Rh6BG416000

Leishmanolysin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010930
Physical Location & Seq
Reverse (-)
64109640 .. 64116470
6831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG416000.1

Sequence Viewer

Length: 2166 bp
ATGGAGGCAATGTTACGGTGTACTCCATGTCTCGCTCTCAGATTCGGCTCTAAGCTGCGACTCGCCGTCGTTGTCCTCGAGATTGTATTGCTATTTACATGGTTGGAAGCTACTAATGCACAATCTCAAGAGATCATACTGGGAGGGCAAGGCCCGGAGAGGTCGTCAGAGAACATTGTTTCTCACTCCTGCATCCATGACCAGATACTCAAACAACGAAGGCGACCTGGTCGCAAGGTGTACACTGTTACTCCACAGGTGTATGAGGCTTCTGGTATCTCAAAACCCCTTCACCAAAAGGGTAGGGCATTGCTTGGAATTAAAAAGCAGTCTGTACAGCAGAAGGATGCAAAACTGCCCATTAGAATTTTTCTAAATTATGATGCTGTTGGTCACTCGCCTGATAGGGACTGTCGGAATGTCGGGGATATTGTGAAGCTTGGTGAGCCTCCTATTGGTTATCTTTCTGGCTCTCCTTCCTGTAATCCTCATGGGGATCCTCCAGTTTCTGGTGACTGCTGGTATAACTGCACTTTGGACGATATAGCTGGGAAGGACAAAAGGCAACGCCTTAGAAAGGCTCTAGGACAGACAGCAGACTGGTTCAGGAGAGCCTTAGCTGTTGAACCTGTGAGAGGGAACTTGCGGTTGAGTGGATATTCAGCATGCGGTCAAGATGGGGGCGTACAGCTGCCACGTGAATATGTTGAAGAGGGTGTTGCTGAAGCAGACCTCGTTCTACTGGTTACAACAAGACCTACCACCGGTAATACCCTTGCATGGGCAGTGGCTTGTGAACGTGATCAATGGGGTCGTGCGATTGCAGGACATGTAAATGTTGCTCCTCGTCATTTGACAGCTGAAGCAGAAACTTTGCTTTCTGCTACTCTAATACATGAGGTCATGCATGTTCTTGGTTTTGATCCCCATGCCTTTGCTCATTTTAGGGATGAGAGGAAAAGACGGCGTAGTCAGGTTACAGAACAAGTCATGGATGAAAAGCTTGGGCGGATGGTAACCCGTGTGGTGCTTCCACGGGTTGTCATGCATTCACGATATCATTATGCGGCATTCTCTGAGAATTTTACTGGTTTAGAGCTAGAAGATGGGGGAGGGCGTGGCACATCAGGGTCACACTGGGAAAAAAGACTTCTAATGAATGAAATTATGACGGGTTCAGTGGATACAAGATCCGTGGTTTCAAAAATGACACTGGCCTTATTAGAAGATAGTGGATGGTACCATGCTAATTATAGTATGGCAGACAATCTTGATTGGGGTCGCAACCAAGGAACTGAGTTTGTTACTTCCCCTTGCAACGTCTGGAAAGGGGCATATCATTGCAACACAACACAATTGTCAGGATGTACGTACAACAGAGAAGCAGAGGGGTACTGTCCAATTGTAAGTTACAGTGGGGACCTGCCCCAGTGGGCACGCTATTTTCCACAGGCTAACAAAGGTGGACAGTCTTCGCTGGCTGATTATTGCACCTATTTCGTAGCTTACTCTGATGGATCATGTACAGACACTAACAGTGCACGACCACCTGACAGAATGTTGGGTGAAGTACGAGGGAGCAACTCTAGGTGTATGGCCTCATCATTAGTGCGTTCTGGGTTTGTGAGGGGTTCTATGACCCAAGGAAATGGATGTTATCAGCACAGATGTGTCAACAACTCGTTAGAGGTTGCTGTGGATGGGATGTGGAAAGTATGTCCTGAAGCTGGTGGACAAATTCAGTTCCCAGGATTTAATGGTGAATTGATATGCCCGGCATACCATGAACTCTGTGGTACTGGGATAGTTCCCGCGACAGGGCAATGTCCAAATTCTTGTAATTTGAATGGCGACTGTGTTGAAGGAAGATGCCACTGTTTTCTAGGGTTCCATGGTTCTGATTGTAGCAAACGTAAGCTTCTTGTTCCTATCCATCCCTTTATTATCATCACACAACAATCAACACTCAACAAATGTCTGAGCTGCCAACTTCTTAGAATAGTTTTGAATTTATGTTCTATGCAGGCTCCTGTCCCAGCAACTGTAGTGGACGTGGAAACTGCCTATCTAACGGGATATGTGAATGTAGAAATGGGTACACTGGCATTGATTGCTCCACTGGTAATGCTTTTCTTTCTTGTCTGTTGTTTTTTTTTTCCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000819 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006323 GO:0006325 GO:0006338 GO:0006508 GO:0006807 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007076 GO:0007088 GO:0007098 GO:0007100 GO:0007275 GO:0007276 GO:0007346 GO:0007399 GO:0007417 GO:0007420 GO:0007444 GO:0007548 GO:0008150 GO:0008152 GO:0008233 GO:0008354 GO:0008406 GO:0009888 GO:0009896 GO:0009987 GO:0010564 GO:0010638 GO:0010965 GO:0016043 GO:0016477 GO:0016787 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030071 GO:0030261 GO:0031023 GO:0031331 GO:0032270 GO:0032436 GO:0032501 GO:0032502 GO:0032504 GO:0033043 GO:0033044 GO:0033045 GO:0033047 GO:0035295 GO:0040011 GO:0043170 GO:0044238 GO:0044424 GO:0044464 GO:0044703 GO:0045137 GO:0045732 GO:0045787 GO:0045840 GO:0045842 GO:0045862 GO:0045931 GO:0048285 GO:0048513 GO:0048518 GO:0048522 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0048870 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051247 GO:0051276 GO:0051298 GO:0051299 GO:0051674 GO:0051704 GO:0051726 GO:0051781 GO:0051783 GO:0051785 GO:0051983 GO:0051984 GO:0060322 GO:0060429 GO:0061458 GO:0062033 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0090068 GO:0098813 GO:0140014 GO:0140096 GO:1901564 GO:1901800 GO:1901970 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902099 GO:1902101 GO:1902850 GO:1903047 GO:1903052 GO:1903364 GO:1905818 GO:1905820 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko05140 ko05143 map05140 map05143
Pfam Domains
Protein Families
EGF_2 Peptidase_M8

Protein Analysis

721

Amino Acids

79.54

Weight (kDa)

7.85

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M8 PF01457 175 - 599 1e-81 Leishmanolysin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42620 AT5G42620
fragaria_vesca FvH4_2g40290 FvH4_2g40290 FvH4_2g40290
malus_domestica MD08G1036300.v1.1
prunus_persica Prupe.1G383600_v2.0.a1
pyrus_communis pycom08g02900 pycom15g02830
rosa_chinensis RchiOBHm_Chr6g0300451
rosa_laevigata RLG00000011336
rosa_multiflora Rmu_sc0001670.1_g000004 Rmu_sc0014364.1_g000001
rosa_roxburghii Rroxscaffold_7G00167770
rosa_rugosa Rorug06G0296200
