MD08G1061200.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006217
Physical Location & Seq
Reverse (-)
4836841 .. 4843188
6348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1061200.v1.1.491

Sequence Viewer

Length: 2772 bp
ATGTCGAAGATGAAGCACCTACTGCGAAAGCTTCACATCGGTGGCGGACTCAATGAGCATCAGAGACTCGCCGAGACCCGACCCGTGACGAGTCCGAACACGGATCTCAACCCGCTTGCTTCTTCTCCGGCCTCGTCCACGGGCTCCGCCACAATGGGGAGAATCGCCGCCGTCGAATCGGTGAGTGATCGGACGGCTGGGGGGGAGAGTGGTGTTAGCCGTAGCGGTAGCGGCAGCGGTAGTGGCGGGGGCGTGGATTTCAATTTCTTGGAGGAGGAGTTTCAGGTTCAGTTGGCCCTGGCGATCAGCGCTTCGGATCCCGATTCGCGCGATGACCCCGAGACGGCTCAGATCGACGCCGCTAAGCGGATTAGCCTCGGCTGCTCGGCTTCTACTGTCACTGACACTCAAGCTCCCTGCGACATGCTCTCGCTTCGCTACTGGAGCCACAATATTGTAGATTATAATGAAAAGGTTGTCGATGGATTTTATGACATGTATGGAATGACCTCAAATTTGCTTAGACAAGGGAAGATGCCATTGTTGGTGGATCTTCGAGCTGTATCTGTTTCAGAGAATGTTGATTATGATGTCATATTAGTGAACCGTTTGGTTGATCCCGATCTGCAACAACTTGAGAAAAGGGCATATGCTGTATCTGTAGAGTCACAGATTTCTCAACATGGTGTCCTTTTAAGCGGTTTAATTCAGAAAATTGCTGACATTGTTGTTGATAGAATGGGTGGTCCAGTTGCCGATGCTGATGAAATTTTGAGAAGGTGGAAAGTGAGAAGGTATGAGTTACGGAGTTCGATGAAAACTATCATTCTTCCCCTCGGACTTATTGATGTTGGACTTTCACGCCACAGGGCCCTGCTCTTTAAGGTACTGGCTGATAAGATAAACCTTCCATGTATGCTGGTCAAAGGTAGCTACTACACTGGCACTGATGATGGCGCTGTAAATTTGATTAAAATTGATAGTGCAAGTGAGTATATTATTGATCTGATGGGTGCACCTGGGACACTGATTCCTGCCGAGGTACCCACTAGTCAGCTCCCAAATTCTTTCTTTGCAATAAGGAGCTTTCAAGATGCCACCGAAATGCCCAAAGATGCGTGTTTGGCTCAAGCTGAGGGCAGTGGAATGTTAGTAGTTCCTCCCAATCTTGACAGAGTTTCTAGAGCTGGCAGCTCACGGTCAGAAGAAGCATCATATACAGGAGTTCAAACAAATGATGATAGAAGAATCATTGTTGACGAAAACCAAATGGAGAGTTCTGGCAATGGTATGGGAACTCCCCTCATATCACTTCCTAAATCATGTGAAAGTTCATCGGGTACAACTGAAAAAGCCACATCTGCGCAAAAAAGGAAGGTGAAAAATGTATCAAAGTATGTCATCAGTGCAGCAAAGAACCCAGAGTTTGCACAAAAACTACATGCCGTCTTGTTGGAAAGTGGTGCATCACCTCCTGCAGATTTATTTTCAGATATGAATCCTCAATATCTGCATGAAGACAAGTTGCTTGGTCAAATGAATGCAGATGGGAAACTTGTAGATGATGGGATTCATAATTATTTGGTACAATTAATATCAGGCAACGAGCAATCGGCTCAAACTTCTGCTGCAGTGGGCAACACCAATTTTAATAACCTGCTGAAACAATCTTCTGTGGAATTAGCTGAGCAACGAAATGAATCAGAGACAAACAAGTTTTTGCTTCCTTCAGATACTGGTGGAACTAGTGAAACAACCCAGATTAGTGCTAAAAATTCAGATCCTGCTCTTGTTAATCCCCCAAAAATGAATTCAGAAGCTTTTAACGAGGATAAAGAATCAGTAAGTAAACCAATAGACGCATGCAATAGTGGCCTGTGTACTAGTTGTGATAGTCACTATGAGAGGTACCCAGCACTGGGAGAAGTGGCAGAGTGGGAAATTCTATGGGAGGATCTTCAGATTGGTGAACGCATTGGCATTGGTTCCTATGGCGAGGTATACCATGCAGATTGGAATGGCACTGAAGTAGCTGTGAAAAAATTTTTAGATCAAGATTTTTCTGGTGATGCATTAGTTCAGTTTAAATGTGAAGTAGAAATTATGTTGAGACTTAGACATCCCAATGTTGTCCTTTTTATGGGGGCAGTTACTCGCCCTCCACATTTTTCTATCCTGACAGAGTTTCTTCCCAGGGGGAGTTTATATAGATTGCTGCATCGTCCCAATTCTCAACTTGATGAAAGGAGGCGAATGCGAATGGCCCTTGATGTGGCCAAGGGAATGAATTACCTGCATACTAGCCATCCTACTGTTGTGCATCGAGACCTAAAGTCTCCAAACCTCCTTGTTGATAAGAATTGGAATGTGAAGGTTTGTGACTTTGGGTTGTCGCGTACGAAGCACCATACTTATCTGTCATCTAAGTCTACCGCTGGAACGCCGGAATGGATGGCACCGGAGGTTTTGAGGAATGAACCAGCTAATGAGAAGTGTGATGTGTACAGCTTTGGCGTTATCTTATGGGAACTGGCTACTTGCTGTATCCCATGGAAAGGTTTGAACCCGATGCAGGTTGTTGGAGCAGTTGGATTCCAGAACAGACGTCTTGAAATTCCAGAGGACATTGATCCCGTGGTTGCCGAGATAATACGTGATTGTTGGCAAATAGAGCCAAATCTACGGCCGTCATTCTCGCAACTCATGGTCCGCCTGCGACGCCTTCAACGTTTTGTTGGAAGAACAAACTCTACAAATCAAATGACTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

924

Amino Acids

101.6

Weight (kDa)

5.46

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 141 - 344 1e-72 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 653 - 904 2.2e-65 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 654 - 902 1.2e-51 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 465
AatII GACGTC 1 cut(s) 2608
Acc16I TGCGCA 1 cut(s) 1365
Acc36I ACCTGC 3 cut(s) 1665, 2301, 2563
Acc65I GGTACC 2 cut(s) 1042, 1908
AccB1I GGYRCC 3 cut(s) 1042, 1908, 2455
AccI GTMKAC 2 cut(s) 2001, 2429
AccII CGCG 3 cut(s) 328, 330, 2395
AclI AACGTT 1 cut(s) 2728
AclWI GGATC 8 cut(s) 111, 311, 324, 558, 611, 1775, 1962, 2624
AcoI YGGCCR 2 cut(s) 2274, 2684
AcsI RAATTY 9 cut(s) 514, 768, 964, 1063, 1774, 1810, 1941, 2042, 2613
AcuI CTGAAG 3 cut(s) 1713, 1943, 2046
AcyI GRCGYC 3 cut(s) 357, 2605, 2719
AfaI GTAC 8 cut(s) 888, 1044, 1342, 1587, 1882, 1910, 2398, 2504
AfeI AGCGCT 1 cut(s) 310
AfiI CCNNNNNNNGG 9 cut(s) 156, 343, 366, 1918, 1919, 2140, 2272, 2555, 2572
AflIII ACRYGT 1 cut(s) 495
AgsI TTSAA 6 cut(s) 262, 1091, 1229, 2563, 2612, 2726
AhdI GACNNNNNGTC 1 cut(s) 2332
AhlI ACTAGT 3 cut(s) 1049, 1745, 1883
AjnI CCWGG 3 cut(s) 297, 1018, 2192
AjuI GAANNNNNNNTTGG 2 cut(s) 1795, 1827
