Rorug06G0300800

Cytosolic purine 5'-nucleotidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003145
Physical Location & Seq
Forward (+)
45262558 .. 45264217
1660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0300800.1

Sequence Viewer

Length: 387 bp
ATGGGAAGAAGGAAACGTGGGTTTGAAGAAGAAGAGGGTGTGCTTCAAATGTCAAACATTCTCTGTGCTATTATTACGTTTTTGCCCCTATACTATTGGGCCGGTTTCAGATCCGATTGGAGGGGCGAACCCTCCTCCGCGCCTACATTTGAGCTTTTCAGGGGTTATGAGTTTGATGATGGAATTGTTGGGCGGATTATTGGGTTCTACATCAACGAGAATGGTGATAAAGTCTACACCACCAAGAAAGAATCACCACTGGGGCTTGCTACGCAATCTGCCCACCCTGCCCGCTTCTCCCCTGATGACAAGTATTCAAGGCAAAGATATCTTCTGAAGAAGCGCTTTGGATTGTTGCCAACCCAGCAGTCTCCTCTCAAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

128

Amino Acids

14.83

Weight (kDa)

9.24

Isoelectric Point (pI)

64.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop10p PF04135 69 - 116 3.1e-19 Nucleolar RNA-binding protein, Nop10p family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 234
AccII CGCG 1 cut(s) 140
AciI CCGC 3 cut(s) 138, 193, 292
AclWI GGATC 1 cut(s) 105
AcuI CTGAAG 1 cut(s) 356
AfeI AGCGCT 1 cut(s) 344
AfiI CCNNNNNNNGG 1 cut(s) 120
AgsI TTSAA 3 cut(s) 26, 47, 318
AluBI AGCT 1 cut(s) 154
AluI AGCT 1 cut(s) 154
Alw26I GTCTC 1 cut(s) 375
AlwI GGATC 1 cut(s) 105
Aor51HI AGCGCT 1 cut(s) 344
AoxI GGCC 1 cut(s) 99
AspLEI GCGC 2 cut(s) 142, 345
AspS9I GGNCC 1 cut(s) 99
AsuHPI GGTGA 2 cut(s) 236, 246
BccI CCATC 1 cut(s) 173
BcoDI GTCTC 1 cut(s) 375
BfoI RGCGCY 1 cut(s) 346
BmgT120I GGNCC 1 cut(s) 99
BmrI ACTGGG 1 cut(s) 269
BmuI ACTGGG 1 cut(s) 269
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 264
BseLI CCNNNNNNNGG 1 cut(s) 120
BseNI ACTGG 1 cut(s) 264
BseRI GAGGAG 2 cut(s) 124, 363
BseYI CCCAGC 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 1 cut(s) 101
BsiSI CCGG 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 120
BsmAI GTCTC 1 cut(s) 375
BsnI GGCC 1 cut(s) 101
Bsp143I GATC 1 cut(s) 110
BspACI CCGC 3 cut(s) 138, 193, 292
BspANI GGCC 1 cut(s) 101
BspFNI CGCG 1 cut(s) 140
BspPI GGATC 1 cut(s) 105
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 1 cut(s) 264
BssAI RCCGGY 1 cut(s) 101
BssMI GATC 1 cut(s) 110
Bst6I CTCTTC 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 267, 292
BstFNI CGCG 1 cut(s) 140
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 2 cut(s) 142, 345
BstKTI GATC 1 cut(s) 113
BstMAI GTCTC 1 cut(s) 375
BstMBI GATC 1 cut(s) 110
BstMWI GCNNNNNNNGC 3 cut(s) 271, 287, 364
BstUI CGCG 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 110
BstYI RGATCY 1 cut(s) 110
BsuRI GGCC 1 cut(s) 101
BtsIMutI CAGTG 1 cut(s) 257
Cac8I GCNNGC 2 cut(s) 267, 292
CfoI GCGC 2 cut(s) 142, 345
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 1 cut(s) 99
CviJI RGCY 3 cut(s) 101, 154, 265
CviKI_1 RGCY 3 cut(s) 101, 154, 265
DpnI GATC 1 cut(s) 112
DpnII GATC 1 cut(s) 110
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
EciI GGCGGA 1 cut(s) 208
Eco32I GATATC 1 cut(s) 329
Eco47III AGCGCT 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 356
EcoRV GATATC 1 cut(s) 329
FaiI YATR 2 cut(s) 91, 168
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FauI CCCGC 1 cut(s) 299
FblI GTMKAC 1 cut(s) 234
GlaI GCGC 2 cut(s) 141, 344
GsaI CCCAGC 1 cut(s) 367
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 1 cut(s) 101
HapII CCGG 1 cut(s) 102
HhaI GCGC 2 cut(s) 142, 345
Hin6I GCGC 2 cut(s) 140, 343
HinP1I GCGC 2 cut(s) 140, 343
HinfI GANTC 1 cut(s) 251
HpaII CCGG 1 cut(s) 102
HphI GGTGA 2 cut(s) 236, 246
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 3 cut(s) 110, 115, 336
Hpy8I GTNNAC 1 cut(s) 235
HpyAV CCTTC 1 cut(s) 3
HpyCH4IV ACGT 2 cut(s) 16, 77
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 287, 364
HpySE526I ACGT 2 cut(s) 16, 77
HspAI GCGC 2 cut(s) 140, 343
Kzo9I GATC 1 cut(s) 110
LpnPI CCDG 6 cut(s) 115, 145, 245, 300, 315, 377
MaeII ACGT 2 cut(s) 16, 77
MalI GATC 1 cut(s) 112
MboI GATC 1 cut(s) 110
MboII GAAGA 6 cut(s) 18, 38, 41, 44, 323, 349
MflI RGATCY 1 cut(s) 110
MluCI AATT 1 cut(s) 183
MnlI CCTC 5 cut(s) 28, 114, 142, 145, 384
MspI CCGG 1 cut(s) 102
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 3 cut(s) 271, 287, 364
NdeII GATC 1 cut(s) 110
PfeI GAWTC 1 cut(s) 251
PspFI CCCAGC 1 cut(s) 363
PspPI GGNCC 1 cut(s) 99
PsuI RGATCY 1 cut(s) 110
Sau3AI GATC 1 cut(s) 110
Sau96I GGNCC 1 cut(s) 99
SetI ASST 3 cut(s) 19, 80, 156
Sse9I AATT 1 cut(s) 183
SsiI CCGC 3 cut(s) 138, 193, 292
TaiI ACGT 2 cut(s) 19, 80
TasI AATT 1 cut(s) 183
TfiI GAWTC 1 cut(s) 251
TscAI CASTG 1 cut(s) 264
TspRI CASTG 1 cut(s) 264
XmiI GTMKAC 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.