Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0009500

Physical Location & Seq

Genomic Coordinates Reverse (-)

32400199 .. 32404701

Gene Structure 4503 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1257900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2100 bp

ATGGAAACAACATGTGGATCACTTCTATATGAGCTTCAGATAATTTGGAATGAAGTCGGAGAGACTGACAAAGATAGGGATAAGATGTTGCTTGAGCTTGAGCAGGAGTGTCTAGAAGTATACAGAAGAAAGGTAGATCAGGCAAATCGGTGTAGGGCACAGCTACGGCAGGCAATTGCTGATTCGGAAGCAGAACTTGCTGCAATATGTTCTGCAATGGGGGAGCGACCGGTACATATTAGACAGTCTGATCAAAGTGCCGGAAGTTTGAAGGAAGAGCTCAGCAAAATTATTCCACAGCTGGAGGAGATGAAGAAAAGAAAATGTGACAGGAAAAATAACTTTCTGGAAGTTCTAGAGGAGATACGGAAAATTTCAAGTGAGATTAATGGAAGCACAGATTACAGTTCTTCTAAACCGGGTGTGGATGAAACTGATTTGTCATTGAGAAGGCTTGAAGAATTGCACAGACATCTACATGCACTCCAAACTGAGAAGAGTGATCGGCTTAAGCAGATACAGGACCACCTGTGTACATTGAACTCATTTTGCTCAGTTCTTGGTATGGACTACAAACAGACAATTAGTGAGGTTCATCCCAGTTTAGTTGATTCCGAAGGAAGCAAGAATATAAGTAATGATACAATTCAGCGATTGCTTGCTGCAATACAAAAATTGCGGGAGGTTAAATTACAGAGAATGCAGAAGCTTCAAGATCTTGCAACTACCATGTTAGAGCTGTGGAACTTGATGGATACGCCAATTGAGGAGCAACAGATGTTTCAGAATATAACCTGCAATATAGCTGCTTCAGAACATGAGATAACTGAACCAAACACACTCTCTGTGGACTTTATCAATTATGTTGAGGCAGAAGTGTCTCGGTTGGAAGAGTTGAAATCAAGTAAAATGAAAGAGCTTGTTCTTAAGAAGAGATCAGAGCTAGAGGAAATCTGTAGAAAGACACACATGGTCCTAGAAGCAGATAGTGCAATTGACTACATAATTGAAGCTATAGAATCAGGAGATGTGGACCCTGCTTGTGTCCTTGATCAAATTGAGCTTCAGGTTTCAAAGGTTAAAGAGGAAGCTTTTAGCAGGAAAGACATACTTGAAAAAGTTGAGAAGTGGTTGTTTGCTTGTGATGAGGAGTGCTGGCTCGAGGAGTATAACAGGGATGAAAACCGATACAATGCTGGAAGAGGAGCTCATCTTACTCTCAAGCGTGCTGAGAAAGCACGTGCTTTGGTTAATAAACTTCCAGCAATGGTGGATGCATTGGCTTCAAAAACCATATCATGGGAGAAGGAGAGGGGCATTGAGTTCACATATGATGGTATTCGTCTTCTTTCCATGCTTGAAGAGTATACTATATTACGGGAGGAGAAGGAGCAAGAACGTCGAAGGCAGCGGGACCAGAAAAAGATTCAGGGTCAGTTGATAGCTGAACAAGAGGCACTTTACGGGTCAAAGCCAAGTCCATCGAAACCTCAGAGTGTAAAGAAGGGTCCAAGAATGTCAACTGGAGGTGCGAGCAATAGAAGACTTTCAATGCAAGCTCCTAAACCTGATCCACTTCATTCCGTCAAAGGTACTCCGCGTCCGCGTTCAAGAAAGGGTGACCGAGTCCACCAAAATGAGCAATTAAACAATCAGCTGGATGATAGCTTTTCATCTTTGTCCACAGGTAGACGAGGCTTGGATGTTGCTGGACTTCCTGTTAAAAAGCACTCTTTTGGTGCCCCAAATGCTTCTGAAGTGGAGACACCAACGACTCGTAAACCCTTCTCCCCCATTTCTGTTCCCGTTTCCATTCCAACAGAAAATGTCGCTTTCGCTACTACTCCTCTGAAGCCTAGAACTGTTGCAGACGAAGCGAACAAGACGCCCAAGGTGATGCCAATCTTCGTGCCAACGACACCATCAACTGTGTCAGTTCCCATGCAGACAGCTATCACACCAGCTCCTCCTCCTCCACCAATTCCATTTGCTACCAACTTGGTCCAAGAGATTCCTGAGGAGATTGAGTACTCGTTCGAGGAAAGAAGAGCTGGATTTGTGCTCCCTAGAACACATGTGAAGCCAATGATACAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0008017 GO:0008092 GO:0008150 GO:0009524 GO:0009574 GO:0009605 GO:0009607 GO:0009624 GO:0009653 GO:0009987 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0032502 GO:0032506 GO:0034622 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043933 GO:0044002 GO:0044085 GO:0044111 GO:0044115 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0046785 GO:0048285 GO:0048646 GO:0048856 GO:0050896 GO:0051258 GO:0051301 GO:0051701 GO:0051704 GO:0051707 GO:0051816 GO:0052093 GO:0052095 GO:0052096 GO:0055028 GO:0061640 GO:0065003 GO:0071840 GO:0071944 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MAP65_ASE1

Protein Analysis

700

Amino Acids

79.35

Weight (kDa)

5.78

Isoelectric Point (pI)

56.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MAP65_ASE1	PF03999	7 - 498	5.5e-118	Microtubule associated protein (MAP65/ASE1 family)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009500)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51600
fragaria_vesca	FvH4_7g28190 FvH4_7g28190 FvH4_7g28190 FvH4_7g28190
malus_domestica	MD01G1185800.v1.1 MD07G1257900.v1.1
prunus_persica	Prupe.2G282400_v2.0.a1 Prupe.2G282400_v2.0.a1 Prupe.2G282400_v2.0.a1
pyrus_communis	pycom01g19750 pycom07g23100
rosa_chinensis	RchiOBHm_Chr1g0375141
rosa_laevigata	RLG00000026683
rosa_multiflora	Rmu_sc0000554.1_g000022
rosa_roxburghii	Rroxscaffold_4G00282980
rosa_rugosa	Rorug01G0388800
rosa_samantha	Rh1AG399300 Rh1BG365200 Rh1CG378100 Rh1DG395900
rosa_wichuraiana	Rw1G035450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	803
AccB1I	GGYRCC	1 cut(s)	1739
AccB7I	CCANNNNNTGG	1 cut(s)	1299
AccI	GTMKAC	3 cut(s)	120, 1367, 1690
AccII	CGCG	2 cut(s)	1600, 1606
