Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0006217

Physical Location & Seq

Genomic Coordinates Forward (+)

52956513 .. 52963513

Gene Structure 7001 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0383900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3276 bp

ATGGCGACGCCATCGGCGACAACGACAACACTGACTAAAGACCTCACTTTCTTGATCATGCAATTCCTTGACGAGGACAAGTGGAAGGATACGCTTCATAAGCTTCAGCAAGAATCAGGATACTTCTTCGACATGAAGTATTTCGAGGAATTATTGCTCGAGGGAAATTGGAATGAAGTCGAGAAGTATCTTTCCGGGTTCACTAAAGTGGATGACAACCGCTATTCGATGAAGATATTCTTCGAAATTCGTAAGCAGAAATACTTAGAAGCGCTGGATAGACATGATAGAGCTGCCGCAGTAGATATCCTAGTGAAGGATCTGAAAGTCTTTGCAGGTTTTAACGAAGACTTGTACAAGGAAATCACTCAGCTGCTCACTTTGAACAATTTTAGAGACAATGCTCAACTCTCTGCTTATGCAGATATAAGGACTGCAAGAGCAATTATGGCCCAAGAACTTAAGAAGCTTGTTGAGGCCAACCCTTCTTTCCGTGAAAAATTACAGTTCCCTAGCATTAAAAGTTCAAGGCTACGGACGCTTATTAACCAAAGCTTAAATTGGCAGCATTCCCTTTGTACTAATCCTAAACAAAATCCGGAAATAAAAACCCTGTTTGTGGATCACACTTGTAGACATTCCAACGATCCATTTGCTCATTTAGCAGCAAATGGTCAGGCTCAACAGATGGGAGCAGTACAGAGAGCTGATGGCTTCATTCCTATCGGTGTAAATGGGTCCTTCCAACCCCCAATAGCACCAGTACCAATTCCACCTCCTTTGTCAACATGGACGTCCATACCCCCCAGTGTCAATCAGTTGGCAGCTTCTGCAGGAGGTATTGGTTTTGGCAGTCCAGCAAATCCAGCTTCATATTTGAACAGTCCAGCAAATCCTGAAGATTTGTTCAGAACACGGTTCCCTGGGGTTTCAGACAGGGTTATGCTGCCAGGGGTTAATCCAGGCCACGTTCTAAGTCCAGCATTCAATGCAATTGATGAGTTTCCGAAGGCTGTTGCCCGGATACTGAATCAGGGCTCAGTTCCCACTAGCATGGACTTCCATCCTCTTCAACAGACTATACTTCTAGTTGGAACTAGTATGGGGGATATAAGTGTCTGGGAAGTCAGTTCTAGGGAGAAGTTGGTATCAAGAAGCTTTCAGCCATGGGATATGGGGGCAAGTTCAATGATACTAAAGGCAACTTTAGTCAAGGATCCAAGTGTCTCAGTTAAGCGTGTACTATGGAGCCCTGATGGTTCTCTATTCGGAGTTGCATATTCTAAACACATGATGCAGTTATATGGTTATTTTGGTGGTGATGATGTTCGTCAGCATTTAGAGATCGATGCTCATGTTGGTAGTGTAAATGATCTGGCGTTCTGCAACCCAACTAAGCAACTCTGTGTCATAACTTGCGGTGATGATAAGGCCATCAAGGTCTGGGATGTGGCTACTGGATCAAAATTGTATACTTTTGAAGGTCATGAGGCTCCTGTTCATTCTGTGTGCCCTCATAGCAAAGAACATGTCCATTTTGTCTTTTCGACATCAGTAGATGGACAAATAAAAGCATGGTTATATGAAGTGGTGGGGCCCAGGGTTAATTACGATTCTCCCAGCCTCTCATGCACAACGATGGTTTATAGTGCAGATGGTAAAAGGCTTTTTTCATGTGGGACGAGCAAAGATGGAGAATCTCATGTTGTTGAATGGAATGAAAATGAAGGCACAATAAAGAGGAATTACCAAGGATTTCAAAAACAATCTTTGGGTGTTGTTCAATTTGACACAACCAGGAATAAGTATCTAGCAGTTGGTGACGACTATGCAATCAAAGTTTGGGATATGGATAATATCAATCTTTTGGCTACTATTGATGCTGAAGGAGGTCTACCAGCAACCCCTCGTATCCGCTTCAACAAGGAAGGATCTTTGTTAGCTGTTTCTGCAAATGACAACAGAATCAAAGTTTTGGCTACAACAGATGGTCTCCGATTGATGCGCAATTATGAAAGTCTCTCGCTTATTGCCTCTAGAAATGCACCAGAAAAGAATGGTAGCACCAGAAGCATGGAGGATAATAAGAAACCAAAGTTAACTGAAGAGCTCAAACCTGCAAAGATCTGGAAGCCCACTGAGATTAGTGAAACTGCTCGGCTTCGGTCTCTAAGGCTCTCGACAACTACAGTTAAAACAGACAAGATCTCAAGGTTGGTTTACACTAATTCAGGTAATGCCATTCTGGCATTATCATCAAATGGAACCCATCTACTGTGGAGGTGGCCACAAGCTGACCGTAGTACGATTGGAAAGGCAACAACCAATGTTACCCCAAAATTCGTGGAACAAGCTTCAGGCATCCTGATGATCAATGATCTTACTGGTGCTAAACCTGAAGATGCAATACCTTGCTTCGCTTTGTCCAAGAATGATTCGTATGTCATGTCAGCCTCGGGAGGGAAGATATCGTTGTTCAACCTAATGACATTCAAGACGATGACAAATTTCATGTCGCCACCACCTGTGGTAACATGTGTTGCTTTCCACCCTGAAGACAATAATATAGTTGCTGTTGGATTTGATGATTCGACTATTCACATATACAATGTCCGTATCGATGAGGTTAGGATAAAGCTCAAGGGTCACTCTAAGAGAGTCACAGGCCTTGCGTTCTCTCAACTTCTGAAGACACTTGTTTCTTCTGGAGCAGATGCTCAGATAGTTGTGTGGAGCTCTGATAAGTGGGAAAGGCAGAAGAACAGTTTCTTGCAGGTTCCAGCTTGGACAACACCCACAGCACTGTTTGGAACTCATCTTCAGTATCAGAAGGATCAGAATCACTTCCTCGTTGTACACGAGACTCAACTTGCTGTATACGAAACCTTGAGGCTTGAATGCCAGAAGAAGTGGGTAGTAGGAGAATCTTCAGCACCAATCTCCTATGCAACATTCTCTTGTGATAGCCAGTTAGTCTACGCGAGCTTTCTTGATGGAACTGTGCGCGTGTTTGCAGCATCGAATCTTCAAATGCAATGCCAGATTAACCCCTATGCTTATCTTCCGCCTAATGTCAGCTCTGCAACATACCCCCTAGTGATAGCTGCGAATCCACACGAGCCGAACCAGTTTGCAGTGGGACTAACAGATGGTGCAGTTGTCATGTTTGAACCACTTGAGTCTGGAGACAAATGGGGTGTGCCGCCACCAGCAGACAATGGTTGCTTCCATGCTTCAACTTCTGCCAATGGTTCCAGTTTAGAACTCCAGAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001505 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006520 GO:0006544 GO:0006546 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009069 GO:0009071 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0016054 GO:0017076 GO:0017144 GO:0019752 GO:0030554 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042133 GO:0042135 GO:0042221 GO:0042737 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046395 GO:0046686 GO:0048046 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606

