Rw6G043340

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0006217
Physical Location & Seq
Reverse (-)
66690943 .. 66696168
5226 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G043340.1

Sequence Viewer

Length: 2799 bp
ATGTCGAAGATGAAGCACCTACTGAGAAAGCTCCACATCGGCGGGGACCAGAGGCTGGCGGAGACGACGGCCGCGGTGGCCCCGCCGGTGGTGGCGAGTCCGGGGACGGCGAATCTCAACACGACGGCGTCGTCACCGGCGTCGTCATCGGGGTCGGCGACAATGGGGAGAATCGCGGCGGTGGAGTCGGTGAGTGATCGGACGTCTGGCGACGGCGGGGGGAGCGGGAGTGGCGGAGGAGGCGTGGATTTCAATTTCTTGGAGGAGGAGTTTCAGGTACAGTTGGCCCTAGCGATTAGCGCTTCCGATCCCGATTCGCGCGACGACCCTGAGACGGCTCAGATCGACGCCGCGAAACGGATTAGCCTCGGCTGCGGGGCTACGTCGCTCGCCGACGCACAAGCCCCTTTTCAGATGCTTTCGCTTCGCTACTGGAGCCATAATGTTGTAGATTATAATGAAAAAGTGGTGGATGGATTTTACGATGTGTATGGAATTACCTCGAATTCATTTAGACAAGGGAAGATGCCATTGTTGGTGGAATTTCGAGCGGTATCGGTTTCAGATAATGTCGACTATGATGTCATATTAGTTAACCGTTTTGTTGATACTGAACTGCAACAACTTGAGAAAAGAGCATATGCTGCATCTCTGGAGTCGGGGATTTCTCAACATGGTCTACTTTTAAGTGGCTTGATTCAGAAGATTGCTGATATTGTTGTTGATAGAATGGGTGGTCCAGTTGGAGATGCGGATGAGATTTTAAGAAGGTGGAAAATAAGGAGGCATGAGTTGCGGAGTTCAATGAACACTATCATTCTTCCCCTCGGAAAAATTGATGTTGGACTTTCACGCCACCGGGCCCTGCTCTTTAAGGTATTAGCTGATAAGATAAATCTTCCATGTATGCTGGTCAAAGGTAGCTACTACACTGGTACTGATGATGGAGCTGTAAACTTGATTAAAATTGATAGTGGAATTGGAAGCGAATATATTATTGATCTGATGGGTGCTCCTGGAACACTAATTCCTGCTGAGGTACCTACTAGTCAGCTCCCAAATTCTTTTTTTGCCATAAGGAGCTTTCAAGATCCCACAGAAATGCCTACAGAGTTGCCCAAAGATCTGTGTTTACTACAAGCTGAGGGAACTGGAATGTCGGCAGCTCCACCTGGTCTTGAAAGAGCTTACACATTTGGCAGCTCAAGGTCAGAAGAAGCATCATATGCCGGTGTTCAAACAAAAGATGACCAAAGAAGCGTCGTGGATGAAAATCAAATTGAGATTCTTAAAAGTGAGCTTGAGACTCCTCTTAAAAGTAAATCATGTGGAAGTTCATCAGGCCCATCTGGGAAAGCTACATCTGCACAGAAAAGAAAAGTGAAAAATGTATCAAGGTATGTCATCAGTGCCGCAAAGAACCCAGAATTTGCACAGAAACTACATGCTGTCTTGTTGGAGAGCGGTGCATCACCTCCTCCCGACTTATTTTCAGATATGAATCCTCAATACCTGAATGAAGGTAAATTGCTTGGACAAATCCATGCAAATGGGGAACTTGTAGATGAGGGGGTTCATGATTATCTGGTTAAACTATTGTCAAACAGTGATCAATCCTCTGCCGTGGATGTAGCTGAGCAACGGAATGAGTGGAGGACCAATTCTTTACTCTCTGATAATGTTGATGAGGGATTTGTAATGGTTTCTGGCAGAACTAGTGAAACAACCCAGATTGGTGCTATAAATTTAGATCCTGCTTTTGGTAATCCCCCAAGAATGAATGCAGAAGCTTTTCATGAGGAGAAAATTCATGATATATCCATGGTCTCTGGAACAACTTCTGCTAACACTCAATTGGGCAAAGAATCTGTTGCTCAATCAACGCAAACAGCCAATAGCAGTCTGAATGCTGCTTGGGATAGTCATGTTGATAGGTACCCAGCGTTGGGAGAAGTTGCGGAGTGGGAAATTCTATGGGAGGATCTTCAGATTGGAGAGCGCATTGGCATTGGTTCATATGGTGAGGTATATCATGCAGATTGGAATGGCACTGAAGTAGCTGTGAAGAAATTTTTAGATCAAGATTTTTCGGGTGATGCATTGGTTCAGTTTAGATGTGAAGTAGAGATCATGTTAAGACTTCGACATCCAAATGTTGTGCTTTTCATGGGCGCTGTTACTCGCCCTCCCCATTTCTCCATCCTGACAGAGTTTCTTCCTAGGGGTAGCTTATACCGATTACTGCATCGTCCAAATTCTCAACTTGATGAAAAGAGGCGAATGCGAATGGCTCTTGATGTGGCGAAGGGAATGAATTACCTCCACACCAGCAATCCTACAGTTGTGCATCGAGACCTTAAGTCTCCAAATCTCCTTGTTGATAAGAATTGGAATGTGAAGGTTTGTGACTTTGGGTTGTCACGTACGAAGCACCACACTTATCTGTCCTCAAAGTCCACTGCTGGAACGCCGGAATGGATGGCTCCTGAAGTTTTAAGGAATGAATTAGCTAACGAGAAGTGTGATGTGTACAGTTTTGGTGTGATATTATGGGAATTGACTACTTGCTGTATCCCATGGAAAGGATTGAACCCAATGCAGGTTGTTGGAGCTGTTGGATTCCAAAATAGGCGTCTTGAAATTCCGGATGACGTTGACCCAGTGGTTGCCGAGATAATACGTGATTGTTGGCAAACAGAGCCAAATCTACGGCCTTCTTTCTCCCAGCTCATGGTCCGACTCAAGCGTCTTCAACGGCTTGTTGGAAGAACGAACTCTGCAAATCAAATGACTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

932

Amino Acids

102.17

Weight (kDa)

5.49

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 138 - 343 6.5e-71 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 662 - 913 4.6e-66 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 663 - 911 2e-51 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 456
AasI GACNNNNNNGTC 3 cut(s) 130, 581, 2745
AatII GACGTC 1 cut(s) 206
Acc36I ACCTGC 1 cut(s) 2590
Acc65I GGTACC 2 cut(s) 1039, 1935
AccB1I GGYRCC 2 cut(s) 1039, 1935
AccB7I CCANNNNNTGG 2 cut(s) 55, 2731
AccBSI CCGCTC 3 cut(s) 225, 551, 1464
AccI GTMKAC 2 cut(s) 573, 679
AccII CGCG 5 cut(s) 74, 176, 319, 321, 353
AccIII TCCGGA 1 cut(s) 2644
AclWI GGATC 4 cut(s) 302, 1085, 1745, 1989
AcoI YGGCCR 1 cut(s) 69
AcuI CTGAAG 3 cut(s) 1970, 2073, 2508
AcyI GRCGYC 5 cut(s) 128, 140, 203, 348, 2632
AfaI GTAC 6 cut(s) 279, 937, 1041, 1937, 2425, 2531
AfeI AGCGCT 1 cut(s) 301
AflII CTTAAG 1 cut(s) 2357
AgsI TTSAA 8 cut(s) 253, 804, 1088, 1181, 1238, 2590, 2639, 2753
AhdI GACNNNNNGTC 1 cut(s) 2359
AhlI ACTAGT 2 cut(s) 1046, 1715
AjnI CCWGG 2 cut(s) 1015, 1171
AloI GAACNNNNNNTCC 2 cut(s) 1012, 1044
Alw21I GWGCWC 1 cut(s) 1015
Alw26I GTCTC 6 cut(s) 56, 326, 1298, 1831, 2346, 2367
AlwI GGATC 4 cut(s) 302, 1085, 1745, 1989
AlwNI CAGNNNCTG 1 cut(s) 55
Aor13HI TCCGGA 1 cut(s) 2644
Aor51HI AGCGCT 1 cut(s) 301
