Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0014557

Physical Location & Seq

Genomic Coordinates Forward (+)

32311678 .. 32317086

Gene Structure 5409 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1256400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2172 bp

ATGCTGAGTCTTCTCCGGAGGTCCGCCGGAAAGCTCCGGTACGAACCCGGTGCCATTTTCAACTATTCGGACCCTCTCACTACCCGGCGCCAATACACCATCGCAGTCCCACAAATCAAATCCGCCACCGCCACCGGTCTTTATGGCTTCGACCACTTAAAGTCCCCCAAAGGCTTTCAACGTTTCGTCGACGATGCAATCGAAAGGTCGGGAGAGCTGGTTGCATACATTGCCGGGATGCCTTCGTCGGCGGAAGTAGTTAGAGCCATGGATGAAATTTCTGACACAGTTTGCTCTGTCCTTGACTCCGCAGAGCTTTGTCGAAACAGTCATCCTGACAGGGAATTCGTTGAGGAGGCGACCAAAGCATCCATGAGAATGAGTGAATATCTACATTTTCTGAATACAAATCATACTTTGTATAATGCCGTGAAAAAAGCTGAGCAAGAGGGCCAATTGCTTACAGCCGAAGCTCAAAGGGTTGCACATTTCCTCCGCCTTGACTTTGAGAAGGGTGGAATTCATCTTTCTGATGAGAAATTGGATAGAGTGAATGAGCTGAATATAGAAATTTCCAAACTGTGCAGACAGTTCAATATAAATATTGACACTGACCAAGGTGCTGTAGATATTTTCCCAGCATCACGTATACCTCAAAATTTGCACCATCTCCTTAAACCCATCTATCGTTCCACATCTGTAATTTCAAGGGAATCGTTGAGGCCCAGGGCTAACATGAATGAAAAGGGATTTCGTATAACAACAGACCCACATACTCTATCCTGTGTATTGCAAGGGGCACAAGATGATGAGGTTAGGAAAATGGCATATATCAAAGGAAACTCTGTTCCTCGTGCAAACCTTAGTGTTCTCGATAAACTTATAGTTGCCCGTCATGAGCTAGCTCAGATAATGGGTTATAGATCTTATGCTGAATACACCTTGAAGCCTAACATGGCTTCATCACCAGAAGTTGTGATGTCTTTCTTGAAAGAGATGAGCGAGATGGTCAGACCTAGCGCTGATGAGGAGTTTAAAAAAATTAGGGAATTTAAGAGAGAAAAATGTGGTCAGCAATCTGGTAATTTAGAGCCATGGGATGAGGCATACTACACAGCAATGATGAAGTCTTCTGCCTATGACTTGGACTCTTCGGTTGTCGCATCGTATTTTTCTCTTCCACATTGTATCGAGGGTTTGAAAGTGCTGGTTGAATCATTATTTGGTGCTACGCTTCATAGCATCCCCCTAGCACCAGGTGAATCATGGCACCCAGATGTGCGTAAAATGGCACTTCATCATCCTGAAGAGGGTGATTTGGGGTATCTGTACCTTGATTTGTACTCAAGGAAGGGCAAGTATCCAGGTTGTGCTCATTTTGCAATTAAAGGTGGCCGCAGGGTTTCTGAATCAGAATATCAACTTCCTGTTGTTGCTCTTGTATGCAGTTTTTCTGCTTCACGTCATTCATCAACTGCGAGGCTAAATCATTGCGAACTGGAAACTCTGTTTCATGAGTTTGGACATGCTCTCCATTCGTTGCTTTCAAGAACGGATTACCAACACTTTTCAGGTACCAGGGTGATTTTTGATTTAGCGGAAACACCTTCAAACCTTTTTGAGTACTTTTTATGGGATTACCGAGTTTTGAAGACATTTGCCAAACACTATTCAACTGGTGAAATTATCCCTAAGAAACTTGTAGAGTCAATGCAGGGTGCCAGAAAAATGTTTGCTGCAACTGAATTGCAACGTCAGATTTTTTATGCTGCTATTGACCAAACATTCTTCGGGGAACAGCCAACCCCACCAACAAGCTCTATCGTTGCGGATTTGAAAAGACAGTATACTAGTTGGAAGCACATTGAGGACACCTACTGGGAGACCCGATTCAGTCACCTCTTGAATTATGGTGCAGGTTATTATAGCTATTTATACGCCAGATGTTTCGCCGCAACGATATGGCAGAAGCTGTGTCACGAGGATCCACTATCATTAACTACGGGAACTGCTTTAAGAACAAAGTTGTTACAGCATGGGGGAGCAAAAGATCCAGCCGATTTGTTAAACGGTCTTGTAGGAGATGGAATTTTGAGGGATTGTAACGGAGGAATAGTTCCTGATATCACCTGTCTTTGCGACGAAATGAAGCTGGGGGTGAAGGTATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004177 GO:0004222 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008238 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009987 GO:0016020 GO:0016787 GO:0019538 GO:0031976 GO:0031984 GO:0034357 GO:0034641 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M3

