Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0008760

Physical Location & Seq

Genomic Coordinates Forward (+)

34775331 .. 34779060

Gene Structure 3730 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1285600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1902 bp

ATGTTCGAAAACCAACCAATTCCCGACTGTCGATTCTGTGGAATAATAATTTGTGGACACCGTTTTCCCCGCATATACGTTATTACTATTAAAGCTTCGAACTTTTACGTGACCGGAAAAAAATTCTGGAAAAAAATTCTGGAAAAAAAGTCTCGAACTTTCTTAAACTCTTACTCCCGAGAGTCTCCGAAGTTCTTGTCATCGGATCAGTCGAGCATTGTTTCTGCAAAAGAGGGGAAGAGCTTAAAACTATGGCTGCATTTCTTTATGTATGGTTGCAGAGTTTGTGCACAAATAAAGCTTTCTCACTCATCGCTTGGACTATTCCTGAGGCAACTTGGCATGGGAGTTGTGACAGTTTCGACCTCAGCGGCAAGGACTCCGTTAGGATTGAGCGCAAAATCAACAAGCCAGAGATCTACATTCAGAAAGCCGTTGATAGTTGCATTTAAAACAGATGAATCCAATAAGACGGCATTGGTTGCACCCCAGGGGAAAATACCTTTGCCTATAGAAACACGAAAGAAGCACCAAAAGAGAATAGGGAAAGCTAGAAAACTCGCAAAAAGTGTGAAATCTACTGCTACTGATGTAGCTGCTCCGTGTACTACTTTGGAAGTGGATTACAATGAAGCCGCTGCCAAGCTGGAAAACTTGTACAAGCGTAGCCCCGAGATTGAAGCTGATGTGGATGTGGAAGATGCAGATGGCTTGATGAGGAGGGGTCGCAAAAGGAGGAAGAAGACTAGCGAAAGCAATCATGAGAAAGAAGAGAAGAGAACAAGTGATATTGTGGTTAGAAGCAAGACAAAGAAGGCGAAAAGATTGAATCTTGATAAAAGGGTTTCATTGAAATGGAACAAGGACGAGAAAGTGGTTTCTTCAGTTCAAAAGAGGAAAGATCAAAAGAACGAAATGGAGAAGATTGAGGAGCTGGTTAGGGAGTATTCGGCTTCAACTGATCTGGTCAGTTTGGACTGGAAAAAGATGAAGATTCCTCCGGTTCTCACTTCTTCAGAACATGCTTGGTTGTTCAAGCTAATGCAACCTATGAAGGGTCTCCATGAAGCGAGAGAGCAATTACAAAAAGATTTGGGGACAGAACCAACCAACGGCGAATTGGCTGAGGCGACAAACATGAGTGTGGTTCAAGTGAAAAAACATTTCGAAGTTGGTCGAGCTGCACGAAACAAGCTCATCAAGCACAATCTCCGACTTGTGTTATTCGTCATTAATAAATATTTTCAAGACTTTGCAAATGGTCCAAAATTTCAAGACCTCTGCCAGGCAGGAGTAAGGGGGCTTATTACGGCAATTGATCGCTTTGAACCAAAAAGAAGTTTTCGGCTTTCCACATATGGTCTGTTCTGGATCAGGCATGCCATCATACGTTCCATGACAGTCTCAAGCTTTACTCGTGTTTCATTTGGCCTTGAGTCGGTTAGGGCGGAAATTCAAAAGGCCAAGCTTGAGATGATATTTTCGCTCAAAAGGATTCCAACTGACGAAGAGATAATCGAAAAAGTTGGAATTTCTCCAGAGAGGTACCATGAAGTAATGAGGGCCTCAAAACCTGTTTCCTCTCTTCATTCAAGGCATGCAACCACACAAGAGGAGTATATCAACGGTATTACTGACGTCGATGGTGTTGAAGGTGATAACCGGCGGAAACCTGCTCTTCTCAGGCTTGCTATTGATGATGTGCTTGATTCCCTGAAGCCGAAAGAAAGCCTGGTGATCAGACAGAGATACGGGCTTGATGGTAAGGGGGATAGAACATTGGGGGAGATTGCTGGAAACTTAAATATTTCACGAGAAATGGTTCGAAAGCATGAAGTCAAGGCTCTCATGAAGCTCAAACATCCAGCTCGAGTAGATTATCTTCGCAGATACATAGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000988 GO:0000990 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006091 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009553 GO:0009628 GO:0009637 GO:0009639 GO:0009651 GO:0009657 GO:0009658 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010114 GO:0010207 GO:0010218 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015979 GO:0016043 GO:0016070 GO:0016987 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019684 GO:0022607 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0046483 GO:0048229 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0071214 GO:0071470 GO:0071472 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0104004 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2000142 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

