Rw6G036500

Cytokinin dehydrogenase

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
Physical Location & Seq
Reverse (-)
60940398 .. 60945080
4683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G036500.1

Sequence Viewer

Length: 1287 bp
ATGGCTACAAAGCTTGTTTTGATTACATTCGCGATATGTAGGCTGCTTATTACGGTGGGGCTGACCATGGACCCGGCGGAGCTGCTCCGTCTCGTGGTGGACGGACAGCTCAGCCTGGATCCGTCCGACGTGGAGACTGCGTCCAAGGACTTTGGTCTCATGAGCCGAGCGGAGCCGTTGGCCGTTATGCACCCGGCTTCGGCGGAAGATGTAACGCGGCTCGTGCGGGCTGCGTACTCTTCCGCCAAGGGCTTCAGCGTCTCGGCTCGTGGGCATGGCCACTCCATCAACGGCCAGGCCCAGGCGAGAAATGGGGTGGTGGTTGAAATGAGCGGAGGGAACCGGGTTGGATCGGGGAAGCCTGCCGCCGGCCGGAGTGAGACTGTGGGTCGGGTTGTTTCGGAGAAGGGTTTGTACATGGACGCGTGGGGTGGGGAGCTGTGGATAGAGGTGTTGAGGGCAACGTTGGAGTATGGACTCGCCCCCAAGTCGTGGACAGACTATTTGTACTTGTCAGTAGGCGGTACACTCTCCAACGCCGGAATCAGTGGCCAAGCTTTCAACCATGGACCTCAGATTAGTAACGTTCTTGAGGTTGACGTTGTTACAGGCAAAGGCGAGCTCTTGACATGTTCAGAAGAGAGAAACTCAGAGCTGTTCCACGCTGTTCTTGGTGGGCTTGGCCAGTTTGGAATCATCACTAGGGCTAGAATTGCCCTTGAACCTGCCCCCAAAAGGGTGAGGTGGATCCGAGTACTGTATTCAACTTTTTCGGCCTTTACCAAAGACCAGGAATATCTGATCTCTCTTCATGGGCAACCCAGCAGCCAGAAATTCGATTACGTTGAGGGTTTCGTTATTGTCGACGAAGGCCTCATCAACAACTGGAGGTCATCTTTCTTCTCCCCAAGCAACCCTGTCAAGATCTCCTCCATTGATTTTAATGGGAATGGAGGTGTTTTATATTGCTTGGAGATAACCAAAAACTACGATGATTCAACCGCTGATACTATTGACCAGGAAATAGAGGCTTTGTTAGAAAAACTGGATTTCGTACCAACATCAGTGTTCACTACTGATCTTCCCTACGTGGATTTTCTGGACCGGGTTCACAAGGCCGAGTTGAAGCTCCGGTCCAAGGGCTTGTGGGATGTTCCCCATCCTTGGCTCAACCTTTTTGTCCCCAAATCAAGGATTGCTGACTTCGACGAGGGAGTGTTCAAGGGCATTTTGGGAAACAAAACAAGCGGCCCGGTTCTCATCTACCCCATGAACAATGACAAGTAA

Protein Analysis

428

Amino Acids

46.74

Weight (kDa)

