MD15G1060300.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006217
Physical Location & Seq
Forward (+)
4051894 .. 4058616
6723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1060300.v1.1.491

Sequence Viewer

Length: 2772 bp
ATGTCGAAGATGAAGCACCTACTGAGAAAGCTTCACATCGGCGGCGGACTCAATGAGCACCAGAGATTGGCGGAGGCCCGACCCTTGACGAGTCCGAGCGCGAATCTGAATCTGCCCGCTTCTTCTCCGGCGTCGTCCACGGGATCCTCTACAATGGGGAGAATCACCGCCGTTGAATCGGTGAGTGATCCGACGGCTGGTGGGGAGAGTGGTGGTAGCGGTAGCGGCGGGGGCGTGGGCGTGGATTTCAATTTCTTGGAGGAGGAGTTTCAGGTCCAGTTGGCCCTAGCGATCAGCGTTTCCGATCCCGATTCGCGCGACGACCCCGAGACGGCTCAGATCGACGCCGCCAAGCGGATTAGCCTCGGCTGCTCGGCGTCTAGTGTCACCGACACTCAAGCTCCCTTCGAGATGCTCTCGCTTCGCTACTGGAGCCAGAATGTTGTAGATTATAATGAAAAGGTGGTCGACGGATTTTATGACGTGTATGGAATGACCACAAATTTGCTTAGACAAGGGAAGATGCCATTGTTGGTGGATCTTAAAGCTGTATCTGTCTCGGAGAATGTTGATTATGACGTTATATTAGTGAACCGTTTGGTTGATCCTGATCTGCATCAACTTGAGAAAAGAGCATCTGCTGTCTTTTTAGAGTCACGGATTTCTCAACATGGTGTCCTTTTAAGTGGTTTGATTCAGAAAATTGCTGACATTGTTGTTGATAGAATGGGTGGTCCAGTTGCCGATGCTGATGAAATTTTGAGAAGGTGGAAAGTGAGGAGGTATGAGTTACGGAGTTCGATGAAAACTATCATTCTTCCCCTTGGACTTATTGATGTTGGACTTTCACGCCACAGGGCCCTGCTCTTTAAGGTACTGGCTGATAAGATAAACCTTCCATGTATGCTGGTCAAAGGTAGCTACTACACTGGTACTGATGATGGAGCTGTAAATTTGATTAAAATTGATAGTGGAAGTGAGTATATTATTGATTTGATGGGTGCACCTGGAACACTCATTCCTGCCGAGGTGCCTACTAGTCAGCTCCCGAATTCTTTCTTTGCCATAAGGAGCTTTCAAGATGCCACCGAAATGCCCAAAGATGTGTGTTTGGCTCAAGCTGAGGGCAGTGGAATGTTAGCAGTTCCTTCTGATCTTGACATAGTTTCTAGAACTGGCAGCTCACGGTCAGAAGAAGCATTGTATGCAGGCAGTCAAACAAAAGATGATAGAAGAATCGTTGTTGATGAAAACCAAATGGAGAGGTCTAGCAGTGATATGGGGACTGCCCTTAGATCACTTCGTAAATCATGTGAAAGTTCATCGGGTACAACTGAAAAAGCCACATCTGCGCAGAAAAGGAAAGTGAAAAATGTATCGAAGTATGTCATCAGTGCAGCAAAGAACCCAGAGTTTGCACAAAAACTACATGCTGTCTTGTTGGAAAGTGGTGCATCACCTCCTGCAGATTTATTTTCAGATATGAATCCTCAATATCTGCATGAAGACAAGTTGCTTGGTCAAATGAATGCAGATGGGAAACTTGTAGATGATGGGATTCATAATTATTTGGTCCAATTATTATCAGGCAATGAGCAGTCCACTCAAACTGCTGCTGAAGTGAGCTATGCCAATCTTGATAACTTGCTGAAACAATCTTCCTTGGATTTAGCTGAGCAACAAAATGAATCAGAGACAAATAAGTTTTCTCTTCTCTTAGATGCTGTTGAGGAGGGATTTGTAATCGTGTCTGGTGGAACTAGTGAAACAACCCAGATTTGTAATCCTGTTCTTGATAGTCCCCCAATGAACTCCAAAGCTTTTAACGAGTATAAAGAATCAGTGAGTAAACCAATGGACAGATGCAATAGTGGCCTGTGTACTAGTTGTGATAGTCACTACGAGAGGTATCCCGCACTGGGGGAAGTTGCAGAGTGGGAAATTCTATGGGAGGATCTTCAGATTGGTGAACGCATTGGCATTGGTTCCTATGGCGAGGTATATCGTGCAGATTGGAATGGCACTGAAGTAGCTGTGAAAAAGTTTTTAGATCAAGATTTTTCTGGTGATGCATTAGTTCAGTTTAAATGTGAAGTAGAAATTATGTTGAGACTCAGACATCCCAATGTTGTCCTTTTCATGGGGGCAGTTACTCGCCCTCCACATTTCTCTATCCTGACAGAGTTTCTTCCCAGGGGGAGTTTATATAGATTGCTGCATCGTCCCAATTCTCAACTTGATGAAAGGAGGCGAATGCGAATGGCTCTTGATGTGGCCAAGGGAATGAATTACCTGCACACTAGCAATCCTACTGTTGTGCATCGAGACCTAAAGTCTCCAAACCTCCTTGTTGATAAGAATTGGAATGTGAAGGTTTGTGACTTTGGGTTGTCACGCACGAAGCACCATACTTTTCTGTCTTCCAAGTCTACTGCTGGAACGCCCGAATGGATGGCTCCGGAAGTTTTAAGGAATGAACCGGCTAATGAGAAGTGTGATGTGTACAGCTTCGGTGTTATCTTATGGGAACTGGCTACTTGCTGCGTCCCATGGAAAGGATTGAACCCAATGCAGGTTGTTGGAGCCGTTGGATTCCAAAACAGGCGTCTTGAAATTCCAGAGGAAATCGATCCAGTGGTTGCCGAGATAATACGTGATTGTTGGCAAACAGAGCCAAACTTACGGCCATCTTTCTCGCAGCTCATGGTTCGCCTCCGACGCCTTCAACGTTTTGTTGGAAGAACAAACTCTACAAATCAAATGACTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

924

Amino Acids

101.92

Weight (kDa)

5.37

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 137 - 340 1.3e-73 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 653 - 904 1.2e-64 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 654 - 902 7.3e-52 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 453
Acc16I TGCGCA 1 cut(s) 1353
Acc36I ACCTGC 2 cut(s) 2301, 2563
AccB1I GGYRCC 1 cut(s) 1030
AccB7I CCANNNNNTGG 1 cut(s) 67
AccI GTMKAC 2 cut(s) 468, 2429
AccII CGCG 3 cut(s) 101, 316, 318
AccIII TCCGGA 1 cut(s) 2458
AclI AACGTT 1 cut(s) 2728
AclWI GGATC 8 cut(s) 138, 151, 182, 299, 546, 599, 1962, 2624
AcoI YGGCCR 2 cut(s) 2274, 2684
AcsI RAATTY 6 cut(s) 502, 756, 952, 1051, 1941, 2613
AcuI CTGAAG 3 cut(s) 1638, 1943, 2046
AcyI GRCGYC 5 cut(s) 131, 345, 377, 2605, 2719
AfaI GTAC 5 cut(s) 876, 934, 1330, 1882, 2504
AfiI CCNNNNNNNGG 9 cut(s) 67, 197, 331, 354, 1919, 1920, 2140, 2555, 2572
AflIII ACRYGT 1 cut(s) 483
AgsI TTSAA 6 cut(s) 176, 250, 1079, 2563, 2612, 2726
AhdI GACNNNNNGTC 1 cut(s) 2332
AhlI ACTAGT 3 cut(s) 1037, 1760, 1883
AjiI CACGTC 1 cut(s) 484
AjnI CCWGG 2 cut(s) 1006, 2192
