MD08G1029000.v1.1

Adenosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015120
Physical Location & Seq
Reverse (-)
2112272 .. 2115024
2753 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1029000.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGGGTGGAGAAACCTTACCCGCCACAGCCAACCCCGCCGCGCGAGTGGCTCCTCTGGTACTGGGACTACAGGCTTCCGCCCTCGTCGACCATGTTGCCCGCGTTGACTGGTCCCTGCTCGATCAACTCCCCGGCGAGCGCGGTGGCTCCATTCCTGTTGAAATTGAAGAGCTTGAGCATATATTGAGTGAGGTGAAAACCCATGTAATTTCATCCCCTGATGATTCCTTGCCAATGAAAACCATCGCCGGTGGCAGTGTTGCCAATACAATCCGAGGGCTGAGTGCCGGTTTCGGAGTCTCCTGCGGGATAATTGGTGCCTGTGGAGATGATGAGCAAGGCCAGTTATTTGTCAGTAACATGAGCTCTCATGCTGTGAACCTCTCTAGATTGAGGATGAAGAAGGGACCCACAGCTCAGTGTGTTTGCTTGGTCGATGCTTTGGGCAATCGGACAATGCGTCCCTGTCTCTCAAGTGCTGTGAAACTTCAGTCTCAGGCTGATGACTTCACGAGAGCAGACTTCAAGGGCTCTAAGTGGTTGTTGTTGAGGTATGGTATCATCAATTTGGAAGTGATTCAAGTGGCTCTATCGATTGCGAAACAAGAGGGTCTATTTGTGTCCTTAGACTTGGCCAGTTTTGAGATGGTTCGCAACTTTAGATCGCCTCTTCTGCAGCTGCTGGAGTCAGGGGACATAGACCTCTGCTTTGCCAACGAGGATGAAGCAGCAGAGTTGCTAAGAGGTTCCGAGGGTGAACCACAGGCTGATCCAGAGGTTGCACTTGAGTTTCTGGGGAAACACTGCCGCTGGGCTGTGGTGACGTTAGGCTCCAACGGATGTATAGCAAAGCATGGAAAAGAGATTGTGAGAGTTCCAGCCATAGGGGAAGCCAATGCAGTCGATGCCACAGGAGCAGGAGACCTGTTTGCGAGTGGATTTTTATATGGATTGGTGAAGGGATTATCTCTGGAGGAATGCTGCAAAGTAGGATCGTGCAGTGGCGGATCTGTTATTCGATCTCTCGGGGGTGAGGTCACCCCCGAGAACTGGCAATGGATGTACAAGCAGATGCAGACCAAAGGCCTCACTCTTCCTCATATCCCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

371

Amino Acids

39.31

Weight (kDa)

5.2

Isoelectric Point (pI)

39.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 80 - 344 4.2e-39 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015120)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19600
fragaria_vesca FvH4_2g39580 FvH4_2g39580 FvH4_2g39580
malus_domestica MD08G1029000.v1.1 MD15G1024500.v1.1
prunus_persica Prupe.1G376800_v2.0.a1 Prupe.1G376800_v2.0.a1
pyrus_communis pycom08g02280
rosa_chinensis RchiOBHm_Chr6g0301341
rosa_laevigata RLG00000011259
rosa_multiflora Rmu_sc0013354.1_g000023
rosa_roxburghii Rroxscaffold_7G00166900
rosa_rugosa Rorug06G0303800
rosa_samantha Rh6AG417400
rosa_wichuraiana Rw6G036120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 317
AccI GTMKAC 1 cut(s) 87
AccII CGCG 4 cut(s) 41, 43, 102, 141
AciI CCGC 9 cut(s) 21, 36, 39, 78, 100, 141, 306, 808, 1005
AclWI GGATC 3 cut(s) 764, 1000, 1015
AcoI YGGCCR 1 cut(s) 633
AcuI CTGAAG 1 cut(s) 473
AfaI GTAC 2 cut(s) 60, 1064
AfiI CCNNNNNNNGG 2 cut(s) 884, 1050
AgsI TTSAA 4 cut(s) 161, 167, 526, 581
AhdI GACNNNNNGTC 1 cut(s) 459
AluBI AGCT 4 cut(s) 172, 366, 416, 679
AluI AGCT 4 cut(s) 172, 366, 416, 679
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 4 cut(s) 304, 473, 498, 915
AlwI GGATC 3 cut(s) 764, 1000, 1015
AlwNI CAGNNNCTG 1 cut(s) 682
Ama87I CYCGRG 2 cut(s) 1025, 1043
AoxI GGCC 3 cut(s) 340, 633, 1084
ApeKI GCWGC 4 cut(s) 676, 679, 728, 981
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
Asp700I GAANNNNTTC 1 cut(s) 576
AspLEI GCGC 2 cut(s) 43, 141
AspS9I GGNCC 2 cut(s) 111, 407
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 6 cut(s) 205, 767, 832, 967, 1030, 1043
AvaI CYCGRG 2 cut(s) 1025, 1043
AvaII GGWCC 2 cut(s) 111, 407
BalI TGGCCA 1 cut(s) 635
BanI GGYRCC 1 cut(s) 317
BanII GRGCYC 2 cut(s) 368, 533
BauI CACGAG 1 cut(s) 511
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 4 cut(s) 666, 688, 740, 968
BccI CCATC 2 cut(s) 251, 640
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 4 cut(s) 304, 473, 498, 915
BfaI CTAG 1 cut(s) 387
BfmI CTRYAG 2 cut(s) 68, 674
BisI GCNGC 6 cut(s) 39, 677, 680, 729, 808, 982
BlsI GCNGC 6 cut(s) 40, 678, 681, 730, 809, 983
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 2 cut(s) 111, 407
BmeRI GACNNNNNGTC 1 cut(s) 459
BmeT110I CYCGRG 2 cut(s) 1025, 1043
BmgT120I GGNCC 2 cut(s) 111, 407
BmiI GGNNCC 8 cut(s) 51, 113, 148, 319, 408, 409, 748, 832
BmrFI CCNGG 1 cut(s) 132
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 3 cut(s) 427, 895, 1062
BmuI ACTGGG 1 cut(s) 71
BpmI CTGGAG 2 cut(s) 704, 992
BpuEI CTTGAG 3 cut(s) 194, 457, 806
BpuMI CCSGG 1 cut(s) 132
Bsa29I ATCGAT 1 cut(s) 593
BsaI GGTCTC 1 cut(s) 915
BsaJI CCNNGG 3 cut(s) 130, 274, 750
Bsc4I CCNNNNNNNGG 2 cut(s) 884, 1050
Bse118I RCCGGY 2 cut(s) 248, 287
Bse1I ACTGG 5 cut(s) 66, 113, 343, 636, 1055
Bse3DI GCAATG 1 cut(s) 1061
BseCI ATCGAT 1 cut(s) 593
BseDI CCNNGG 3 cut(s) 130, 274, 750
BseGI GGATG 5 cut(s) 212, 402, 727, 845, 1065
BseLI CCNNNNNNNGG 2 cut(s) 884, 1050
BseMI GCAATG 1 cut(s) 1061
BseMII CTCAG 3 cut(s) 272, 431, 509
BseNI ACTGG 5 cut(s) 66, 113, 343, 636, 1055
BseRI GAGGAG 1 cut(s) 42
BseXI GCAGC 4 cut(s) 666, 688, 740, 968
BseYI CCCAGC 1 cut(s) 810
BsgI GTGCAG 1 cut(s) 1018
Bsh1236I CGCG 4 cut(s) 41, 43, 102, 141
BshFI GGCC 3 cut(s) 342, 635, 1086
BshNI GGYRCC 1 cut(s) 317
BshVI ATCGAT 1 cut(s) 593
BsiHKAI GWGCWC 1 cut(s) 368
BsiHKCI CYCGRG 2 cut(s) 1025, 1043
BsiSI CCGG 3 cut(s) 132, 249, 288
BslFI GGGAC 5 cut(s) 78, 97, 420, 447, 707
BslI CCNNNNNNNGG 2 cut(s) 884, 1050
BsmAI GTCTC 4 cut(s) 304, 473, 498, 915
BsmFI GGGAC 5 cut(s) 78, 97, 420, 447, 707
BsmI GAATGC 1 cut(s) 983