AleI CACNNNNGTG 1 cut(s) 39
Alw21I GWGCWC 1 cut(s) 1018
Alw26I GTCTC 7 cut(s) 58, 68, 335, 1700, 2104, 2319, 2340
Alw44I GTGCAC 1 cut(s) 1014
AlwI GGATC 8 cut(s) 111, 311, 324, 558, 611, 1775, 1962, 2624
AlwNI CAGNNNCTG 2 cut(s) 1736, 1784
Ama87I CYCGRG 1 cut(s) 338
Aor51HI AGCGCT 1 cut(s) 310
AoxI GGCC 7 cut(s) 129, 294, 870, 1873, 2262, 2274, 2684
ApaI GGGCCC 1 cut(s) 874
ApaLI GTGCAC 1 cut(s) 1014
ApeKI GCWGC 6 cut(s) 234, 381, 1191, 1409, 1628, 2215
ApoI RAATTY 9 cut(s) 514, 768, 964, 1063, 1774, 1810, 1941, 2042, 2613
ArsI GACNNNNNNTTYG 2 cut(s) 499, 531
AseI ATTAAT 1 cut(s) 1592
Asp718I GGTACC 2 cut(s) 1042, 1908
AspLEI GCGC 4 cut(s) 311, 330, 959, 1366
AspS9I GGNCC 6 cut(s) 295, 746, 870, 871, 2263, 2707
AsuHPI GGTGA 5 cut(s) 193, 1390, 1461, 1979, 2078
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 2 cut(s) 746, 2707
BaeGI GKGCMC 2 cut(s) 874, 1018
BalI TGGCCA 1 cut(s) 2276
BamHI GGATCC 1 cut(s) 316
BanI GGYRCC 3 cut(s) 1042, 1908, 2455
BanII GRGCYC 2 cut(s) 146, 874
BbsI GAAGAC 1 cut(s) 1524
Bbv12I GWGCWC 1 cut(s) 1018
BbvCI CCTCAGC 1 cut(s) 1134
BbvI GCAGC 6 cut(s) 246, 368, 1203, 1421, 1615, 2202
BccI CCATC 7 cut(s) 476, 947, 1003, 1541, 1559, 2313, 2446
BceAI ACGGC 7 cut(s) 155, 204, 210, 360, 1430, 2671, 2699
BciT130I CCWGG 3 cut(s) 299, 1020, 2194
BciVI GTATCC 1 cut(s) 2555
BcoDI GTCTC 7 cut(s) 58, 68, 335, 1700, 2104, 2319, 2340
BcuI ACTAGT 3 cut(s) 1049, 1745, 1883
BfaI CTAG 5 cut(s) 1050, 1182, 1746, 1884, 2301
BfmI CTRYAG 3 cut(s) 660, 1476, 1629
BfoI RGCGCY 2 cut(s) 312, 960
BfuAI ACCTGC 3 cut(s) 1665, 2301, 2563
BfuI GTATCC 1 cut(s) 2555
BisI GCNGC 9 cut(s) 168, 232, 235, 360, 382, 1192, 1410, 1629, 2216
BlpI GCTNAGC 2 cut(s) 363, 1686
BlsI GCNGC 9 cut(s) 169, 233, 236, 361, 383, 1193, 1411, 1630, 2217
Bme1390I CCNGG 3 cut(s) 299, 1020, 2194
Bme18I GGWCC 2 cut(s) 746, 2707
BmeRI GACNNNNNGTC 1 cut(s) 2332
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 6 cut(s) 295, 746, 870, 871, 2263, 2707
BmiI GGNNCC 8 cut(s) 145, 318, 446, 872, 1044, 1910, 1987, 2457
BmrFI CCNGG 3 cut(s) 299, 1020, 2194
BmrI ACTGGG 1 cut(s) 1928
BmuI ACTGGG 1 cut(s) 1928