AoxI GGCC 6 cut(s) 69, 78, 285, 861, 1342, 2711
ApaI GGGCCC 1 cut(s) 865
ApeKI GCWGC 5 cut(s) 372, 644, 1163, 1200, 1910
ArsI GACNNNNNNTTYG 2 cut(s) 2617, 2649
Asp700I GAANNNNTTC 2 cut(s) 1791, 1837
Asp718I GGTACC 2 cut(s) 1039, 1935
AspA2I CCTAGG 1 cut(s) 2219
AspLEI GCGC 4 cut(s) 302, 321, 2001, 2174
AspS9I GGNCC 9 cut(s) 46, 79, 286, 737, 861, 862, 1343, 1656, 2734
AsuC2I CCSGG 2 cut(s) 102, 860
AsuHPI GGTGA 5 cut(s) 126, 202, 1464, 2033, 2105
AvaII GGWCC 4 cut(s) 46, 737, 1656, 2734
AvrII CCTAGG 1 cut(s) 2219
BaeGI GKGCMC 1 cut(s) 865
BanI GGYRCC 2 cut(s) 1039, 1935
BanII GRGCYC 1 cut(s) 865
BbsI GAAGAC 1 cut(s) 2741
Bbv12I GWGCWC 1 cut(s) 1015
BbvCI CCTCAGC 2 cut(s) 1035, 1143
BbvI GCAGC 5 cut(s) 359, 631, 1175, 1212, 1897
BccI CCATC 6 cut(s) 467, 938, 1000, 1354, 2207, 2473
BceAI ACGGC 8 cut(s) 84, 123, 141, 229, 351, 1607, 2726, 2771
BciT130I CCWGG 2 cut(s) 1017, 1173
BciVI GTATCC 1 cut(s) 2582
BclI TGATCA 1 cut(s) 1609
BcnI CCSGG 2 cut(s) 102, 860
BcoDI GTCTC 6 cut(s) 56, 326, 1298, 1831, 2346, 2367
BcuI ACTAGT 2 cut(s) 1046, 1715
BfaI CTAG 4 cut(s) 290, 1047, 1716, 2220
BfmI CTRYAG 2 cut(s) 1107, 2337
BfoI RGCGCY 2 cut(s) 303, 2175
BfrI CTTAAG 1 cut(s) 2357
BfuAI ACCTGC 1 cut(s) 2590
BfuI GTATCC 1 cut(s) 2582
BglI GCCNNNNNGGC 1 cut(s) 77
BglII AGATCT 1 cut(s) 1123
BisI GCNGC 9 cut(s) 72, 177, 351, 373, 645, 1164, 1201, 1413, 1911
BlnI CCTAGG 1 cut(s) 2219
BlpI GCTNAGC 1 cut(s) 1635
BlsI GCNGC 9 cut(s) 73, 178, 352, 374, 646, 1165, 1202, 1414, 1912
Bme1390I CCNGG 4 cut(s) 102, 860, 1017, 1173
Bme18I GGWCC 4 cut(s) 46, 737, 1656, 2734
BmeRI GACNNNNNGTC 1 cut(s) 2359
BmgT120I GGNCC 9 cut(s) 46, 79, 286, 737, 861, 862, 1343, 1656, 2734
BmiI GGNNCC 7 cut(s) 47, 81, 437, 863, 1041, 1937, 2484
BmrFI CCNGG 4 cut(s) 102, 860, 1017, 1173
BmrI ACTGGG 1 cut(s) 2654
BmsI GCATC 9 cut(s) 405, 516, 656, 739, 1229, 1478, 2086, 2254, 2356
BmuI ACTGGG 1 cut(s) 2654
BpiI GAAGAC 1 cut(s) 2741
BpmI CTGGAG 2 cut(s) 454, 674
Bpu10I CCTNAGC 2 cut(s) 1035, 1143
Bpu1102I GCTNAGC 1 cut(s) 1635
BpuEI CTTGAG 4 cut(s) 647, 1189, 1322, 2726
BpuMI CCSGG 2 cut(s) 102, 860
BsaAI YACGTR 2 cut(s) 2423, 2681
BsaBI GATNNNNATC 2 cut(s) 1272, 1500
BsaHI GRCGYC 5 cut(s) 128, 140, 203, 348, 2632