KEGG Pathways

Metabolic & Signaling

ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200

Pfam Domains

Protein Families

GDC-P

Protein Analysis

1091

Amino Acids

120.89

Weight (kDa)

6.62

Isoelectric Point (pI)

35.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	13 - 40	3.8e-09	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	44 - 168	1.1e-58	TPR1-like, CTLH-containing domain
zf_topless	PF21359	171 - 211	5e-25	TOPLESS, zinc finger domain
Beta-prop_WDR3_1st	PF25173	390 - 558	3.3e-15	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	405 - 560	1e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	410 - 526	2.4e-13	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	410 - 667	5.6e-24	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	417 - 526	2.4e-08	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	426 - 528	3.3e-10	WDHD1 first WD40 domain
Beta-prop_EML	PF23409	448 - 629	2.1e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	449 - 659	5.9e-16	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	461 - 528	4e-07	TEP-1 second beta-propeller
Beta-prop_EML_2	PF23414	462 - 621	4.7e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	470 - 561	8.6e-06	WDR90/POC16, second beta-propeller
Beta-prop_WDR5	PF25175	512 - 665	5.3e-09	WDR5 beta-propeller domain
WDR55	PF24796	515 - 658	7.3e-06	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	566 - 671	9.9e-06	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	727 - 998	1.8e-15	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	798 - 918	2.4e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	800 - 915	6.1e-15	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	800 - 940	1.6e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	801 - 1008	3.6e-19	WDR5 beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	802 - 1034	5.6e-08	MABP1/WDR62 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	805 - 1012	3e-12	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	808 - 1008	3.6e-18	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	809 - 912	1.2e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	814 - 912	2.3e-16	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	816 - 912	1.5e-10	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	816 - 924	7e-09	IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	821 - 915	3.9e-10	WDR90/POC16, second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	855 - 911	1e-05	MABP1/WDR62 second WD40 domain
Beta-prop_THOC3	PF25174	855 - 1015	2.2e-08	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11850
fragaria_vesca	FvH4_2g31950 FvH4_2g31950 FvH4_2g31950 FvH4_2g31950 FvH4_2g31950 FvH4_2g31950 FvH4_2g31950 FvH4_2g31950 FvH4_2g31950
malus_domestica	MD08G1061200.v1.1 MD15G1060300.v1.1
prunus_persica	Prupe.1G412200_v2.0.a1