rosa_samantha Rh6AG408800 Rh6BG416000 Rh6CG423500 Rh6DG409200
rosa_wichuraiana Rw6G035770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 969
Acc36I ACCTGC 1 cut(s) 1431
Acc65I GGTACC 1 cut(s) 1239
AccB1I GGYRCC 1 cut(s) 1239
AccB7I CCANNNNNTGG 1 cut(s) 509
AccII CGCG 1 cut(s) 1814
AciI CCGC 5 cut(s) 646, 669, 1009, 1067, 1812
AclWI GGATC 5 cut(s) 491, 504, 917, 1185, 1525
AcsI RAATTY 5 cut(s) 366, 1081, 1737, 1831, 2008
AcuI CTGAAG 3 cut(s) 744, 882, 1743
AcvI CACGTG 1 cut(s) 698
AflIII ACRYGT 1 cut(s) 829
AgeI ACCGGT 1 cut(s) 764
AgsI TTSAA 6 cut(s) 626, 710, 1203, 1846, 1862, 2008
AhdI GACNNNNNGTC 1 cut(s) 65
AjiI CACGTC 1 cut(s) 2053
AjnI CCWGG 2 cut(s) 226, 1747
AleI CACNNNNGTG 1 cut(s) 1668
Alw21I GWGCWC 1 cut(s) 1543
Alw26I GTCTC 1 cut(s) 35
Alw44I GTGCAC 1 cut(s) 1539
AlwI GGATC 5 cut(s) 491, 504, 917, 1185, 1525
AlwNI CAGNNNCTG 2 cut(s) 509, 2042
Ama87I CYCGRG 1 cut(s) 77
AoxI GGCC 3 cut(s) 151, 1215, 1596
ApaLI GTGCAC 1 cut(s) 1539
ApeKI GCWGC 3 cut(s) 55, 691, 1983
ApoI RAATTY 5 cut(s) 366, 1081, 1737, 1831, 2008
AsiGI ACCGGT 1 cut(s) 764
Asp718I GGTACC 1 cut(s) 1239
AspS9I GGNCC 2 cut(s) 152, 1420
AsuC2I CCSGG 2 cut(s) 155, 1775
AsuHPI GGTGA 5 cut(s) 284, 455, 524, 1577, 1772
AvaI CYCGRG 1 cut(s) 77
AvaII GGWCC 1 cut(s) 1420
BaeGI GKGCMC 2 cut(s) 1438, 1543
BamHI GGATCC 1 cut(s) 496
BanI GGYRCC 1 cut(s) 1239
BbrPI CACGTG 1 cut(s) 698
BbsI GAAGAC 1 cut(s) 1464
Bbv12I GWGCWC 1 cut(s) 1543
BbvI GCAGC 3 cut(s) 42, 678, 1970
BccI CCATC 7 cut(s) 671, 1006, 1100, 1230, 1508, 1694, 1941
BceAI ACGGC 2 cut(s) 50, 980
BcgI CGANNNNNNTGC 2 cut(s) 1480, 1514
BciT130I CCWGG 2 cut(s) 228, 1749
BciVI GTATCC 1 cut(s) 1177
BclI TGATCA 1 cut(s) 802
BcnI CCSGG 2 cut(s) 155, 1775
BcoDI GTCTC 1 cut(s) 35
BfaI CTAG 4 cut(s) 584, 1100, 1587, 1883
BfmI CTRYAG 1 cut(s) 2043
BfuAI ACCTGC 1 cut(s) 1431
BfuI GTATCC 1 cut(s) 1177
BisI GCNGC 4 cut(s) 56, 692, 1068, 1984
BlsI GCNGC 4 cut(s) 57, 693, 1069, 1985
Bme1390I CCNGG 4 cut(s) 155, 228, 1749, 1775
Bme18I GGWCC 1 cut(s) 1420
BmeRI GACNNNNNGTC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 77
BmgBI CACGTC 1 cut(s) 2053
BmgT120I GGNCC 2 cut(s) 152, 1420
BmiI GGNNCC 5 cut(s) 498, 1241, 1421, 1889, 2028
BmrFI CCNGG 4 cut(s) 155, 228, 1749, 1775
BmrI ACTGGG 4 cut(s) 149, 1147, 1423, 1809