AloI GAACNNNNNNTCC 2 cut(s) 1003, 1035
Alw21I GWGCWC 2 cut(s) 60, 1006
Alw26I GTCTC 6 cut(s) 323, 562, 1688, 2104, 2319, 2340
Alw44I GTGCAC 1 cut(s) 1002
AlwI GGATC 8 cut(s) 138, 151, 182, 299, 546, 599, 1962, 2624
Ama87I CYCGRG 1 cut(s) 326
Aor13HI TCCGGA 1 cut(s) 2458
AoxI GGCC 6 cut(s) 75, 282, 858, 1873, 2274, 2684
ApaI GGGCCC 1 cut(s) 862
ApaLI GTGCAC 1 cut(s) 1002
ApeKI GCWGC 7 cut(s) 369, 1179, 1397, 1613, 2215, 2541, 2698
ApoI RAATTY 6 cut(s) 502, 756, 952, 1051, 1941, 2613
ArsI GACNNNNNNTTYG 4 cut(s) 487, 519, 2590, 2622
Asp700I GAANNNNTTC 1 cut(s) 1055
AspLEI GCGC 3 cut(s) 101, 318, 1354
AspS9I GGNCC 7 cut(s) 76, 274, 283, 734, 858, 859, 1573
AsuHPI GGTGA 6 cut(s) 157, 193, 379, 1449, 1979, 2078
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 3 cut(s) 274, 734, 1573
BaeGI GKGCMC 2 cut(s) 862, 1006
BalI TGGCCA 1 cut(s) 2276
BamHI GGATCC 1 cut(s) 143
BanI GGYRCC 1 cut(s) 1030
BanII GRGCYC 1 cut(s) 862
BbsI GAAGAC 2 cut(s) 1512, 2412
Bbv12I GWGCWC 2 cut(s) 60, 1006
BbvCI CCTCAGC 1 cut(s) 1122
BbvI GCAGC 7 cut(s) 356, 1191, 1409, 1600, 2202, 2528, 2710
BccI CCATC 6 cut(s) 935, 991, 1529, 1547, 2446, 2695
BceAI ACGGC 5 cut(s) 155, 210, 348, 2570, 2699
BciT130I CCWGG 2 cut(s) 1008, 2194
BciVI GTATCC 1 cut(s) 1920
BcoDI GTCTC 6 cut(s) 323, 562, 1688, 2104, 2319, 2340
BcuI ACTAGT 3 cut(s) 1037, 1760, 1883
BfaI CTAG 8 cut(s) 287, 381, 1038, 1170, 1269, 1761, 1884, 2301
BfmI CTRYAG 1 cut(s) 1464
BfuAI ACCTGC 2 cut(s) 2301, 2563
BfuI GTATCC 1 cut(s) 1920
BlpI GCTNAGC 1 cut(s) 1674
Bme1390I CCNGG 2 cut(s) 1008, 2194
Bme18I GGWCC 3 cut(s) 274, 734, 1573
BmeRI GACNNNNNGTC 1 cut(s) 2332
BmeT110I CYCGRG 1 cut(s) 326
BmgBI CACGTC 1 cut(s) 484
BmgT120I GGNCC 7 cut(s) 76, 274, 283, 734, 858, 859, 1573
BmiI GGNNCC 7 cut(s) 145, 434, 860, 1032, 1987, 2457, 2584
BmrFI CCNGG 2 cut(s) 1008, 2194
BmrI ACTGGG 1 cut(s) 1928
BmuI ACTGGG 1 cut(s) 1928
BpiI GAAGAC 2 cut(s) 1512, 2412
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpmI CTGGAG 1 cut(s) 451
Bpu10I CCTNAGC 1 cut(s) 1122
Bpu1102I GCTNAGC 1 cut(s) 1674
BpuEI CTTGAG 3 cut(s) 381, 644, 1101
Bsa29I ATCGAT 1 cut(s) 2628