pyrus_communis	pycom02g10370 pycom08g05080 pycom12g06840 pycom15g05670
rosa_chinensis	RchiOBHm_Chr6g0310891
rosa_laevigata	RLG00000010453
rosa_multiflora	Rmu_ssc0000395.1_g000002
rosa_roxburghii	Rroxscaffold_7G00158210
rosa_rugosa	Rorug06G0383900
rosa_samantha	Rh6AG497600 Rh6BG507700 Rh6CG513700 Rh6DG500300
rosa_wichuraiana	Rw6G043340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	797
Acc16I	TGCGCA	1 cut(s)	2006
Acc36I	ACCTGC	3 cut(s)	326, 2125, 2764
AccI	GTMKAC	5 cut(s)	634, 1472, 1894, 2877, 2976
AccII	CGCG	2 cut(s)	2981, 3006
AccIII	TCCGGA	1 cut(s)	598
AciI	CCGC	6 cut(s)	220, 297, 1419, 1915, 3065, 3203
AclWI	GGATC	8 cut(s)	327, 630, 641, 1211, 1224, 1468, 1939, 2841
AcoI	YGGCCR	1 cut(s)	2285
AcsI	RAATTY	3 cut(s)	246, 2339, 2506
AcyI	GRCGYC	2 cut(s)	8, 794
AfaI	GTAC	7 cut(s)	356, 580, 699, 765, 1242, 2305, 2856
AfeI	AGCGCT	1 cut(s)	273
AfiI	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2460
AflII	CTTAAG	1 cut(s)	461
AflIII	ACRYGT	2 cut(s)	1528, 2534
AhlI	ACTAGT	1 cut(s)	1097
AjnI	CCWGG	5 cut(s)	922, 949, 961, 1598, 1796
AjuI	GAANNNNNNNTTGG	2 cut(s)	473, 505
AleI	CACNNNNGTG	1 cut(s)	206
Alw21I	GWGCWC	2 cut(s)	2112, 2738
Alw26I	GTCTC	7 cut(s)	390, 1231, 1997, 2024, 2172, 2855, 3180
AlwI	GGATC	8 cut(s)	327, 630, 641, 1211, 1224, 1468, 1939, 2841
AlwNI	CAGNNNCTG	1 cut(s)	830
Ama87I	CYCGRG	2 cut(s)	158, 2455
Aor13HI	TCCGGA	1 cut(s)	598
Aor51HI	AGCGCT	1 cut(s)	273
AoxI	GGCC	7 cut(s)	450, 477, 964, 1431, 1595, 2285, 2665
ApaI	GGGCCC	1 cut(s)	1599
ApeKI	GCWGC	8 cut(s)	293, 373, 565, 665, 824, 946, 3014, 3104
ApoI	RAATTY	3 cut(s)	246, 2339, 2506
ArsI	GACNNNNNNTTYG	4 cut(s)	1515, 1547, 2498, 2530
Asp700I	GAANNNNTTC	4 cut(s)	140, 236, 2765, 2843
AspLEI	GCGC	3 cut(s)	274, 2007, 3006
AspS9I	GGNCC	4 cut(s)	451, 738, 1595, 1596
AsuC2I	CCSGG	2 cut(s)	196, 1021
AsuHPI	GGTGA	3 cut(s)	1331, 1433, 1832
AsuII	TTCGAA	1 cut(s)	243
AvaI	CYCGRG	2 cut(s)	158, 2455
AvaII	GGWCC	1 cut(s)	738
BaeGI	GKGCMC	2 cut(s)	1514, 1599
BalI	TGGCCA	1 cut(s)	2287
BamHI	GGATCC	1 cut(s)	1216
BanII	GRGCYC	5 cut(s)	1040, 1253, 1599, 2112, 2738
BarI	GAAGNNNNNNTAC	4 cut(s)	1878, 1910, 2391, 2423
BauI	CACGAG	2 cut(s)	2858, 3116
BbsI	GAAGAC	3 cut(s)	354, 2562, 2696
Bbv12I	GWGCWC	2 cut(s)	2112, 2738
BbvI	GCAGC	8 cut(s)	280, 360, 577, 677, 836, 933, 3026, 3091
BcgI	CGANNNNNNTGC	2 cut(s)	635, 669
BciT130I	CCWGG	5 cut(s)	924, 951, 963, 1600, 1798
BciVI	GTATCC	4 cut(s)	82, 113, 1017, 1922
BclI	TGATCA	2 cut(s)	54, 2370
BcnI	CCSGG	2 cut(s)	196, 1021
BcoDI	GTCTC	7 cut(s)	390, 1231, 1997, 2024, 2172, 2855, 3180
BcuI	ACTAGT	1 cut(s)	1097
BfaI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 2037, 3095
BfmI	CTRYAG	2 cut(s)	831, 2187
BfoI	RGCGCY	1 cut(s)	275
BfrI	CTTAAG	1 cut(s)	461
BfuAI	ACCTGC	3 cut(s)	326, 2125, 2764
BfuI	GTATCC	4 cut(s)	82, 113, 1017, 1922
BglI	GCCNNNNNGGC	1 cut(s)	2246
BglII	AGATCT	2 cut(s)	2124, 2205
