Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008662

Physical Location & Seq

Genomic Coordinates Forward (+)

1722321 .. 1729274

Gene Structure 6954 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1023900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGGCCACTACCAAGCTTGTTTTATTAACATTCGCAATCTGTCGACTTATTGTCACGGTTGGTTTAACCGTGGACCCAACGGAGCTTCTCCGCCTCGTCGTCGAAGGACAGCTGAGCTTAGACCCGTCCGACGTAAATACCGCTTCGAAAGATTTCGGCCTGATGAGCAGAGCGGAGCCCTTGGCAGTTTTACACCCAGCCTCCGCCCAAGACGTGGTGGGACTGGTGCGGACCGCATACTCTTCACCGCACGGGTTCACGGTCTCGGCCAGAGGCCACGGGCACTCCATCAACGGGCAGGCGCAGACCAACAACGGTGTCGTGATTCAGATGAGCGGGGGATCGTCAAGATCGGGGACGCCGGGTCAGGCGGCTCGAGTGTCGGAGAAGGGTATGTATGTGGATGCGTGGGGTGGGGAGTTGTGGATAGATGTGCTCAGGAGCACGCTTGAGTATGGACTCGCACCGAAGTCGTGGACTGATTATCTGTACTTATCAGTCGGCGGAACCCTTTCCAACGCCGGAATCAGTGGCCAAGCTTTCAATTACGGACCTCAGATTAGTAATGTTCATGAGCTCGACGTTGTTACAGGCAAAGGCGAGCTATTGACATGTTCAGAAGAGCAAAACTCAGAGCTGTTCCACGCTGTTCTTGGTGGGCTAGGCCAATTTGGAATCATCACTAGGGCTAGAATTGCTCTTGAACTCTCCCCCCAAAGGGTGAGGTGGATCCGAGTACTGTATTCCAATTTTTCGGCTTTTACCAAAGACCAGGAATTCCTCATCTCTCTGCATGGGCAACCCACCAGCCAGAAATTCGACTATGTCGAGGGTTTCGTTATTGTCGACGAAGGCCTCATCAACAACTGGAGGTCATCCTTCTTCTCCCCGAGCAACCCTGTCAAGATCACCTCCATTAATTCTGAGGGTGGTGTTTTATATTGCTTGGAGATTACGAAAAACTACGACGAATCGACCGCCGTTACAATTGACCAGGAAATAGAGGATTTGTTGGAAAAACTGGATTTTGTACCGACGTCAGTCTTCACGACTGATCTTCCATACGTGGATTTTCTGGACCGGGTTCACAAGGCCGAGTTGAAGCTCCGATCCAAGGGTTTGTGGGACGTCCCCCATCCATGGCTTAACCTTTTTGTCCCCAAATCAAGGATTTCTGACTTCGACGAAGGGGTGTTCAAGGGCATTCTGGGAAATAAGACAAGCGGACCCATTCTGATTTACCCCATGAACAAAGACAAGTGGGACCAGAGTAGCTCGGTGGTGACGCCCGATGAGGACGTTTTTTACCTTGTAGCGCTGCTAAGATCGGCCTTGGATACCGGGGATGAGACCCGCACCTTGGAGCACTTGACCAATCAGAACCGTCAGATCCTCAGATTCTGCGATGATGCCGGGATCGAGGTGAAACAGTACCTCCCGCACTACACTACTCGGGAGGAGTGGATGGACCACTTTGGAGATAAGTGGGACCGGTTTTACCAGAGGAAGATGAAGTTCGACCCCCGGCGCATTTTAGCCACCGGCCAACGTATTTTTAGCCCTTCATCGGGTTCTTCTGGTGGTGGGGTTGGTAGTGCTAAATTGTCAGTTTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009308 GO:0009690 GO:0009823 GO:0009987 GO:0010817 GO:0016491 GO:0016645 GO:0019139 GO:0034754 GO:0042445 GO:0042447 GO:0044237 GO:0046483 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00908 map00908