BsaI GGTCTC 2 cut(s) 1831, 2346
BsaJI CCNNGG 8 cut(s) 72, 101, 367, 826, 1623, 1821, 2219, 2576
BsaWI WCCGGW 1 cut(s) 2644
BsaXI ACNNNNNCTCC 8 cut(s) 214, 228, 244, 254, 258, 284, 2170, 2200
Bse118I RCCGGY 3 cut(s) 85, 136, 1229
Bse1I ACTGG 5 cut(s) 437, 740, 937, 1156, 2660
Bse8I GATNNNNATC 2 cut(s) 1272, 1500
BseAI TCCGGA 1 cut(s) 2644
BseBI CCWGG 2 cut(s) 1017, 1173
BseDI CCNNGG 8 cut(s) 72, 101, 367, 826, 1623, 1821, 2219, 2576
BseGI GGATG 8 cut(s) 478, 760, 1273, 1633, 2146, 2199, 2484, 2653
BseJI GATNNNNATC 2 cut(s) 1272, 1500
BseMII CTCAG 6 cut(s) 14, 321, 353, 1026, 1134, 1626
BseNI ACTGG 5 cut(s) 437, 740, 937, 1156, 2660
BseRI GAGGAG 6 cut(s) 252, 278, 281, 1299, 1467, 1814
BseSI GKGCMC 1 cut(s) 865
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 5 cut(s) 359, 631, 1175, 1212, 1897
BseYI CCCAGC 2 cut(s) 1939, 2724
BsgI GTGCAG 1 cut(s) 1350
Bsh1236I CGCG 5 cut(s) 74, 176, 319, 321, 353
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 6 cut(s) 71, 80, 287, 863, 1344, 2713
BshNI GGYRCC 2 cut(s) 1039, 1935
BsiEI CGRYCG 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 1015
BsiSI CCGG 7 cut(s) 86, 101, 137, 859, 1230, 2471, 2645
BsiWI CGTACG 1 cut(s) 2423
BslFI GGGAC 2 cut(s) 59, 118
BsmAI GTCTC 6 cut(s) 56, 326, 1298, 1831, 2346, 2367
BsmBI CGTCTC 2 cut(s) 56, 326
BsmFI GGGAC 2 cut(s) 59, 118
BsmI GAATGC 3 cut(s) 1786, 1912, 2286
BsnI GGCC 6 cut(s) 71, 80, 287, 863, 1344, 2713
Bso31I GGTCTC 2 cut(s) 1831, 2346
Bsp120I GGGCCC 1 cut(s) 861
Bsp1286I GDGCHC 2 cut(s) 865, 1015
Bsp13I TCCGGA 1 cut(s) 2644
Bsp1407I TGTACA 1 cut(s) 2529
Bsp1720I GCTNAGC 1 cut(s) 1635
Bsp19I CCATGG 2 cut(s) 1821, 2576
BspANI GGCC 6 cut(s) 71, 80, 287, 863, 1344, 2713
BspCNI CTCAG 6 cut(s) 15, 322, 352, 1027, 1135, 1627
BspEI TCCGGA 1 cut(s) 2644
BspFNI CGCG 5 cut(s) 74, 176, 319, 321, 353
BspHI TCATGA 3 cut(s) 1576, 1795, 1810
BspLI GGNNCC 7 cut(s) 47, 81, 437, 863, 1041, 1937, 2484
BspMI ACCTGC 1 cut(s) 2590
BspPI GGATC 4 cut(s) 302, 1085, 1745, 1989
BspT107I GGYRCC 2 cut(s) 1039, 1935
BspTI CTTAAG 1 cut(s) 2357
BspTNI GGTCTC 2 cut(s) 1831, 2346
BsrBI CCGCTC 3 cut(s) 225, 551, 1464
BsrFI RCCGGY 3 cut(s) 85, 136, 1229
BsrGI TGTACA 1 cut(s) 2529
BsrI ACTGG 5 cut(s) 437, 740, 937, 1156, 2660
