MD08G1036300.v1.1

Leishmanolysin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010930
Physical Location & Seq
Forward (+)
2670719 .. 2677289
6571 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1036300.v1.1.491

Sequence Viewer

Length: 2445 bp
ATGGAGGTGATTATGCGGCGTCGTCCATGTCCCTCTCTCAGATTCGCCGTCGTTGTCCTCAAGGTTATTTTGCTATTTTTATGGTTGGAAGCTGGTAATGCAACATTCCAAGAAAGCACAGTGCAAGGGCAAAGCCCAGAATTGTTATCCGATAACATTGTTTCACATTCCTGCATTCATGACCAGATAATCAAACAGCGGAGGAGACCTGGTCGCAAAGTGTACACTGTTACCACGCAGGTGTACGAGGGGTCTGGTGTATCAAAACCCCTTCACCAAAAGGGTAGGGCATTACTTGGAATTTCCAAGTACTCTGTACAGCAGAAGGATATTAAAAGGCCTATTAGAATATATTTAAATTATGATGCTGTTGGTCACTCTCCTGATAGAGATTGTCGAAATGTTGGCGATGTTGTGAAGCTTGGGGAGCCACCTGTGATTTCGATTCCTGGCACTCCTTCTTGTAATCCTCATGGTGATCCTCCAATTTCTGGTGACTGCTGGTATAACTGCACCTTGGATGATATAGCTGGGAAGGACAAAAGGCAACGCCTTCGCAAGGCTCTAGGGCAGACTGCAGACTGGTTCAAGAGAGCCTTAGCTGTTGAACCTGTGAGGGGGAACTTGCGGTTGAGTGGGTATTCAGCCTGTGGCCAGGATGGAGGTGTACAACTGCCACGTGAATATGTTGAAGAGGGTGTTGCTGAAGCAGACTTGGTTCTTCTGGTGACAACAAGACCAACCACTGGGAACACCCTTGCATGGGCAGTGGCTTGCGAACGTGATCAATGGGGTCGTGCAATTGCAGGACATGTAAATGTTGCTCCTCGCCATCTGACAGCTGAAGCAGAAACTTTACTTTCTGCTACTCTCATACATGAGGTTATGCATGTTCTCGGTTTTGATCCCCATGCATTTGCTCATTTTAGGGATGAAAGGAAAAGAAGGCGTAGTCAGGTTACAGAACAAATTATGGATGAAAAGCTTGGGCGAATGGTGACTCGTGTGTTGCTTCCACGTGTTGTCATGCATTCAAGATATCATTATGCGGCATTCTCTGAGAATTTCACTGGTTTAGAGCTCGAAGATGGTGGAGGACGCGGCACATCAGGGTCACACTGGGAGAAAAGACTTCTAATGAATGAAATTATGACGGGGTCAGTGGATACAAGATCTGTGGTTTCAAAAATGACACTTGCTTTACTAGAAGATAGTGGATGGTACCAGGCCAATTATAGTATGGCAGAGAATCTTGATTGGGGCCGCAACCAAGGAACTGAATTTGTGACTTCCCCTTGCAATCTCTGGAAGGGGGCATATCATTGCAACGCAACCCAATTGTCAGGATGTACATACAACAGAGAAGCTGAAGGTTACTGTCCGATCGTAAGTTACAGTGGAGACCTACCCCAGTGGGCACGCTACTTTCCACAGGCTAACAAAGGTGGTCAGTCCCCACTGGCTGATTATTGCACCTATTTCGTTGCTTACTCTGATGGGTCATGTACTGACACTAACAGCGCACGGCCACCTGACAAAATGTTGGGTGAAGTACGAGGAAGTAACTCTAGGTGTATGGCTTCATCATTGGTACGTACAGGGTTTGTACGGGGTTCGATGACCCAAGGAAATGGATGTTATCAGCACAGATGTGTTAATAATTCATTAGAGATTGCTGTGGATGGTATGTGGAAAGTATGTCCTGAAGCTGGTGGACCCCTTCAGTTCCCAGGATTTAATGGTGAATTGTTATGCCCGGCATACCATGAACTCTGCAGTAAAGGCCTAATTCCTGCAAATGGACAATGTCCAATGTCGTGCAATTTCAATGGAGACTGTGTTGAAGGAAGGTGCCACTGTTTTCTAGGGTTTCATGGTTCTGATTGTAGTAAACGCACCTGCCCGAGCAACTGTAATGGACGTGGAAGCTGCCTTTCTAATGGGCTGTGTGAATGTAATAAAGGGTACACAGGCATTGACTGCTCCACTGCGGTTTGTGATGAGCAATGCAGCCTTCACGGAGGTGTTTGTGATGATGGAGTCTGTGAATTCCGCTGCTCTGACTATGCAGGCTACTCATGCCAGAACAGCACCATGCTCCAATCCAGTCTTAAAGTTTGTAAAGATGTCCTGGAAAACGTCAACTCTGGTGCCGGACAGCACTGTGCACCCAGTGAACCAAGCATATTGCAGCAGCTTGAGGATGTAGTGGTCATGCCCAACTACCATCGTTTGTTTCCTGGTGGCGCCCGGAAGCTTTTTAGCATCTTCGGAACCAGCTACTGTGACACAACCGCGAAGCAGCTTGCTTGCTGGATATCGATTCAAAAGTGCGACAAGGACGGGGACAACAGACTCCGTGTCTGCTATTCGGCCTGTCAATCATACAATTCGGCATGTGGTGCTTCGCTCGATTGCTCGGACCAAACCCTGTTCAGTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000819 