MD07G1292400.v1.1

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0018087
Physical Location & Seq
Forward (+)
35363889 .. 35366550
2662 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1292400.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGTTTTCATTTTTGTTGTTATTATTATTATTTTTTCAGGTGAAGCTCGTTCACTGAAAGTAGAAAATATTCTGTATAGGGGGAAATCAGAGTTCCAAGAGATTTTGGTGTTTGAGTCCTCAATATATGGAAAAGTCCTTGTTCTTGATGGCCTTGTCCAGCTGAGTGAGAAAGATGAATGTGCATACCAGGAGATGATAGCACATCTACCTCTTTGTTCAATTCCATCCCCCAAGACAGTTCTGGTTGTTGGCGATGGTGATGGTGGGGTTCTTAGGGAGGTTTCTCGCCATCCTTCTGTTGAGCATATTGATATATGTGAGATAGATAAGATGGTTATTGATGTGTCCAAGAAGTTTTTTCCTCGGTTGGCTGTTGGATTTGAGGACCCTCGCGTGCACCTTCACATCGCTGATGGTCCCGCCCAAGAGCTTGTAGAGAAGCCATTTTTTGAGACAATAGCTAGAGCATTAAGGCCTGGTGGTGTTCTCTGTAACATGGCAGAGAGCATGTGGCTCCATACACATCTTATTCAAGATCTGATCTCTGTTTGCCATCAAACATTCAAGGGGTCTGTCGAATATGCTTGGGCGAGTGTTCCTACGTATCCAAGTGGTGTGATAGGTTTTCTGCTGTGCTCAACAGCGTGGCCTCCCGTTGATTTCAAAAACCCCGTCAATTCTATTGAGAAGTTAGAAGGAGCTCTCAAACATAAGAGAGAACTCCAGTTCTATAACTCCGAGATGCACTCAGCTGCCTTTGCGTTGCCTTCTTTCTTGAGAAGGGAGGTGAGTGCGCTACATGAATCTTCAACTCCGGCAAGACAAATCGGGGATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

281

Amino Acids

31.28

Weight (kDa)

5.77

Isoelectric Point (pI)

