Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008662

Physical Location & Seq

Genomic Coordinates Forward (+)

1244122 .. 1250499

Gene Structure 6378 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1021500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGGCAACTACCAAGCTTGTTTTAATTACATTCGCAATCTGTCGACTTATTCTCACGGTTGGTTTAACCGTGGATCCAACGGAGCTTCTCCGCCTCGTCGTCGACGGACAGCTCAGTGTAGACCCATCTGACGTGGATACCGCTTCGAAAGATTTCGGCCTGATGAGCAGAGCGGCGCCCTTGGCCGTTTTACACCCTGCCTCCGCCCAAGACGTGGCGGGACTGGTGCGGGCAGCCTACTCTTCGCCGCACGGGTTCACGGTCTCGGCCAGAGGCCACGGGCACTCCATCAACGGGCAGGCGCAGACCAACAACGGTGTCGTGATTCAGATGAGCGGGGGATCGTTGAGATCGGGGAGGCCGGCTCAGGCGGCTCGAGTATCGGAGAAGGGGATGTATGTTGATACGTGGGGTGGGGAGTTGTGGATAGATGTGCTCAGGAGCACGCTGGAGTATGGACTCGCACCGAAGTCGTGGACTGATTATTTGTACTTAACAGTCGGAGGAACCCTTTCCAACGCCGGAATCAGCGGCCAAGCTTTCAATTATGGACCTCAGATTAGTAATGTTCATGAGCTCGACGTTGTTACAGGCAAAGGCCAGCTATTGACATGTTCCGAAGAGCAAAACTCAGAGCTGTTCCAGGCTGTTCTTGGTGGGCTAGGCCAGTTTGGAATCATCACTAGGGCTAGAATTGCTCTCGAACCATCCCCCAAAAGGGTGAGGTGGATCCGAGTACTGTATTCCAATTTTTCGGCTTTTACCAAAGACCAGGAATTCCTCATCTCTCTGCATGGGCAACCTTCCAGCCAGAAATTCGACTATGTCGAGGGTTTCGTTATTGTCGACGAAGGCCTCATCAACAATTGGAGGTCATCTTTCTTCTCCGCTAGCAACCCTGTTAAAATCACCTCCATTGATTCTGAAGGTGGTGTTTTATATTGCTTGGAGATTACGAAAAATTACGACGAATCGACTGCCGATACAATTGACAAGGAAATAGAGGATTTGTTGGAAAAACTGGATTTTGTACCGACGTCAGTCTTCACAACTGATCTTCCATACGTGGATTTTCTGGACCGCGTTCACAAGGCCGAGTTGAAGCTCCGAGCCAAGGGTTTGTGGGACGTCCCCCATCCATGGCTTAACCTTTTTGTCCCCAAATCAAGGATTTCTGACTTCAACGAAGGAGTGTTCAAGGGCATTTTGGGAAATAAGACAAGCGGACCCATTCTGATTTACCCCATGAACAAAGACAAGTGGGACCAGAGTAGCTCGGTGGTGACGCCCGACGAGGACATTTTTTACCTTGTGGCGCTGCTAAGAACTGCCTTGGATACCGGGGACGAGACCCACACCTTGGAGCACCTGACCAATCAGAACCGTCAGATCCTCAGATTCTGTGTTGATGCGGGGATCGAGGTGAAACAGTACCTCCCGCACTATACTACGCAGGAGGAGTGGATGGACCACTTTGGAGATAAGTGGGCCCGGTTTTACAAGAGGAAGATGGAGTTCGACCCTCGGCGCATTTTAGCCACCGGCCAACGTATTTTCAGCCCTTCATCGGTTTCTTCTAGTCTTGGTGTTGGTGGTGATAAATTGTCGATTTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009308 GO:0009690 GO:0009823 GO:0009987 GO:0010817 GO:0016491 GO:0016645 GO:0019139 GO:0034754 GO:0042445 GO:0042447 GO:0044237 GO:0046483 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00908 map00908

