pycom07g25910

RNA polymerase sigma factor sigE, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008760
Physical Location & Seq
Forward (+)
26076132 .. 26078432
2301 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25910.3

Sequence Viewer

Length: 1560 bp
ATGGGAGTTGTGACAGTTTCAACCTCAGCGGCAAGGACTCCGTTAGGATTGAGCACAAAATCGACAAGTCAGAGATCTCCATTCAGAAAGCCGTGGATAGTTGCGTTTAAAACAGATGAATCCAATAAGACGGCATTAGTTGCACCCCAGGGAAAAATACCTTTGCCTATAGAAACACGAAAGAAGCTCCAAAAGAGAATAGGGAAAGCCAGAAAACTCGCGAAAAGTGTGAAATCTACTGCTACTGATGTAGCTGCTCCGTGTACTACTTTGGAAGTGGATTACAATGAAGCCGCTGCCAAGCTGGAAAACTTATACAAGCGTAGCCCTGAGATTGAAGCTGATGTTGATGTGGAAGATGCAGATGGCTTGTTGAGGAGGGGTCGCAAAAGGAGGAAGAAGACTAGCGAAAGCGATCATGAGAAAGAAGAGAAGAGAACAAGTGATATTGTGGTTAGAAGCAAGACAAAGAAGGCGAAAAGATTGAATCTTGATAAAAGGGTTGCATTGAAATGGAACAAGGACGAGAAAGTGGTTTCTTCGGTTCAAATGAGGAAAGATCAAAAGAACGAAATGGAGAAGATTGAGGAGCTGGTTAGGGAGTATTCGGCTTCAACTGATCTGGTCAGTTTGGACTGGAAAAAGATGAAGATTCCTCCGGTTCTCACTTCTTCAGAGCATGCTTGGTTGTTCAAGCTAATGCAACCTATGAAGGGTCTCCATGAAGTGAGAGAGCAATTACAAAAAGATCTGGGGACAGAACCAACCAACGGCGAATTGGCTGAGGCGACAAACATGAGTGTGGTTCAAGTGAAAAAACATTTCGAAGTTGGTCGAGCTGCACGAAACAAGCTCATCAAGCACAATCTCCGACTTGTGTTATTCGTCATTAATAAATATTTTCAAGACTTTGCAAATGGTCCAAAATTTCAAGACCTGTGCCAGGCAGGAGTAAGGGGGCTTATTACGGCAATTGATCGCTTTGAACCAAAAAGAAGTTTTCGGCTTTCCACATATGGTCTGTACTGGATCAGGCATGCCATCATACGTTCCATGACAGTCTCAAGCTTTACTCGTGTTTCATTTGGCCTTGAGTCGGTTAGGGCGGAAATTCAAAAGGCCAAGCTTGAGATGATGTTTTCGCTCAAAAGGATGCCAACTGACGAAGAGATAATCGAAAAAGTTGGAATTTCGCCAGAGAGGTACCATGAAGTAATGAGGGCCTCAAAACCCGTTTCCTCTCTTCATTCAAGGCATGCAACCACACAAGAGGAGTATATCAACGGTATTACTGACGTCGATGGTGTTGAAGGTGATAACCGGCGGAAACCTGCTCTTCTCAGGCTTGCTATTGATGATGTGCTTGATTCCCTGAAGCCGAAAGAAAGCTTGGTGATCAGACAGAGATACGGGCTTGATGGTAAGGGGGATAGAACATTAGGAGAGATTGCTGGAAACTTAAATATTTCACGAGAAATGGTTCGAAAGCATGAAGTCAAGGCCCTCATGAAGCTCAAACATCCAGCTCGAGTAGATTATCTTCGCAGATACATAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000988 GO:0000990 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006091 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009553 GO:0009628 GO:0009637 GO:0009639 GO:0009651 GO:0009657 GO:0009658 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010114 GO:0010207 GO:0010218 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015979 GO:0016043 GO:0016070 GO:0016987 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019684 GO:0022607 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0046483 GO:0048229 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0071214 GO:0071470 GO:0071472 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0104004 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2000142 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

520

Amino Acids

59.23

Weight (kDa)