50.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synt_N PF17284 14 - 57 3.6e-17 Spermidine synthase tetramerisation domain
Spermine_synth PF01564 60 - 140 9.2e-34 Spermine/spermidine synthase domain
Spermine_synth PF01564 140 - 224 2.9e-18 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018087)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g31860
malus_domestica MD07G1292400.v1.1
prunus_persica Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1
pyrus_communis pycom01g23060
rosa_laevigata RLG00000026245
rosa_rugosa Rorug01G0427500 Rorug01G0427700
rosa_samantha Rh1AG450700 Rh1BG406900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 396
AciI CCGC 1 cut(s) 423
AgsI TTSAA 5 cut(s) 222, 536, 568, 667, 813
AjnI CCWGG 2 cut(s) 189, 478
AluBI AGCT 6 cut(s) 47, 163, 433, 464, 704, 755
AluI AGCT 6 cut(s) 47, 163, 433, 464, 704, 755
Alw21I GWGCWC 3 cut(s) 402, 641, 706
Alw26I GTCTC 1 cut(s) 449
Alw44I GTGCAC 1 cut(s) 398
AoxI GGCC 3 cut(s) 151, 476, 650
ApaLI GTGCAC 1 cut(s) 398
ApeKI GCWGC 1 cut(s) 755
ArsI GACNNNNNNTTYG 2 cut(s) 660, 692
Asp700I GAANNNNTTC 1 cut(s) 69
AspLEI GCGC 1 cut(s) 799
AspS9I GGNCC 2 cut(s) 388, 419
AsuHPI GGTGA 3 cut(s) 53, 272, 802
AvaII GGWCC 2 cut(s) 388, 419
BaeGI GKGCMC 1 cut(s) 402
BanII GRGCYC 1 cut(s) 706
Bbv12I GWGCWC 3 cut(s) 402, 641, 706
BbvI GCAGC 1 cut(s) 742
BccI CCATC 8 cut(s) 143, 235, 251, 257, 300, 328, 410, 564
BciT130I CCWGG 2 cut(s) 191, 480
BciVI GTATCC 1 cut(s) 618
BcoDI GTCTC 1 cut(s) 449
BfaI CTAG 1 cut(s) 465
BfuI GTATCC 1 cut(s) 618
BglII AGATCT 1 cut(s) 538
BisI GCNGC 1 cut(s) 756
BlsI GCNGC 1 cut(s) 757
Bme1390I CCNGG 2 cut(s) 191, 480
Bme18I GGWCC 2 cut(s) 388, 419
BmgT120I GGNCC 2 cut(s) 388, 419
BmiI GGNNCC 3 cut(s) 390, 421, 518
BmrFI CCNGG 2 cut(s) 191, 480
BmsI GCATC 1 cut(s) 735
BplI GAGNNNNNCTC 2 cut(s) 734, 766
BpmI CTGGAG 1 cut(s) 710
BpuEI CTTGAG 1 cut(s) 799
BsaAI YACGTR 1 cut(s) 606
BsaJI CCNNGG 1 cut(s) 365
Bse1I ACTGG 1 cut(s) 727
BseBI CCWGG 2 cut(s) 191, 480
BseDI CCNNGG 1 cut(s) 365
BseGI GGATG 2 cut(s) 227, 292
BseMII CTCAG 2 cut(s) 155, 765
BseNI ACTGG 1 cut(s) 727
BseSI GKGCMC 1 cut(s) 402
BseXI GCAGC 1 cut(s) 742
Bsh1236I CGCG 1 cut(s) 396
BshFI GGCC 3 cut(s) 153, 478, 652
BsiHKAI GWGCWC 3 cut(s) 402, 641, 706
BsiSI CCGG 1 cut(s) 818
BslFI GGGAC 1 cut(s) 405
BsmAI GTCTC 1 cut(s) 449
BsmFI GGGAC 1 cut(s) 405
BsnI GGCC 3 cut(s) 153, 478, 652
Bsp1286I GDGCHC 3 cut(s) 402, 641, 706
Bsp143I GATC 2 cut(s) 538, 543
BspACI CCGC 1 cut(s) 423
BspANI GGCC 3 cut(s) 153, 478, 652
BspCNI CTCAG 2 cut(s) 156, 764
BspFNI CGCG 1 cut(s) 396
BspLI GGNNCC 3 cut(s) 390, 421, 518
BsrI ACTGG 1 cut(s) 727
BssECI CCNNGG 1 cut(s) 365
BssMI GATC 2 cut(s) 538, 543
Bst2UI CCWGG 2 cut(s) 191, 480
Bst4CI ACNGT 1 cut(s) 241
BstBAI YACGTR 1 cut(s) 606
BstC8I GCNNGC 1 cut(s) 398
BstDEI CTNAG 3 cut(s) 164, 275, 751
BstF5I GGATG 2 cut(s) 227, 292
BstFNI CGCG 1 cut(s) 396
BstHHI GCGC 1 cut(s) 799
BstKTI GATC 2 cut(s) 541, 546
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 2 cut(s) 538, 543
BstMWI GCNNNNNNNGC 1 cut(s) 761
BstNI CCWGG 2 cut(s) 191, 480
BstNSI RCATGY 1 cut(s) 514
BstSCI CCNGG 2 cut(s) 189, 478
BstSLI GKGCMC 1 cut(s) 402
BstSNI TACGTA 1 cut(s) 606
BstUI CGCG 1 cut(s) 396
BstV1I GCAGC 1 cut(s) 742
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
BsuI GTATCC 1 cut(s) 618
BsuRI GGCC 3 cut(s) 153, 478, 652
BtgZI GCGATG 2 cut(s) 270, 394
BtsCI GGATG 2 cut(s) 227, 292
BtsIMutI CAGTG 1 cut(s) 53
Cac8I GCNNGC 1 cut(s) 398
CfoI GCGC 1 cut(s) 799
Cfr13I GGNCC 2 cut(s) 388, 419
CviAII CATG 3 cut(s) 499, 511, 803
DdeI CTNAG 3 cut(s) 164, 275, 751
DpnI GATC 2 cut(s) 540, 545
DpnII GATC 2 cut(s) 538, 543
Ecl136II GAGCTC 1 cut(s) 704
