Rroxscaffold_7G00158210

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006217
Physical Location & Seq
Forward (+)
1724614 .. 1730306
5693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00158210.1

Sequence Viewer

Length: 2802 bp
ATGTCGAAGATGAAGCACCTACTGAGAAAGCTTCACATCGGCGGGGACCAGAGGCTGGCGGAGACGACGGCCGCGGTGGCCCCGCCGGTGGTGGCGAGTCCGGGGACGGCGAATCTCAACCCGACGGCGTCGTCACCGGCGTCGTCGTCGTCGGGGTCGGCGACAATGGGAAGAATCGCGGCGGTGGAGTCGGTGAGTGATCGGAAGTCTGGCGACGGCGGAGGTAGCGGGAGTGGCGGAGGAGGCGTGGATTTCAATTTCTTGGAGGAGGAGTTTCAGGTACAGTTGGCCCTAGCGATTAGCGCTTCCGATCCCGATTCGCGCGACGACCCCGAGACGGCTCAGATCGACGCCGCGAAACGGATTAGCCTCGGCTGCGCGGCGACGTCGCTCGCCGACGCACAAGCCCCTTTTCAGATGCTTTCGCTTCGCTACTGGAGCCATAATGTCGTAGATTATAATGAAAAAGTGGTGGATGGATTTTACGATGTGTATGGAATTACCTCGAATTCATTTAGACAAGGGAAGATGCCATTGTTGGTGGAATTTCGAGCGGTATCGGTTTCAGATAATGTCGACTACGATGTCATATTAGTTAACCGGTATGTTGATACTGAACTGCAACAACTCGAGAAAAGAGCATATGCTGCATCTCTAGAGTCGGGGATTTCTCAACATGGTCTACTTTTAAGTGGCTTGATTCAGAAGATTGCTGATATTGTTGTTGATAGAATGGGTGGTCCAGTTGGAGATGCGGATGAGATTTTAAGAAGGTGGAAAATAAGGAGGCATGAATTGCGGAGTTCAATGAACACTATCATTCTTCCCCTCGGAAAAATTGATGTTGGACTTTCACGCCACCGGGCCCTGCTCTTTAAGGTATTAGCTGATAAGATAAATCTTCCATGTATGCTGGTCAAAGGTAGCTACTACACTGGTACTGATGATGGAGCTGTAAACTTGATTAAAATTGATAGTGGAATTGGAAGCGAATATATTATTGATCTGATGGGTGCTCCTGGAACACTAATTCCTGCTGAGGTACCTACTAGTCAGCTCCCAAATTCTTTTTTTGCCATTAGGAGCTTTCAAGATCCGACAGAAATTCCTACAGAGATGCCCAAAGATCTGTGTTTACTACAAGCTGAGGGAACTGGAATGTCGGCAGCTCCACCTGGTCTTGAAAGAGCTTCTACATTTGGCAGCTCAAGGTCAGAAGAAGCATCATATGCAGGTGTTCAAACAAAAGATGACCAAAGAAGTGTTGTGGATGAAAATCAAATTGAGATTCTTAAAAGTGAGCTTGAGACTCCTGTTAAAAGTAAATCATGTGGAAGTTCATCAGGCCCATCTGGGAAAGCTACATCTGCACAAAAAAGAAAAGTGAAAAATGTATCAAGGTATGTCATCAGTGCCGCAAAGAACCCAGAATTTGCACAGAAACTACATGCTGTCTTGTTGGAGAGCGGTGCATCACCTCCTCCCGACTTATTTTCAGATATGAATCCTCAATACCTGAATGAAGGTAAATTGCTTGGACAAATCCATGCAAATGGGGAACTTGTAGATGAGGGGGTTCATGATTATCTGGTTAAACTATTGTCAAGCAGTGATCAGTCCTCTGCCGTGGATGTAGCTGAGCAACGGAATGAGTGGAGGACGAATTCTTTTCTCTCTGATAATGTTGATGAGGGCTTTGTAATGGTTTCTGGCAGAACTAGTGAAACAACCCAGATTGGTGCTATAAATTTAGATCCTGCTTTTGGTAATCCCCCAAGAATGAATGCAGAAGCTTTTCATGAGGAGAAAATTCATGATATATCTATGGTCTCTGGAACAACTTCTGCTAACACTCAATTGGGCAAAGAATCTATTGCTCAATCAACGCAAACAGCCAAGAGCAGCCTGAATGCTGCTTGGGATAGTCACGTTGATAGGTACCCAGCGTTGGGAGAAGTTGCGGAGTGGGAAATTCTATGGGAGGATCTTCAGATTGGAGAGCGCATTGGCATTGGTTCATATGGTGAGGTATATCATGCAGATTGGAATGGCACTGAAGTAGCTGTGAAGAAATTTTTAGATCAAGATTTTTCGGGTGATGCATTGGTTCAGTTTAGATGTGAAGTAGAGATCATGTTAAGACTTCGACATCCAAATGTTGTGCTTTTCATGGGCGCTGTTACTCGCCCTCCCCATTTCTCCATCCTGACAGAGTTTCTTCCCAGGGGTAGCTTATACCGATTACTGCATCGTCCAAATTCTCAACTTGATGAAAAGAGGCGAATGCGAATGGCTCTTGATGTGGCGAAGGGAATGAATTACCTCCACACCAGCAATCCTACAGTTGTGCATCGAGACCTTAAGTCTCCAAATCTCCTTGTTGATAAGAATTGGAATGTGAAGGTTTGTGACTTTGGGTTGTCACGTACGAAGCACCACACTTATCTGTCCTCAAAGTCCACTGCTGGAACGCCGGAATGGATGGCACCGGAAGTTTTAAGGAATGAATTAGCTAACGAGAAGTGTGATGTGTACAGTTTTGGTGTGATATTATGGGAATTGACTACTTGCTGTATCCCATGGAAAGGATTGAACCCAATGCAGGTTGTTGGAGCTGTTGGATTCCAAAATAGGCGTCTTGAAATTCCGGATGACGTTGACCCAGTGGTTGCCGAGATAATACGTGATTGTTGGCAAACAGAGCCAAATCTACGGCCTTCTTTCTCCCAGCTCATGGTCCGACTCAAGCGTCTTCAACGGCTTGTTGGAAGAACGAACTCTGCAAATCAAATGACTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

933

Amino Acids

102.26

Weight (kDa)

5.58

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 139 - 344 6.3e-71 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 663 - 914 4.6e-66 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 664 - 912 2e-51 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 459
AarI CACCTGC 1 cut(s) 1223
AasI GACNNNNNNGTC 3 cut(s) 130, 584, 2748
AatII GACGTC 1 cut(s) 389
Acc36I ACCTGC 2 cut(s) 1223, 2593
Acc65I GGTACC 2 cut(s) 1042, 1938
AccB1I GGYRCC 3 cut(s) 1042, 1938, 2485
AccB7I CCANNNNNTGG 2 cut(s) 55, 2734
AccBSI CCGCTC 2 cut(s) 554, 1467
AccI GTMKAC 2 cut(s) 576, 682
AccII CGCG 6 cut(s) 74, 179, 322, 324, 356, 380
AccIII TCCGGA 1 cut(s) 2647
AclWI GGATC 4 cut(s) 305, 1088, 1748, 1992
AcoI YGGCCR 1 cut(s) 69
AcuI CTGAAG 2 cut(s) 1973, 2076
AcyI GRCGYC 5 cut(s) 128, 140, 351, 386, 2635
AfaI GTAC 6 cut(s) 282, 940, 1044, 1940, 2428, 2534
AfeI AGCGCT 1 cut(s) 304
AflII CTTAAG 1 cut(s) 2360
AgeI ACCGGT 1 cut(s) 600
AgsI TTSAA 8 cut(s) 256, 807, 1091, 1184, 1241, 2593, 2642, 2756
AhdI GACNNNNNGTC 1 cut(s) 2362
AhlI ACTAGT 2 cut(s) 1049, 1718
AjnI CCWGG 3 cut(s) 1018, 1174, 2222
AloI GAACNNNNNNTCC 2 cut(s) 1015, 1047
Alw21I GWGCWC 1 cut(s) 1018
Alw26I GTCTC 6 cut(s) 56, 329, 1301, 1834, 2349, 2370
AlwI GGATC 4 cut(s) 305, 1088, 1748, 1992