BpiI GAAGAC 1 cut(s) 1524
BpmI CTGGAG 1 cut(s) 463
Bpu10I CCTNAGC 1 cut(s) 1134
Bpu1102I GCTNAGC 2 cut(s) 363, 1686
BpuEI CTTGAG 3 cut(s) 393, 656, 1113
BsaAI YACGTR 1 cut(s) 2654
BsaBI GATNNNNATC 2 cut(s) 621, 1497
BsaHI GRCGYC 3 cut(s) 357, 2605, 2719
BsaI GGTCTC 2 cut(s) 68, 2319
BsaWI WCCGGW 1 cut(s) 2458
BsaXI ACNNNNNCTCC 6 cut(s) 263, 293, 397, 427, 2143, 2173
Bsc4I CCNNNNNNNGG 9 cut(s) 156, 343, 366, 1918, 1919, 2140, 2272, 2555, 2572
Bse1I ACTGG 7 cut(s) 446, 749, 894, 946, 1741, 1923, 2535
Bse3DI GCAATG 1 cut(s) 1291
Bse8I GATNNNNATC 2 cut(s) 621, 1497
BseBI CCWGG 3 cut(s) 299, 1020, 2194
BseGI GGATG 3 cut(s) 2119, 2305, 2457
BseJI GATNNNNATC 2 cut(s) 621, 1497
BseLI CCNNNNNNNGG 9 cut(s) 156, 343, 366, 1918, 1919, 2140, 2272, 2555, 2572
BseMI GCAATG 1 cut(s) 1291
BseMII CTCAG 3 cut(s) 362, 1125, 1677
BseNI ACTGG 7 cut(s) 446, 749, 894, 946, 1741, 1923, 2535
BseRI GAGGAG 2 cut(s) 287, 290
BseSI GKGCMC 2 cut(s) 874, 1018
BseX3I CGGCCG 1 cut(s) 2684
BseXI GCAGC 6 cut(s) 246, 368, 1203, 1421, 1615, 2202
BseYI CCCAGC 2 cut(s) 197, 1912
BsgI GTGCAG 1 cut(s) 1428
Bsh1236I CGCG 3 cut(s) 328, 330, 2395
Bsh1285I CGRYCG 1 cut(s) 2687
BshFI GGCC 7 cut(s) 131, 296, 872, 1875, 2264, 2276, 2686
BshNI GGYRCC 3 cut(s) 1042, 1908, 2455
BsiEI CGRYCG 1 cut(s) 2687
BsiHKAI GWGCWC 1 cut(s) 1018
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 3 cut(s) 128, 2444, 2459
BsiWI CGTACG 1 cut(s) 2396
BslFI GGGAC 2 cut(s) 1036, 2208
BslI CCNNNNNNNGG 9 cut(s) 156, 343, 366, 1918, 1919, 2140, 2272, 2555, 2572
BsmAI GTCTC 7 cut(s) 58, 68, 335, 1700, 2104, 2319, 2340
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 2 cut(s) 1036, 2208
BsmI GAATGC 2 cut(s) 1546, 2259
BsnI GGCC 7 cut(s) 131, 296, 872, 1875, 2264, 2276, 2686
Bso31I GGTCTC 2 cut(s) 68, 2319
BsoBI CYCGRG 1 cut(s) 338
Bsp120I GGGCCC 1 cut(s) 870
Bsp1286I GDGCHC 3 cut(s) 146, 874, 1018
Bsp1407I TGTACA 1 cut(s) 2502
Bsp1720I GCTNAGC 2 cut(s) 363, 1686
Bsp19I CCATGG 1 cut(s) 2549
BspANI GGCC 7 cut(s) 131, 296, 872, 1875, 2264, 2276, 2686
BspCNI CTCAG 3 cut(s) 361, 1126, 1678
BspFNI CGCG 3 cut(s) 328, 330, 2395
BspLI GGNNCC 8 cut(s) 145, 318, 446, 872, 1044, 1910, 1987, 2457
