Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0015179

Physical Location & Seq

Genomic Coordinates Forward (+)

467294 .. 470555

Gene Structure 3262 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1005200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 921 bp

ATGGCGTCCGAGTTCGATCCCGCTCGTATCGAATCTCACAAATTCCCCGAGCGCACGTACACCTACTCCGAGAGAGATGTAGCGCTCTATGCGTTGGGCATCGGAGCTTGCGCTCGGGATGCCGTGGATGTCGACCAGCTGAAATATGTTTACCATGAAAAGGGCCAGAATTTCATCCAGGTCTTGCCGACATTTTCTGCTCTATTCTCGCTGGGATCTCTGCCAGGTGGTGCTGGTCTTCCAGGGTTGCAATATGATCCGCGACTCTTGCTGCATGGACAACAATACATAGAACTCTACAAGCCACTGCCTTCAAGTGCTGTTTTAAAAAATACTGTGACTCTTGCTGGACTCCATGATAAAGGTAAAGCAGCTATTCTCGAGATTGAAACAAAAAGTTACGACAAAGATTCTGGTGTACTGTATTGCATGAATCGGTCGACTGTTTTTCTACGAGGTGCTGGTGGCTTCTCAAAATCATCCCAGCTGTATTCGTATTCAACCTATCCAAAAGACCAGGTTGCATCTGTGACAACCCCAAAAGGTCAACCTTTTGCAGTGTATGAAGATTGTACACAGCCATCTCAGGCATTGCTATATAGGCTATCTGGAGATTACAATCCTCTGCATTCAGATCCTATGTTCGCAAAAGCTGCAGGGTTCACACGGCCGATACTGCATGGCTTGTGCACACTTGGATTTGCGGTCAGGGCAATTGTGAAATGCATTTGCGGAGAAGATCCAAACCTGGTCAAGTGTGTATCAGGCCGGTTCCTGTTACATGTCTACCCTGGTGAAACTTTGCTCACTGAAATGTGGCTCGAAGGCCTAAGGGTGATATTTCAAACAAAGGTGAAAGAACGAAGCCGGGCGGTGCTCTCTGGCTATGTGGATCTACATGGTTTATCTTCGCGCCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0016042 GO:0016054 GO:0016829 GO:0016835 GO:0016836 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0033542 GO:0034440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0055114 GO:0071704 GO:0072329 GO:0080023 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MaoC_dehydrat_N MaoC_dehydratas

Protein Analysis

307

Amino Acids

33.75

Weight (kDa)

8.36

Isoelectric Point (pI)

