Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0010316

Physical Location & Seq

Genomic Coordinates Reverse (-)

35657231 .. 35661208

Gene Structure 3978 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1296100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 906 bp

ATGAATTACCTAATTGGAGCATTCAAACCATCCTGCAACATTTCAATCACATTTTCTGATGCCAAAACTCGAAAGCAGGTCCCTTTAAAGAAGGAAAGTGGCCAAGCAGTATTGGTCCCACTTTTTCAAAGTCAAGAAAACATTTTTGGGAAGATTAATATAGAACCAATTCAAGGGAAGAAGGTCGAACACAATGGAGTTAAAGTCGAGCTTCTCGGTCAAATCGAGATGTACTTTGACAGAGGCAACTTTTATGACTTTACTTCTCTTGTTCGTGAACTTGATGTTCCTGGAGAGATATATGAAAGGAAAACATATCCTTTTGAGTTTTCTACAGTTGAGATGCCATATGAGACTTACAACGGGGTGAATGTGAGACTTAGGTATGTTCTGAAAGTCACAATTAGTCGTGGTTATGGTAGTAGCATAGTGGAATACCAGGATTTTGTGGTTCGCAACTATTCCCCACCTCCATCTATTAACAATAGCATCAAGATGGAAGTCGGAATCGAAGATTGTCTGCACATTGAGTTTGAGTACAACAAGAGCAAGTACCATCTGAAAGACGTTATTATTGGCAAGATATATTTTCTTCTTGTGAGAATCAAGATAAAGAATATGGATCTGGAGATCAGGCGTCGAGAGTCAACAGGGTCAGGGCCCAACACCCATGTTGAGACAGAGACACTTGCTAAGTTTGAGTTGATGGATGGTGCTCCAGTAAGAGGTGAATCAATTCCCATTAGATTGTTTCTTAGCCCTTATGAACTCACACCAACACACCGCAACATCAATAACAAATTCAGCGTGAAGTATTATTTGAATCTTGTTCTGGTTGATGAAGAGGACCGACGGTATTTCAAGCAGCAAGAAATCACCATATACAGGCTTCAAGAGACGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008565 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0016197 GO:0016482 GO:0030904 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042147 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098796

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Vps26

Protein Analysis

302

Amino Acids

35.08

Weight (kDa)

7.68

Isoelectric Point (pI)

52.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps26	PF03643	8 - 283	9.3e-131	Vacuolar protein sorting-associated protein 26