AciI	CCGC	4 cut(s)	679, 1411, 1598, 1604
AclWI	GGATC	2 cut(s)	25, 1565
AcsI	RAATTY	1 cut(s)	372
AcuI	CTGAAG	5 cut(s)	20, 795, 1049, 1776, 1871
AcvI	CACGTG	1 cut(s)	1241
AcyI	GRCGYC	1 cut(s)	1886
AfaI	GTAC	4 cut(s)	234, 535, 1594, 2030
AfiI	CCNNNNNNNGG	1 cut(s)	1299
AflII	CTTAAG	2 cut(s)	509, 926
AflIII	ACRYGT	2 cut(s)	11, 2074
AgeI	ACCGGT	1 cut(s)	229
Alw21I	GWGCWC	3 cut(s)	282, 1210, 2064
Alw26I	GTCTC	3 cut(s)	56, 885, 1757
AlwI	GGATC	2 cut(s)	25, 1565
Ama87I	CYCGRG	1 cut(s)	1160
ApeKI	GCWGC	4 cut(s)	200, 662, 806, 1408
ApoI	RAATTY	1 cut(s)	372
AseI	ATTAAT	1 cut(s)	387
AsiGI	ACCGGT	1 cut(s)	229
Asp700I	GAANNNNTTC	1 cut(s)	1546
AspS9I	GGNCC	6 cut(s)	523, 973, 1033, 1414, 1508, 2002
AsuC2I	CCSGG	1 cut(s)	420
AsuHPI	GGTGA	2 cut(s)	1631, 1906
AvaI	CYCGRG	1 cut(s)	1160
AvaII	GGWCC	6 cut(s)	523, 973, 1033, 1414, 1508, 2002
AxyI	CCTNAGG	1 cut(s)	2016
BaeGI	GKGCMC	2 cut(s)	160, 1744
BanI	GGYRCC	1 cut(s)	1739
BanII	GRGCYC	2 cut(s)	282, 1210
BbrPI	CACGTG	1 cut(s)	1241
BbsI	GAAGAC	2 cut(s)	1337, 1549
Bbv12I	GWGCWC	3 cut(s)	282, 1210, 2064
BbvI	GCAGC	4 cut(s)	187, 649, 793, 1420
BccI	CCATC	4 cut(s)	745, 1328, 1489, 1930
BceAI	ACGGC	1 cut(s)	182
BcgI	CGANNNNNNTGC	2 cut(s)	1382, 1416
BciVI	GTATCC	1 cut(s)	748
BclI	TGATCA	2 cut(s)	250, 1051
BcnI	CCSGG	1 cut(s)	420
BcoDI	GTCTC	3 cut(s)	56, 885, 1757
BfaI	CTAG	6 cut(s)	113, 356, 944, 977, 1857, 2067
BfmI	CTRYAG	2 cut(s)	955, 1014
BfrI	CTTAAG	2 cut(s)	509, 926
BfuAI	ACCTGC	1 cut(s)	803
BfuI	GTATCC	1 cut(s)	748
BglII	AGATCT	1 cut(s)	715
BisI	GCNGC	4 cut(s)	201, 663, 807, 1409
BlpI	GCTNAGC	1 cut(s)	281
BlsI	GCNGC	4 cut(s)	202, 664, 808, 1410
BmcAI	AGTACT	1 cut(s)	2030
Bme1390I	CCNGG	1 cut(s)	420
Bme18I	GGWCC	6 cut(s)	523, 973, 1033, 1414, 1508, 2002
BmeT110I	CYCGRG	1 cut(s)	1160
BmgT120I	GGNCC	6 cut(s)	523, 973, 1033, 1414, 1508, 2002
BmiI	GGNNCC	4 cut(s)	1035, 1415, 1509, 1741
BmrFI	CCNGG	1 cut(s)	420
BmrI	ACTGGG	1 cut(s)	594
BmsI	GCATC	2 cut(s)	1264, 1887
BmuI	ACTGGG	1 cut(s)	594
BpiI	GAAGAC	2 cut(s)	1337, 1549
BpmI	CTGGAG	2 cut(s)	323, 1545
Bpu1102I	GCTNAGC	1 cut(s)	281