9.88

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1299
Acc36I ACCTGC 1 cut(s) 1339
Acc65I GGTACC 1 cut(s) 1203
AccB1I GGYRCC 1 cut(s) 1203
AccII CGCG 1 cut(s) 221
AciI CCGC 4 cut(s) 29, 294, 1106, 1324
AclWI GGATC 1 cut(s) 1037
AcsI RAATTY 3 cut(s) 926, 1110, 1188
AcuI CTGAAG 2 cut(s) 657, 1394
AcyI GRCGYC 1 cut(s) 1296
AfaI GTAC 3 cut(s) 265, 1025, 1205
AfiI CCNNNNNNNGG 4 cut(s) 713, 770, 943, 1096
AjnI CCWGG 2 cut(s) 147, 942
AjuI GAANNNNNNNTTGG 2 cut(s) 1373, 1405
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 2 cut(s) 722, 1066
AlwI GGATC 1 cut(s) 1037
Ama87I CYCGRG 1 cut(s) 1527
AoxI GGCC 4 cut(s) 1087, 1119, 1221, 1500
ApeKI GCWGC 3 cut(s) 254, 296, 839
ApoI RAATTY 3 cut(s) 926, 1110, 1188
AseI ATTAAT 1 cut(s) 891
Asp700I GAANNNNTTC 1 cut(s) 1479
Asp718I GGTACC 1 cut(s) 1203
AspS9I GGNCC 3 cut(s) 920, 1221, 1501
AsuHPI GGTGA 2 cut(s) 1325, 1405
AsuII TTCGAA 2 cut(s) 825, 1483
AvaI CYCGRG 1 cut(s) 1527
AvaII GGWCC 1 cut(s) 920
BanI GGYRCC 1 cut(s) 1203
BarI GAAGNNNNNNTAC 2 cut(s) 1524, 1556
BauI CACGAG 2 cut(s) 1074, 1470
BbsI GAAGAC 1 cut(s) 407
Bbv12I GWGCWC 1 cut(s) 56
BbvCI CCTCAGC 2 cut(s) 25, 783
BbvI GCAGC 3 cut(s) 241, 283, 826
BccI CCATC 4 cut(s) 359, 1049, 1295, 1412
BceAI ACGGC 4 cut(s) 76, 147, 787, 984
BciT130I CCWGG 2 cut(s) 149, 944
BclI TGATCA 1 cut(s) 1395
BcoDI GTCTC 2 cut(s) 722, 1066
BfaI CTAG 1 cut(s) 405
BfmI CTRYAG 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 1339
BglII AGATCT 2 cut(s) 74, 748
BisI GCNGC 5 cut(s) 30, 255, 294, 297, 840
BlsI GCNGC 5 cut(s) 31, 256, 295, 298, 841
Bme1390I CCNGG 2 cut(s) 149, 944
Bme18I GGWCC 1 cut(s) 920
BmeT110I CYCGRG 1 cut(s) 1527
BmgT120I GGNCC 3 cut(s) 920, 1221, 1501
BmiI GGNNCC 1 cut(s) 1205
BmrFI CCNGG 2 cut(s) 149, 944
BmsI GCATC 2 cut(s) 349, 1143
BpiI GAAGAC 1 cut(s) 407
Bpu10I CCTNAGC 2 cut(s) 25, 783
Bpu14I TTCGAA 2 cut(s) 825, 1483
BpuEI CTTGAG 3 cut(s) 1048, 1112, 1148
BsaHI GRCGYC 1 cut(s) 1296
BsaI GGTCTC 1 cut(s) 722
BsaJI CCNNGG 3 cut(s) 92, 147, 148
BsaWI WCCGGW 1 cut(s) 658
Bsc4I CCNNNNNNNGG 4 cut(s) 713, 770, 943, 1096
Bse118I RCCGGY 1 cut(s) 1320
Bse1I ACTGG 2 cut(s) 641, 1031
BseBI CCWGG 2 cut(s) 149, 944
BseDI CCNNGG 3 cut(s) 92, 147, 148
BseGI GGATG 2 cut(s) 1158, 1519
BseLI CCNNNNNNNGG 4 cut(s) 713, 770, 943, 1096
BseMII CTCAG 4 cut(s) 39, 321, 774, 1354
BseNI ACTGG 2 cut(s) 641, 1031
BseRI GAGGAG 3 cut(s) 391, 602, 1286
BseXI GCAGC 3 cut(s) 241, 283, 826
BsgI GTGCAG 1 cut(s) 825
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 4 cut(s) 1089, 1121, 1223, 1502