634

Amino Acids

72.32

Weight (kDa)

9.95

Isoelectric Point (pI)

40.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sigma70_r2	PF04542	399 - 465	1.9e-11	Sigma-70 region 2
Sigma70_r3	PF04539	483 - 546	4.7e-11	Sigma-70 region 3
Sigma70_r4	PF04545	568 - 621	1.3e-13	Sigma-70, region 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008760)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24120 AT5G24120 AT5G24120
fragaria_vesca	FvH4_7g31060 FvH4_7g31060
malus_domestica	MD07G1285600.v1.1
prunus_persica	Prupe.2G307500_v2.0.a1 Prupe.2G307500_v2.0.a1
pyrus_communis	pycom07g25910
rosa_chinensis	RchiOBHm_Chr1g0380131
rosa_laevigata	RLG00000026328
rosa_multiflora	Rmu_sc0001232.1_g000016
rosa_roxburghii	Rroxscaffold_4G00279650
rosa_rugosa	Rorug01G0417700 Rorug01G0417800 Rorug01G0417900 Rorug01G0418000 Rorug01G0418000
rosa_samantha	Rh1AG443000 Rh1BG397900 Rh1CG411400 Rh1DG428700
rosa_wichuraiana	Rw1G038460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1641
Acc36I	ACCTGC	1 cut(s)	1681
Acc65I	GGTACC	1 cut(s)	1545
AccB1I	GGYRCC	1 cut(s)	1545
AciI	CCGC	5 cut(s)	70, 371, 636, 1448, 1666
AclWI	GGATC	2 cut(s)	213, 1379
AcsI	RAATTY	5 cut(s)	122, 135, 1268, 1452, 1530
AcuI	CTGAAG	3 cut(s)	867, 999, 1736
AcyI	GRCGYC	1 cut(s)	1638
AfaI	GTAC	3 cut(s)	607, 659, 1547
AfiI	CCNNNNNNNGG	4 cut(s)	1055, 1112, 1285, 1438
AjnI	CCWGG	3 cut(s)	489, 1284, 1731
Alw21I	GWGCWC	1 cut(s)	292
Alw26I	GTCTC	4 cut(s)	156, 189, 1064, 1408
Alw44I	GTGCAC	1 cut(s)	288
AlwI	GGATC	2 cut(s)	213, 1379
Ama87I	CYCGRG	3 cut(s)	177, 671, 1869
AoxI	GGCC	3 cut(s)	1429, 1461, 1563
ApaLI	GTGCAC	1 cut(s)	288
ApeKI	GCWGC	4 cut(s)	256, 596, 638, 1181
ApoI	RAATTY	5 cut(s)	122, 135, 1268, 1452, 1530
ArsI	GACNNNNNNTTYG	2 cut(s)	182, 214
AseI	ATTAAT	1 cut(s)	1233
Asp700I	GAANNNNTTC	1 cut(s)	1821
Asp718I	GGTACC	1 cut(s)	1545
AspLEI	GCGC	1 cut(s)	398
AspS9I	GGNCC	2 cut(s)	1262, 1563
AsuHPI	GGTGA	2 cut(s)	1667, 1747
AsuII	TTCGAA	4 cut(s)	6, 98, 1167, 1825