BpuEI	CTTGAG	3 cut(s)	113, 119, 1205
BpuMI	CCSGG	1 cut(s)	420
BsaAI	YACGTR	1 cut(s)	1241
BsaBI	GATNNNNATC	1 cut(s)	1901
BsaHI	GRCGYC	1 cut(s)	1886
BsaJI	CCNNGG	1 cut(s)	1890
BsaWI	WCCGGW	1 cut(s)	229
BsaXI	ACNNNNNCTCC	6 cut(s)	468, 498, 1948, 1978, 2012, 2042
Bsc4I	CCNNNNNNNGG	1 cut(s)	1299
Bse118I	RCCGGY	1 cut(s)	229
Bse1I	ACTGG	2 cut(s)	600, 1528
Bse21I	CCTNAGG	1 cut(s)	2016
Bse3DI	GCAATG	2 cut(s)	222, 1272
Bse8I	GATNNNNATC	1 cut(s)	1901
BseDI	CCNNGG	1 cut(s)	1890
BseGI	GGATG	6 cut(s)	433, 595, 1183, 1279, 1666, 1708
BseJI	GATNNNNATC	1 cut(s)	1901
BseLI	CCNNNNNNNGG	1 cut(s)	1299
BseMI	GCAATG	2 cut(s)	222, 1272
BseMII	CTCAG	6 cut(s)	295, 483, 567, 1221, 1505, 2007
BseNI	ACTGG	2 cut(s)	600, 1528
BseSI	GKGCMC	2 cut(s)	160, 1744
BseXI	GCAGC	4 cut(s)	187, 649, 793, 1420
Bsh1236I	CGCG	2 cut(s)	1600, 1606
Bsh1285I	CGRYCG	1 cut(s)	230
BshNI	GGYRCC	1 cut(s)	1739
BshTI	ACCGGT	1 cut(s)	229
BsiEI	CGRYCG	1 cut(s)	230
BsiHKAI	GWGCWC	3 cut(s)	282, 1210, 2064
BsiHKCI	CYCGRG	1 cut(s)	1160
BsiSI	CCGG	3 cut(s)	230, 261, 419
BslFI	GGGAC	1 cut(s)	1427
BslI	CCNNNNNNNGG	1 cut(s)	1299
BsmAI	GTCTC	3 cut(s)	56, 885, 1757
BsmFI	GGGAC	1 cut(s)	1427
BsmI	GAATGC	1 cut(s)	705
BsoBI	CYCGRG	1 cut(s)	1160
Bsp1286I	GDGCHC	5 cut(s)	160, 282, 1210, 1744, 2064
Bsp1407I	TGTACA	1 cut(s)	533
Bsp143I	GATC	8 cut(s)	17, 136, 250, 502, 715, 935, 1051, 1570
Bsp1720I	GCTNAGC	1 cut(s)	281
BspACI	CCGC	4 cut(s)	679, 1411, 1598, 1604
BspCNI	CTCAG	6 cut(s)	294, 484, 566, 1222, 1504, 2008
BspFNI	CGCG	2 cut(s)	1600, 1606
BspLI	GGNNCC	4 cut(s)	1035, 1415, 1509, 1741
BspMI	ACCTGC	1 cut(s)	803
BspPI	GGATC	2 cut(s)	25, 1565
BspQI	GCTCTTC	2 cut(s)	270, 2041
BspT107I	GGYRCC	1 cut(s)	1739
BspTI	CTTAAG	2 cut(s)	509, 926
BsrDI	GCAATG	2 cut(s)	222, 1272
BsrFI	RCCGGY	1 cut(s)	229
BsrGI	TGTACA	1 cut(s)	533
BsrI	ACTGG	2 cut(s)	600, 1528
BssAI	RCCGGY	1 cut(s)	229
BssECI	CCNNGG	1 cut(s)	1890
BssMI	GATC	8 cut(s)	17, 136, 250, 502, 715, 935, 1051, 1570
BssNAI	GTATAC	2 cut(s)	121, 1368
BssNI	GRCGYC	1 cut(s)	1886
BssT1I	CCWWGG	1 cut(s)	1890
Bst1107I	GTATAC	2 cut(s)	121, 1368
