MD07G1257400.v1.1

Hypersensitive-induced response protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015410
Physical Location & Seq
Forward (+)
32368433 .. 32370691
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1257400.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGGCAATTCGTGCTGCTGCTTCTGTATCTGCATAGAGCAAGCGAGCATAGGCATCTTGGAGAGGTGGGGCCGTTTCGAGAGGCTCGCCGAACCCGGTTTCCACCTCCTGAACCCATTGGCCGGTCAATGGGTCGCCGGCATCCTCTCCACCAGGATCGCCTCCCTTGATGTCCGTATCGAGACCAAAACCAAGGATAATGTGTTTGTGCAATTGGTGTGCTCAATTCAATACAGGGTGGTGAAAGAAAATGCTGATGATGCGTTTTATGAGCTGCAAAACCCCAAAGAGCAGATTCAAGCTTATGTCTTTGATGTGGTTAGAGCTCTGGTTCCGAGAATGACACTGGACGAGCTCTTTGAGCAGAAGGGTGAGGTTGCTAAAGCCGTGTTGGAGGAGCTTGAGAAGGTTATGAGAGAATATGGATACAGCATAGAGCACATGCTGATGGTTGACATTATACCTGATGCCTCTGTCCGCAAGGCAATGAATGAGATCAATGCAGCTCAAAGGCTCCAGCTAGCCAATGTATACAAAGGAGAAGCAGAGAAGGTGCTTCAGGTGAAAAGGGCAGAAGCTGAAGCTGAGGCCAAGTACCTTGGCGGAGTTGGTGTTGCCAGGCAGAGATCGGTGATCAAATACGCAACGGACTAA

Protein Analysis

218

Amino Acids

24.51

Weight (kDa)

5.45

Isoelectric Point (pI)

