Prupe.1G376800_v2.0.a1

Adenosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0015120
Physical Location & Seq
Reverse (-)
34136226 .. 34139479
3254 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G376800.2

Sequence Viewer

Length: 1221 bp
ATGACATCGAAAAACTTCAAGTGTGAAGAGAGAGCGAGAGCAAAGTCCCAATTCCCAAAGACCAGAGCCAGAGGGCCGAAAATGGGAGCAGAAGCCTTGCCCGTCACCGCCAACTCCAACAACAACTGCAGCGGAGGCGAGGCTCCTCTGGTATTGGGACTACAGGCATCCGCCCTCGTCGACCATGTGGCCCGCGTCGATTGGTCCTTGCTCGATCAACTCCCAGGCGAGCGCGGTGGCTCCATTCCTGTTGCAATTGAAGAGCTTGAGCACATACTGGGTGAGGTGAAGACCCATATTATCTCATCCCCTGATGATTCCTTGCCCATGAAAACAATAGCCGGGGGCAGTGTAGCCAATACTATCAGAGGGCTGAGTGCTGGGTTCGGAATCTCCTGTGGAATAATTGGTGCCTGTGGGGATGATGAGCAAGGCCAGTTATTTGTCAGTAACATGAGCTCTCATGCTGTGAGCCTCTCAAGATTGAGGATGAAAAAGGGACCCACAGCTCAGTGCGTTTGCTTGGTTGATGCCTTGGGCAATCGTACAATGCGTCCCTGTCTCTCAAGTGCCGTGAAACTTCACCCTCAGAATTATGTTATTCAGGCAGATGACTTGACAAGAGCGGATTTTAAGGGCTGTAAGTGGCTGTTGTTGAGGTATGGTATTATCAATTTGGAAGTGATTCAAGCTGCTATACGGATTGCCAAACAAGAGGGTCTTTTTGTGTCCATGGACTTGGCTAGCTTTGAGATGGTTCGCAACTTTAGATCACCTCTTCTGCAGTTGCTGGAGTCAGGGGGCATAGACCTCTGCTTTGCTAATGAGGATGAAGCAACAGAGTTGCTAAGATGCACTGAGGGTGAACAAAAAGCTGATCCTGAGGCTGCACTTGAATTTCTGGCAAAACATTGCCGGTGGGCAGTGGTGACGTTAGGCCCCAATGGATGCATTGCAAAGCACGGAAAAGAGATTGTGAGAGTTCCAGCCATAGGGAAAGCCAATGCAGTGGATGCCACAGGAGCAGGAGACCTGTTTGCAAGTGGATTTTTGTATGGATTGGTGAAGGGACTATCTCTGGAGGAATGCTGCAAAGTGGGATCATGCAGTGGCGGATCTGTTATTCGCTCTCTCGGGGGCGAGGTCACCCCTGAGAATTGGCAATGGATGTACAAGCAGATGCAGACCAAGGGCCTCACTCTTCCTGATATCCCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

407

Amino Acids

43.46

Weight (kDa)

6.67

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015120)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19600
fragaria_vesca FvH4_2g39580 FvH4_2g39580 FvH4_2g39580
malus_domestica MD08G1029000.v1.1 MD15G1024500.v1.1
prunus_persica Prupe.1G376800_v2.0.a1 Prupe.1G376800_v2.0.a1
pyrus_communis pycom08g02280
rosa_chinensis RchiOBHm_Chr6g0301341
rosa_laevigata RLG00000011259
rosa_multiflora Rmu_sc0013354.1_g000023
rosa_roxburghii Rroxscaffold_7G00166900
rosa_rugosa Rorug06G0303800
rosa_samantha Rh6AG417400
rosa_wichuraiana Rw6G036120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 410
AccBSI CCGCTC 1 cut(s) 626
AccI GTMKAC 1 cut(s) 180