BsaAI YACGTR 1 cut(s) 2654
BsaBI GATNNNNATC 3 cut(s) 609, 615, 1485
BsaHI GRCGYC 5 cut(s) 131, 345, 377, 2605, 2719
BsaI GGTCTC 1 cut(s) 2319
BsaJI CCNNGG 9 cut(s) 138, 364, 823, 1026, 1662, 2192, 2193, 2277, 2549
BsaWI WCCGGW 1 cut(s) 2458
BsaXI ACNNNNNCTCC 6 cut(s) 251, 281, 385, 415, 2143, 2173
Bsc4I CCNNNNNNNGG 9 cut(s) 67, 197, 331, 354, 1919, 1920, 2140, 2555, 2572
Bse118I RCCGGY 1 cut(s) 2479
Bse1I ACTGG 9 cut(s) 277, 434, 737, 882, 934, 1180, 1923, 2535, 2633
Bse3DI GCAATG 1 cut(s) 1597
Bse8I GATNNNNATC 3 cut(s) 609, 615, 1485
BseAI TCCGGA 1 cut(s) 2458
BseBI CCWGG 2 cut(s) 1008, 2194
BseCI ATCGAT 1 cut(s) 2628
BseDI CCNNGG 9 cut(s) 138, 364, 823, 1026, 1662, 2192, 2193, 2277, 2549
BseGI GGATG 2 cut(s) 2119, 2457
BseJI GATNNNNATC 3 cut(s) 609, 615, 1485
BseLI CCNNNNNNNGG 9 cut(s) 67, 197, 331, 354, 1919, 1920, 2140, 2555, 2572
BseMI GCAATG 1 cut(s) 1597
BseMII CTCAG 5 cut(s) 14, 350, 1113, 1665, 2128
BseNI ACTGG 9 cut(s) 277, 434, 737, 882, 934, 1180, 1923, 2535, 2633
BseRI GAGGAG 4 cut(s) 275, 278, 793, 1745
BseSI GKGCMC 2 cut(s) 862, 1006
BseXI GCAGC 7 cut(s) 356, 1191, 1409, 1600, 2202, 2528, 2710
BsgI GTGCAG 3 cut(s) 1416, 2028, 2279
Bsh1236I CGCG 3 cut(s) 101, 316, 318
BshFI GGCC 6 cut(s) 77, 284, 860, 1875, 2276, 2686
BshNI GGYRCC 1 cut(s) 1030
BshVI ATCGAT 1 cut(s) 2628
BsiHKAI GWGCWC 2 cut(s) 60, 1006
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 3 cut(s) 128, 2459, 2480
BslFI GGGAC 4 cut(s) 1297, 1785, 2208, 2531
BslI CCNNNNNNNGG 9 cut(s) 67, 197, 331, 354, 1919, 1920, 2140, 2555, 2572
BsmAI GTCTC 6 cut(s) 323, 562, 1688, 2104, 2319, 2340
BsmBI CGTCTC 1 cut(s) 323
BsmFI GGGAC 4 cut(s) 1297, 1785, 2208, 2531
BsmI GAATGC 2 cut(s) 1534, 2259
BsnI GGCC 6 cut(s) 77, 284, 860, 1875, 2276, 2686
Bso31I GGTCTC 1 cut(s) 2319
BsoBI CYCGRG 1 cut(s) 326
Bsp120I GGGCCC 1 cut(s) 858
Bsp1286I GDGCHC 3 cut(s) 60, 862, 1006
Bsp13I TCCGGA 1 cut(s) 2458
Bsp1407I TGTACA 1 cut(s) 2502
Bsp1720I GCTNAGC 1 cut(s) 1674
Bsp19I CCATGG 1 cut(s) 2549
BspANI GGCC 6 cut(s) 77, 284, 860, 1875, 2276, 2686
BspCNI CTCAG 5 cut(s) 15, 349, 1114, 1666, 2127