37.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFE-2_hydrat-2_N	PF22622	20 - 153	5.6e-37	MFE-2 hydratase 2 N-terminal domain
MaoC_dehydratas	PF01575	180 - 290	6e-26	MaoC like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015179)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76150
fragaria_vesca	FvH4_2g37800 FvH4_2g37800
malus_domestica	MD08G1005200.v1.1
prunus_persica	Prupe.1G357300_v2.0.a1 Prupe.1G357300_v2.0.a1
pyrus_communis	pycom08g00420
rosa_chinensis	RchiOBHm_Chr6g0303911
rosa_laevigata	RLG00000011035
rosa_roxburghii	Rroxscaffold_7G00164270
rosa_rugosa	Rorug06G0325900
rosa_samantha	Rh6AG440100 Rh6BG462900 Rh6CG452400 Rh6DG439400
rosa_wichuraiana	Rw6G038110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	23
AccI	GTMKAC	3 cut(s)	132, 440, 786
AccII	CGCG	2 cut(s)	262, 913
AciI	CCGC	5 cut(s)	21, 260, 704, 732, 872
AclWI	GGATC	6 cut(s)	11, 223, 251, 629, 734, 900
AcoI	YGGCCR	1 cut(s)	668
AcsI	RAATTY	2 cut(s)	41, 169
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	3 cut(s)	59, 420, 574
AfeI	AGCGCT	1 cut(s)	84
AfiI	CCNNNNNNNGG	1 cut(s)	160
AflIII	ACRYGT	1 cut(s)	781
AgsI	TTSAA	4 cut(s)	315, 389, 501, 845
AjnI	CCWGG	6 cut(s)	177, 223, 241, 516, 747, 790
AluBI	AGCT	5 cut(s)	107, 139, 374, 487, 653
AluI	AGCT	5 cut(s)	107, 139, 374, 487, 653
Alw21I	GWGCWC	2 cut(s)	692, 879
Alw44I	GTGCAC	1 cut(s)	688
AlwI	GGATC	6 cut(s)	11, 223, 251, 629, 734, 900
Ama87I	CYCGRG	3 cut(s)	47, 114, 380
Aor51HI	AGCGCT	1 cut(s)	84
AoxI	GGCC	4 cut(s)	163, 668, 766, 826
ApaLI	GTGCAC	1 cut(s)	688
ApeKI	GCWGC	3 cut(s)	271, 371, 653
ApoI	RAATTY	2 cut(s)	41, 169
AspLEI	GCGC	4 cut(s)	54, 85, 113, 915
AspS9I	GGNCC	1 cut(s)	163
AsuC2I	CCSGG	1 cut(s)	869
AsuHPI	GGTGA	3 cut(s)	806, 847, 865
AvaI	CYCGRG	3 cut(s)	47, 114, 380
AxyI	CCTNAGG	1 cut(s)	830
BaeGI	GKGCMC	1 cut(s)	692
BbsI	GAAGAC	1 cut(s)	230
Bbv12I	GWGCWC	2 cut(s)	692, 879
BbvI	GCAGC	3 cut(s)	258, 383, 640
BccI	CCATC	1 cut(s)	589
BceAI	ACGGC	2 cut(s)	107, 683
BciT130I	CCWGG	6 cut(s)	179, 225, 243, 518, 749, 792
BcnI	CCSGG	1 cut(s)	869
BfmI	CTRYAG	2 cut(s)	654, 917
BfoI	RGCGCY	1 cut(s)	86
BisI	GCNGC	3 cut(s)	272, 372, 654
BlsI	GCNGC	3 cut(s)	273, 373, 655
Bme1390I	CCNGG	7 cut(s)	179, 225, 243, 518, 749, 792, 869
BmeT110I	CYCGRG	3 cut(s)	47, 114, 380
BmgT120I	GGNCC	1 cut(s)	163
BmiI	GGNNCC	1 cut(s)	773
BmrFI	CCNGG	7 cut(s)	179, 225, 243, 518, 749, 792, 869
BmsI	GCATC	3 cut(s)	108, 109, 533
BpiI	GAAGAC	1 cut(s)	230
BpmI	CTGGAG	1 cut(s)	630
BpuMI	CCSGG	1 cut(s)	869
BsaAI	YACGTR	1 cut(s)	57
BsaBI	GATNNNNATC	1 cut(s)	618
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	3 cut(s)	123, 242, 790
BsaXI	ACNNNNNCTCC	2 cut(s)	50, 80
Bsc4I	CCNNNNNNNGG	1 cut(s)	160