BspMAI CTGCAG 2 cut(s) 1480, 1633
BspMI ACCTGC 3 cut(s) 1665, 2301, 2563
BspPI GGATC 8 cut(s) 111, 311, 324, 558, 611, 1775, 1962, 2624
BspT107I GGYRCC 3 cut(s) 1042, 1908, 2455
BspTNI GGTCTC 2 cut(s) 68, 2319
BsrDI GCAATG 1 cut(s) 1291
BsrGI TGTACA 1 cut(s) 2502
BsrI ACTGG 7 cut(s) 446, 749, 894, 946, 1741, 1923, 2535
BssNAI GTATAC 1 cut(s) 2002
BssNI GRCGYC 3 cut(s) 357, 2605, 2719
BssT1I CCWWGG 2 cut(s) 2277, 2549
Bst1107I GTATAC 1 cut(s) 2002
Bst2UI CCWGG 3 cut(s) 299, 1020, 2194
Bst4CI ACNGT 4 cut(s) 397, 608, 1200, 2314
BstACI GRCGYC 3 cut(s) 357, 2605, 2719
BstAPI GCANNNNNTGC 1 cut(s) 22
BstAUI TGTACA 1 cut(s) 2502
BstBAI YACGTR 1 cut(s) 2654
BstC8I GCNNGC 4 cut(s) 117, 1189, 1864, 2714
BstDEI CTNAG 7 cut(s) 348, 363, 521, 1134, 1686, 2114, 2424
BstDSI CCRYGG 3 cut(s) 138, 2549, 2634
BstF5I GGATG 3 cut(s) 2119, 2305, 2457
BstFNI CGCG 3 cut(s) 328, 330, 2395
BstH2I RGCGCY 2 cut(s) 312, 960
BstHHI GCGC 4 cut(s) 311, 330, 959, 1366
BstMAI GTCTC 7 cut(s) 58, 68, 335, 1700, 2104, 2319, 2340
BstMCI CGRYCG 1 cut(s) 2687
BstNI CCWGG 3 cut(s) 299, 1020, 2194
BstNSI RCATGY 4 cut(s) 427, 499, 1445, 1866
BstSCI CCNGG 3 cut(s) 297, 1018, 2192
BstSFI CTRYAG 3 cut(s) 660, 1476, 1629
BstSLI GKGCMC 2 cut(s) 874, 1018
BstUI CGCG 3 cut(s) 328, 330, 2395
BstV1I GCAGC 6 cut(s) 246, 368, 1203, 1421, 1615, 2202
BstV2I GAAGAC 1 cut(s) 1524
BstX2I RGATCY 5 cut(s) 103, 316, 550, 1780, 1954
BstYI RGATCY 5 cut(s) 103, 316, 550, 1780, 1954
BstZ17I GTATAC 1 cut(s) 2002
BstZI CGGCCG 1 cut(s) 2684
BsuI GTATCC 1 cut(s) 2555
BsuRI GGCC 7 cut(s) 131, 296, 872, 1875, 2264, 2276, 2686
BtgI CCRYGG 3 cut(s) 138, 2549, 2634
BtgZI GCGATG 1 cut(s) 345
BtsCI GGATG 3 cut(s) 2119, 2305, 2457
BtsI GCAGTG 2 cut(s) 1147, 1638
BtsIMutI CAGTG 9 cut(s) 399, 939, 945, 1025, 1147, 1411, 1638, 1916, 2022
BveI ACCTGC 3 cut(s) 1665, 2301, 2563
Cac8I GCNNGC 4 cut(s) 117, 1189, 1864, 2714
CaiI CAGNNNCTG 2 cut(s) 1736, 1784
CfoI GCGC 4 cut(s) 311, 330, 959, 1366
Cfr13I GGNCC 6 cut(s) 295, 746, 870, 871, 2263, 2707
CseI GACGC 3 cut(s) 365, 1868, 2727
Csp6I GTAC 8 cut(s) 887, 1043, 1341, 1586, 1881, 1909, 2397, 2503