AvaI	CYCGRG	3 cut(s)	177, 671, 1869
AvaII	GGWCC	1 cut(s)	1262
AxyI	CCTNAGG	1 cut(s)	329
BaeGI	GKGCMC	1 cut(s)	292
BanI	GGYRCC	1 cut(s)	1545
BarI	GAAGNNNNNNTAC	2 cut(s)	1866, 1898
BauI	CACGAG	2 cut(s)	1416, 1812
BbsI	GAAGAC	1 cut(s)	749
Bbv12I	GWGCWC	1 cut(s)	292
BbvCI	CCTCAGC	2 cut(s)	367, 1125
BbvI	GCAGC	4 cut(s)	243, 583, 625, 1168
BccI	CCATC	4 cut(s)	701, 1391, 1637, 1754
BceAI	ACGGC	4 cut(s)	418, 489, 1129, 1326
BciT130I	CCWGG	3 cut(s)	491, 1286, 1733
BclI	TGATCA	1 cut(s)	1737
BcoDI	GTCTC	4 cut(s)	156, 189, 1064, 1408
BfaI	CTAG	2 cut(s)	552, 747
BfmI	CTRYAG	1 cut(s)	510
BfuAI	ACCTGC	1 cut(s)	1681
BglII	AGATCT	1 cut(s)	416
BisI	GCNGC	6 cut(s)	257, 372, 597, 636, 639, 1182
BlsI	GCNGC	6 cut(s)	258, 373, 598, 637, 640, 1183
Bme1390I	CCNGG	3 cut(s)	491, 1286, 1733
Bme18I	GGWCC	1 cut(s)	1262
BmeT110I	CYCGRG	3 cut(s)	177, 671, 1869
BmgT120I	GGNCC	2 cut(s)	1262, 1563
BmiI	GGNNCC	1 cut(s)	1547
BmrFI	CCNGG	3 cut(s)	491, 1286, 1733
BmsI	GCATC	1 cut(s)	691
BpiI	GAAGAC	1 cut(s)	749
BpmI	CTGGAG	1 cut(s)	1521
Bpu10I	CCTNAGC	2 cut(s)	367, 1125
Bpu14I	TTCGAA	4 cut(s)	6, 98, 1167, 1825
BpuEI	CTTGAG	3 cut(s)	1390, 1454, 1490
BsaAI	YACGTR	1 cut(s)	109
BsaHI	GRCGYC	1 cut(s)	1638
BsaI	GGTCTC	1 cut(s)	1064
BsaJI	CCNNGG	2 cut(s)	489, 490
BsaWI	WCCGGW	2 cut(s)	113, 1000
BsaXI	ACNNNNNCTCC	2 cut(s)	158, 188
Bsc4I	CCNNNNNNNGG	4 cut(s)	1055, 1112, 1285, 1438
Bse118I	RCCGGY	1 cut(s)	1662
Bse1I	ACTGG	1 cut(s)	983
Bse21I	CCTNAGG	1 cut(s)	329
BseBI	CCWGG	3 cut(s)	491, 1286, 1733
BseDI	CCNNGG	2 cut(s)	489, 490
BseGI	GGATG	2 cut(s)	697, 1861
BseLI	CCNNNNNNNGG	4 cut(s)	1055, 1112, 1285, 1438
BseMII	CTCAG	4 cut(s)	320, 381, 1116, 1696
BseNI	ACTGG	1 cut(s)	983
BseRI	GAGGAG	3 cut(s)	733, 944, 1628
BseSI	GKGCMC	1 cut(s)	292
BseXI	GCAGC	4 cut(s)	243, 583, 625, 1168
BsgI	GTGCAG	1 cut(s)	1167
BshFI	GGCC	3 cut(s)	1431, 1463, 1565
BshNI	GGYRCC	1 cut(s)	1545
BsiHKAI	GWGCWC	1 cut(s)	292
BsiHKCI	CYCGRG	3 cut(s)	177, 671, 1869