Eco105I TACGTA 1 cut(s) 606
Eco147I AGGCCT 1 cut(s) 478
Eco24I GRGCYC 1 cut(s) 706
Eco47I GGWCC 2 cut(s) 388, 419
Eco53kI GAGCTC 1 cut(s) 704
EcoICRI GAGCTC 1 cut(s) 704
EcoO109I RGGNCCY 1 cut(s) 388
EcoRII CCWGG 2 cut(s) 189, 478
EcoT38I GRGCYC 1 cut(s) 706
FaeI CATG 3 cut(s) 502, 514, 806
FaqI GGGAC 1 cut(s) 405
FatI CATG 3 cut(s) 498, 510, 802
FauI CCCGC 1 cut(s) 430
Fnu4HI GCNGC 1 cut(s) 756
FokI GGATG 2 cut(s) 214, 279
FriOI GRGCYC 1 cut(s) 706
Fsp4HI GCNGC 1 cut(s) 756
FspBI CTAG 1 cut(s) 465
GlaI GCGC 1 cut(s) 798
GluI GCNGC 1 cut(s) 756
GsuI CTGGAG 1 cut(s) 710
HaeIII GGCC 3 cut(s) 153, 478, 652
HapII CCGG 1 cut(s) 818
HhaI GCGC 1 cut(s) 799
Hin1II CATG 3 cut(s) 502, 514, 806
Hin6I GCGC 1 cut(s) 797
HinP1I GCGC 1 cut(s) 797
HinfI GANTC 2 cut(s) 116, 806
HpaII CCGG 1 cut(s) 818
HphI GGTGA 3 cut(s) 53, 272, 802
Hpy166II GTNNAC 2 cut(s) 53, 400
Hpy188I TCNGA 3 cut(s) 91, 543, 742
Hpy188III TCNNGA 3 cut(s) 146, 536, 778
Hpy8I GTNNAC 2 cut(s) 53, 400
HpyAV CCTTC 5 cut(s) 306, 413, 692, 777, 780
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 3 cut(s) 185, 400, 748
HpyF10VI GCNNNNNNNGC 1 cut(s) 761
HpyF3I CTNAG 3 cut(s) 164, 275, 751
HpySE526I ACGT 1 cut(s) 605
Hsp92II CATG 3 cut(s) 502, 514, 806
HspAI GCGC 1 cut(s) 797
Kzo9I GATC 2 cut(s) 538, 543
LmnI GCTCC 2 cut(s) 522, 701
LpnPI CCDG 9 cut(s) 24, 173, 176, 203, 230, 465, 492, 740, 831
Lsp1109I GCAGC 1 cut(s) 742
LweI GCATC 1 cut(s) 735
MaeI CTAG 1 cut(s) 465
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 1 cut(s) 494
MalI GATC 2 cut(s) 540, 545
MboI GATC 2 cut(s) 538, 543
MboII GAAGA 1 cut(s) 801
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 3 cut(s) 402, 641, 706
MluCI AATT 2 cut(s) 222, 679
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 8 cut(s) 130, 222, 274, 375, 379, 402, 663, 781
MroXI GAANNNNTTC 1 cut(s) 69
MseI TTAA 1 cut(s) 473
MspA1I CMGCKG 2 cut(s) 163, 755
MspI CCGG 1 cut(s) 818
MspR9I CCNGG 2 cut(s) 191, 480
MvaI CCWGG 2 cut(s) 191, 480
MvnI CGCG 1 cut(s) 396
MwoI GCNNNNNNNGC 1 cut(s) 761
NdeII GATC 2 cut(s) 538, 543
NlaIII CATG 3 cut(s) 502, 514, 806
NlaIV GGNNCC 3 cut(s) 390, 421, 518
NspI RCATGY 1 cut(s) 514
PceI AGGCCT 1 cut(s) 478
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 1 cut(s) 806
PkrI GCNGC 1 cut(s) 757
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Ppu21I YACGTR 1 cut(s) 606
PpuMI RGGWCCY 1 cut(s) 388
Psp124BI GAGCTC 1 cut(s) 706
Psp5II RGGWCCY 1 cut(s) 388
Psp6I CCWGG 2 cut(s) 189, 478
PspGI CCWGG 2 cut(s) 189, 478
PspN4I GGNNCC 3 cut(s) 390, 421, 518
PspPI GGNCC 2 cut(s) 388, 419
PspPPI RGGWCCY 1 cut(s) 388
PsuI RGATCY 1 cut(s) 538
PvuII CAGCTG 2 cut(s) 163, 755
SacI GAGCTC 1 cut(s) 706
SaqAI TTAA 1 cut(s) 473
SatI GCNGC 1 cut(s) 756
Sau3AI GATC 2 cut(s) 538, 543
Sau96I GGNCC 2 cut(s) 388, 419
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 2 cut(s) 191, 480
SduI GDGCHC 3 cut(s) 402, 641, 706
SfaNI GCATC 1 cut(s) 735
SinI GGWCC 2 cut(s) 388, 419
SmlI CTYRAG 1 cut(s) 778
SmoI CTYRAG 1 cut(s) 778
SnaBI TACGTA 1 cut(s) 606
Sse9I AATT 2 cut(s) 222, 679
SseBI AGGCCT 1 cut(s) 478
SsiI CCGC 1 cut(s) 423
SspI AATATT 1 cut(s) 70
SspMI CTAG 1 cut(s) 465
SstI GAGCTC 1 cut(s) 706
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 2 cut(s) 189, 478
TaaI ACNGT 1 cut(s) 241
TaiI ACGT 1 cut(s) 608
TaqI TCGA 1 cut(s) 579
TasI AATT 2 cut(s) 222, 679
TfiI GAWTC 1 cut(s) 806
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TscAI CASTG 1 cut(s) 60
TseI GCWGC 1 cut(s) 755
TspDTI ATGAA 2 cut(s) 192, 819
TspRI CASTG 1 cut(s) 60
VneI GTGCAC 1 cut(s) 398
VpaK11BI GGWCC 2 cut(s) 388, 419
XceI RCATGY 1 cut(s) 514
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.