BsnI GGCC 3 cut(s) 342, 635, 1086
Bso31I GGTCTC 1 cut(s) 915
BsoBI CYCGRG 2 cut(s) 1025, 1043
Bsp1286I GDGCHC 2 cut(s) 368, 533
Bsp1407I TGTACA 1 cut(s) 1062
Bsp143I GATC 6 cut(s) 121, 662, 769, 992, 1007, 1019
BspACI CCGC 9 cut(s) 21, 36, 39, 78, 100, 141, 306, 808, 1005
BspANI GGCC 3 cut(s) 342, 635, 1086
BspCNI CTCAG 3 cut(s) 273, 430, 508
BspDI ATCGAT 1 cut(s) 593
BspFNI CGCG 4 cut(s) 41, 43, 102, 141
BspLI GGNNCC 8 cut(s) 51, 113, 148, 319, 408, 409, 748, 832
BspMAI CTGCAG 1 cut(s) 678
BspPI GGATC 3 cut(s) 764, 1000, 1015
BspQI GCTCTTC 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 915
BsrDI GCAATG 1 cut(s) 1061
BsrFI RCCGGY 2 cut(s) 248, 287
BsrGI TGTACA 1 cut(s) 1062
BsrI ACTGG 5 cut(s) 66, 113, 343, 636, 1055
BssAI RCCGGY 2 cut(s) 248, 287
BssECI CCNNGG 3 cut(s) 130, 274, 750
BssMI GATC 6 cut(s) 121, 662, 769, 992, 1007, 1019
BssSI CACGAG 1 cut(s) 511
Bst2BI CACGAG 1 cut(s) 511
Bst6I CTCTTC 3 cut(s) 162, 675, 1098
BstAPI GCANNNNNTGC 1 cut(s) 905
BstAUI TGTACA 1 cut(s) 1062
BstC8I GCNNGC 2 cut(s) 100, 137
BstDEI CTNAG 6 cut(s) 281, 417, 495, 534, 625, 740
BstEII GGTNACC 1 cut(s) 1036
BstF5I GGATG 5 cut(s) 212, 402, 727, 845, 1065
BstFNI CGCG 4 cut(s) 41, 43, 102, 141
BstHHI GCGC 2 cut(s) 43, 141
BstKTI GATC 6 cut(s) 124, 665, 772, 995, 1010, 1022
BstMAI GTCTC 4 cut(s) 304, 473, 498, 915
BstMBI GATC 6 cut(s) 121, 662, 769, 992, 1007, 1019
BstMWI GCNNNNNNNGC 5 cut(s) 35, 47, 673, 905, 914
BstPI GGTNACC 1 cut(s) 1036
BstSCI CCNGG 1 cut(s) 130
BstSFI CTRYAG 2 cut(s) 68, 674
BstUI CGCG 4 cut(s) 41, 43, 102, 141
BstV1I GCAGC 4 cut(s) 666, 688, 740, 968
BstX2I RGATCY 1 cut(s) 1007
BstYI RGATCY 1 cut(s) 1007
Bsu15I ATCGAT 1 cut(s) 593
BsuRI GGCC 3 cut(s) 342, 635, 1086
BsuTUI ATCGAT 1 cut(s) 593
BtgZI GCGATG 1 cut(s) 229
BtsCI GGATG 5 cut(s) 212, 402, 727, 845, 1065
BtsI GCAGTG 3 cut(s) 262, 802, 1006
BtsIMutI CAGTG 4 cut(s) 262, 425, 802, 1006
Cac8I GCNNGC 2 cut(s) 100, 137
CaiI CAGNNNCTG 1 cut(s) 682
CfoI GCGC 2 cut(s) 43, 141
Cfr10I RCCGGY 2 cut(s) 248, 287
Cfr13I GGNCC 2 cut(s) 111, 407
ClaI ATCGAT 1 cut(s) 593
CseI GACGC 1 cut(s) 449
Csp6I GTAC 2 cut(s) 59, 1063
CviAII CATG 5 cut(s) 92, 203, 361, 371, 854
CviQI GTAC 2 cut(s) 59, 1063
DdeI CTNAG 6 cut(s) 281, 417, 495, 534, 625, 740
DpnI GATC 6 cut(s) 123, 664, 771, 994, 1009, 1021
DpnII GATC 6 cut(s) 121, 662, 769, 992, 1007, 1019
DriI GACNNNNNGTC 1 cut(s) 459
EaeI YGGCCR 1 cut(s) 633
Eam1104I CTCTTC 3 cut(s) 162, 675, 1098
Eam1105I GACNNNNNGTC 1 cut(s) 459
EarI CTCTTC 3 cut(s) 162, 675, 1098
EciI GGCGGA 2 cut(s) 67, 1020