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006323 GO:0006325 GO:0006338 GO:0006508 GO:0006807 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007076 GO:0007088 GO:0007098 GO:0007100 GO:0007275 GO:0007276 GO:0007346 GO:0007399 GO:0007417 GO:0007420 GO:0007444 GO:0007548 GO:0008150 GO:0008152 GO:0008233 GO:0008354 GO:0008406 GO:0009888 GO:0009896 GO:0009987 GO:0010564 GO:0010638 GO:0010965 GO:0016043 GO:0016477 GO:0016787 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030071 GO:0030261 GO:0031023 GO:0031331 GO:0032270 GO:0032436 GO:0032501 GO:0032502 GO:0032504 GO:0033043 GO:0033044 GO:0033045 GO:0033047 GO:0035295 GO:0040011 GO:0043170 GO:0044238 GO:0044424 GO:0044464 GO:0044703 GO:0045137 GO:0045732 GO:0045787 GO:0045840 GO:0045842 GO:0045862 GO:0045931 GO:0048285 GO:0048513 GO:0048518 GO:0048522 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0048870 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051247 GO:0051276 GO:0051298 GO:0051299 GO:0051674 GO:0051704 GO:0051726 GO:0051781 GO:0051783 GO:0051785 GO:0051983 GO:0051984 GO:0060322 GO:0060429 GO:0061458 GO:0062033 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0090068 GO:0098813 GO:0140014 GO:0140096 GO:1901564 GO:1901800 GO:1901970 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902099 GO:1902101 GO:1902850 GO:1903047 GO:1903052 GO:1903364 GO:1905818 GO:1905820 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko05140 ko05143 map05140 map05143
Pfam Domains
Protein Families
EGF_2 Peptidase_M8

Protein Analysis

815

Amino Acids

89.51

Weight (kDa)

7.01

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M8 PF01457 169 - 594 1.6e-80 Leishmanolysin
EGF_TEN PF25024 606 - 752 8.9e-12 Teneurin EGF domain
EGF_Teneurin PF23106 634 - 663 9.3e-06 Teneurin-like EGF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42620 AT5G42620
fragaria_vesca FvH4_2g40290 FvH4_2g40290 FvH4_2g40290
malus_domestica MD08G1036300.v1.1
prunus_persica Prupe.1G383600_v2.0.a1
pyrus_communis pycom08g02900 pycom15g02830
rosa_chinensis RchiOBHm_Chr6g0300451
rosa_laevigata RLG00000011336
rosa_multiflora Rmu_sc0001670.1_g000004 Rmu_sc0014364.1_g000001
rosa_roxburghii Rroxscaffold_7G00167770
