Rw1G038460

RNA polymerase sigma factor sigE, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008760
Physical Location & Seq
Forward (+)
65993953 .. 65996210
2258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G038460.1

Sequence Viewer

Length: 1494 bp
ATGGGAGTTGTGACAGTTTCTAGCTCAGTTTCTAGGACTCCGTTAGGATTGAGCCCAAAATTGGCATCAAGTCAGAGATCAACGTTGAGAAAGCCATTGATTGTTGCCTTTAGAACAGATAAATCCAATAAGAACGCGTTGGTTGCGCCTCAGGAGAAAGTACCTTTGCCAATAGAAACACGAAAGAAGGTCCAAAAACTAGGGAAGGCTCGAAAGCTCCCCGAAAGTGTAAAAGCTGCATTGATAGAGGAGGCTCCTACTTGTAATTTGGAACTGGATTACAATGAAGCTGCTGCCAAGCTGGAAAATCTGTACAAGCGCAGCCCCGAGACTGAAGCTTCGGATTTGGAAAATGTAGATGGATTGATGATCAAGAGAAGTCGCAAAAGGAGGAAGAAGATCAGTGAAGTTGATGACAAAGTAGAGAAGAGAAAAAGCGATGACATTGTGGTTGGGAGCAGGACAAAGAAGGCTAAAAGATTGAGTCTTGATAAAAGGGTTGCGTTGAAATGGAATAAGGACGAGAAAGTGGCTGCTCCAGTAGTTCGAAAGAGAAAAGATAGAAAGAGTGAGAATCAGATGATTGAGGAGCTTGTAAGGGAGTATTCGGCTTCAACTGATCTCGTCAGTTTAGACTGGAAAAAGATGAGGATTCCTCCAGTTCTTACTTCTTCTGAGCATACTTGGTTGTTCAAGCTAATGCAACCTATGAAGGCTCTTCTTCAAGTGAAAGAAACTCTGCAGAAAGATTTGGGGAGAGAACCAAATAAGGGTGAACTAGCTGAGGCGACAAGTATGAGTGTGGTTCAAGTGAAAAAACACTTGGAGGTTGGTCGAGCTGCAAGAAACAAACTCATCAAGCATAATCTCCGGCTTGTGTTATTTGTCATTAACAAATACTTTCAAGACTTTGCGAATGGTCCAAAATTTCAAGACCTTTGTCAGGCAGGAGTGAAGGGACTTATACGATTCCGGCTTTCCACATATGGTCTTTTCTGGATCAGGCATGCCATCATACGTTCCATGACACTATCAAGCTTTACTCGTGTCTCCTTTGGCCTTGAGTCTGTTAGGGCGGAAATCCAGAAAGCCAAGCTTGAAATGTTGGTTCAGCTTAAAAGAATGCCAACAGAGGACGAGATAATTGAAAAGGTTGGAATTTCCCCTGAGAGGTATCATGAAGTAATGAGGGCCTCAAAACCTGAGGAATTTATAAACGCTATTACTGATGTTGACGGCGTTGAAGGTGATAACCGGCGGAAACCTGCTCTTCTCAGGCTTGCTATCGATGATGTGCTTGATTCCCTGAAGCCAAAAGAAAGCTTGGTGATCAGGCAGAGATATGGCCTTGATGGCAAGGGTGACAGAACATTGGGGGAGATAGCTGGAAACTTGAATATTTCAAGAGAAATGGTTCGAAAGCATGAAGTTAAGGCCCTCATGAAGCTCAAGCATCCAGCTCGAGTAGACTATCTTCGCAGATACATAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000988 GO:0000990 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006091 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009553 GO:0009628 GO:0009637 GO:0009639 GO:0009651 GO:0009657 GO:0009658 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010114 GO:0010207 GO:0010218 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015979 GO:0016043 GO:0016070 GO:0016987 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019684 GO:0022607 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0046483 GO:0048229 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0071214 GO:0071470 GO:0071472 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0104004 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2000142 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

497

Amino Acids

56.7

Weight (kDa)