BmsI GCATC 4 cut(s) 201, 337, 373, 1859
BmuI ACTGGG 4 cut(s) 149, 1147, 1423, 1809
BpiI GAAGAC 1 cut(s) 1464
BpmI CTGGAG 1 cut(s) 486
Bpu10I CCTNAGC 1 cut(s) 616
BpuEI CTTGAG 1 cut(s) 111
BpuMI CCSGG 2 cut(s) 155, 1775
BsaAI YACGTR 2 cut(s) 698, 1371
BsaJI CCNNGG 6 cut(s) 1034, 1194, 1288, 1642, 1747, 1891
BsaWI WCCGGW 1 cut(s) 764
BsaXI ACNNNNNCTCC 5 cut(s) 26, 149, 179, 235, 265
Bse118I RCCGGY 1 cut(s) 764
Bse3DI GCAATG 4 cut(s) 15, 308, 1339, 1829
BseBI CCWGG 2 cut(s) 228, 1749
BseDI CCNNGG 6 cut(s) 1034, 1194, 1288, 1642, 1747, 1891
BseMI GCAATG 4 cut(s) 15, 308, 1339, 1829
BseMII CTCAG 4 cut(s) 52, 1068, 1287, 1970
BseRI GAGGAG 1 cut(s) 834
BseSI GKGCMC 2 cut(s) 1438, 1543
BseXI GCAGC 3 cut(s) 42, 678, 1970
BseYI CCCAGC 2 cut(s) 548, 2035
BsgI GTGCAG 1 cut(s) 514
Bsh1236I CGCG 1 cut(s) 1814
BshFI GGCC 3 cut(s) 153, 1217, 1598
BshNI GGYRCC 1 cut(s) 1239
BshTI ACCGGT 1 cut(s) 764
BsiHKAI GWGCWC 1 cut(s) 1543
BsiHKCI CYCGRG 1 cut(s) 77
BsiSI CCGG 3 cut(s) 155, 765, 1775
BslFI GGGAC 3 cut(s) 422, 1433, 2018
BsmAI GTCTC 1 cut(s) 35
BsmFI GGGAC 3 cut(s) 422, 1433, 2018
BsmI GAATGC 2 cut(s) 1048, 1070
BsnI GGCC 3 cut(s) 153, 1217, 1598
BsoBI CYCGRG 1 cut(s) 77
Bsp1286I GDGCHC 2 cut(s) 1438, 1543
Bsp1407I TGTACA 3 cut(s) 240, 334, 1523
Bsp143I GATC 6 cut(s) 132, 496, 802, 922, 1190, 1517
Bsp19I CCATGG 1 cut(s) 1891
BspACI CCGC 5 cut(s) 646, 669, 1009, 1067, 1812
BspANI GGCC 3 cut(s) 153, 1217, 1598
BspCNI CTCAG 4 cut(s) 51, 1069, 1288, 1971
BspFNI CGCG 1 cut(s) 1814
BspLI GGNNCC 5 cut(s) 498, 1241, 1421, 1889, 2028
BspMI ACCTGC 1 cut(s) 1431
BspPI GGATC 5 cut(s) 491, 504, 917, 1185, 1525
BspT107I GGYRCC 1 cut(s) 1239
BsrDI GCAATG 4 cut(s) 15, 308, 1339, 1829
BsrFI RCCGGY 1 cut(s) 764
BsrGI TGTACA 3 cut(s) 240, 334, 1523
BssAI RCCGGY 1 cut(s) 764
BssECI CCNNGG 6 cut(s) 1034, 1194, 1288, 1642, 1747, 1891
BssMI GATC 6 cut(s) 132, 496, 802, 922, 1190, 1517
BssT1I CCWWGG 3 cut(s) 1288, 1642, 1891
Bst2UI CCWGG 2 cut(s) 228, 1749
Bst6I CTCTTC 1 cut(s) 705
BstAPI GCANNNNNTGC 1 cut(s) 2111
BstAUI TGTACA 3 cut(s) 240, 334, 1523
BstBAI YACGTR 2 cut(s) 698, 1371
BstC8I GCNNGC 4 cut(s) 667, 1438, 1479, 2025
BstDEI CTNAG 8 cut(s) 38, 51, 572, 616, 1077, 1296, 1979, 1994
BstDSI CCRYGG 3 cut(s) 1034, 1194, 1891
BstEII GGTNACC 1 cut(s) 1015
BstENI CCTNNNNNAGG 2 cut(s) 575, 633