Protein Analysis

724

Amino Acids

81.82

Weight (kDa)

6.54

Isoelectric Point (pI)

40.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M3	PF01432	264 - 695	5.6e-117	Peptidase family M3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014557)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51540
fragaria_vesca	FvH4_7g27930 FvH4_7g27930
malus_domestica	MD07G1256400.v1.1
prunus_persica	Prupe.2G280500_v2.0.a1
pyrus_communis	pycom07g22930
rosa_chinensis	RchiOBHm_Chr1g0374891
rosa_laevigata	RLG00000026709
rosa_multiflora	Rmu_sc0003145.1_g000006 Rmu_sc0004947.1_g000033
rosa_roxburghii	Rroxscaffold_4G00283200
rosa_rugosa	Rorug01G0385600
rosa_samantha	Rh1AG397200 Rh1BG361000 Rh1CG374700 Rh1DG392000
rosa_wichuraiana	Rw1G035220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1907
Acc65I	GGTACC	1 cut(s)	1574
AccB1I	GGYRCC	5 cut(s)	50, 87, 1269, 1574, 1718
AccI	GTMKAC	3 cut(s)	189, 649, 1847
AccIII	TCCGGA	1 cut(s)	15
AclI	AACGTT	1 cut(s)	181
AclWI	GGATC	3 cut(s)	1979, 1992, 2045
AcoI	YGGCCR	1 cut(s)	1393
AcsI	RAATTY	7 cut(s)	276, 344, 519, 570, 658, 1049, 2088
AcuI	CTGAAG	1 cut(s)	1326
AcyI	GRCGYC	1 cut(s)	88
AdeI	CACNNNGTG	1 cut(s)	1259
AfaI	GTAC	5 cut(s)	41, 1331, 1343, 1576, 1625
AfeI	AGCGCT	1 cut(s)	1021
AfiI	CCNNNNNNNGG	1 cut(s)	1310
AgeI	ACCGGT	1 cut(s)	134
AhlI	ACTAGT	1 cut(s)	1850
AjiI	CACGTC	1 cut(s)	1463
AjnI	CCWGG	4 cut(s)	725, 1255, 1363, 1577
AjuI	GAANNNNNNNTTGG	2 cut(s)	1206, 1238
AloI	GAACNNNNNNTCC	4 cut(s)	831, 863, 2100, 2132
Alw21I	GWGCWC	1 cut(s)	1375
Alw26I	GTCTC	1 cut(s)	1877
AlwI	GGATC	3 cut(s)	1979, 1992, 2045
AlwNI	CAGNNNCTG	1 cut(s)	1972
Aor13HI	TCCGGA	1 cut(s)	15
Aor51HI	AGCGCT	1 cut(s)	1021
AoxI	GGCC	3 cut(s)	451, 722, 1393
ApeKI	GCWGC	2 cut(s)	1736, 1769
ApoI	RAATTY	7 cut(s)	276, 344, 519, 570, 658, 1049, 2088
ArsI	GACNNNNNNTTYG	2 cut(s)	329, 361
AsiGI	ACCGGT	1 cut(s)	134
Asp718I	GGTACC	1 cut(s)	1574
AspLEI	GCGC	2 cut(s)	90, 1022
AspS9I	GGNCC	4 cut(s)	21, 70, 451, 723
AsuC2I	CCSGG	3 cut(s)	48, 85, 235
AsuHPI	GGTGA	8 cut(s)	957, 1271, 1325, 1594, 1691, 1889, 2119, 2170
AsuNHI	GCTAGC	1 cut(s)	901
AvaII	GGWCC	2 cut(s)	21, 70
BaeGI	GKGCMC	1 cut(s)	802
BaeI	ACNNNNGTAYC	2 cut(s)	1566, 1599
BamHI	GGATCC	1 cut(s)	1984