Bst4CI	ACNGT	4 cut(s)	246, 407, 1864, 1930
Bst6I	CTCTTC	7 cut(s)	270, 491, 885, 926, 1195, 1356, 2041
BstACI	GRCGYC	1 cut(s)	1886
BstAFI	CTTAAG	2 cut(s)	509, 926
BstAPI	GCANNNNNTGC	2 cut(s)	197, 989
BstAUI	TGTACA	1 cut(s)	533
BstBAI	YACGTR	1 cut(s)	1241
BstC8I	GCNNGC	6 cut(s)	171, 660, 1157, 1227, 1534, 1557
BstDEI	CTNAG	6 cut(s)	281, 492, 553, 1230, 1491, 2016
BstEII	GGTNACC	1 cut(s)	1619
BstF5I	GGATG	6 cut(s)	433, 595, 1183, 1279, 1666, 1708
BstFNI	CGCG	2 cut(s)	1600, 1606
BstKTI	GATC	8 cut(s)	20, 139, 253, 505, 718, 938, 1054, 1573
BstMAI	GTCTC	3 cut(s)	56, 885, 1757
BstMBI	GATC	8 cut(s)	17, 136, 250, 502, 715, 935, 1051, 1570
BstMCI	CGRYCG	1 cut(s)	230
BstMWI	GCNNNNNNNGC	5 cut(s)	197, 989, 1235, 1748, 1874
BstNSI	RCATGY	3 cut(s)	15, 482, 2078
BstPI	GGTNACC	1 cut(s)	1619
BstSCI	CCNGG	1 cut(s)	418
BstSFI	CTRYAG	2 cut(s)	955, 1014
BstSLI	GKGCMC	2 cut(s)	160, 1744
BstUI	CGCG	2 cut(s)	1600, 1606
BstV1I	GCAGC	4 cut(s)	187, 649, 793, 1420
BstV2I	GAAGAC	2 cut(s)	1337, 1549
BstX2I	RGATCY	1 cut(s)	715
BstYI	RGATCY	1 cut(s)	715
BstZ17I	GTATAC	2 cut(s)	121, 1368
Bsu36I	CCTNAGG	1 cut(s)	2016
BsuI	GTATCC	1 cut(s)	748
BtsCI	GGATG	6 cut(s)	433, 595, 1183, 1279, 1666, 1708
BveI	ACCTGC	1 cut(s)	803
Cac8I	GCNNGC	6 cut(s)	171, 660, 1157, 1227, 1534, 1557
Cfr10I	RCCGGY	1 cut(s)	229
Cfr13I	GGNCC	6 cut(s)	523, 973, 1033, 1414, 1508, 2002
CseI	GACGC	2 cut(s)	1589, 1894
Csp6I	GTAC	4 cut(s)	233, 534, 1593, 2029
CspAI	ACCGGT	1 cut(s)	229
CviAII	CATG	9 cut(s)	12, 479, 730, 818, 970, 1299, 1354, 1942, 2075
CviQI	GTAC	4 cut(s)	233, 534, 1593, 2029
DdeI	CTNAG	6 cut(s)	281, 492, 553, 1230, 1491, 2016
DpnI	GATC	8 cut(s)	19, 138, 252, 504, 717, 937, 1053, 1572
DpnII	GATC	8 cut(s)	17, 136, 250, 502, 715, 935, 1051, 1570
Eam1104I	CTCTTC	7 cut(s)	270, 491, 885, 926, 1195, 1356, 2041
EarI	CTCTTC	7 cut(s)	270, 491, 885, 926, 1195, 1356, 2041
Ecl136II	GAGCTC	2 cut(s)	280, 1208
Eco130I	CCWWGG	1 cut(s)	1890
Eco24I	GRGCYC	2 cut(s)	282, 1210
Eco47I	GGWCC	6 cut(s)	523, 973, 1033, 1414, 1508, 2002
Eco53kI	GAGCTC	2 cut(s)	280, 1208