BshNI GGYRCC 1 cut(s) 1203
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 1527
BsiSI CCGG 2 cut(s) 659, 1321
BslFI GGGAC 1 cut(s) 769
BslI CCNNNNNNNGG 4 cut(s) 713, 770, 943, 1096
BsmAI GTCTC 2 cut(s) 722, 1066
BsmFI GGGAC 1 cut(s) 769
BsnI GGCC 4 cut(s) 1089, 1121, 1223, 1502
Bso31I GGTCTC 1 cut(s) 722
BsoBI CYCGRG 1 cut(s) 1527
Bsp119I TTCGAA 2 cut(s) 825, 1483
Bsp1286I GDGCHC 1 cut(s) 56
Bsp143I GATC 8 cut(s) 74, 415, 559, 619, 748, 976, 1029, 1395
Bsp68I TCGCGA 1 cut(s) 221
BspACI CCGC 4 cut(s) 29, 294, 1106, 1324
BspANI GGCC 4 cut(s) 1089, 1121, 1223, 1502
BspCNI CTCAG 4 cut(s) 38, 322, 775, 1353
BspFNI CGCG 1 cut(s) 221
BspHI TCATGA 2 cut(s) 418, 1506
BspLI GGNNCC 1 cut(s) 1205
BspMI ACCTGC 1 cut(s) 1339
BspPI GGATC 1 cut(s) 1037
BspQI GCTCTTC 1 cut(s) 1341
BspT104I TTCGAA 2 cut(s) 825, 1483
BspT107I GGYRCC 1 cut(s) 1203
BspTNI GGTCTC 1 cut(s) 722
BsrFI RCCGGY 1 cut(s) 1320
BsrI ACTGG 2 cut(s) 641, 1031
BssAI RCCGGY 1 cut(s) 1320
BssECI CCNNGG 3 cut(s) 92, 147, 148
BssMI GATC 8 cut(s) 74, 415, 559, 619, 748, 976, 1029, 1395
BssNI GRCGYC 1 cut(s) 1296
BssSI CACGAG 2 cut(s) 1074, 1470
Bst2BI CACGAG 2 cut(s) 1074, 1470
Bst2UI CCWGG 2 cut(s) 149, 944
Bst4CI ACNGT 3 cut(s) 16, 1060, 1286
Bst6I CTCTTC 5 cut(s) 423, 428, 1161, 1248, 1341
BstACI GRCGYC 1 cut(s) 1296
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBI TTCGAA 2 cut(s) 825, 1483
BstC8I GCNNGC 4 cut(s) 681, 1038, 1257, 1347
BstDEI CTNAG 4 cut(s) 25, 330, 783, 1340
BstDSI CCRYGG 1 cut(s) 92
BstENI CCTNNNNNAGG 1 cut(s) 941
BstF5I GGATG 2 cut(s) 1158, 1519
BstFNI CGCG 1 cut(s) 221
BstKTI GATC 8 cut(s) 77, 418, 562, 622, 751, 979, 1032, 1398
BstMAI GTCTC 2 cut(s) 722, 1066
BstMBI GATC 8 cut(s) 74, 415, 559, 619, 748, 976, 1029, 1395
BstMWI GCNNNNNNNGC 2 cut(s) 140, 859
BstNI CCWGG 2 cut(s) 149, 944
BstNSI RCATGY 3 cut(s) 683, 1040, 1259
BstSCI CCNGG 2 cut(s) 147, 942
BstSFI CTRYAG 1 cut(s) 168
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 3 cut(s) 241, 283, 826
BstV2I GAAGAC 1 cut(s) 407
BstX2I RGATCY 2 cut(s) 74, 748
BstYI RGATCY 2 cut(s) 74, 748
BsuRI GGCC 4 cut(s) 1089, 1121, 1223, 1502
BtgI CCRYGG 1 cut(s) 92
BtsCI GGATG 2 cut(s) 1158, 1519
BtuMI TCGCGA 1 cut(s) 221
BveI ACCTGC 1 cut(s) 1339
Cac8I GCNNGC 4 cut(s) 681, 1038, 1257, 1347
CciI TCATGA 2 cut(s) 418, 1506
Cfr10I RCCGGY 1 cut(s) 1320
Cfr13I GGNCC 3 cut(s) 920, 1221, 1501
Csp6I GTAC 3 cut(s) 264, 1024, 1204
CviQI GTAC 3 cut(s) 264, 1024, 1204
DdeI CTNAG 4 cut(s) 25, 330, 783, 1340
