MD08G1031400.v1.1

Cytosolic purine 5'-nucleotidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003145
Physical Location & Seq
Reverse (-)
2246772 .. 2251574
4803 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1031400.v1.1.491

Sequence Viewer

Length: 1926 bp
ATGTTACTTGCTCCGATTCCTTTTCCTAATCCCATTGGCATCGGAATCTCCCAGATTATTCCCCTCGAATCCGATTCCCTCTTATCATCCCTCCCATTCCCTCCTCTTCCCAAATGCCTATTTAAAACCACCATGCGTGTTCCCAAGAAACCCGTTTCCTTCTGCCTCTCTCTGTTTTCTCCCGACCTTTCCGCTGCCGCCCCTTCCTCTCTCTCTCCCCGCCTCCGCTCTCGCTCTCCCGGTAAACGAAAAGGCATTCACACGATAGGAGGTTATCAATTGCATGCCAGCTATTCGATGGAAAGGGGCCACGAGACTGCTGTAGGAGGTGACAAGCCATCACATTTTGCAACTATGGGCTCGACGGATGGTGGTCAGAAACCATGTGTGTGGTCATCTCCCCAAGGAGGATGTAAAATAGACTTAAGAAAGCAGATTTTCTGCAACAGATCGTTGAATATGAAGAATATTGTCGCGGTGGGATTTGATATGGATTACACATTGGCACAGTACAAGCCGGAAACCTTTGAGTCTCTTGCATACGATGGAACTATCCGAAAGTTGGTGTATGATCTAGGATACCCTCGTGAGTTGCTGAATTGGTCCTTCGATTGGACATATATGGTCAGAGGGTTGGTTCTGGATAAGAAGAGAGGCAACATCCTGAAGATGGATCGACATAAGTATGTGAAAGTTGCTTACCATGGATTCAAAGAGTTATCTAAGGAAGATAAAGTTAACACCTATGGAAATACATTAATACGGGATTCCTTTGACGAGCCAGATTATATTATCATTGATACCCTCTTTGCTCTAGCTGAAGCTTACATGTTTGCCCAACTGGTTGACTTCAAAGACAAAAATCCAGGAAAAATTCCAGAGGGTGTTGATTATGCTGTTATGTACAAGGATGTTCGAGCTGCTGTTGATTTGTGCCACCGTGATGGAACTTTAAAGCAAATGGTGGCAAATGACCCTCAGCGGTATATTAATGAAGATACCTCCATAGTTCCTATGCTCAAAATGCTGAGAGATTCTGGCCGTGCTACATTCCTGGTGACAAACAGTTTATGGGACTACACAAACATTGTGATGAATTTCCTCTGCGAGTCACGTACAGTGGATGGTAGTAGAAAATGCAATTTCGACTGGCTTAAATACTTTGATGTTGTCATCACTGGCAGTGCAAAGCCAAGCTTCTTTCATGATGGTAACCGTGCTAATCTGTTTGAGGTAGAACTCGAGTCAGGGATGCTAATCAATACTGATAATGGTTCTCCTATGGCTCAGGTTGGAAGTTCTTCACCTGCACCTACGTTATCGTTGAAGGGACAGAAGAAGGCTTGTAGAGTTTTCCAGGGAGGCAGCGTTGGCCATCTTCATAAACTGCTTTCAATTGAGTCAAGTTCACAGGTTCTTTATGTTGGTGACCATATATATGGAGATATTTTGCGCAGCAAAAAGGTTCTTGGGTGGAGAACAATGCTTGTCGTTCCAGAGCTAGAGAGGGAGGTTGAACTCCTATGGGAATTGAGAGATACCCGCAAGCAACTTCGATTGTTGAGGAATGAACGCGATCTGATTGAAGACAAAATACATCATCTAAAGTGGTCTCTCAAATATGATGATTTTAGTACCGTGGAGAAGCAAACATTATCTTCTGAATGCAATGCTTTGGAGTTTCAAAGAGATCAAGTGCGTTTCGCCCATCAACAGGCTCAACGTGAATGTCACCAAATGTTTCATAAGATCTGGGGGCAACTCATGAAGACTGGCTATCAGAACTCCCGCTTTGCCCATCAGGTCGAGAGATTTGCCTGCCTTTATACTAGCCAAGTTTCCAACTTGAGTCTCTACTCTCCCGACAAATACTACAGGCCGAGCGAAGACTACATGCCCCATGAATTCGATATTCTTCCCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