BstFNI CGCG 1 cut(s) 1814
BstKTI GATC 6 cut(s) 135, 499, 805, 925, 1193, 1520
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 6 cut(s) 132, 496, 802, 922, 1190, 1517
BstMWI GCNNNNNNNGC 3 cut(s) 116, 445, 2111
BstNI CCWGG 2 cut(s) 228, 1749
BstNSI RCATGY 3 cut(s) 669, 833, 911
BstPI GGTNACC 1 cut(s) 1015
BstSCI CCNGG 4 cut(s) 153, 226, 1747, 1773
BstSFI CTRYAG 1 cut(s) 2043
BstSLI GKGCMC 2 cut(s) 1438, 1543
BstSNI TACGTA 1 cut(s) 1371
BstUI CGCG 1 cut(s) 1814
BstV1I GCAGC 3 cut(s) 42, 678, 1970
BstV2I GAAGAC 1 cut(s) 1464
BstX2I RGATCY 2 cut(s) 496, 1190
BstXI CCANNNNNNTGG 1 cut(s) 1649
BstYI RGATCY 2 cut(s) 496, 1190
BsuI GTATCC 1 cut(s) 1177
BsuRI GGCC 3 cut(s) 153, 1217, 1598
BtgI CCRYGG 3 cut(s) 1034, 1194, 1891
BtrI CACGTC 1 cut(s) 2053
BtsI GCAGTG 1 cut(s) 792
BveI ACCTGC 1 cut(s) 1431
Cac8I GCNNGC 4 cut(s) 667, 1438, 1479, 2025
CaiI CAGNNNCTG 2 cut(s) 509, 2042
Cfr10I RCCGGY 1 cut(s) 764
Cfr13I GGNCC 2 cut(s) 152, 1420
CsiI ACCWGGT 1 cut(s) 226
CspAI ACCGGT 1 cut(s) 764
CspCI CAANNNNNGTGG 4 cut(s) 1176, 1211, 2028, 2063
DdeI CTNAG 8 cut(s) 38, 51, 572, 616, 1077, 1296, 1979, 1994
DpnI GATC 6 cut(s) 134, 498, 804, 924, 1192, 1519
DpnII GATC 6 cut(s) 132, 496, 802, 922, 1190, 1517
DrdI GACNNNNNNGTC 1 cut(s) 969
DriI GACNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 969
Eam1104I CTCTTC 1 cut(s) 705
Eam1105I GACNNNNNGTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 705
EciI GGCGGA 1 cut(s) 1024
Eco105I TACGTA 1 cut(s) 1371
Eco130I CCWWGG 3 cut(s) 1288, 1642, 1891
Eco32I GATATC 1 cut(s) 1058
Eco47I GGWCC 1 cut(s) 1420
Eco57I CTGAAG 3 cut(s) 744, 882, 1743
Eco72I CACGTG 1 cut(s) 698
Eco88I CYCGRG 1 cut(s) 77
Eco91I GGTNACC 1 cut(s) 1015
EcoNI CCTNNNNNAGG 2 cut(s) 575, 633
EcoO109I RGGNCCY 1 cut(s) 1420
EcoO65I GGTNACC 1 cut(s) 1015
EcoRII CCWGG 2 cut(s) 226, 1747
EcoRV GATATC 1 cut(s) 1058
EcoT14I CCWWGG 3 cut(s) 1288, 1642, 1891
EcoT22I ATGCAT 2 cut(s) 909, 1050
ErhI CCWWGG 3 cut(s) 1288, 1642, 1891
FaqI GGGAC 3 cut(s) 422, 1433, 2018
FauI CCCGC 1 cut(s) 1819
FbaI TGATCA 1 cut(s) 802
Fnu4HI GCNGC 4 cut(s) 56, 692, 1068, 1984
Fsp4HI GCNGC 4 cut(s) 56, 692, 1068, 1984
FspBI CTAG 4 cut(s) 584, 1100, 1587, 1883
GluI GCNGC 4 cut(s) 56, 692, 1068, 1984
GsaI CCCAGC 2 cut(s) 552, 2039
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 3 cut(s) 153, 1217, 1598