Ecl136II GAGCTC 1 cut(s) 366
Eco147I AGGCCT 1 cut(s) 1086
Eco24I GRGCYC 2 cut(s) 368, 533
Eco31I GGTCTC 1 cut(s) 915
Eco47I GGWCC 2 cut(s) 111, 407
Eco53kI GAGCTC 1 cut(s) 366
Eco57I CTGAAG 1 cut(s) 473
Eco88I CYCGRG 2 cut(s) 1025, 1043
Eco91I GGTNACC 1 cut(s) 1036
EcoICRI GAGCTC 1 cut(s) 366
EcoO109I RGGNCCY 1 cut(s) 407
EcoO65I GGTNACC 1 cut(s) 1036
EcoT38I GRGCYC 2 cut(s) 368, 533
FaeI CATG 5 cut(s) 95, 206, 364, 374, 857
FaqI GGGAC 5 cut(s) 78, 97, 420, 447, 707
FatI CATG 5 cut(s) 91, 202, 360, 370, 853
FauI CCCGC 4 cut(s) 28, 43, 107, 299
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 6 cut(s) 39, 677, 680, 729, 808, 982
FokI GGATG 5 cut(s) 199, 409, 734, 852, 1072
FriOI GRGCYC 2 cut(s) 368, 533
Fsp4HI GCNGC 6 cut(s) 39, 677, 680, 729, 808, 982
FspBI CTAG 1 cut(s) 387
GlaI GCGC 2 cut(s) 42, 140
GluI GCNGC 6 cut(s) 39, 677, 680, 729, 808, 982
GsaI CCCAGC 1 cut(s) 814
GsuI CTGGAG 2 cut(s) 704, 992
HaeIII GGCC 3 cut(s) 342, 635, 1086
HapII CCGG 3 cut(s) 132, 249, 288
HgaI GACGC 1 cut(s) 449
HhaI GCGC 2 cut(s) 43, 141
Hin1II CATG 5 cut(s) 95, 206, 364, 374, 857
Hin6I GCGC 2 cut(s) 41, 139
HinP1I GCGC 2 cut(s) 41, 139
HincII GTYRAC 2 cut(s) 88, 106
HindII GTYRAC 2 cut(s) 88, 106
HinfI GANTC 4 cut(s) 224, 297, 577, 686
HpaII CCGG 3 cut(s) 132, 249, 288
HphI GGTGA 6 cut(s) 205, 767, 832, 967, 1030, 1043
Hpy166II GTNNAC 4 cut(s) 88, 106, 379, 758
Hpy188I TCNGA 4 cut(s) 275, 296, 453, 751
Hpy188III TCNNGA 4 cut(s) 387, 511, 773, 971
Hpy8I GTNNAC 4 cut(s) 88, 106, 379, 758
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 2 cut(s) 397, 952
HpyCH4IV ACGT 1 cut(s) 824
HpyCH4V TGCA 6 cut(s) 676, 782, 899, 984, 999, 1075
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 47, 673, 905, 914
HpyF3I CTNAG 6 cut(s) 281, 417, 495, 534, 625, 740
HpySE526I ACGT 1 cut(s) 824
Hsp92II CATG 5 cut(s) 95, 206, 364, 374, 857
HspAI GCGC 2 cut(s) 41, 139
KflI GGGWCCC 1 cut(s) 407
Kzo9I GATC 6 cut(s) 121, 662, 769, 992, 1007, 1019
LguI GCTCTTC 1 cut(s) 162
LmnI GCTCC 4 cut(s) 55, 152, 836, 914
Lsp1109I GCAGC 4 cut(s) 666, 688, 740, 968
LweI GCATC 3 cut(s) 427, 895, 1062
MaeI CTAG 1 cut(s) 387
MaeII ACGT 1 cut(s) 824
MaeIII GTNAC 3 cut(s) 356, 820, 1036
MalI GATC 6 cut(s) 123, 664, 771, 994, 1009, 1021
MboI GATC 6 cut(s) 121, 662, 769, 992, 1007, 1019
MboII GAAGA 4 cut(s) 179, 412, 662, 1085
MflI RGATCY 1 cut(s) 1007
MhlI GDGCHC 2 cut(s) 368, 533
MlsI TGGCCA 1 cut(s) 635
MluCI AATT 4 cut(s) 162, 207, 312, 565
MluNI TGGCCA 1 cut(s) 635
MlyI GAGTC 2 cut(s) 306, 695
MmeI TCCRAC 1 cut(s) 858
Mox20I TGGCCA 1 cut(s) 635