30.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 12 - 184 1.2e-27 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51570
fragaria_vesca FvH4_7g28140
malus_domestica MD01G1185100.v1.1 MD07G1257400.v1.1
prunus_persica Prupe.2G281600_v2.0.a1
pyrus_communis pycom01g19680 pycom07g23040
rosa_chinensis RchiOBHm_Chr1g0375061
rosa_laevigata RLG00000026691
rosa_multiflora Rmu_sc0000554.1_g000014
rosa_roxburghii Rroxscaffold_4G00283050
rosa_rugosa Rorug01G0387900
rosa_samantha Rh1AG398500 Rh1BG362600 Rh1CG376200
rosa_wichuraiana Rw1G035380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 531
AciI CCGC 2 cut(s) 478, 603
AclWI GGATC 1 cut(s) 164
AcoI YGGCCR 1 cut(s) 120
AcuI CTGAAG 2 cut(s) 542, 600
AfaI GTAC 1 cut(s) 596
AfiI CCNNNNNNNGG 2 cut(s) 122, 129
AgsI TTSAA 2 cut(s) 230, 299
AjnI CCWGG 2 cut(s) 152, 617
AloI GAACNNNNNNTCC 2 cut(s) 84, 116
AluBI AGCT 9 cut(s) 274, 302, 326, 355, 400, 506, 520, 578, 584
AluI AGCT 9 cut(s) 274, 302, 326, 355, 400, 506, 520, 578, 584
Alw21I GWGCWC 4 cut(s) 224, 328, 357, 441
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 1 cut(s) 164
AlwNI CAGNNNCTG 1 cut(s) 578
AoxI GGCC 3 cut(s) 70, 120, 588
ApeKI GCWGC 4 cut(s) 15, 18, 274, 503
ArsI GACNNNNNNTTYG 2 cut(s) 341, 373
AspS9I GGNCC 1 cut(s) 70
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 4 cut(s) 253, 383, 574, 643
AsuNHI GCTAGC 1 cut(s) 520
BanII GRGCYC 2 cut(s) 328, 357
Bbv12I GWGCWC 4 cut(s) 224, 328, 357, 441
BbvCI CCTCAGC 1 cut(s) 585
BbvI GCAGC 4 cut(s) 2, 5, 261, 515
BccI CCATC 1 cut(s) 442
BceAI ACGGC 2 cut(s) 57, 371
BciT130I CCWGG 2 cut(s) 154, 619
BciVI GTATCC 1 cut(s) 419
BclI TGATCA 1 cut(s) 633
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 176
BfaI CTAG 1 cut(s) 521
BfuI GTATCC 1 cut(s) 419
BisI GCNGC 4 cut(s) 16, 19, 275, 504
BlsI GCNGC 4 cut(s) 17, 20, 276, 505
Bme1390I CCNGG 3 cut(s) 96, 154, 619
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 3 cut(s) 71, 333, 515
BmrFI CCNGG 3 cut(s) 96, 154, 619
BmsI GCATC 4 cut(s) 63, 150, 250, 457
BmtI GCTAGC 1 cut(s) 524
BpmI CTGGAG 1 cut(s) 500
Bpu10I CCTNAGC 1 cut(s) 585
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 192, 598
BsaXI ACNNNNNCTCC 2 cut(s) 597, 627
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 129
Bse118I RCCGGY 2 cut(s) 122, 137
Bse1I ACTGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 492
BseBI CCWGG 2 cut(s) 154, 619
BseDI CCNNGG 2 cut(s) 192, 598
BseGI GGATG 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 122, 129
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 1 cut(s) 576
BseNI ACTGG 1 cut(s) 351
BseRI GAGGAG 1 cut(s) 410
BseXI GCAGC 4 cut(s) 2, 5, 261, 515
BshFI GGCC 3 cut(s) 72, 122, 590
BsiHKAI GWGCWC 4 cut(s) 224, 328, 357, 441
BsiSI CCGG 3 cut(s) 96, 123, 138
BslI CCNNNNNNNGG 2 cut(s) 122, 129
BsmAI GTCTC 1 cut(s) 176
BsnI GGCC 3 cut(s) 72, 122, 590
Bso31I GGTCTC 1 cut(s) 176
Bsp1286I GDGCHC 4 cut(s) 224, 328, 357, 441
Bsp143I GATC 4 cut(s) 156, 495, 626, 633
BspACI CCGC 2 cut(s) 478, 603
BspANI GGCC 3 cut(s) 72, 122, 590
BspCNI CTCAG 1 cut(s) 577
BspLI GGNNCC 3 cut(s) 71, 333, 515
BspOI GCTAGC 1 cut(s) 524
BspPI GGATC 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 492
BsrFI RCCGGY 2 cut(s) 122, 137
BsrI ACTGG 1 cut(s) 351
BssAI RCCGGY 2 cut(s) 122, 137
BssECI CCNNGG 2 cut(s) 192, 598
BssMI GATC 4 cut(s) 156, 495, 626, 633
BssNAI GTATAC 1 cut(s) 532
BssT1I CCWWGG 2 cut(s) 192, 598
Bst1107I GTATAC 1 cut(s) 532
Bst2UI CCWGG 2 cut(s) 154, 619
BstAPI GCANNNNNTGC 1 cut(s) 12
BstC8I GCNNGC 5 cut(s) 42, 46, 87, 139, 522
BstDEI CTNAG 1 cut(s) 585
BstF5I GGATG 1 cut(s) 141
BstKTI GATC 4 cut(s) 159, 498, 629, 636
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 4 cut(s) 156, 495, 626, 633
BstMWI GCNNNNNNNGC 3 cut(s) 12, 260, 361
BstNI CCWGG 2 cut(s) 154, 619
BstNSI RCATGY 1 cut(s) 445
BstSCI CCNGG 3 cut(s) 94, 152, 617
BstV1I GCAGC 4 cut(s) 2, 5, 261, 515
BstZ17I GTATAC 1 cut(s) 532