AccII CGCG 2 cut(s) 195, 234
AciI CCGC 7 cut(s) 108, 132, 171, 193, 234, 626, 1113
AclWI GGATC 3 cut(s) 872, 1108, 1123
AcsI RAATTY 1 cut(s) 896
AfaI GTAC 2 cut(s) 547, 1172
AfiI CCNNNNNNNGG 2 cut(s) 83, 992
AgsI TTSAA 4 cut(s) 19, 260, 689, 896
AjnI CCWGG 1 cut(s) 223
AluBI AGCT 6 cut(s) 265, 459, 509, 692, 747, 875
AluI AGCT 6 cut(s) 265, 459, 509, 692, 747, 875
Alw21I GWGCWC 2 cut(s) 273, 461
Alw26I GTCTC 2 cut(s) 566, 1023
AlwI GGATC 3 cut(s) 872, 1108, 1123
AlwNI CAGNNNCTG 1 cut(s) 790
Ama87I CYCGRG 1 cut(s) 1133
AoxI GGCC 5 cut(s) 74, 189, 433, 937, 1192
ApeKI GCWGC 4 cut(s) 129, 692, 887, 1089
ApoI RAATTY 1 cut(s) 896
ArsI GACNNNNNNTTYG 2 cut(s) 800, 832
Asp700I GAANNNNTTC 2 cut(s) 14, 684
AspLEI GCGC 1 cut(s) 234
AspS9I GGNCC 6 cut(s) 74, 190, 204, 500, 938, 1192
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 9 cut(s) 97, 293, 298, 575, 765, 875, 940, 1075, 1138
AsuNHI GCTAGC 1 cut(s) 743
AvaI CYCGRG 1 cut(s) 1133
AvaII GGWCC 2 cut(s) 204, 500
AxyI CCTNAGG 1 cut(s) 882
BanI GGYRCC 1 cut(s) 410
BanII GRGCYC 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 2 cut(s) 273, 461
BbvI GCAGC 4 cut(s) 141, 679, 874, 1076
BccI CCATC 1 cut(s) 748
BceAI ACGGC 1 cut(s) 557
BciT130I CCWGG 1 cut(s) 225
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 2 cut(s) 566, 1023
BfaI CTAG 1 cut(s) 744
BfmI CTRYAG 3 cut(s) 127, 161, 782
BisI GCNGC 4 cut(s) 130, 693, 888, 1090
BlsI GCNGC 4 cut(s) 131, 694, 889, 1091
Bme1390I CCNGG 2 cut(s) 225, 343
Bme18I GGWCC 2 cut(s) 204, 500
BmeT110I CYCGRG 1 cut(s) 1133
BmgT120I GGNCC 6 cut(s) 74, 190, 204, 500, 938, 1192
BmiI GGNNCC 6 cut(s) 144, 241, 412, 501, 502, 940
BmrFI CCNGG 2 cut(s) 225, 343
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 6 cut(s) 176, 520, 842, 938, 1003, 1170
BmtI GCTAGC 1 cut(s) 747
BmuI ACTGGG 1 cut(s) 287
BpiI GAAGAC 1 cut(s) 296
BpmI CTGGAG 2 cut(s) 812, 1100
BpuEI CTTGAG 3 cut(s) 287, 463, 550
BpuMI CCSGG 1 cut(s) 343
BsaI GGTCTC 1 cut(s) 1023
BsaJI CCNNGG 5 cut(s) 223, 342, 534, 732, 1188
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 992
Bse118I RCCGGY 1 cut(s) 915
Bse1I ACTGG 2 cut(s) 282, 436
Bse21I CCTNAGG 1 cut(s) 882
Bse3DI GCAATG 3 cut(s) 910, 951, 1169
