Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0009500

Physical Location & Seq

Genomic Coordinates Reverse (-)

63220978 .. 63225791

Gene Structure 4814 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G035450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2091 bp

ATGTCTAATCTCCAAAGCGAGCAGCTTCTGCAAATGGAAACAACATGCGGGTCTCTTCTATATGAGCTTCAGATAATTTGGAATGAAGTTGGAGAGACGGACACTGATAGGGACAAGATGTTGCTTGAGCTTGAGCAGGAGTGCCTTGAAGTATACAGAAGAAAGGTTGATCAGGCTAATCGGAGTAGGGCACAGCTACGTCAGGCAATTGCTGACTCTGAAGCAGAACTTGCAGCAATCTGTTCTGCAATGGGGGAGCGACCAGTTCATATTAGGCAGTCAGATCAAAATGCTGGAAGCTTGAAGGAAGAACTCAGTAAAATAATTCCACAGCTGGAGGAAATGCAGAAGAGAAAAATTGAAAGAAAAAATCAATTTCTAGAAGTTGTGGAGGAGATACAAATTATTTCAAGTGAAATTAATGGAAGCACAAGTTGCATTTCTTCTAAAATAGTTGTGGATGAAACTGATTTGTCTTTGAGAAGGCTTGAAGAATTGCACAGACAGCTCCATGCACTCCAAACAGAGAAGAGTGATAGGCTGAAGCAGATACAGGCCCATCTGGGTACATTGAACTTGCTTTGCTTAGTTCTTGGTATGGATTTCAAACAGACAATCAGTGAGGTTCATCCCAGTCTAGATAATTATGAAGGATGTAAAGATATAAGTTTGGACTTAATTGAGCGATTGGCCACTGCAATAAAGAAATTGCGCGATGTTAAATTACAGAGAATGCAAAGGCTTCAAGATCTTGCAACCACCATGTTGGAGCTGTGGAACTTGATGGATACGCCAATAGAGGAGCAACAGATGTTTCAGAATATAACATGTAACATAGCTGCTTCAGAACATGAGATAACTGAGCCAGATGCGCTCTCCGTGGACTTCATCAATTATGTTGAGGCAGAAGTGTCTCGGTTGGAAGAGTTGAAATCAAGTAAAATGAAAGAGCTTGTTCTTAAGAAGAGGACAGAGCTAGAAGAGATCTGTAGGAAGACTCATATGGTCATAGAGGCAGATAGTGCAATTGAATATGCCATTGAAGCTATAGAATCTGGAGTTGTGGACCCTTCCTGCGTATTGGACCAAATTGAGGTTCAGGTTGCAAAGGTTAAAGAGGAAGCTTTTAGCAGGAAAGACATACTTGAAAGAGTTGACAAATGGTTGTTTGCTTGTGATGAGGAGTGCTGGCTTGAGGAATATAACAGGGATGAAAACCGATATAATGCCGGCAGAGGAGCTCATCTTACTCTCAAGCGCGCTGAGAAAGCACGTGCTTTGGTTAACAAACTTCCAGCAATGGTGGATGCATTGGCTTCAAAAACCATCGCATGGGAGAAGGAGAGAGGAATTGAATTTACTTATGATGGTGTTCGTCTTCTTTCCATGCTGGAAGAGTATACTATATTACGGGATGAAAAGGAACAAGAACGCCGAAGGCAGCGGGACCAGAAAAAGATTCAGGGTCAGCTGATAGCTGAACAAGAGGCACTTTATGGGTCAAAACCTAGTCCTTCAAAGCCGAGTGTAAAAAAGGGTCCCAGAATGTCAACAGGAGGTGCAAGCAATAGAAGACTTTCAATGCAAGCTCCCAAAGCTGATCCAATTCACTCCGCTAGAGTGCCTGCTACTCCTCTGTCACGTCCTAGTTCAAGGAAGAGTGATCGAGGCCACCAAAATGATCATCACGATGATGGCCTTACAGCTTTGGCCACTGGAAGGAGAACATTAAGTCGGAATAGCTCTTTTGGTTCCAACTATGAACCAGAGTCACCCATGCGGAAACCCTTTGCGCCAATTTCTCTTCCTACTGACAACGGCACATTTGCTACTCCTATGAAGTCCACAAATGTTCCAGAGGAAGACAACACAAAAACACCCAAGGTGATGACTTTTGTGCCCACCACACCACAAACAGTGTCGGTACCAATGCAGACAGCCGTGACCCCAGCTCCTCCACCACCTCAGCCGATTCCTTATGCCACCAAAGTAGTCGAGGAGGTTACTCCTGAAGAGATTGAATACTCTTTTGAGGAAATCAGAGCGGGTTTCGTGCTCCCTGAAGCACATACCAAGTCGATACATGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0008017 GO:0008092 GO:0008150 GO:0009524 GO:0009574 GO:0009605 GO:0009607 GO:0009624 GO:0009653 GO:0009987 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0032502 GO:0032506 GO:0034622 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043933 GO:0044002 GO:0044085 GO:0044111 GO:0044115 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0046785 GO:0048285 GO:0048646 GO:0048856 GO:0050896 GO:0051258 GO:0051301 GO:0051701 GO:0051704 GO:0051707 GO:0051816 GO:0052093 GO:0052095 GO:0052096 GO:0055028 GO:0061640 GO:0065003 GO:0071840 GO:0071944 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MAP65_ASE1

Protein Analysis

696

Amino Acids

78.97

Weight (kDa)

5.34

Isoelectric Point (pI)

59.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MAP65_ASE1	PF03999	18 - 508	1.9e-115	Microtubule associated protein (MAP65/ASE1 family)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009500)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51600
fragaria_vesca	FvH4_7g28190 FvH4_7g28190 FvH4_7g28190 FvH4_7g28190
malus_domestica	MD01G1185800.v1.1 MD07G1257900.v1.1
prunus_persica	Prupe.2G282400_v2.0.a1 Prupe.2G282400_v2.0.a1 Prupe.2G282400_v2.0.a1
pyrus_communis	pycom01g19750 pycom07g23100
rosa_chinensis	RchiOBHm_Chr1g0375141
rosa_laevigata	RLG00000026683
rosa_multiflora	Rmu_sc0000554.1_g000022
rosa_roxburghii	Rroxscaffold_4G00282980
rosa_rugosa	Rorug01G0388800
rosa_samantha	Rh1AG399300 Rh1BG365200 Rh1CG378100 Rh1DG395900
rosa_wichuraiana	Rw1G035450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1924
AccB1I	GGYRCC	1 cut(s)	1924
AccB7I	CCANNNNNTGG	1 cut(s)	1332
AccBSI	CCGCTC	1 cut(s)	2045
AccI	GTMKAC	2 cut(s)	153, 1400
AccII	CGCG	2 cut(s)	714, 1260
AciI	CCGC	5 cut(s)	48, 1444, 1614, 1780, 2045