Pfam Domains

Protein Families

Cytokin-bind FAD_binding_4

Protein Analysis

539

Amino Acids

59.53

Weight (kDa)

5.53

Isoelectric Point (pI)

37.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	60 - 206	4.1e-19	FAD binding domain
Cytokin-bind	PF09265	238 - 519	4.9e-127	Cytokinin dehydrogenase 1, FAD and cytokinin binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008662)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75450 AT1G75450
fragaria_vesca	FvH4_2g39230 FvH4_2g39230 FvH4_2g39230
malus_domestica	MD08G1023900.v1.1 MD15G1021500.v1.1
prunus_persica	Prupe.1G373300_v2.0.a1
pyrus_communis	pycom08g01870 pycom15g01780
rosa_chinensis	RchiOBHm_Chr6g0301781
rosa_laevigata	RLG00000011222
rosa_multiflora	Rmu_sc0005454.1_g000020
rosa_roxburghii	Rroxscaffold_7G00166410
rosa_rugosa	Rorug06G0309500 Rorug06G0309600 Rorug06G0309700 Rorug06G0309800
rosa_samantha	Rh6AG421500 Rh6BG428300 Rh6CG434800 Rh6DG421700
rosa_wichuraiana	Rw6G036500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	1040, 1131
AccB1I	GGYRCC	1 cut(s)	175
AccB7I	CCANNNNNTGG	2 cut(s)	214, 1360
AccBSI	CCGCTC	2 cut(s)	173, 336
AccI	GTMKAC	4 cut(s)	43, 102, 120, 846
AccII	CGCG	1 cut(s)	1083
AclWI	GGATC	7 cut(s)	68, 81, 349, 724, 737, 1384, 1424
AcoI	YGGCCR	4 cut(s)	183, 267, 532, 1543
AcsI	RAATTY	2 cut(s)	776, 815
AcuI	CTGAAG	1 cut(s)	945
AcyI	GRCGYC	4 cut(s)	176, 1037, 1128, 1286
AfaI	GTAC	4 cut(s)	491, 738, 1032, 1433
AfiI	CCNNNNNNNGG	9 cut(s)	214, 294, 717, 718, 1114, 1140, 1167, 1360, 1567
AflIII	ACRYGT	1 cut(s)	611
AgsI	TTSAA	4 cut(s)	544, 1102, 1183, 1198
AjiI	CACGTC	2 cut(s)	133, 214
AjnI	CCWGG	2 cut(s)	642, 771
AluBI	AGCT	9 cut(s)	16, 85, 112, 539, 577, 604, 637, 1105, 1275
AluI	AGCT	9 cut(s)	16, 85, 112, 539, 577, 604, 637, 1105, 1275
Alw21I	GWGCWC	4 cut(s)	438, 446, 579, 1368
Alw26I	GTCTC	2 cut(s)	268, 1343
AlwI	GGATC	7 cut(s)	68, 81, 349, 724, 737, 1384, 1424
AlwNI	CAGNNNCTG	1 cut(s)	1401
Ama87I	CYCGRG	1 cut(s)	375
ApaI	GGGCCC	1 cut(s)	1492
ApeKI	GCWGC	2 cut(s)	233, 1318
ApoI	RAATTY	2 cut(s)	776, 815
ArsI	GACNNNNNNTTYG	2 cut(s)	469, 501
Asp700I	GAANNNNTTC	2 cut(s)	152, 511