AlwNI CAGNNNCTG 1 cut(s) 55
Ama87I CYCGRG 2 cut(s) 332, 629
Aor13HI TCCGGA 1 cut(s) 2647
Aor51HI AGCGCT 1 cut(s) 304
AoxI GGCC 6 cut(s) 69, 78, 288, 864, 1345, 2714
ApaI GGGCCC 1 cut(s) 868
ApeKI GCWGC 6 cut(s) 375, 647, 1166, 1203, 1902, 1913
ArsI GACNNNNNNTTYG 2 cut(s) 2620, 2652
AsiGI ACCGGT 1 cut(s) 600
Asp700I GAANNNNTTC 2 cut(s) 1794, 1840
Asp718I GGTACC 2 cut(s) 1042, 1938
AspLEI GCGC 5 cut(s) 305, 324, 380, 2004, 2177
AspS9I GGNCC 8 cut(s) 46, 79, 289, 740, 864, 865, 1346, 2737
AsuC2I CCSGG 2 cut(s) 102, 863
AsuHPI GGTGA 5 cut(s) 126, 205, 1467, 2036, 2108
AvaI CYCGRG 2 cut(s) 332, 629
AvaII GGWCC 3 cut(s) 46, 740, 2737
BaeGI GKGCMC 1 cut(s) 868
BanI GGYRCC 3 cut(s) 1042, 1938, 2485
BanII GRGCYC 1 cut(s) 868
BbsI GAAGAC 1 cut(s) 2744
Bbv12I GWGCWC 1 cut(s) 1018
BbvCI CCTCAGC 2 cut(s) 1038, 1146
BbvI GCAGC 6 cut(s) 362, 634, 1178, 1215, 1900, 1914
BccI CCATC 6 cut(s) 470, 941, 1003, 1357, 2210, 2476
BceAI ACGGC 8 cut(s) 84, 123, 141, 232, 354, 1610, 2729, 2774
BciT130I CCWGG 3 cut(s) 1020, 1176, 2224
BciVI GTATCC 1 cut(s) 2585
BclI TGATCA 1 cut(s) 1612
BcnI CCSGG 2 cut(s) 102, 863
BcoDI GTCTC 6 cut(s) 56, 329, 1301, 1834, 2349, 2370
BcuI ACTAGT 2 cut(s) 1049, 1718
BfaI CTAG 4 cut(s) 293, 656, 1050, 1719
BfmI CTRYAG 2 cut(s) 1110, 2340
BfoI RGCGCY 2 cut(s) 306, 2178
BfrI CTTAAG 1 cut(s) 2360
BfuAI ACCTGC 2 cut(s) 1223, 2593
BfuI GTATCC 1 cut(s) 2585
BglI GCCNNNNNGGC 1 cut(s) 77
BglII AGATCT 1 cut(s) 1126
BlpI GCTNAGC 1 cut(s) 1638
Bme1390I CCNGG 5 cut(s) 102, 863, 1020, 1176, 2224
Bme18I GGWCC 3 cut(s) 46, 740, 2737
BmeRI GACNNNNNGTC 1 cut(s) 2362
BmeT110I CYCGRG 2 cut(s) 332, 629
BmgT120I GGNCC 8 cut(s) 46, 79, 289, 740, 864, 865, 1346, 2737
BmiI GGNNCC 7 cut(s) 47, 81, 440, 866, 1044, 1940, 2487
BmrFI CCNGG 5 cut(s) 102, 863, 1020, 1176, 2224
BmrI ACTGGG 1 cut(s) 2657
BmuI ACTGGG 1 cut(s) 2657
BpiI GAAGAC 1 cut(s) 2744
BpmI CTGGAG 1 cut(s) 457
Bpu10I CCTNAGC 2 cut(s) 1038, 1146
Bpu1102I GCTNAGC 1 cut(s) 1638
BpuEI CTTGAG 3 cut(s) 1192, 1325, 2729
BpuMI CCSGG 2 cut(s) 102, 863
BsaAI YACGTR 2 cut(s) 2426, 2684
BsaBI GATNNNNATC 2 cut(s) 1275, 1503
BsaHI GRCGYC 5 cut(s) 128, 140, 351, 386, 2635
BsaI GGTCTC 2 cut(s) 1834, 2349