BssAI RCCGGY 3 cut(s) 85, 136, 1229
BssECI CCNNGG 8 cut(s) 72, 101, 367, 826, 1623, 1821, 2219, 2576
BssNI GRCGYC 5 cut(s) 128, 140, 203, 348, 2632
BssT1I CCWWGG 3 cut(s) 1821, 2219, 2576
Bst2UI CCWGG 2 cut(s) 1017, 1173
Bst4CI ACNGT 5 cut(s) 282, 599, 1607, 2341, 2534
BstACI GRCGYC 5 cut(s) 128, 140, 203, 348, 2632
BstAFI CTTAAG 1 cut(s) 2357
BstAPI GCANNNNNTGC 3 cut(s) 644, 793, 1226
BstAUI TGTACA 1 cut(s) 2529
BstBAI YACGTR 2 cut(s) 2423, 2681
BstC8I GCNNGC 2 cut(s) 57, 390
BstDEI CTNAG 6 cut(s) 23, 330, 339, 1035, 1143, 1635
BstDSI CCRYGG 4 cut(s) 72, 1623, 1821, 2576
BstF5I GGATG 8 cut(s) 478, 760, 1273, 1633, 2146, 2199, 2484, 2653
BstFNI CGCG 5 cut(s) 74, 176, 319, 321, 353
BstH2I RGCGCY 2 cut(s) 303, 2175
BstHHI GCGC 4 cut(s) 302, 321, 2001, 2174
BstMAI GTCTC 6 cut(s) 56, 326, 1298, 1831, 2346, 2367
BstMCI CGRYCG 1 cut(s) 72
BstNI CCWGG 2 cut(s) 1017, 1173
BstNSI RCATGY 1 cut(s) 1448
BstSCI CCNGG 4 cut(s) 100, 858, 1015, 1171
BstSFI CTRYAG 2 cut(s) 1107, 2337
BstSLI GKGCMC 1 cut(s) 865
BstUI CGCG 5 cut(s) 74, 176, 319, 321, 353
BstV1I GCAGC 5 cut(s) 359, 631, 1175, 1212, 1897
BstV2I GAAGAC 1 cut(s) 2741
BstX2I RGATCY 4 cut(s) 1090, 1123, 1750, 1981
BstXI CCANNNNNNTGG 1 cut(s) 1550
BstYI RGATCY 4 cut(s) 1090, 1123, 1750, 1981
BstZI CGGCCG 1 cut(s) 69
BsuI GTATCC 1 cut(s) 2582
BsuRI GGCC 6 cut(s) 71, 80, 287, 863, 1344, 2713
BtgI CCRYGG 4 cut(s) 72, 1623, 1821, 2576
BtsCI GGATG 8 cut(s) 478, 760, 1273, 1633, 2146, 2199, 2484, 2653
BtsI GCAGTG 1 cut(s) 2457
BtsIMutI CAGTG 6 cut(s) 930, 1414, 1612, 2049, 2457, 2667
BveI ACCTGC 1 cut(s) 2590
Cac8I GCNNGC 2 cut(s) 57, 390
CaiI CAGNNNCTG 1 cut(s) 55
CciI TCATGA 3 cut(s) 1576, 1795, 1810
CfoI GCGC 4 cut(s) 302, 321, 2001, 2174
Cfr10I RCCGGY 3 cut(s) 85, 136, 1229
Cfr13I GGNCC 9 cut(s) 46, 79, 286, 737, 861, 862, 1343, 1656, 2734
Cfr42I CCGCGG 1 cut(s) 75
CseI GACGC 7 cut(s) 117, 129, 356, 404, 1249, 2621, 2735
CsiI ACCWGGT 1 cut(s) 1171
Csp6I GTAC 6 cut(s) 278, 936, 1040, 1936, 2424, 2530
CviQI GTAC 6 cut(s) 278, 936, 1040, 1936, 2424, 2530
DdeI CTNAG 6 cut(s) 23, 330, 339, 1035, 1143, 1635
DrdI GACNNNNNNGTC 3 cut(s) 130, 581, 2745
DriI GACNNNNNGTC 1 cut(s) 2359
DseDI GACNNNNNNGTC 3 cut(s) 130, 581, 2745