BspDI ATCGAT 1 cut(s) 2628
BspEI TCCGGA 1 cut(s) 2458
BspFNI CGCG 3 cut(s) 101, 316, 318
BspLI GGNNCC 7 cut(s) 145, 434, 860, 1032, 1987, 2457, 2584
BspMAI CTGCAG 1 cut(s) 1468
BspMI ACCTGC 2 cut(s) 2301, 2563
BspPI GGATC 8 cut(s) 138, 151, 182, 299, 546, 599, 1962, 2624
BspT107I GGYRCC 1 cut(s) 1030
BspTNI GGTCTC 1 cut(s) 2319
BsrDI GCAATG 1 cut(s) 1597
BsrFI RCCGGY 1 cut(s) 2479
BsrGI TGTACA 1 cut(s) 2502
BsrI ACTGG 9 cut(s) 277, 434, 737, 882, 934, 1180, 1923, 2535, 2633
BssAI RCCGGY 1 cut(s) 2479
BssECI CCNNGG 9 cut(s) 138, 364, 823, 1026, 1662, 2192, 2193, 2277, 2549
BssNI GRCGYC 5 cut(s) 131, 345, 377, 2605, 2719
BssT1I CCWWGG 4 cut(s) 823, 1662, 2277, 2549
Bst2UI CCWGG 2 cut(s) 1008, 2194
Bst4CI ACNGT 3 cut(s) 596, 1188, 2314
Bst6I CTCTTC 1 cut(s) 1716
BstACI GRCGYC 5 cut(s) 131, 345, 377, 2605, 2719
BstAPI GCANNNNNTGC 1 cut(s) 1205
BstAUI TGTACA 1 cut(s) 2502
BstBAI YACGTR 1 cut(s) 2654
BstC8I GCNNGC 2 cut(s) 117, 1210
BstDEI CTNAG 8 cut(s) 23, 336, 509, 1122, 1292, 1674, 1717, 2114
BstDSI CCRYGG 2 cut(s) 138, 2549
BstF5I GGATG 2 cut(s) 2119, 2457
BstFNI CGCG 3 cut(s) 101, 316, 318
BstHHI GCGC 3 cut(s) 101, 318, 1354
BstMAI GTCTC 6 cut(s) 323, 562, 1688, 2104, 2319, 2340
BstMWI GCNNNNNNNGC 9 cut(s) 225, 231, 369, 432, 1205, 1349, 1872, 2671, 2718
BstNI CCWGG 2 cut(s) 1008, 2194
BstNSI RCATGY 1 cut(s) 1433
BstSCI CCNGG 2 cut(s) 1006, 2192
BstSFI CTRYAG 1 cut(s) 1464
BstSLI GKGCMC 2 cut(s) 862, 1006
BstUI CGCG 3 cut(s) 101, 316, 318
BstV1I GCAGC 7 cut(s) 356, 1191, 1409, 1600, 2202, 2528, 2710
BstV2I GAAGAC 2 cut(s) 1512, 2412
BstX2I RGATCY 3 cut(s) 143, 538, 1954
BstYI RGATCY 3 cut(s) 143, 538, 1954
Bsu15I ATCGAT 1 cut(s) 2628
BsuI GTATCC 1 cut(s) 1920
BsuRI GGCC 6 cut(s) 77, 284, 860, 1875, 2276, 2686
BsuTUI ATCGAT 1 cut(s) 2628
BtgI CCRYGG 2 cut(s) 138, 2549
BtrI CACGTC 1 cut(s) 484
BtsCI GGATG 2 cut(s) 2119, 2457
BtsI GCAGTG 2 cut(s) 1135, 1279
BtsIMutI CAGTG 8 cut(s) 927, 1135, 1279, 1399, 1848, 1916, 2022, 2640
BveI ACCTGC 2 cut(s) 2301, 2563
Cac8I GCNNGC 2 cut(s) 117, 1210
CfoI GCGC 3 cut(s) 101, 318, 1354
Cfr10I RCCGGY 1 cut(s) 2479