BanI	GGYRCC	5 cut(s)	50, 87, 1269, 1574, 1718
BauI	CACGAG	2 cut(s)	852, 1979
BbsI	GAAGAC	3 cut(s)	2, 1122, 1658
Bbv12I	GWGCWC	1 cut(s)	1375
BbvI	GCAGC	2 cut(s)	1723, 1756
BccI	CCATC	5 cut(s)	107, 675, 689, 1000, 2078
BceAI	ACGGC	1 cut(s)	413
BcgI	CGANNNNNNTGC	2 cut(s)	32, 66
BciT130I	CCWGG	4 cut(s)	727, 1257, 1365, 1579
BciVI	GTATCC	1 cut(s)	1371
BcnI	CCSGG	3 cut(s)	48, 85, 235
BcoDI	GTCTC	1 cut(s)	1877
BcuI	ACTAGT	1 cut(s)	1850
BfaI	CTAG	4 cut(s)	902, 1017, 1250, 1851
BfmI	CTRYAG	1 cut(s)	624
BfoI	RGCGCY	2 cut(s)	91, 1023
BfuAI	ACCTGC	1 cut(s)	1907
BfuI	GTATCC	1 cut(s)	1371
BglII	AGATCT	1 cut(s)	923
BisI	GCNGC	4 cut(s)	1396, 1737, 1770, 1953
BlpI	GCTNAGC	1 cut(s)	441
BlsI	GCNGC	4 cut(s)	1397, 1738, 1771, 1954
BmcAI	AGTACT	1 cut(s)	1625
Bme1390I	CCNGG	7 cut(s)	48, 85, 235, 727, 1257, 1365, 1579
Bme18I	GGWCC	2 cut(s)	21, 70
BmgBI	CACGTC	1 cut(s)	1463
BmgT120I	GGNCC	4 cut(s)	21, 70, 451, 723
BmiI	GGNNCC	7 cut(s)	52, 72, 89, 1271, 1576, 1720, 1986
BmrFI	CCNGG	7 cut(s)	48, 85, 235, 727, 1257, 1365, 1579
BmrI	ACTGGG	1 cut(s)	1888
BmsI	GCATC	6 cut(s)	184, 228, 377, 650, 1172, 1251
BmtI	GCTAGC	1 cut(s)	905
BmuI	ACTGGG	1 cut(s)	1888
BpiI	GAAGAC	3 cut(s)	2, 1122, 1658
Bpu1102I	GCTNAGC	1 cut(s)	441
BpuEI	CTTGAG	1 cut(s)	1330
BpuMI	CCSGG	3 cut(s)	48, 85, 235
BsaAI	YACGTR	1 cut(s)	647
BsaHI	GRCGYC	1 cut(s)	88
BsaI	GGTCTC	1 cut(s)	1877
BsaJI	CCNNGG	6 cut(s)	267, 616, 725, 726, 1094, 1578
BsaWI	WCCGGW	3 cut(s)	15, 36, 134
BsaXI	ACNNNNNCTCC	8 cut(s)	204, 234, 477, 507, 1515, 1545, 2100, 2130
Bsc4I	CCNNNNNNNGG	1 cut(s)	1310
Bse118I	RCCGGY	1 cut(s)	134
Bse1I	ACTGG	3 cut(s)	1503, 1681, 1883
Bse3DI	GCAATG	3 cut(s)	228, 1125, 1489
BseAI	TCCGGA	1 cut(s)	15
BseBI	CCWGG	4 cut(s)	727, 1257, 1365, 1579
BseDI	CCNNGG	6 cut(s)	267, 616, 725, 726, 1094, 1578
BseGI	GGATG	7 cut(s)	243, 277, 331, 368, 1105, 1242, 1300
BseLI	CCNNNNNNNGG	1 cut(s)	1310
BseMI	GCAATG	3 cut(s)	228, 1125, 1489
BseMII	CTCAG	2 cut(s)	432, 920
BseNI	ACTGG	3 cut(s)	1503, 1681, 1883
BseRI	GAGGAG	2 cut(s)	368, 1043
BseSI	GKGCMC	1 cut(s)	802
BseXI	GCAGC	2 cut(s)	1723, 1756
BseYI	CCCAGC	2 cut(s)	637, 2152
BsgI	GTGCAG	2 cut(s)	604, 1935
BshFI	GGCC	3 cut(s)	453, 724, 1395