DpnI GATC 8 cut(s) 76, 417, 561, 621, 750, 978, 1031, 1397
DpnII GATC 8 cut(s) 74, 415, 559, 619, 748, 976, 1029, 1395
DraI TTTAAA 1 cut(s) 109
Eam1104I CTCTTC 5 cut(s) 423, 428, 1161, 1248, 1341
EarI CTCTTC 5 cut(s) 423, 428, 1161, 1248, 1341
EciI GGCGGA 2 cut(s) 1121, 1339
Eco31I GGTCTC 1 cut(s) 722
Eco47I GGWCC 1 cut(s) 920
Eco57I CTGAAG 2 cut(s) 657, 1394
Eco88I CYCGRG 1 cut(s) 1527
EcoNI CCTNNNNNAGG 1 cut(s) 941
EcoO109I RGGNCCY 2 cut(s) 1221, 1501
EcoRII CCWGG 2 cut(s) 147, 942
FaqI GGGAC 1 cut(s) 769
FauNDI CATATG 1 cut(s) 1015
FbaI TGATCA 1 cut(s) 1395
Fnu4HI GCNGC 5 cut(s) 30, 255, 294, 297, 840
FokI GGATG 2 cut(s) 1165, 1506
Fsp4HI GCNGC 5 cut(s) 30, 255, 294, 297, 840
FspBI CTAG 1 cut(s) 405
GluI GCNGC 5 cut(s) 30, 255, 294, 297, 840
HaeIII GGCC 4 cut(s) 1089, 1121, 1223, 1502
HapII CCGG 2 cut(s) 659, 1321
Hin1I GRCGYC 1 cut(s) 1296
HindIII AAGCTT 3 cut(s) 1066, 1124, 1387
HinfI GANTC 6 cut(s) 37, 119, 487, 652, 1094, 1367
HpaII CCGG 2 cut(s) 659, 1321
HphI GGTGA 2 cut(s) 1325, 1405
Hpy166II GTNNAC 1 cut(s) 264
Hpy188I TCNGA 5 cut(s) 72, 86, 676, 872, 1400
Hpy188III TCNNGA 7 cut(s) 220, 419, 491, 905, 932, 1470, 1507
Hpy8I GTNNAC 1 cut(s) 264
Hpy99I CGWCG 1 cut(s) 1301
HpyAV CCTTC 3 cut(s) 466, 706, 1304
HpyCH4III ACNGT 3 cut(s) 16, 1060, 1286
HpyCH4IV ACGT 2 cut(s) 1048, 1296
HpyCH4V TGCA 7 cut(s) 143, 362, 506, 703, 842, 914, 1259
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 859
HpyF3I CTNAG 4 cut(s) 25, 330, 783, 1340
HpySE526I ACGT 2 cut(s) 1048, 1296
Hsp92I GRCGYC 1 cut(s) 1296
KpnI GGTACC 1 cut(s) 1207
Ksp22I TGATCA 1 cut(s) 1395
Kzo9I GATC 8 cut(s) 74, 415, 559, 619, 748, 976, 1029, 1395
LguI GCTCTTC 1 cut(s) 1341
LmnI GCTCC 3 cut(s) 192, 262, 589
Lsp1109I GCAGC 3 cut(s) 241, 283, 826
LweI GCATC 2 cut(s) 349, 1143
MaeI CTAG 1 cut(s) 405
MaeII ACGT 2 cut(s) 1048, 1296
MaeIII GTNAC 1 cut(s) 10
MalI GATC 8 cut(s) 76, 417, 561, 621, 750, 978, 1031, 1397
MboI GATC 8 cut(s) 74, 415, 559, 619, 748, 976, 1029, 1395
MfeI CAATTG 1 cut(s) 972
MflI RGATCY 2 cut(s) 74, 748
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 6 cut(s) 737, 776, 926, 972, 1110, 1188
MlyI GAGTC 2 cut(s) 31, 1103
MmeI TCCRAC 2 cut(s) 895, 1165
MroXI GAANNNNTTC 1 cut(s) 1479
MseI TTAA 3 cut(s) 108, 891, 1460
MslI CAYNNNNRTG 2 cut(s) 511, 800
MspA1I CMGCKG 2 cut(s) 29, 296
MspI CCGG 2 cut(s) 659, 1321
MspR9I CCNGG 2 cut(s) 149, 944
MunI CAATTG 1 cut(s) 972
MvaI CCWGG 2 cut(s) 149, 944
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 140, 859
NdeI CATATG 1 cut(s) 1015