Pfam Domains

Protein Families

Cytokin-bind FAD_binding_4

Protein Analysis

539

Amino Acids

59.58

Weight (kDa)

5.46

Isoelectric Point (pI)

39.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	60 - 206	4.4e-19	FAD binding domain
Cytokin-bind	PF09265	238 - 519	1.9e-128	Cytokinin dehydrogenase 1, FAD and cytokinin binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008662)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75450 AT1G75450
fragaria_vesca	FvH4_2g39230 FvH4_2g39230 FvH4_2g39230
malus_domestica	MD08G1023900.v1.1 MD15G1021500.v1.1
prunus_persica	Prupe.1G373300_v2.0.a1
pyrus_communis	pycom08g01870 pycom15g01780
rosa_chinensis	RchiOBHm_Chr6g0301781
rosa_laevigata	RLG00000011222
rosa_multiflora	Rmu_sc0005454.1_g000020
rosa_roxburghii	Rroxscaffold_7G00166410
rosa_rugosa	Rorug06G0309500 Rorug06G0309600 Rorug06G0309700 Rorug06G0309800
rosa_samantha	Rh6AG421500 Rh6BG428300 Rh6CG434800 Rh6DG421700
rosa_wichuraiana	Rw6G036500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	1040, 1131
AccB7I	CCANNNNNTGG	1 cut(s)	214
AccBSI	CCGCTC	2 cut(s)	173, 336
AccI	GTMKAC	2 cut(s)	43, 846
AclWI	GGATC	6 cut(s)	349, 724, 737, 1104, 1384, 1424
AcoI	YGGCCR	4 cut(s)	3, 267, 532, 1543
AcsI	RAATTY	2 cut(s)	776, 815
AcyI	GRCGYC	4 cut(s)	359, 1037, 1128, 1286
AfaI	GTAC	4 cut(s)	491, 738, 1032, 1433
AfeI	AGCGCT	1 cut(s)	1317
AflIII	ACRYGT	1 cut(s)	611
AgeI	ACCGGT	1 cut(s)	1491
AgsI	TTSAA	4 cut(s)	544, 704, 1102, 1198
AhdI	GACNNNNNGTC	2 cut(s)	50, 363
AjiI	CACGTC	1 cut(s)	214
AjnI	CCWGG	2 cut(s)	771, 993
Alw21I	GWGCWC	4 cut(s)	438, 446, 579, 1368
Alw26I	GTCTC	2 cut(s)	268, 1343
AlwI	GGATC	6 cut(s)	349, 724, 737, 1104, 1384, 1424
AlwNI	CAGNNNCTG	1 cut(s)	1401
Ama87I	CYCGRG	3 cut(s)	375, 889, 1452
Aor51HI	AGCGCT	1 cut(s)	1317
ApeKI	GCWGC	1 cut(s)	1318
ApoI	RAATTY	2 cut(s)	776, 815
AseI	ATTAAT	1 cut(s)	918
AsiGI	ACCGGT	1 cut(s)	1491
Asp700I	GAANNNNTTC	2 cut(s)	152, 511
AspLEI	GCGC	3 cut(s)	304, 1318, 1530
AspS9I	GGNCC	8 cut(s)	73, 231, 551, 1078, 1226, 1264, 1468, 1489
AsuC2I	CCSGG	5 cut(s)	363, 1082, 1342, 1414, 1525
AsuHPI	GGTGA	5 cut(s)	237, 733, 901, 1294, 1435