BshNI	GGYRCC	5 cut(s)	50, 87, 1269, 1574, 1718
BshTI	ACCGGT	1 cut(s)	134
BsiHKAI	GWGCWC	1 cut(s)	1375
BsiSI	CCGG	7 cut(s)	16, 27, 37, 48, 85, 135, 234
BslFI	GGGAC	2 cut(s)	92, 148
BslI	CCNNNNNNNGG	1 cut(s)	1310
BsmAI	GTCTC	1 cut(s)	1877
BsmFI	GGGAC	2 cut(s)	92, 148
BsnI	GGCC	3 cut(s)	453, 724, 1395
Bso31I	GGTCTC	1 cut(s)	1877
Bsp1286I	GDGCHC	2 cut(s)	802, 1375
Bsp13I	TCCGGA	1 cut(s)	15
Bsp143I	GATC	3 cut(s)	923, 1984, 2050
Bsp1720I	GCTNAGC	1 cut(s)	441
Bsp19I	CCATGG	2 cut(s)	267, 1094
BspANI	GGCC	3 cut(s)	453, 724, 1395
BspCNI	CTCAG	2 cut(s)	433, 919
BspEI	TCCGGA	1 cut(s)	15
BspHI	TCATGA	2 cut(s)	895, 1513
BspLI	GGNNCC	7 cut(s)	52, 72, 89, 1271, 1576, 1720, 1986
BspMI	ACCTGC	1 cut(s)	1907
BspOI	GCTAGC	1 cut(s)	905
BspPI	GGATC	3 cut(s)	1979, 1992, 2045
BspT107I	GGYRCC	5 cut(s)	50, 87, 1269, 1574, 1718
BspTNI	GGTCTC	1 cut(s)	1877
BsrDI	GCAATG	3 cut(s)	228, 1125, 1489
BsrFI	RCCGGY	1 cut(s)	134
BsrI	ACTGG	3 cut(s)	1503, 1681, 1883
BssAI	RCCGGY	1 cut(s)	134
BssECI	CCNNGG	6 cut(s)	267, 616, 725, 726, 1094, 1578
BssMI	GATC	3 cut(s)	923, 1984, 2050
BssNAI	GTATAC	2 cut(s)	650, 1848
BssNI	GRCGYC	1 cut(s)	88
BssSI	CACGAG	2 cut(s)	852, 1979
BssT1I	CCWWGG	3 cut(s)	267, 616, 1094
Bst1107I	GTATAC	2 cut(s)	650, 1848
Bst2BI	CACGAG	2 cut(s)	852, 1979
Bst2UI	CCWGG	4 cut(s)	727, 1257, 1365, 1579
Bst4CI	ACNGT	6 cut(s)	289, 329, 582, 591, 1845, 2072
Bst6I	CTCTTC	3 cut(s)	1156, 1182, 1302
BstACI	GRCGYC	1 cut(s)	88
BstAPI	GCANNNNNTGC	1 cut(s)	230
BstBAI	YACGTR	1 cut(s)	647
BstC8I	GCNNGC	1 cut(s)	903
BstDEI	CTNAG	5 cut(s)	5, 441, 863, 906, 1692
BstDSI	CCRYGG	2 cut(s)	267, 1094
BstF5I	GGATG	7 cut(s)	243, 277, 331, 368, 1105, 1242, 1300
BstH2I	RGCGCY	2 cut(s)	91, 1023
BstHHI	GCGC	2 cut(s)	90, 1022
BstKTI	GATC	3 cut(s)	926, 1987, 2053
BstMAI	GTCTC	1 cut(s)	1877
BstMBI	GATC	3 cut(s)	923, 1984, 2050
BstMWI	GCNNNNNNNGC	3 cut(s)	230, 365, 1379
BstNI	CCWGG	4 cut(s)	727, 1257, 1365, 1579
BstNSI	RCATGY	1 cut(s)	1529
BstSCI	CCNGG	7 cut(s)	46, 83, 233, 725, 1255, 1363, 1577
BstSFI	CTRYAG	1 cut(s)	624
BstSLI	GKGCMC	1 cut(s)	802
BstV1I	GCAGC	2 cut(s)	1723, 1756
BstV2I	GAAGAC	3 cut(s)	2, 1122, 1658
BstX2I	RGATCY	3 cut(s)	923, 1984, 2050
BstYI	RGATCY	3 cut(s)	923, 1984, 2050