Cfr13I GGNCC 7 cut(s) 76, 274, 283, 734, 858, 859, 1573
ClaI ATCGAT 1 cut(s) 2628
CseI GACGC 6 cut(s) 120, 353, 366, 2533, 2594, 2727
Csp6I GTAC 5 cut(s) 875, 933, 1329, 1881, 2503
CviAII CATG 8 cut(s) 671, 900, 1311, 1430, 1502, 2140, 2550, 2704
CviQI GTAC 5 cut(s) 875, 933, 1329, 1881, 2503
DdeI CTNAG 8 cut(s) 23, 336, 509, 1122, 1292, 1674, 1717, 2114
DraI TTTAAA 1 cut(s) 2086
DriI GACNNNNNGTC 1 cut(s) 2332
EaeI YGGCCR 2 cut(s) 2274, 2684
Eam1104I CTCTTC 1 cut(s) 1716
Eam1105I GACNNNNNGTC 1 cut(s) 2332
EarI CTCTTC 1 cut(s) 1716
EciI GGCGGA 2 cut(s) 60, 86
Eco130I CCWWGG 4 cut(s) 823, 1662, 2277, 2549
Eco24I GRGCYC 1 cut(s) 862
Eco31I GGTCTC 1 cut(s) 2319
Eco47I GGWCC 3 cut(s) 274, 734, 1573
Eco57I CTGAAG 3 cut(s) 1638, 1943, 2046
Eco88I CYCGRG 1 cut(s) 326
EcoO109I RGGNCCY 2 cut(s) 858, 859
EcoRI GAATTC 1 cut(s) 1051
EcoRII CCWGG 2 cut(s) 1006, 2192
EcoT14I CCWWGG 4 cut(s) 823, 1662, 2277, 2549
EcoT22I ATGCAT 1 cut(s) 2074
EcoT38I GRGCYC 1 cut(s) 862
ErhI CCWWGG 4 cut(s) 823, 1662, 2277, 2549
Esp3I CGTCTC 1 cut(s) 323
FaeI CATG 8 cut(s) 674, 903, 1314, 1433, 1505, 2143, 2553, 2707
FaqI GGGAC 4 cut(s) 1297, 1785, 2208, 2531
FatI CATG 8 cut(s) 670, 899, 1310, 1429, 1501, 2139, 2549, 2703
FauI CCCGC 3 cut(s) 124, 221, 1921
FblI GTMKAC 2 cut(s) 468, 2429
FokI GGATG 2 cut(s) 2106, 2464
FriOI GRGCYC 1 cut(s) 862
FspBI CTAG 8 cut(s) 287, 381, 1038, 1170, 1269, 1761, 1884, 2301
FspI TGCGCA 1 cut(s) 1353
GlaI GCGC 3 cut(s) 100, 317, 1353
GsuI CTGGAG 1 cut(s) 451
HaeIII GGCC 6 cut(s) 77, 284, 860, 1875, 2276, 2686
HapII CCGG 3 cut(s) 128, 2459, 2480
HgaI GACGC 6 cut(s) 120, 353, 366, 2533, 2594, 2727
HhaI GCGC 3 cut(s) 101, 318, 1354
Hin1I GRCGYC 5 cut(s) 131, 345, 377, 2605, 2719
Hin1II CATG 8 cut(s) 674, 903, 1314, 1433, 1505, 2143, 2553, 2707
Hin6I GCGC 3 cut(s) 99, 316, 1352
HinP1I GCGC 3 cut(s) 99, 316, 1352
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 2 cut(s) 29, 1818
HpaII CCGG 3 cut(s) 128, 2459, 2480
HphI GGTGA 6 cut(s) 157, 193, 379, 1449, 1979, 2078
Hpy99I CGWCG 6 cut(s) 136, 196, 323, 347, 473, 2721
HpyAV CCTTC 6 cut(s) 415, 759, 905, 1158, 2365, 2732