AsuII	TTCGAA	1 cut(s)	146
AvaI	CYCGRG	3 cut(s)	375, 889, 1452
AvaII	GGWCC	8 cut(s)	73, 231, 551, 1078, 1226, 1264, 1468, 1489
BaeGI	GKGCMC	1 cut(s)	285
BalI	TGGCCA	2 cut(s)	5, 534
BamHI	GGATCC	1 cut(s)	729
BanII	GRGCYC	2 cut(s)	180, 579
BbsI	GAAGAC	1 cut(s)	1036
Bbv12I	GWGCWC	4 cut(s)	438, 446, 579, 1368
BbvI	GCAGC	1 cut(s)	1305
BccI	CCATC	3 cut(s)	296, 1143, 1459
BceAI	ACGGC	1 cut(s)	965
BcgI	CGANNNNNNTGC	2 cut(s)	453, 487
BciT130I	CCWGG	2 cut(s)	773, 995
BciVI	GTATCC	1 cut(s)	1330
BcnI	CCSGG	5 cut(s)	363, 1082, 1342, 1414, 1525
BcoDI	GTCTC	2 cut(s)	268, 1343
BfaI	CTAG	3 cut(s)	662, 684, 690
BfoI	RGCGCY	1 cut(s)	1319
BfuI	GTATCC	1 cut(s)	1330
BisI	GCNGC	2 cut(s)	372, 1319
BlpI	GCTNAGC	1 cut(s)	113
BlsI	GCNGC	2 cut(s)	373, 1320
BmcAI	AGTACT	1 cut(s)	738
Bme1390I	CCNGG	7 cut(s)	363, 773, 995, 1082, 1342, 1414, 1525
Bme18I	GGWCC	8 cut(s)	73, 231, 551, 1078, 1226, 1264, 1468, 1489
BmeRI	GACNNNNNGTC	2 cut(s)	50, 363
BmeT110I	CYCGRG	3 cut(s)	375, 889, 1452
BmgBI	CACGTC	1 cut(s)	214
BmgT120I	GGNCC	8 cut(s)	73, 231, 551, 1078, 1226, 1264, 1468, 1489
BmiI	GGNNCC	7 cut(s)	75, 177, 508, 731, 1228, 1265, 1490
BmrFI	CCNGG	7 cut(s)	363, 773, 995, 1082, 1342, 1414, 1525
BmsI	GCATC	2 cut(s)	394, 1399
BoxI	GACNNNNGTC	1 cut(s)	1040
BpiI	GAAGAC	1 cut(s)	1036
BplI	GAGNNNNNCTC	2 cut(s)	614, 646
BpmI	CTGGAG	1 cut(s)	889
Bpu10I	CCTNAGC	1 cut(s)	437
Bpu1102I	GCTNAGC	1 cut(s)	113
Bpu14I	TTCGAA	1 cut(s)	146
BpuEI	CTTGAG	1 cut(s)	470
BpuMI	CCSGG	5 cut(s)	363, 1082, 1342, 1414, 1525
BsaAI	YACGTR	1 cut(s)	1066
BsaHI	GRCGYC	4 cut(s)	359, 1037, 1128, 1286
BsaI	GGTCTC	2 cut(s)	268, 1343
BsaJI	CCNNGG	9 cut(s)	69, 180, 277, 1113, 1139, 1332, 1341, 1359, 1523
BsaWI	WCCGGW	1 cut(s)	1491
BsaXI	ACNNNNNCTCC	6 cut(s)	185, 215, 269, 299, 1419, 1449
Bse118I	RCCGGY	2 cut(s)	1491, 1541
Bse1I	ACTGG	3 cut(s)	228, 872, 1026
BseBI	CCWGG	2 cut(s)	773, 995
BseDI	CCNNGG	9 cut(s)	69, 180, 277, 1113, 1139, 1332, 1341, 1359, 1523
BseGI	GGATG	5 cut(s)	409, 875, 1135, 1351, 1470
BseMII	CTCAG	6 cut(s)	104, 451, 569, 645, 915, 1408
BseNI	ACTGG	3 cut(s)	228, 872, 1026
BseRI	GAGGAG	1 cut(s)	1472
BseSI	GKGCMC	1 cut(s)	285
BseXI	GCAGC	1 cut(s)	1305
