RLG00000026245

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018087
Physical Location & Seq
Reverse (-)
1550050 .. 1551412
1363 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026245

Sequence Viewer

Length: 651 bp
ATGCAGGTTCTGGTTGTCGGTGGTGGTGATGGCGGAGTTCTTAGGGAGATATCGCGCCATCCTTCTGTTGAGCATATTGATATATGTGAGATAGATAAGATGGTTATAGATGTGTCTAAGAAGTTTTTTCCAGAGTTAGCTGTTGGATTTGAGGACCCTCGTGTCCATCTGCATGTTGGTGATGGTATGCATGCACCTGAAGGGAAGTATGATGCTATAATTGTTGATTCATCAGATCCTGTAGGTCCTGTTCAAGAGCTTGTAGAGAAGCCATTTTTTGAGACAATCGCTAGAGCATTAAGGCCTGGCGGTGTTCTCAGTAACATGGCAGAGAGTATGTGGCTCCATACACATCTTATTGAAGATATGATCTCTGTTTGCCATCTAACATTCAAGGGGTCAGTCAACTATGCATGGGCGAGTGTTCCTACATATCCAAGTGGTGTTATAGGTATTCTGCTATGCTCAACAGAAGGGCCTCCTGTTGATTTTAAAAATCCAGTCAACTCCATTGAGAAGTTGGAAGGAGCTGTCAAACACAGGAGAACTTCGATGCACTCAGCTGCCTTTGCCTTGCCTGCTTTTTTGATGAAGGAGGTGAGCGCACTCCGTGATTCTTCAACACCAGCAAGACGAAGCGGCTTGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

217

Amino Acids

23.48

Weight (kDa)

5.78

Isoelectric Point (pI)