rosa_rugosa Rorug06G0296200
rosa_samantha Rh6AG408800 Rh6BG416000 Rh6CG423500 Rh6DG409200
rosa_wichuraiana Rw6G035770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 229, 1907
Acc36I ACCTGC 2 cut(s) 229, 1907
Acc65I GGTACC 1 cut(s) 1221
AccB1I GGYRCC 4 cut(s) 1221, 1851, 2150, 2246
AccB7I CCANNNNNTGG 2 cut(s) 491, 746
AccII CGCG 2 cut(s) 1101, 2297
AciI CCGC 9 cut(s) 16, 199, 628, 1049, 1101, 1264, 1991, 2053, 2295
AclWI GGATC 2 cut(s) 473, 899
AcoI YGGCCR 2 cut(s) 652, 1526
AcsI RAATTY 4 cut(s) 300, 1063, 1280, 2048
AcuI CTGAAG 5 cut(s) 726, 864, 1389, 1706, 1725
AcvI CACGTG 2 cut(s) 680, 1019
AcyI GRCGYC 2 cut(s) 19, 2247
AdeI CACNNNGTG 1 cut(s) 2174
AfiI CCNNNNNNNGG 8 cut(s) 491, 559, 617, 746, 762, 763, 1709, 1799
AflIII ACRYGT 2 cut(s) 811, 1018
AgsI TTSAA 8 cut(s) 589, 608, 692, 1035, 1185, 1828, 1844, 2327
AhdI GACNNNNNGTC 1 cut(s) 2360
AjiI CACGTC 1 cut(s) 1922
AjnI CCWGG 7 cut(s) 208, 448, 654, 1224, 1729, 2130, 2239
AleI CACNNNNGTG 3 cut(s) 239, 1650, 2022
Alw21I GWGCWC 2 cut(s) 1083, 2170
Alw26I GTCTC 3 cut(s) 199, 1395, 1827
Alw44I GTGCAC 1 cut(s) 2166
AlwI GGATC 2 cut(s) 473, 899
AlwNI CAGNNNCTG 1 cut(s) 2283
Ama87I CYCGRG 1 cut(s) 1903
AoxI GGCC 7 cut(s) 338, 652, 1227, 1261, 1526, 1783, 2373
ApaLI GTGCAC 1 cut(s) 2166
ApeKI GCWGC 6 cut(s) 1929, 2010, 2055, 2191, 2194, 2302
ApoI RAATTY 4 cut(s) 300, 1063, 1280, 2048
Asp718I GGTACC 1 cut(s) 1221
AspLEI GCGC 2 cut(s) 1523, 2249
AspS9I GGNCC 3 cut(s) 1261, 1715, 2422
AsuC2I CCSGG 2 cut(s) 1757, 2251
AsuHPI GGTGA 8 cut(s) 19, 266, 488, 506, 739, 1009, 1559, 1754
AvaI CYCGRG 1 cut(s) 1903
AvaII GGWCC 2 cut(s) 1715, 2422
BaeGI GKGCMC 2 cut(s) 1420, 2170
BalI TGGCCA 1 cut(s) 654
BanI GGYRCC 4 cut(s) 1221, 1851, 2150, 2246
BanII GRGCYC 1 cut(s) 1083
BauI CACGAG 1 cut(s) 1002
BbrPI CACGTG 2 cut(s) 680, 1019
Bbv12I GWGCWC 2 cut(s) 1083, 2170
BbvI GCAGC 6 cut(s) 1916, 2022, 2042, 2203, 2206, 2314
BccI CCATC 8 cut(s) 653, 840, 1082, 1212, 1490, 1676, 2030, 2235
BceAI ACGGC 2 cut(s) 32, 1541
BcgI CGANNNNNNTGC 4 cut(s) 536, 570, 1462, 1496
BciT130I CCWGG 7 cut(s) 210, 450, 656, 1226, 1731, 2132, 2241
BciVI GTATCC 1 cut(s) 1159
BclI TGATCA 1 cut(s) 784
BcnI CCSGG 2 cut(s) 1757, 2251
BcoDI GTCTC 3 cut(s) 199, 1395, 1827
BfaI CTAG 4 cut(s) 566, 1205, 1569, 1865
BfmI CTRYAG 2 cut(s) 576, 1774
BfoI RGCGCY 1 cut(s) 2250
BfuAI ACCTGC 2 cut(s) 229, 1907
BfuI GTATCC 1 cut(s) 1159
BglII AGATCT 1 cut(s) 1172