5.49

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 59 - 212 2.6e-17 FAD binding domain
Cytokin-bind PF09265 244 - 428 1.1e-77 Cytokinin dehydrogenase 1, FAD and cytokinin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 733
AccB7I CCANNNNNTGG 1 cut(s) 492
AccBSI CCGCTC 2 cut(s) 170, 333
AccI GTMKAC 1 cut(s) 864
AccII CGCG 3 cut(s) 32, 217, 425
AclI AACGTT 2 cut(s) 464, 585
AclWI GGATC 5 cut(s) 113, 126, 358, 742, 755
AcoI YGGCCR 6 cut(s) 180, 277, 292, 370, 550, 682
AcsI RAATTY 1 cut(s) 833
AcuI CTGAAG 1 cut(s) 238
AfaI GTAC 6 cut(s) 236, 416, 509, 526, 756, 1056
AflIII ACRYGT 2 cut(s) 423, 629
AgsI TTSAA 7 cut(s) 326, 562, 722, 765, 999, 1126, 1222
AhdI GACNNNNNGTC 1 cut(s) 387
AjiI CACGTC 1 cut(s) 130
AjnI CCWGG 5 cut(s) 114, 294, 300, 789, 1017
AluBI AGCT 8 cut(s) 13, 82, 109, 439, 557, 622, 655, 1129
AluI AGCT 8 cut(s) 13, 82, 109, 439, 557, 622, 655, 1129
Alw21I GWGCWC 1 cut(s) 624
Alw26I GTCTC 5 cut(s) 95, 128, 161, 265, 374
AlwI GGATC 5 cut(s) 113, 126, 358, 742, 755
ApeKI GCWGC 4 cut(s) 43, 82, 230, 825
ApoI RAATTY 1 cut(s) 833
ArsI GACNNNNNNTTYG 2 cut(s) 487, 519
AspS9I GGNCC 6 cut(s) 70, 298, 569, 1102, 1134, 1250
AsuC2I CCSGG 5 cut(s) 74, 194, 344, 1106, 1253
AsuHPI GGTGA 1 cut(s) 751
AvaII GGWCC 4 cut(s) 70, 569, 1102, 1134
BalI TGGCCA 3 cut(s) 279, 552, 684
BamHI GGATCC 2 cut(s) 118, 747
BanII GRGCYC 1 cut(s) 624
BarI GAAGNNNNNNTAC 2 cut(s) 398, 430
BauI CACGAG 3 cut(s) 92, 221, 267
Bbv12I GWGCWC 1 cut(s) 624
BbvI GCAGC 4 cut(s) 30, 69, 217, 837
BccI CCATC 2 cut(s) 293, 1167
BceAI ACGGC 3 cut(s) 160, 167, 307
BciT130I CCWGG 5 cut(s) 116, 296, 302, 791, 1019
BcnI CCSGG 5 cut(s) 74, 194, 344, 1106, 1253
BcoDI GTCTC 5 cut(s) 95, 128, 161, 265, 374
BfaI CTAG 2 cut(s) 702, 708
BfuAI ACCTGC 1 cut(s) 733
BglII AGATCT 1 cut(s) 924
BisI GCNGC 7 cut(s) 44, 83, 218, 231, 366, 826, 1249
BlpI GCTNAGC 1 cut(s) 110
BlsI GCNGC 7 cut(s) 45, 84, 219, 232, 367, 827, 1250
BmcAI AGTACT 1 cut(s) 756
Bme18I GGWCC 4 cut(s) 70, 569, 1102, 1134
BmeRI GACNNNNNGTC 1 cut(s) 387
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 6 cut(s) 70, 298, 569, 1102, 1134, 1250
BmiI GGNNCC 5 cut(s) 72, 120, 174, 341, 749
BoxI GACNNNNGTC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 632, 664
BpmI CTGGAG 1 cut(s) 907
Bpu1102I GCTNAGC 1 cut(s) 110
BpuEI CTTGAG 1 cut(s) 611