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 5 cut(s) 223, 342, 534, 732, 1188
BseGI GGATG 8 cut(s) 167, 305, 427, 495, 835, 953, 1018, 1173
BseLI CCNNNNNNNGG 2 cut(s) 83, 992
BseMI GCAATG 3 cut(s) 910, 951, 1169
BseMII CTCAG 6 cut(s) 365, 524, 602, 849, 873, 1143
BseNI ACTGG 2 cut(s) 282, 436
BseRI GAGGAG 1 cut(s) 135
BseXI GCAGC 4 cut(s) 141, 679, 874, 1076
BseYI CCCAGC 1 cut(s) 380
BsgI GTGCAG 1 cut(s) 873
Bsh1236I CGCG 2 cut(s) 195, 234
BshFI GGCC 5 cut(s) 76, 191, 435, 939, 1194
BshNI GGYRCC 1 cut(s) 410
BsiHKAI GWGCWC 2 cut(s) 273, 461
BsiHKCI CYCGRG 1 cut(s) 1133
BsiSI CCGG 2 cut(s) 342, 916
BslFI GGGAC 5 cut(s) 31, 171, 513, 540, 1083
BslI CCNNNNNNNGG 2 cut(s) 83, 992
BsmAI GTCTC 2 cut(s) 566, 1023
BsmFI GGGAC 5 cut(s) 31, 171, 513, 540, 1083
BsmI GAATGC 1 cut(s) 1091
BsnI GGCC 5 cut(s) 76, 191, 435, 939, 1194
Bso31I GGTCTC 1 cut(s) 1023
BsoBI CYCGRG 1 cut(s) 1133
Bsp1286I GDGCHC 2 cut(s) 273, 461
Bsp1407I TGTACA 1 cut(s) 1170
Bsp143I GATC 5 cut(s) 214, 770, 877, 1100, 1115
Bsp19I CCATGG 1 cut(s) 732
BspACI CCGC 7 cut(s) 108, 132, 171, 193, 234, 626, 1113
BspANI GGCC 5 cut(s) 76, 191, 435, 939, 1194
BspCNI CTCAG 6 cut(s) 366, 523, 601, 850, 874, 1144
BspFNI CGCG 2 cut(s) 195, 234
BspLI GGNNCC 6 cut(s) 144, 241, 412, 501, 502, 940
BspMAI CTGCAG 2 cut(s) 131, 786
BspOI GCTAGC 1 cut(s) 747
BspPI GGATC 3 cut(s) 872, 1108, 1123
BspQI GCTCTTC 1 cut(s) 255
BspT107I GGYRCC 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 1023
BsrBI CCGCTC 1 cut(s) 626
BsrDI GCAATG 3 cut(s) 910, 951, 1169
BsrFI RCCGGY 1 cut(s) 915
BsrGI TGTACA 1 cut(s) 1170
BsrI ACTGG 2 cut(s) 282, 436
BssAI RCCGGY 1 cut(s) 915
BssECI CCNNGG 5 cut(s) 223, 342, 534, 732, 1188
BssMI GATC 5 cut(s) 214, 770, 877, 1100, 1115
BssT1I CCWWGG 3 cut(s) 534, 732, 1188
Bst2UI CCWGG 1 cut(s) 225
Bst6I CTCTTC 4 cut(s) 21, 255, 783, 1206
BstAPI GCANNNNNTGC 1 cut(s) 1013
BstAUI TGTACA 1 cut(s) 1170
BstC8I GCNNGC 3 cut(s) 193, 230, 745
BstDEI CTNAG 7 cut(s) 374, 510, 588, 848, 858, 882, 1152
BstDSI CCRYGG 1 cut(s) 732
BstEII GGTNACC 1 cut(s) 1144
BstF5I GGATG 8 cut(s) 167, 305, 427, 495, 835, 953, 1018, 1173
BstFNI CGCG 2 cut(s) 195, 234
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 5 cut(s) 217, 773, 880, 1103, 1118
BstMAI GTCTC 2 cut(s) 566, 1023