BsaJI CCNNGG 8 cut(s) 72, 101, 370, 829, 1626, 2222, 2223, 2579
BsaWI WCCGGW 3 cut(s) 600, 2488, 2647
BsaXI ACNNNNNCTCC 6 cut(s) 231, 257, 261, 287, 2173, 2203
Bse118I RCCGGY 3 cut(s) 85, 136, 600
Bse1I ACTGG 5 cut(s) 440, 743, 940, 1159, 2663
Bse8I GATNNNNATC 2 cut(s) 1275, 1503
BseAI TCCGGA 1 cut(s) 2647
BseBI CCWGG 3 cut(s) 1020, 1176, 2224
BseDI CCNNGG 8 cut(s) 72, 101, 370, 829, 1626, 2222, 2223, 2579
BseGI GGATG 8 cut(s) 481, 763, 1276, 1636, 2149, 2202, 2487, 2656
BseJI GATNNNNATC 2 cut(s) 1275, 1503
BseMII CTCAG 5 cut(s) 14, 356, 1029, 1137, 1629
BseNI ACTGG 5 cut(s) 440, 743, 940, 1159, 2663
BseRI GAGGAG 5 cut(s) 255, 281, 284, 1470, 1817
BseSI GKGCMC 1 cut(s) 868
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 6 cut(s) 362, 634, 1178, 1215, 1900, 1914
BseYI CCCAGC 2 cut(s) 1942, 2727
BsgI GTGCAG 1 cut(s) 1353
Bsh1236I CGCG 6 cut(s) 74, 179, 322, 324, 356, 380
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 6 cut(s) 71, 80, 290, 866, 1347, 2716
BshNI GGYRCC 3 cut(s) 1042, 1938, 2485
BshTI ACCGGT 1 cut(s) 600
BsiEI CGRYCG 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 1018
BsiHKCI CYCGRG 2 cut(s) 332, 629
BsiSI CCGG 8 cut(s) 86, 101, 137, 601, 862, 2474, 2489, 2648
BsiWI CGTACG 1 cut(s) 2426
BslFI GGGAC 2 cut(s) 59, 118
BsmAI GTCTC 6 cut(s) 56, 329, 1301, 1834, 2349, 2370
BsmBI CGTCTC 2 cut(s) 56, 329
BsmFI GGGAC 2 cut(s) 59, 118
BsmI GAATGC 3 cut(s) 1789, 1915, 2289
BsnI GGCC 6 cut(s) 71, 80, 290, 866, 1347, 2716
Bso31I GGTCTC 2 cut(s) 1834, 2349
BsoBI CYCGRG 2 cut(s) 332, 629
Bsp120I GGGCCC 1 cut(s) 864
Bsp1286I GDGCHC 2 cut(s) 868, 1018
Bsp13I TCCGGA 1 cut(s) 2647
Bsp1407I TGTACA 1 cut(s) 2532
Bsp1720I GCTNAGC 1 cut(s) 1638
Bsp19I CCATGG 1 cut(s) 2579
BspANI GGCC 6 cut(s) 71, 80, 290, 866, 1347, 2716
BspCNI CTCAG 5 cut(s) 15, 355, 1030, 1138, 1630
BspEI TCCGGA 1 cut(s) 2647
BspFNI CGCG 6 cut(s) 74, 179, 322, 324, 356, 380
BspHI TCATGA 3 cut(s) 1579, 1798, 1813
BspLI GGNNCC 7 cut(s) 47, 81, 440, 866, 1044, 1940, 2487
BspMI ACCTGC 2 cut(s) 1223, 2593
BspPI GGATC 4 cut(s) 305, 1088, 1748, 1992
BspT107I GGYRCC 3 cut(s) 1042, 1938, 2485
BspTI CTTAAG 1 cut(s) 2360
BspTNI GGTCTC 2 cut(s) 1834, 2349
BsrBI CCGCTC 2 cut(s) 554, 1467