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 9 cut(s) 210, 450, 656, 1226, 1731, 1757, 2132, 2241, 2251
Bme18I GGWCC 2 cut(s) 1715, 2422
BmeRI GACNNNNNGTC 1 cut(s) 2360
BmeT110I CYCGRG 1 cut(s) 1903
BmgBI CACGTC 1 cut(s) 1922
BmgT120I GGNCC 3 cut(s) 1261, 1715, 2422
BmiI GGNNCC 8 cut(s) 429, 1223, 1262, 1717, 1853, 2152, 2248, 2275
BmrFI CCNGG 9 cut(s) 210, 450, 656, 1226, 1731, 1757, 2132, 2241, 2251
BmrI ACTGGG 4 cut(s) 756, 1129, 1405, 2166
BmsI GCATC 2 cut(s) 355, 2274
BmuI ACTGGG 4 cut(s) 756, 1129, 1405, 2166
Bpu10I CCTNAGC 1 cut(s) 598
BpuEI CTTGAG 2 cut(s) 44, 2219
BpuMI CCSGG 2 cut(s) 1757, 2251
Bsa29I ATCGAT 1 cut(s) 2321
BsaAI YACGTR 3 cut(s) 680, 1019, 1595
BsaHI GRCGYC 2 cut(s) 19, 2247
BsaI GGTCTC 2 cut(s) 199, 1395
BsaJI CCNNGG 4 cut(s) 516, 1270, 1624, 1729
BsaXI ACNNNNNCTCC 4 cut(s) 196, 226, 1824, 1854
Bsc4I CCNNNNNNNGG 8 cut(s) 491, 559, 617, 746, 762, 763, 1709, 1799
Bse1I ACTGG 8 cut(s) 587, 751, 1075, 1124, 1411, 1464, 2106, 2172
Bse3DI GCAATG 2 cut(s) 1321, 2012
BseBI CCWGG 7 cut(s) 210, 450, 656, 1226, 1731, 2132, 2241
BseCI ATCGAT 1 cut(s) 2321
BseDI CCNNGG 4 cut(s) 516, 1270, 1624, 1729
BseGI GGATG 9 cut(s) 526, 664, 937, 982, 1223, 1352, 1640, 1687, 2209
BseLI CCNNNNNNNGG 8 cut(s) 491, 559, 617, 746, 762, 763, 1709, 1799
BseMI GCAATG 2 cut(s) 1321, 2012
BseMII CTCAG 2 cut(s) 52, 1050
BseNI ACTGG 8 cut(s) 587, 751, 1075, 1124, 1411, 1464, 2106, 2172
BseRI GAGGAG 2 cut(s) 217, 816
BseSI GKGCMC 2 cut(s) 1420, 2170
BseXI GCAGC 6 cut(s) 1916, 2022, 2042, 2203, 2206, 2314
BseYI CCCAGC 1 cut(s) 530
BsgI GTGCAG 1 cut(s) 496
Bsh1236I CGCG 2 cut(s) 1101, 2297
Bsh1285I CGRYCG 1 cut(s) 1386
BshFI GGCC 7 cut(s) 340, 654, 1229, 1263, 1528, 1785, 2375
BshNI GGYRCC 4 cut(s) 1221, 1851, 2150, 2246
BshVI ATCGAT 1 cut(s) 2321
BsiEI CGRYCG 1 cut(s) 1386
BsiHKAI GWGCWC 2 cut(s) 1083, 2170
BsiHKCI CYCGRG 1 cut(s) 1903
BsiSI CCGG 3 cut(s) 1757, 2154, 2251
BslFI GGGAC 3 cut(s) 15, 1438, 2360
BslI CCNNNNNNNGG 8 cut(s) 491, 559, 617, 746, 762, 763, 1709, 1799
BsmAI GTCTC 3 cut(s) 199, 1395, 1827
BsmFI GGGAC 3 cut(s) 15, 1438, 2360
BsmI GAATGC 3 cut(s) 174, 1030, 1052
BsnI GGCC 7 cut(s) 340, 654, 1229, 1263, 1528, 1785, 2375
Bso31I GGTCTC 2 cut(s) 199, 1395
BsoBI CYCGRG 1 cut(s) 1903
Bsp1286I GDGCHC 3 cut(s) 1083, 1420, 2170
Bsp1407I TGTACA 4 cut(s) 222, 316, 667, 1349
Bsp143I GATC 5 cut(s) 478, 784, 904, 1172, 1383
BspACI CCGC 9 cut(s) 16, 199, 628, 1049, 1101, 1264, 1991, 2053, 2295
BspANI GGCC 7 cut(s) 340, 654, 1229, 1263, 1528, 1785, 2375
BspCNI CTCAG 2 cut(s) 51, 1051