BseYI	CCCAGC	1 cut(s)	196
Bsh1285I	CGRYCG	1 cut(s)	978
BshTI	ACCGGT	1 cut(s)	1491
BsiEI	CGRYCG	1 cut(s)	978
BsiHKAI	GWGCWC	4 cut(s)	438, 446, 579, 1368
BsiHKCI	CYCGRG	3 cut(s)	375, 889, 1452
BsiSI	CCGG	8 cut(s)	362, 522, 1081, 1341, 1413, 1492, 1525, 1542
BslFI	GGGAC	7 cut(s)	234, 370, 1115, 1139, 1142, 1277, 1502
BsmAI	GTCTC	2 cut(s)	268, 1343
BsmFI	GGGAC	7 cut(s)	234, 370, 1115, 1139, 1142, 1277, 1502
BsmI	GAATGC	1 cut(s)	1203
Bso31I	GGTCTC	2 cut(s)	268, 1343
BsoBI	CYCGRG	3 cut(s)	375, 889, 1452
Bsp119I	TTCGAA	1 cut(s)	146
Bsp1286I	GDGCHC	6 cut(s)	180, 285, 438, 446, 579, 1368
Bsp143I	GATC	9 cut(s)	341, 350, 729, 906, 1054, 1109, 1325, 1389, 1416
Bsp1720I	GCTNAGC	1 cut(s)	113
Bsp19I	CCATGG	1 cut(s)	1139
BspCNI	CTCAG	6 cut(s)	105, 450, 568, 644, 916, 1407
BspHI	TCATGA	1 cut(s)	571
BspLI	GGNNCC	7 cut(s)	75, 177, 508, 731, 1228, 1265, 1490
BspPI	GGATC	6 cut(s)	349, 724, 737, 1104, 1384, 1424
BspQI	GCTCTTC	1 cut(s)	615
BspT104I	TTCGAA	1 cut(s)	146
BspTNI	GGTCTC	2 cut(s)	268, 1343
BsrBI	CCGCTC	2 cut(s)	173, 336
BsrFI	RCCGGY	2 cut(s)	1491, 1541
BsrI	ACTGG	3 cut(s)	228, 872, 1026
BssAI	RCCGGY	2 cut(s)	1491, 1541
BssECI	CCNNGG	9 cut(s)	69, 180, 277, 1113, 1139, 1332, 1341, 1359, 1523
BssMI	GATC	9 cut(s)	341, 350, 729, 906, 1054, 1109, 1325, 1389, 1416
BssNI	GRCGYC	4 cut(s)	359, 1037, 1128, 1286
BssT1I	CCWWGG	5 cut(s)	180, 1113, 1139, 1332, 1359
Bst2UI	CCWGG	2 cut(s)	773, 995
Bst4CI	ACNGT	7 cut(s)	58, 70, 262, 317, 741, 1385, 1431
Bst6I	CTCTTC	2 cut(s)	247, 615
BstACI	GRCGYC	4 cut(s)	359, 1037, 1128, 1286
BstBAI	YACGTR	1 cut(s)	1066
BstBI	TTCGAA	1 cut(s)	146
BstC8I	GCNNGC	3 cut(s)	300, 446, 602
BstDEI	CTNAG	8 cut(s)	113, 118, 437, 555, 631, 924, 1322, 1394
BstDSI	CCRYGG	3 cut(s)	69, 277, 1139
BstF5I	GGATG	5 cut(s)	409, 875, 1135, 1351, 1470
BstH2I	RGCGCY	1 cut(s)	1319
BstHHI	GCGC	3 cut(s)	304, 1318, 1530
BstKTI	GATC	9 cut(s)	344, 353, 732, 909, 1057, 1112, 1328, 1392, 1419
BstMAI	GTCTC	2 cut(s)	268, 1343
BstMBI	GATC	9 cut(s)	341, 350, 729, 906, 1054, 1109, 1325, 1389, 1416
BstMCI	CGRYCG	1 cut(s)	978
BstMWI	GCNNNNNNNGC	2 cut(s)	165, 695
BstNI	CCWGG	2 cut(s)	773, 995