BstZ17I	GTATAC	2 cut(s)	650, 1848
BsuI	GTATCC	1 cut(s)	1371
BsuRI	GGCC	3 cut(s)	453, 724, 1395
BtgI	CCRYGG	2 cut(s)	267, 1094
BtgZI	GCGATG	1 cut(s)	85
BtrI	CACGTC	1 cut(s)	1463
BtsCI	GGATG	7 cut(s)	243, 277, 331, 368, 1105, 1242, 1300
BtsIMutI	CAGTG	1 cut(s)	609
BveI	ACCTGC	1 cut(s)	1907
Cac8I	GCNNGC	1 cut(s)	903
CaiI	CAGNNNCTG	1 cut(s)	1972
CciI	TCATGA	2 cut(s)	895, 1513
CfoI	GCGC	2 cut(s)	90, 1022
Cfr10I	RCCGGY	1 cut(s)	134
Cfr13I	GGNCC	4 cut(s)	21, 70, 451, 723
CsiI	ACCWGGT	1 cut(s)	1255
Csp6I	GTAC	5 cut(s)	40, 1330, 1342, 1575, 1624
CspAI	ACCGGT	1 cut(s)	134
CviQI	GTAC	5 cut(s)	40, 1330, 1342, 1575, 1624
DdeI	CTNAG	5 cut(s)	5, 441, 863, 906, 1692
DinI	GGCGCC	1 cut(s)	89
DpnI	GATC	3 cut(s)	925, 1986, 2052
DpnII	GATC	3 cut(s)	923, 1984, 2050
DraI	TTTAAA	1 cut(s)	1036
DraIII	CACNNNGTG	1 cut(s)	1259
EaeI	YGGCCR	1 cut(s)	1393
Eam1104I	CTCTTC	3 cut(s)	1156, 1182, 1302
EarI	CTCTTC	3 cut(s)	1156, 1182, 1302
EciI	GGCGGA	4 cut(s)	13, 112, 266, 485
Eco130I	CCWWGG	3 cut(s)	267, 616, 1094
Eco31I	GGTCTC	1 cut(s)	1877
Eco32I	GATATC	1 cut(s)	2125
Eco47I	GGWCC	2 cut(s)	21, 70
Eco47III	AGCGCT	1 cut(s)	1021
Eco57I	CTGAAG	1 cut(s)	1326
EcoRI	GAATTC	2 cut(s)	344, 519
EcoRII	CCWGG	4 cut(s)	725, 1255, 1363, 1577
EcoRV	GATATC	1 cut(s)	2125
EcoT14I	CCWWGG	3 cut(s)	267, 616, 1094
EgeI	GGCGCC	1 cut(s)	89
EheI	GGCGCC	1 cut(s)	89
ErhI	CCWWGG	3 cut(s)	267, 616, 1094
FaqI	GGGAC	2 cut(s)	92, 148
FblI	GTMKAC	3 cut(s)	189, 649, 1847
Fnu4HI	GCNGC	4 cut(s)	1396, 1737, 1770, 1953
FokI	GGATG	7 cut(s)	250, 284, 318, 355, 1112, 1229, 1287
Fsp4HI	GCNGC	4 cut(s)	1396, 1737, 1770, 1953
FspBI	CTAG	4 cut(s)	902, 1017, 1250, 1851
GlaI	GCGC	2 cut(s)	89, 1021
GluI	GCNGC	4 cut(s)	1396, 1737, 1770, 1953
GsaI	CCCAGC	2 cut(s)	641, 2156
HaeII	RGCGCY	2 cut(s)	91, 1023
HaeIII	GGCC	3 cut(s)	453, 724, 1395
HapII	CCGG	7 cut(s)	16, 27, 37, 48, 85, 135, 234
HhaI	GCGC	2 cut(s)	90, 1022
Hin1I	GRCGYC	1 cut(s)	88
Hin6I	GCGC	2 cut(s)	88, 1020
HinP1I	GCGC	2 cut(s)	88, 1020
HincII	GTYRAC	1 cut(s)	190
HindII	GTYRAC	1 cut(s)	190
HinfI	GANTC	9 cut(s)	7, 305, 713, 1148, 1214, 1262, 1409, 1706, 1890
HpaII	CCGG	7 cut(s)	16, 27, 37, 48, 85, 135, 234
HphI	GGTGA	8 cut(s)	957, 1271, 1325, 1594, 1691, 1889, 2119, 2170