BsrFI RCCGGY 3 cut(s) 85, 136, 600
BsrGI TGTACA 1 cut(s) 2532
BsrI ACTGG 5 cut(s) 440, 743, 940, 1159, 2663
BssAI RCCGGY 3 cut(s) 85, 136, 600
BssECI CCNNGG 8 cut(s) 72, 101, 370, 829, 1626, 2222, 2223, 2579
BssNI GRCGYC 5 cut(s) 128, 140, 351, 386, 2635
BssT1I CCWWGG 1 cut(s) 2579
Bst2UI CCWGG 3 cut(s) 1020, 1176, 2224
Bst4CI ACNGT 3 cut(s) 285, 2344, 2537
BstACI GRCGYC 5 cut(s) 128, 140, 351, 386, 2635
BstAFI CTTAAG 1 cut(s) 2360
BstAPI GCANNNNNTGC 3 cut(s) 647, 796, 1229
BstAUI TGTACA 1 cut(s) 2532
BstBAI YACGTR 2 cut(s) 2426, 2684
BstC8I GCNNGC 2 cut(s) 57, 393
BstDEI CTNAG 5 cut(s) 23, 342, 1038, 1146, 1638
BstDSI CCRYGG 3 cut(s) 72, 1626, 2579
BstF5I GGATG 8 cut(s) 481, 763, 1276, 1636, 2149, 2202, 2487, 2656
BstFNI CGCG 6 cut(s) 74, 179, 322, 324, 356, 380
BstH2I RGCGCY 2 cut(s) 306, 2178
BstHHI GCGC 5 cut(s) 305, 324, 380, 2004, 2177
BstMAI GTCTC 6 cut(s) 56, 329, 1301, 1834, 2349, 2370
BstMCI CGRYCG 1 cut(s) 72
BstNI CCWGG 3 cut(s) 1020, 1176, 2224
BstNSI RCATGY 1 cut(s) 1451
BstSCI CCNGG 5 cut(s) 100, 861, 1018, 1174, 2222
BstSFI CTRYAG 2 cut(s) 1110, 2340
BstSLI GKGCMC 1 cut(s) 868
BstUI CGCG 6 cut(s) 74, 179, 322, 324, 356, 380
BstV1I GCAGC 6 cut(s) 362, 634, 1178, 1215, 1900, 1914
BstV2I GAAGAC 1 cut(s) 2744
BstX2I RGATCY 4 cut(s) 1093, 1126, 1753, 1984
BstXI CCANNNNNNTGG 1 cut(s) 1553
BstYI RGATCY 4 cut(s) 1093, 1126, 1753, 1984
BstZI CGGCCG 1 cut(s) 69
BsuI GTATCC 1 cut(s) 2585
BsuRI GGCC 6 cut(s) 71, 80, 290, 866, 1347, 2716
BtgI CCRYGG 3 cut(s) 72, 1626, 2579
BtsCI GGATG 8 cut(s) 481, 763, 1276, 1636, 2149, 2202, 2487, 2656
BtsI GCAGTG 2 cut(s) 1615, 2460
BtsIMutI CAGTG 6 cut(s) 933, 1417, 1615, 2052, 2460, 2670
BveI ACCTGC 2 cut(s) 1223, 2593
Cac8I GCNNGC 2 cut(s) 57, 393
CaiI CAGNNNCTG 1 cut(s) 55
CciI TCATGA 3 cut(s) 1579, 1798, 1813
CfoI GCGC 5 cut(s) 305, 324, 380, 2004, 2177
Cfr10I RCCGGY 3 cut(s) 85, 136, 600
Cfr13I GGNCC 8 cut(s) 46, 79, 289, 740, 864, 865, 1346, 2737
Cfr42I CCGCGG 1 cut(s) 75
CseI GACGC 6 cut(s) 117, 129, 359, 407, 2624, 2738
CsiI ACCWGGT 1 cut(s) 1174
Csp6I GTAC 6 cut(s) 281, 939, 1043, 1939, 2427, 2533
CspAI ACCGGT 1 cut(s) 600
CviQI GTAC 6 cut(s) 281, 939, 1043, 1939, 2427, 2533