642

Amino Acids

73.15

Weight (kDa)

8.59

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 146 - 636 1.1e-176 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1315
Acc16I TGCGCA 1 cut(s) 1454
Acc36I ACCTGC 1 cut(s) 1315
AccBSI CCGCTC 1 cut(s) 228
AccII CGCG 2 cut(s) 476, 1575
AciI CCGC 8 cut(s) 192, 198, 220, 226, 476, 982, 1543, 1789
AclWI GGATC 1 cut(s) 681
AcoI YGGCCR 2 cut(s) 1039, 1372
AcsI RAATTY 3 cut(s) 873, 1096, 1904
AcuI CTGAAG 2 cut(s) 686, 840
AfaI GTAC 4 cut(s) 512, 905, 1117, 1636
AfiI CCNNNNNNNGG 5 cut(s) 407, 562, 612, 670, 1714
AflII CTTAAG 1 cut(s) 424
AflIII ACRYGT 1 cut(s) 828
AgsI TTSAA 8 cut(s) 457, 712, 853, 1327, 1395, 1517, 1586, 1685
AjnI CCWGG 3 cut(s) 865, 1053, 1356
AluBI AGCT 6 cut(s) 291, 818, 824, 920, 1197, 1501
AluI AGCT 6 cut(s) 291, 818, 824, 920, 1197, 1501
Alw26I GTCTC 4 cut(s) 308, 537, 1617, 1856
AlwI GGATC 1 cut(s) 681
Ama87I CYCGRG 1 cut(s) 1241
AoxI GGCC 4 cut(s) 307, 1039, 1372, 1877
ApeKI GCWGC 4 cut(s) 194, 920, 1365, 1455
ApoI RAATTY 3 cut(s) 873, 1096, 1904
AseI ATTAAT 2 cut(s) 758, 990
Asp700I GAANNNNTTC 1 cut(s) 1300
AspLEI GCGC 1 cut(s) 1455
AspS9I GGNCC 2 cut(s) 307, 603
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 5 cut(s) 341, 1069, 1296, 1439, 1724
AvaI CYCGRG 1 cut(s) 1241
AvaII GGWCC 1 cut(s) 603
BaeI ACNNNNGTAYC 2 cut(s) 571, 604
BalI TGGCCA 1 cut(s) 1374
BanII GRGCYC 1 cut(s) 362
BarI GAAGNNNNNNTAC 2 cut(s) 1578, 1610
BauI CACGAG 2 cut(s) 311, 585
BbsI GAAGAC 3 cut(s) 1593, 1775, 1893
BbvCI CCTCAGC 1 cut(s) 978
BbvI GCAGC 4 cut(s) 181, 907, 1377, 1467
BceAI ACGGC 1 cut(s) 1026
BcgI CGANNNNNNTGC 2 cut(s) 1796, 1830
BciT130I CCWGG 3 cut(s) 867, 1055, 1358
BciVI GTATCC 1 cut(s) 572
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 4 cut(s) 308, 537, 1617, 1856
BfaI CTAG 4 cut(s) 575, 815, 1502, 1830
BfmI CTRYAG 2 cut(s) 321, 1873
BfrI CTTAAG 1 cut(s) 424
BfuAI ACCTGC 1 cut(s) 1315
BfuI GTATCC 1 cut(s) 572
BglII AGATCT 1 cut(s) 1749
BisI GCNGC 5 cut(s) 195, 198, 921, 1366, 1456
