Prupe.1G412200_v2.0.a1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0006217
Physical Location & Seq
Forward (+)
35969091 .. 35975101
6011 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G412200.1

Sequence Viewer

Length: 2772 bp
ATGTCGAAGATGAAGCACCTACTAAGAAAGCTTCACATCGGTGGAGGACTCAATGAGCACCAAAGACTGGCCGAGACCCGACCCGAGACGAGTCCGAGCACGAATCTCAACCCTACCGCTTCTTCGCCGGCGTCGTCCACGGGTTCGGCTACAATGGGGAGAATCACGGCCGTTGAATCGGTGAGTGATCGGACGGCTGGGGACGGTGGTAGCGGTGGGGGCGTGGATTACAATTTATTGGAGGAGGAGTTTCAGGTCCAGTTGGCCTTAGCGATCAGCGCTTCGGATCCCGATTCGCGCGACGACCCCGATTCGGCTCAGATCGACGCCGCCAAGCGGATTAGCCTCGGCTGCCCGGCTACCGTCACCGACACTCAAGCCCCCTTCGAGATTCTCTCACTTCGCTATTGGAGCCAGAATGTTGTAGATTACAATGAAAAAGTGGTCGATGGATTTTATGACGTGTATGGAATGACCTCAAATTCTCTTAGACAAGGGAAGATGCCATTGTTAGTGGATCTTCAAGCTGTGTCTGTTTCAGATAATGTTGATTATGATGTCATATTAGTTAACCGGTTGGTGGATCCTGAACTGCAACAACTGGAGAAAACAGCATACGCTGTATCTCTAGAGTCACGGATTTCTCAACATGGTGTCCTTTTAAGTGGATTAATTCAGAAAATTGCTGATATTGTTGTTGATAGAATGGGTGGTCCAGTTGGGGATGCTGATGAAATTTTGAGAAGGTGGAAAGTGAGGAGGTACGAGTTACGGAGTTCGATGAAGACTATCATTCTTCCCCTTGGACTTATTGATGTTGGACTTTCACGCCACAGGGCGCTGCTCTTTAAGGTACTGGCTGATAGGATAAATCTTCCATGTATGCTGGTCAAAGGTAGCTACTACACTGGTACTGATGATGGAGCTGTAAACTTGATTAAAATTGATAGTGGAAGTGAATATATTATTGATCTGATGGGTGCTCCTGGAACGCTTATTCCTGCCGAGGTGCCCAGTAGCCAGCTACCAAATTCTTTCTTTGCCATAAGGAGCTTTCAAGATGCCACCGAATTGCCCAAAGATATGTGTTTGTTGCAAGCTGAAGGCACTGGAATGTTGGCAGTTCCACCCGATCTTGACAGACTTTCTAGAGTTGGCAGCTCACAGTCAGAAGAAGCATCATATGTAGGTGTTCAAACAAAAAATGATAGAAGCGTTGTTGAAGAAAATCAAACTGAGAGTTTGAGAAGCGAGATTGGGACTCCTCTTAGATCACTTCGTAAATCATGTGAAAGTTCATCAGGCACATCTGAGAAAGCCACATCTGCACAAAAAAGGAAAGTGAAAAATGTGTCAAAGTATGTCATCAGTGCAGCAAAGAACCCAGAATTTGCACAGAAACTACATGCTGTATTGTTGGAGAGTGGTGCATCACCTCCCCCAGATTTATTTTCAGATATGAATCCTCAATATCTGGACGAAGCTAAATTGCTTGATCAAATCCATGCAAATGGGAAACTTGTAGATGATGGGATTCATAATTATCTGGTTCAGTTATTATCAGGCAACGAGCAATCCACTCAAGCTGCTGCTGCAGTGAGCTATGACAATTTTGATAACTTTCTAAAACAATCTGCCGTGGACTTAGCAGAGCAACGAAATGAATTGGAGACGAACATTTTATCTCTTCCCTCTGATACTGTTGATGAGGGATTTGTAATTGTTTCTGGTGGAACTAGTGAAACAACCCAGATCGGTGCTAAAAGTTCAGATCCTGTTCTTGTTAGTCCCCAAGGAATGAATTCAGAAGCTTTTCATGAGGATAAAAGCCATGAACTTTCCTTATCTAAACCAATGGAAACAGCCAATAGTGGCCTGTGTACTAGTTGCGATAGTCACTATGAGAGGTACCCAGCACTGGGAGAAGTTGCAGAGTGGGAAATTTTATGGGAGGATCTTCAGATTGGTGAGCGCATTGGCATTGGTTCATATGGCGAGGTATATCATGCAGATTGGAATGGCACTGAAGTAGCTGTGAAAAAATTTTTGGATCAAGATTTTTCTGGTGATGCTTTAGTTCAGTTTAAATGTGAAGTAGAAATTATGTTAAGACTTAGACATCCCAATGTTGTCCTTTTCATGGGGGCAGTTACTCGCCCTCCCCATTTCTCTATCCTGACAGAGTATCTTCCCAGGGGGAGTTTATATAGACTTTTGCATCGTCCCAATTCTCAACTTGATGAAAAGAGGCGAATGCGAATGGCTTTTGATGTGGCCAAAGGAATGAATTACCTGCATACTAGCCATCCTACTGTTGTGCATCGAGACCTGAAGTCTCCAAATCTCCTTGTTGATAAGAATTGGAATGTGAAGGTTTGTGACTTTGGGTTGTCACGTACAAAGCACCATACTTTTCTGTCTTCGAAGTCTACTGCTGGGACGCCCGAGTGGATGGCACCGGAAGTTTTAAGGAATGAACCAGCTAATGAGAAGTGTGATGTGTACAGTTTTGGTGTCATCTTATGGGAACTGGCTACTTGCTGCGTCCCATGGAAAGGATTGAATCCAATGCAGGTTGTAGGAGCGGTTGGATTCCAAAATAGGCGTCTTGAAATTCCAGAGGACATGGATCCAGTGGTTGCTGAGATAATACGTGATTGTTGGCAAAGAGAGCCAAATCTACGGCCTTCGTTCTCGCAACTCATGGTCCGTCTCCGACGTCTTCAACGCCTTGTTGGAAGAACAAACTCTACAAACCAAACGACTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

924

Amino Acids

101.95

Weight (kDa)

5.28

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2722
Acc36I ACCTGC 2 cut(s) 2301, 2563
Acc65I GGTACC 1 cut(s) 1908
AccB1I GGYRCC 3 cut(s) 1009, 1908, 2455
AccB7I CCANNNNNTGG 1 cut(s) 67
AccBSI CCGCTC 1 cut(s) 2585
AccI GTMKAC 1 cut(s) 2429
AccII CGCG 2 cut(s) 298, 300
AciI CCGC 5 cut(s) 117, 213, 330, 337, 2585
AcoI YGGCCR 3 cut(s) 69, 168, 2274
AcsI RAATTY 8 cut(s) 481, 735, 1030, 1388, 1801, 1941, 2042, 2613
AcuI CTGAAG 4 cut(s) 1122, 1943, 2046, 2351
AcyI GRCGYC 5 cut(s) 131, 327, 2441, 2605, 2719
AfaI GTAC 7 cut(s) 764, 855, 913, 1882, 1910, 2398, 2504
AfeI AGCGCT 1 cut(s) 280
AfiI CCNNNNNNNGG 8 cut(s) 67, 313, 336, 580, 1918, 1919, 2140, 2555
AflIII ACRYGT 1 cut(s) 462
AgeI ACCGGT 1 cut(s) 573
AgsI TTSAA 8 cut(s) 176, 524, 1058, 1196, 1223, 2563, 2612, 2726
AhdI GACNNNNNGTC 1 cut(s) 2332
AhlI ACTAGT 2 cut(s) 1736, 1883
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 2 cut(s) 985, 2192
AjuI GAANNNNNNNTTGG 2 cut(s) 2030, 2062
AleI CACNNNNGTG 1 cut(s) 39
AloI GAACNNNNNNTCC 2 cut(s) 982, 1014
Alw21I GWGCWC 3 cut(s) 60, 101, 985