Bme1390I	CCNGG	7 cut(s)	196, 924, 951, 963, 1021, 1600, 1798
Bme18I	GGWCC	1 cut(s)	738
BmeT110I	CYCGRG	2 cut(s)	158, 2455
BmgT120I	GGNCC	4 cut(s)	451, 738, 1595, 1596
BmrFI	CCNGG	7 cut(s)	196, 924, 951, 963, 1021, 1600, 1798
BmrI	ACTGGG	1 cut(s)	801
BmsI	GCATC	8 cut(s)	1284, 1339, 1870, 1992, 2370, 2392, 2704, 3026
BmuI	ACTGGG	1 cut(s)	801
BpiI	GAAGAC	3 cut(s)	354, 2562, 2696
BpmI	CTGGAG	3 cut(s)	2727, 3204, 3251
Bpu14I	TTCGAA	1 cut(s)	243
BpuEI	CTTGAG	4 cut(s)	2194, 2624, 2908, 3197
BpuMI	CCSGG	2 cut(s)	196, 1021
Bsa29I	ATCGAT	2 cut(s)	1347, 2619
BsaBI	GATNNNNATC	1 cut(s)	2838
BsaHI	GRCGYC	2 cut(s)	8, 794
BsaI	GGTCTC	2 cut(s)	1997, 2172
BsaJI	CCNNGG	8 cut(s)	922, 923, 950, 1166, 1598, 1599, 1750, 2454
BsaWI	WCCGGW	1 cut(s)	598
Bsc4I	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2460
Bse1I	ACTGG	7 cut(s)	761, 807, 1462, 2389, 2968, 3127, 3255
Bse3DI	GCAATG	1 cut(s)	3041
Bse8I	GATNNNNATC	1 cut(s)	2838
BseAI	TCCGGA	1 cut(s)	598
BseBI	CCWGG	5 cut(s)	924, 951, 963, 1600, 1798
BseCI	ATCGAT	2 cut(s)	1347, 2619
BseDI	CCNNGG	8 cut(s)	922, 923, 950, 1166, 1598, 1599, 1750, 2454
BseGI	GGATG	4 cut(s)	217, 1063, 1453, 2361
BseJI	GATNNNNATC	1 cut(s)	2838
BseLI	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2460
BseMI	GCAATG	1 cut(s)	3041
BseMII	CTCAG	5 cut(s)	383, 1053, 1242, 2130, 2732
BseNI	ACTGG	7 cut(s)	761, 807, 1462, 2389, 2968, 3127, 3255
BseSI	GKGCMC	2 cut(s)	1514, 1599
BseXI	GCAGC	8 cut(s)	280, 360, 577, 677, 836, 933, 3026, 3091
BseYI	CCCAGC	1 cut(s)	1619
BsgI	GTGCAG	2 cut(s)	1671, 3174
Bsh1236I	CGCG	2 cut(s)	2981, 3006
BshFI	GGCC	7 cut(s)	452, 479, 966, 1433, 1597, 2287, 2667
BshVI	ATCGAT	2 cut(s)	1347, 2619
BsiHKAI	GWGCWC	2 cut(s)	2112, 2738
BsiHKCI	CYCGRG	2 cut(s)	158, 2455
BsiSI	CCGG	3 cut(s)	195, 599, 1021
BslFI	GGGAC	2 cut(s)	1693, 3153
BslI	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2460
BsmAI	GTCTC	7 cut(s)	390, 1231, 1997, 2024, 2172, 2855, 3180
BsmFI	GGGAC	2 cut(s)	1693, 3153
BsmI	GAATGC	3 cut(s)	568, 983, 2903
BsnI	GGCC	7 cut(s)	452, 479, 966, 1433, 1597, 2287, 2667
Bso31I	GGTCTC	2 cut(s)	1997, 2172
BsoBI	CYCGRG	2 cut(s)	158, 2455
Bsp119I	TTCGAA	1 cut(s)	243
Bsp120I	GGGCCC	1 cut(s)	1595
Bsp1286I	GDGCHC	6 cut(s)	1040, 1253, 1514, 1599, 2112, 2738
Bsp13I	TCCGGA	1 cut(s)	598
Bsp1407I	TGTACA	2 cut(s)	354, 2854
Bsp19I	CCATGG	1 cut(s)	1166
BspACI	CCGC	6 cut(s)	220, 297, 1419, 1915, 3065, 3203
BspANI	GGCC	7 cut(s)	452, 479, 966, 1433, 1597, 2287, 2667
BspCNI	CTCAG	5 cut(s)	382, 1052, 1241, 2131, 2731
BspDI	ATCGAT	2 cut(s)	1347, 2619
BspEI	TCCGGA	1 cut(s)	598
BspFNI	CGCG	2 cut(s)	2981, 3006
BspHI	TCATGA	1 cut(s)	1486
BspMAI	CTGCAG	1 cut(s)	835
BspMI	ACCTGC	3 cut(s)	326, 2125, 2764
BspPI	GGATC	8 cut(s)	327, 630, 641, 1211, 1224, 1468, 1939, 2841
BspQI	GCTCTTC	1 cut(s)	2100
BspT104I	TTCGAA	1 cut(s)	243
BspTI	CTTAAG	1 cut(s)	461
BspTNI	GGTCTC	2 cut(s)	1997, 2172