BstNSI	RCATGY	1 cut(s)	615
BstPAI	GACNNNNGTC	1 cut(s)	1040
BstSCI	CCNGG	7 cut(s)	361, 771, 993, 1080, 1340, 1412, 1523
BstSLI	GKGCMC	1 cut(s)	285
BstV1I	GCAGC	1 cut(s)	1305
BstV2I	GAAGAC	1 cut(s)	1036
BstX2I	RGATCY	2 cut(s)	729, 1389
BstYI	RGATCY	2 cut(s)	729, 1389
BsuI	GTATCC	1 cut(s)	1330
BtgI	CCRYGG	3 cut(s)	69, 277, 1139
BtgZI	GCGATG	1 cut(s)	1419
BtrI	CACGTC	1 cut(s)	214
BtsCI	GGATG	5 cut(s)	409, 875, 1135, 1351, 1470
BtsIMutI	CAGTG	1 cut(s)	535
Cac8I	GCNNGC	3 cut(s)	300, 446, 602
CaiI	CAGNNNCTG	1 cut(s)	1401
CciI	TCATGA	1 cut(s)	571
CfoI	GCGC	3 cut(s)	304, 1318, 1530
Cfr10I	RCCGGY	2 cut(s)	1491, 1541
Cfr13I	GGNCC	8 cut(s)	73, 231, 551, 1078, 1226, 1264, 1468, 1489
CpoI	CGGWCCG	1 cut(s)	231
CseI	GACGC	2 cut(s)	367, 1294
Csp6I	GTAC	4 cut(s)	490, 737, 1031, 1432
CspAI	ACCGGT	1 cut(s)	1491
CspI	CGGWCCG	1 cut(s)	231
CviAII	CATG	5 cut(s)	572, 612, 794, 1140, 1246
CviQI	GTAC	4 cut(s)	490, 737, 1031, 1432
DdeI	CTNAG	8 cut(s)	113, 118, 437, 555, 631, 924, 1322, 1394
DpnI	GATC	9 cut(s)	343, 352, 731, 908, 1056, 1111, 1327, 1391, 1418
DpnII	GATC	9 cut(s)	341, 350, 729, 906, 1054, 1109, 1325, 1389, 1416
DriI	GACNNNNNGTC	2 cut(s)	50, 363
EaeI	YGGCCR	4 cut(s)	3, 267, 532, 1543
Eam1104I	CTCTTC	2 cut(s)	247, 615
Eam1105I	GACNNNNNGTC	2 cut(s)	50, 363
EarI	CTCTTC	2 cut(s)	247, 615
EciI	GGCGGA	3 cut(s)	80, 193, 519
Ecl136II	GAGCTC	1 cut(s)	577
Eco130I	CCWWGG	5 cut(s)	180, 1113, 1139, 1332, 1359
Eco147I	AGGCCT	1 cut(s)	855
Eco24I	GRGCYC	2 cut(s)	180, 579
Eco31I	GGTCTC	2 cut(s)	268, 1343
Eco47I	GGWCC	8 cut(s)	73, 231, 551, 1078, 1226, 1264, 1468, 1489
Eco47III	AGCGCT	1 cut(s)	1317
Eco53kI	GAGCTC	1 cut(s)	577
Eco88I	CYCGRG	3 cut(s)	375, 889, 1452
EcoICRI	GAGCTC	1 cut(s)	577
EcoRI	GAATTC	1 cut(s)	776
EcoRII	CCWGG	2 cut(s)	771, 993
EcoT14I	CCWWGG	5 cut(s)	180, 1113, 1139, 1332, 1359
EcoT38I	GRGCYC	2 cut(s)	180, 579
ErhI	CCWWGG	5 cut(s)	180, 1113, 1139, 1332, 1359
FaeI	CATG	5 cut(s)	575, 615, 797, 1143, 1249
FaqI	GGGAC	7 cut(s)	234, 370, 1115, 1139, 1142, 1277, 1502
FatI	CATG	5 cut(s)	571, 611, 793, 1139, 1245
FauI	CCCGC	3 cut(s)	329, 1361, 1446
FblI	GTMKAC	2 cut(s)	43, 846