BpuMI CCSGG 5 cut(s) 74, 194, 344, 1106, 1253
BsaAI YACGTR 1 cut(s) 1090
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 7 cut(s) 66, 144, 246, 300, 565, 1137, 1163
BsaWI WCCGGW 1 cut(s) 1131
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bse118I RCCGGY 1 cut(s) 368
Bse1I ACTGG 3 cut(s) 685, 890, 1050
BseBI CCWGG 5 cut(s) 116, 296, 302, 791, 1019
BseDI CCNNGG 7 cut(s) 66, 144, 246, 300, 565, 1137, 1163
BseGI GGATG 2 cut(s) 1156, 1159
BseMII CTCAG 3 cut(s) 124, 587, 663
BseNI ACTGG 3 cut(s) 685, 890, 1050
BseRI GAGGAG 1 cut(s) 919
BseX3I CGGCCG 1 cut(s) 370
BseXI GCAGC 4 cut(s) 30, 69, 217, 837
BseYI CCCAGC 1 cut(s) 821
Bsh1236I CGCG 3 cut(s) 32, 217, 425
Bsh1285I CGRYCG 1 cut(s) 373
BsiEI CGRYCG 1 cut(s) 373
BsiHKAI GWGCWC 1 cut(s) 624
BsiSI CCGG 9 cut(s) 74, 194, 343, 369, 373, 540, 1105, 1132, 1253
BslFI GGGAC 1 cut(s) 1166
BsmAI GTCTC 5 cut(s) 95, 128, 161, 265, 374
BsmBI CGTCTC 2 cut(s) 95, 265
BsmFI GGGAC 1 cut(s) 1166
Bso31I GGTCTC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 624
Bsp1407I TGTACA 1 cut(s) 414
Bsp143I GATC 6 cut(s) 118, 350, 747, 801, 924, 1078
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 2 cut(s) 66, 565
Bsp68I TCGCGA 1 cut(s) 32
BspCNI CTCAG 3 cut(s) 123, 586, 662
BspFNI CGCG 3 cut(s) 32, 217, 425
BspHI TCATGA 1 cut(s) 159
BspLI GGNNCC 5 cut(s) 72, 120, 174, 341, 749
BspMI ACCTGC 1 cut(s) 733
BspPI GGATC 5 cut(s) 113, 126, 358, 742, 755
BspTNI GGTCTC 1 cut(s) 161
BsrBI CCGCTC 2 cut(s) 170, 333
BsrFI RCCGGY 1 cut(s) 368
BsrGI TGTACA 1 cut(s) 414
BsrI ACTGG 3 cut(s) 685, 890, 1050
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 7 cut(s) 66, 144, 246, 300, 565, 1137, 1163
BssMI GATC 6 cut(s) 118, 350, 747, 801, 924, 1078
BssSI CACGAG 3 cut(s) 92, 221, 267
BssT1I CCWWGG 6 cut(s) 66, 144, 246, 565, 1137, 1163
Bst2BI CACGAG 3 cut(s) 92, 221, 267
Bst2UI CCWGG 5 cut(s) 116, 296, 302, 791, 1019
Bst4CI ACNGT 3 cut(s) 55, 385, 759
Bst6I CTCTTC 3 cut(s) 244, 633, 813
BstAUI TGTACA 1 cut(s) 414
BstBAI YACGTR 1 cut(s) 1090
BstC8I GCNNGC 4 cut(s) 228, 363, 370, 620
BstDEI CTNAG 3 cut(s) 110, 573, 649
BstDSI CCRYGG 2 cut(s) 66, 565
BstF5I GGATG 2 cut(s) 1156, 1159
BstFNI CGCG 3 cut(s) 32, 217, 425
BstKTI GATC 6 cut(s) 121, 353, 750, 804, 927, 1081
BstMAI GTCTC 5 cut(s) 95, 128, 161, 265, 374
BstMBI GATC 6 cut(s) 118, 350, 747, 801, 924, 1078
BstMCI CGRYCG 1 cut(s) 373