BstMBI GATC 5 cut(s) 214, 770, 877, 1100, 1115
BstMWI GCNNNNNNNGC 3 cut(s) 135, 1013, 1022
BstNI CCWGG 1 cut(s) 225
BstPI GGTNACC 1 cut(s) 1144
BstSCI CCNGG 2 cut(s) 223, 341
BstSFI CTRYAG 3 cut(s) 127, 161, 782
BstUI CGCG 2 cut(s) 195, 234
BstV1I GCAGC 4 cut(s) 141, 679, 874, 1076
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 1115
BstXI CCANNNNNNTGG 2 cut(s) 739, 1009
BstYI RGATCY 1 cut(s) 1115
Bsu36I CCTNAGG 1 cut(s) 882
BsuRI GGCC 5 cut(s) 76, 191, 435, 939, 1194
BtgI CCRYGG 1 cut(s) 732
BtsCI GGATG 8 cut(s) 167, 305, 427, 495, 835, 953, 1018, 1173
BtsI GCAGTG 4 cut(s) 355, 930, 1014, 1114
BtsIMutI CAGTG 6 cut(s) 355, 518, 855, 930, 1014, 1114
Cac8I GCNNGC 3 cut(s) 193, 230, 745
CaiI CAGNNNCTG 1 cut(s) 790
CfoI GCGC 1 cut(s) 234
Cfr10I RCCGGY 1 cut(s) 915
Cfr13I GGNCC 6 cut(s) 74, 190, 204, 500, 938, 1192
CseI GACGC 2 cut(s) 184, 542
Csp6I GTAC 2 cut(s) 546, 1171
CviAII CATG 6 cut(s) 185, 328, 454, 464, 733, 1104
CviQI GTAC 2 cut(s) 546, 1171
DdeI CTNAG 7 cut(s) 374, 510, 588, 848, 858, 882, 1152
DpnI GATC 5 cut(s) 216, 772, 879, 1102, 1117
DpnII GATC 5 cut(s) 214, 770, 877, 1100, 1115
Eam1104I CTCTTC 4 cut(s) 21, 255, 783, 1206
EarI CTCTTC 4 cut(s) 21, 255, 783, 1206
EciI GGCGGA 2 cut(s) 160, 1128
Ecl136II GAGCTC 1 cut(s) 459
Eco130I CCWWGG 3 cut(s) 534, 732, 1188
Eco24I GRGCYC 1 cut(s) 461
Eco31I GGTCTC 1 cut(s) 1023
Eco32I GATATC 1 cut(s) 1210
Eco47I GGWCC 2 cut(s) 204, 500
Eco53kI GAGCTC 1 cut(s) 459
Eco81I CCTNAGG 1 cut(s) 882
Eco88I CYCGRG 1 cut(s) 1133
Eco91I GGTNACC 1 cut(s) 1144
EcoICRI GAGCTC 1 cut(s) 459
EcoO109I RGGNCCY 3 cut(s) 500, 938, 1192
EcoO65I GGTNACC 1 cut(s) 1144
EcoRII CCWGG 1 cut(s) 223
EcoRV GATATC 1 cut(s) 1210
EcoT14I CCWWGG 3 cut(s) 534, 732, 1188
EcoT22I ATGCAT 1 cut(s) 953
EcoT38I GRGCYC 1 cut(s) 461
ErhI CCWWGG 3 cut(s) 534, 732, 1188
FaeI CATG 6 cut(s) 188, 331, 457, 467, 736, 1107
FalI AAGNNNNNCTT 2 cut(s) 705, 737
FaqI GGGAC 5 cut(s) 31, 171, 513, 540, 1083
FatI CATG 6 cut(s) 184, 327, 453, 463, 732, 1103
FauI CCCGC 1 cut(s) 200
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 4 cut(s) 130, 693, 888, 1090
FokI GGATG 8 cut(s) 154, 292, 434, 502, 842, 960, 1025, 1180
FriOI GRGCYC 1 cut(s) 461
Fsp4HI GCNGC 4 cut(s) 130, 693, 888, 1090