HapII CCGG 3 cut(s) 155, 765, 1775
HincII GTYRAC 1 cut(s) 1675
HindII GTYRAC 1 cut(s) 1675
HindIII AAGCTT 3 cut(s) 437, 1001, 1916
HinfI GANTC 2 cut(s) 42, 60
HpaII CCGG 3 cut(s) 155, 765, 1775
HphI GGTGA 5 cut(s) 284, 455, 524, 1577, 1772
Hpy188I TCNGA 7 cut(s) 41, 169, 417, 1078, 1513, 1900, 1980
Hpy188III TCNNGA 9 cut(s) 79, 128, 607, 674, 1053, 1271, 1324, 1362, 1721
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 6 cut(s) 213, 299, 337, 486, 547, 1856
HpyCH4IV ACGT 6 cut(s) 697, 799, 1320, 1370, 1912, 2052
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 445, 2111
HpyF3I CTNAG 8 cut(s) 38, 51, 572, 616, 1077, 1296, 1979, 1994
HpySE526I ACGT 6 cut(s) 697, 799, 1320, 1370, 1912, 2052
KpnI GGTACC 1 cut(s) 1243
Ksp22I TGATCA 1 cut(s) 802
Kzo9I GATC 6 cut(s) 132, 496, 802, 922, 1190, 1517
LmnI GCTCC 4 cut(s) 847, 1578, 2032, 2119
Lsp1109I GCAGC 3 cut(s) 42, 678, 1970
LweI GCATC 4 cut(s) 201, 337, 373, 1859
MabI ACCWGGT 1 cut(s) 226
MaeI CTAG 4 cut(s) 584, 1100, 1587, 1883
MaeII ACGT 6 cut(s) 697, 799, 1320, 1370, 1912, 2052
MalI GATC 6 cut(s) 134, 498, 804, 924, 1192, 1519
MboI GATC 6 cut(s) 132, 496, 802, 922, 1190, 1517
MboII GAAGA 5 cut(s) 722, 1115, 1238, 1464, 1878
MfeI CAATTG 2 cut(s) 1355, 1401
MflI RGATCY 2 cut(s) 496, 1190
MhlI GDGCHC 2 cut(s) 1438, 1543
MlyI GAGTC 1 cut(s) 54
MmeI TCCRAC 2 cut(s) 84, 395
Mph1103I ATGCAT 2 cut(s) 909, 1050
MseI TTAA 2 cut(s) 321, 1755
MslI CAYNNNNRTG 2 cut(s) 834, 1668
MspA1I CMGCKG 2 cut(s) 691, 860
MspI CCGG 3 cut(s) 155, 765, 1775
MspR9I CCNGG 4 cut(s) 155, 228, 1749, 1775
MunI CAATTG 2 cut(s) 1355, 1401
Mva1269I GAATGC 2 cut(s) 1048, 1070
MvaI CCWGG 2 cut(s) 228, 1749
MvnI CGCG 1 cut(s) 1814
MwoI GCNNNNNNNGC 3 cut(s) 116, 445, 2111
NciI CCSGG 2 cut(s) 155, 1775
NcoI CCATGG 1 cut(s) 1891
NdeII GATC 6 cut(s) 132, 496, 802, 922, 1190, 1517
NlaIV GGNNCC 5 cut(s) 498, 1241, 1421, 1889, 2028
NmuCI GTSAC 3 cut(s) 392, 512, 1131
NsiI ATGCAT 2 cut(s) 909, 1050
NspI RCATGY 3 cut(s) 669, 833, 911
OliI CACNNNNGTG 1 cut(s) 1668
PaeI GCATGC 1 cut(s) 669
PaeR7I CTCGAG 1 cut(s) 77
PciI ACATGT 1 cut(s) 829
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 2 cut(s) 1048, 1070
PfeI GAWTC 1 cut(s) 42
PflFI GACNNNGTC 1 cut(s) 228
PflMI CCANNNNNTGG 1 cut(s) 509