AclWI	GGATC	1 cut(s)	1595
AcoI	YGGCCR	2 cut(s)	690, 1710
AcsI	RAATTY	1 cut(s)	1355
AcuI	CTGAAG	6 cut(s)	53, 240, 563, 828, 2031, 2082
AcvI	CACGTG	1 cut(s)	1274
AfaI	GTAC	2 cut(s)	568, 1926
AfiI	CCNNNNNNNGG	3 cut(s)	1093, 1332, 1719
AflII	CTTAAG	1 cut(s)	959
AflIII	ACRYGT	2 cut(s)	827, 2083
AjiI	CACGTC	1 cut(s)	1643
AjuI	GAANNNNNNNTTGG	2 cut(s)	1080, 1112
Alw21I	GWGCWC	2 cut(s)	1243, 2058
Alw26I	GTCTC	3 cut(s)	57, 89, 918
AlwI	GGATC	1 cut(s)	1595
AlwNI	CAGNNNCTG	1 cut(s)	28
AoxI	GGCC	5 cut(s)	555, 690, 1669, 1696, 1710
ApeKI	GCWGC	4 cut(s)	22, 233, 839, 1441
ApoI	RAATTY	1 cut(s)	1355
AseI	ATTAAT	1 cut(s)	420
Asp700I	GAANNNNTTC	1 cut(s)	1576
Asp718I	GGTACC	1 cut(s)	1924
AspLEI	GCGC	5 cut(s)	714, 874, 1260, 1262, 1795
AspS9I	GGNCC	5 cut(s)	556, 1066, 1084, 1447, 1538
AsuHPI	GGTGA	2 cut(s)	1764, 1897
AvaII	GGWCC	4 cut(s)	1066, 1084, 1447, 1538
BaeGI	GKGCMC	2 cut(s)	193, 1902
BalI	TGGCCA	2 cut(s)	692, 1712
BanI	GGYRCC	1 cut(s)	1924
BanII	GRGCYC	1 cut(s)	1243
BbrPI	CACGTG	1 cut(s)	1274
BbsI	GAAGAC	4 cut(s)	1001, 1370, 1579, 1869
Bbv12I	GWGCWC	2 cut(s)	1243, 2058
BbvCI	CCTCAGC	1 cut(s)	1965
BbvI	GCAGC	4 cut(s)	34, 245, 826, 1453
BccI	CCATC	5 cut(s)	567, 778, 1334, 1361, 1688
BceAI	ACGGC	2 cut(s)	1834, 1925
BciVI	GTATCC	1 cut(s)	781
BclI	TGATCA	2 cut(s)	169, 1681
BcoDI	GTCTC	3 cut(s)	57, 89, 918
BfaI	CTAG	6 cut(s)	380, 638, 977, 1509, 1617, 1647
BfmI	CTRYAG	2 cut(s)	988, 1047
BfrI	CTTAAG	1 cut(s)	959
BfuI	GTATCC	1 cut(s)	781
BglII	AGATCT	2 cut(s)	748, 984
BisI	GCNGC	4 cut(s)	23, 234, 840, 1442
BlsI	GCNGC	4 cut(s)	24, 235, 841, 1443
Bme18I	GGWCC	4 cut(s)	1066, 1084, 1447, 1538
BmgBI	CACGTC	1 cut(s)	1643
BmgT120I	GGNCC	5 cut(s)	556, 1066, 1084, 1447, 1538
BmiI	GGNNCC	6 cut(s)	1068, 1448, 1539, 1540, 1753, 1926
BmrI	ACTGGG	1 cut(s)	627
BmsI	GCATC	2 cut(s)	859, 1297
BmuI	ACTGGG	1 cut(s)	627
BpiI	GAAGAC	4 cut(s)	1001, 1370, 1579, 1869
BpmI	CTGGAG	2 cut(s)	356, 1077
Bpu10I	CCTNAGC	1 cut(s)	1965
BpuEI	CTTGAG	4 cut(s)	146, 152, 1214, 1238
BsaAI	YACGTR	1 cut(s)	1274
BsaI	GGTCTC	1 cut(s)	57
BsaJI	CCNNGG	2 cut(s)	879, 1881
BsaXI	ACNNNNNCTCC	2 cut(s)	1936, 1966