AspLEI	GCGC	4 cut(s)	178, 304, 1318, 1530
AspS9I	GGNCC	7 cut(s)	551, 1078, 1226, 1264, 1468, 1488, 1489
AsuC2I	CCSGG	2 cut(s)	1342, 1492
AsuHPI	GGTGA	5 cut(s)	733, 901, 1294, 1435, 1607
AsuII	TTCGAA	1 cut(s)	146
AsuNHI	GCTAGC	1 cut(s)	890
AvaI	CYCGRG	1 cut(s)	375
AvaII	GGWCC	5 cut(s)	551, 1078, 1226, 1264, 1468
BaeGI	GKGCMC	2 cut(s)	285, 1492
BamHI	GGATCC	2 cut(s)	73, 729
BanI	GGYRCC	1 cut(s)	175
BanII	GRGCYC	2 cut(s)	579, 1492
BbsI	GAAGAC	1 cut(s)	1036
Bbv12I	GWGCWC	4 cut(s)	438, 446, 579, 1368
BbvI	GCAGC	2 cut(s)	245, 1305
BccI	CCATC	6 cut(s)	133, 296, 715, 1143, 1459, 1504
BceAI	ACGGC	1 cut(s)	170
BcgI	CGANNNNNNTGC	4 cut(s)	453, 487, 959, 993
BciT130I	CCWGG	2 cut(s)	644, 773
BciVI	GTATCC	2 cut(s)	130, 1330
BcnI	CCSGG	2 cut(s)	1342, 1492
BcoDI	GTCTC	2 cut(s)	268, 1343
BfaI	CTAG	5 cut(s)	662, 684, 690, 891, 1578
BfoI	RGCGCY	2 cut(s)	179, 1319
BfuI	GTATCC	2 cut(s)	130, 1330
BisI	GCNGC	6 cut(s)	174, 234, 248, 372, 532, 1319
BlsI	GCNGC	6 cut(s)	175, 235, 249, 373, 533, 1320
BmcAI	AGTACT	1 cut(s)	738
Bme1390I	CCNGG	4 cut(s)	644, 773, 1342, 1492
Bme18I	GGWCC	5 cut(s)	551, 1078, 1226, 1264, 1468
BmeT110I	CYCGRG	1 cut(s)	375
BmgBI	CACGTC	2 cut(s)	133, 214
BmgT120I	GGNCC	7 cut(s)	551, 1078, 1226, 1264, 1468, 1488, 1489
BmiI	GGNNCC	7 cut(s)	75, 177, 508, 731, 1228, 1265, 1490
BmrFI	CCNGG	4 cut(s)	644, 773, 1342, 1492
BmsI	GCATC	1 cut(s)	1399
BmtI	GCTAGC	1 cut(s)	894
BoxI	GACNNNNGTC	1 cut(s)	1040
BpiI	GAAGAC	1 cut(s)	1036
BplI	GAGNNNNNCTC	4 cut(s)	349, 381, 614, 646
BpmI	CTGGAG	1 cut(s)	470
Bpu10I	CCTNAGC	2 cut(s)	366, 437
Bpu14I	TTCGAA	1 cut(s)	146
BpuMI	CCSGG	2 cut(s)	1342, 1492
BsaAI	YACGTR	2 cut(s)	408, 1066
BsaHI	GRCGYC	4 cut(s)	176, 1037, 1128, 1286
BsaI	GGTCTC	2 cut(s)	268, 1343
BsaJI	CCNNGG	9 cut(s)	69, 180, 277, 1113, 1139, 1332, 1341, 1359, 1523
BsaXI	ACNNNNNCTCC	6 cut(s)	185, 215, 269, 299, 1419, 1449
Bsc4I	CCNNNNNNNGG	9 cut(s)	214, 294, 717, 718, 1114, 1140, 1167, 1360, 1567
Bse118I	RCCGGY	2 cut(s)	361, 1541
Bse1I	ACTGG	3 cut(s)	228, 667, 1026
BseBI	CCWGG	2 cut(s)	644, 773
BseDI	CCNNGG	9 cut(s)	69, 180, 277, 1113, 1139, 1332, 1341, 1359, 1523
BseGI	GGATG	4 cut(s)	399, 707, 1135, 1470
