Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0013112

Physical Location & Seq

Genomic Coordinates Reverse (-)

4857619 .. 4862520

Gene Structure 4902 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1061500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3150 bp

ATGGAGCGCGCGAGGCGGCTGGCGAACCGGGCTTTCGTGAAGCGGTTGGTCTCGGAGGCCAAGCAGTTCCGCCAGAACGAGAGCTCTGTTCTGAGCTCCTCCACCTCCCCTGTTCTCTACGCACCGTCGAGGTACGTCTCCTCACTCTCCCCATGCTCCTTCACGCGCTCGAATCCCAGATCAGACTCGCTCCCCTCGAGAAACGCGTCCCACAATGCCGGGTTCCAAACCCGGTCGATTTCCGTGGACGCATTGAAAAGCAGCGACACTTTTCCCCGCCGCCATAACTCCGCCACCCCGGAGGAGCAGATTAAAATGGCGGAGTTATGCGGGTTCGATGGCCTCGACTCGCTGATCGACGCCACCGTGCCCAAATCGATTCGACTCGAATCGATGAAGTTTCCGAAATTCGATGAGGGGTTGACAGAGAGTCAAATGCTTGAACACATGAAGAATTTGGCCTCAAAAAACCAGATTTTCAAGAGTTTTATTGGGATGGGATATTACAACACACATGTCCCACCTGTAATTTTGAGGAATATTATGGAAAATCCAGCTTGGTACACTCAGTACACACCTTACCAGGCTGAGATTTCCCAGGGGAGGTTGGAGTCTCTGCTCAATTTCCAAACTTTGATCACTGACCTCACCGGCCTTCCCATGTCGAATGCATCACTGCTCGATGAGGGAACCGCAGCCGCGGAGGCAATGGCGATGTGTAACAACATCCAAAAGGGAAAGAAGAAGACTTTTGTCATTGCCAACAACTGCCACCCCCAGACCATTGATATTTGCAAGACTAGAGCTGATGGTTTCGATCTTAAGGTTGTTACCGCAGATCTTAAGGATGTTGATTACAGTTCCGGTGATGTTTGTGGTGTTCTTGTTCAGTATCCGGGGACTGAGGGCGAGGTTTTGGACTACGGGGAGTTTATTAAGAATGCTCATGCCAATGGGGTTAAGGTTGTGATGGCAACTGATCTGTTGGCGTTGACAGTGTTGAAGCCGCCTGGTGAGTTTGGGGCGGATATTGTTGTTGGTTCTGCGCAGAGGTTTGGTGTTCCGATGGGGTATGGAGGTCCTCATGCGGCTTTCCTGGCCACCTCACAAGAGTACAAGAGGATGATGCCGGGGAGAATTATTGGTGTTAGTGTTGATTCCTCGGGGAAGCCTGCGCTGCGTATGGCTATGCAGACAAGGGAGCAGCATATCAGGAGGGACAAAGCTACCAGCAACATTTGCACTGCTCAGGCATTACTTGCAAACATGGCTGCCATGTACGCTGTATATCATGGACCCGAAGGCCTTAAAACCATTTCTCAAAGGGTTCATGGTCTTGCTGGGGCGTTTGCAGTTGGATTGAAGAAACTTGGAACAGTGGAAGTTCAGAGCCTTCCCTTCTTCGACACTGTGAAGGTTAAGGTTGCTGACGCACATGCAATTTCTGATGCTGCTAACAAGCTTGGATTAAATTTGAGAGTAGTAGACTCGAGCACTATCACTGTTTCGTTTGACGAAACAACTACCTTGGAGGATGTTGACAAGCTTTTCAAAGCTTTTGCATTGGGAAAACCTGTCCCTTTCACTGCTGCATCTCTTGCGCCAGAAGTTCAGCCTGCAATTCCTTCTGGGTTAACGAGGGAGAGCACATTCCTTACCCATCCGATCTTCAACTCGTATCATACCGAGCATGAGTTGCTACGATACATGCATAAGTTGCAATCGAAGGATCTGTCTCTATGCCACAGTATGATTCCCTTGGGATCTTGTACCATGAAATTGAACGCAACAACTGAAATGATGCCAGTGACATGGCCTAGTTTCACCGATATTCACCCTTTTGCTCCTACAGAACAGGCTGAGGGTTATCAGGAAATGTTCAGAGATTTGGGTGACCTATTGTGCACCATCACTGGCTTTGACTCTTTCTCTTTGCAACCCAATGCCGGAGCTGCTGGAGAGTATGCTGGGCTGATGGTTATTCGGGCATATCATTTTGCAAGAGGGGATCGCCACCGGAATGTGTGTATCATTCCTGTCTCGGCACATGGTACGAATCCTGCTAGTGCTGCAATGTGTGGAATGAAGATTGTAACTATTGGAACTGATGCCAAGGGAAACATCAACATTGAAGAGCTGAAGAAGGCTGCTGAAGCAAACAAGGATAACCTATCAGCTTTTATGGTGACATATCCTTCAACTCACGGAGTGTATGAAGAAGGTATTGATGAGATATGTAAGATCATTCATGACAACGGGGGTCAAGTATACATGGACGGGGCTAACATGAATGCACAGGTTGGTCTGACAAGCCCAGGTTGGATTGGAGCTGACGTTTGCCATCTCAATCTCCACAAAACATTTTGCATTCCACACGGAGGTGGTGGCCCTGGCATGGGTCCTATTGGTGTGAAGAAGCACTTGGCACCCTTCTTACCATCCCATCCTGTGGTATCTACCGGTGGCCTTCCTGTCCCTGACAAGTCTCAGCCACTCGGAACCATCTCTGCTGCACCTTGGGGCTCTGCACTTATTTTGCCAATCTCTTATACTTACATAGCCATGATGGGGTCCAAGGGACTCACTGATGCATCAAAGATAGCAATTCTGAATGCAAACTACATGGCCAAACGCTTGGAGAATTACTACCCCATTCTTTTCCGTGGTGTCAATGGAACTGTTGCCCACGAATTCATTGTTGACTTGAGAGGATTTAAGAATACTGCTGGGATAGAGCCTGAGGACGTTGCCAAGCGTCTTATGGACTACGGTTTCCATGGACCAACAATGTCATGGCCAGTTCCTGGCACTCTCATGATTGAGCCAACAGAAAGTGAAAGCAAGGCCGAGTTAGACAGGTTTTGTGATGCTCTAATTTCCATCAGAGAAGAGATTGCTCAGATTGAGAAAGGAAAAGCCGATATTCTCAACAATGTCCTTAAAGGAGCTCCTCACCCGCCATCACTGCTCATGGGAGATGCATGGACAAAGCCATATTCCCGGGAATATGCAGCCTTCCCCGCTTCCTGGCTCCGTTCTTCCAAGTTCTGGCCTACCACAGGACGCGTTGACAATGTGTATGGTGACCGTAACCTCGTCTGCACCCTTCAACCGGAAGCAGTGGAGGAACAAGCAGCAGCCACTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001505 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006520 GO:0006544 GO:0006546 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009069 GO:0009071 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0016054 GO:0017076 GO:0017144 GO:0019752 GO:0030554 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042133 GO:0042135 GO:0042221 GO:0042737 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046395 GO:0046686 GO:0048046 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606