DdeI CTNAG 5 cut(s) 23, 342, 1038, 1146, 1638
DrdI GACNNNNNNGTC 3 cut(s) 130, 584, 2748
DriI GACNNNNNGTC 1 cut(s) 2362
DseDI GACNNNNNNGTC 3 cut(s) 130, 584, 2748
EaeI YGGCCR 1 cut(s) 69
EagI CGGCCG 1 cut(s) 69
Eam1105I GACNNNNNGTC 1 cut(s) 2362
EciI GGCGGA 3 cut(s) 74, 234, 252
EclXI CGGCCG 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 2579
Eco24I GRGCYC 1 cut(s) 868
Eco31I GGTCTC 2 cut(s) 1834, 2349
Eco47I GGWCC 3 cut(s) 46, 740, 2737
Eco47III AGCGCT 1 cut(s) 304
Eco52I CGGCCG 1 cut(s) 69
Eco57I CTGAAG 2 cut(s) 1973, 2076
Eco88I CYCGRG 2 cut(s) 332, 629
EcoO109I RGGNCCY 1 cut(s) 865
EcoRI GAATTC 2 cut(s) 508, 1663
EcoRII CCWGG 3 cut(s) 1018, 1174, 2222
EcoT14I CCWWGG 1 cut(s) 2579
EcoT22I ATGCAT 1 cut(s) 2104
EcoT38I GRGCYC 1 cut(s) 868
ErhI CCWWGG 1 cut(s) 2579
Esp3I CGTCTC 2 cut(s) 56, 329
FaqI GGGAC 2 cut(s) 59, 118
FauI CCCGC 3 cut(s) 35, 90, 221
FauNDI CATATG 3 cut(s) 643, 1228, 2020
FbaI TGATCA 1 cut(s) 1612
FblI GTMKAC 2 cut(s) 576, 682
FokI GGATG 8 cut(s) 488, 770, 1283, 1643, 2136, 2189, 2494, 2663
FriOI GRGCYC 1 cut(s) 868
FspBI CTAG 4 cut(s) 293, 656, 1050, 1719
GlaI GCGC 5 cut(s) 304, 323, 379, 2003, 2176
GsaI CCCAGC 2 cut(s) 1946, 2731
GsuI CTGGAG 1 cut(s) 457
HaeII RGCGCY 2 cut(s) 306, 2178
HaeIII GGCC 6 cut(s) 71, 80, 290, 866, 1347, 2716
HapII CCGG 8 cut(s) 86, 101, 137, 601, 862, 2474, 2489, 2648
HgaI GACGC 6 cut(s) 117, 129, 359, 407, 2624, 2738
HhaI GCGC 5 cut(s) 305, 324, 380, 2004, 2177
Hin1I GRCGYC 5 cut(s) 128, 140, 351, 386, 2635
Hin6I GCGC 5 cut(s) 303, 322, 378, 2002, 2175
HinP1I GCGC 5 cut(s) 303, 322, 378, 2002, 2175
HincII GTYRAC 3 cut(s) 577, 598, 2659
HindII GTYRAC 3 cut(s) 577, 598, 2659
HindIII AAGCTT 2 cut(s) 29, 1791
HpaI GTTAAC 1 cut(s) 598
HpaII CCGG 8 cut(s) 86, 101, 137, 601, 862, 2474, 2489, 2648
HphI GGTGA 5 cut(s) 126, 205, 1467, 2036, 2108
Hpy166II GTNNAC 8 cut(s) 577, 598, 683, 958, 1136, 2460, 2533, 2659
Hpy8I GTNNAC 8 cut(s) 577, 598, 683, 958, 1136, 2460, 2533, 2659
HpyAV CCTTC 5 cut(s) 765, 1517, 2302, 2395, 2727
HpyCH4III ACNGT 3 cut(s) 285, 2344, 2537
HpyCH4IV ACGT 5 cut(s) 386, 1929, 2425, 2655, 2683
HpyF3I CTNAG 5 cut(s) 23, 342, 1038, 1146, 1638
HpySE526I ACGT 5 cut(s) 386, 1929, 2425, 2655, 2683