9.99

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sigma70_r3 PF04539 240 - 280 9.8e-06 Sigma-70 region 3
Sigma70_r3 PF04539 359 - 400 2.3e-06 Sigma-70 region 3
Sigma70_r4 PF04545 432 - 485 9.7e-14 Sigma-70, region 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1214
Acc36I ACCTGC 1 cut(s) 1273
AccI GTMKAC 1 cut(s) 1467
AccII CGCG 1 cut(s) 137
AciI CCGC 2 cut(s) 1076, 1258
AclI AACGTT 1 cut(s) 83
AclWI GGATC 1 cut(s) 1007
AcsI RAATTY 3 cut(s) 926, 1158, 1208
AcuI CTGAAG 2 cut(s) 354, 1328
AfaI GTAC 2 cut(s) 162, 314
AfiI CCNNNNNNNGG 4 cut(s) 61, 770, 943, 1170
AflIII ACRYGT 1 cut(s) 135
AjuI GAANNNNNNNTTGG 2 cut(s) 806, 838
Alw26I GTCTC 2 cut(s) 323, 1054
AlwI GGATC 1 cut(s) 1007
Ama87I CYCGRG 2 cut(s) 326, 1461
AoxI GGCC 4 cut(s) 1057, 1191, 1345, 1434
ApeKI GCWGC 6 cut(s) 236, 290, 293, 321, 533, 839
ApoI RAATTY 3 cut(s) 926, 1158, 1208
ArsI GACNNNNNNTTYG 2 cut(s) 322, 354
Asp700I GAANNNNTTC 1 cut(s) 1413
AspLEI GCGC 2 cut(s) 148, 321
AspS9I GGNCC 4 cut(s) 190, 920, 1191, 1435
AsuHPI GGTGA 4 cut(s) 785, 1259, 1339, 1373
AsuII TTCGAA 2 cut(s) 547, 1417
AvaI CYCGRG 2 cut(s) 326, 1461
AvaII GGWCC 2 cut(s) 190, 920
AxyI CCTNAGG 2 cut(s) 150, 1203
BanII GRGCYC 1 cut(s) 56
BarI GAAGNNNNNNTAC 2 cut(s) 1458, 1490
BauI CACGAG 1 cut(s) 1044
BbvCI CCTCAGC 1 cut(s) 783
BbvI GCAGC 6 cut(s) 223, 277, 280, 333, 520, 826
BccI CCATC 3 cut(s) 353, 1019, 1346
BceAI ACGGC 1 cut(s) 1252
BcgI CGANNNNNNTGC 2 cut(s) 1442, 1476
BclI TGATCA 2 cut(s) 369, 1329
BcoDI GTCTC 2 cut(s) 323, 1054
BfaI CTAG 4 cut(s) 21, 33, 200, 779
BfmI CTRYAG 1 cut(s) 740
BfuAI ACCTGC 1 cut(s) 1273
BglI GCCNNNNNGGC 1 cut(s) 1353
BisI GCNGC 6 cut(s) 237, 291, 294, 322, 534, 840
BlsI GCNGC 6 cut(s) 238, 292, 295, 323, 535, 841
Bme18I GGWCC 2 cut(s) 190, 920
BmeT110I CYCGRG 2 cut(s) 326, 1461
BmgT120I GGNCC 4 cut(s) 190, 920, 1191, 1435
BmiI GGNNCC 1 cut(s) 255
BmsI GCATC 2 cut(s) 74, 1462
BoxI GACNNNNGTC 1 cut(s) 939
BplI GAGNNNNNCTC 2 cut(s) 640, 672
BpmI CTGGAG 2 cut(s) 522, 642
Bpu10I CCTNAGC 1 cut(s) 783
Bpu14I TTCGAA 2 cut(s) 547, 1417
BpuEI CTTGAG 2 cut(s) 1082, 1433
Bsa29I ATCGAT 1 cut(s) 1287
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 770, 943, 1170
Bse118I RCCGGY 1 cut(s) 1254
Bse1I ACTGG 4 cut(s) 279, 539, 641, 659
Bse21I CCTNAGG 2 cut(s) 150, 1203
BseCI ATCGAT 1 cut(s) 1287
BseGI GGATG 1 cut(s) 1453
BseLI CCNNNNNNNGG 4 cut(s) 61, 770, 943, 1170
BseMII CTCAG 7 cut(s) 39, 164, 666, 774, 1158, 1194, 1288
BseNI ACTGG 4 cut(s) 279, 539, 641, 659
BseRI GAGGAG 2 cut(s) 263, 602
BseXI GCAGC 6 cut(s) 223, 277, 280, 333, 520, 826