BsiSI	CCGG	3 cut(s)	114, 1001, 1663
BslFI	GGGAC	1 cut(s)	1111
BslI	CCNNNNNNNGG	4 cut(s)	1055, 1112, 1285, 1438
BsmAI	GTCTC	4 cut(s)	156, 189, 1064, 1408
BsmFI	GGGAC	1 cut(s)	1111
BsnI	GGCC	3 cut(s)	1431, 1463, 1565
Bso31I	GGTCTC	1 cut(s)	1064
BsoBI	CYCGRG	3 cut(s)	177, 671, 1869
Bsp119I	TTCGAA	4 cut(s)	6, 98, 1167, 1825
Bsp1286I	GDGCHC	1 cut(s)	292
Bsp1407I	TGTACA	1 cut(s)	657
Bsp143I	GATC	7 cut(s)	205, 416, 901, 961, 1318, 1371, 1737
BspACI	CCGC	5 cut(s)	70, 371, 636, 1448, 1666
BspANI	GGCC	3 cut(s)	1431, 1463, 1565
BspCNI	CTCAG	4 cut(s)	321, 380, 1117, 1695
BspHI	TCATGA	2 cut(s)	760, 1848
BspLI	GGNNCC	1 cut(s)	1547
BspMI	ACCTGC	1 cut(s)	1681
BspPI	GGATC	2 cut(s)	213, 1379
BspQI	GCTCTTC	2 cut(s)	233, 1683
BspT104I	TTCGAA	4 cut(s)	6, 98, 1167, 1825
BspT107I	GGYRCC	1 cut(s)	1545
BspTNI	GGTCTC	1 cut(s)	1064
BsrFI	RCCGGY	1 cut(s)	1662
BsrGI	TGTACA	1 cut(s)	657
BsrI	ACTGG	1 cut(s)	983
BssAI	RCCGGY	1 cut(s)	1662
BssECI	CCNNGG	2 cut(s)	489, 490
BssMI	GATC	7 cut(s)	205, 416, 901, 961, 1318, 1371, 1737
BssNI	GRCGYC	1 cut(s)	1638
BssSI	CACGAG	2 cut(s)	1416, 1812
Bst2BI	CACGAG	2 cut(s)	1416, 1812
Bst2UI	CCWGG	3 cut(s)	491, 1286, 1733
Bst4CI	ACNGT	5 cut(s)	29, 62, 358, 1402, 1628
Bst6I	CTCTTC	6 cut(s)	233, 765, 770, 1503, 1590, 1683
BstACI	GRCGYC	1 cut(s)	1638
BstAPI	GCANNNNNTGC	1 cut(s)	482
BstAUI	TGTACA	1 cut(s)	657
BstBAI	YACGTR	1 cut(s)	109
BstBI	TTCGAA	4 cut(s)	6, 98, 1167, 1825
BstC8I	GCNNGC	3 cut(s)	1380, 1599, 1689
BstDEI	CTNAG	4 cut(s)	329, 367, 1125, 1682
BstENI	CCTNNNNNAGG	1 cut(s)	1283
BstF5I	GGATG	2 cut(s)	697, 1861
BstHHI	GCGC	1 cut(s)	398
BstKTI	GATC	7 cut(s)	208, 419, 904, 964, 1321, 1374, 1740
BstMAI	GTCTC	4 cut(s)	156, 189, 1064, 1408
BstMBI	GATC	7 cut(s)	205, 416, 901, 961, 1318, 1371, 1737
BstMWI	GCNNNNNNNGC	2 cut(s)	482, 1201
BstNI	CCWGG	3 cut(s)	491, 1286, 1733
BstNSI	RCATGY	3 cut(s)	1025, 1382, 1601
BstSCI	CCNGG	3 cut(s)	489, 1284, 1731
BstSFI	CTRYAG	1 cut(s)	510
BstSLI	GKGCMC	1 cut(s)	292
BstV1I	GCAGC	4 cut(s)	243, 583, 625, 1168
BstV2I	GAAGAC	1 cut(s)	749
BstX2I	RGATCY	1 cut(s)	416
BstYI	RGATCY	1 cut(s)	416
Bsu36I	CCTNAGG	1 cut(s)	329
BsuRI	GGCC	3 cut(s)	1431, 1463, 1565
BtgZI	GCGATG	1 cut(s)	297
BtsCI	GGATG	2 cut(s)	697, 1861
BveI	ACCTGC	1 cut(s)	1681
Cac8I	GCNNGC	3 cut(s)	1380, 1599, 1689
CciI	TCATGA	2 cut(s)	760, 1848
CfoI	GCGC	1 cut(s)	398
Cfr10I	RCCGGY	1 cut(s)	1662
Cfr13I	GGNCC	2 cut(s)	1262, 1563
Csp6I	GTAC	3 cut(s)	606, 658, 1546
CviQI	GTAC	3 cut(s)	606, 658, 1546
DdeI	CTNAG	4 cut(s)	329, 367, 1125, 1682
DpnI	GATC	7 cut(s)	207, 418, 903, 963, 1320, 1373, 1739
DpnII	GATC	7 cut(s)	205, 416, 901, 961, 1318, 1371, 1737
DraI	TTTAAA	1 cut(s)	451
Eam1104I	CTCTTC	6 cut(s)	233, 765, 770, 1503, 1590, 1683
EarI	CTCTTC	6 cut(s)	233, 765, 770, 1503, 1590, 1683
EciI	GGCGGA	2 cut(s)	1463, 1681
Eco31I	GGTCTC	1 cut(s)	1064
Eco47I	GGWCC	1 cut(s)	1262
Eco57I	CTGAAG	3 cut(s)	867, 999, 1736
Eco81I	CCTNAGG	1 cut(s)	329
Eco88I	CYCGRG	3 cut(s)	177, 671, 1869
EcoNI	CCTNNNNNAGG	1 cut(s)	1283
EcoO109I	RGGNCCY	1 cut(s)	1563
EcoRII	CCWGG	3 cut(s)	489, 1284, 1731
FaqI	GGGAC	1 cut(s)	1111
FauI	CCCGC	1 cut(s)	77
FauNDI	CATATG	1 cut(s)	1357
FbaI	TGATCA	1 cut(s)	1737
Fnu4HI	GCNGC	6 cut(s)	257, 372, 597, 636, 639, 1182
FokI	GGATG	2 cut(s)	704, 1848
Fsp4HI	GCNGC	6 cut(s)	257, 372, 597, 636, 639, 1182
FspBI	CTAG	2 cut(s)	552, 747
GlaI	GCGC	1 cut(s)	397
GluI	GCNGC	6 cut(s)	257, 372, 597, 636, 639, 1182
GsuI	CTGGAG	1 cut(s)	1521
HaeIII	GGCC	3 cut(s)	1431, 1463, 1565
HapII	CCGG	3 cut(s)	114, 1001, 1663
HhaI	GCGC	1 cut(s)	398
Hin1I	GRCGYC	1 cut(s)	1638
Hin6I	GCGC	1 cut(s)	396
HinP1I	GCGC	1 cut(s)	396
HindIII	AAGCTT	4 cut(s)	93, 299, 1408, 1466
HinfI	GANTC	9 cut(s)	33, 182, 379, 461, 829, 994, 1436, 1495, 1709
HpaII	CCGG	3 cut(s)	114, 1001, 1663
HphI	GGTGA	2 cut(s)	1667, 1747
Hpy166II	GTNNAC	3 cut(s)	56, 290, 606
Hpy188I	TCNGA	6 cut(s)	189, 205, 428, 1018, 1214, 1742
Hpy8I	GTNNAC	3 cut(s)	56, 290, 606