Hsp92I GRCGYC 5 cut(s) 128, 140, 351, 386, 2635
HspAI GCGC 5 cut(s) 303, 322, 378, 2002, 2175
Kpn2I TCCGGA 1 cut(s) 2647
KpnI GGTACC 2 cut(s) 1046, 1942
Ksp22I TGATCA 1 cut(s) 1612
KspAI GTTAAC 1 cut(s) 598
KspI CCGCGG 1 cut(s) 75
LmnI GCTCC 7 cut(s) 438, 950, 1021, 1062, 1083, 1174, 2612
Lsp1109I GCAGC 6 cut(s) 362, 634, 1178, 1215, 1900, 1914
MabI ACCWGGT 1 cut(s) 1174
MaeI CTAG 4 cut(s) 293, 656, 1050, 1719
MaeII ACGT 5 cut(s) 386, 1929, 2425, 2655, 2683
MaeIII GTNAC 5 cut(s) 132, 1925, 2179, 2408, 2421
MbiI CCGCTC 2 cut(s) 554, 1467
MfeI CAATTG 1 cut(s) 1856
MflI RGATCY 4 cut(s) 1093, 1126, 1753, 1984
MhlI GDGCHC 2 cut(s) 868, 1018
MlyI GAGTC 6 cut(s) 106, 197, 668, 1303, 2736, 2788
MmeI TCCRAC 8 cut(s) 727, 826, 1121, 1440, 2590, 2599, 2746, 2764
Mph1103I ATGCAT 1 cut(s) 2104
MroI TCCGGA 1 cut(s) 2647
MroXI GAANNNNTTC 2 cut(s) 1794, 1840
MslI CAYNNNNRTG 2 cut(s) 1551, 2154
MspA1I CMGCKG 1 cut(s) 74
MspCI CTTAAG 1 cut(s) 2360
MspI CCGG 8 cut(s) 86, 101, 137, 601, 862, 2474, 2489, 2648
MspR9I CCNGG 5 cut(s) 102, 863, 1020, 1176, 2224
MunI CAATTG 1 cut(s) 1856
Mva1269I GAATGC 3 cut(s) 1789, 1915, 2289
MvaI CCWGG 3 cut(s) 1020, 1176, 2224
MvnI CGCG 6 cut(s) 74, 179, 322, 324, 356, 380
NciI CCSGG 2 cut(s) 102, 863
NcoI CCATGG 1 cut(s) 2579
NdeI CATATG 3 cut(s) 643, 1228, 2020
NlaIV GGNNCC 7 cut(s) 47, 81, 440, 866, 1044, 1940, 2487
NmeAIII GCCGAG 2 cut(s) 351, 2698
NmuCI GTSAC 4 cut(s) 132, 1925, 2408, 2421
NsiI ATGCAT 1 cut(s) 2104
NspI RCATGY 1 cut(s) 1451
PaeR7I CTCGAG 1 cut(s) 629
PagI TCATGA 3 cut(s) 1579, 1798, 1813
PaqCI CACCTGC 1 cut(s) 1223
PasI CCCWGGG 1 cut(s) 2223
PcsI WCGNNNNNNNCGW 2 cut(s) 137, 158
PctI GAATGC 3 cut(s) 1789, 1915, 2289
PdmI GAANNNNTTC 2 cut(s) 1794, 1840
PfeI GAWTC 8 cut(s) 112, 174, 317, 700, 1288, 1504, 1868, 2622
Pfl23II CGTACG 1 cut(s) 2426
PflFI GACNNNGTC 1 cut(s) 127
PflMI CCANNNNNTGG 2 cut(s) 55, 2734
PfoI TCCNGGA 1 cut(s) 1018
PinAI ACCGGT 1 cut(s) 600
PleI GAGTC 6 cut(s) 105, 196, 667, 1303, 2736, 2788
PpsI GAGTC 6 cut(s) 105, 196, 667, 1303, 2736, 2788
Ppu21I YACGTR 2 cut(s) 2426, 2684
PsiI TTATAA 1 cut(s) 459
Psp6I CCWGG 3 cut(s) 1018, 1174, 2222
PspFI CCCAGC 2 cut(s) 1942, 2727
PspGI CCWGG 3 cut(s) 1018, 1174, 2222