BspDI ATCGAT 1 cut(s) 2321
BspFNI CGCG 2 cut(s) 1101, 2297
BspHI TCATGA 1 cut(s) 178
BspLI GGNNCC 8 cut(s) 429, 1223, 1262, 1717, 1853, 2152, 2248, 2275
BspMAI CTGCAG 2 cut(s) 580, 1778
BspMI ACCTGC 2 cut(s) 229, 1907
BspPI GGATC 2 cut(s) 473, 899
BspT107I GGYRCC 4 cut(s) 1221, 1851, 2150, 2246
BspTNI GGTCTC 2 cut(s) 199, 1395
BsrDI GCAATG 2 cut(s) 1321, 2012
BsrGI TGTACA 4 cut(s) 222, 316, 667, 1349
BsrI ACTGG 8 cut(s) 587, 751, 1075, 1124, 1411, 1464, 2106, 2172
BssECI CCNNGG 4 cut(s) 516, 1270, 1624, 1729
BssMI GATC 5 cut(s) 478, 784, 904, 1172, 1383
BssNI GRCGYC 2 cut(s) 19, 2247
BssSI CACGAG 1 cut(s) 1002
BssT1I CCWWGG 3 cut(s) 516, 1270, 1624
Bst2BI CACGAG 1 cut(s) 1002
Bst2UI CCWGG 7 cut(s) 210, 450, 656, 1226, 1731, 2132, 2241
Bst4CI ACNGT 9 cut(s) 121, 229, 1379, 1397, 1838, 1859, 1913, 2165, 2285
Bst6I CTCTTC 1 cut(s) 687
BstACI GRCGYC 2 cut(s) 19, 2247
BstAPI GCANNNNNTGC 2 cut(s) 1980, 2402
BstAUI TGTACA 4 cut(s) 222, 316, 667, 1349
BstBAI YACGTR 3 cut(s) 680, 1019, 1595
BstC8I GCNNGC 5 cut(s) 775, 1420, 2071, 2307, 2311
BstDEI CTNAG 3 cut(s) 38, 598, 1059
BstENI CCTNNNNNAGG 1 cut(s) 557
BstF5I GGATG 9 cut(s) 526, 664, 937, 982, 1223, 1352, 1640, 1687, 2209
BstFNI CGCG 2 cut(s) 1101, 2297
BstH2I RGCGCY 1 cut(s) 2250
BstHHI GCGC 2 cut(s) 1523, 2249
BstKTI GATC 5 cut(s) 481, 787, 907, 1175, 1386
BstMAI GTCTC 3 cut(s) 199, 1395, 1827
BstMBI GATC 5 cut(s) 478, 784, 904, 1172, 1383
BstMCI CGRYCG 1 cut(s) 1386
BstMWI GCNNNNNNNGC 7 cut(s) 98, 427, 1782, 1980, 2079, 2088, 2402
BstNI CCWGG 7 cut(s) 210, 450, 656, 1226, 1731, 2132, 2241
BstNSI RCATGY 3 cut(s) 815, 893, 2400
BstSCI CCNGG 9 cut(s) 208, 448, 654, 1224, 1729, 1755, 2130, 2239, 2249
BstSFI CTRYAG 2 cut(s) 576, 1774
BstSLI GKGCMC 2 cut(s) 1420, 2170
BstSNI TACGTA 1 cut(s) 1595
BstUI CGCG 2 cut(s) 1101, 2297
BstV1I GCAGC 6 cut(s) 1916, 2022, 2042, 2203, 2206, 2314
BstX2I RGATCY 1 cut(s) 1172
BstXI CCANNNNNNTGG 1 cut(s) 1631
BstYI RGATCY 1 cut(s) 1172
Bsu15I ATCGAT 1 cut(s) 2321
BsuI GTATCC 1 cut(s) 1159
BsuRI GGCC 7 cut(s) 340, 654, 1229, 1263, 1528, 1785, 2375
BsuTUI ATCGAT 1 cut(s) 2321
BtgZI GCGATG 1 cut(s) 423
BtrI CACGTC 1 cut(s) 1922
BtsCI GGATG 9 cut(s) 526, 664, 937, 982, 1223, 1352, 1640, 1687, 2209
BtsI GCAGTG 2 cut(s) 774, 1986
BveI ACCTGC 2 cut(s) 229, 1907
Cac8I GCNNGC 5 cut(s) 775, 1420, 2071, 2307, 2311
CaiI CAGNNNCTG 1 cut(s) 2283
CciI TCATGA 1 cut(s) 178
CfoI GCGC 2 cut(s) 1523, 2249
Cfr13I GGNCC 3 cut(s) 1261, 1715, 2422
ClaI ATCGAT 1 cut(s) 2321