KEGG Pathways

Metabolic & Signaling

ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200

Pfam Domains

Protein Families

GDC-P

Protein Analysis

1050

Amino Acids

114.06

Weight (kDa)

6.52

Isoelectric Point (pI)

45.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GDC-P	PF02347	94 - 520	2.1e-183	Glycine cleavage system P-protein
GDC-P	PF02347	540 - 813	5.4e-10	Glycine cleavage system P-protein
GcvP2_C	PF21478	864 - 985	7.6e-59	Glycine dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013112)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26080 AT4G33010 AT4G33010
fragaria_vesca	FvH4_2g31940
malus_domestica	MD08G1061500.v1.1 MD15G1060200.v1.1
prunus_persica	Prupe.1G412100_v2.0.a1
pyrus_communis	pycom08g05100 pycom15g05650 pycom16g25720
rosa_chinensis	RchiOBHm_Chr6g0310901
rosa_laevigata	RLG00000010452
rosa_multiflora	Rmu_ssc0000395.1_g000001
rosa_roxburghii	Rroxscaffold_7G00158200
rosa_rugosa	Rorug06G0383900
rosa_samantha	Rh6BG507800 Rh6CG513800
rosa_wichuraiana	Rw6G043350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1047
AccB1I	GGYRCC	1 cut(s)	2425
AccB7I	CCANNNNNTGG	3 cut(s)	2449, 2804, 3048
AccI	GTMKAC	2 cut(s)	1485, 2268
AccII	CGCG	6 cut(s)	9, 11, 166, 206, 701, 3066
AclWI	GGATC	3 cut(s)	1737, 1771, 2016
AcoI	YGGCCR	3 cut(s)	1098, 2625, 2795
AcsI	RAATTY	4 cut(s)	407, 454, 1471, 2690
AcuI	CTGAAG	2 cut(s)	2159, 2172
AcyI	GRCGYC	1 cut(s)	360
AdeI	CACNNNGTG	1 cut(s)	2209
AfaI	GTAC	7 cut(s)	134, 563, 572, 1115, 1280, 1771, 2053
AflII	CTTAAG	2 cut(s)	821, 842
AflIII	ACRYGT	3 cut(s)	204, 514, 3064
AgeI	ACCGGT	1 cut(s)	2459
AhdI	GACNNNNNGTC	1 cut(s)	429
AjnI	CCWGG	8 cut(s)	582, 597, 1009, 1095, 2314, 2389, 2803, 3026
AjuI	GAANNNNNNNTTGG	2 cut(s)	2405, 2437
AleI	CACNNNNGTG	1 cut(s)	2379
Alw21I	GWGCWC	6 cut(s)	86, 98, 1496, 1649, 1907, 2950
Alw26I	GTCTC	6 cut(s)	55, 142, 618, 1740, 2044, 2490
Alw44I	GTGCAC	1 cut(s)	1903
AlwI	GGATC	3 cut(s)	1737, 1771, 2016
AlwNI	CAGNNNCTG	2 cut(s)	2804, 3143
Ama87I	CYCGRG	4 cut(s)	196, 1162, 1489, 3000
ApaLI	GTGCAC	1 cut(s)	1903
ApoI	RAATTY	4 cut(s)	407, 454, 1471, 2690
ArsI	GACNNNNNNTTYG	2 cut(s)	1718, 1750
AsiGI	ACCGGT	1 cut(s)	2459
AspLEI	GCGC	6 cut(s)	9, 11, 168, 1048, 1177, 1603
AspS9I	GGNCC	6 cut(s)	1079, 1295, 2387, 2399, 2571, 2780
AsuC2I	CCSGG	8 cut(s)	29, 220, 232, 299, 897, 1131, 3001, 3002
AsuHPI	GGTGA	9 cut(s)	640, 878, 1025, 1816, 1826, 1904, 2197, 2945, 3095
AvaI	CYCGRG	4 cut(s)	196, 1162, 1489, 3000
AvaII	GGWCC	5 cut(s)	1079, 1295, 2399, 2571, 2780