CviQI GTAC 8 cut(s) 887, 1043, 1341, 1586, 1881, 1909, 2397, 2503
DdeI CTNAG 7 cut(s) 348, 363, 521, 1134, 1686, 2114, 2424
DraI TTTAAA 1 cut(s) 2086
DriI GACNNNNNGTC 1 cut(s) 2332
EaeI YGGCCR 2 cut(s) 2274, 2684
EagI CGGCCG 1 cut(s) 2684
Eam1105I GACNNNNNGTC 1 cut(s) 2332
EciI GGCGGA 3 cut(s) 60, 136, 2699
EclXI CGGCCG 1 cut(s) 2684
Eco130I CCWWGG 2 cut(s) 2277, 2549
Eco24I GRGCYC 2 cut(s) 146, 874
Eco31I GGTCTC 2 cut(s) 68, 2319
Eco47I GGWCC 2 cut(s) 746, 2707
Eco47III AGCGCT 1 cut(s) 310
Eco52I CGGCCG 1 cut(s) 2684
Eco57I CTGAAG 3 cut(s) 1713, 1943, 2046
Eco88I CYCGRG 1 cut(s) 338
EcoO109I RGGNCCY 2 cut(s) 870, 871
EcoRI GAATTC 1 cut(s) 1810
EcoRII CCWGG 3 cut(s) 297, 1018, 2192
EcoT14I CCWWGG 2 cut(s) 2277, 2549
EcoT22I ATGCAT 1 cut(s) 2074
EcoT38I GRGCYC 2 cut(s) 146, 874
ErhI CCWWGG 2 cut(s) 2277, 2549
Esp3I CGTCTC 1 cut(s) 335
FaqI GGGAC 2 cut(s) 1036, 2208
FauI CCCGC 2 cut(s) 120, 239
FauNDI CATATG 1 cut(s) 649
FblI GTMKAC 2 cut(s) 2001, 2429
Fnu4HI GCNGC 9 cut(s) 168, 232, 235, 360, 382, 1192, 1410, 1629, 2216
FokI GGATG 3 cut(s) 2106, 2292, 2464
FriOI GRGCYC 2 cut(s) 146, 874
Fsp4HI GCNGC 9 cut(s) 168, 232, 235, 360, 382, 1192, 1410, 1629, 2216
FspBI CTAG 5 cut(s) 1050, 1182, 1746, 1884, 2301
FspI TGCGCA 1 cut(s) 1365
GlaI GCGC 4 cut(s) 310, 329, 958, 1365
GluI GCNGC 9 cut(s) 168, 232, 235, 360, 382, 1192, 1410, 1629, 2216
GsaI CCCAGC 2 cut(s) 201, 1916
GsuI CTGGAG 1 cut(s) 463
HaeII RGCGCY 2 cut(s) 312, 960
HaeIII GGCC 7 cut(s) 131, 296, 872, 1875, 2264, 2276, 2686
HapII CCGG 3 cut(s) 128, 2444, 2459
HgaI GACGC 3 cut(s) 365, 1868, 2727
HhaI GCGC 4 cut(s) 311, 330, 959, 1366
Hin1I GRCGYC 3 cut(s) 357, 2605, 2719
Hin6I GCGC 4 cut(s) 309, 328, 957, 1364
HinP1I GCGC 4 cut(s) 309, 328, 957, 1364
HincII GTYRAC 1 cut(s) 1258
HindII GTYRAC 1 cut(s) 1258
HindIII AAGCTT 2 cut(s) 29, 1818
HpaII CCGG 3 cut(s) 128, 2444, 2459
HphI GGTGA 5 cut(s) 193, 1390, 1461, 1979, 2078
Hpy99I CGWCG 3 cut(s) 176, 359, 2721
HpyAV CCTTC 7 cut(s) 771, 786, 917, 1369, 1737, 2365, 2732
HpyCH4III ACNGT 4 cut(s) 397, 608, 1200, 2314
HpyCH4IV ACGT 3 cut(s) 2605, 2653, 2728
HpyF3I CTNAG 7 cut(s) 348, 363, 521, 1134, 1686, 2114, 2424