BseLI	CCNNNNNNNGG	9 cut(s)	214, 294, 717, 718, 1114, 1140, 1167, 1360, 1567
BseMII	CTCAG	6 cut(s)	127, 380, 451, 569, 645, 1408
BseNI	ACTGG	3 cut(s)	228, 667, 1026
BseRI	GAGGAG	1 cut(s)	1472
BseSI	GKGCMC	2 cut(s)	285, 1492
BseXI	GCAGC	2 cut(s)	245, 1305
Bsh1236I	CGCG	1 cut(s)	1083
BshNI	GGYRCC	1 cut(s)	175
BsiHKAI	GWGCWC	4 cut(s)	438, 446, 579, 1368
BsiHKCI	CYCGRG	1 cut(s)	375
BsiSI	CCGG	5 cut(s)	362, 522, 1341, 1492, 1542
BslFI	GGGAC	6 cut(s)	234, 1115, 1139, 1142, 1277, 1358
BslI	CCNNNNNNNGG	9 cut(s)	214, 294, 717, 718, 1114, 1140, 1167, 1360, 1567
BsmAI	GTCTC	2 cut(s)	268, 1343
BsmFI	GGGAC	6 cut(s)	234, 1115, 1139, 1142, 1277, 1358
Bso31I	GGTCTC	2 cut(s)	268, 1343
BsoBI	CYCGRG	1 cut(s)	375
Bsp119I	TTCGAA	1 cut(s)	146
Bsp120I	GGGCCC	1 cut(s)	1488
Bsp1286I	GDGCHC	6 cut(s)	285, 438, 446, 579, 1368, 1492
Bsp143I	GATC	7 cut(s)	73, 341, 350, 729, 1054, 1389, 1416
Bsp19I	CCATGG	1 cut(s)	1139
BspCNI	CTCAG	6 cut(s)	126, 379, 450, 568, 644, 1407
BspFNI	CGCG	1 cut(s)	1083
BspHI	TCATGA	1 cut(s)	571
BspLI	GGNNCC	7 cut(s)	75, 177, 508, 731, 1228, 1265, 1490
BspOI	GCTAGC	1 cut(s)	894
BspPI	GGATC	7 cut(s)	68, 81, 349, 724, 737, 1384, 1424
BspQI	GCTCTTC	1 cut(s)	615
BspT104I	TTCGAA	1 cut(s)	146
BspT107I	GGYRCC	1 cut(s)	175
BspTNI	GGTCTC	2 cut(s)	268, 1343
BsrBI	CCGCTC	2 cut(s)	173, 336
BsrFI	RCCGGY	2 cut(s)	361, 1541
BsrI	ACTGG	3 cut(s)	228, 667, 1026
BssAI	RCCGGY	2 cut(s)	361, 1541
BssECI	CCNNGG	9 cut(s)	69, 180, 277, 1113, 1139, 1332, 1341, 1359, 1523
BssMI	GATC	7 cut(s)	73, 341, 350, 729, 1054, 1389, 1416
BssNI	GRCGYC	4 cut(s)	176, 1037, 1128, 1286
BssT1I	CCWWGG	5 cut(s)	180, 1113, 1139, 1332, 1359
Bst2UI	CCWGG	2 cut(s)	644, 773
Bst4CI	ACNGT	8 cut(s)	58, 70, 262, 317, 499, 741, 1385, 1431
Bst6I	CTCTTC	2 cut(s)	247, 615
BstACI	GRCGYC	4 cut(s)	176, 1037, 1128, 1286
BstBAI	YACGTR	2 cut(s)	408, 1066
BstBI	TTCGAA	1 cut(s)	146
BstC8I	GCNNGC	6 cut(s)	231, 300, 363, 446, 602, 892
BstDEI	CTNAG	8 cut(s)	113, 366, 437, 555, 631, 1322, 1394, 1614
BstDSI	CCRYGG	3 cut(s)	69, 277, 1139
BstF5I	GGATG	4 cut(s)	399, 707, 1135, 1470
BstFNI	CGCG	1 cut(s)	1083
BstH2I	RGCGCY	2 cut(s)	179, 1319