Bsc4I	CCNNNNNNNGG	3 cut(s)	1093, 1332, 1719
Bse118I	RCCGGY	1 cut(s)	1229
Bse1I	ACTGG	3 cut(s)	263, 633, 1720
Bse3DI	GCAATG	2 cut(s)	255, 1305
BseDI	CCNNGG	2 cut(s)	879, 1881
BseGI	GGATG	6 cut(s)	466, 628, 659, 1216, 1312, 1420
BseLI	CCNNNNNNNGG	3 cut(s)	1093, 1332, 1719
BseMI	GCAATG	2 cut(s)	255, 1305
BseMII	CTCAG	4 cut(s)	328, 852, 1254, 1979
BseNI	ACTGG	3 cut(s)	263, 633, 1720
BsePI	GCGCGC	1 cut(s)	1258
BseRI	GAGGAG	7 cut(s)	407, 815, 1196, 1251, 1623, 1944, 2012
BseSI	GKGCMC	2 cut(s)	193, 1902
BseXI	GCAGC	4 cut(s)	34, 245, 826, 1453
BseYI	CCCAGC	1 cut(s)	1948
Bsh1236I	CGCG	2 cut(s)	714, 1260
BshFI	GGCC	5 cut(s)	557, 692, 1671, 1698, 1712
BshNI	GGYRCC	1 cut(s)	1924
BsiHKAI	GWGCWC	2 cut(s)	1243, 2058
BsiSI	CCGG	1 cut(s)	1230
BslFI	GGGAC	3 cut(s)	125, 1460, 1524
BslI	CCNNNNNNNGG	3 cut(s)	1093, 1332, 1719
BsmAI	GTCTC	3 cut(s)	57, 89, 918
BsmBI	CGTCTC	1 cut(s)	89
BsmFI	GGGAC	3 cut(s)	125, 1460, 1524
BsmI	GAATGC	1 cut(s)	738
BsnI	GGCC	5 cut(s)	557, 692, 1671, 1698, 1712
Bso31I	GGTCTC	1 cut(s)	57
Bsp1286I	GDGCHC	4 cut(s)	193, 1243, 1902, 2058
Bsp143I	GATC	7 cut(s)	169, 283, 748, 984, 1600, 1663, 1681
BspACI	CCGC	5 cut(s)	48, 1444, 1614, 1780, 2045
BspANI	GGCC	5 cut(s)	557, 692, 1671, 1698, 1712
BspCNI	CTCAG	4 cut(s)	327, 853, 1255, 1978
BspFNI	CGCG	2 cut(s)	714, 1260
BspLI	GGNNCC	6 cut(s)	1068, 1448, 1539, 1540, 1753, 1926
BspPI	GGATC	1 cut(s)	1595
BspT107I	GGYRCC	1 cut(s)	1924
BspTI	CTTAAG	1 cut(s)	959
BspTNI	GGTCTC	1 cut(s)	57
BsrBI	CCGCTC	1 cut(s)	2045
BsrDI	GCAATG	2 cut(s)	255, 1305
BsrFI	RCCGGY	1 cut(s)	1229
BsrI	ACTGG	3 cut(s)	263, 633, 1720
BssAI	RCCGGY	1 cut(s)	1229
BssECI	CCNNGG	2 cut(s)	879, 1881
BssHII	GCGCGC	1 cut(s)	1258
BssMI	GATC	7 cut(s)	169, 283, 748, 984, 1600, 1663, 1681
BssNAI	GTATAC	2 cut(s)	154, 1401
BssT1I	CCWWGG	1 cut(s)	1881
Bst1107I	GTATAC	2 cut(s)	154, 1401
Bst4CI	ACNGT	1 cut(s)	1918
BstAFI	CTTAAG	1 cut(s)	959
BstAPI	GCANNNNNTGC	4 cut(s)	28, 230, 435, 1022
BstBAI	YACGTR	1 cut(s)	1274
BstC8I	GCNNGC	7 cut(s)	20, 1190, 1231, 1260, 1564, 1587, 1626
BstDEI	CTNAG	5 cut(s)	314, 586, 861, 1263, 1965