BsrDI	GCAATG	1 cut(s)	3041
BsrGI	TGTACA	2 cut(s)	354, 2854
BsrI	ACTGG	7 cut(s)	761, 807, 1462, 2389, 2968, 3127, 3255
BssECI	CCNNGG	8 cut(s)	922, 923, 950, 1166, 1598, 1599, 1750, 2454
BssNAI	GTATAC	2 cut(s)	1473, 2878
BssNI	GRCGYC	2 cut(s)	8, 794
BssSI	CACGAG	2 cut(s)	2858, 3116
BssT1I	CCWWGG	2 cut(s)	1166, 1750
Bst1107I	GTATAC	2 cut(s)	1473, 2878
Bst2BI	CACGAG	2 cut(s)	2858, 3116
Bst2UI	CCWGG	5 cut(s)	924, 951, 963, 1600, 1798
Bst4CI	ACNGT	9 cut(s)	507, 884, 918, 2191, 2277, 2300, 2765, 2805, 3001
Bst6I	CTCTTC	2 cut(s)	1074, 2100
BstACI	GRCGYC	2 cut(s)	8, 794
BstAFI	CTTAAG	1 cut(s)	461
BstAPI	GCANNNNNTGC	2 cut(s)	830, 989
BstAUI	TGTACA	2 cut(s)	354, 2854
BstBI	TTCGAA	1 cut(s)	243
BstC8I	GCNNGC	1 cut(s)	2983
BstDSI	CCRYGG	1 cut(s)	1166
BstENI	CCTNNNNNAGG	2 cut(s)	71, 314
BstF5I	GGATG	4 cut(s)	217, 1063, 1453, 2361
BstFNI	CGCG	2 cut(s)	2981, 3006
BstH2I	RGCGCY	1 cut(s)	275
BstHHI	GCGC	3 cut(s)	274, 2007, 3006
BstMAI	GTCTC	7 cut(s)	390, 1231, 1997, 2024, 2172, 2855, 3180
BstNI	CCWGG	5 cut(s)	924, 951, 963, 1600, 1798
BstNSI	RCATGY	2 cut(s)	1532, 2538
BstSCI	CCNGG	7 cut(s)	194, 922, 949, 961, 1019, 1598, 1796
BstSFI	CTRYAG	2 cut(s)	831, 2187
BstSLI	GKGCMC	2 cut(s)	1514, 1599
BstUI	CGCG	2 cut(s)	2981, 3006
BstV1I	GCAGC	8 cut(s)	280, 360, 577, 677, 836, 933, 3026, 3091
BstV2I	GAAGAC	3 cut(s)	354, 2562, 2696
BstX2I	RGATCY	5 cut(s)	319, 1216, 1931, 2124, 2205
BstXI	CCANNNNNNTGG	2 cut(s)	1054, 2074
BstYI	RGATCY	5 cut(s)	319, 1216, 1931, 2124, 2205
BstZ17I	GTATAC	2 cut(s)	1473, 2878
Bsu15I	ATCGAT	2 cut(s)	1347, 2619
BsuI	GTATCC	4 cut(s)	82, 113, 1017, 1922
BsuRI	GGCC	7 cut(s)	452, 479, 966, 1433, 1597, 2287, 2667
BsuTUI	ATCGAT	2 cut(s)	1347, 2619
BtgI	CCRYGG	1 cut(s)	1166
BtsCI	GGATG	4 cut(s)	217, 1063, 1453, 2361
BtsI	GCAGTG	1 cut(s)	3141
BtsIMutI	CAGTG	5 cut(s)	29, 814, 2136, 2801, 3141
BveI	ACCTGC	3 cut(s)	326, 2125, 2764
Cac8I	GCNNGC	1 cut(s)	2983
CaiI	CAGNNNCTG	1 cut(s)	830
CciI	TCATGA	1 cut(s)	1486
CfoI	GCGC	3 cut(s)	274, 2007, 3006
Cfr13I	GGNCC	4 cut(s)	451, 738, 1595, 1596
ClaI	ATCGAT	2 cut(s)	1347, 2619
CseI	GACGC	2 cut(s)	16, 547
Csp6I	GTAC	7 cut(s)	355, 579, 698, 764, 1241, 2304, 2855
CspCI	CAANNNNNGTGG	4 cut(s)	762, 797, 2325, 2360
CviQI	GTAC	7 cut(s)	355, 579, 698, 764, 1241, 2304, 2855
EaeI	YGGCCR	1 cut(s)	2285
Eam1104I	CTCTTC	2 cut(s)	1074, 2100
EarI	CTCTTC	2 cut(s)	1074, 2100
EciI	GGCGGA	1 cut(s)	3054
Ecl136II	GAGCTC	2 cut(s)	2110, 2736
Eco130I	CCWWGG	2 cut(s)	1166, 1750
Eco147I	AGGCCT	1 cut(s)	2667
Eco24I	GRGCYC	5 cut(s)	1040, 1253, 1599, 2112, 2738
Eco31I	GGTCTC	2 cut(s)	1997, 2172
Eco32I	GATATC	2 cut(s)	307, 2469
Eco47I	GGWCC	1 cut(s)	738
Eco47III	AGCGCT	1 cut(s)	273
Eco53kI	GAGCTC	2 cut(s)	2110, 2736
Eco88I	CYCGRG	2 cut(s)	158, 2455
EcoICRI	GAGCTC	2 cut(s)	2110, 2736
EcoNI	CCTNNNNNAGG	2 cut(s)	71, 314
EcoO109I	RGGNCCY	2 cut(s)	738, 1595
EcoRII	CCWGG	5 cut(s)	922, 949, 961, 1598, 1796