AxyI	CCTNAGG	1 cut(s)	2739
BaeGI	GKGCMC	2 cut(s)	372, 1907
BalI	TGGCCA	3 cut(s)	1100, 2627, 2797
BanI	GGYRCC	1 cut(s)	2425
BanII	GRGCYC	4 cut(s)	86, 98, 2525, 2950
BbsI	GAAGAC	1 cut(s)	752
Bbv12I	GWGCWC	6 cut(s)	86, 98, 1496, 1649, 1907, 2950
BbvCI	CCTCAGC	1 cut(s)	1860
BciT130I	CCWGG	8 cut(s)	584, 599, 1011, 1097, 2316, 2391, 2805, 3028
BciVI	GTATCC	1 cut(s)	903
BclI	TGATCA	1 cut(s)	636
BcnI	CCSGG	8 cut(s)	29, 220, 232, 299, 897, 1131, 3001, 3002
BcoDI	GTCTC	6 cut(s)	55, 142, 618, 1740, 2044, 2490
BfaI	CTAG	3 cut(s)	801, 1818, 2064
BfmI	CTRYAG	1 cut(s)	1848
BfrI	CTTAAG	2 cut(s)	821, 842
BfuI	GTATCC	1 cut(s)	903
BglI	GCCNNNNNGGC	1 cut(s)	704
BglII	AGATCT	1 cut(s)	838
Bme18I	GGWCC	5 cut(s)	1079, 1295, 2399, 2571, 2780
BmeRI	GACNNNNNGTC	1 cut(s)	429
BmeT110I	CYCGRG	4 cut(s)	196, 1162, 1489, 3000
BmgT120I	GGNCC	6 cut(s)	1079, 1295, 2387, 2399, 2571, 2780
BmiI	GGNNCC	8 cut(s)	224, 691, 1297, 2400, 2427, 2500, 2572, 3032
BoxI	GACNNNNGTC	1 cut(s)	752
BpiI	GAAGAC	1 cut(s)	752
BpmI	CTGGAG	1 cut(s)	1977
Bpu10I	CCTNAGC	2 cut(s)	1248, 1860
BpuEI	CTTGAG	1 cut(s)	2725
BpuMI	CCSGG	8 cut(s)	29, 220, 232, 299, 897, 1131, 3001, 3002
Bsa29I	ATCGAT	2 cut(s)	377, 392
BsaHI	GRCGYC	1 cut(s)	360
BsaI	GGTCTC	1 cut(s)	55
BsaWI	WCCGGW	4 cut(s)	863, 2016, 2459, 3112
BsaXI	ACNNNNNCTCC	2 cut(s)	2319, 2349
Bse118I	RCCGGY	2 cut(s)	650, 2459
Bse1I	ACTGG	3 cut(s)	1805, 1918, 2798
Bse21I	CCTNAGG	1 cut(s)	2739
Bse3DI	GCAATG	3 cut(s)	714, 756, 2079
BseBI	CCWGG	8 cut(s)	584, 599, 1011, 1097, 2316, 2391, 2805, 3028
BseCI	ATCGAT	2 cut(s)	377, 392
BseGI	GGATG	8 cut(s)	501, 726, 853, 1128, 1540, 1660, 2438, 2443
BseMI	GCAATG	3 cut(s)	714, 756, 2079
BseMII	CTCAG	9 cut(s)	83, 579, 581, 894, 1262, 1851, 2501, 2730, 2912
BseNI	ACTGG	3 cut(s)	1805, 1918, 2798
BsePI	GCGCGC	1 cut(s)	7
BseRI	GAGGAG	4 cut(s)	88, 130, 317, 2940
BseSI	GKGCMC	2 cut(s)	372, 1907
BseYI	CCCAGC	3 cut(s)	1340, 1967, 2726
BsgI	GTGCAG	3 cut(s)	2496, 2511, 3085
Bsh1236I	CGCG	6 cut(s)	9, 11, 166, 206, 701, 3066
Bsh1285I	CGRYCG	1 cut(s)	236
BshNI	GGYRCC	1 cut(s)	2425
BshTI	ACCGGT	1 cut(s)	2459
BshVI	ATCGAT	2 cut(s)	377, 392
BsiEI	CGRYCG	1 cut(s)	236
BsiHKAI	GWGCWC	6 cut(s)	86, 98, 1496, 1649, 1907, 2950
BsiHKCI	CYCGRG	4 cut(s)	196, 1162, 1489, 3000
BslFI	GGGAC	7 cut(s)	193, 503, 913, 1232, 1562, 2459, 2592
BsmAI	GTCTC	6 cut(s)	55, 142, 618, 1740, 2044, 2490
BsmBI	CGTCTC	1 cut(s)	142
BsmFI	GGGAC	7 cut(s)	193, 503, 913, 1232, 1562, 2459, 2592
BsmI	GAATGC	5 cut(s)	673, 946, 2296, 2367, 2617