BsuI GTATCC 1 cut(s) 419
BsuRI GGCC 3 cut(s) 72, 122, 590
BtsCI GGATG 1 cut(s) 141
BtsIMutI CAGTG 1 cut(s) 344
Cac8I GCNNGC 5 cut(s) 42, 46, 87, 139, 522
CaiI CAGNNNCTG 1 cut(s) 578
Cfr10I RCCGGY 2 cut(s) 122, 137
Cfr13I GGNCC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 595
CviAII CATG 1 cut(s) 442
CviQI GTAC 1 cut(s) 595
DdeI CTNAG 1 cut(s) 585
DpnI GATC 4 cut(s) 158, 497, 628, 635
DpnII GATC 4 cut(s) 156, 495, 626, 633
EaeI YGGCCR 1 cut(s) 120
EciI GGCGGA 1 cut(s) 618
Ecl136II GAGCTC 2 cut(s) 326, 355
Eco130I CCWWGG 2 cut(s) 192, 598
Eco24I GRGCYC 2 cut(s) 328, 357
Eco31I GGTCTC 1 cut(s) 176
Eco53kI GAGCTC 2 cut(s) 326, 355
Eco57I CTGAAG 2 cut(s) 542, 600
EcoICRI GAGCTC 2 cut(s) 326, 355
EcoRII CCWGG 2 cut(s) 152, 617
EcoT14I CCWWGG 2 cut(s) 192, 598
EcoT38I GRGCYC 2 cut(s) 328, 357
ErhI CCWWGG 2 cut(s) 192, 598
FaeI CATG 1 cut(s) 445
FatI CATG 1 cut(s) 441
FbaI TGATCA 1 cut(s) 633
FblI GTMKAC 1 cut(s) 531
Fnu4HI GCNGC 4 cut(s) 16, 19, 275, 504
FokI GGATG 1 cut(s) 128
FriOI GRGCYC 2 cut(s) 328, 357
Fsp4HI GCNGC 4 cut(s) 16, 19, 275, 504
FspBI CTAG 1 cut(s) 521
GluI GCNGC 4 cut(s) 16, 19, 275, 504
GsuI CTGGAG 1 cut(s) 500
HaeIII GGCC 3 cut(s) 72, 122, 590
HapII CCGG 3 cut(s) 96, 123, 138
Hin1II CATG 1 cut(s) 445
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HindIII AAGCTT 1 cut(s) 300
HinfI GANTC 1 cut(s) 295
HpaII CCGG 3 cut(s) 96, 123, 138
HphI GGTGA 4 cut(s) 253, 383, 574, 643
Hpy166II GTNNAC 2 cut(s) 454, 532
Hpy188I TCNGA 1 cut(s) 336
Hpy188III TCNNGA 3 cut(s) 79, 109, 181
Hpy8I GTNNAC 2 cut(s) 454, 532
HpyAV CCTTC 3 cut(s) 361, 400, 544
HpyCH4V TGCA 4 cut(s) 33, 211, 277, 503
HpyF10VI GCNNNNNNNGC 3 cut(s) 12, 260, 361
HpyF3I CTNAG 1 cut(s) 585
Hsp92II CATG 1 cut(s) 445
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 633
Kzo9I GATC 4 cut(s) 156, 495, 626, 633
LmnI GCTCC 2 cut(s) 397, 519
Lsp1109I GCAGC 4 cut(s) 2, 5, 261, 515
LweI GCATC 4 cut(s) 63, 150, 250, 457
MaeI CTAG 1 cut(s) 521
MalI GATC 4 cut(s) 158, 497, 628, 635
MboI GATC 4 cut(s) 156, 495, 626, 633
MfeI CAATTG 1 cut(s) 212
MhlI GDGCHC 4 cut(s) 224, 328, 357, 441
MluCI AATT 3 cut(s) 7, 212, 225
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 9 cut(s) 57, 75, 116, 155, 172, 367, 388, 481, 580
MroNI GCCGGC 1 cut(s) 137
MslI CAYNNNNRTG 1 cut(s) 446
MspI CCGG 3 cut(s) 96, 123, 138
MspR9I CCNGG 3 cut(s) 96, 154, 619
MunI CAATTG 1 cut(s) 212
MvaI CCWGG 2 cut(s) 154, 619
MwoI GCNNNNNNNGC 3 cut(s) 12, 260, 361
NaeI GCCGGC 1 cut(s) 139
NciI CCSGG 1 cut(s) 96
NdeII GATC 4 cut(s) 156, 495, 626, 633
NgoMIV GCCGGC 1 cut(s) 137
NheI GCTAGC 1 cut(s) 520
NlaIII CATG 1 cut(s) 445
NlaIV GGNNCC 3 cut(s) 71, 333, 515
NspI RCATGY 1 cut(s) 445
PdiI GCCGGC 1 cut(s) 139
PfeI GAWTC 1 cut(s) 295
PkrI GCNGC 4 cut(s) 17, 20, 276, 505
Psp124BI GAGCTC 2 cut(s) 328, 357
Psp6I CCWGG 2 cut(s) 152, 617
PspGI CCWGG 2 cut(s) 152, 617
PspN4I GGNNCC 3 cut(s) 71, 333, 515
PspPI GGNCC 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 578
RsaI GTAC 1 cut(s) 596
RsaNI GTAC 1 cut(s) 595
RseI CAYNNNNRTG 1 cut(s) 446
SacI GAGCTC 2 cut(s) 328, 357
SatI GCNGC 4 cut(s) 16, 19, 275, 504
Sau3AI GATC 4 cut(s) 156, 495, 626, 633
Sau96I GGNCC 1 cut(s) 70
ScrFI CCNGG 3 cut(s) 96, 154, 619
SduI GDGCHC 4 cut(s) 224, 328, 357, 441
SfaNI GCATC 4 cut(s) 63, 150, 250, 457
SmiMI CAYNNNNRTG 1 cut(s) 446
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 3 cut(s) 7, 212, 225
SsiI CCGC 2 cut(s) 478, 603
SspMI CTAG 1 cut(s) 521
SstI GAGCTC 2 cut(s) 328, 357
StyD4I CCNGG 3 cut(s) 94, 152, 617
StyI CCWWGG 2 cut(s) 192, 598
TaqI TCGA 2 cut(s) 78, 180
TasI AATT 3 cut(s) 7, 212, 225
TfiI GAWTC 1 cut(s) 295
TscAI CASTG 1 cut(s) 351
TseI GCWGC 4 cut(s) 15, 18, 274, 503
TspDTI ATGAA 1 cut(s) 503
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 1 cut(s) 351
XceI RCATGY 1 cut(s) 445
XmiI GTMKAC 1 cut(s) 531
XspI CTAG 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.