EaeI YGGCCR 1 cut(s) 69
EagI CGGCCG 1 cut(s) 69
Eam1105I GACNNNNNGTC 1 cut(s) 2359
EciI GGCGGA 2 cut(s) 74, 249
EclXI CGGCCG 1 cut(s) 69
Eco130I CCWWGG 3 cut(s) 1821, 2219, 2576
Eco24I GRGCYC 1 cut(s) 865
Eco31I GGTCTC 2 cut(s) 1831, 2346
Eco47I GGWCC 4 cut(s) 46, 737, 1656, 2734
Eco47III AGCGCT 1 cut(s) 301
Eco52I CGGCCG 1 cut(s) 69
Eco57I CTGAAG 3 cut(s) 1970, 2073, 2508
EcoO109I RGGNCCY 1 cut(s) 862
EcoRI GAATTC 1 cut(s) 505
EcoRII CCWGG 2 cut(s) 1015, 1171
EcoT14I CCWWGG 3 cut(s) 1821, 2219, 2576
EcoT22I ATGCAT 1 cut(s) 2101
EcoT38I GRGCYC 1 cut(s) 865
ErhI CCWWGG 3 cut(s) 1821, 2219, 2576
Esp3I CGTCTC 2 cut(s) 56, 326
FaqI GGGAC 2 cut(s) 59, 118
FauI CCCGC 5 cut(s) 35, 90, 209, 218, 368
FauNDI CATATG 3 cut(s) 640, 1225, 2017
FbaI TGATCA 1 cut(s) 1609
FblI GTMKAC 2 cut(s) 573, 679
Fnu4HI GCNGC 9 cut(s) 72, 177, 351, 373, 645, 1164, 1201, 1413, 1911
FokI GGATG 8 cut(s) 485, 767, 1280, 1640, 2133, 2186, 2491, 2660
FriOI GRGCYC 1 cut(s) 865
Fsp4HI GCNGC 9 cut(s) 72, 177, 351, 373, 645, 1164, 1201, 1413, 1911
FspBI CTAG 4 cut(s) 290, 1047, 1716, 2220
GlaI GCGC 4 cut(s) 301, 320, 2000, 2173
GluI GCNGC 9 cut(s) 72, 177, 351, 373, 645, 1164, 1201, 1413, 1911
GsaI CCCAGC 2 cut(s) 1943, 2728
GsuI CTGGAG 2 cut(s) 454, 674
HaeII RGCGCY 2 cut(s) 303, 2175
HaeIII GGCC 6 cut(s) 71, 80, 287, 863, 1344, 2713
HapII CCGG 7 cut(s) 86, 101, 137, 859, 1230, 2471, 2645
HgaI GACGC 7 cut(s) 117, 129, 356, 404, 1249, 2621, 2735
HhaI GCGC 4 cut(s) 302, 321, 2001, 2174
Hin1I GRCGYC 5 cut(s) 128, 140, 203, 348, 2632
Hin6I GCGC 4 cut(s) 300, 319, 1999, 2172
HinP1I GCGC 4 cut(s) 300, 319, 1999, 2172
HincII GTYRAC 3 cut(s) 574, 595, 2656
HindII GTYRAC 3 cut(s) 574, 595, 2656
HindIII AAGCTT 1 cut(s) 1788
HpaI GTTAAC 1 cut(s) 595
HpaII CCGG 7 cut(s) 86, 101, 137, 859, 1230, 2471, 2645
HphI GGTGA 5 cut(s) 126, 202, 1464, 2033, 2105
Hpy166II GTNNAC 8 cut(s) 574, 595, 680, 955, 1133, 2457, 2530, 2656
Hpy8I GTNNAC 8 cut(s) 574, 595, 680, 955, 1133, 2457, 2530, 2656
HpyAV CCTTC 5 cut(s) 762, 1514, 2299, 2392, 2724
HpyCH4III ACNGT 5 cut(s) 282, 599, 1607, 2341, 2534
HpyCH4IV ACGT 5 cut(s) 203, 383, 2422, 2652, 2680
HpyF3I CTNAG 6 cut(s) 23, 330, 339, 1035, 1143, 1635
HpySE526I ACGT 5 cut(s) 203, 383, 2422, 2652, 2680