Bso31I	GGTCTC	1 cut(s)	55
BsoBI	CYCGRG	4 cut(s)	196, 1162, 1489, 3000
Bsp1286I	GDGCHC	8 cut(s)	86, 98, 372, 1496, 1649, 1907, 2525, 2950
Bsp19I	CCATGG	1 cut(s)	2776
BspCNI	CTCAG	9 cut(s)	84, 580, 580, 895, 1261, 1852, 2500, 2731, 2911
BspDI	ATCGAT	2 cut(s)	377, 392
BspFNI	CGCG	6 cut(s)	9, 11, 166, 206, 701, 3066
BspHI	TCATGA	2 cut(s)	2248, 2814
BspLI	GGNNCC	8 cut(s)	224, 691, 1297, 2400, 2427, 2500, 2572, 3032
BspPI	GGATC	3 cut(s)	1737, 1771, 2016
BspQI	GCTCTTC	1 cut(s)	2127
BspT107I	GGYRCC	1 cut(s)	2425
BspTI	CTTAAG	2 cut(s)	821, 842
BspTNI	GGTCTC	1 cut(s)	55
BsrDI	GCAATG	3 cut(s)	714, 756, 2079
BsrFI	RCCGGY	2 cut(s)	650, 2459
BsrI	ACTGG	3 cut(s)	1805, 1918, 2798
BssAI	RCCGGY	2 cut(s)	650, 2459
BssHII	GCGCGC	1 cut(s)	7
BssNAI	GTATAC	1 cut(s)	2269
BssNI	GRCGYC	1 cut(s)	360
BssT1I	CCWWGG	6 cut(s)	1527, 1758, 2112, 2516, 2574, 2776
Bst1107I	GTATAC	1 cut(s)	2269
Bst2UI	CCWGG	8 cut(s)	584, 599, 1011, 1097, 2316, 2391, 2805, 3028
Bst6I	CTCTTC	2 cut(s)	2127, 2883
BstACI	GRCGYC	1 cut(s)	360
BstAFI	CTTAAG	2 cut(s)	821, 842
BstAPI	GCANNNNNTGC	6 cut(s)	1239, 1259, 1598, 1696, 1717, 3143
BstC8I	GCNNGC	4 cut(s)	9, 21, 1173, 1617
BstDEI	CTNAG	9 cut(s)	92, 567, 588, 903, 1248, 1860, 2487, 2739, 2898
BstDSI	CCRYGG	4 cut(s)	243, 699, 2662, 2776
BstEII	GGTNACC	2 cut(s)	1892, 3083
BstENI	CCTNNNNNAGG	1 cut(s)	3057
BstF5I	GGATG	8 cut(s)	501, 726, 853, 1128, 1540, 1660, 2438, 2443
BstFNI	CGCG	6 cut(s)	9, 11, 166, 206, 701, 3066
BstHHI	GCGC	6 cut(s)	9, 11, 168, 1048, 1177, 1603
BstMAI	GTCTC	6 cut(s)	55, 142, 618, 1740, 2044, 2490
BstMCI	CGRYCG	1 cut(s)	236
BstNI	CCWGG	8 cut(s)	584, 599, 1011, 1097, 2316, 2391, 2805, 3028
BstNSI	RCATGY	3 cut(s)	518, 1439, 1711
BstPAI	GACNNNNGTC	1 cut(s)	752
BstPI	GGTNACC	2 cut(s)	1892, 3083
BstSFI	CTRYAG	1 cut(s)	1848
BstSLI	GKGCMC	2 cut(s)	372, 1907
BstUI	CGCG	6 cut(s)	9, 11, 166, 206, 701, 3066
BstV2I	GAAGAC	1 cut(s)	752
BstX2I	RGATCY	3 cut(s)	838, 1729, 1763
BstXI	CCANNNNNNTGG	2 cut(s)	1812, 2635
BstYI	RGATCY	3 cut(s)	838, 1729, 1763
BstZ17I	GTATAC	1 cut(s)	2269
Bsu15I	ATCGAT	2 cut(s)	377, 392
Bsu36I	CCTNAGG	1 cut(s)	2739
BsuI	GTATCC	1 cut(s)	903
BsuTUI	ATCGAT	2 cut(s)	377, 392
BtgI	CCRYGG	4 cut(s)	243, 699, 2662, 2776
BtgZI	GCGATG	1 cut(s)	728
BtsCI	GGATG	8 cut(s)	501, 726, 853, 1128, 1540, 1660, 2438, 2443
BtsI	GCAGTG	6 cut(s)	674, 1242, 1584, 2963, 3126, 3141
Cac8I	GCNNGC	4 cut(s)	9, 21, 1173, 1617
CaiI	CAGNNNCTG	2 cut(s)	2804, 3143
CciI	TCATGA	2 cut(s)	2248, 2814