PinAI ACCGGT 1 cut(s) 764
PkrI GCNGC 4 cut(s) 57, 693, 1069, 1985
PleI GAGTC 1 cut(s) 54
PmaCI CACGTG 1 cut(s) 698
PmlI CACGTG 1 cut(s) 698
PpsI GAGTC 1 cut(s) 54
Ppu21I YACGTR 2 cut(s) 698, 1371
PpuMI RGGWCCY 1 cut(s) 1420
PscI ACATGT 1 cut(s) 829
Psp5II RGGWCCY 1 cut(s) 1420
Psp6I CCWGG 2 cut(s) 226, 1747
PspCI CACGTG 1 cut(s) 698
PspEI GGTNACC 1 cut(s) 1015
PspFI CCCAGC 2 cut(s) 548, 2035
PspGI CCWGG 2 cut(s) 226, 1747
PspN4I GGNNCC 5 cut(s) 498, 1241, 1421, 1889, 2028
PspPI GGNCC 2 cut(s) 152, 1420
PspPPI RGGWCCY 1 cut(s) 1420
PstNI CAGNNNCTG 2 cut(s) 509, 2042
PsuI RGATCY 2 cut(s) 496, 1190
PsyI GACNNNGTC 1 cut(s) 228
PvuII CAGCTG 2 cut(s) 691, 860
RseI CAYNNNNRTG 2 cut(s) 834, 1668
SaqAI TTAA 2 cut(s) 321, 1755
SatI GCNGC 4 cut(s) 56, 692, 1068, 1984
Sau3AI GATC 6 cut(s) 132, 496, 802, 922, 1190, 1517
Sau96I GGNCC 2 cut(s) 152, 1420
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 4 cut(s) 155, 228, 1749, 1775
SduI GDGCHC 2 cut(s) 1438, 1543
SexAI ACCWGGT 1 cut(s) 226
SfaNI GCATC 4 cut(s) 201, 337, 373, 1859
SfcI CTRYAG 1 cut(s) 2043
Sfr274I CTCGAG 1 cut(s) 77
SinI GGWCC 1 cut(s) 1420
SlaI CTCGAG 1 cut(s) 77
SmiMI CAYNNNNRTG 2 cut(s) 834, 1668
SmlI CTYRAG 2 cut(s) 77, 126
SmoI CTYRAG 2 cut(s) 77, 126
SnaBI TACGTA 1 cut(s) 1371
SphI GCATGC 1 cut(s) 669
SsiI CCGC 5 cut(s) 646, 669, 1009, 1067, 1812
SspMI CTAG 4 cut(s) 584, 1100, 1587, 1883
StyD4I CCNGG 4 cut(s) 153, 226, 1747, 1773
StyI CCWWGG 3 cut(s) 1288, 1642, 1891
TaiI ACGT 6 cut(s) 700, 802, 1323, 1373, 1915, 2055
TaqI TCGA 1 cut(s) 78
TatI WGTACW 4 cut(s) 20, 240, 334, 1523
TauI GCSGC 1 cut(s) 1070
TfiI GAWTC 1 cut(s) 42
Tru1I TTAA 2 cut(s) 321, 1755
Tru9I TTAA 2 cut(s) 321, 1755
TseFI GTSAC 3 cut(s) 392, 512, 1131
TseI GCWGC 3 cut(s) 55, 691, 1983
Tsp45I GTSAC 3 cut(s) 392, 512, 1131
TspDTI ATGAA 4 cut(s) 1011, 1172, 1176, 1800
TspGWI ACGGA 1 cut(s) 1183
Tth111I GACNNNGTC 1 cut(s) 228
Van91I CCANNNNNTGG 1 cut(s) 509
VneI GTGCAC 1 cut(s) 1539
VpaK11BI GGWCC 1 cut(s) 1420
XagI CCTNNNNNAGG 2 cut(s) 575, 633
XapI RAATTY 5 cut(s) 366, 1081, 1737, 1831, 2008
XceI RCATGY 3 cut(s) 669, 833, 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 1790
XhoI CTCGAG 1 cut(s) 77
XspI CTAG 4 cut(s) 584, 1100, 1587, 1883
Zsp2I ATGCAT 2 cut(s) 909, 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.