BlsI GCNGC 5 cut(s) 196, 199, 922, 1367, 1457
Bme1390I CCNGG 4 cut(s) 240, 867, 1055, 1358
Bme18I GGWCC 1 cut(s) 603
BmeT110I CYCGRG 1 cut(s) 1241
BmgT120I GGNCC 2 cut(s) 307, 603
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 4 cut(s) 240, 867, 1055, 1358
BmsI GCATC 2 cut(s) 48, 1242
BpiI GAAGAC 3 cut(s) 1593, 1775, 1893
Bpu10I CCTNAGC 2 cut(s) 978, 1287
BpuEI CTTGAG 1 cut(s) 1867
BpuMI CCSGG 1 cut(s) 240
BsaAI YACGTR 1 cut(s) 1115
BsaBI GATNNNNATC 1 cut(s) 1256
BsaI GGTCTC 1 cut(s) 1617
BsaJI CCNNGG 4 cut(s) 403, 703, 1357, 1638
BsaXI ACNNNNNCTCC 2 cut(s) 1353, 1383
Bsc4I CCNNNNNNNGG 5 cut(s) 407, 562, 612, 670, 1714
Bse1I ACTGG 4 cut(s) 846, 1154, 1183, 1777
Bse3DI GCAATG 1 cut(s) 1675
Bse8I GATNNNNATC 1 cut(s) 1256
BseBI CCWGG 3 cut(s) 867, 1055, 1358
BseDI CCNNGG 4 cut(s) 403, 703, 1357, 1638
BseGI GGATG 7 cut(s) 86, 373, 416, 660, 916, 1129, 1257
BseJI GATNNNNATC 1 cut(s) 1256
BseLI CCNNNNNNNGG 5 cut(s) 407, 562, 612, 670, 1714
BseMI GCAATG 1 cut(s) 1675
BseMII CTCAG 3 cut(s) 992, 1019, 1301
BseNI ACTGG 4 cut(s) 846, 1154, 1183, 1777
BseRI GAGGAG 1 cut(s) 93
BseXI GCAGC 4 cut(s) 181, 907, 1377, 1467
BsgI GTGCAG 1 cut(s) 1293
Bsh1236I CGCG 2 cut(s) 476, 1575
BshFI GGCC 4 cut(s) 309, 1041, 1374, 1879
BsiHKCI CYCGRG 1 cut(s) 1241
BsiSI CCGG 2 cut(s) 240, 518
BslFI GGGAC 2 cut(s) 1088, 1344
BslI CCNNNNNNNGG 5 cut(s) 407, 562, 612, 670, 1714
BsmAI GTCTC 4 cut(s) 308, 537, 1617, 1856
BsmFI GGGAC 2 cut(s) 1088, 1344
BsmI GAATGC 2 cut(s) 255, 1670
BsnI GGCC 4 cut(s) 309, 1041, 1374, 1879
Bso31I GGTCTC 1 cut(s) 1617
BsoBI CYCGRG 1 cut(s) 1241
Bsp1286I GDGCHC 1 cut(s) 362
Bsp1407I TGTACA 1 cut(s) 903
Bsp143I GATC 6 cut(s) 449, 571, 673, 1576, 1690, 1749
Bsp19I CCATGG 1 cut(s) 703
BspACI CCGC 8 cut(s) 192, 198, 220, 226, 476, 982, 1543, 1789
BspANI GGCC 4 cut(s) 309, 1041, 1374, 1879
BspCNI CTCAG 3 cut(s) 991, 1020, 1300
BspFNI CGCG 2 cut(s) 476, 1575
BspHI TCATGA 2 cut(s) 1204, 1764
BspLI GGNNCC 1 cut(s) 308
BspMI ACCTGC 1 cut(s) 1315
BspPI GGATC 1 cut(s) 681
BspTI CTTAAG 1 cut(s) 424
BspTNI GGTCTC 1 cut(s) 1617
BsrBI CCGCTC 1 cut(s) 228
BsrDI GCAATG 1 cut(s) 1675