NdeII GATC 8 cut(s) 74, 415, 559, 619, 748, 976, 1029, 1395
NlaIV GGNNCC 1 cut(s) 1205
NmuCI GTSAC 1 cut(s) 10
NruI TCGCGA 1 cut(s) 221
NspI RCATGY 3 cut(s) 683, 1040, 1259
NspV TTCGAA 2 cut(s) 825, 1483
PaeI GCATGC 3 cut(s) 683, 1040, 1259
PaeR7I CTCGAG 1 cut(s) 1527
PagI TCATGA 2 cut(s) 418, 1506
PasI CCCWGGG 1 cut(s) 148
PciSI GCTCTTC 1 cut(s) 1341
PcsI WCGNNNNNNNCGW 1 cut(s) 841
PdmI GAANNNNTTC 1 cut(s) 1479
PfeI GAWTC 4 cut(s) 119, 487, 652, 1367
PkrI GCNGC 5 cut(s) 31, 256, 295, 298, 841
PleI GAGTC 2 cut(s) 31, 1102
PpsI GAGTC 2 cut(s) 31, 1102
PshBI ATTAAT 1 cut(s) 891
Psp6I CCWGG 2 cut(s) 147, 942
PspGI CCWGG 2 cut(s) 147, 942
PspN4I GGNNCC 1 cut(s) 1205
PspPI GGNCC 3 cut(s) 920, 1221, 1501
PspXI VCTCGAGB 1 cut(s) 1527
PsuI RGATCY 2 cut(s) 74, 748
RruI TCGCGA 1 cut(s) 221
RsaI GTAC 3 cut(s) 265, 1025, 1205
RsaNI GTAC 3 cut(s) 264, 1024, 1204
RseI CAYNNNNRTG 2 cut(s) 511, 800
SapI GCTCTTC 1 cut(s) 1341
SaqAI TTAA 3 cut(s) 108, 891, 1460
SatI GCNGC 5 cut(s) 30, 255, 294, 297, 840
Sau3AI GATC 8 cut(s) 74, 415, 559, 619, 748, 976, 1029, 1395
Sau96I GGNCC 3 cut(s) 920, 1221, 1501
SchI GAGTC 2 cut(s) 31, 1103
ScrFI CCNGG 2 cut(s) 149, 944
SduI GDGCHC 1 cut(s) 56
SfaNI GCATC 2 cut(s) 349, 1143
SfcI CTRYAG 1 cut(s) 168
Sfr274I CTCGAG 1 cut(s) 1527
SfuI TTCGAA 2 cut(s) 825, 1483
SinI GGWCC 1 cut(s) 920
SlaI CTCGAG 1 cut(s) 1527
SmiMI CAYNNNNRTG 2 cut(s) 511, 800
SmlI CTYRAG 4 cut(s) 1063, 1091, 1127, 1527
SmoI CTYRAG 4 cut(s) 1063, 1091, 1127, 1527
SphI GCATGC 3 cut(s) 683, 1040, 1259
Sse9I AATT 6 cut(s) 737, 776, 926, 972, 1110, 1188
SsiI CCGC 4 cut(s) 29, 294, 1106, 1324
SspI AATATT 2 cut(s) 899, 1465
SspMI CTAG 1 cut(s) 405
StyD4I CCNGG 2 cut(s) 147, 942
TaaI ACNGT 3 cut(s) 16, 1060, 1286
TaiI ACGT 2 cut(s) 1051, 1299
TaqI TCGA 7 cut(s) 62, 825, 835, 1176, 1299, 1483, 1528
TasI AATT 6 cut(s) 737, 776, 926, 972, 1110, 1188
TatI WGTACW 2 cut(s) 263, 1023
TauI GCSGC 2 cut(s) 32, 296
TfiI GAWTC 4 cut(s) 119, 487, 652, 1367
Tru1I TTAA 3 cut(s) 108, 891, 1460
Tru9I TTAA 3 cut(s) 108, 891, 1460
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 3 cut(s) 254, 296, 839
Tsp45I GTSAC 1 cut(s) 10
TspGWI ACGGA 2 cut(s) 30, 249
VpaK11BI GGWCC 1 cut(s) 920
VspI ATTAAT 1 cut(s) 891
XagI CCTNNNNNAGG 1 cut(s) 941
XapI RAATTY 3 cut(s) 926, 1110, 1188
XceI RCATGY 3 cut(s) 683, 1040, 1259
XcmI CCANNNNNNNNNTGG 1 cut(s) 775
XhoI CTCGAG 1 cut(s) 1527
XmnI GAANNNNTTC 1 cut(s) 1479
XspI CTAG 1 cut(s) 405
ZraI GACGTC 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.