Hpy99I	CGWCG	1 cut(s)	1643
HpyAV	CCTTC	3 cut(s)	808, 1048, 1646
HpyCH4III	ACNGT	5 cut(s)	29, 62, 358, 1402, 1628
HpyCH4IV	ACGT	4 cut(s)	78, 108, 1390, 1638
HpyF10VI	GCNNNNNNNGC	2 cut(s)	482, 1201
HpyF3I	CTNAG	4 cut(s)	329, 367, 1125, 1682
HpySE526I	ACGT	4 cut(s)	78, 108, 1390, 1638
Hsp92I	GRCGYC	1 cut(s)	1638
HspAI	GCGC	1 cut(s)	396
KpnI	GGTACC	1 cut(s)	1549
Ksp22I	TGATCA	1 cut(s)	1737
Kzo9I	GATC	7 cut(s)	205, 416, 901, 961, 1318, 1371, 1737
LguI	GCTCTTC	2 cut(s)	233, 1683
LmnI	GCTCC	2 cut(s)	604, 931
Lsp1109I	GCAGC	4 cut(s)	243, 583, 625, 1168
LweI	GCATC	1 cut(s)	691
MaeI	CTAG	2 cut(s)	552, 747
MaeII	ACGT	4 cut(s)	78, 108, 1390, 1638
MaeIII	GTNAC	2 cut(s)	109, 352
MalI	GATC	7 cut(s)	207, 418, 903, 963, 1320, 1373, 1739
MboI	GATC	7 cut(s)	205, 416, 901, 961, 1318, 1371, 1737
MfeI	CAATTG	1 cut(s)	1314
MflI	RGATCY	1 cut(s)	416
MhlI	GDGCHC	1 cut(s)	292
MlyI	GAGTC	3 cut(s)	191, 373, 1445
MmeI	TCCRAC	3 cut(s)	1237, 1507, 1523
MroXI	GAANNNNTTC	1 cut(s)	1821
MseI	TTAA	6 cut(s)	90, 164, 245, 450, 1233, 1802
MslI	CAYNNNNRTG	2 cut(s)	853, 1142
MspA1I	CMGCKG	2 cut(s)	371, 638
MspI	CCGG	3 cut(s)	114, 1001, 1663
MspR9I	CCNGG	3 cut(s)	491, 1286, 1733
MunI	CAATTG	1 cut(s)	1314
MvaI	CCWGG	3 cut(s)	491, 1286, 1733
MwoI	GCNNNNNNNGC	2 cut(s)	482, 1201
NdeI	CATATG	1 cut(s)	1357
NdeII	GATC	7 cut(s)	205, 416, 901, 961, 1318, 1371, 1737
NlaIV	GGNNCC	1 cut(s)	1547
NmuCI	GTSAC	2 cut(s)	109, 352
NspI	RCATGY	3 cut(s)	1025, 1382, 1601
NspV	TTCGAA	4 cut(s)	6, 98, 1167, 1825
PaeI	GCATGC	2 cut(s)	1382, 1601
PaeR7I	CTCGAG	1 cut(s)	1869
PagI	TCATGA	2 cut(s)	760, 1848
PasI	CCCWGGG	1 cut(s)	490
PciSI	GCTCTTC	2 cut(s)	233, 1683
PcsI	WCGNNNNNNNCGW	2 cut(s)	209, 1183
PdmI	GAANNNNTTC	1 cut(s)	1821
PfeI	GAWTC	6 cut(s)	33, 461, 829, 994, 1495, 1709
PkrI	GCNGC	6 cut(s)	258, 373, 598, 637, 640, 1183
PleI	GAGTC	3 cut(s)	190, 373, 1444
PpsI	GAGTC	3 cut(s)	190, 373, 1444
Ppu21I	YACGTR	1 cut(s)	109
PshBI	ATTAAT	1 cut(s)	1233