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010316)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27690 AT4G27690 AT5G53530
fragaria_vesca	FvH4_7g32410
malus_domestica	MD01G1225600.v1.1 MD07G1296100.v1.1
prunus_persica	Prupe.2G315700_v2.0.a1
pyrus_communis	pycom01g23400
rosa_chinensis	RchiOBHm_Chr1g0381991
rosa_laevigata	RLG00000026195
rosa_multiflora	Rmu_co8508943.1_g000001 Rmu_sc0000110.1_g000002 Rmu_sc0009991.1_g000002
rosa_roxburghii	Rroxscaffold_4G00278280
rosa_rugosa	Rorug01G0432700
rosa_samantha	Rh1AG456000 Rh1BG413100 Rh1CG428000 Rh1DG445400
rosa_wichuraiana	Rw1G041320 Rw1G041530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	902
Acc36I	ACCTGC	1 cut(s)	67
AciI	CCGC	1 cut(s)	784
AclWI	GGATC	1 cut(s)	630
AcoI	YGGCCR	1 cut(s)	100
AcsI	RAATTY	1 cut(s)	800
AcyI	GRCGYC	2 cut(s)	637, 899
AfaI	GTAC	3 cut(s)	233, 539, 554
AfiI	CCNNNNNNNGG	3 cut(s)	173, 725, 885
AgsI	TTSAA	7 cut(s)	25, 45, 128, 173, 823, 862, 893
AjnI	CCWGG	2 cut(s)	289, 438
AjuI	GAANNNNNNNTTGG	2 cut(s)	129, 161
AluBI	AGCT	1 cut(s)	211
AluI	AGCT	1 cut(s)	211
Alw21I	GWGCWC	1 cut(s)	718
Alw26I	GTCTC	5 cut(s)	347, 370, 671, 677, 890
AlwI	GGATC	1 cut(s)	630
AoxI	GGCC	2 cut(s)	100, 659
ApaI	GGGCCC	1 cut(s)	663
ApeKI	GCWGC	1 cut(s)	865
ApoI	RAATTY	1 cut(s)	800
AseI	ATTAAT	1 cut(s)	156
Asp700I	GAANNNNTTC	2 cut(s)	168, 735
AspS9I	GGNCC	5 cut(s)	79, 115, 659, 660, 847
AsuHPI	GGTGA	3 cut(s)	379, 740, 868
AvaII	GGWCC	3 cut(s)	79, 115, 847
BaeGI	GKGCMC	1 cut(s)	663
BalI	TGGCCA	1 cut(s)	102
BanII	GRGCYC	1 cut(s)	663
Bbv12I	GWGCWC	1 cut(s)	718
BbvI	GCAGC	1 cut(s)	877
BccI	CCATC	6 cut(s)	37, 481, 490, 564, 700, 704
BciT130I	CCWGG	2 cut(s)	291, 440
BcoDI	GTCTC	5 cut(s)	347, 370, 671, 677, 890
BfmI	CTRYAG	1 cut(s)	333
BfuAI	ACCTGC	1 cut(s)	67
BisI	GCNGC	1 cut(s)	866
BlsI	GCNGC	1 cut(s)	867
Bme1390I	CCNGG	2 cut(s)	291, 440
Bme18I	GGWCC	3 cut(s)	79, 115, 847
BmgT120I	GGNCC	5 cut(s)	79, 115, 659, 660, 847
BmiI	GGNNCC	3 cut(s)	81, 117, 661
BmrFI	CCNGG	2 cut(s)	291, 440
BmsI	GCATC	3 cut(s)	49, 333, 498
BpmI	CTGGAG	3 cut(s)	312, 647, 702
BsaHI	GRCGYC	2 cut(s)	637, 899
BsaXI	ACNNNNNCTCC	2 cut(s)	189, 219
Bsc4I	CCNNNNNNNGG	3 cut(s)	173, 725, 885
Bse1I	ACTGG	1 cut(s)	719
BseBI	CCWGG	2 cut(s)	291, 440
BseGI	GGATG	2 cut(s)	29, 715
BseLI	CCNNNNNNNGG	3 cut(s)	173, 725, 885
BseNI	ACTGG	1 cut(s)	719
BseSI	GKGCMC	1 cut(s)	663
BseXI	GCAGC	1 cut(s)	877
BsgI	GTGCAG	1 cut(s)	506
BshFI	GGCC	2 cut(s)	102, 661
BsiHKAI	GWGCWC	1 cut(s)	718
BslFI	GGGAC	2 cut(s)	65, 101
BslI	CCNNNNNNNGG	3 cut(s)	173, 725, 885
BsmAI	GTCTC	5 cut(s)	347, 370, 671, 677, 890
BsmBI	CGTCTC	1 cut(s)	890
BsmFI	GGGAC	2 cut(s)	65, 101
BsmI	GAATGC	1 cut(s)	20
BsnI	GGCC	2 cut(s)	102, 661