Fnu4HI	GCNGC	2 cut(s)	372, 1319
FokI	GGATG	5 cut(s)	416, 862, 1122, 1358, 1477
FriOI	GRGCYC	2 cut(s)	180, 579
Fsp4HI	GCNGC	2 cut(s)	372, 1319
FspBI	CTAG	3 cut(s)	662, 684, 690
GlaI	GCGC	3 cut(s)	303, 1317, 1529
GluI	GCNGC	2 cut(s)	372, 1319
GsaI	CCCAGC	1 cut(s)	200
GsuI	CTGGAG	1 cut(s)	889
HaeII	RGCGCY	1 cut(s)	1319
HapII	CCGG	8 cut(s)	362, 522, 1081, 1341, 1413, 1492, 1525, 1542
HgaI	GACGC	2 cut(s)	367, 1294
HhaI	GCGC	3 cut(s)	304, 1318, 1530
Hin1I	GRCGYC	4 cut(s)	359, 1037, 1128, 1286
Hin1II	CATG	5 cut(s)	575, 615, 797, 1143, 1249
Hin6I	GCGC	3 cut(s)	302, 1316, 1528
HinP1I	GCGC	3 cut(s)	302, 1316, 1528
HincII	GTYRAC	2 cut(s)	44, 847
HindII	GTYRAC	2 cut(s)	44, 847
HindIII	AAGCTT	2 cut(s)	14, 537
HinfI	GANTC	6 cut(s)	325, 459, 525, 675, 971, 1398
HpaII	CCGG	8 cut(s)	362, 522, 1081, 1341, 1413, 1492, 1525, 1542
HphI	GGTGA	5 cut(s)	237, 733, 901, 1294, 1435
Hpy166II	GTNNAC	6 cut(s)	44, 73, 258, 477, 847, 1087
Hpy188III	TCNNGA	9 cut(s)	322, 348, 439, 572, 701, 904, 1048, 1076, 1454
Hpy8I	GTNNAC	6 cut(s)	44, 73, 258, 477, 847, 1087
Hpy99I	CGWCG	8 cut(s)	101, 104, 134, 584, 851, 971, 1039, 1187
HpyAV	CCTTC	6 cut(s)	98, 382, 845, 889, 1181, 1572
HpyCH4III	ACNGT	7 cut(s)	58, 70, 262, 317, 741, 1385, 1431
HpyCH4IV	ACGT	8 cut(s)	132, 213, 582, 1037, 1065, 1128, 1299, 1549
HpyCH4V	TGCA	1 cut(s)	793
HpyF10VI	GCNNNNNNNGC	2 cut(s)	165, 695
HpyF3I	CTNAG	8 cut(s)	113, 118, 437, 555, 631, 924, 1322, 1394
HpySE526I	ACGT	8 cut(s)	132, 213, 582, 1037, 1065, 1128, 1299, 1549
Hsp92I	GRCGYC	4 cut(s)	359, 1037, 1128, 1286
Hsp92II	CATG	5 cut(s)	575, 615, 797, 1143, 1249
HspAI	GCGC	3 cut(s)	302, 1316, 1528
Kzo9I	GATC	9 cut(s)	341, 350, 729, 906, 1054, 1109, 1325, 1389, 1416
LguI	GCTCTTC	1 cut(s)	615
LmnI	GCTCC	5 cut(s)	82, 175, 441, 1110, 1363
Lsp1109I	GCAGC	1 cut(s)	1305
LweI	GCATC	2 cut(s)	394, 1399
MaeI	CTAG	3 cut(s)	662, 684, 690
MaeII	ACGT	8 cut(s)	132, 213, 582, 1037, 1065, 1128, 1299, 1549
MaeIII	GTNAC	4 cut(s)	52, 586, 982, 1282
MalI	GATC	9 cut(s)	343, 352, 731, 908, 1056, 1111, 1327, 1391, 1418
MbiI	CCGCTC	2 cut(s)	173, 336
MboI	GATC	9 cut(s)	341, 350, 729, 906, 1054, 1109, 1325, 1389, 1416