CseI GACGC 2 cut(s) 8, 1107
CsiI ACCWGGT 1 cut(s) 208
CspCI CAANNNNNGTGG 2 cut(s) 1158, 1193
DdeI CTNAG 3 cut(s) 38, 598, 1059
DinI GGCGCC 1 cut(s) 2248
DpnI GATC 5 cut(s) 480, 786, 906, 1174, 1385
DpnII GATC 5 cut(s) 478, 784, 904, 1172, 1383
DraI TTTAAA 1 cut(s) 357
DraIII CACNNNGTG 1 cut(s) 2174
DriI GACNNNNNGTC 1 cut(s) 2360
EaeI YGGCCR 2 cut(s) 652, 1526
Eam1104I CTCTTC 1 cut(s) 687
Eam1105I GACNNNNNGTC 1 cut(s) 2360
EarI CTCTTC 1 cut(s) 687
Ecl136II GAGCTC 1 cut(s) 1081
Eco105I TACGTA 1 cut(s) 1595
Eco130I CCWWGG 3 cut(s) 516, 1270, 1624
Eco147I AGGCCT 2 cut(s) 340, 1785
Eco24I GRGCYC 1 cut(s) 1083
Eco31I GGTCTC 2 cut(s) 199, 1395
Eco32I GATATC 2 cut(s) 1040, 2319
Eco47I GGWCC 2 cut(s) 1715, 2422
Eco53kI GAGCTC 1 cut(s) 1081
Eco57I CTGAAG 5 cut(s) 726, 864, 1389, 1706, 1725
Eco72I CACGTG 2 cut(s) 680, 1019
Eco88I CYCGRG 1 cut(s) 1903
EcoICRI GAGCTC 1 cut(s) 1081
EcoNI CCTNNNNNAGG 1 cut(s) 557
EcoRI GAATTC 1 cut(s) 2048
EcoRII CCWGG 7 cut(s) 208, 448, 654, 1224, 1729, 2130, 2239
EcoRV GATATC 2 cut(s) 1040, 2319
EcoT14I CCWWGG 3 cut(s) 516, 1270, 1624
EcoT22I ATGCAT 3 cut(s) 891, 916, 1032
EcoT38I GRGCYC 1 cut(s) 1083
EgeI GGCGCC 1 cut(s) 2248
EheI GGCGCC 1 cut(s) 2248
ErhI CCWWGG 3 cut(s) 516, 1270, 1624
FalI AAGNNNNNCTT 2 cut(s) 581, 613
FaqI GGGAC 3 cut(s) 15, 1438, 2360
FbaI TGATCA 1 cut(s) 784
FokI GGATG 9 cut(s) 533, 671, 944, 989, 1230, 1359, 1647, 1694, 2216
FriOI GRGCYC 1 cut(s) 1083
FspBI CTAG 4 cut(s) 566, 1205, 1569, 1865
GlaI GCGC 2 cut(s) 1522, 2248
GsaI CCCAGC 1 cut(s) 534
HaeII RGCGCY 1 cut(s) 2250
HaeIII GGCC 7 cut(s) 340, 654, 1229, 1263, 1528, 1785, 2375
HapII CCGG 3 cut(s) 1757, 2154, 2251
HgaI GACGC 2 cut(s) 8, 1107
HhaI GCGC 2 cut(s) 1523, 2249
Hin1I GRCGYC 2 cut(s) 19, 2247
Hin6I GCGC 2 cut(s) 1521, 2247
HinP1I GCGC 2 cut(s) 1521, 2247
HincII GTYRAC 1 cut(s) 2143
HindII GTYRAC 1 cut(s) 2143
HindIII AAGCTT 3 cut(s) 419, 983, 2255
HinfI GANTC 7 cut(s) 42, 445, 1000, 1249, 2040, 2323, 2355
HpaII CCGG 3 cut(s) 1757, 2154, 2251
HphI GGTGA 8 cut(s) 19, 266, 488, 506, 739, 1009, 1559, 1754
Hpy188III TCNNGA 8 cut(s) 179, 383, 589, 1035, 1253, 1306, 1344, 1703
Hpy99I CGWCG 2 cut(s) 24, 53
HpyCH4III ACNGT 9 cut(s) 121, 229, 1379, 1397, 1838, 1859, 1913, 2165, 2285
HpyCH4IV ACGT 6 cut(s) 679, 781, 1018, 1594, 1921, 2139
HpyF10VI GCNNNNNNNGC 7 cut(s) 98, 427, 1782, 1980, 2079, 2088, 2402
HpyF3I CTNAG 3 cut(s) 38, 598, 1059
HpySE526I ACGT 6 cut(s) 679, 781, 1018, 1594, 1921, 2139
Hsp92I GRCGYC 2 cut(s) 19, 2247