Alw26I GTCTC 6 cut(s) 68, 80, 1664, 2319, 2340, 2717
AlwNI CAGNNNCTG 1 cut(s) 1775
Ama87I CYCGRG 2 cut(s) 83, 2444
Aor51HI AGCGCT 1 cut(s) 280
AoxI GGCC 6 cut(s) 69, 168, 264, 1873, 2274, 2684
ApeKI GCWGC 8 cut(s) 351, 841, 1158, 1373, 1586, 1589, 1592, 2541
ApoI RAATTY 8 cut(s) 481, 735, 1030, 1388, 1801, 1941, 2042, 2613
ArsI GACNNNNNNTTYG 2 cut(s) 2590, 2622
AseI ATTAAT 1 cut(s) 671
AsiGI ACCGGT 1 cut(s) 573
Asp700I GAANNNNTTC 2 cut(s) 1801, 1812
Asp718I GGTACC 1 cut(s) 1908
AspLEI GCGC 4 cut(s) 281, 300, 841, 1974
AspS9I GGNCC 3 cut(s) 256, 713, 2707
AsuC2I CCSGG 1 cut(s) 356
AsuHPI GGTGA 5 cut(s) 193, 358, 1425, 1979, 2078
AsuII TTCGAA 1 cut(s) 2423
AvaI CYCGRG 2 cut(s) 83, 2444
AvaII GGWCC 3 cut(s) 256, 713, 2707
BaeGI GKGCMC 1 cut(s) 1014
BalI TGGCCA 1 cut(s) 2276
BamHI GGATCC 3 cut(s) 286, 583, 2629
BanI GGYRCC 3 cut(s) 1009, 1908, 2455
BbsI GAAGAC 3 cut(s) 791, 2412, 2714
Bbv12I GWGCWC 3 cut(s) 60, 101, 985
BbvI GCAGC 8 cut(s) 338, 828, 1170, 1385, 1573, 1576, 1579, 2528
BccI CCATC 6 cut(s) 443, 914, 970, 1523, 2313, 2446
BceAI ACGGC 5 cut(s) 155, 183, 210, 1622, 2699
BciT130I CCWGG 2 cut(s) 987, 2194
BclI TGATCA 1 cut(s) 1495
BcnI CCSGG 1 cut(s) 356
BcoDI GTCTC 6 cut(s) 68, 80, 1664, 2319, 2340, 2717
BcuI ACTAGT 2 cut(s) 1736, 1883
BfaI CTAG 5 cut(s) 629, 1149, 1737, 1884, 2301
BfmI CTRYAG 1 cut(s) 1593
BfoI RGCGCY 2 cut(s) 282, 842
BfuAI ACCTGC 2 cut(s) 2301, 2563
BisI GCNGC 9 cut(s) 330, 352, 842, 1159, 1374, 1587, 1590, 1593, 2542
BlsI GCNGC 9 cut(s) 331, 353, 843, 1160, 1375, 1588, 1591, 1594, 2543
Bme1390I CCNGG 3 cut(s) 356, 987, 2194
Bme18I GGWCC 3 cut(s) 256, 713, 2707
BmeRI GACNNNNNGTC 1 cut(s) 2332
BmeT110I CYCGRG 2 cut(s) 83, 2444
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 3 cut(s) 256, 713, 2707
BmiI GGNNCC 7 cut(s) 288, 413, 585, 1011, 1910, 2457, 2631
BmrFI CCNGG 3 cut(s) 356, 987, 2194
BmrI ACTGGG 2 cut(s) 1008, 1928
BmsI GCATC 8 cut(s) 492, 715, 1051, 1187, 1439, 2059, 2227, 2329
BmuI ACTGGG 2 cut(s) 1008, 1928
BpiI GAAGAC 3 cut(s) 791, 2412, 2714
BplI GAGNNNNNCTC 2 cut(s) 380, 412
BpmI CTGGAG 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 268