BstHHI	GCGC	4 cut(s)	178, 304, 1318, 1530
BstKTI	GATC	7 cut(s)	76, 344, 353, 732, 1057, 1392, 1419
BstMAI	GTCTC	2 cut(s)	268, 1343
BstMBI	GATC	7 cut(s)	73, 341, 350, 729, 1054, 1389, 1416
BstMWI	GCNNNNNNNGC	4 cut(s)	165, 182, 371, 695
BstNI	CCWGG	2 cut(s)	644, 773
BstNSI	RCATGY	1 cut(s)	615
BstPAI	GACNNNNGTC	1 cut(s)	1040
BstSCI	CCNGG	4 cut(s)	642, 771, 1340, 1490
BstSLI	GKGCMC	2 cut(s)	285, 1492
BstUI	CGCG	1 cut(s)	1083
BstV1I	GCAGC	2 cut(s)	245, 1305
BstV2I	GAAGAC	1 cut(s)	1036
BstX2I	RGATCY	3 cut(s)	73, 729, 1389
BstYI	RGATCY	3 cut(s)	73, 729, 1389
BsuI	GTATCC	2 cut(s)	130, 1330
BtgI	CCRYGG	3 cut(s)	69, 277, 1139
BtrI	CACGTC	2 cut(s)	133, 214
BtsCI	GGATG	4 cut(s)	399, 707, 1135, 1470
BtsIMutI	CAGTG	1 cut(s)	121
Cac8I	GCNNGC	6 cut(s)	231, 300, 363, 446, 602, 892
CaiI	CAGNNNCTG	1 cut(s)	1401
CciI	TCATGA	1 cut(s)	571
CfoI	GCGC	4 cut(s)	178, 304, 1318, 1530
Cfr10I	RCCGGY	2 cut(s)	361, 1541
Cfr13I	GGNCC	7 cut(s)	551, 1078, 1226, 1264, 1468, 1488, 1489
CseI	GACGC	1 cut(s)	1294
Csp6I	GTAC	4 cut(s)	490, 737, 1031, 1432
CviAII	CATG	5 cut(s)	572, 612, 794, 1140, 1246
CviQI	GTAC	4 cut(s)	490, 737, 1031, 1432
DdeI	CTNAG	8 cut(s)	113, 366, 437, 555, 631, 1322, 1394, 1614
DinI	GGCGCC	1 cut(s)	177
DpnI	GATC	7 cut(s)	75, 343, 352, 731, 1056, 1391, 1418
DpnII	GATC	7 cut(s)	73, 341, 350, 729, 1054, 1389, 1416
EaeI	YGGCCR	4 cut(s)	183, 267, 532, 1543
Eam1104I	CTCTTC	2 cut(s)	247, 615
EarI	CTCTTC	2 cut(s)	247, 615
EciI	GGCGGA	2 cut(s)	80, 193
Ecl136II	GAGCTC	1 cut(s)	577
Eco130I	CCWWGG	5 cut(s)	180, 1113, 1139, 1332, 1359
Eco147I	AGGCCT	1 cut(s)	855
Eco24I	GRGCYC	2 cut(s)	579, 1492
Eco31I	GGTCTC	2 cut(s)	268, 1343
Eco47I	GGWCC	5 cut(s)	551, 1078, 1226, 1264, 1468
Eco53kI	GAGCTC	1 cut(s)	577
Eco57I	CTGAAG	1 cut(s)	945
Eco88I	CYCGRG	1 cut(s)	375
EcoICRI	GAGCTC	1 cut(s)	577
EcoRI	GAATTC	1 cut(s)	776
EcoRII	CCWGG	2 cut(s)	642, 771
EcoT14I	CCWWGG	5 cut(s)	180, 1113, 1139, 1332, 1359
EcoT38I	GRGCYC	2 cut(s)	579, 1492
EgeI	GGCGCC	1 cut(s)	177
EheI	GGCGCC	1 cut(s)	177
ErhI	CCWWGG	5 cut(s)	180, 1113, 1139, 1332, 1359
FaeI	CATG	5 cut(s)	575, 615, 797, 1143, 1249
FaqI	GGGAC	6 cut(s)	234, 1115, 1139, 1142, 1277, 1358