Eco57I	CTGAAG	5 cut(s)	20, 795, 1049, 1776, 1871
Eco72I	CACGTG	1 cut(s)	1241
Eco81I	CCTNAGG	1 cut(s)	2016
Eco88I	CYCGRG	1 cut(s)	1160
Eco91I	GGTNACC	1 cut(s)	1619
EcoICRI	GAGCTC	2 cut(s)	280, 1208
EcoO65I	GGTNACC	1 cut(s)	1619
EcoT14I	CCWWGG	1 cut(s)	1890
EcoT22I	ATGCAT	1 cut(s)	1279
EcoT38I	GRGCYC	2 cut(s)	282, 1210
ErhI	CCWWGG	1 cut(s)	1890
FaeI	CATG	9 cut(s)	15, 482, 733, 821, 973, 1302, 1357, 1945, 2078
FalI	AAGNNNNNCTT	6 cut(s)	180, 212, 1095, 1127, 1443, 1475
FaqI	GGGAC	1 cut(s)	1427
FatI	CATG	9 cut(s)	11, 478, 729, 817, 969, 1298, 1353, 1941, 2074
FauI	CCCGC	2 cut(s)	672, 1404
FauNDI	CATATG	1 cut(s)	1330
FbaI	TGATCA	2 cut(s)	250, 1051
FblI	GTMKAC	3 cut(s)	120, 1367, 1690
Fnu4HI	GCNGC	4 cut(s)	201, 663, 807, 1409
FokI	GGATG	6 cut(s)	440, 582, 1190, 1286, 1673, 1715
FriOI	GRGCYC	2 cut(s)	282, 1210
Fsp4HI	GCNGC	4 cut(s)	201, 663, 807, 1409
FspBI	CTAG	6 cut(s)	113, 356, 944, 977, 1857, 2067
GluI	GCNGC	4 cut(s)	201, 663, 807, 1409
GsuI	CTGGAG	2 cut(s)	323, 1545
HapII	CCGG	3 cut(s)	230, 261, 419
HgaI	GACGC	2 cut(s)	1589, 1894
Hin1I	GRCGYC	1 cut(s)	1886
Hin1II	CATG	9 cut(s)	15, 482, 733, 821, 973, 1302, 1357, 1945, 2078
HincII	GTYRAC	1 cut(s)	1521
HindII	GTYRAC	1 cut(s)	1521
HindIII	AAGCTT	2 cut(s)	707, 1089
HinfI	GANTC	7 cut(s)	182, 611, 1019, 1426, 1626, 1774, 2011
HpaII	CCGG	3 cut(s)	230, 261, 419
HphI	GGTGA	2 cut(s)	1631, 1906
Hpy188III	TCNNGA	7 cut(s)	113, 347, 356, 713, 1023, 1611, 2015
Hpy99I	CGWCG	1 cut(s)	1404
HpyAV	CCTTC	8 cut(s)	265, 444, 611, 1300, 1381, 1398, 1498, 1795
HpyCH4III	ACNGT	4 cut(s)	246, 407, 1864, 1930
HpyCH4IV	ACGT	2 cut(s)	1240, 1399
HpyF10VI	GCNNNNNNNGC	5 cut(s)	197, 989, 1235, 1748, 1874
HpyF3I	CTNAG	6 cut(s)	281, 492, 553, 1230, 1491, 2016
HpySE526I	ACGT	2 cut(s)	1240, 1399
Hsp92I	GRCGYC	1 cut(s)	1886
Hsp92II	CATG	9 cut(s)	15, 482, 733, 821, 973, 1302, 1357, 1945, 2078
Ksp22I	TGATCA	2 cut(s)	250, 1051
Kzo9I	GATC	8 cut(s)	17, 136, 250, 502, 715, 935, 1051, 1570
LguI	GCTCTTC	2 cut(s)	270, 2041
LmnI	GCTCC	7 cut(s)	223, 769, 1205, 1390, 1564, 1969, 2067
Lsp1109I	GCAGC	4 cut(s)	187, 649, 793, 1420