BstMWI GCNNNNNNNGC 2 cut(s) 223, 713
BstNI CCWGG 5 cut(s) 116, 296, 302, 791, 1019
BstNSI RCATGY 1 cut(s) 633
BstPAI GACNNNNGTC 1 cut(s) 153
BstUI CGCG 3 cut(s) 32, 217, 425
BstV1I GCAGC 4 cut(s) 30, 69, 217, 837
BstX2I RGATCY 3 cut(s) 118, 747, 924
BstYI RGATCY 3 cut(s) 118, 747, 924
BstZI CGGCCG 1 cut(s) 370
BtgI CCRYGG 2 cut(s) 66, 565
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 2 cut(s) 1156, 1159
BtsIMutI CAGTG 2 cut(s) 553, 1071
BtuMI TCGCGA 1 cut(s) 32
BveI ACCTGC 1 cut(s) 733
Cac8I GCNNGC 4 cut(s) 228, 363, 370, 620
CciI TCATGA 1 cut(s) 159
Cfr10I RCCGGY 1 cut(s) 368
Cfr13I GGNCC 6 cut(s) 70, 298, 569, 1102, 1134, 1250
CseI GACGC 3 cut(s) 129, 247, 431
Csp6I GTAC 6 cut(s) 235, 415, 508, 525, 755, 1055
CviAII CATG 8 cut(s) 67, 160, 275, 418, 566, 630, 812, 1270
CviQI GTAC 6 cut(s) 235, 415, 508, 525, 755, 1055
DdeI CTNAG 3 cut(s) 110, 573, 649
DpnI GATC 6 cut(s) 120, 352, 749, 803, 926, 1080
DpnII GATC 6 cut(s) 118, 350, 747, 801, 924, 1078
DriI GACNNNNNGTC 1 cut(s) 387
EaeI YGGCCR 6 cut(s) 180, 277, 292, 370, 550, 682
EagI CGGCCG 1 cut(s) 370
Eam1104I CTCTTC 3 cut(s) 244, 633, 813
Eam1105I GACNNNNNGTC 1 cut(s) 387
EarI CTCTTC 3 cut(s) 244, 633, 813
EciI GGCGGA 3 cut(s) 92, 218, 232
Ecl136II GAGCTC 1 cut(s) 622
EclXI CGGCCG 1 cut(s) 370
Eco130I CCWWGG 6 cut(s) 66, 144, 246, 565, 1137, 1163
Eco147I AGGCCT 1 cut(s) 873
Eco24I GRGCYC 1 cut(s) 624
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 4 cut(s) 70, 569, 1102, 1134
Eco52I CGGCCG 1 cut(s) 370
Eco53kI GAGCTC 1 cut(s) 622
Eco57I CTGAAG 1 cut(s) 238
EcoICRI GAGCTC 1 cut(s) 622
EcoRII CCWGG 5 cut(s) 114, 294, 300, 789, 1017
EcoT14I CCWWGG 6 cut(s) 66, 144, 246, 565, 1137, 1163
EcoT38I GRGCYC 1 cut(s) 624
ErhI CCWWGG 6 cut(s) 66, 144, 246, 565, 1137, 1163
Esp3I CGTCTC 2 cut(s) 95, 265
FaeI CATG 8 cut(s) 70, 163, 278, 421, 569, 633, 815, 1273
FaqI GGGAC 1 cut(s) 1166
FatI CATG 8 cut(s) 66, 159, 274, 417, 565, 629, 811, 1269
FauI CCCGC 1 cut(s) 219
FblI GTMKAC 1 cut(s) 864
Fnu4HI GCNGC 7 cut(s) 44, 83, 218, 231, 366, 826, 1249
FokI GGATG 2 cut(s) 1146, 1163
FriOI GRGCYC 1 cut(s) 624
Fsp4HI GCNGC 7 cut(s) 44, 83, 218, 231, 366, 826, 1249
FspBI CTAG 2 cut(s) 702, 708
GluI GCNGC 7 cut(s) 44, 83, 218, 231, 366, 826, 1249
GsaI CCCAGC 1 cut(s) 825
GsuI CTGGAG 1 cut(s) 907
HapII CCGG 9 cut(s) 74, 194, 343, 369, 373, 540, 1105, 1132, 1253