BsrGI TGTACA 1 cut(s) 903
BsrI ACTGG 4 cut(s) 846, 1154, 1183, 1777
BssECI CCNNGG 4 cut(s) 403, 703, 1357, 1638
BssMI GATC 6 cut(s) 449, 571, 673, 1576, 1690, 1749
BssSI CACGAG 2 cut(s) 311, 585
BssT1I CCWWGG 2 cut(s) 403, 703
Bst2BI CACGAG 2 cut(s) 311, 585
Bst2UI CCWGG 3 cut(s) 867, 1055, 1358
Bst4CI ACNGT 6 cut(s) 510, 941, 1067, 1120, 1217, 1639
Bst6I CTCTTC 2 cut(s) 111, 644
BstAFI CTTAAG 1 cut(s) 424
BstAUI TGTACA 1 cut(s) 903
BstBAI YACGTR 1 cut(s) 1115
BstC8I GCNNGC 4 cut(s) 285, 289, 1547, 1819
BstDEI CTNAG 4 cut(s) 723, 978, 1028, 1287
BstDSI CCRYGG 2 cut(s) 703, 1638
BstEII GGTNACC 2 cut(s) 1211, 1427
BstF5I GGATG 7 cut(s) 86, 373, 416, 660, 916, 1129, 1257
BstFNI CGCG 2 cut(s) 476, 1575
BstHHI GCGC 1 cut(s) 1455
BstKTI GATC 6 cut(s) 452, 574, 676, 1579, 1693, 1752
BstMAI GTCTC 4 cut(s) 308, 537, 1617, 1856
BstMBI GATC 6 cut(s) 449, 571, 673, 1576, 1690, 1749
BstMWI GCNNNNNNNGC 2 cut(s) 1024, 1371
BstNI CCWGG 3 cut(s) 867, 1055, 1358
BstNSI RCATGY 3 cut(s) 287, 832, 1897
BstPI GGTNACC 2 cut(s) 1211, 1427
BstSCI CCNGG 4 cut(s) 238, 865, 1053, 1356
BstSFI CTRYAG 2 cut(s) 321, 1873
BstUI CGCG 2 cut(s) 476, 1575
BstV1I GCAGC 4 cut(s) 181, 907, 1377, 1467
BstV2I GAAGAC 3 cut(s) 1593, 1775, 1893
BstX2I RGATCY 1 cut(s) 1749
BstXI CCANNNNNNTGG 3 cut(s) 390, 944, 1439
BstYI RGATCY 1 cut(s) 1749
BsuI GTATCC 1 cut(s) 572
BsuRI GGCC 4 cut(s) 309, 1041, 1374, 1879
BtgI CCRYGG 2 cut(s) 703, 1638
BtsCI GGATG 7 cut(s) 86, 373, 416, 660, 916, 1129, 1257
BtsI GCAGTG 1 cut(s) 1189
BtsIMutI CAGTG 3 cut(s) 1125, 1176, 1189
BveI ACCTGC 1 cut(s) 1315
Cac8I GCNNGC 4 cut(s) 285, 289, 1547, 1819
CciI TCATGA 2 cut(s) 1204, 1764
CfoI GCGC 1 cut(s) 1455
Cfr13I GGNCC 2 cut(s) 307, 603
Csp6I GTAC 4 cut(s) 511, 904, 1116, 1635
CviAII CATG 9 cut(s) 133, 284, 384, 704, 829, 1205, 1765, 1894, 1901
CviQI GTAC 4 cut(s) 511, 904, 1116, 1635
DdeI CTNAG 4 cut(s) 723, 978, 1028, 1287
DpnI GATC 6 cut(s) 451, 573, 675, 1578, 1692, 1751
DpnII GATC 6 cut(s) 449, 571, 673, 1576, 1690, 1749
DraI TTTAAA 2 cut(s) 124, 954
EaeI YGGCCR 2 cut(s) 1039, 1372
Eam1104I CTCTTC 2 cut(s) 111, 644
EarI CTCTTC 2 cut(s) 111, 644