LweI	GCATC	2 cut(s)	1264, 1887
MaeI	CTAG	6 cut(s)	113, 356, 944, 977, 1857, 2067
MaeII	ACGT	2 cut(s)	1240, 1399
MaeIII	GTNAC	2 cut(s)	326, 1619
MalI	GATC	8 cut(s)	19, 138, 252, 504, 717, 937, 1053, 1572
MboI	GATC	8 cut(s)	17, 136, 250, 502, 715, 935, 1051, 1570
MfeI	CAATTG	3 cut(s)	174, 762, 993
MflI	RGATCY	1 cut(s)	715
MhlI	GDGCHC	5 cut(s)	160, 282, 1210, 1744, 2064
MlyI	GAGTC	2 cut(s)	1635, 1768
MmeI	TCCRAC	3 cut(s)	37, 867, 1841
Mph1103I	ATGCAT	1 cut(s)	1279
MroXI	GAANNNNTTC	1 cut(s)	1546
MseI	TTAA	8 cut(s)	387, 510, 687, 927, 1080, 1251, 1646, 1722
MslI	CAYNNNNRTG	2 cut(s)	477, 1635
MspA1I	CMGCKG	3 cut(s)	301, 1411, 1657
MspCI	CTTAAG	2 cut(s)	509, 926
MspI	CCGG	3 cut(s)	230, 261, 419
MspR9I	CCNGG	1 cut(s)	420
MunI	CAATTG	3 cut(s)	174, 762, 993
Mva1269I	GAATGC	1 cut(s)	705
MvnI	CGCG	2 cut(s)	1600, 1606
MwoI	GCNNNNNNNGC	5 cut(s)	197, 989, 1235, 1748, 1874
NciI	CCSGG	1 cut(s)	420
NdeI	CATATG	1 cut(s)	1330
NdeII	GATC	8 cut(s)	17, 136, 250, 502, 715, 935, 1051, 1570
NlaIII	CATG	9 cut(s)	15, 482, 733, 821, 973, 1302, 1357, 1945, 2078
NlaIV	GGNNCC	4 cut(s)	1035, 1415, 1509, 1741
NmuCI	GTSAC	2 cut(s)	326, 1619
NsiI	ATGCAT	1 cut(s)	1279
NspI	RCATGY	3 cut(s)	15, 482, 2078
PaeR7I	CTCGAG	1 cut(s)	1160
PciI	ACATGT	2 cut(s)	11, 2074
PciSI	GCTCTTC	2 cut(s)	270, 2041
PctI	GAATGC	1 cut(s)	705
PdmI	GAANNNNTTC	1 cut(s)	1546
PfeI	GAWTC	5 cut(s)	182, 611, 1019, 1426, 2011
PflFI	GACNNNGTC	1 cut(s)	1625
PflMI	CCANNNNNTGG	1 cut(s)	1299
PinAI	ACCGGT	1 cut(s)	229
PkrI	GCNGC	4 cut(s)	202, 664, 808, 1410
PleI	GAGTC	2 cut(s)	1634, 1768
PmaCI	CACGTG	1 cut(s)	1241
PmlI	CACGTG	1 cut(s)	1241
PpsI	GAGTC	2 cut(s)	1634, 1768
Ppu21I	YACGTR	1 cut(s)	1241
PscI	ACATGT	2 cut(s)	11, 2074
PshBI	ATTAAT	1 cut(s)	387
Psp124BI	GAGCTC	2 cut(s)	282, 1210
PspCI	CACGTG	1 cut(s)	1241
PspEI	GGTNACC	1 cut(s)	1619
PspN4I	GGNNCC	4 cut(s)	1035, 1415, 1509, 1741
PspPI	GGNCC	6 cut(s)	523, 973, 1033, 1414, 1508, 2002
PspXI	VCTCGAGB	1 cut(s)	1160
PsuI	RGATCY	1 cut(s)	715
PsyI	GACNNNGTC	1 cut(s)	1625
PvuII	CAGCTG	2 cut(s)	301, 1657
RsaI	GTAC	4 cut(s)	234, 535, 1594, 2030