HgaI GACGC 3 cut(s) 129, 247, 431
Hin1II CATG 8 cut(s) 70, 163, 278, 421, 569, 633, 815, 1273
HincII GTYRAC 2 cut(s) 598, 865
HindII GTYRAC 2 cut(s) 598, 865
HindIII AAGCTT 2 cut(s) 11, 555
HinfI GANTC 4 cut(s) 477, 543, 693, 995
HpaII CCGG 9 cut(s) 74, 194, 343, 369, 373, 540, 1105, 1132, 1253
HphI GGTGA 1 cut(s) 751
Hpy166II GTNNAC 7 cut(s) 100, 495, 527, 598, 865, 1071, 1111
Hpy188I TCNGA 7 cut(s) 127, 403, 576, 637, 652, 752, 801
Hpy188III TCNNGA 6 cut(s) 31, 160, 590, 625, 922, 1100
Hpy8I GTNNAC 7 cut(s) 100, 495, 527, 598, 865, 1071, 1111
Hpy99I CGWCG 3 cut(s) 131, 869, 1211
HpyAV CCTTC 2 cut(s) 400, 863
HpyCH4III ACNGT 3 cut(s) 55, 385, 759
HpyCH4IV ACGT 6 cut(s) 129, 464, 585, 600, 843, 1089
HpyCH4V TGCA 1 cut(s) 190
HpyF10VI GCNNNNNNNGC 2 cut(s) 223, 713
HpyF3I CTNAG 3 cut(s) 110, 573, 649
HpySE526I ACGT 6 cut(s) 129, 464, 585, 600, 843, 1089
Hsp92II CATG 8 cut(s) 70, 163, 278, 421, 569, 633, 815, 1273
KroI GCCGGC 1 cut(s) 368
KroNI GCCGGC 1 cut(s) 370
Kzo9I GATC 6 cut(s) 118, 350, 747, 801, 924, 1078
LmnI GCTCC 5 cut(s) 79, 90, 172, 436, 1134
Lsp1109I GCAGC 4 cut(s) 30, 69, 217, 837
MaeI CTAG 2 cut(s) 702, 708
MaeII ACGT 6 cut(s) 129, 464, 585, 600, 843, 1089
MaeIII GTNAC 3 cut(s) 211, 581, 604
MalI GATC 6 cut(s) 120, 352, 749, 803, 926, 1080
MbiI CCGCTC 2 cut(s) 170, 333
MboI GATC 6 cut(s) 118, 350, 747, 801, 924, 1078
MboII GAAGA 6 cut(s) 218, 231, 650, 800, 892, 1073
MflI RGATCY 3 cut(s) 118, 747, 924
MhlI GDGCHC 1 cut(s) 624
MlsI TGGCCA 3 cut(s) 279, 552, 684
MluCI AATT 2 cut(s) 711, 833
MluI ACGCGT 1 cut(s) 423
MluNI TGGCCA 3 cut(s) 279, 552, 684
MlyI GAGTC 1 cut(s) 471
MmeI TCCRAC 4 cut(s) 150, 328, 447, 558
Mox20I TGGCCA 3 cut(s) 279, 552, 684
MroNI GCCGGC 1 cut(s) 368
MscI TGGCCA 3 cut(s) 279, 552, 684
MseI TTAA 1 cut(s) 942
Msp20I TGGCCA 3 cut(s) 279, 552, 684
MspA1I CMGCKG 1 cut(s) 1004
MspI CCGG 9 cut(s) 74, 194, 343, 369, 373, 540, 1105, 1132, 1253
MvaI CCWGG 5 cut(s) 116, 296, 302, 791, 1019
MvnI CGCG 3 cut(s) 32, 217, 425
MwoI GCNNNNNNNGC 2 cut(s) 223, 713
NaeI GCCGGC 1 cut(s) 370
NciI CCSGG 5 cut(s) 74, 194, 344, 1106, 1253
NcoI CCATGG 2 cut(s) 66, 565
NdeII GATC 6 cut(s) 118, 350, 747, 801, 924, 1078
NgoMIV GCCGGC 1 cut(s) 368
NlaIII CATG 8 cut(s) 70, 163, 278, 421, 569, 633, 815, 1273
NlaIV GGNNCC 5 cut(s) 72, 120, 174, 341, 749