HspAI GCGC 2 cut(s) 1521, 2247
KasI GGCGCC 1 cut(s) 2246
KpnI GGTACC 1 cut(s) 1225
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 5 cut(s) 478, 784, 904, 1172, 1383
LmnI GCTCC 4 cut(s) 427, 829, 1988, 2103
Lsp1109I GCAGC 6 cut(s) 1916, 2022, 2042, 2203, 2206, 2314
LweI GCATC 2 cut(s) 355, 2274
MabI ACCWGGT 1 cut(s) 208
MaeI CTAG 4 cut(s) 566, 1205, 1569, 1865
MaeII ACGT 6 cut(s) 679, 781, 1018, 1594, 1921, 2139
MalI GATC 5 cut(s) 480, 786, 906, 1174, 1385
MboI GATC 5 cut(s) 478, 784, 904, 1172, 1383
MboII GAAGA 5 cut(s) 704, 713, 1097, 1220, 2260
MfeI CAATTG 2 cut(s) 801, 1337
MflI RGATCY 1 cut(s) 1172
MhlI GDGCHC 3 cut(s) 1083, 1420, 2170
MlsI TGGCCA 1 cut(s) 654
MluNI TGGCCA 1 cut(s) 654
Mly113I GGCGCC 1 cut(s) 2247
MlyI GAGTC 3 cut(s) 994, 2049, 2349
MmeI TCCRAC 1 cut(s) 66
Mox20I TGGCCA 1 cut(s) 654
Mph1103I ATGCAT 3 cut(s) 891, 916, 1032
MscI TGGCCA 1 cut(s) 654
MseI TTAA 5 cut(s) 333, 356, 1656, 1737, 2112
MslI CAYNNNNRTG 4 cut(s) 239, 816, 1650, 2022
Msp20I TGGCCA 1 cut(s) 654
MspA1I CMGCKG 3 cut(s) 199, 842, 2055
MspI CCGG 3 cut(s) 1757, 2154, 2251
MspR9I CCNGG 9 cut(s) 210, 450, 656, 1226, 1731, 1757, 2132, 2241, 2251
MunI CAATTG 2 cut(s) 801, 1337
Mva1269I GAATGC 3 cut(s) 174, 1030, 1052
MvaI CCWGG 7 cut(s) 210, 450, 656, 1226, 1731, 2132, 2241
MvnI CGCG 2 cut(s) 1101, 2297
MwoI GCNNNNNNNGC 7 cut(s) 98, 427, 1782, 1980, 2079, 2088, 2402
NarI GGCGCC 1 cut(s) 2247
NciI CCSGG 2 cut(s) 1757, 2251
NdeII GATC 5 cut(s) 478, 784, 904, 1172, 1383
NlaIV GGNNCC 8 cut(s) 429, 1223, 1262, 1717, 1853, 2152, 2248, 2275
NmuCI GTSAC 7 cut(s) 374, 494, 727, 997, 1113, 1285, 2285
NsiI ATGCAT 3 cut(s) 891, 916, 1032
NspI RCATGY 3 cut(s) 815, 893, 2400
OliI CACNNNNGTG 3 cut(s) 239, 1650, 2022
PagI TCATGA 1 cut(s) 178
PaqCI CACCTGC 2 cut(s) 229, 1907
PceI AGGCCT 2 cut(s) 340, 1785
PciI ACATGT 1 cut(s) 811
PctI GAATGC 3 cut(s) 174, 1030, 1052
PfeI GAWTC 4 cut(s) 42, 445, 1249, 2323
PflFI GACNNNGTC 3 cut(s) 210, 1156, 1806
PflMI CCANNNNNTGG 2 cut(s) 491, 746
PfoI TCCNGGA 1 cut(s) 2130
Ple19I CGATCG 1 cut(s) 1386
PleI GAGTC 3 cut(s) 994, 2048, 2349
PluTI GGCGCC 1 cut(s) 2250
PmaCI CACGTG 2 cut(s) 680, 1019
PmlI CACGTG 2 cut(s) 680, 1019
PpsI GAGTC 3 cut(s) 994, 2048, 2349
Ppu21I YACGTR 3 cut(s) 680, 1019, 1595
PscI ACATGT 1 cut(s) 811
Psp124BI GAGCTC 1 cut(s) 1083
Psp6I CCWGG 7 cut(s) 208, 448, 654, 1224, 1729, 2130, 2239
PspCI CACGTG 2 cut(s) 680, 1019
PspFI CCCAGC 1 cut(s) 530
PspGI CCWGG 7 cut(s) 208, 448, 654, 1224, 1729, 2130, 2239