HpyCH4III ACNGT 3 cut(s) 596, 1188, 2314
HpyCH4IV ACGT 4 cut(s) 483, 579, 2653, 2728
HpyF10VI GCNNNNNNNGC 9 cut(s) 225, 231, 369, 432, 1205, 1349, 1872, 2671, 2718
HpyF3I CTNAG 8 cut(s) 23, 336, 509, 1122, 1292, 1674, 1717, 2114
HpySE526I ACGT 4 cut(s) 483, 579, 2653, 2728
Hsp92I GRCGYC 5 cut(s) 131, 345, 377, 2605, 2719
Hsp92II CATG 8 cut(s) 674, 903, 1314, 1433, 1505, 2143, 2553, 2707
HspAI GCGC 3 cut(s) 99, 316, 1352
Kpn2I TCCGGA 1 cut(s) 2458
LmnI GCTCC 7 cut(s) 406, 432, 944, 1050, 1071, 2461, 2582
Lsp1109I GCAGC 7 cut(s) 356, 1191, 1409, 1600, 2202, 2528, 2710
MaeI CTAG 8 cut(s) 287, 381, 1038, 1170, 1269, 1761, 1884, 2301
MaeII ACGT 4 cut(s) 483, 579, 2653, 2728
MaeIII GTNAC 7 cut(s) 385, 654, 789, 1895, 2149, 2378, 2391
MflI RGATCY 3 cut(s) 143, 538, 1954
MhlI GDGCHC 3 cut(s) 60, 862, 1006
MlsI TGGCCA 1 cut(s) 2276
MluNI TGGCCA 1 cut(s) 2276
MlyI GAGTC 4 cut(s) 42, 100, 662, 2106
MmeI TCCRAC 7 cut(s) 215, 820, 1422, 2560, 2569, 2716, 2740
Mox20I TGGCCA 1 cut(s) 2276
Mph1103I ATGCAT 1 cut(s) 2074
MroI TCCGGA 1 cut(s) 2458
MroXI GAANNNNTTC 1 cut(s) 1055
MscI TGGCCA 1 cut(s) 2276
MseI TTAA 7 cut(s) 543, 683, 870, 960, 1824, 2085, 2468
MslI CAYNNNNRTG 2 cut(s) 1091, 2124
Msp20I TGGCCA 1 cut(s) 2276
MspI CCGG 3 cut(s) 128, 2459, 2480
MspR9I CCNGG 2 cut(s) 1008, 2194
Mva1269I GAATGC 2 cut(s) 1534, 2259
MvaI CCWGG 2 cut(s) 1008, 2194
MvnI CGCG 3 cut(s) 101, 316, 318
MwoI GCNNNNNNNGC 9 cut(s) 225, 231, 369, 432, 1205, 1349, 1872, 2671, 2718
NcoI CCATGG 1 cut(s) 2549
NlaIII CATG 8 cut(s) 674, 903, 1314, 1433, 1505, 2143, 2553, 2707
NlaIV GGNNCC 7 cut(s) 145, 434, 860, 1032, 1987, 2457, 2584
NmeAIII GCCGAG 4 cut(s) 345, 353, 1051, 2668
NmuCI GTSAC 5 cut(s) 385, 654, 1895, 2378, 2391
NsbI TGCGCA 1 cut(s) 1353
NsiI ATGCAT 1 cut(s) 2074
NspI RCATGY 1 cut(s) 1433
PasI CCCWGGG 1 cut(s) 2193
PcsI WCGNNNNNNNCGW 1 cut(s) 2725
PctI GAATGC 2 cut(s) 1534, 2259
PdmI GAANNNNTTC 1 cut(s) 1055
PflMI CCANNNNNTGG 1 cut(s) 67
PleI GAGTC 4 cut(s) 42, 99, 661, 2106
PpsI GAGTC 4 cut(s) 42, 99, 661, 2106
Ppu21I YACGTR 1 cut(s) 2654
PsiI TTATAA 1 cut(s) 453
Psp1406I AACGTT 1 cut(s) 2728