FspBI CTAG 1 cut(s) 744
GlaI GCGC 1 cut(s) 233
GluI GCNGC 4 cut(s) 130, 693, 888, 1090
GsaI CCCAGC 1 cut(s) 384
GsuI CTGGAG 2 cut(s) 812, 1100
HaeIII GGCC 5 cut(s) 76, 191, 435, 939, 1194
HapII CCGG 2 cut(s) 342, 916
HgaI GACGC 2 cut(s) 184, 542
HhaI GCGC 1 cut(s) 234
Hin1II CATG 6 cut(s) 188, 331, 457, 467, 736, 1107
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 4 cut(s) 317, 390, 685, 794
HpaII CCGG 2 cut(s) 342, 916
HphI GGTGA 9 cut(s) 97, 293, 298, 575, 765, 875, 940, 1075, 1138
Hpy166II GTNNAC 2 cut(s) 181, 866
Hpy188I TCNGA 3 cut(s) 368, 389, 591
Hpy188III TCNNGA 4 cut(s) 480, 881, 1079, 1205
Hpy8I GTNNAC 2 cut(s) 181, 866
Hpy99I CGWCG 2 cut(s) 182, 200
HpyAV CCTTC 1 cut(s) 1060
HpyCH4IV ACGT 1 cut(s) 932
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 1013, 1022
HpyF3I CTNAG 7 cut(s) 374, 510, 588, 848, 858, 882, 1152
HpySE526I ACGT 1 cut(s) 932
Hsp92II CATG 6 cut(s) 188, 331, 457, 467, 736, 1107
HspAI GCGC 1 cut(s) 232
KflI GGGWCCC 1 cut(s) 500
Kzo9I GATC 5 cut(s) 214, 770, 877, 1100, 1115
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 4 cut(s) 86, 148, 245, 1022
Lsp1109I GCAGC 4 cut(s) 141, 679, 874, 1076
LweI GCATC 6 cut(s) 176, 520, 842, 938, 1003, 1170
MaeI CTAG 1 cut(s) 744
MaeII ACGT 1 cut(s) 932
MaeIII GTNAC 4 cut(s) 103, 449, 928, 1144
MalI GATC 5 cut(s) 216, 772, 879, 1102, 1117
MbiI CCGCTC 1 cut(s) 626
MboI GATC 5 cut(s) 214, 770, 877, 1100, 1115
MboII GAAGA 5 cut(s) 38, 272, 301, 770, 1193
MfeI CAATTG 1 cut(s) 255
MflI RGATCY 1 cut(s) 1115
MhlI GDGCHC 2 cut(s) 273, 461
MluCI AATT 7 cut(s) 50, 255, 405, 592, 673, 896, 1156
MlyI GAGTC 1 cut(s) 803
MmeI TCCRAC 1 cut(s) 141
Mph1103I ATGCAT 1 cut(s) 953
MroXI GAANNNNTTC 2 cut(s) 14, 684
MseI TTAA 1 cut(s) 633
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 2 cut(s) 342, 916
MspR9I CCNGG 2 cut(s) 225, 343
MunI CAATTG 1 cut(s) 255
Mva1269I GAATGC 1 cut(s) 1091
MvaI CCWGG 1 cut(s) 225
MvnI CGCG 2 cut(s) 195, 234
MwoI GCNNNNNNNGC 3 cut(s) 135, 1013, 1022
NciI CCSGG 1 cut(s) 343
NcoI CCATGG 1 cut(s) 732
NdeII GATC 5 cut(s) 214, 770, 877, 1100, 1115
NheI GCTAGC 1 cut(s) 743
NlaIII CATG 6 cut(s) 188, 331, 457, 467, 736, 1107
NlaIV GGNNCC 6 cut(s) 144, 241, 412, 501, 502, 940
NmuCI GTSAC 3 cut(s) 103, 928, 1144