57.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 2 - 166 1.4e-55 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018087)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g31860
malus_domestica MD07G1292400.v1.1
prunus_persica Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1
pyrus_communis pycom01g23060
rosa_laevigata RLG00000026245
rosa_rugosa Rorug01G0427500 Rorug01G0427700
rosa_samantha Rh1AG450700 Rh1BG406900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AccII CGCG 1 cut(s) 55
AciI CCGC 3 cut(s) 33, 309, 639
AclWI GGATC 1 cut(s) 230
AcuI CTGAAG 1 cut(s) 219
AdeI CACNNNGTG 1 cut(s) 611
AgsI TTSAA 4 cut(s) 254, 362, 394, 621
AjnI CCWGG 1 cut(s) 304
AluBI AGCT 4 cut(s) 140, 259, 530, 563
AluI AGCT 4 cut(s) 140, 259, 530, 563
Alw26I GTCTC 1 cut(s) 275
AlwI GGATC 1 cut(s) 230
AlwNI CAGNNNCTG 2 cut(s) 10, 239
AoxI GGCC 2 cut(s) 302, 476
ApeKI GCWGC 1 cut(s) 563
AspLEI GCGC 2 cut(s) 57, 605
AspS9I GGNCC 3 cut(s) 154, 245, 476
AsuHPI GGTGA 3 cut(s) 38, 191, 610
AvaII GGWCC 2 cut(s) 154, 245
BauI CACGAG 1 cut(s) 159
BbvI GCAGC 1 cut(s) 550
BccI CCATC 6 cut(s) 23, 66, 94, 174, 176, 390
BciT130I CCWGG 1 cut(s) 306
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 2 cut(s) 291, 649
BfmI CTRYAG 1 cut(s) 240
BisI GCNGC 2 cut(s) 564, 640
BlsI GCNGC 2 cut(s) 565, 641
Bme1390I CCNGG 1 cut(s) 306
Bme18I GGWCC 2 cut(s) 154, 245
BmgT120I GGNCC 3 cut(s) 154, 245, 476
BmiI GGNNCC 2 cut(s) 156, 344
BmrFI CCNGG 1 cut(s) 306
BmsI GCATC 2 cut(s) 202, 543
Bse1I ACTGG 1 cut(s) 500
BseBI CCWGG 1 cut(s) 306
BseGI GGATG 1 cut(s) 58
BseMII CTCAG 2 cut(s) 331, 573
BseNI ACTGG 1 cut(s) 500
BseXI GCAGC 1 cut(s) 550
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 2 cut(s) 304, 478
BsmAI GTCTC 1 cut(s) 275
BsnI GGCC 2 cut(s) 304, 478
Bsp143I GATC 2 cut(s) 235, 369
BspACI CCGC 3 cut(s) 33, 309, 639
BspANI GGCC 2 cut(s) 304, 478
BspCNI CTCAG 2 cut(s) 330, 572
BspFNI CGCG 1 cut(s) 55
BspLI GGNNCC 2 cut(s) 156, 344
BspPI GGATC 1 cut(s) 230
BsrI ACTGG 1 cut(s) 500
BssMI GATC 2 cut(s) 235, 369
BssSI CACGAG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 192, 579
BstDEI CTNAG 4 cut(s) 41, 117, 317, 559
BstF5I GGATG 1 cut(s) 58
BstFNI CGCG 1 cut(s) 55
BstHHI GCGC 2 cut(s) 57, 605
BstKTI GATC 2 cut(s) 238, 372
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 2 cut(s) 235, 369
BstMWI GCNNNNNNNGC 2 cut(s) 569, 578
BstNI CCWGG 1 cut(s) 306
BstNSI RCATGY 2 cut(s) 176, 194
BstSCI CCNGG 1 cut(s) 304
BstSFI CTRYAG 1 cut(s) 240
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 550
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BsuRI GGCC 2 cut(s) 304, 478
BtsCI GGATG 1 cut(s) 58
Cac8I GCNNGC 2 cut(s) 192, 579
CaiI CAGNNNCTG 2 cut(s) 10, 239
CfoI GCGC 2 cut(s) 57, 605
Cfr13I GGNCC 3 cut(s) 154, 245, 476
CviAII CATG 4 cut(s) 173, 191, 325, 414
CviJI RGCY 9 cut(s) 140, 259, 271, 304, 343, 478, 530, 563, 642
CviKI_1 RGCY 9 cut(s) 140, 259, 271, 304, 343, 478, 530, 563, 642
DdeI CTNAG 4 cut(s) 41, 117, 317, 559
DpnI GATC 2 cut(s) 237, 371
DpnII GATC 2 cut(s) 235, 369
DraI TTTAAA 1 cut(s) 493
DraIII CACNNNGTG 1 cut(s) 611
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EciI GGCGGA 1 cut(s) 48
Eco147I AGGCCT 1 cut(s) 304
Eco32I GATATC 1 cut(s) 51
Eco47I GGWCC 2 cut(s) 154, 245
Eco57I CTGAAG 1 cut(s) 219