Bsh1236I CGCG 1 cut(s) 137
BshFI GGCC 4 cut(s) 1059, 1193, 1347, 1436
BshVI ATCGAT 1 cut(s) 1287
BsiHKCI CYCGRG 2 cut(s) 326, 1461
BsiSI CCGG 3 cut(s) 871, 973, 1255
BslFI GGGAC 1 cut(s) 972
BslI CCNNNNNNNGG 4 cut(s) 61, 770, 943, 1170
BsmAI GTCTC 2 cut(s) 323, 1054
BsmFI GGGAC 1 cut(s) 972
BsmI GAATGC 1 cut(s) 1128
BsnI GGCC 4 cut(s) 1059, 1193, 1347, 1436
BsoBI CYCGRG 2 cut(s) 326, 1461
Bsp119I TTCGAA 2 cut(s) 547, 1417
Bsp1286I GDGCHC 1 cut(s) 56
Bsp1407I TGTACA 1 cut(s) 312
Bsp143I GATC 6 cut(s) 77, 369, 399, 619, 999, 1329
BspACI CCGC 2 cut(s) 1076, 1258
BspANI GGCC 4 cut(s) 1059, 1193, 1347, 1436
BspCNI CTCAG 7 cut(s) 38, 163, 667, 775, 1159, 1195, 1287
BspDI ATCGAT 1 cut(s) 1287
BspFNI CGCG 1 cut(s) 137
BspHI TCATGA 2 cut(s) 1177, 1440
BspLI GGNNCC 1 cut(s) 255
BspMAI CTGCAG 1 cut(s) 744
BspMI ACCTGC 1 cut(s) 1273
BspPI GGATC 1 cut(s) 1007
BspQI GCTCTTC 2 cut(s) 723, 1275
BspT104I TTCGAA 2 cut(s) 547, 1417
BsrFI RCCGGY 1 cut(s) 1254
BsrGI TGTACA 1 cut(s) 312
BsrI ACTGG 4 cut(s) 279, 539, 641, 659
BssAI RCCGGY 1 cut(s) 1254
BssMI GATC 6 cut(s) 77, 369, 399, 619, 999, 1329
BssSI CACGAG 1 cut(s) 1044
Bst2BI CACGAG 1 cut(s) 1044
Bst4CI ACNGT 1 cut(s) 16
Bst6I CTCTTC 3 cut(s) 422, 723, 1275
BstAUI TGTACA 1 cut(s) 312
BstBI TTCGAA 2 cut(s) 547, 1417
BstC8I GCNNGC 2 cut(s) 1008, 1281
BstDEI CTNAG 7 cut(s) 25, 150, 675, 783, 1167, 1203, 1274
BstENI CCTNNNNNAGG 1 cut(s) 941
BstF5I GGATG 1 cut(s) 1453
BstFNI CGCG 1 cut(s) 137
BstHHI GCGC 2 cut(s) 148, 321
BstKTI GATC 6 cut(s) 80, 372, 402, 622, 1002, 1332
BstMAI GTCTC 2 cut(s) 323, 1054
BstMBI GATC 6 cut(s) 77, 369, 399, 619, 999, 1329
BstMWI GCNNNNNNNGC 2 cut(s) 143, 1353
BstNSI RCATGY 1 cut(s) 1010
BstPAI GACNNNNGTC 1 cut(s) 939
BstSFI CTRYAG 1 cut(s) 740
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 6 cut(s) 223, 277, 280, 333, 520, 826
Bsu15I ATCGAT 1 cut(s) 1287
Bsu36I CCTNAGG 2 cut(s) 150, 1203
BsuRI GGCC 4 cut(s) 1059, 1193, 1347, 1436
BsuTUI ATCGAT 1 cut(s) 1287
BtgZI GCGATG 1 cut(s) 453
BtsCI GGATG 1 cut(s) 1453
BtsIMutI CAGTG 1 cut(s) 409
BveI ACCTGC 1 cut(s) 1273
Cac8I GCNNGC 2 cut(s) 1008, 1281
CciI TCATGA 2 cut(s) 1177, 1440
CfoI GCGC 2 cut(s) 148, 321
Cfr10I RCCGGY 1 cut(s) 1254
Cfr13I GGNCC 4 cut(s) 190, 920, 1191, 1435
ClaI ATCGAT 1 cut(s) 1287
Csp6I GTAC 2 cut(s) 161, 313
CviAII CATG 5 cut(s) 1007, 1024, 1178, 1424, 1441
CviQI GTAC 2 cut(s) 161, 313
DdeI CTNAG 7 cut(s) 25, 150, 675, 783, 1167, 1203, 1274
DpnI GATC 6 cut(s) 79, 371, 401, 621, 1001, 1331
DpnII GATC 6 cut(s) 77, 369, 399, 619, 999, 1329
Eam1104I CTCTTC 3 cut(s) 422, 723, 1275