Psp6I CCWGG 2 cut(s) 1006, 2192
PspGI CCWGG 2 cut(s) 1006, 2192
PspN4I GGNNCC 7 cut(s) 145, 434, 860, 1032, 1987, 2457, 2584
PspOMI GGGCCC 1 cut(s) 858
PspPI GGNCC 7 cut(s) 76, 274, 283, 734, 858, 859, 1573
PsrI GAACNNNNNNTAC 2 cut(s) 2734, 2766
PstI CTGCAG 1 cut(s) 1468
PsuI RGATCY 3 cut(s) 143, 538, 1954
RsaI GTAC 5 cut(s) 876, 934, 1330, 1882, 2504
RsaNI GTAC 5 cut(s) 875, 933, 1329, 1881, 2503
RseI CAYNNNNRTG 2 cut(s) 1091, 2124
SalI GTCGAC 1 cut(s) 467
SaqAI TTAA 7 cut(s) 543, 683, 870, 960, 1824, 2085, 2468
Sau96I GGNCC 7 cut(s) 76, 274, 283, 734, 858, 859, 1573
SchI GAGTC 4 cut(s) 42, 100, 662, 2106
ScrFI CCNGG 2 cut(s) 1008, 2194
SduI GDGCHC 3 cut(s) 60, 862, 1006
SfcI CTRYAG 1 cut(s) 1464
SinI GGWCC 3 cut(s) 274, 734, 1573
SmiMI CAYNNNNRTG 2 cut(s) 1091, 2124
SmlI CTYRAG 3 cut(s) 396, 623, 1116
SmoI CTYRAG 3 cut(s) 396, 623, 1116
SpeI ACTAGT 3 cut(s) 1037, 1760, 1883
SspMI CTAG 8 cut(s) 287, 381, 1038, 1170, 1269, 1761, 1884, 2301
StyD4I CCNGG 2 cut(s) 1006, 2192
StyI CCWWGG 4 cut(s) 823, 1662, 2277, 2549
TaaI ACNGT 3 cut(s) 596, 1188, 2314
TaiI ACGT 4 cut(s) 486, 582, 2656, 2731
TaqI TCGA 8 cut(s) 5, 342, 408, 468, 800, 1379, 2323, 2628
TatI WGTACW 2 cut(s) 1880, 2502
TauI GCSGC 3 cut(s) 45, 228, 350
Tru1I TTAA 7 cut(s) 543, 683, 870, 960, 1824, 2085, 2468
Tru9I TTAA 7 cut(s) 543, 683, 870, 960, 1824, 2085, 2468
TscAI CASTG 8 cut(s) 934, 1135, 1279, 1399, 1848, 1923, 2029, 2640
TseFI GTSAC 5 cut(s) 385, 654, 1895, 2378, 2391
TseI GCWGC 7 cut(s) 369, 1179, 1397, 1613, 2215, 2541, 2698
Tsp45I GTSAC 5 cut(s) 385, 654, 1895, 2378, 2391
TspGWI ACGGA 3 cut(s) 486, 673, 808
TspRI CASTG 8 cut(s) 934, 1135, 1279, 1399, 1848, 1923, 2029, 2640
Van91I CCANNNNNTGG 1 cut(s) 67
VneI GTGCAC 1 cut(s) 1002
VpaK11BI GGWCC 3 cut(s) 274, 734, 1573
XapI RAATTY 6 cut(s) 502, 756, 952, 1051, 1941, 2613
XbaI TCTAGA 1 cut(s) 1169
XceI RCATGY 1 cut(s) 1433
XmiI GTMKAC 2 cut(s) 468, 2429
XmnI GAANNNNTTC 1 cut(s) 1055
XspI CTAG 8 cut(s) 287, 381, 1038, 1170, 1269, 1761, 1884, 2301
Zsp2I ATGCAT 1 cut(s) 2074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.