BstDSI	CCRYGG	1 cut(s)	879
BstF5I	GGATG	6 cut(s)	466, 628, 659, 1216, 1312, 1420
BstFNI	CGCG	2 cut(s)	714, 1260
BstHHI	GCGC	5 cut(s)	714, 874, 1260, 1262, 1795
BstKTI	GATC	7 cut(s)	172, 286, 751, 987, 1603, 1666, 1684
BstMAI	GTCTC	3 cut(s)	57, 89, 918
BstMBI	GATC	7 cut(s)	169, 283, 748, 984, 1600, 1663, 1681
BstMWI	GCNNNNNNNGC	9 cut(s)	28, 230, 435, 505, 871, 1022, 1043, 1268, 1595
BstNSI	RCATGY	3 cut(s)	48, 831, 2087
BstSFI	CTRYAG	2 cut(s)	988, 1047
BstSLI	GKGCMC	2 cut(s)	193, 1902
BstUI	CGCG	2 cut(s)	714, 1260
BstV1I	GCAGC	4 cut(s)	34, 245, 826, 1453
BstV2I	GAAGAC	4 cut(s)	1001, 1370, 1579, 1869
BstX2I	RGATCY	2 cut(s)	748, 984
BstXI	CCANNNNNNTGG	1 cut(s)	766
BstYI	RGATCY	2 cut(s)	748, 984
BstZ17I	GTATAC	2 cut(s)	154, 1401
BsuI	GTATCC	1 cut(s)	781
BsuRI	GGCC	5 cut(s)	557, 692, 1671, 1698, 1712
BtgI	CCRYGG	1 cut(s)	879
BtgZI	GCGATG	2 cut(s)	729, 1312
BtrI	CACGTC	1 cut(s)	1643
BtsCI	GGATG	6 cut(s)	466, 628, 659, 1216, 1312, 1420
BtsI	GCAGTG	1 cut(s)	693
BtsIMutI	CAGTG	5 cut(s)	102, 625, 693, 1713, 1923
Cac8I	GCNNGC	7 cut(s)	20, 1190, 1231, 1260, 1564, 1587, 1626
CaiI	CAGNNNCTG	1 cut(s)	28
CfoI	GCGC	5 cut(s)	714, 874, 1260, 1262, 1795
Cfr10I	RCCGGY	1 cut(s)	1229
Cfr13I	GGNCC	5 cut(s)	556, 1066, 1084, 1447, 1538
Csp6I	GTAC	2 cut(s)	567, 1925
CviAII	CATG	9 cut(s)	45, 512, 763, 828, 851, 1332, 1387, 1777, 2084
CviQI	GTAC	2 cut(s)	567, 1925
DdeI	CTNAG	5 cut(s)	314, 586, 861, 1263, 1965
DpnI	GATC	7 cut(s)	171, 285, 750, 986, 1602, 1665, 1683
DpnII	GATC	7 cut(s)	169, 283, 748, 984, 1600, 1663, 1681
EaeI	YGGCCR	2 cut(s)	690, 1710
Ecl136II	GAGCTC	1 cut(s)	1241
Eco130I	CCWWGG	1 cut(s)	1881
Eco24I	GRGCYC	1 cut(s)	1243
Eco31I	GGTCTC	1 cut(s)	57
Eco47I	GGWCC	4 cut(s)	1066, 1084, 1447, 1538
Eco53kI	GAGCTC	1 cut(s)	1241
Eco57I	CTGAAG	6 cut(s)	53, 240, 563, 828, 2031, 2082
Eco72I	CACGTG	1 cut(s)	1274
EcoICRI	GAGCTC	1 cut(s)	1241
EcoO109I	RGGNCCY	1 cut(s)	1538
EcoT14I	CCWWGG	1 cut(s)	1881
EcoT22I	ATGCAT	1 cut(s)	1312
EcoT38I	GRGCYC	1 cut(s)	1243
ErhI	CCWWGG	1 cut(s)	1881
Esp3I	CGTCTC	1 cut(s)	89
FaeI	CATG	9 cut(s)	48, 515, 766, 831, 854, 1335, 1390, 1780, 2087