EarI CTCTTC 3 cut(s) 422, 723, 1275
EciI GGCGGA 2 cut(s) 1091, 1273
Eco24I GRGCYC 1 cut(s) 56
Eco47I GGWCC 2 cut(s) 190, 920
Eco57I CTGAAG 2 cut(s) 354, 1328
Eco81I CCTNAGG 2 cut(s) 150, 1203
Eco88I CYCGRG 2 cut(s) 326, 1461
EcoNI CCTNNNNNAGG 1 cut(s) 941
EcoO109I RGGNCCY 2 cut(s) 1191, 1435
EcoT38I GRGCYC 1 cut(s) 56
FaeI CATG 5 cut(s) 1010, 1027, 1181, 1427, 1444
FalI AAGNNNNNCTT 2 cut(s) 1080, 1112
FaqI GGGAC 1 cut(s) 972
FatI CATG 5 cut(s) 1006, 1023, 1177, 1423, 1440
FauNDI CATATG 1 cut(s) 985
FbaI TGATCA 2 cut(s) 369, 1329
FblI GTMKAC 1 cut(s) 1467
Fnu4HI GCNGC 6 cut(s) 237, 291, 294, 322, 534, 840
FokI GGATG 1 cut(s) 1440
FriOI GRGCYC 1 cut(s) 56
Fsp4HI GCNGC 6 cut(s) 237, 291, 294, 322, 534, 840
FspBI CTAG 4 cut(s) 21, 33, 200, 779
GlaI GCGC 2 cut(s) 147, 320
GluI GCNGC 6 cut(s) 237, 291, 294, 322, 534, 840
GsuI CTGGAG 2 cut(s) 522, 642
HaeIII GGCC 4 cut(s) 1059, 1193, 1347, 1436
HapII CCGG 3 cut(s) 871, 973, 1255
HhaI GCGC 2 cut(s) 148, 321
Hin1II CATG 5 cut(s) 1010, 1027, 1181, 1427, 1444
Hin6I GCGC 2 cut(s) 146, 319
HinP1I GCGC 2 cut(s) 146, 319
HincII GTYRAC 1 cut(s) 1234
HindII GTYRAC 1 cut(s) 1234
HindIII AAGCTT 4 cut(s) 336, 1036, 1094, 1321
HinfI GANTC 7 cut(s) 37, 484, 574, 652, 969, 1064, 1301
HpaII CCGG 3 cut(s) 871, 973, 1255
HphI GGTGA 4 cut(s) 785, 1259, 1339, 1373
Hpy166II GTNNAC 3 cut(s) 776, 1234, 1468
Hpy188I TCNGA 4 cut(s) 75, 343, 579, 676
Hpy8I GTNNAC 3 cut(s) 776, 1234, 1468
HpyAV CCTTC 6 cut(s) 181, 199, 463, 706, 949, 1238
HpyCH4III ACNGT 1 cut(s) 16
HpyCH4IV ACGT 2 cut(s) 83, 1018
HpyCH4V TGCA 4 cut(s) 239, 703, 742, 842
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 1353
HpyF3I CTNAG 7 cut(s) 25, 150, 675, 783, 1167, 1203, 1274
HpySE526I ACGT 2 cut(s) 83, 1018
Hsp92II CATG 5 cut(s) 1010, 1027, 1181, 1427, 1444
HspAI GCGC 2 cut(s) 146, 319
Ksp22I TGATCA 2 cut(s) 369, 1329
Kzo9I GATC 6 cut(s) 77, 369, 399, 619, 999, 1329
LguI GCTCTTC 2 cut(s) 723, 1275
LmnI GCTCC 5 cut(s) 222, 259, 456, 541, 589
Lsp1109I GCAGC 6 cut(s) 223, 277, 280, 333, 520, 826
LweI GCATC 2 cut(s) 74, 1462
MaeI CTAG 4 cut(s) 21, 33, 200, 779
MaeII ACGT 2 cut(s) 83, 1018
MaeIII GTNAC 2 cut(s) 10, 1361
MalI GATC 6 cut(s) 79, 371, 401, 621, 1001, 1331
MboI GATC 6 cut(s) 77, 369, 399, 619, 999, 1329
MboII GAAGA 8 cut(s) 406, 409, 439, 663, 710, 713, 1262, 1466
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 6 cut(s) 59, 265, 926, 1143, 1158, 1208
MluI ACGCGT 1 cut(s) 135
MlyI GAGTC 3 cut(s) 31, 493, 1073
MmeI TCCRAC 1 cut(s) 1135
MroXI GAANNNNTTC 1 cut(s) 1413
MseI TTAA 3 cut(s) 891, 1116, 1431