Hpy166II	GTNNAC	3 cut(s)	190, 650, 1848
Hpy188I	TCNGA	9 cut(s)	70, 283, 402, 532, 909, 1013, 1408, 1414, 1758
Hpy8I	GTNNAC	3 cut(s)	190, 650, 1848
Hpy99I	CGWCG	4 cut(s)	191, 194, 250, 2144
HpyAV	CCTTC	5 cut(s)	252, 505, 1345, 1617, 2155
HpyCH4III	ACNGT	6 cut(s)	289, 329, 582, 591, 1845, 2072
HpyCH4IV	ACGT	4 cut(s)	181, 646, 1462, 1753
HpyF10VI	GCNNNNNNNGC	3 cut(s)	230, 365, 1379
HpyF3I	CTNAG	5 cut(s)	5, 441, 863, 906, 1692
HpySE526I	ACGT	4 cut(s)	181, 646, 1462, 1753
Hsp92I	GRCGYC	1 cut(s)	88
HspAI	GCGC	2 cut(s)	88, 1020
KasI	GGCGCC	1 cut(s)	87
Kpn2I	TCCGGA	1 cut(s)	15
KpnI	GGTACC	1 cut(s)	1578
Kzo9I	GATC	3 cut(s)	923, 1984, 2050
LmnI	GCTCC	2 cut(s)	39, 2042
Lsp1109I	GCAGC	2 cut(s)	1723, 1756
LweI	GCATC	6 cut(s)	184, 228, 377, 650, 1172, 1251
MabI	ACCWGGT	1 cut(s)	1255
MaeI	CTAG	4 cut(s)	902, 1017, 1250, 1851
MaeII	ACGT	4 cut(s)	181, 646, 1462, 1753
MaeIII	GTNAC	4 cut(s)	1895, 1976, 2028, 2102
MalI	GATC	3 cut(s)	925, 1986, 2052
MboI	GATC	3 cut(s)	923, 1984, 2050
MboII	GAAGA	6 cut(s)	1122, 1143, 1169, 1319, 1663, 1780
MfeI	CAATTG	1 cut(s)	455
MflI	RGATCY	3 cut(s)	923, 1984, 2050
MhlI	GDGCHC	2 cut(s)	802, 1375
Mly113I	GGCGCC	1 cut(s)	88
MlyI	GAGTC	4 cut(s)	16, 299, 1142, 1715
MmeI	TCCRAC	1 cut(s)	1835
MroI	TCCGGA	1 cut(s)	15
MseI	TTAA	8 cut(s)	158, 675, 1035, 1053, 1386, 1997, 2015, 2066
MslI	CAYNNNNRTG	3 cut(s)	377, 1118, 1275
MspI	CCGG	7 cut(s)	16, 27, 37, 48, 85, 135, 234
MspR9I	CCNGG	7 cut(s)	48, 85, 235, 727, 1257, 1365, 1579
MunI	CAATTG	1 cut(s)	455
MvaI	CCWGG	4 cut(s)	727, 1257, 1365, 1579
MwoI	GCNNNNNNNGC	3 cut(s)	230, 365, 1379
NarI	GGCGCC	1 cut(s)	88
NciI	CCSGG	3 cut(s)	48, 85, 235
NcoI	CCATGG	2 cut(s)	267, 1094
NdeII	GATC	3 cut(s)	923, 1984, 2050
NheI	GCTAGC	1 cut(s)	901
NlaIV	GGNNCC	7 cut(s)	52, 72, 89, 1271, 1576, 1720, 1986
NmuCI	GTSAC	2 cut(s)	1895, 1976
NspI	RCATGY	1 cut(s)	1529
PagI	TCATGA	2 cut(s)	895, 1513
PasI	CCCWGGG	1 cut(s)	726
PcsI	WCGNNNNNNNCGW	1 cut(s)	198
PfeI	GAWTC	5 cut(s)	713, 1214, 1262, 1409, 1890
PinAI	ACCGGT	1 cut(s)	134
PkrI	GCNGC	4 cut(s)	1397, 1738, 1771, 1954
PleI	GAGTC	4 cut(s)	15, 299, 1142, 1714
PluTI	GGCGCC	1 cut(s)	91
PpsI	GAGTC	4 cut(s)	15, 299, 1142, 1714
Ppu21I	YACGTR	1 cut(s)	647