RsaNI	GTAC	4 cut(s)	233, 534, 1593, 2029
RseI	CAYNNNNRTG	2 cut(s)	477, 1635
SacI	GAGCTC	2 cut(s)	282, 1210
SapI	GCTCTTC	2 cut(s)	270, 2041
SaqAI	TTAA	8 cut(s)	387, 510, 687, 927, 1080, 1251, 1646, 1722
SatI	GCNGC	4 cut(s)	201, 663, 807, 1409
Sau3AI	GATC	8 cut(s)	17, 136, 250, 502, 715, 935, 1051, 1570
Sau96I	GGNCC	6 cut(s)	523, 973, 1033, 1414, 1508, 2002
ScaI	AGTACT	1 cut(s)	2030
SchI	GAGTC	2 cut(s)	1635, 1768
ScrFI	CCNGG	1 cut(s)	420
SduI	GDGCHC	5 cut(s)	160, 282, 1210, 1744, 2064
SfaNI	GCATC	2 cut(s)	1264, 1887
SfcI	CTRYAG	2 cut(s)	955, 1014
Sfr274I	CTCGAG	1 cut(s)	1160
SinI	GGWCC	6 cut(s)	523, 973, 1033, 1414, 1508, 2002
SlaI	CTCGAG	1 cut(s)	1160
SmiMI	CAYNNNNRTG	2 cut(s)	477, 1635
SmlI	CTYRAG	6 cut(s)	92, 98, 509, 926, 1160, 1220
SmoI	CTYRAG	6 cut(s)	92, 98, 509, 926, 1160, 1220
SsiI	CCGC	4 cut(s)	679, 1411, 1598, 1604
SspMI	CTAG	6 cut(s)	113, 356, 944, 977, 1857, 2067
SstI	GAGCTC	2 cut(s)	282, 1210
StyD4I	CCNGG	1 cut(s)	418
StyI	CCWWGG	1 cut(s)	1890
TaaI	ACNGT	4 cut(s)	246, 407, 1864, 1930
TaiI	ACGT	2 cut(s)	1243, 1402
TaqI	TCGA	4 cut(s)	1161, 1402, 1484, 2037
TaqII	GACCGA	1 cut(s)	1638
TatI	WGTACW	2 cut(s)	533, 2028
TfiI	GAWTC	5 cut(s)	182, 611, 1019, 1426, 2011
Tru1I	TTAA	8 cut(s)	387, 510, 687, 927, 1080, 1251, 1646, 1722
Tru9I	TTAA	8 cut(s)	387, 510, 687, 927, 1080, 1251, 1646, 1722
TseFI	GTSAC	2 cut(s)	326, 1619
TseI	GCWGC	4 cut(s)	200, 662, 806, 1408
Tsp45I	GTSAC	2 cut(s)	326, 1619
TspDTI	ATGAA	8 cut(s)	66, 326, 444, 584, 926, 1194, 1568, 1662
TspGWI	ACGGA	2 cut(s)	382, 1573
Tth111I	GACNNNGTC	1 cut(s)	1625
Van91I	CCANNNNNTGG	1 cut(s)	1299
Vha464I	CTTAAG	2 cut(s)	509, 926
VpaK11BI	GGWCC	6 cut(s)	523, 973, 1033, 1414, 1508, 2002
VspI	ATTAAT	1 cut(s)	387
XapI	RAATTY	1 cut(s)	372
XbaI	TCTAGA	2 cut(s)	112, 355
XceI	RCATGY	3 cut(s)	15, 482, 2078
XhoI	CTCGAG	1 cut(s)	1160
XmiI	GTMKAC	3 cut(s)	120, 1367, 1690
XmnI	GAANNNNTTC	1 cut(s)	1546
XspI	CTAG	6 cut(s)	113, 356, 944, 977, 1857, 2067
ZrmI	AGTACT	1 cut(s)	2030
Zsp2I	ATGCAT	1 cut(s)	1279

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.