 198, 241, 331, 491, 584, 751, 821
Tru1I TTAA 1 cut(s) 681
Tru9I TTAA 1 cut(s) 681
TscAI CASTG 2 cut(s) 637, 724
TseFI GTSAC 3 cut(s) 106, 335, 638
TseI GCWGC 2 cut(s) 301, 413
Tsp45I GTSAC 3 cut(s) 106, 335, 638
TspDTI ATGAA 1 cut(s) 782
TspGWI ACGGA 2 cut(s) 94, 814
TspRI CASTG 2 cut(s) 637, 724
Tth111I GACNNNGTC 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 343
XapI RAATTY 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.