CfoI	GCGC	6 cut(s)	9, 11, 168, 1048, 1177, 1603
Cfr10I	RCCGGY	2 cut(s)	650, 2459
Cfr13I	GGNCC	6 cut(s)	1079, 1295, 2387, 2399, 2571, 2780
Cfr42I	CCGCGG	1 cut(s)	702
Cfr9I	CCCGGG	1 cut(s)	3000
ClaI	ATCGAT	2 cut(s)	377, 392
CseI	GACGC	6 cut(s)	195, 257, 368, 1439, 2744, 3072
Csp6I	GTAC	7 cut(s)	133, 562, 571, 1114, 1279, 1770, 2052
CspAI	ACCGGT	1 cut(s)	2459
CviQI	GTAC	7 cut(s)	133, 562, 571, 1114, 1279, 1770, 2052
DdeI	CTNAG	9 cut(s)	92, 567, 588, 903, 1248, 1860, 2487, 2739, 2898
DraIII	CACNNNGTG	1 cut(s)	2209
DriI	GACNNNNNGTC	1 cut(s)	429
EaeI	YGGCCR	3 cut(s)	1098, 2625, 2795
Eam1104I	CTCTTC	2 cut(s)	2127, 2883
Eam1105I	GACNNNNNGTC	1 cut(s)	429
EarI	CTCTTC	2 cut(s)	2127, 2883
EciI	GGCGGA	4 cut(s)	59, 280, 335, 1040
Ecl136II	GAGCTC	3 cut(s)	84, 96, 2948
Eco130I	CCWWGG	6 cut(s)	1527, 1758, 2112, 2516, 2574, 2776
Eco147I	AGGCCT	1 cut(s)	1305
Eco24I	GRGCYC	4 cut(s)	86, 98, 2525, 2950
Eco31I	GGTCTC	1 cut(s)	55
Eco47I	GGWCC	5 cut(s)	1079, 1295, 2399, 2571, 2780
Eco53kI	GAGCTC	3 cut(s)	84, 96, 2948
Eco57I	CTGAAG	2 cut(s)	2159, 2172
Eco81I	CCTNAGG	1 cut(s)	2739
Eco88I	CYCGRG	4 cut(s)	196, 1162, 1489, 3000
Eco91I	GGTNACC	2 cut(s)	1892, 3083
EcoICRI	GAGCTC	3 cut(s)	84, 96, 2948
EcoNI	CCTNNNNNAGG	1 cut(s)	3057
EcoO109I	RGGNCCY	2 cut(s)	1079, 2399
EcoO65I	GGTNACC	2 cut(s)	1892, 3083
EcoRI	GAATTC	1 cut(s)	2690
EcoRII	CCWGG	8 cut(s)	582, 597, 1009, 1095, 2314, 2389, 2803, 3026
EcoT14I	CCWWGG	6 cut(s)	1527, 1758, 2112, 2516, 2574, 2776
EcoT22I	ATGCAT	4 cut(s)	673, 1713, 2593, 2983
EcoT38I	GRGCYC	4 cut(s)	86, 98, 2525, 2950
ErhI	CCWWGG	6 cut(s)	1527, 1758, 2112, 2516, 2574, 2776
Esp3I	CGTCTC	1 cut(s)	142
FalI	AAGNNNNNCTT	2 cut(s)	2405, 2437
FaqI	GGGAC	7 cut(s)	193, 503, 913, 1232, 1562, 2459, 2592
FauI	CCCGC	4 cut(s)	284, 323, 2964, 3028
FbaI	TGATCA	1 cut(s)	636
FblI	GTMKAC	2 cut(s)	1485, 2268
FokI	GGATG	8 cut(s)	508, 713, 860, 1135, 1547, 1647, 2425, 2430
FriOI	GRGCYC	4 cut(s)	86, 98, 2525, 2950
FspBI	CTAG	3 cut(s)	801, 1818, 2064
FspI	TGCGCA	1 cut(s)	1047
GlaI	GCGC	6 cut(s)	8, 10, 167, 1047, 1176, 1602
GsaI	CCCAGC	3 cut(s)	1344, 1971, 2730
GsuI	CTGGAG	1 cut(s)	1977
HgaI	GACGC	6 cut(s)	195, 257, 368, 1439, 2744, 3072
HhaI	GCGC	6 cut(s)	9, 11, 168, 1048, 1177, 1603
Hin1I	GRCGYC	1 cut(s)	360
Hin6I	GCGC	6 cut(s)	7, 9, 166, 1046, 1175, 1601
HinP1I	GCGC	6 cut(s)	7, 9, 166, 1046, 1175, 1601
HincII	GTYRAC	6 cut(s)	423, 993, 1540, 1635, 2701, 3070
HindII	GTYRAC	6 cut(s)	423, 993, 1540, 1635, 2701, 3070
HindIII	AAGCTT	3 cut(s)	1460, 1544, 1554
HpaI	GTTAAC	1 cut(s)	1635