MroXI GAANNNNTTC 1 cut(s) 576
MscI TGGCCA 1 cut(s) 635
Msp20I TGGCCA 1 cut(s) 635
MspA1I CMGCKG 2 cut(s) 679, 810
MspI CCGG 3 cut(s) 132, 249, 288
MspR9I CCNGG 1 cut(s) 132
Mva1269I GAATGC 1 cut(s) 983
MvnI CGCG 4 cut(s) 41, 43, 102, 141
MwoI GCNNNNNNNGC 5 cut(s) 35, 47, 673, 905, 914
NciI CCSGG 1 cut(s) 132
NdeII GATC 6 cut(s) 121, 662, 769, 992, 1007, 1019
NlaIII CATG 5 cut(s) 95, 206, 364, 374, 857
NlaIV GGNNCC 8 cut(s) 51, 113, 148, 319, 408, 409, 748, 832
NmuCI GTSAC 2 cut(s) 820, 1036
PceI AGGCCT 1 cut(s) 1086
PciSI GCTCTTC 1 cut(s) 162
PcsI WCGNNNNNNNCGW 1 cut(s) 457
PctI GAATGC 1 cut(s) 983
PdmI GAANNNNTTC 1 cut(s) 576
PfeI GAWTC 2 cut(s) 224, 577
PkrI GCNGC 6 cut(s) 40, 678, 681, 730, 809, 983
PleI GAGTC 2 cut(s) 305, 694
PpsI GAGTC 2 cut(s) 305, 694
PpuMI RGGWCCY 1 cut(s) 407
Psp124BI GAGCTC 1 cut(s) 368
Psp5II RGGWCCY 1 cut(s) 407
PspEI GGTNACC 1 cut(s) 1036
PspFI CCCAGC 1 cut(s) 810
PspN4I GGNNCC 8 cut(s) 51, 113, 148, 319, 408, 409, 748, 832
PspPI GGNCC 2 cut(s) 111, 407
PspPPI RGGWCCY 1 cut(s) 407
PstI CTGCAG 1 cut(s) 678
PstNI CAGNNNCTG 1 cut(s) 682
PsuI RGATCY 1 cut(s) 1007
PvuII CAGCTG 1 cut(s) 679
RsaI GTAC 2 cut(s) 60, 1064
RsaNI GTAC 2 cut(s) 59, 1063
SacI GAGCTC 1 cut(s) 368
SalI GTCGAC 1 cut(s) 86
SapI GCTCTTC 1 cut(s) 162
SatI GCNGC 6 cut(s) 39, 677, 680, 729, 808, 982
Sau3AI GATC 6 cut(s) 121, 662, 769, 992, 1007, 1019
Sau96I GGNCC 2 cut(s) 111, 407
SchI GAGTC 2 cut(s) 306, 695
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 2 cut(s) 368, 533
SfaNI GCATC 3 cut(s) 427, 895, 1062
SfcI CTRYAG 2 cut(s) 68, 674
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 2 cut(s) 111, 407
SmlI CTYRAG 3 cut(s) 173, 472, 785
SmoI CTYRAG 3 cut(s) 173, 472, 785
Sse9I AATT 4 cut(s) 162, 207, 312, 565
SseBI AGGCCT 1 cut(s) 1086
SsiI CCGC 9 cut(s) 21, 36, 39, 78, 100, 141, 306, 808, 1005
SspMI CTAG 1 cut(s) 387
SstI GAGCTC 1 cut(s) 368
StuI AGGCCT 1 cut(s) 1086
StyD4I CCNGG 1 cut(s) 130
TaiI ACGT 1 cut(s) 827
TaqI TCGA 6 cut(s) 87, 120, 435, 593, 903, 1018
TasI AATT 4 cut(s) 162, 207, 312, 565
TatI WGTACW 1 cut(s) 1062
TauI GCSGC 2 cut(s) 41, 810
TfiI GAWTC 2 cut(s) 224, 577
TscAI CASTG 4 cut(s) 262, 425, 809, 1006
TseFI GTSAC 2 cut(s) 820, 1036
TseI GCWGC 4 cut(s) 676, 679, 728, 981
Tsp45I GTSAC 2 cut(s) 820, 1036
TspDTI ATGAA 4 cut(s) 201, 251, 413, 738
TspGWI ACGGA 1 cut(s) 852
TspRI CASTG 4 cut(s) 262, 425, 809, 1006
VpaK11BI GGWCC 2 cut(s) 111, 407
XbaI TCTAGA 1 cut(s) 386
XcmI CCANNNNNNNNNTGG 1 cut(s) 643
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.