Bpu14I TTCGAA 1 cut(s) 2423
BpuEI CTTGAG 2 cut(s) 360, 1566
BpuMI CCSGG 1 cut(s) 356
BsaAI YACGTR 2 cut(s) 2396, 2654
BsaBI GATNNNNATC 1 cut(s) 1461
BsaHI GRCGYC 5 cut(s) 131, 327, 2441, 2605, 2719
BsaI GGTCTC 2 cut(s) 68, 2319
BsaJI CCNNGG 9 cut(s) 138, 346, 802, 1005, 1638, 1792, 2192, 2193, 2549
BsaWI WCCGGW 2 cut(s) 573, 2458
BsaXI ACNNNNNCTCC 4 cut(s) 233, 263, 2143, 2173
Bsc4I CCNNNNNNNGG 8 cut(s) 67, 313, 336, 580, 1918, 1919, 2140, 2555
Bse118I RCCGGY 2 cut(s) 127, 573
Bse8I GATNNNNATC 1 cut(s) 1461
BseBI CCWGG 2 cut(s) 987, 2194
BseDI CCNNGG 9 cut(s) 138, 346, 802, 1005, 1638, 1792, 2192, 2193, 2549
BseGI GGATG 4 cut(s) 730, 2119, 2305, 2457
BseJI GATNNNNATC 1 cut(s) 1461
BseLI CCNNNNNNNGG 8 cut(s) 67, 313, 336, 580, 1918, 1919, 2140, 2555
BseMII CTCAG 4 cut(s) 332, 1227, 1302, 2634
BseRI GAGGAG 4 cut(s) 257, 260, 772, 1254
BseSI GKGCMC 1 cut(s) 1014
BseX3I CGGCCG 1 cut(s) 168
BseXI GCAGC 8 cut(s) 338, 828, 1170, 1385, 1573, 1576, 1579, 2528
BseYI CCCAGC 3 cut(s) 197, 1912, 2435
BsgI GTGCAG 2 cut(s) 1311, 1392
Bsh1236I CGCG 2 cut(s) 298, 300
Bsh1285I CGRYCG 1 cut(s) 171
BshFI GGCC 6 cut(s) 71, 170, 266, 1875, 2276, 2686
BshNI GGYRCC 3 cut(s) 1009, 1908, 2455
BshTI ACCGGT 1 cut(s) 573
BsiEI CGRYCG 1 cut(s) 171
BsiHKAI GWGCWC 3 cut(s) 60, 101, 985
BsiHKCI CYCGRG 2 cut(s) 83, 2444
BsiSI CCGG 4 cut(s) 128, 356, 574, 2459
BslFI GGGAC 6 cut(s) 215, 1273, 1773, 2208, 2452, 2531
BslI CCNNNNNNNGG 8 cut(s) 67, 313, 336, 580, 1918, 1919, 2140, 2555
BsmAI GTCTC 6 cut(s) 68, 80, 1664, 2319, 2340, 2717
BsmBI CGTCTC 3 cut(s) 80, 1664, 2717
BsmFI GGGAC 6 cut(s) 215, 1273, 1773, 2208, 2452, 2531
BsmI GAATGC 1 cut(s) 2259
BsnI GGCC 6 cut(s) 71, 170, 266, 1875, 2276, 2686
Bso31I GGTCTC 2 cut(s) 68, 2319
BsoBI CYCGRG 2 cut(s) 83, 2444
Bsp119I TTCGAA 1 cut(s) 2423
Bsp1286I GDGCHC 4 cut(s) 60, 101, 985, 1014
Bsp1407I TGTACA 1 cut(s) 2502
Bsp19I CCATGG 1 cut(s) 2549
BspACI CCGC 5 cut(s) 117, 213, 330, 337, 2585
BspANI GGCC 6 cut(s) 71, 170, 266, 1875, 2276, 2686
BspCNI CTCAG 4 cut(s) 331, 1228, 1303, 2635
BspFNI CGCG 2 cut(s) 298, 300
BspHI TCATGA 1 cut(s) 1816