EcoO109I RGGNCCY 3 cut(s) 154, 245, 476
EcoRII CCWGG 1 cut(s) 304
EcoRV GATATC 1 cut(s) 51
EcoT22I ATGCAT 2 cut(s) 192, 415
FaeI CATG 4 cut(s) 176, 194, 328, 417
FatI CATG 4 cut(s) 172, 190, 324, 413
Fnu4HI GCNGC 2 cut(s) 564, 640
FokI GGATG 1 cut(s) 45
Fsp4HI GCNGC 2 cut(s) 564, 640
FspBI CTAG 2 cut(s) 291, 649
GlaI GCGC 2 cut(s) 56, 604
GluI GCNGC 2 cut(s) 564, 640
HaeIII GGCC 2 cut(s) 304, 478
HhaI GCGC 2 cut(s) 57, 605
Hin1II CATG 4 cut(s) 176, 194, 328, 417
Hin6I GCGC 2 cut(s) 55, 603
HinP1I GCGC 2 cut(s) 55, 603
HincII GTYRAC 2 cut(s) 406, 505
HindII GTYRAC 2 cut(s) 406, 505
HinfI GANTC 2 cut(s) 227, 614
HphI GGTGA 3 cut(s) 38, 191, 610
Hpy166II GTNNAC 2 cut(s) 406, 505
Hpy188I TCNGA 1 cut(s) 235
Hpy188III TCNNGA 2 cut(s) 131, 254
Hpy8I GTNNAC 2 cut(s) 406, 505
HpyAV CCTTC 5 cut(s) 72, 194, 467, 518, 586
HpyCH4V TGCA 6 cut(s) 4, 172, 190, 194, 413, 556
HpyF10VI GCNNNNNNNGC 2 cut(s) 569, 578
HpyF3I CTNAG 4 cut(s) 41, 117, 317, 559
Hsp92II CATG 4 cut(s) 176, 194, 328, 417
HspAI GCGC 2 cut(s) 55, 603
Kzo9I GATC 2 cut(s) 235, 369
LmnI GCTCC 2 cut(s) 348, 527
Lsp1109I GCAGC 1 cut(s) 550
LweI GCATC 2 cut(s) 202, 543
MaeI CTAG 2 cut(s) 291, 649
MaeIII GTNAC 1 cut(s) 320
MalI GATC 2 cut(s) 237, 371
MboI GATC 2 cut(s) 235, 369
MboII GAAGA 2 cut(s) 374, 609
MflI RGATCY 1 cut(s) 235
MluCI AATT 1 cut(s) 219
MmeI TCCRAC 2 cut(s) 124, 501
MnlI CCTC 4 cut(s) 145, 168, 489, 589
Mph1103I ATGCAT 2 cut(s) 192, 415
MseI TTAA 2 cut(s) 299, 492
MslI CAYNNNNRTG 2 cut(s) 171, 177
MspA1I CMGCKG 1 cut(s) 563
MspR9I CCNGG 1 cut(s) 306
MvaI CCWGG 1 cut(s) 306
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 2 cut(s) 569, 578
NdeII GATC 2 cut(s) 235, 369
NlaIII CATG 4 cut(s) 176, 194, 328, 417
NlaIV GGNNCC 2 cut(s) 156, 344
NsiI ATGCAT 2 cut(s) 192, 415
NspI RCATGY 2 cut(s) 176, 194
PaeI GCATGC 1 cut(s) 194
PceI AGGCCT 1 cut(s) 304
PfeI GAWTC 2 cut(s) 227, 614
PkrI GCNGC 2 cut(s) 565, 641
PpuMI RGGWCCY 2 cut(s) 154, 245
Psp5II RGGWCCY 2 cut(s) 154, 245
Psp6I CCWGG 1 cut(s) 304
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 2 cut(s) 156, 344
PspPI GGNCC 3 cut(s) 154, 245, 476
PspPPI RGGWCCY 2 cut(s) 154, 245
PsrI GAACNNNNNNTAC 2 cut(s) 234, 266
PstNI CAGNNNCTG 2 cut(s) 10, 239
PsuI RGATCY 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 563
RseI CAYNNNNRTG 2 cut(s) 171, 177
SaqAI TTAA 2 cut(s) 299, 492
SatI GCNGC 2 cut(s) 564, 640
Sau3AI GATC 2 cut(s) 235, 369
Sau96I GGNCC 3 cut(s) 154, 245, 476
ScrFI CCNGG 1 cut(s) 306
SetI ASST 9 cut(s) 9, 142, 199, 247, 261, 454, 532, 565, 600
SfaNI GCATC 2 cut(s) 202, 543
SfcI CTRYAG 1 cut(s) 240
SinI GGWCC 2 cut(s) 154, 245
SmiMI CAYNNNNRTG 2 cut(s) 171, 177
SphI GCATGC 1 cut(s) 194
Sse9I AATT 1 cut(s) 219
SseBI AGGCCT 1 cut(s) 304
SsiI CCGC 3 cut(s) 33, 309, 639
SspMI CTAG 2 cut(s) 291, 649
StuI AGGCCT 1 cut(s) 304
StyD4I CCNGG 1 cut(s) 304
TaqI TCGA 1 cut(s) 551
TasI AATT 1 cut(s) 219
TauI GCSGC 1 cut(s) 642
TfiI GAWTC 2 cut(s) 227, 614
Tru1I TTAA 2 cut(s) 299, 492
Tru9I TTAA 2 cut(s) 299, 492
TseI GCWGC 1 cut(s) 563
TspDTI ATGAA 2 cut(s) 219, 605
TspGWI ACGGA 1 cut(s) 599
VpaK11BI GGWCC 2 cut(s) 154, 245
XceI RCATGY 2 cut(s) 176, 194
XcmI CCANNNNNNNNNTGG 2 cut(s) 173, 517
XspI CTAG 2 cut(s) 291, 649
Zsp2I ATGCAT 2 cut(s) 192, 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.