FalI	AAGNNNNNCTT	6 cut(s)	213, 245, 1128, 1160, 1476, 1508
FaqI	GGGAC	3 cut(s)	125, 1460, 1524
FatI	CATG	9 cut(s)	44, 511, 762, 827, 850, 1331, 1386, 1776, 2083
FauI	CCCGC	3 cut(s)	41, 1437, 2038
FauNDI	CATATG	1 cut(s)	1002
FbaI	TGATCA	2 cut(s)	169, 1681
FblI	GTMKAC	2 cut(s)	153, 1400
Fnu4HI	GCNGC	4 cut(s)	23, 234, 840, 1442
FokI	GGATG	6 cut(s)	473, 615, 666, 1223, 1319, 1427
FriOI	GRGCYC	1 cut(s)	1243
Fsp4HI	GCNGC	4 cut(s)	23, 234, 840, 1442
FspBI	CTAG	6 cut(s)	380, 638, 977, 1509, 1617, 1647
GlaI	GCGC	5 cut(s)	713, 873, 1259, 1261, 1794
GluI	GCNGC	4 cut(s)	23, 234, 840, 1442
GsaI	CCCAGC	1 cut(s)	1952
GsuI	CTGGAG	2 cut(s)	356, 1077
HaeIII	GGCC	5 cut(s)	557, 692, 1671, 1698, 1712
HapII	CCGG	1 cut(s)	1230
HhaI	GCGC	5 cut(s)	714, 874, 1260, 1262, 1795
Hin1II	CATG	9 cut(s)	48, 515, 766, 831, 854, 1335, 1390, 1780, 2087
Hin6I	GCGC	5 cut(s)	712, 872, 1258, 1260, 1793
HinP1I	GCGC	5 cut(s)	712, 872, 1258, 1260, 1793
HincII	GTYRAC	3 cut(s)	1156, 1285, 1551
HindII	GTYRAC	3 cut(s)	1156, 1285, 1551
HindIII	AAGCTT	2 cut(s)	298, 1122
HinfI	GANTC	6 cut(s)	215, 997, 1052, 1459, 1769, 1972
HpaI	GTTAAC	1 cut(s)	1285
HpaII	CCGG	1 cut(s)	1230
HphI	GGTGA	2 cut(s)	1764, 1897
Hpy166II	GTNNAC	8 cut(s)	154, 883, 1066, 1156, 1285, 1401, 1551, 1845
Hpy188I	TCNGA	9 cut(s)	72, 183, 220, 283, 819, 847, 1737, 2042, 2090
Hpy188III	TCNNGA	7 cut(s)	380, 638, 746, 1056, 1688, 1856, 2009
Hpy8I	GTNNAC	8 cut(s)	154, 883, 1066, 1156, 1285, 1401, 1551, 1845
HpyAV	CCTTC	8 cut(s)	298, 477, 644, 1080, 1333, 1431, 1524, 1713
HpyCH4III	ACNGT	1 cut(s)	1918
HpyCH4IV	ACGT	3 cut(s)	199, 1273, 1642
HpyF10VI	GCNNNNNNNGC	9 cut(s)	28, 230, 435, 505, 871, 1022, 1043, 1268, 1595
HpyF3I	CTNAG	5 cut(s)	314, 586, 861, 1263, 1965
HpySE526I	ACGT	3 cut(s)	199, 1273, 1642
Hsp92II	CATG	9 cut(s)	48, 515, 766, 831, 854, 1335, 1390, 1780, 2087
HspAI	GCGC	5 cut(s)	712, 872, 1258, 1260, 1793
KflI	GGGWCCC	1 cut(s)	1538
KpnI	GGTACC	1 cut(s)	1928
KroI	GCCGGC	1 cut(s)	1229
KroNI	GCCGGC	1 cut(s)	1231
Ksp22I	TGATCA	2 cut(s)	169, 1681
KspAI	GTTAAC	1 cut(s)	1285
Kzo9I	GATC	7 cut(s)	169, 283, 748, 984, 1600, 1663, 1681