Hsp92I GRCGYC 5 cut(s) 128, 140, 203, 348, 2632
HspAI GCGC 4 cut(s) 300, 319, 1999, 2172
Kpn2I TCCGGA 1 cut(s) 2644
KpnI GGTACC 2 cut(s) 1043, 1939
Ksp22I TGATCA 1 cut(s) 1609
KspAI GTTAAC 1 cut(s) 595
KspI CCGCGG 1 cut(s) 75
Lsp1109I GCAGC 5 cut(s) 359, 631, 1175, 1212, 1897
LweI GCATC 9 cut(s) 405, 516, 656, 739, 1229, 1478, 2086, 2254, 2356
MabI ACCWGGT 1 cut(s) 1171
MaeI CTAG 4 cut(s) 290, 1047, 1716, 2220
MaeII ACGT 5 cut(s) 203, 383, 2422, 2652, 2680
MaeIII GTNAC 4 cut(s) 132, 2176, 2405, 2418
MbiI CCGCTC 3 cut(s) 225, 551, 1464
MfeI CAATTG 1 cut(s) 1853
MflI RGATCY 4 cut(s) 1090, 1123, 1750, 1981
MhlI GDGCHC 2 cut(s) 865, 1015
MlyI GAGTC 6 cut(s) 106, 194, 665, 1300, 2733, 2785
MmeI TCCRAC 7 cut(s) 724, 823, 1437, 2587, 2596, 2743, 2761
Mph1103I ATGCAT 1 cut(s) 2101
MroI TCCGGA 1 cut(s) 2644
MroXI GAANNNNTTC 2 cut(s) 1791, 1837
MslI CAYNNNNRTG 3 cut(s) 1100, 1548, 2151
MspA1I CMGCKG 1 cut(s) 74
MspCI CTTAAG 1 cut(s) 2357
MspI CCGG 7 cut(s) 86, 101, 137, 859, 1230, 2471, 2645
MspR9I CCNGG 4 cut(s) 102, 860, 1017, 1173
MunI CAATTG 1 cut(s) 1853
Mva1269I GAATGC 3 cut(s) 1786, 1912, 2286
MvaI CCWGG 2 cut(s) 1017, 1173
MvnI CGCG 5 cut(s) 74, 176, 319, 321, 353
NciI CCSGG 2 cut(s) 102, 860
NcoI CCATGG 2 cut(s) 1821, 2576
NdeI CATATG 3 cut(s) 640, 1225, 2017
NlaIV GGNNCC 7 cut(s) 47, 81, 437, 863, 1041, 1937, 2484
NmeAIII GCCGAG 2 cut(s) 348, 2695
NmuCI GTSAC 3 cut(s) 132, 2405, 2418
NsiI ATGCAT 1 cut(s) 2101
NspI RCATGY 1 cut(s) 1448
PagI TCATGA 3 cut(s) 1576, 1795, 1810
PcsI WCGNNNNNNNCGW 3 cut(s) 128, 137, 155
PctI GAATGC 3 cut(s) 1786, 1912, 2286
PdmI GAANNNNTTC 2 cut(s) 1791, 1837
PfeI GAWTC 8 cut(s) 112, 171, 314, 697, 1285, 1501, 1865, 2619
Pfl23II CGTACG 1 cut(s) 2423
PflFI GACNNNGTC 1 cut(s) 127
PflMI CCANNNNNTGG 2 cut(s) 55, 2731
PfoI TCCNGGA 1 cut(s) 1015
PkrI GCNGC 9 cut(s) 73, 178, 352, 374, 646, 1165, 1202, 1414, 1912
PleI GAGTC 6 cut(s) 105, 193, 664, 1300, 2733, 2785
PpsI GAGTC 6 cut(s) 105, 193, 664, 1300, 2733, 2785
Ppu21I YACGTR 2 cut(s) 2423, 2681
PsiI TTATAA 1 cut(s) 456
Psp6I CCWGG 2 cut(s) 1015, 1171
PspFI CCCAGC 2 cut(s) 1939, 2724
PspGI CCWGG 2 cut(s) 1015, 1171
PspLI CGTACG 1 cut(s) 2423
PspN4I GGNNCC 7 cut(s) 47, 81, 437, 863, 1041, 1937, 2484