FatI	CATG	5 cut(s)	571, 611, 793, 1139, 1245
FauI	CCCGC	5 cut(s)	211, 222, 329, 1405, 1446
FblI	GTMKAC	4 cut(s)	43, 102, 120, 846
Fnu4HI	GCNGC	6 cut(s)	174, 234, 248, 372, 532, 1319
FokI	GGATG	4 cut(s)	406, 694, 1122, 1477
FriOI	GRGCYC	2 cut(s)	579, 1492
Fsp4HI	GCNGC	6 cut(s)	174, 234, 248, 372, 532, 1319
FspBI	CTAG	5 cut(s)	662, 684, 690, 891, 1578
GlaI	GCGC	4 cut(s)	177, 303, 1317, 1529
GluI	GCNGC	6 cut(s)	174, 234, 248, 372, 532, 1319
GsuI	CTGGAG	1 cut(s)	470
HaeII	RGCGCY	2 cut(s)	179, 1319
HapII	CCGG	5 cut(s)	362, 522, 1341, 1492, 1542
HgaI	GACGC	1 cut(s)	1294
HhaI	GCGC	4 cut(s)	178, 304, 1318, 1530
Hin1I	GRCGYC	4 cut(s)	176, 1037, 1128, 1286
Hin1II	CATG	5 cut(s)	575, 615, 797, 1143, 1249
Hin6I	GCGC	4 cut(s)	176, 302, 1316, 1528
HinP1I	GCGC	4 cut(s)	176, 302, 1316, 1528
HincII	GTYRAC	3 cut(s)	44, 103, 847
HindII	GTYRAC	3 cut(s)	44, 103, 847
HindIII	AAGCTT	2 cut(s)	14, 537
HinfI	GANTC	7 cut(s)	325, 459, 525, 675, 920, 971, 1398
HpaII	CCGG	5 cut(s)	362, 522, 1341, 1492, 1542
HphI	GGTGA	5 cut(s)	733, 901, 1294, 1435, 1607
Hpy166II	GTNNAC	7 cut(s)	44, 103, 121, 258, 477, 847, 1087
Hpy188III	TCNNGA	5 cut(s)	322, 439, 572, 701, 1076
Hpy8I	GTNNAC	7 cut(s)	44, 103, 121, 258, 477, 847, 1087
Hpy99I	CGWCG	8 cut(s)	101, 104, 107, 584, 851, 971, 1039, 1295
HpyAV	CCTTC	6 cut(s)	382, 813, 845, 920, 1181, 1572
HpyCH4III	ACNGT	8 cut(s)	58, 70, 262, 317, 499, 741, 1385, 1431
HpyCH4IV	ACGT	8 cut(s)	132, 213, 407, 582, 1037, 1065, 1128, 1549
HpyCH4V	TGCA	1 cut(s)	793
HpyF10VI	GCNNNNNNNGC	4 cut(s)	165, 182, 371, 695
HpyF3I	CTNAG	8 cut(s)	113, 366, 437, 555, 631, 1322, 1394, 1614
HpySE526I	ACGT	8 cut(s)	132, 213, 407, 582, 1037, 1065, 1128, 1549
Hsp92I	GRCGYC	4 cut(s)	176, 1037, 1128, 1286
Hsp92II	CATG	5 cut(s)	575, 615, 797, 1143, 1249
HspAI	GCGC	4 cut(s)	176, 302, 1316, 1528
KasI	GGCGCC	1 cut(s)	175
KroI	GCCGGC	1 cut(s)	361
KroNI	GCCGGC	1 cut(s)	363
Kzo9I	GATC	7 cut(s)	73, 341, 350, 729, 1054, 1389, 1416
LguI	GCTCTTC	1 cut(s)	615
LmnI	GCTCC	4 cut(s)	82, 441, 1110, 1363
Lsp1109I	GCAGC	2 cut(s)	245, 1305
LweI	GCATC	1 cut(s)	1399
MaeI	CTAG	5 cut(s)	662, 684, 690, 891, 1578