BspLI GGNNCC 7 cut(s) 288, 413, 585, 1011, 1910, 2457, 2631
BspMAI CTGCAG 1 cut(s) 1597
BspMI ACCTGC 2 cut(s) 2301, 2563
BspT104I TTCGAA 1 cut(s) 2423
BspT107I GGYRCC 3 cut(s) 1009, 1908, 2455
BspTNI GGTCTC 2 cut(s) 68, 2319
BsrBI CCGCTC 1 cut(s) 2585
BsrFI RCCGGY 2 cut(s) 127, 573
BsrGI TGTACA 1 cut(s) 2502
BssAI RCCGGY 2 cut(s) 127, 573
BssECI CCNNGG 9 cut(s) 138, 346, 802, 1005, 1638, 1792, 2192, 2193, 2549
BssNI GRCGYC 5 cut(s) 131, 327, 2441, 2605, 2719
BssT1I CCWWGG 3 cut(s) 802, 1792, 2549
Bst2UI CCWGG 2 cut(s) 987, 2194
Bst4CI ACNGT 6 cut(s) 206, 364, 1167, 1702, 2314, 2507
Bst6I CTCTTC 1 cut(s) 1692
BstACI GRCGYC 5 cut(s) 131, 327, 2441, 2605, 2719
BstAUI TGTACA 1 cut(s) 2502
BstBAI YACGTR 2 cut(s) 2396, 2654
BstBI TTCGAA 1 cut(s) 2423
BstC8I GCNNGC 3 cut(s) 129, 1022, 1098
BstDSI CCRYGG 3 cut(s) 138, 1638, 2549
BstF5I GGATG 4 cut(s) 730, 2119, 2305, 2457
BstFNI CGCG 2 cut(s) 298, 300
BstH2I RGCGCY 2 cut(s) 282, 842
BstHHI GCGC 4 cut(s) 281, 300, 841, 1974
BstMAI GTCTC 6 cut(s) 68, 80, 1664, 2319, 2340, 2717
BstMCI CGRYCG 1 cut(s) 171
BstMWI GCNNNNNNNGC 7 cut(s) 219, 278, 351, 411, 1325, 1592, 2671
BstNI CCWGG 2 cut(s) 987, 2194
BstNSI RCATGY 1 cut(s) 1409
BstSCI CCNGG 3 cut(s) 354, 985, 2192
BstSFI CTRYAG 1 cut(s) 1593
BstSLI GKGCMC 1 cut(s) 1014
BstUI CGCG 2 cut(s) 298, 300
BstV1I GCAGC 8 cut(s) 338, 828, 1170, 1385, 1573, 1576, 1579, 2528
BstV2I GAAGAC 3 cut(s) 791, 2412, 2714
BstX2I RGATCY 6 cut(s) 286, 517, 583, 1771, 1954, 2629
BstXI CCANNNNNNTGG 1 cut(s) 1511
BstYI RGATCY 6 cut(s) 286, 517, 583, 1771, 1954, 2629
BstZI CGGCCG 1 cut(s) 168
BsuRI GGCC 6 cut(s) 71, 170, 266, 1875, 2276, 2686
BtgI CCRYGG 3 cut(s) 138, 1638, 2549
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 4 cut(s) 730, 2119, 2305, 2457
BtsI GCAGTG 1 cut(s) 1602
BtsIMutI CAGTG 7 cut(s) 906, 1107, 1375, 1602, 1916, 2022, 2640
BveI ACCTGC 2 cut(s) 2301, 2563
Cac8I GCNNGC 3 cut(s) 129, 1022, 1098
CaiI CAGNNNCTG 1 cut(s) 1775
CciI TCATGA 1 cut(s) 1816
CfoI GCGC 4 cut(s) 281, 300, 841, 1974
Cfr10I RCCGGY 2 cut(s) 127, 573
Cfr13I GGNCC 3 cut(s) 256, 713, 2707
CseI GACGC 5 cut(s) 120, 335, 2449, 2533, 2594