LmnI	GCTCC	8 cut(s)	256, 513, 769, 802, 1238, 1594, 1957, 2061
Lsp1109I	GCAGC	4 cut(s)	34, 245, 826, 1453
LweI	GCATC	2 cut(s)	859, 1297
MaeI	CTAG	6 cut(s)	380, 638, 977, 1509, 1617, 1647
MaeII	ACGT	3 cut(s)	199, 1273, 1642
MaeIII	GTNAC	5 cut(s)	830, 1638, 1770, 1942, 2002
MalI	GATC	7 cut(s)	171, 285, 750, 986, 1602, 1665, 1683
MbiI	CCGCTC	1 cut(s)	2045
MboI	GATC	7 cut(s)	169, 283, 748, 984, 1600, 1663, 1681
MfeI	CAATTG	2 cut(s)	207, 1026
MflI	RGATCY	2 cut(s)	748, 984
MhlI	GDGCHC	4 cut(s)	193, 1243, 1902, 2058
MlsI	TGGCCA	2 cut(s)	692, 1712
MluNI	TGGCCA	2 cut(s)	692, 1712
MlyI	GAGTC	3 cut(s)	209, 991, 1778
MmeI	TCCRAC	5 cut(s)	70, 747, 900, 1715, 1779
Mox20I	TGGCCA	2 cut(s)	692, 1712
Mph1103I	ATGCAT	1 cut(s)	1312
MroNI	GCCGGC	1 cut(s)	1229
MroXI	GAANNNNTTC	1 cut(s)	1576
MscI	TGGCCA	2 cut(s)	692, 1712
MseI	TTAA	7 cut(s)	420, 677, 720, 960, 1113, 1284, 1730
MslI	CAYNNNNRTG	3 cut(s)	1677, 1689, 1692
Msp20I	TGGCCA	2 cut(s)	692, 1712
MspA1I	CMGCKG	3 cut(s)	334, 1444, 1471
MspCI	CTTAAG	1 cut(s)	959
MspI	CCGG	1 cut(s)	1230
MunI	CAATTG	2 cut(s)	207, 1026
Mva1269I	GAATGC	1 cut(s)	738
MvnI	CGCG	2 cut(s)	714, 1260
MwoI	GCNNNNNNNGC	9 cut(s)	28, 230, 435, 505, 871, 1022, 1043, 1268, 1595
NaeI	GCCGGC	1 cut(s)	1231
NdeI	CATATG	1 cut(s)	1002
NdeII	GATC	7 cut(s)	169, 283, 748, 984, 1600, 1663, 1681
NgoMIV	GCCGGC	1 cut(s)	1229
NlaIII	CATG	9 cut(s)	48, 515, 766, 831, 854, 1335, 1390, 1780, 2087
NlaIV	GGNNCC	6 cut(s)	1068, 1448, 1539, 1540, 1753, 1926
NmeAIII	GCCGAG	1 cut(s)	1548
NmuCI	GTSAC	3 cut(s)	1638, 1770, 1942
NsiI	ATGCAT	1 cut(s)	1312
NspI	RCATGY	3 cut(s)	48, 831, 2087
PauI	GCGCGC	1 cut(s)	1258
PciI	ACATGT	2 cut(s)	827, 2083
PctI	GAATGC	1 cut(s)	738
PdiI	GCCGGC	1 cut(s)	1231
PdmI	GAANNNNTTC	1 cut(s)	1576
PfeI	GAWTC	3 cut(s)	1052, 1459, 1972
PflMI	CCANNNNNTGG	1 cut(s)	1332
PkrI	GCNGC	4 cut(s)	24, 235, 841, 1443
PleI	GAGTC	3 cut(s)	209, 991, 1777
PmaCI	CACGTG	1 cut(s)	1274
PmlI	CACGTG	1 cut(s)	1274
PpsI	GAGTC	3 cut(s)	209, 991, 1777
Ppu21I	YACGTR	1 cut(s)	1274
PpuMI	RGGWCCY	1 cut(s)	1538
PscI	ACATGT	2 cut(s)	827, 2083
PshBI	ATTAAT	1 cut(s)	420