Psp1406I	AACGTT	1 cut(s)	181
Psp6I	CCWGG	4 cut(s)	725, 1255, 1363, 1577
PspFI	CCCAGC	2 cut(s)	637, 2152
PspGI	CCWGG	4 cut(s)	725, 1255, 1363, 1577
PspN4I	GGNNCC	7 cut(s)	52, 72, 89, 1271, 1576, 1720, 1986
PspPI	GGNCC	4 cut(s)	21, 70, 451, 723
PstNI	CAGNNNCTG	1 cut(s)	1972
PsuI	RGATCY	3 cut(s)	923, 1984, 2050
RsaI	GTAC	5 cut(s)	41, 1331, 1343, 1576, 1625
RsaNI	GTAC	5 cut(s)	40, 1330, 1342, 1575, 1624
RseI	CAYNNNNRTG	3 cut(s)	377, 1118, 1275
SalI	GTCGAC	1 cut(s)	188
SaqAI	TTAA	8 cut(s)	158, 675, 1035, 1053, 1386, 1997, 2015, 2066
SatI	GCNGC	4 cut(s)	1396, 1737, 1770, 1953
Sau3AI	GATC	3 cut(s)	923, 1984, 2050
Sau96I	GGNCC	4 cut(s)	21, 70, 451, 723
ScaI	AGTACT	1 cut(s)	1625
SchI	GAGTC	4 cut(s)	16, 299, 1142, 1715
ScrFI	CCNGG	7 cut(s)	48, 85, 235, 727, 1257, 1365, 1579
SduI	GDGCHC	2 cut(s)	802, 1375
SexAI	ACCWGGT	1 cut(s)	1255
SfaNI	GCATC	6 cut(s)	184, 228, 377, 650, 1172, 1251
SfcI	CTRYAG	1 cut(s)	624
SfoI	GGCGCC	1 cut(s)	89
SgrDI	CGTCGACG	1 cut(s)	188
SinI	GGWCC	2 cut(s)	21, 70
SmiMI	CAYNNNNRTG	3 cut(s)	377, 1118, 1275
SmlI	CTYRAG	1 cut(s)	1345
SmoI	CTYRAG	1 cut(s)	1345
SpeI	ACTAGT	1 cut(s)	1850
SspDI	GGCGCC	1 cut(s)	87
SspI	AATATT	1 cut(s)	604
SspMI	CTAG	4 cut(s)	902, 1017, 1250, 1851
StyD4I	CCNGG	7 cut(s)	46, 83, 233, 725, 1255, 1363, 1577
StyI	CCWWGG	3 cut(s)	267, 616, 1094
TaaI	ACNGT	6 cut(s)	289, 329, 582, 591, 1845, 2072
TaiI	ACGT	4 cut(s)	184, 649, 1465, 1756
TaqI	TCGA	6 cut(s)	150, 189, 201, 322, 873, 1191
TatI	WGTACW	2 cut(s)	1341, 1623
TauI	GCSGC	2 cut(s)	1398, 1955
TfiI	GAWTC	5 cut(s)	713, 1214, 1262, 1409, 1890
Tru1I	TTAA	8 cut(s)	158, 675, 1035, 1053, 1386, 1997, 2015, 2066
Tru9I	TTAA	8 cut(s)	158, 675, 1035, 1053, 1386, 1997, 2015, 2066
TscAI	CASTG	1 cut(s)	616
TseFI	GTSAC	2 cut(s)	1895, 1976
TseI	GCWGC	2 cut(s)	1736, 1769
Tsp45I	GTSAC	2 cut(s)	1895, 1976
TspGWI	ACGGA	2 cut(s)	1568, 2121
TspRI	CASTG	1 cut(s)	616
VpaK11BI	GGWCC	2 cut(s)	21, 70
XapI	RAATTY	7 cut(s)	276, 344, 519, 570, 658, 1049, 2088
XceI	RCATGY	1 cut(s)	1529
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1263
XmiI	GTMKAC	3 cut(s)	189, 649, 1847
XspI	CTAG	4 cut(s)	902, 1017, 1250, 1851
ZrmI	AGTACT	1 cut(s)	1625

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.