PspOMI GGGCCC 1 cut(s) 861
PspPI GGNCC 9 cut(s) 46, 79, 286, 737, 861, 862, 1343, 1656, 2734
PstNI CAGNNNCTG 1 cut(s) 55
PsuI RGATCY 4 cut(s) 1090, 1123, 1750, 1981
PsyI GACNNNGTC 1 cut(s) 127
RsaI GTAC 6 cut(s) 279, 937, 1041, 1937, 2425, 2531
RsaNI GTAC 6 cut(s) 278, 936, 1040, 1936, 2424, 2530
RseI CAYNNNNRTG 3 cut(s) 1100, 1548, 2151
SacII CCGCGG 1 cut(s) 75
SalI GTCGAC 1 cut(s) 572
SatI GCNGC 9 cut(s) 72, 177, 351, 373, 645, 1164, 1201, 1413, 1911
Sau96I GGNCC 9 cut(s) 46, 79, 286, 737, 861, 862, 1343, 1656, 2734
SchI GAGTC 6 cut(s) 106, 194, 665, 1300, 2733, 2785
ScrFI CCNGG 4 cut(s) 102, 860, 1017, 1173
SduI GDGCHC 2 cut(s) 865, 1015
SexAI ACCWGGT 1 cut(s) 1171
SfaNI GCATC 9 cut(s) 405, 516, 656, 739, 1229, 1478, 2086, 2254, 2356
SfcI CTRYAG 2 cut(s) 1107, 2337
SfiI GGCCNNNNNGGCC 1 cut(s) 77
Sfr303I CCGCGG 1 cut(s) 75
SgrAI CRCCGGYG 2 cut(s) 85, 136
SgrBI CCGCGG 1 cut(s) 75
SinI GGWCC 4 cut(s) 46, 737, 1656, 2734
SmiMI CAYNNNNRTG 3 cut(s) 1100, 1548, 2151
SmlI CTYRAG 5 cut(s) 626, 1204, 1301, 2357, 2741
SmoI CTYRAG 5 cut(s) 626, 1204, 1301, 2357, 2741
SpeI ACTAGT 2 cut(s) 1046, 1715
SspMI CTAG 4 cut(s) 290, 1047, 1716, 2220
StyD4I CCNGG 4 cut(s) 100, 858, 1015, 1171
StyI CCWWGG 3 cut(s) 1821, 2219, 2576
TaaI ACNGT 5 cut(s) 282, 599, 1607, 2341, 2534
TaiI ACGT 5 cut(s) 206, 386, 2425, 2655, 2683
TaqI TCGA 8 cut(s) 5, 345, 503, 547, 573, 2143, 2350, 2794
TatI WGTACW 1 cut(s) 2529
TauI GCSGC 4 cut(s) 74, 179, 353, 1415
TfiI GAWTC 8 cut(s) 112, 171, 314, 697, 1285, 1501, 1865, 2619
TscAI CASTG 6 cut(s) 937, 1414, 1612, 2056, 2464, 2667
TseFI GTSAC 3 cut(s) 132, 2405, 2418
TseI GCWGC 5 cut(s) 372, 644, 1163, 1200, 1910
Tsp45I GTSAC 3 cut(s) 132, 2405, 2418
TspGWI ACGGA 2 cut(s) 373, 1657
TspRI CASTG 6 cut(s) 937, 1414, 1612, 2056, 2464, 2667
Tth111I GACNNNGTC 1 cut(s) 127
Van91I CCANNNNNTGG 2 cut(s) 55, 2731
Vha464I CTTAAG 1 cut(s) 2357
VpaK11BI GGWCC 4 cut(s) 46, 737, 1656, 2734
XceI RCATGY 1 cut(s) 1448
XmaJI CCTAGG 1 cut(s) 2219
XmiI GTMKAC 2 cut(s) 573, 679
XmnI GAANNNNTTC 2 cut(s) 1791, 1837
XspI CTAG 4 cut(s) 290, 1047, 1716, 2220
ZraI GACGTC 1 cut(s) 204
Zsp2I ATGCAT 1 cut(s) 2101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.