PspN4I GGNNCC 8 cut(s) 429, 1223, 1262, 1717, 1853, 2152, 2248, 2275
PspPI GGNCC 3 cut(s) 1261, 1715, 2422
PstI CTGCAG 2 cut(s) 580, 1778
PstNI CAGNNNCTG 1 cut(s) 2283
PsuI RGATCY 1 cut(s) 1172
PsyI GACNNNGTC 3 cut(s) 210, 1156, 1806
PvuI CGATCG 1 cut(s) 1386
PvuII CAGCTG 1 cut(s) 842
RseI CAYNNNNRTG 4 cut(s) 239, 816, 1650, 2022
SacI GAGCTC 1 cut(s) 1083
SaqAI TTAA 5 cut(s) 333, 356, 1656, 1737, 2112
Sau3AI GATC 5 cut(s) 478, 784, 904, 1172, 1383
Sau96I GGNCC 3 cut(s) 1261, 1715, 2422
ScaI AGTACT 1 cut(s) 311
SchI GAGTC 3 cut(s) 994, 2049, 2349
ScrFI CCNGG 9 cut(s) 210, 450, 656, 1226, 1731, 1757, 2132, 2241, 2251
SduI GDGCHC 3 cut(s) 1083, 1420, 2170
SexAI ACCWGGT 1 cut(s) 208
SfaNI GCATC 2 cut(s) 355, 2274
SfcI CTRYAG 2 cut(s) 576, 1774
SfoI GGCGCC 1 cut(s) 2248
SinI GGWCC 2 cut(s) 1715, 2422
SmiI ATTTAAAT 1 cut(s) 357
SmiMI CAYNNNNRTG 4 cut(s) 239, 816, 1650, 2022
SmlI CTYRAG 2 cut(s) 59, 2198
SmoI CTYRAG 2 cut(s) 59, 2198
SnaBI TACGTA 1 cut(s) 1595
SseBI AGGCCT 2 cut(s) 340, 1785
SsiI CCGC 9 cut(s) 16, 199, 628, 1049, 1101, 1264, 1991, 2053, 2295
SspDI GGCGCC 1 cut(s) 2246
SspMI CTAG 4 cut(s) 566, 1205, 1569, 1865
SstI GAGCTC 1 cut(s) 1083
StuI AGGCCT 2 cut(s) 340, 1785
StyD4I CCNGG 9 cut(s) 208, 448, 654, 1224, 1729, 1755, 2130, 2239, 2249
StyI CCWWGG 3 cut(s) 516, 1270, 1624
SwaI ATTTAAAT 1 cut(s) 357
TaaI ACNGT 9 cut(s) 121, 229, 1379, 1397, 1838, 1859, 1913, 2165, 2285
TaiI ACGT 6 cut(s) 682, 784, 1021, 1597, 1924, 2142
TaqI TCGA 6 cut(s) 397, 443, 1083, 1616, 2321, 2412
TatI WGTACW 6 cut(s) 222, 309, 316, 667, 1349, 1505
TauI GCSGC 4 cut(s) 19, 1052, 1104, 1266
TfiI GAWTC 4 cut(s) 42, 445, 1249, 2323
Tru1I TTAA 5 cut(s) 333, 356, 1656, 1737, 2112
Tru9I TTAA 5 cut(s) 333, 356, 1656, 1737, 2112
TseFI GTSAC 7 cut(s) 374, 494, 727, 997, 1113, 1285, 2285
TseI GCWGC 6 cut(s) 1929, 2010, 2055, 2191, 2194, 2302
Tsp45I GTSAC 7 cut(s) 374, 494, 727, 997, 1113, 1285, 2285
TspDTI ATGAA 9 cut(s) 167, 948, 993, 1154, 1158, 1572, 1653, 1782, 1862
TspGWI ACGGA 2 cut(s) 2034, 2348
Tth111I GACNNNGTC 3 cut(s) 210, 1156, 1806
Van91I CCANNNNNTGG 2 cut(s) 491, 746
VneI GTGCAC 1 cut(s) 2166
VpaK11BI GGWCC 2 cut(s) 1715, 2422
XagI CCTNNNNNAGG 1 cut(s) 557
XapI RAATTY 4 cut(s) 300, 1063, 1280, 2048
XceI RCATGY 3 cut(s) 815, 893, 2400
XcmI CCANNNNNNNNNTGG 1 cut(s) 1237
XspI CTAG 4 cut(s) 566, 1205, 1569, 1865
ZrmI AGTACT 1 cut(s) 311
Zsp2I ATGCAT 3 cut(s) 891, 916, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.