Eco130I CCWWGG 2 cut(s) 403, 703
Eco24I GRGCYC 1 cut(s) 362
Eco31I GGTCTC 1 cut(s) 1617
Eco47I GGWCC 1 cut(s) 603
Eco57I CTGAAG 2 cut(s) 686, 840
Eco88I CYCGRG 1 cut(s) 1241
Eco91I GGTNACC 2 cut(s) 1211, 1427
EcoO65I GGTNACC 2 cut(s) 1211, 1427
EcoRI GAATTC 1 cut(s) 1904
EcoRII CCWGG 3 cut(s) 865, 1053, 1356
EcoT14I CCWWGG 2 cut(s) 403, 703
EcoT38I GRGCYC 1 cut(s) 362
ErhI CCWWGG 2 cut(s) 403, 703
FaeI CATG 9 cut(s) 136, 287, 387, 707, 832, 1208, 1768, 1897, 1904
FalI AAGNNNNNCTT 2 cut(s) 1181, 1213
FaqI GGGAC 2 cut(s) 1088, 1344
FatI CATG 9 cut(s) 132, 283, 383, 703, 828, 1204, 1764, 1893, 1900
FauI CCCGC 3 cut(s) 227, 1550, 1796
Fnu4HI GCNGC 5 cut(s) 195, 198, 921, 1366, 1456
FokI GGATG 7 cut(s) 73, 380, 423, 647, 923, 1136, 1264
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 5 cut(s) 195, 198, 921, 1366, 1456
FspBI CTAG 4 cut(s) 575, 815, 1502, 1830
FspI TGCGCA 1 cut(s) 1454
GlaI GCGC 1 cut(s) 1454
GluI GCNGC 5 cut(s) 195, 198, 921, 1366, 1456
HaeIII GGCC 4 cut(s) 309, 1041, 1374, 1879
HapII CCGG 2 cut(s) 240, 518
HhaI GCGC 1 cut(s) 1455
Hin1II CATG 9 cut(s) 136, 287, 387, 707, 832, 1208, 1768, 1897, 1904
Hin6I GCGC 1 cut(s) 1453
HinP1I GCGC 1 cut(s) 1453
HincII GTYRAC 2 cut(s) 739, 847
HindII GTYRAC 2 cut(s) 739, 847
HindIII AAGCTT 2 cut(s) 822, 1195
HpaI GTTAAC 1 cut(s) 739
HpaII CCGG 2 cut(s) 240, 518
HphI GGTGA 5 cut(s) 341, 1069, 1296, 1439, 1724
Hpy166II GTNNAC 4 cut(s) 245, 739, 847, 1409
Hpy188I TCNGA 9 cut(s) 15, 44, 73, 378, 557, 629, 1581, 1663, 1782
Hpy8I GTNNAC 4 cut(s) 245, 739, 847, 1409
Hpy99I CGWCG 1 cut(s) 367
HpyAV CCTTC 5 cut(s) 169, 213, 616, 1321, 1333
HpyCH4III ACNGT 6 cut(s) 510, 941, 1067, 1120, 1217, 1639
HpyCH4IV ACGT 3 cut(s) 1114, 1316, 1723
HpyCH4V TGCA 8 cut(s) 283, 350, 444, 539, 1140, 1187, 1310, 1668
HpyF10VI GCNNNNNNNGC 2 cut(s) 1024, 1371
HpyF3I CTNAG 4 cut(s) 723, 978, 1028, 1287
HpySE526I ACGT 3 cut(s) 1114, 1316, 1723
Hsp92II CATG 9 cut(s) 136, 287, 387, 707, 832, 1208, 1768, 1897, 1904
HspAI GCGC 1 cut(s) 1453
KspAI GTTAAC 1 cut(s) 739
Kzo9I GATC 6 cut(s) 449, 571, 673, 1576, 1690, 1749
LmnI GCTCC 1 cut(s) 16