Bsp120I	GGGCCC	1 cut(s)	659
Bsp1286I	GDGCHC	2 cut(s)	663, 718
Bsp143I	GATC	2 cut(s)	622, 630
BspACI	CCGC	1 cut(s)	784
BspANI	GGCC	2 cut(s)	102, 661
BspHI	TCATGA	1 cut(s)	902
BspLI	GGNNCC	3 cut(s)	81, 117, 661
BspMI	ACCTGC	1 cut(s)	67
BspPI	GGATC	1 cut(s)	630
BsrI	ACTGG	1 cut(s)	719
BssMI	GATC	2 cut(s)	622, 630
BssNI	GRCGYC	2 cut(s)	637, 899
Bst2UI	CCWGG	2 cut(s)	291, 440
Bst4CI	ACNGT	2 cut(s)	337, 855
Bst6I	CTCTTC	1 cut(s)	837
BstACI	GRCGYC	2 cut(s)	637, 899
BstDEI	CTNAG	3 cut(s)	380, 693, 755
BstF5I	GGATG	2 cut(s)	29, 715
BstKTI	GATC	2 cut(s)	625, 633
BstMAI	GTCTC	5 cut(s)	347, 370, 671, 677, 890
BstMBI	GATC	2 cut(s)	622, 630
BstNI	CCWGG	2 cut(s)	291, 440
BstSCI	CCNGG	2 cut(s)	289, 438
BstSFI	CTRYAG	1 cut(s)	333
BstSLI	GKGCMC	1 cut(s)	663
BstV1I	GCAGC	1 cut(s)	877
BstX2I	RGATCY	1 cut(s)	622
BstYI	RGATCY	1 cut(s)	622
BsuRI	GGCC	2 cut(s)	102, 661
BtsCI	GGATG	2 cut(s)	29, 715
BveI	ACCTGC	1 cut(s)	67
CciI	TCATGA	1 cut(s)	902
Cfr13I	GGNCC	5 cut(s)	79, 115, 659, 660, 847
CseI	GACGC	1 cut(s)	626
Csp6I	GTAC	3 cut(s)	232, 538, 553
CviAII	CATG	2 cut(s)	671, 903
CviJI	RGCY	5 cut(s)	102, 211, 661, 759, 889
CviKI_1	RGCY	5 cut(s)	102, 211, 661, 759, 889
CviQI	GTAC	3 cut(s)	232, 538, 553
DdeI	CTNAG	3 cut(s)	380, 693, 755
DpnI	GATC	2 cut(s)	624, 632
DpnII	GATC	2 cut(s)	622, 630
DraI	TTTAAA	1 cut(s)	87
EaeI	YGGCCR	1 cut(s)	100
Eam1104I	CTCTTC	1 cut(s)	837
EarI	CTCTTC	1 cut(s)	837
Eco24I	GRGCYC	1 cut(s)	663
Eco47I	GGWCC	3 cut(s)	79, 115, 847
EcoO109I	RGGNCCY	2 cut(s)	79, 659
EcoRII	CCWGG	2 cut(s)	289, 438
EcoT38I	GRGCYC	1 cut(s)	663
Esp3I	CGTCTC	1 cut(s)	890
FaeI	CATG	2 cut(s)	674, 906
FalI	AAGNNNNNCTT	2 cut(s)	195, 227
FaqI	GGGAC	2 cut(s)	65, 101
FatI	CATG	2 cut(s)	670, 902
FauNDI	CATATG	1 cut(s)	349
Fnu4HI	GCNGC	1 cut(s)	866
FokI	GGATG	2 cut(s)	16, 722
FriOI	GRGCYC	1 cut(s)	663
Fsp4HI	GCNGC	1 cut(s)	866
GluI	GCNGC	1 cut(s)	866
GsuI	CTGGAG	3 cut(s)	312, 647, 702
HaeIII	GGCC	2 cut(s)	102, 661
HgaI	GACGC	1 cut(s)	626
Hin1I	GRCGYC	2 cut(s)	637, 899
Hin1II	CATG	2 cut(s)	674, 906
HincII	GTYRAC	1 cut(s)	648
HindII	GTYRAC	1 cut(s)	648
HinfI	GANTC	5 cut(s)	507, 603, 644, 731, 823
HphI	GGTGA	3 cut(s)	379, 740, 868
Hpy166II	GTNNAC	2 cut(s)	278, 648
Hpy188I	TCNGA	4 cut(s)	58, 393, 506, 561
Hpy188III	TCNNGA	9 cut(s)	134, 226, 275, 493, 607, 626, 641, 893, 903
Hpy8I	GTNNAC	2 cut(s)	278, 648
Hpy99I	CGWCG	2 cut(s)	642, 855
HpyAV	CCTTC	2 cut(s)	85, 175
HpyCH4III	ACNGT	2 cut(s)	337, 855
HpyCH4IV	ACGT	2 cut(s)	567, 899
HpyCH4V	TGCA	2 cut(s)	36, 523
HpyF3I	CTNAG	3 cut(s)	380, 693, 755
HpySE526I	ACGT	2 cut(s)	567, 899
Hsp92I	GRCGYC	2 cut(s)	637, 899
Hsp92II	CATG	2 cut(s)	674, 906
Kzo9I	GATC	2 cut(s)	622, 630
LmnI	GCTCC	2 cut(s)	17, 721