HpySE526I ACGT 3 cut(s) 2605, 2653, 2728
Hsp92I GRCGYC 3 cut(s) 357, 2605, 2719
HspAI GCGC 4 cut(s) 309, 328, 957, 1364
KpnI GGTACC 2 cut(s) 1046, 1912
LmnI GCTCC 6 cut(s) 149, 418, 444, 1062, 1083, 2582
Lsp1109I GCAGC 6 cut(s) 246, 368, 1203, 1421, 1615, 2202
MaeI CTAG 5 cut(s) 1050, 1182, 1746, 1884, 2301
MaeII ACGT 3 cut(s) 2605, 2653, 2728
MaeIII GTNAC 7 cut(s) 85, 397, 666, 801, 1895, 2149, 2378
MflI RGATCY 5 cut(s) 103, 316, 550, 1780, 1954
MhlI GDGCHC 3 cut(s) 146, 874, 1018
MlsI TGGCCA 1 cut(s) 2276
MluNI TGGCCA 1 cut(s) 2276
MlyI GAGTC 4 cut(s) 42, 60, 100, 674
MmeI TCCRAC 5 cut(s) 832, 1434, 2560, 2569, 2716
Mox20I TGGCCA 1 cut(s) 2276
Mph1103I ATGCAT 1 cut(s) 2074
MscI TGGCCA 1 cut(s) 2276
MseI TTAA 9 cut(s) 695, 704, 882, 972, 1592, 1650, 1794, 1824, 2085
MslI CAYNNNNRTG 4 cut(s) 39, 599, 1103, 2124
Msp20I TGGCCA 1 cut(s) 2276
MspA1I CMGCKG 2 cut(s) 237, 2435
MspI CCGG 3 cut(s) 128, 2444, 2459
MspR9I CCNGG 3 cut(s) 299, 1020, 2194
Mva1269I GAATGC 2 cut(s) 1546, 2259
MvaI CCWGG 3 cut(s) 299, 1020, 2194
MvnI CGCG 3 cut(s) 328, 330, 2395
NcoI CCATGG 1 cut(s) 2549
NdeI CATATG 1 cut(s) 649
NlaIV GGNNCC 8 cut(s) 145, 318, 446, 872, 1044, 1910, 1987, 2457
NmeAIII GCCGAG 5 cut(s) 97, 357, 365, 1063, 2668
NmuCI GTSAC 5 cut(s) 85, 397, 666, 1895, 2378
NsbI TGCGCA 1 cut(s) 1365
NsiI ATGCAT 1 cut(s) 2074
NspI RCATGY 4 cut(s) 427, 499, 1445, 1866
OliI CACNNNNGTG 1 cut(s) 39
PaeI GCATGC 1 cut(s) 1866
PasI CCCWGGG 1 cut(s) 2193
PciI ACATGT 1 cut(s) 495
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 2725
PctI GAATGC 2 cut(s) 1546, 2259
Pfl23II CGTACG 1 cut(s) 2396
PkrI GCNGC 9 cut(s) 169, 233, 236, 361, 383, 1193, 1411, 1630, 2217
PleI GAGTC 4 cut(s) 42, 60, 99, 673
PpsI GAGTC 4 cut(s) 42, 60, 99, 673
Ppu21I YACGTR 1 cut(s) 2654
PscI ACATGT 1 cut(s) 495
PshBI ATTAAT 1 cut(s) 1592
PsiI TTATAA 1 cut(s) 465
Psp1406I AACGTT 1 cut(s) 2728
Psp6I CCWGG 3 cut(s) 297, 1018, 2192
PspFI CCCAGC 2 cut(s) 197, 1912
PspGI CCWGG 3 cut(s) 297, 1018, 2192
PspLI CGTACG 1 cut(s) 2396
PspN4I GGNNCC 8 cut(s) 145, 318, 446, 872, 1044, 1910, 1987, 2457
PspOMI GGGCCC 1 cut(s) 870