Bse118I	RCCGGY	1 cut(s)	768
Bse21I	CCTNAGG	1 cut(s)	830
Bse3DI	GCAATG	1 cut(s)	590
Bse8I	GATNNNNATC	1 cut(s)	618
BseBI	CCWGG	6 cut(s)	179, 225, 243, 518, 749, 792
BseDI	CCNNGG	3 cut(s)	123, 242, 790
BseGI	GGATG	4 cut(s)	124, 133, 174, 479
BseJI	GATNNNNATC	1 cut(s)	618
BseLI	CCNNNNNNNGG	1 cut(s)	160
BseMI	GCAATG	1 cut(s)	590
BseMII	CTCAG	1 cut(s)	599
BseSI	GKGCMC	1 cut(s)	692
BseX3I	CGGCCG	1 cut(s)	668
BseXI	GCAGC	3 cut(s)	258, 383, 640
BseYI	CCCAGC	2 cut(s)	211, 483
Bsh1236I	CGCG	2 cut(s)	262, 913
Bsh1285I	CGRYCG	2 cut(s)	440, 671
BshFI	GGCC	4 cut(s)	165, 670, 768, 828
BsiEI	CGRYCG	2 cut(s)	440, 671
BsiHKAI	GWGCWC	2 cut(s)	692, 879
BsiHKCI	CYCGRG	3 cut(s)	47, 114, 380
BsiSI	CCGG	2 cut(s)	769, 868
BslI	CCNNNNNNNGG	1 cut(s)	160
BsmI	GAATGC	1 cut(s)	628
BsnI	GGCC	4 cut(s)	165, 670, 768, 828
BsoBI	CYCGRG	3 cut(s)	47, 114, 380
Bsp1286I	GDGCHC	2 cut(s)	692, 879
Bsp1407I	TGTACA	1 cut(s)	572
Bsp143I	GATC	6 cut(s)	16, 215, 256, 634, 739, 892
BspACI	CCGC	5 cut(s)	21, 260, 704, 732, 872
BspANI	GGCC	4 cut(s)	165, 670, 768, 828
BspCNI	CTCAG	1 cut(s)	598
BspFNI	CGCG	2 cut(s)	262, 913
BspLI	GGNNCC	1 cut(s)	773
BspMAI	CTGCAG	1 cut(s)	658
BspPI	GGATC	6 cut(s)	11, 223, 251, 629, 734, 900
BsrBI	CCGCTC	1 cut(s)	23
BsrDI	GCAATG	1 cut(s)	590
BsrFI	RCCGGY	1 cut(s)	768
BsrGI	TGTACA	1 cut(s)	572
BssAI	RCCGGY	1 cut(s)	768
BssECI	CCNNGG	3 cut(s)	123, 242, 790
BssMI	GATC	6 cut(s)	16, 215, 256, 634, 739, 892
BssNI	GRCGYC	1 cut(s)	5
Bst2UI	CCWGG	6 cut(s)	179, 225, 243, 518, 749, 792
Bst4CI	ACNGT	3 cut(s)	337, 423, 445
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	1 cut(s)	653
BstAUI	TGTACA	1 cut(s)	572
BstBAI	YACGTR	1 cut(s)	57
BstC8I	GCNNGC	1 cut(s)	109
BstDEI	CTNAG	2 cut(s)	585, 830
BstDSI	CCRYGG	1 cut(s)	123
BstF5I	GGATG	4 cut(s)	124, 133, 174, 479
BstFNI	CGCG	2 cut(s)	262, 913
BstH2I	RGCGCY	1 cut(s)	86
BstHHI	GCGC	4 cut(s)	54, 85, 113, 915
BstKTI	GATC	6 cut(s)	19, 218, 259, 637, 742, 895
BstMBI	GATC	6 cut(s)	16, 215, 256, 634, 739, 892
BstMCI	CGRYCG	2 cut(s)	440, 671
BstMWI	GCNNNNNNNGC	7 cut(s)	89, 119, 268, 601, 653, 676, 710
BstNI	CCWGG	6 cut(s)	179, 225, 243, 518, 749, 792
BstNSI	RCATGY	1 cut(s)	785
BstSCI	CCNGG	7 cut(s)	177, 223, 241, 516, 747, 790, 867
BstSFI	CTRYAG	2 cut(s)	654, 917
BstSLI	GKGCMC	1 cut(s)	692
BstUI	CGCG	2 cut(s)	262, 913
BstV1I	GCAGC	3 cut(s)	258, 383, 640
BstV2I	GAAGAC	1 cut(s)	230
BstX2I	RGATCY	4 cut(s)	215, 634, 739, 892
BstYI	RGATCY	4 cut(s)	215, 634, 739, 892
BstZI	CGGCCG	1 cut(s)	668
Bsu36I	CCTNAGG	1 cut(s)	830
BsuRI	GGCC	4 cut(s)	165, 670, 768, 828
BtgI	CCRYGG	1 cut(s)	123
BtsCI	GGATG	4 cut(s)	124, 133, 174, 479
BtsI	GCAGTG	2 cut(s)	305, 564
BtsIMutI	CAGTG	3 cut(s)	305, 564, 807