Lsp1109I GCAGC 4 cut(s) 181, 907, 1377, 1467
LweI GCATC 2 cut(s) 48, 1242
MaeI CTAG 4 cut(s) 575, 815, 1502, 1830
MaeII ACGT 3 cut(s) 1114, 1316, 1723
MaeIII GTNAC 7 cut(s) 3, 329, 1057, 1110, 1211, 1427, 1730
MalI GATC 6 cut(s) 451, 573, 675, 1578, 1692, 1751
MbiI CCGCTC 1 cut(s) 228
MboI GATC 6 cut(s) 449, 571, 673, 1576, 1690, 1749
MfeI CAATTG 2 cut(s) 278, 1395
MflI RGATCY 1 cut(s) 1749
MhlI GDGCHC 1 cut(s) 362
MlsI TGGCCA 1 cut(s) 1374
MluCI AATT 8 cut(s) 278, 598, 873, 1096, 1141, 1395, 1529, 1904
MluNI TGGCCA 1 cut(s) 1374
MlyI GAGTC 5 cut(s) 539, 1118, 1253, 1409, 1858
MmeI TCCRAC 2 cut(s) 1273, 1866
Mox20I TGGCCA 1 cut(s) 1374
MroXI GAANNNNTTC 1 cut(s) 1300
MscI TGGCCA 1 cut(s) 1374
MseI TTAA 7 cut(s) 123, 425, 738, 758, 953, 990, 1155
MslI CAYNNNNRTG 5 cut(s) 388, 684, 942, 1091, 1437
Msp20I TGGCCA 1 cut(s) 1374
MspA1I CMGCKG 2 cut(s) 194, 982
MspCI CTTAAG 1 cut(s) 424
MspI CCGG 2 cut(s) 240, 518
MspR9I CCNGG 4 cut(s) 240, 867, 1055, 1358
MunI CAATTG 2 cut(s) 278, 1395
Mva1269I GAATGC 2 cut(s) 255, 1670
MvaI CCWGG 3 cut(s) 867, 1055, 1358
MvnI CGCG 2 cut(s) 476, 1575
MwoI GCNNNNNNNGC 2 cut(s) 1024, 1371
NciI CCSGG 1 cut(s) 240
NcoI CCATGG 1 cut(s) 703
NdeII GATC 6 cut(s) 449, 571, 673, 1576, 1690, 1749
NlaIII CATG 9 cut(s) 136, 287, 387, 707, 832, 1208, 1768, 1897, 1904
NlaIV GGNNCC 1 cut(s) 308
NmeAIII GCCGAG 1 cut(s) 1905
NmuCI GTSAC 5 cut(s) 329, 1057, 1110, 1427, 1730
NsbI TGCGCA 1 cut(s) 1454
NspI RCATGY 3 cut(s) 287, 832, 1897
PaeI GCATGC 1 cut(s) 287
PaeR7I CTCGAG 1 cut(s) 1241
PagI TCATGA 2 cut(s) 1204, 1764
PaqCI CACCTGC 1 cut(s) 1315
PciI ACATGT 1 cut(s) 828
PctI GAATGC 2 cut(s) 255, 1670
PdmI GAANNNNTTC 1 cut(s) 1300
PfeI GAWTC 7 cut(s) 16, 45, 68, 74, 708, 767, 1034
PfoI TCCNGGA 1 cut(s) 865
PkrI GCNGC 5 cut(s) 196, 199, 922, 1367, 1457
PleI GAGTC 5 cut(s) 538, 1117, 1252, 1408, 1857
PpsI GAGTC 5 cut(s) 538, 1117, 1252, 1408, 1857
Ppu21I YACGTR 1 cut(s) 1115
PscI ACATGT 1 cut(s) 828
PshBI ATTAAT 2 cut(s) 758, 990
Psp6I CCWGG 3 cut(s) 865, 1053, 1356
PspEI GGTNACC 2 cut(s) 1211, 1427
PspGI CCWGG 3 cut(s) 865, 1053, 1356
PspN4I GGNNCC 1 cut(s) 308
PspPI GGNCC 2 cut(s) 307, 603