PspLI CGTACG 1 cut(s) 2426
PspN4I GGNNCC 7 cut(s) 47, 81, 440, 866, 1044, 1940, 2487
PspOMI GGGCCC 1 cut(s) 864
PspPI GGNCC 8 cut(s) 46, 79, 289, 740, 864, 865, 1346, 2737
PstNI CAGNNNCTG 1 cut(s) 55
PsuI RGATCY 4 cut(s) 1093, 1126, 1753, 1984
PsyI GACNNNGTC 1 cut(s) 127
RsaI GTAC 6 cut(s) 282, 940, 1044, 1940, 2428, 2534
RsaNI GTAC 6 cut(s) 281, 939, 1043, 1939, 2427, 2533
RseI CAYNNNNRTG 2 cut(s) 1551, 2154
SacII CCGCGG 1 cut(s) 75
SalI GTCGAC 1 cut(s) 575
Sau96I GGNCC 8 cut(s) 46, 79, 289, 740, 864, 865, 1346, 2737
SchI GAGTC 6 cut(s) 106, 197, 668, 1303, 2736, 2788
ScrFI CCNGG 5 cut(s) 102, 863, 1020, 1176, 2224
SduI GDGCHC 2 cut(s) 868, 1018
SexAI ACCWGGT 1 cut(s) 1174
SfcI CTRYAG 2 cut(s) 1110, 2340
SfiI GGCCNNNNNGGCC 1 cut(s) 77
Sfr274I CTCGAG 1 cut(s) 629
Sfr303I CCGCGG 1 cut(s) 75
SgrAI CRCCGGYG 2 cut(s) 85, 136
SgrBI CCGCGG 1 cut(s) 75
SinI GGWCC 3 cut(s) 46, 740, 2737
SlaI CTCGAG 1 cut(s) 629
SmiMI CAYNNNNRTG 2 cut(s) 1551, 2154
SmlI CTYRAG 5 cut(s) 629, 1207, 1304, 2360, 2744
SmoI CTYRAG 5 cut(s) 629, 1207, 1304, 2360, 2744
SpeI ACTAGT 2 cut(s) 1049, 1718
SspMI CTAG 4 cut(s) 293, 656, 1050, 1719
StyD4I CCNGG 5 cut(s) 100, 861, 1018, 1174, 2222
StyI CCWWGG 1 cut(s) 2579
TaaI ACNGT 3 cut(s) 285, 2344, 2537
TaiI ACGT 5 cut(s) 389, 1932, 2428, 2658, 2686
TaqI TCGA 9 cut(s) 5, 348, 506, 550, 576, 630, 2146, 2353, 2797
TatI WGTACW 1 cut(s) 2532
TauI GCSGC 5 cut(s) 74, 182, 356, 383, 1418
TfiI GAWTC 8 cut(s) 112, 174, 317, 700, 1288, 1504, 1868, 2622
TscAI CASTG 6 cut(s) 940, 1417, 1615, 2059, 2467, 2670
TseFI GTSAC 4 cut(s) 132, 1925, 2408, 2421
TseI GCWGC 6 cut(s) 375, 647, 1166, 1203, 1902, 1913
Tsp45I GTSAC 4 cut(s) 132, 1925, 2408, 2421
TspGWI ACGGA 2 cut(s) 376, 1660
TspRI CASTG 6 cut(s) 940, 1417, 1615, 2059, 2467, 2670
Tth111I GACNNNGTC 1 cut(s) 127
Van91I CCANNNNNTGG 2 cut(s) 55, 2734
Vha464I CTTAAG 1 cut(s) 2360
VpaK11BI GGWCC 3 cut(s) 46, 740, 2737
XbaI TCTAGA 1 cut(s) 655
XceI RCATGY 1 cut(s) 1451
XhoI CTCGAG 1 cut(s) 629
XmiI GTMKAC 2 cut(s) 576, 682
XmnI GAANNNNTTC 2 cut(s) 1794, 1840
XspI CTAG 4 cut(s) 293, 656, 1050, 1719
ZraI GACGTC 1 cut(s) 387
Zsp2I ATGCAT 1 cut(s) 2104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.