Cac8I	GCNNGC	1 cut(s)	109
CfoI	GCGC	4 cut(s)	54, 85, 113, 915
Cfr10I	RCCGGY	1 cut(s)	768
Cfr13I	GGNCC	1 cut(s)	163
CsiI	ACCWGGT	2 cut(s)	516, 747
Csp6I	GTAC	3 cut(s)	58, 419, 573
CviAII	CATG	7 cut(s)	155, 275, 356, 430, 680, 782, 899
CviQI	GTAC	3 cut(s)	58, 419, 573
DdeI	CTNAG	2 cut(s)	585, 830
DpnI	GATC	6 cut(s)	18, 217, 258, 636, 741, 894
DpnII	GATC	6 cut(s)	16, 215, 256, 634, 739, 892
DraI	TTTAAA	1 cut(s)	327
EaeI	YGGCCR	1 cut(s)	668
EagI	CGGCCG	1 cut(s)	668
EclXI	CGGCCG	1 cut(s)	668
Eco147I	AGGCCT	1 cut(s)	828
Eco47III	AGCGCT	1 cut(s)	84
Eco52I	CGGCCG	1 cut(s)	668
Eco81I	CCTNAGG	1 cut(s)	830
Eco88I	CYCGRG	3 cut(s)	47, 114, 380
EcoRII	CCWGG	6 cut(s)	177, 223, 241, 516, 747, 790
EcoT22I	ATGCAT	1 cut(s)	728
FaeI	CATG	7 cut(s)	158, 278, 359, 433, 683, 785, 902
FatI	CATG	7 cut(s)	154, 274, 355, 429, 679, 781, 898
FauI	CCCGC	1 cut(s)	28
FblI	GTMKAC	3 cut(s)	132, 440, 786
Fnu4HI	GCNGC	3 cut(s)	272, 372, 654
FokI	GGATG	4 cut(s)	131, 140, 161, 466
Fsp4HI	GCNGC	3 cut(s)	272, 372, 654
GlaI	GCGC	4 cut(s)	53, 84, 112, 914
GluI	GCNGC	3 cut(s)	272, 372, 654
GsaI	CCCAGC	2 cut(s)	215, 487
GsuI	CTGGAG	1 cut(s)	630
HaeII	RGCGCY	1 cut(s)	86
HaeIII	GGCC	4 cut(s)	165, 670, 768, 828
HapII	CCGG	2 cut(s)	769, 868
HhaI	GCGC	4 cut(s)	54, 85, 113, 915
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	7 cut(s)	158, 278, 359, 433, 683, 785, 902
Hin6I	GCGC	4 cut(s)	52, 83, 111, 913
HinP1I	GCGC	4 cut(s)	52, 83, 111, 913
HincII	GTYRAC	3 cut(s)	133, 441, 548
HindII	GTYRAC	3 cut(s)	133, 441, 548
HinfI	GANTC	6 cut(s)	32, 264, 340, 351, 410, 433
HpaII	CCGG	2 cut(s)	769, 868
HphI	GGTGA	3 cut(s)	806, 847, 865
Hpy188I	TCNGA	4 cut(s)	10, 70, 104, 634
Hpy188III	TCNNGA	4 cut(s)	116, 380, 382, 609
HpyAV	CCTTC	2 cut(s)	321, 818
HpyCH4III	ACNGT	3 cut(s)	337, 423, 445
HpyCH4IV	ACGT	1 cut(s)	56
HpyF10VI	GCNNNNNNNGC	7 cut(s)	89, 119, 268, 601, 653, 676, 710
HpyF3I	CTNAG	2 cut(s)	585, 830
HpySE526I	ACGT	1 cut(s)	56
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	7 cut(s)	158, 278, 359, 433, 683, 785, 902
HspAI	GCGC	4 cut(s)	52, 83, 111, 913
Kzo9I	GATC	6 cut(s)	16, 215, 256, 634, 739, 892
LmnI	GCTCC	1 cut(s)	104
Lsp1109I	GCAGC	3 cut(s)	258, 383, 640
LweI	GCATC	3 cut(s)	108, 109, 533
MabI	ACCWGGT	2 cut(s)	516, 747
MaeII	ACGT	1 cut(s)	56
MaeIII	GTNAC	4 cut(s)	337, 398, 529, 777
MalI	GATC	6 cut(s)	18, 217, 258, 636, 741, 894
MbiI	CCGCTC	1 cut(s)	23
MboI	GATC	6 cut(s)	16, 215, 256, 634, 739, 892
MboII	GAAGA	4 cut(s)	230, 578, 749, 900
MfeI	CAATTG	1 cut(s)	714
MflI	RGATCY	4 cut(s)	215, 634, 739, 892
MhlI	GDGCHC	2 cut(s)	692, 879
MluCI	AATT	3 cut(s)	41, 169, 714
MlyI	GAGTC	3 cut(s)	258, 334, 345
MnlI	CCTC	2 cut(s)	449, 633
Mph1103I	ATGCAT	1 cut(s)	728