PspXI VCTCGAGB 1 cut(s) 1241
PsuI RGATCY 1 cut(s) 1749
RsaI GTAC 4 cut(s) 512, 905, 1117, 1636
RsaNI GTAC 4 cut(s) 511, 904, 1116, 1635
RseI CAYNNNNRTG 5 cut(s) 388, 684, 942, 1091, 1437
SaqAI TTAA 7 cut(s) 123, 425, 738, 758, 953, 990, 1155
SatI GCNGC 5 cut(s) 195, 198, 921, 1366, 1456
Sau3AI GATC 6 cut(s) 449, 571, 673, 1576, 1690, 1749
Sau96I GGNCC 2 cut(s) 307, 603
SchI GAGTC 5 cut(s) 539, 1118, 1253, 1409, 1858
ScrFI CCNGG 4 cut(s) 240, 867, 1055, 1358
SduI GDGCHC 1 cut(s) 362
SfaNI GCATC 2 cut(s) 48, 1242
SfcI CTRYAG 2 cut(s) 321, 1873
Sfr274I CTCGAG 1 cut(s) 1241
SinI GGWCC 1 cut(s) 603
SlaI CTCGAG 1 cut(s) 1241
SmiMI CAYNNNNRTG 5 cut(s) 388, 684, 942, 1091, 1437
SmlI CTYRAG 3 cut(s) 424, 1241, 1846
SmoI CTYRAG 3 cut(s) 424, 1241, 1846
SphI GCATGC 1 cut(s) 287
Sse9I AATT 8 cut(s) 278, 598, 873, 1096, 1141, 1395, 1529, 1904
SsiI CCGC 8 cut(s) 192, 198, 220, 226, 476, 982, 1543, 1789
SspI AATATT 1 cut(s) 469
SspMI CTAG 4 cut(s) 575, 815, 1502, 1830
StyD4I CCNGG 4 cut(s) 238, 865, 1053, 1356
StyI CCWWGG 2 cut(s) 403, 703
TaaI ACNGT 6 cut(s) 510, 941, 1067, 1120, 1217, 1639
TaiI ACGT 3 cut(s) 1117, 1319, 1726
TasI AATT 8 cut(s) 278, 598, 873, 1096, 1141, 1395, 1529, 1904
TatI WGTACW 2 cut(s) 510, 903
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 7 cut(s) 16, 45, 68, 74, 708, 767, 1034
Tru1I TTAA 7 cut(s) 123, 425, 738, 758, 953, 990, 1155
Tru9I TTAA 7 cut(s) 123, 425, 738, 758, 953, 990, 1155
TscAI CASTG 3 cut(s) 1125, 1183, 1189
TseFI GTSAC 5 cut(s) 329, 1057, 1110, 1427, 1730
TseI GCWGC 4 cut(s) 194, 920, 1365, 1455
Tsp45I GTSAC 5 cut(s) 329, 1057, 1110, 1427, 1730
TspDTI ATGAA 9 cut(s) 476, 1008, 1109, 1193, 1370, 1584, 1733, 1781, 1917
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 3 cut(s) 1125, 1183, 1189
Vha464I CTTAAG 1 cut(s) 424
VpaK11BI GGWCC 1 cut(s) 603
VspI ATTAAT 2 cut(s) 758, 990
XapI RAATTY 3 cut(s) 873, 1096, 1904
XceI RCATGY 3 cut(s) 287, 832, 1897
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 1241
XmnI GAANNNNTTC 1 cut(s) 1300
XspI CTAG 4 cut(s) 575, 815, 1502, 1830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.