NsiI ATGCAT 1 cut(s) 953
PciSI GCTCTTC 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 550
PctI GAATGC 1 cut(s) 1091
PdmI GAANNNNTTC 2 cut(s) 14, 684
PfeI GAWTC 3 cut(s) 317, 390, 685
PkrI GCNGC 4 cut(s) 131, 694, 889, 1091
PleI GAGTC 1 cut(s) 802
PpsI GAGTC 1 cut(s) 802
PpuMI RGGWCCY 1 cut(s) 500
Psp124BI GAGCTC 1 cut(s) 461
Psp5II RGGWCCY 1 cut(s) 500
Psp6I CCWGG 1 cut(s) 223
PspEI GGTNACC 1 cut(s) 1144
PspFI CCCAGC 1 cut(s) 380
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 6 cut(s) 144, 241, 412, 501, 502, 940
PspPI GGNCC 6 cut(s) 74, 190, 204, 500, 938, 1192
PspPPI RGGWCCY 1 cut(s) 500
PstI CTGCAG 2 cut(s) 131, 786
PstNI CAGNNNCTG 1 cut(s) 790
PsuI RGATCY 1 cut(s) 1115
RsaI GTAC 2 cut(s) 547, 1172
RsaNI GTAC 2 cut(s) 546, 1171
SacI GAGCTC 1 cut(s) 461
SalI GTCGAC 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 1 cut(s) 633
SatI GCNGC 4 cut(s) 130, 693, 888, 1090
Sau3AI GATC 5 cut(s) 214, 770, 877, 1100, 1115
Sau96I GGNCC 6 cut(s) 74, 190, 204, 500, 938, 1192
SchI GAGTC 1 cut(s) 803
ScrFI CCNGG 2 cut(s) 225, 343
SduI GDGCHC 2 cut(s) 273, 461
SfaNI GCATC 6 cut(s) 176, 520, 842, 938, 1003, 1170
SfcI CTRYAG 3 cut(s) 127, 161, 782
SinI GGWCC 2 cut(s) 204, 500
SmlI CTYRAG 3 cut(s) 266, 478, 565
SmoI CTYRAG 3 cut(s) 266, 478, 565
Sse9I AATT 7 cut(s) 50, 255, 405, 592, 673, 896, 1156
SsiI CCGC 7 cut(s) 108, 132, 171, 193, 234, 626, 1113
SspMI CTAG 1 cut(s) 744
SstI GAGCTC 1 cut(s) 461
StyD4I CCNGG 2 cut(s) 223, 341
StyI CCWWGG 3 cut(s) 534, 732, 1188
TaiI ACGT 1 cut(s) 935
TaqI TCGA 4 cut(s) 8, 180, 198, 213
TasI AATT 7 cut(s) 50, 255, 405, 592, 673, 896, 1156
TatI WGTACW 1 cut(s) 1170
TfiI GAWTC 3 cut(s) 317, 390, 685
Tru1I TTAA 1 cut(s) 633
Tru9I TTAA 1 cut(s) 633
TscAI CASTG 6 cut(s) 355, 518, 862, 930, 1014, 1114
TseFI GTSAC 3 cut(s) 103, 928, 1144
TseI GCWGC 4 cut(s) 129, 692, 887, 1089
Tsp45I GTSAC 3 cut(s) 103, 928, 1144
TspDTI ATGAA 3 cut(s) 344, 506, 846
TspGWI ACGGA 2 cut(s) 715, 978
TspRI CASTG 6 cut(s) 355, 518, 862, 930, 1014, 1114
VpaK11BI GGWCC 2 cut(s) 204, 500
XapI RAATTY 1 cut(s) 896
XmiI GTMKAC 1 cut(s) 180
XmnI GAANNNNTTC 2 cut(s) 14, 684
XspI CTAG 1 cut(s) 744
Zsp2I ATGCAT 1 cut(s) 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.