PspPI GGNCC 6 cut(s) 295, 746, 870, 871, 2263, 2707
PsrI GAACNNNNNNTAC 2 cut(s) 2734, 2766
PstI CTGCAG 2 cut(s) 1480, 1633
PstNI CAGNNNCTG 2 cut(s) 1736, 1784
PsuI RGATCY 5 cut(s) 103, 316, 550, 1780, 1954
RsaI GTAC 8 cut(s) 888, 1044, 1342, 1587, 1882, 1910, 2398, 2504
RsaNI GTAC 8 cut(s) 887, 1043, 1341, 1586, 1881, 1909, 2397, 2503
RseI CAYNNNNRTG 4 cut(s) 39, 599, 1103, 2124
SaqAI TTAA 9 cut(s) 695, 704, 882, 972, 1592, 1650, 1794, 1824, 2085
SatI GCNGC 9 cut(s) 168, 232, 235, 360, 382, 1192, 1410, 1629, 2216
Sau96I GGNCC 6 cut(s) 295, 746, 870, 871, 2263, 2707
SchI GAGTC 4 cut(s) 42, 60, 100, 674
ScrFI CCNGG 3 cut(s) 299, 1020, 2194
SduI GDGCHC 3 cut(s) 146, 874, 1018
SfcI CTRYAG 3 cut(s) 660, 1476, 1629
SinI GGWCC 2 cut(s) 746, 2707
SmiMI CAYNNNNRTG 4 cut(s) 39, 599, 1103, 2124
SmlI CTYRAG 3 cut(s) 408, 635, 1128
SmoI CTYRAG 3 cut(s) 408, 635, 1128
SpeI ACTAGT 3 cut(s) 1049, 1745, 1883
SphI GCATGC 1 cut(s) 1866
SspI AATATT 1 cut(s) 454
SspMI CTAG 5 cut(s) 1050, 1182, 1746, 1884, 2301
StyD4I CCNGG 3 cut(s) 297, 1018, 2192
StyI CCWWGG 2 cut(s) 2277, 2549
TaaI ACNGT 4 cut(s) 397, 608, 1200, 2314
TaiI ACGT 3 cut(s) 2608, 2656, 2731
TaqI TCGA 7 cut(s) 5, 174, 354, 480, 556, 812, 2323
TatI WGTACW 2 cut(s) 1880, 2502
TauI GCSGC 3 cut(s) 170, 234, 362
Tru1I TTAA 9 cut(s) 695, 704, 882, 972, 1592, 1650, 1794, 1824, 2085
Tru9I TTAA 9 cut(s) 695, 704, 882, 972, 1592, 1650, 1794, 1824, 2085
TscAI CASTG 9 cut(s) 406, 946, 952, 1032, 1147, 1411, 1638, 1923, 2029
TseFI GTSAC 5 cut(s) 85, 397, 666, 1895, 2378
TseI GCWGC 6 cut(s) 234, 381, 1191, 1409, 1628, 2215
Tsp45I GTSAC 5 cut(s) 85, 397, 666, 1895, 2378
TspGWI ACGGA 2 cut(s) 116, 820
TspRI CASTG 9 cut(s) 406, 946, 952, 1032, 1147, 1411, 1638, 1923, 2029
VneI GTGCAC 1 cut(s) 1014
VpaK11BI GGWCC 2 cut(s) 746, 2707
VspI ATTAAT 1 cut(s) 1592
XapI RAATTY 9 cut(s) 514, 768, 964, 1063, 1774, 1810, 1941, 2042, 2613
XbaI TCTAGA 1 cut(s) 1181
XceI RCATGY 4 cut(s) 427, 499, 1445, 1866
XmiI GTMKAC 2 cut(s) 2001, 2429
XspI CTAG 5 cut(s) 1050, 1182, 1746, 1884, 2301
ZraI GACGTC 1 cut(s) 2606
Zsp2I ATGCAT 1 cut(s) 2074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.