Lsp1109I	GCAGC	1 cut(s)	877
LweI	GCATC	3 cut(s)	49, 333, 498
MaeII	ACGT	2 cut(s)	567, 899
MaeIII	GTNAC	1 cut(s)	397
MalI	GATC	2 cut(s)	624, 632
MboI	GATC	2 cut(s)	622, 630
MboII	GAAGA	5 cut(s)	163, 190, 524, 584, 854
MflI	RGATCY	1 cut(s)	622
MhlI	GDGCHC	2 cut(s)	663, 718
MlsI	TGGCCA	1 cut(s)	102
MluCI	AATT	6 cut(s)	4, 12, 168, 402, 735, 800
MluNI	TGGCCA	1 cut(s)	102
MlyI	GAGTC	1 cut(s)	653
MmeI	TCCRAC	1 cut(s)	484
MnlI	CCTC	4 cut(s)	236, 480, 719, 838
Mox20I	TGGCCA	1 cut(s)	102
MroXI	GAANNNNTTC	2 cut(s)	168, 735
MscI	TGGCCA	1 cut(s)	102
MseI	TTAA	4 cut(s)	86, 156, 201, 480
MslI	CAYNNNNRTG	1 cut(s)	494
Msp20I	TGGCCA	1 cut(s)	102
MspR9I	CCNGG	2 cut(s)	291, 440
Mva1269I	GAATGC	1 cut(s)	20
MvaI	CCWGG	2 cut(s)	291, 440
NdeI	CATATG	1 cut(s)	349
NdeII	GATC	2 cut(s)	622, 630
NlaIII	CATG	2 cut(s)	674, 906
NlaIV	GGNNCC	3 cut(s)	81, 117, 661
NmuCI	GTSAC	1 cut(s)	397
PagI	TCATGA	1 cut(s)	902
PcsI	WCGNNNNNNNCGW	1 cut(s)	222
PctI	GAATGC	1 cut(s)	20
PdmI	GAANNNNTTC	2 cut(s)	168, 735
PfeI	GAWTC	4 cut(s)	507, 603, 731, 823
PfoI	TCCNGGA	1 cut(s)	289
PkrI	GCNGC	1 cut(s)	867
PleI	GAGTC	1 cut(s)	652
PpsI	GAGTC	1 cut(s)	652
PpuMI	RGGWCCY	1 cut(s)	79
PshBI	ATTAAT	1 cut(s)	156
Psp5II	RGGWCCY	1 cut(s)	79
Psp6I	CCWGG	2 cut(s)	289, 438
PspGI	CCWGG	2 cut(s)	289, 438
PspN4I	GGNNCC	3 cut(s)	81, 117, 661
PspOMI	GGGCCC	1 cut(s)	659
PspPI	GGNCC	5 cut(s)	79, 115, 659, 660, 847
PspPPI	RGGWCCY	1 cut(s)	79
PsuI	RGATCY	1 cut(s)	622
RsaI	GTAC	3 cut(s)	233, 539, 554
RsaNI	GTAC	3 cut(s)	232, 538, 553
RseI	CAYNNNNRTG	1 cut(s)	494
SaqAI	TTAA	4 cut(s)	86, 156, 201, 480
SatI	GCNGC	1 cut(s)	866
Sau3AI	GATC	2 cut(s)	622, 630
Sau96I	GGNCC	5 cut(s)	79, 115, 659, 660, 847
SchI	GAGTC	1 cut(s)	653
ScrFI	CCNGG	2 cut(s)	291, 440
SduI	GDGCHC	2 cut(s)	663, 718
SetI	ASST	9 cut(s)	12, 81, 186, 213, 386, 472, 570, 730, 902
SfaNI	GCATC	3 cut(s)	49, 333, 498
SfcI	CTRYAG	1 cut(s)	333
SinI	GGWCC	3 cut(s)	79, 115, 847
SmiMI	CAYNNNNRTG	1 cut(s)	494
Sse9I	AATT	6 cut(s)	4, 12, 168, 402, 735, 800
SsiI	CCGC	1 cut(s)	784
StyD4I	CCNGG	2 cut(s)	289, 438
TaaI	ACNGT	2 cut(s)	337, 855
TaiI	ACGT	2 cut(s)	570, 902
TaqI	TCGA	6 cut(s)	70, 186, 207, 225, 510, 640
TaqII	GACCGA	2 cut(s)	206, 864
TasI	AATT	6 cut(s)	4, 12, 168, 402, 735, 800
TatI	WGTACW	2 cut(s)	231, 537
TfiI	GAWTC	4 cut(s)	507, 603, 731, 823
Tru1I	TTAA	4 cut(s)	86, 156, 201, 480
Tru9I	TTAA	4 cut(s)	86, 156, 201, 480
TseFI	GTSAC	1 cut(s)	397
TseI	GCWGC	1 cut(s)	865
Tsp45I	GTSAC	1 cut(s)	397
TspDTI	ATGAA	4 cut(s)	17, 318, 780, 855
VpaK11BI	GGWCC	3 cut(s)	79, 115, 847
VspI	ATTAAT	1 cut(s)	156
XapI	RAATTY	1 cut(s)	800
XmnI	GAANNNNTTC	2 cut(s)	168, 735
ZraI	GACGTC	1 cut(s)	900

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.