HphI	GGTGA	9 cut(s)	640, 878, 1025, 1816, 1826, 1904, 2197, 2945, 3095
Hpy188III	TCNNGA	7 cut(s)	37, 198, 481, 1213, 1871, 2249, 2815
Hpy99I	CGWCG	2 cut(s)	130, 362
HpyCH4IV	ACGT	3 cut(s)	135, 2334, 2745
HpyF3I	CTNAG	9 cut(s)	92, 567, 588, 903, 1248, 1860, 2487, 2739, 2898
HpySE526I	ACGT	3 cut(s)	135, 2334, 2745
Hsp92I	GRCGYC	1 cut(s)	360
HspAI	GCGC	6 cut(s)	7, 9, 166, 1046, 1175, 1601
Ksp22I	TGATCA	1 cut(s)	636
KspAI	GTTAAC	1 cut(s)	1635
KspI	CCGCGG	1 cut(s)	702
LguI	GCTCTTC	1 cut(s)	2127
MaeI	CTAG	3 cut(s)	801, 1818, 2064
MaeII	ACGT	3 cut(s)	135, 2334, 2745
MaeIII	GTNAC	8 cut(s)	719, 829, 1807, 1892, 2092, 2185, 3083, 3089
MflI	RGATCY	3 cut(s)	838, 1729, 1763
MhlI	GDGCHC	8 cut(s)	86, 98, 372, 1496, 1649, 1907, 2525, 2950
MlsI	TGGCCA	3 cut(s)	1100, 2627, 2797
MluI	ACGCGT	2 cut(s)	204, 3064
MluNI	TGGCCA	3 cut(s)	1100, 2627, 2797
MlyI	GAGTC	8 cut(s)	179, 341, 378, 439, 620, 1481, 1916, 2574
MmeI	TCCRAC	3 cut(s)	588, 1336, 2300
Mox20I	TGGCCA	3 cut(s)	1100, 2627, 2797
Mph1103I	ATGCAT	4 cut(s)	673, 1713, 2593, 2983
MscI	TGGCCA	3 cut(s)	1100, 2627, 2797
MslI	CAYNNNNRTG	5 cut(s)	951, 2019, 2379, 2561, 2813
Msp20I	TGGCCA	3 cut(s)	1100, 2627, 2797
MspA1I	CMGCKG	1 cut(s)	701
MspCI	CTTAAG	2 cut(s)	821, 842
Mva1269I	GAATGC	5 cut(s)	673, 946, 2296, 2367, 2617
MvaI	CCWGG	8 cut(s)	584, 599, 1011, 1097, 2316, 2391, 2805, 3028
MvnI	CGCG	6 cut(s)	9, 11, 166, 206, 701, 3066
NciI	CCSGG	8 cut(s)	29, 220, 232, 299, 897, 1131, 3001, 3002
NcoI	CCATGG	1 cut(s)	2776
NlaIV	GGNNCC	8 cut(s)	224, 691, 1297, 2400, 2427, 2500, 2572, 3032
NmeAIII	GCCGAG	2 cut(s)	2021, 2872
NmuCI	GTSAC	4 cut(s)	1807, 1892, 2185, 3083
NsbI	TGCGCA	1 cut(s)	1047
NsiI	ATGCAT	4 cut(s)	673, 1713, 2593, 2983
NspI	RCATGY	3 cut(s)	518, 1439, 1711
OliI	CACNNNNGTG	1 cut(s)	2379
PaeR7I	CTCGAG	2 cut(s)	196, 1489
PagI	TCATGA	2 cut(s)	2248, 2814
PasI	CCCWGGG	1 cut(s)	598
PauI	GCGCGC	1 cut(s)	7
PceI	AGGCCT	1 cut(s)	1305
PciI	ACATGT	1 cut(s)	514
PciSI	GCTCTTC	1 cut(s)	2127
PcsI	WCGNNNNNNNCGW	4 cut(s)	194, 203, 342, 363
PctI	GAATGC	5 cut(s)	673, 946, 2296, 2367, 2617
PfeI	GAWTC	6 cut(s)	172, 379, 389, 1157, 1753, 2056
PflMI	CCANNNNNTGG	3 cut(s)	2449, 2804, 3048
PinAI	ACCGGT	1 cut(s)	2459
PleI	GAGTC	8 cut(s)	179, 341, 378, 438, 619, 1481, 1916, 2574
PpsI	GAGTC	8 cut(s)	179, 341, 378, 438, 619, 1481, 1916, 2574
PpuMI	RGGWCCY	2 cut(s)	1079, 2399
PscI	ACATGT	1 cut(s)	514
PshAI	GACNNNNGTC	1 cut(s)	752
Psp124BI	GAGCTC	3 cut(s)	86, 98, 2950
Psp5II	RGGWCCY	2 cut(s)	1079, 2399