MaeII	ACGT	8 cut(s)	132, 213, 407, 582, 1037, 1065, 1128, 1549
MaeIII	GTNAC	2 cut(s)	586, 1282
MalI	GATC	7 cut(s)	75, 343, 352, 731, 1056, 1391, 1418
MbiI	CCGCTC	2 cut(s)	173, 336
MboI	GATC	7 cut(s)	73, 341, 350, 729, 1054, 1389, 1416
MboII	GAAGA	7 cut(s)	234, 632, 874, 1036, 1049, 1519, 1566
MfeI	CAATTG	2 cut(s)	865, 987
MflI	RGATCY	3 cut(s)	73, 729, 1389
MhlI	GDGCHC	6 cut(s)	285, 438, 446, 579, 1368, 1492
Mly113I	GGCGCC	1 cut(s)	176
MlyI	GAGTC	1 cut(s)	453
MmeI	TCCRAC	4 cut(s)	101, 481, 540, 993
MroNI	GCCGGC	1 cut(s)	361
MroXI	GAANNNNTTC	2 cut(s)	152, 511
MseI	TTAA	5 cut(s)	23, 65, 494, 903, 1146
MspA1I	CMGCKG	1 cut(s)	531
MspI	CCGG	5 cut(s)	362, 522, 1341, 1492, 1542
MspR9I	CCNGG	4 cut(s)	644, 773, 1342, 1492
MunI	CAATTG	2 cut(s)	865, 987
MvaI	CCWGG	2 cut(s)	644, 773
MvnI	CGCG	1 cut(s)	1083
MwoI	GCNNNNNNNGC	4 cut(s)	165, 182, 371, 695
NaeI	GCCGGC	1 cut(s)	363
NarI	GGCGCC	1 cut(s)	176
NciI	CCSGG	2 cut(s)	1342, 1492
NcoI	CCATGG	1 cut(s)	1139
NdeII	GATC	7 cut(s)	73, 341, 350, 729, 1054, 1389, 1416
NgoMIV	GCCGGC	1 cut(s)	361
NheI	GCTAGC	1 cut(s)	890
NlaIII	CATG	5 cut(s)	575, 615, 797, 1143, 1249
NlaIV	GGNNCC	7 cut(s)	75, 177, 508, 731, 1228, 1265, 1490
NmeAIII	GCCGAG	3 cut(s)	245, 1120, 1504
NmuCI	GTSAC	1 cut(s)	1282
NspI	RCATGY	1 cut(s)	615
NspV	TTCGAA	1 cut(s)	146
PaeR7I	CTCGAG	1 cut(s)	375
PagI	TCATGA	1 cut(s)	571
PceI	AGGCCT	1 cut(s)	855
PciI	ACATGT	1 cut(s)	611
PciSI	GCTCTTC	1 cut(s)	615
PcsI	WCGNNNNNNNCGW	3 cut(s)	825, 834, 843
PdiI	GCCGGC	1 cut(s)	363
PdmI	GAANNNNTTC	2 cut(s)	152, 511
PfeI	GAWTC	6 cut(s)	325, 525, 675, 920, 971, 1398
PflFI	GACNNNGTC	1 cut(s)	824
PflMI	CCANNNNNTGG	2 cut(s)	214, 1360
PkrI	GCNGC	6 cut(s)	175, 235, 249, 373, 533, 1320
PleI	GAGTC	1 cut(s)	453
PluTI	GGCGCC	1 cut(s)	179
PpsI	GAGTC	1 cut(s)	453
Ppu21I	YACGTR	2 cut(s)	408, 1066
PscI	ACATGT	1 cut(s)	611
PshAI	GACNNNNGTC	1 cut(s)	1040
Psp124BI	GAGCTC	1 cut(s)	579
Psp6I	CCWGG	2 cut(s)	642, 771
PspGI	CCWGG	2 cut(s)	642, 771
PspN4I	GGNNCC	7 cut(s)	75, 177, 508, 731, 1228, 1265, 1490
PspOMI	GGGCCC	1 cut(s)	1488
PspPI	GGNCC	7 cut(s)	551, 1078, 1226, 1264, 1468, 1488, 1489
PspXI	VCTCGAGB	1 cut(s)	375