NmeAIII GCCGAG 3 cut(s) 191, 242, 1144
NruI TCGCGA 1 cut(s) 32
NspI RCATGY 1 cut(s) 633
PagI TCATGA 1 cut(s) 159
PceI AGGCCT 1 cut(s) 873
PciI ACATGT 1 cut(s) 629
PcsI WCGNNNNNNNCGW 1 cut(s) 861
PdiI GCCGGC 1 cut(s) 370
PfeI GAWTC 3 cut(s) 543, 693, 995
PflFI GACNNNGTC 1 cut(s) 139
PflMI CCANNNNNTGG 1 cut(s) 492
PkrI GCNGC 7 cut(s) 45, 84, 219, 232, 367, 827, 1250
PleI GAGTC 1 cut(s) 471
PpsI GAGTC 1 cut(s) 471
Ppu21I YACGTR 1 cut(s) 1090
PscI ACATGT 1 cut(s) 629
PshAI GACNNNNGTC 1 cut(s) 153
Psp124BI GAGCTC 1 cut(s) 624
Psp1406I AACGTT 2 cut(s) 464, 585
Psp6I CCWGG 5 cut(s) 114, 294, 300, 789, 1017
PspFI CCCAGC 1 cut(s) 821
PspGI CCWGG 5 cut(s) 114, 294, 300, 789, 1017
PspN4I GGNNCC 5 cut(s) 72, 120, 174, 341, 749
PspPI GGNCC 6 cut(s) 70, 298, 569, 1102, 1134, 1250
PsuI RGATCY 3 cut(s) 118, 747, 924
PsyI GACNNNGTC 1 cut(s) 139
RruI TCGCGA 1 cut(s) 32
RsaI GTAC 6 cut(s) 236, 416, 509, 526, 756, 1056
RsaNI GTAC 6 cut(s) 235, 415, 508, 525, 755, 1055
SacI GAGCTC 1 cut(s) 624
SalI GTCGAC 1 cut(s) 863
SaqAI TTAA 1 cut(s) 942
SatI GCNGC 7 cut(s) 44, 83, 218, 231, 366, 826, 1249
Sau3AI GATC 6 cut(s) 118, 350, 747, 801, 924, 1078
Sau96I GGNCC 6 cut(s) 70, 298, 569, 1102, 1134, 1250
ScaI AGTACT 1 cut(s) 756
SchI GAGTC 1 cut(s) 471
SduI GDGCHC 1 cut(s) 624
SinI GGWCC 4 cut(s) 70, 569, 1102, 1134
SmlI CTYRAG 1 cut(s) 590
SmoI CTYRAG 1 cut(s) 590
Sse9I AATT 2 cut(s) 711, 833
SseBI AGGCCT 1 cut(s) 873
SspMI CTAG 2 cut(s) 702, 708
SstI GAGCTC 1 cut(s) 624
StuI AGGCCT 1 cut(s) 873
StyI CCWWGG 6 cut(s) 66, 144, 246, 565, 1137, 1163
TaaI ACNGT 3 cut(s) 55, 385, 759
TaiI ACGT 6 cut(s) 132, 467, 588, 603, 846, 1092
TaqI TCGA 3 cut(s) 837, 864, 1206
TasI AATT 2 cut(s) 711, 833
TatI WGTACW 3 cut(s) 414, 507, 754
TauI GCSGC 3 cut(s) 220, 368, 1251
TfiI GAWTC 3 cut(s) 543, 693, 995
Tru1I TTAA 1 cut(s) 942
Tru9I TTAA 1 cut(s) 942
TscAI CASTG 2 cut(s) 553, 1071
TseI GCWGC 4 cut(s) 43, 82, 230, 825
TspDTI ATGAA 2 cut(s) 800, 1286
TspGWI ACGGA 3 cut(s) 77, 111, 117
TspRI CASTG 2 cut(s) 553, 1071
Tth111I GACNNNGTC 1 cut(s) 139
Van91I CCANNNNNTGG 1 cut(s) 492
VpaK11BI GGWCC 4 cut(s) 70, 569, 1102, 1134
XapI RAATTY 1 cut(s) 833
XceI RCATGY 1 cut(s) 633
XcmI CCANNNNNNNNNTGG 2 cut(s) 308, 668
XmiI GTMKAC 1 cut(s) 864
XspI CTAG 2 cut(s) 702, 708
ZrmI AGTACT 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.