EcoRV	GATATC	2 cut(s)	307, 2469
EcoT14I	CCWWGG	2 cut(s)	1166, 1750
EcoT38I	GRGCYC	5 cut(s)	1040, 1253, 1599, 2112, 2738
ErhI	CCWWGG	2 cut(s)	1166, 1750
FalI	AAGNNNNNCTT	2 cut(s)	224, 256
FaqI	GGGAC	2 cut(s)	1693, 3153
FbaI	TGATCA	2 cut(s)	54, 2370
FblI	GTMKAC	5 cut(s)	634, 1472, 1894, 2877, 2976
FokI	GGATG	4 cut(s)	224, 1050, 1460, 2348
FriOI	GRGCYC	5 cut(s)	1040, 1253, 1599, 2112, 2738
FspBI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 2037, 3095
FspI	TGCGCA	1 cut(s)	2006
GlaI	GCGC	3 cut(s)	273, 2006, 3005
GsaI	CCCAGC	1 cut(s)	1623
GsuI	CTGGAG	3 cut(s)	2727, 3204, 3251
HaeII	RGCGCY	1 cut(s)	275
HaeIII	GGCC	7 cut(s)	452, 479, 966, 1433, 1597, 2287, 2667
HapII	CCGG	3 cut(s)	195, 599, 1021
HgaI	GACGC	2 cut(s)	16, 547
HhaI	GCGC	3 cut(s)	274, 2007, 3006
Hin1I	GRCGYC	2 cut(s)	8, 794
Hin6I	GCGC	3 cut(s)	272, 2005, 3004
HinP1I	GCGC	3 cut(s)	272, 2005, 3004
HincII	GTYRAC	2 cut(s)	786, 2100
HindII	GTYRAC	2 cut(s)	786, 2100
HindIII	AAGCTT	5 cut(s)	101, 467, 553, 1156, 2352
HpaI	GTTAAC	1 cut(s)	2100
HpaII	CCGG	3 cut(s)	195, 599, 1021
HphI	GGTGA	3 cut(s)	1331, 1433, 1832
Hpy99I	CGWCG	1 cut(s)	10
HpyCH4III	ACNGT	9 cut(s)	507, 884, 918, 2191, 2277, 2300, 2765, 2805, 3001
HpyCH4IV	ACGT	2 cut(s)	794, 969
HpySE526I	ACGT	2 cut(s)	794, 969
Hsp92I	GRCGYC	2 cut(s)	8, 794
HspAI	GCGC	3 cut(s)	272, 2005, 3004
Kpn2I	TCCGGA	1 cut(s)	598
Ksp22I	TGATCA	2 cut(s)	54, 2370
KspAI	GTTAAC	1 cut(s)	2100
LguI	GCTCTTC	1 cut(s)	2100
LmnI	GCTCC	5 cut(s)	692, 1248, 1498, 2708, 2733
Lsp1109I	GCAGC	8 cut(s)	280, 360, 577, 677, 836, 933, 3026, 3091
LweI	GCATC	8 cut(s)	1284, 1339, 1870, 1992, 2370, 2392, 2704, 3026
MaeI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 2037, 3095
MaeII	ACGT	2 cut(s)	794, 969
MaeIII	GTNAC	5 cut(s)	1820, 2329, 2530, 2645, 2659
MfeI	CAATTG	1 cut(s)	993
MflI	RGATCY	5 cut(s)	319, 1216, 1931, 2124, 2205
MhlI	GDGCHC	6 cut(s)	1040, 1253, 1514, 1599, 2112, 2738
MlsI	TGGCCA	1 cut(s)	2287
MluNI	TGGCCA	1 cut(s)	2287
MlyI	GAGTC	3 cut(s)	2667, 2857, 3188
MmeI	TCCRAC	4 cut(s)	666, 769, 1072, 2557
Mox20I	TGGCCA	1 cut(s)	2287
MroI	TCCGGA	1 cut(s)	598
MroXI	GAANNNNTTC	4 cut(s)	140, 236, 2765, 2843
MscI	TGGCCA	1 cut(s)	2287
MslI	CAYNNNNRTG	4 cut(s)	206, 1052, 1637, 2366
Msp20I	TGGCCA	1 cut(s)	2287
MspA1I	CMGCKG	1 cut(s)	373
MspCI	CTTAAG	1 cut(s)	461
MspI	CCGG	3 cut(s)	195, 599, 1021
MspR9I	CCNGG	7 cut(s)	196, 924, 951, 963, 1021, 1600, 1798
MunI	CAATTG	1 cut(s)	993
Mva1269I	GAATGC	3 cut(s)	568, 983, 2903
MvaI	CCWGG	5 cut(s)	924, 951, 963, 1600, 1798
MvnI	CGCG	2 cut(s)	2981, 3006
NciI	CCSGG	2 cut(s)	196, 1021
NcoI	CCATGG	1 cut(s)	1166
NmeAIII	GCCGAG	1 cut(s)	2137
NmuCI	GTSAC	3 cut(s)	1820, 2645, 2659
NsbI	TGCGCA	1 cut(s)	2006
NspI	RCATGY	2 cut(s)	1532, 2538
NspV	TTCGAA	1 cut(s)	243
OliI	CACNNNNGTG	1 cut(s)	206
PaeR7I	CTCGAG	1 cut(s)	158
PagI	TCATGA	1 cut(s)	1486