PstNI	CAGNNNCTG	1 cut(s)	1401
PsuI	RGATCY	3 cut(s)	73, 729, 1389
PsyI	GACNNNGTC	1 cut(s)	824
RsaI	GTAC	4 cut(s)	491, 738, 1032, 1433
RsaNI	GTAC	4 cut(s)	490, 737, 1031, 1432
SacI	GAGCTC	1 cut(s)	579
SalI	GTCGAC	3 cut(s)	42, 101, 845
SapI	GCTCTTC	1 cut(s)	615
SaqAI	TTAA	5 cut(s)	23, 65, 494, 903, 1146
SatI	GCNGC	6 cut(s)	174, 234, 248, 372, 532, 1319
Sau3AI	GATC	7 cut(s)	73, 341, 350, 729, 1054, 1389, 1416
Sau96I	GGNCC	7 cut(s)	551, 1078, 1226, 1264, 1468, 1488, 1489
ScaI	AGTACT	1 cut(s)	738
SchI	GAGTC	1 cut(s)	453
ScrFI	CCNGG	4 cut(s)	644, 773, 1342, 1492
SduI	GDGCHC	6 cut(s)	285, 438, 446, 579, 1368, 1492
SfaNI	GCATC	1 cut(s)	1399
SfoI	GGCGCC	1 cut(s)	177
Sfr274I	CTCGAG	1 cut(s)	375
SfuI	TTCGAA	1 cut(s)	146
SgrDI	CGTCGACG	1 cut(s)	101
SinI	GGWCC	5 cut(s)	551, 1078, 1226, 1264, 1468
SlaI	CTCGAG	1 cut(s)	375
SmlI	CTYRAG	1 cut(s)	375
SmoI	CTYRAG	1 cut(s)	375
SseBI	AGGCCT	1 cut(s)	855
SspDI	GGCGCC	1 cut(s)	175
SspMI	CTAG	5 cut(s)	662, 684, 690, 891, 1578
SstI	GAGCTC	1 cut(s)	579
StuI	AGGCCT	1 cut(s)	855
StyD4I	CCNGG	4 cut(s)	642, 771, 1340, 1490
StyI	CCWWGG	5 cut(s)	180, 1113, 1139, 1332, 1359
TaaI	ACNGT	8 cut(s)	58, 70, 262, 317, 499, 741, 1385, 1431
TaiI	ACGT	8 cut(s)	135, 216, 410, 585, 1040, 1068, 1131, 1552
TatI	WGTACW	2 cut(s)	489, 736
TauI	GCSGC	4 cut(s)	176, 250, 374, 534
TfiI	GAWTC	6 cut(s)	325, 525, 675, 920, 971, 1398
Tru1I	TTAA	5 cut(s)	23, 65, 494, 903, 1146
Tru9I	TTAA	5 cut(s)	23, 65, 494, 903, 1146
TscAI	CASTG	1 cut(s)	121
TseFI	GTSAC	1 cut(s)	1282
TseI	GCWGC	2 cut(s)	233, 1318
Tsp45I	GTSAC	1 cut(s)	1282
TspDTI	ATGAA	3 cut(s)	560, 1262, 1554
TspGWI	ACGGA	2 cut(s)	95, 120
TspRI	CASTG	1 cut(s)	121
Tth111I	GACNNNGTC	1 cut(s)	824
Van91I	CCANNNNNTGG	2 cut(s)	214, 1360
VpaK11BI	GGWCC	5 cut(s)	551, 1078, 1226, 1264, 1468
XapI	RAATTY	2 cut(s)	776, 815
XceI	RCATGY	1 cut(s)	615
XcmI	CCANNNNNNNNNTGG	1 cut(s)	650
XhoI	CTCGAG	1 cut(s)	375
XmiI	GTMKAC	4 cut(s)	43, 102, 120, 846
XmnI	GAANNNNTTC	2 cut(s)	152, 511
XspI	CTAG	5 cut(s)	662, 684, 690, 891, 1578
ZraI	GACGTC	2 cut(s)	1038, 1129
ZrmI	AGTACT	1 cut(s)	738

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.