MboII	GAAGA	7 cut(s)	234, 632, 874, 1036, 1049, 1519, 1566
MfeI	CAATTG	1 cut(s)	987
MflI	RGATCY	2 cut(s)	729, 1389
MhlI	GDGCHC	6 cut(s)	180, 285, 438, 446, 579, 1368
MlsI	TGGCCA	2 cut(s)	5, 534
MluCI	AATT	9 cut(s)	544, 668, 693, 748, 776, 815, 919, 987, 1601
MluNI	TGGCCA	2 cut(s)	5, 534
MlyI	GAGTC	1 cut(s)	453
MmeI	TCCRAC	4 cut(s)	153, 363, 540, 993
Mox20I	TGGCCA	2 cut(s)	5, 534
MroXI	GAANNNNTTC	2 cut(s)	152, 511
MscI	TGGCCA	2 cut(s)	5, 534
MseI	TTAA	4 cut(s)	26, 65, 918, 1146
Msp20I	TGGCCA	2 cut(s)	5, 534
MspA1I	CMGCKG	1 cut(s)	112
MspI	CCGG	8 cut(s)	362, 522, 1081, 1341, 1413, 1492, 1525, 1542
MspR9I	CCNGG	7 cut(s)	363, 773, 995, 1082, 1342, 1414, 1525
MunI	CAATTG	1 cut(s)	987
Mva1269I	GAATGC	1 cut(s)	1203
MvaI	CCWGG	2 cut(s)	773, 995
MwoI	GCNNNNNNNGC	2 cut(s)	165, 695
NciI	CCSGG	5 cut(s)	363, 1082, 1342, 1414, 1525
NcoI	CCATGG	1 cut(s)	1139
NdeII	GATC	9 cut(s)	341, 350, 729, 906, 1054, 1109, 1325, 1389, 1416
NlaIII	CATG	5 cut(s)	575, 615, 797, 1143, 1249
NlaIV	GGNNCC	7 cut(s)	75, 177, 508, 731, 1228, 1265, 1490
NmeAIII	GCCGAG	2 cut(s)	245, 1120
NmuCI	GTSAC	2 cut(s)	52, 1282
NspI	RCATGY	1 cut(s)	615
NspV	TTCGAA	1 cut(s)	146
PaeR7I	CTCGAG	1 cut(s)	375
PagI	TCATGA	1 cut(s)	571
PceI	AGGCCT	1 cut(s)	855
PciI	ACATGT	1 cut(s)	611
PciSI	GCTCTTC	1 cut(s)	615
PcsI	WCGNNNNNNNCGW	3 cut(s)	825, 834, 843
PctI	GAATGC	1 cut(s)	1203
PdmI	GAANNNNTTC	2 cut(s)	152, 511
PfeI	GAWTC	5 cut(s)	325, 525, 675, 971, 1398
PflFI	GACNNNGTC	1 cut(s)	824
PflMI	CCANNNNNTGG	1 cut(s)	214
PinAI	ACCGGT	1 cut(s)	1491
PkrI	GCNGC	2 cut(s)	373, 1320
PleI	GAGTC	1 cut(s)	453
PpsI	GAGTC	1 cut(s)	453
Ppu21I	YACGTR	1 cut(s)	1066
PscI	ACATGT	1 cut(s)	611
PshAI	GACNNNNGTC	1 cut(s)	1040
PshBI	ATTAAT	1 cut(s)	918
Psp124BI	GAGCTC	1 cut(s)	579
Psp6I	CCWGG	2 cut(s)	771, 993
PspFI	CCCAGC	1 cut(s)	196
PspGI	CCWGG	2 cut(s)	771, 993
PspN4I	GGNNCC	7 cut(s)	75, 177, 508, 731, 1228, 1265, 1490
PspPI	GGNCC	8 cut(s)	73, 231, 551, 1078, 1226, 1264, 1468, 1489
PspXI	VCTCGAGB	1 cut(s)	375
PstNI	CAGNNNCTG	1 cut(s)	1401
PsuI	RGATCY	2 cut(s)	729, 1389
PsyI	GACNNNGTC	1 cut(s)	824
PvuII	CAGCTG	1 cut(s)	112
RsaI	GTAC	4 cut(s)	491, 738, 1032, 1433