Psp124BI	GAGCTC	1 cut(s)	1243
Psp5II	RGGWCCY	1 cut(s)	1538
PspCI	CACGTG	1 cut(s)	1274
PspFI	CCCAGC	1 cut(s)	1948
PspN4I	GGNNCC	6 cut(s)	1068, 1448, 1539, 1540, 1753, 1926
PspPI	GGNCC	5 cut(s)	556, 1066, 1084, 1447, 1538
PspPPI	RGGWCCY	1 cut(s)	1538
PstNI	CAGNNNCTG	1 cut(s)	28
PsuI	RGATCY	2 cut(s)	748, 984
PteI	GCGCGC	1 cut(s)	1258
PvuII	CAGCTG	2 cut(s)	334, 1471
RsaI	GTAC	2 cut(s)	568, 1926
RsaNI	GTAC	2 cut(s)	567, 1925
RseI	CAYNNNNRTG	3 cut(s)	1677, 1689, 1692
SacI	GAGCTC	1 cut(s)	1243
SaqAI	TTAA	7 cut(s)	420, 677, 720, 960, 1113, 1284, 1730
SatI	GCNGC	4 cut(s)	23, 234, 840, 1442
Sau3AI	GATC	7 cut(s)	169, 283, 748, 984, 1600, 1663, 1681
Sau96I	GGNCC	5 cut(s)	556, 1066, 1084, 1447, 1538
SchI	GAGTC	3 cut(s)	209, 991, 1778
SduI	GDGCHC	4 cut(s)	193, 1243, 1902, 2058
SfaNI	GCATC	2 cut(s)	859, 1297
SfcI	CTRYAG	2 cut(s)	988, 1047
SinI	GGWCC	4 cut(s)	1066, 1084, 1447, 1538
SmiMI	CAYNNNNRTG	3 cut(s)	1677, 1689, 1692
SmlI	CTYRAG	5 cut(s)	125, 131, 959, 1193, 1253
SmoI	CTYRAG	5 cut(s)	125, 131, 959, 1193, 1253
SsiI	CCGC	5 cut(s)	48, 1444, 1614, 1780, 2045
SspMI	CTAG	6 cut(s)	380, 638, 977, 1509, 1617, 1647
SstI	GAGCTC	1 cut(s)	1243
StyI	CCWWGG	1 cut(s)	1881
TaaI	ACNGT	1 cut(s)	1918
TaiI	ACGT	3 cut(s)	202, 1276, 1645
TaqI	TCGA	3 cut(s)	1666, 1995, 2078
TfiI	GAWTC	3 cut(s)	1052, 1459, 1972
Tru1I	TTAA	7 cut(s)	420, 677, 720, 960, 1113, 1284, 1730
Tru9I	TTAA	7 cut(s)	420, 677, 720, 960, 1113, 1284, 1730
TscAI	CASTG	5 cut(s)	109, 625, 700, 1720, 1923
TseFI	GTSAC	3 cut(s)	1638, 1770, 1942
TseI	GCWGC	4 cut(s)	22, 233, 839, 1441
Tsp45I	GTSAC	3 cut(s)	1638, 1770, 1942
TspGWI	ACGGA	2 cut(s)	113, 868
TspRI	CASTG	5 cut(s)	109, 625, 700, 1720, 1923
Van91I	CCANNNNNTGG	1 cut(s)	1332
Vha464I	CTTAAG	1 cut(s)	959
VpaK11BI	GGWCC	4 cut(s)	1066, 1084, 1447, 1538
VspI	ATTAAT	1 cut(s)	420
XapI	RAATTY	1 cut(s)	1355
XbaI	TCTAGA	2 cut(s)	379, 637
XceI	RCATGY	3 cut(s)	48, 831, 2087
XmiI	GTMKAC	2 cut(s)	153, 1400
XmnI	GAANNNNTTC	1 cut(s)	1576
XspI	CTAG	6 cut(s)	380, 638, 977, 1509, 1617, 1647
Zsp2I	ATGCAT	1 cut(s)	1312

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.