MseI	TTAA	1 cut(s)	326
MslI	CAYNNNNRTG	1 cut(s)	812
MspA1I	CMGCKG	2 cut(s)	139, 487
MspI	CCGG	2 cut(s)	769, 868
MspR9I	CCNGG	7 cut(s)	179, 225, 243, 518, 749, 792, 869
MunI	CAATTG	1 cut(s)	714
Mva1269I	GAATGC	1 cut(s)	628
MvaI	CCWGG	6 cut(s)	179, 225, 243, 518, 749, 792
MvnI	CGCG	2 cut(s)	262, 913
MwoI	GCNNNNNNNGC	7 cut(s)	89, 119, 268, 601, 653, 676, 710
NciI	CCSGG	1 cut(s)	869
NdeII	GATC	6 cut(s)	16, 215, 256, 634, 739, 892
NlaIII	CATG	7 cut(s)	158, 278, 359, 433, 683, 785, 902
NlaIV	GGNNCC	1 cut(s)	773
NmuCI	GTSAC	2 cut(s)	337, 529
NsiI	ATGCAT	1 cut(s)	728
NspI	RCATGY	1 cut(s)	785
PaeR7I	CTCGAG	1 cut(s)	380
PceI	AGGCCT	1 cut(s)	828
PciI	ACATGT	1 cut(s)	781
PctI	GAATGC	1 cut(s)	628
PfeI	GAWTC	3 cut(s)	32, 410, 433
PkrI	GCNGC	3 cut(s)	273, 373, 655
PleI	GAGTC	3 cut(s)	258, 334, 345
PpsI	GAGTC	3 cut(s)	258, 334, 345
Ppu21I	YACGTR	1 cut(s)	57
PscI	ACATGT	1 cut(s)	781
Psp6I	CCWGG	6 cut(s)	177, 223, 241, 516, 747, 790
PspFI	CCCAGC	2 cut(s)	211, 483
PspGI	CCWGG	6 cut(s)	177, 223, 241, 516, 747, 790
PspN4I	GGNNCC	1 cut(s)	773
PspPI	GGNCC	1 cut(s)	163
PstI	CTGCAG	1 cut(s)	658
PsuI	RGATCY	4 cut(s)	215, 634, 739, 892
PvuII	CAGCTG	2 cut(s)	139, 487
RsaI	GTAC	3 cut(s)	59, 420, 574
RsaNI	GTAC	3 cut(s)	58, 419, 573
RseI	CAYNNNNRTG	1 cut(s)	812
SalI	GTCGAC	2 cut(s)	131, 439
SaqAI	TTAA	1 cut(s)	326
SatI	GCNGC	3 cut(s)	272, 372, 654
Sau3AI	GATC	6 cut(s)	16, 215, 256, 634, 739, 892
Sau96I	GGNCC	1 cut(s)	163
SchI	GAGTC	3 cut(s)	258, 334, 345
ScrFI	CCNGG	7 cut(s)	179, 225, 243, 518, 749, 792, 869
SduI	GDGCHC	2 cut(s)	692, 879
SexAI	ACCWGGT	2 cut(s)	516, 747
SfaNI	GCATC	3 cut(s)	108, 109, 533
SfcI	CTRYAG	2 cut(s)	654, 917
Sfr274I	CTCGAG	1 cut(s)	380
SlaI	CTCGAG	1 cut(s)	380
SmiMI	CAYNNNNRTG	1 cut(s)	812
SmlI	CTYRAG	1 cut(s)	380
SmoI	CTYRAG	1 cut(s)	380
Sse9I	AATT	3 cut(s)	41, 169, 714
SseBI	AGGCCT	1 cut(s)	828
SsiI	CCGC	5 cut(s)	21, 260, 704, 732, 872
StuI	AGGCCT	1 cut(s)	828
StyD4I	CCNGG	7 cut(s)	177, 223, 241, 516, 747, 790, 867
TaaI	ACNGT	3 cut(s)	337, 423, 445
TaiI	ACGT	1 cut(s)	59
TaqI	TCGA	6 cut(s)	15, 30, 132, 381, 440, 822
TaqII	GACCGA	1 cut(s)	426
TasI	AATT	3 cut(s)	41, 169, 714
TatI	WGTACW	2 cut(s)	418, 572
TfiI	GAWTC	3 cut(s)	32, 410, 433
Tru1I	TTAA	1 cut(s)	326
Tru9I	TTAA	1 cut(s)	326
TscAI	CASTG	3 cut(s)	312, 564, 814
TseFI	GTSAC	2 cut(s)	337, 529
TseI	GCWGC	3 cut(s)	271, 371, 653
Tsp45I	GTSAC	2 cut(s)	337, 529
TspDTI	ATGAA	4 cut(s)	163, 171, 446, 579
TspRI	CASTG	3 cut(s)	312, 564, 814
VneI	GTGCAC	1 cut(s)	688
XapI	RAATTY	2 cut(s)	41, 169
XceI	RCATGY	1 cut(s)	785
XhoI	CTCGAG	1 cut(s)	380
XmiI	GTMKAC	3 cut(s)	132, 440, 786
Zsp2I	ATGCAT	1 cut(s)	728

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.