MspI CCGG 3 cut(s) 871, 973, 1255
Mva1269I GAATGC 1 cut(s) 1128
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 2 cut(s) 143, 1353
NdeI CATATG 1 cut(s) 985
NdeII GATC 6 cut(s) 77, 369, 399, 619, 999, 1329
NlaIII CATG 5 cut(s) 1010, 1027, 1181, 1427, 1444
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 2 cut(s) 10, 1361
NspI RCATGY 1 cut(s) 1010
NspV TTCGAA 2 cut(s) 547, 1417
PaeI GCATGC 1 cut(s) 1010
PaeR7I CTCGAG 1 cut(s) 1461
PagI TCATGA 2 cut(s) 1177, 1440
PciSI GCTCTTC 2 cut(s) 723, 1275
PctI GAATGC 1 cut(s) 1128
PdmI GAANNNNTTC 1 cut(s) 1413
PfeI GAWTC 4 cut(s) 574, 652, 969, 1301
PkrI GCNGC 6 cut(s) 238, 292, 295, 323, 535, 841
PleI GAGTC 3 cut(s) 31, 492, 1072
PpsI GAGTC 3 cut(s) 31, 492, 1072
PshAI GACNNNNGTC 1 cut(s) 939
PsiI TTATAA 1 cut(s) 1214
Psp1406I AACGTT 1 cut(s) 83
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 4 cut(s) 190, 920, 1191, 1435
PspXI VCTCGAGB 1 cut(s) 1461
PstI CTGCAG 1 cut(s) 744
RsaI GTAC 2 cut(s) 162, 314
RsaNI GTAC 2 cut(s) 161, 313
SapI GCTCTTC 2 cut(s) 723, 1275
SaqAI TTAA 3 cut(s) 891, 1116, 1431
SatI GCNGC 6 cut(s) 237, 291, 294, 322, 534, 840
Sau3AI GATC 6 cut(s) 77, 369, 399, 619, 999, 1329
Sau96I GGNCC 4 cut(s) 190, 920, 1191, 1435
SchI GAGTC 3 cut(s) 31, 493, 1073
SduI GDGCHC 1 cut(s) 56
SfaNI GCATC 2 cut(s) 74, 1462
SfcI CTRYAG 1 cut(s) 740
Sfr274I CTCGAG 1 cut(s) 1461
SfuI TTCGAA 2 cut(s) 547, 1417
SinI GGWCC 2 cut(s) 190, 920
SlaI CTCGAG 1 cut(s) 1461
SmlI CTYRAG 3 cut(s) 1061, 1448, 1461
SmoI CTYRAG 3 cut(s) 1061, 1448, 1461
SphI GCATGC 1 cut(s) 1010
Sse9I AATT 6 cut(s) 59, 265, 926, 1143, 1158, 1208
SsiI CCGC 2 cut(s) 1076, 1258
SspI AATATT 1 cut(s) 1399
SspMI CTAG 4 cut(s) 21, 33, 200, 779
TaaI ACNGT 1 cut(s) 16
TaiI ACGT 2 cut(s) 86, 1021
TaqI TCGA 6 cut(s) 211, 547, 835, 1287, 1417, 1462
TasI AATT 6 cut(s) 59, 265, 926, 1143, 1158, 1208
TatI WGTACW 1 cut(s) 312
TfiI GAWTC 4 cut(s) 574, 652, 969, 1301
Tru1I TTAA 3 cut(s) 891, 1116, 1431
Tru9I TTAA 3 cut(s) 891, 1116, 1431
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 2 cut(s) 10, 1361
TseI GCWGC 6 cut(s) 236, 290, 293, 321, 533, 839
Tsp45I GTSAC 2 cut(s) 10, 1361
TspDTI ATGAA 5 cut(s) 300, 725, 1194, 1440, 1457
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 1 cut(s) 409
VpaK11BI GGWCC 2 cut(s) 190, 920
XagI CCTNNNNNAGG 1 cut(s) 941
XapI RAATTY 3 cut(s) 926, 1158, 1208
XceI RCATGY 1 cut(s) 1010
XhoI CTCGAG 1 cut(s) 1461
XmiI GTMKAC 1 cut(s) 1467
XmnI GAANNNNTTC 1 cut(s) 1413
XspI CTAG 4 cut(s) 21, 33, 200, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.