Psp6I	CCWGG	3 cut(s)	489, 1284, 1731
PspGI	CCWGG	3 cut(s)	489, 1284, 1731
PspN4I	GGNNCC	1 cut(s)	1547
PspPI	GGNCC	2 cut(s)	1262, 1563
PspXI	VCTCGAGB	1 cut(s)	1869
PsuI	RGATCY	1 cut(s)	416
RsaI	GTAC	3 cut(s)	607, 659, 1547
RsaNI	GTAC	3 cut(s)	606, 658, 1546
RseI	CAYNNNNRTG	2 cut(s)	853, 1142
SapI	GCTCTTC	2 cut(s)	233, 1683
SaqAI	TTAA	6 cut(s)	90, 164, 245, 450, 1233, 1802
SatI	GCNGC	6 cut(s)	257, 372, 597, 636, 639, 1182
Sau3AI	GATC	7 cut(s)	205, 416, 901, 961, 1318, 1371, 1737
Sau96I	GGNCC	2 cut(s)	1262, 1563
SchI	GAGTC	3 cut(s)	191, 373, 1445
ScrFI	CCNGG	3 cut(s)	491, 1286, 1733
SduI	GDGCHC	1 cut(s)	292
SfaNI	GCATC	1 cut(s)	691
SfcI	CTRYAG	1 cut(s)	510
Sfr274I	CTCGAG	1 cut(s)	1869
SfuI	TTCGAA	4 cut(s)	6, 98, 1167, 1825
SinI	GGWCC	1 cut(s)	1262
SlaI	CTCGAG	1 cut(s)	1869
SmiMI	CAYNNNNRTG	2 cut(s)	853, 1142
SmlI	CTYRAG	4 cut(s)	1405, 1433, 1469, 1869
SmoI	CTYRAG	4 cut(s)	1405, 1433, 1469, 1869
SphI	GCATGC	2 cut(s)	1382, 1601
SsiI	CCGC	5 cut(s)	70, 371, 636, 1448, 1666
SspI	AATATT	2 cut(s)	1241, 1807
SspMI	CTAG	2 cut(s)	552, 747
StyD4I	CCNGG	3 cut(s)	489, 1284, 1731
TaaI	ACNGT	5 cut(s)	29, 62, 358, 1402, 1628
TaiI	ACGT	4 cut(s)	81, 111, 1393, 1641
TatI	WGTACW	2 cut(s)	605, 657
TauI	GCSGC	2 cut(s)	374, 638
TfiI	GAWTC	6 cut(s)	33, 461, 829, 994, 1495, 1709
Tru1I	TTAA	6 cut(s)	90, 164, 245, 450, 1233, 1802
Tru9I	TTAA	6 cut(s)	90, 164, 245, 450, 1233, 1802
TseFI	GTSAC	2 cut(s)	109, 352
TseI	GCWGC	4 cut(s)	256, 596, 638, 1181
Tsp45I	GTSAC	2 cut(s)	109, 352
TspGWI	ACGGA	2 cut(s)	372, 591
VneI	GTGCAC	1 cut(s)	288
VpaK11BI	GGWCC	1 cut(s)	1262
VspI	ATTAAT	1 cut(s)	1233
XagI	CCTNNNNNAGG	1 cut(s)	1283
XapI	RAATTY	5 cut(s)	122, 135, 1268, 1452, 1530
XceI	RCATGY	3 cut(s)	1025, 1382, 1601
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1117
XhoI	CTCGAG	1 cut(s)	1869
XmnI	GAANNNNTTC	1 cut(s)	1821
XspI	CTAG	2 cut(s)	552, 747
ZraI	GACGTC	1 cut(s)	1639

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.