PasI	CCCWGGG	2 cut(s)	923, 1599
PceI	AGGCCT	1 cut(s)	2667
PciI	ACATGT	2 cut(s)	1528, 2534
PciSI	GCTCTTC	1 cut(s)	2100
PcsI	WCGNNNNNNNCGW	3 cut(s)	14, 20, 2856
PctI	GAATGC	3 cut(s)	568, 983, 2903
PdmI	GAANNNNTTC	4 cut(s)	140, 236, 2765, 2843
PleI	GAGTC	3 cut(s)	2666, 2857, 3187
PpsI	GAGTC	3 cut(s)	2666, 2857, 3187
PpuMI	RGGWCCY	1 cut(s)	738
PscI	ACATGT	2 cut(s)	1528, 2534
Psp124BI	GAGCTC	2 cut(s)	2112, 2738
Psp5II	RGGWCCY	1 cut(s)	738
Psp6I	CCWGG	5 cut(s)	922, 949, 961, 1598, 1796
PspFI	CCCAGC	1 cut(s)	1619
PspGI	CCWGG	5 cut(s)	922, 949, 961, 1598, 1796
PspOMI	GGGCCC	1 cut(s)	1595
PspPI	GGNCC	4 cut(s)	451, 738, 1595, 1596
PspPPI	RGGWCCY	1 cut(s)	738
PspXI	VCTCGAGB	1 cut(s)	158
PstI	CTGCAG	1 cut(s)	835
PstNI	CAGNNNCTG	1 cut(s)	830
PsuI	RGATCY	5 cut(s)	319, 1216, 1931, 2124, 2205
PvuII	CAGCTG	1 cut(s)	373
RsaI	GTAC	7 cut(s)	356, 580, 699, 765, 1242, 2305, 2856
RsaNI	GTAC	7 cut(s)	355, 579, 698, 764, 1241, 2304, 2855
RseI	CAYNNNNRTG	4 cut(s)	206, 1052, 1637, 2366
SacI	GAGCTC	2 cut(s)	2112, 2738
SapI	GCTCTTC	1 cut(s)	2100
Sau96I	GGNCC	4 cut(s)	451, 738, 1595, 1596
SchI	GAGTC	3 cut(s)	2667, 2857, 3188
ScrFI	CCNGG	7 cut(s)	196, 924, 951, 963, 1021, 1600, 1798
SduI	GDGCHC	6 cut(s)	1040, 1253, 1514, 1599, 2112, 2738
SfaNI	GCATC	8 cut(s)	1284, 1339, 1870, 1992, 2370, 2392, 2704, 3026
SfcI	CTRYAG	2 cut(s)	831, 2187
Sfr274I	CTCGAG	1 cut(s)	158
SfuI	TTCGAA	1 cut(s)	243
SinI	GGWCC	1 cut(s)	738
SlaI	CTCGAG	1 cut(s)	158
SmiMI	CAYNNNNRTG	4 cut(s)	206, 1052, 1637, 2366
SmlI	CTYRAG	6 cut(s)	158, 461, 2209, 2639, 2887, 3176
SmoI	CTYRAG	6 cut(s)	158, 461, 2209, 2639, 2887, 3176
SpeI	ACTAGT	1 cut(s)	1097
SseBI	AGGCCT	1 cut(s)	2667
SsiI	CCGC	6 cut(s)	220, 297, 1419, 1915, 3065, 3203
SspMI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 2037, 3095
SstI	GAGCTC	2 cut(s)	2112, 2738
StuI	AGGCCT	1 cut(s)	2667
StyD4I	CCNGG	7 cut(s)	194, 922, 949, 961, 1019, 1598, 1796
StyI	CCWWGG	2 cut(s)	1166, 1750
TaaI	ACNGT	9 cut(s)	507, 884, 918, 2191, 2277, 2300, 2765, 2805, 3001
TaiI	ACGT	2 cut(s)	797, 972
TaqII	GACCGA	1 cut(s)	2154
TatI	WGTACW	5 cut(s)	354, 578, 697, 1240, 2854
TauI	GCSGC	2 cut(s)	299, 3205
TscAI	CASTG	5 cut(s)	36, 814, 2143, 2808, 3141
TseFI	GTSAC	3 cut(s)	1820, 2645, 2659
TseI	GCWGC	8 cut(s)	293, 373, 565, 665, 824, 946, 3014, 3104
Tsp45I	GTSAC	3 cut(s)	1820, 2645, 2659
TspGWI	ACGGA	3 cut(s)	482, 550, 2603
TspRI	CASTG	5 cut(s)	36, 814, 2143, 2808, 3141
Vha464I	CTTAAG	1 cut(s)	461
VpaK11BI	GGWCC	1 cut(s)	738
XagI	CCTNNNNNAGG	2 cut(s)	71, 314
XapI	RAATTY	3 cut(s)	246, 2339, 2506
XbaI	TCTAGA	1 cut(s)	2036
XceI	RCATGY	2 cut(s)	1532, 2538
XhoI	CTCGAG	1 cut(s)	158
XmiI	GTMKAC	5 cut(s)	634, 1472, 1894, 2877, 2976
XmnI	GAANNNNTTC	4 cut(s)	140, 236, 2765, 2843
XspI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 2037, 3095
ZraI	GACGTC	1 cut(s)	795

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.