RsaNI	GTAC	4 cut(s)	490, 737, 1031, 1432
Rsr2I	CGGWCCG	1 cut(s)	231
RsrII	CGGWCCG	1 cut(s)	231
SacI	GAGCTC	1 cut(s)	579
SalI	GTCGAC	2 cut(s)	42, 845
SapI	GCTCTTC	1 cut(s)	615
SaqAI	TTAA	4 cut(s)	26, 65, 918, 1146
SatI	GCNGC	2 cut(s)	372, 1319
Sau3AI	GATC	9 cut(s)	341, 350, 729, 906, 1054, 1109, 1325, 1389, 1416
Sau96I	GGNCC	8 cut(s)	73, 231, 551, 1078, 1226, 1264, 1468, 1489
ScaI	AGTACT	1 cut(s)	738
SchI	GAGTC	1 cut(s)	453
ScrFI	CCNGG	7 cut(s)	363, 773, 995, 1082, 1342, 1414, 1525
SduI	GDGCHC	6 cut(s)	180, 285, 438, 446, 579, 1368
SfaNI	GCATC	2 cut(s)	394, 1399
Sfr274I	CTCGAG	1 cut(s)	375
SfuI	TTCGAA	1 cut(s)	146
SinI	GGWCC	8 cut(s)	73, 231, 551, 1078, 1226, 1264, 1468, 1489
SlaI	CTCGAG	1 cut(s)	375
SmlI	CTYRAG	2 cut(s)	375, 449
SmoI	CTYRAG	2 cut(s)	375, 449
Sse9I	AATT	9 cut(s)	544, 668, 693, 748, 776, 815, 919, 987, 1601
SseBI	AGGCCT	1 cut(s)	855
SspMI	CTAG	3 cut(s)	662, 684, 690
SstI	GAGCTC	1 cut(s)	579
StuI	AGGCCT	1 cut(s)	855
StyD4I	CCNGG	7 cut(s)	361, 771, 993, 1080, 1340, 1412, 1523
StyI	CCWWGG	5 cut(s)	180, 1113, 1139, 1332, 1359
TaaI	ACNGT	7 cut(s)	58, 70, 262, 317, 741, 1385, 1431
TaiI	ACGT	8 cut(s)	135, 216, 585, 1040, 1068, 1131, 1302, 1552
TasI	AATT	9 cut(s)	544, 668, 693, 748, 776, 815, 919, 987, 1601
TatI	WGTACW	2 cut(s)	489, 736
TauI	GCSGC	1 cut(s)	374
TfiI	GAWTC	5 cut(s)	325, 525, 675, 971, 1398
Tru1I	TTAA	4 cut(s)	26, 65, 918, 1146
Tru9I	TTAA	4 cut(s)	26, 65, 918, 1146
TscAI	CASTG	1 cut(s)	535
TseFI	GTSAC	2 cut(s)	52, 1282
TseI	GCWGC	1 cut(s)	1318
Tsp45I	GTSAC	2 cut(s)	52, 1282
TspDTI	ATGAA	4 cut(s)	560, 1262, 1526, 1554
TspGWI	ACGGA	2 cut(s)	95, 564
TspRI	CASTG	1 cut(s)	535
Tth111I	GACNNNGTC	1 cut(s)	824
Van91I	CCANNNNNTGG	1 cut(s)	214
VpaK11BI	GGWCC	8 cut(s)	73, 231, 551, 1078, 1226, 1264, 1468, 1489
VspI	ATTAAT	1 cut(s)	918
XapI	RAATTY	2 cut(s)	776, 815
XceI	RCATGY	1 cut(s)	615
XcmI	CCANNNNNNNNNTGG	1 cut(s)	650
XhoI	CTCGAG	1 cut(s)	375
XmiI	GTMKAC	2 cut(s)	43, 846
XmnI	GAANNNNTTC	2 cut(s)	152, 511
XspI	CTAG	3 cut(s)	662, 684, 690
ZraI	GACGTC	2 cut(s)	1038, 1129
ZrmI	AGTACT	1 cut(s)	738

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.