Psp6I	CCWGG	8 cut(s)	582, 597, 1009, 1095, 2314, 2389, 2803, 3026
PspEI	GGTNACC	2 cut(s)	1892, 3083
PspFI	CCCAGC	3 cut(s)	1340, 1967, 2726
PspGI	CCWGG	8 cut(s)	582, 597, 1009, 1095, 2314, 2389, 2803, 3026
PspN4I	GGNNCC	8 cut(s)	224, 691, 1297, 2400, 2427, 2500, 2572, 3032
PspPI	GGNCC	6 cut(s)	1079, 1295, 2387, 2399, 2571, 2780
PspPPI	RGGWCCY	2 cut(s)	1079, 2399
PspXI	VCTCGAGB	1 cut(s)	1489
PstNI	CAGNNNCTG	2 cut(s)	2804, 3143
PsuI	RGATCY	3 cut(s)	838, 1729, 1763
PteI	GCGCGC	1 cut(s)	7
RsaI	GTAC	7 cut(s)	134, 563, 572, 1115, 1280, 1771, 2053
RsaNI	GTAC	7 cut(s)	133, 562, 571, 1114, 1279, 1770, 2052
RseI	CAYNNNNRTG	5 cut(s)	951, 2019, 2379, 2561, 2813
SacI	GAGCTC	3 cut(s)	86, 98, 2950
SacII	CCGCGG	1 cut(s)	702
SapI	GCTCTTC	1 cut(s)	2127
Sau96I	GGNCC	6 cut(s)	1079, 1295, 2387, 2399, 2571, 2780
SchI	GAGTC	8 cut(s)	179, 341, 378, 439, 620, 1481, 1916, 2574
SduI	GDGCHC	8 cut(s)	86, 98, 372, 1496, 1649, 1907, 2525, 2950
SfcI	CTRYAG	1 cut(s)	1848
Sfr274I	CTCGAG	2 cut(s)	196, 1489
Sfr303I	CCGCGG	1 cut(s)	702
SgrBI	CCGCGG	1 cut(s)	702
SinI	GGWCC	5 cut(s)	1079, 1295, 2399, 2571, 2780
SlaI	CTCGAG	2 cut(s)	196, 1489
SmaI	CCCGGG	1 cut(s)	3002
SmiMI	CAYNNNNRTG	5 cut(s)	951, 2019, 2379, 2561, 2813
SmlI	CTYRAG	5 cut(s)	196, 821, 842, 1489, 2704
SmoI	CTYRAG	5 cut(s)	196, 821, 842, 1489, 2704
SseBI	AGGCCT	1 cut(s)	1305
SspI	AATATT	1 cut(s)	541
SspMI	CTAG	3 cut(s)	801, 1818, 2064
SstI	GAGCTC	3 cut(s)	86, 98, 2950
StuI	AGGCCT	1 cut(s)	1305
StyI	CCWWGG	6 cut(s)	1527, 1758, 2112, 2516, 2574, 2776
TaiI	ACGT	3 cut(s)	138, 2337, 2748
TatI	WGTACW	2 cut(s)	570, 1113
TauI	GCSGC	5 cut(s)	19, 282, 701, 1009, 1091
TfiI	GAWTC	6 cut(s)	172, 379, 389, 1157, 1753, 2056
TseFI	GTSAC	4 cut(s)	1807, 1892, 2185, 3083
Tsp45I	GTSAC	4 cut(s)	1807, 1892, 2185, 3083
TspDTI	ATGAA	9 cut(s)	410, 464, 1319, 1790, 2099, 2229, 2237, 2303, 2683
TspGWI	ACGGA	5 cut(s)	232, 2220, 2391, 2651, 3023
TspMI	CCCGGG	1 cut(s)	3000
Van91I	CCANNNNNTGG	3 cut(s)	2449, 2804, 3048
Vha464I	CTTAAG	2 cut(s)	821, 842
VneI	GTGCAC	1 cut(s)	1903
VpaK11BI	GGWCC	5 cut(s)	1079, 1295, 2399, 2571, 2780
XagI	CCTNNNNNAGG	1 cut(s)	3057
XapI	RAATTY	4 cut(s)	407, 454, 1471, 2690
XceI	RCATGY	3 cut(s)	518, 1439, 1711
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2758, 2790
XhoI	CTCGAG	2 cut(s)	196, 1489
XmaI	CCCGGG	1 cut(s)	3000
XmiI	GTMKAC	2 cut(s)	1485, 2268
XspI	CTAG	3 cut(s)	801, 1818, 2064
Zsp2I	ATGCAT	4 cut(s)	673, 1713, 2593, 2983

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.