Csp6I GTAC 7 cut(s) 763, 854, 912, 1881, 1909, 2397, 2503
CspAI ACCGGT 1 cut(s) 573
CviQI GTAC 7 cut(s) 763, 854, 912, 1881, 1909, 2397, 2503
DraI TTTAAA 1 cut(s) 2086
DriI GACNNNNNGTC 1 cut(s) 2332
EaeI YGGCCR 3 cut(s) 69, 168, 2274
EagI CGGCCG 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 1692
Eam1105I GACNNNNNGTC 1 cut(s) 2332
EarI CTCTTC 1 cut(s) 1692
EclXI CGGCCG 1 cut(s) 168
Eco130I CCWWGG 3 cut(s) 802, 1792, 2549
Eco31I GGTCTC 2 cut(s) 68, 2319
Eco47I GGWCC 3 cut(s) 256, 713, 2707
Eco47III AGCGCT 1 cut(s) 280
Eco52I CGGCCG 1 cut(s) 168
Eco57I CTGAAG 4 cut(s) 1122, 1943, 2046, 2351
Eco88I CYCGRG 2 cut(s) 83, 2444
EcoRI GAATTC 1 cut(s) 1801
EcoRII CCWGG 2 cut(s) 985, 2192
EcoT14I CCWWGG 3 cut(s) 802, 1792, 2549
ErhI CCWWGG 3 cut(s) 802, 1792, 2549
Esp3I CGTCTC 3 cut(s) 80, 1664, 2717
FaqI GGGAC 6 cut(s) 215, 1273, 1773, 2208, 2452, 2531
FauNDI CATATG 2 cut(s) 1183, 1990
FbaI TGATCA 1 cut(s) 1495
FblI GTMKAC 1 cut(s) 2429
Fnu4HI GCNGC 9 cut(s) 330, 352, 842, 1159, 1374, 1587, 1590, 1593, 2542
FokI GGATG 4 cut(s) 737, 2106, 2292, 2464
Fsp4HI GCNGC 9 cut(s) 330, 352, 842, 1159, 1374, 1587, 1590, 1593, 2542
FspBI CTAG 5 cut(s) 629, 1149, 1737, 1884, 2301
GlaI GCGC 4 cut(s) 280, 299, 840, 1973
GluI GCNGC 9 cut(s) 330, 352, 842, 1159, 1374, 1587, 1590, 1593, 2542
GsaI CCCAGC 3 cut(s) 201, 1916, 2439
GsuI CTGGAG 1 cut(s) 623
HaeII RGCGCY 2 cut(s) 282, 842
HaeIII GGCC 6 cut(s) 71, 170, 266, 1875, 2276, 2686
HapII CCGG 4 cut(s) 128, 356, 574, 2459
HgaI GACGC 5 cut(s) 120, 335, 2449, 2533, 2594
HhaI GCGC 4 cut(s) 281, 300, 841, 1974
Hin1I GRCGYC 5 cut(s) 131, 327, 2441, 2605, 2719
Hin6I GCGC 4 cut(s) 279, 298, 839, 1972
HinP1I GCGC 4 cut(s) 279, 298, 839, 1972
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HindIII AAGCTT 2 cut(s) 29, 1809
HpaI GTTAAC 1 cut(s) 571
HpaII CCGG 4 cut(s) 128, 356, 574, 2459
HphI GGTGA 5 cut(s) 193, 358, 1425, 1979, 2078
Hpy166II GTNNAC 7 cut(s) 138, 571, 931, 1642, 1881, 2430, 2503
Hpy8I GTNNAC 7 cut(s) 138, 571, 931, 1642, 1881, 2430, 2503
Hpy99I CGWCG 4 cut(s) 136, 305, 329, 2721
HpyAV CCTTC 5 cut(s) 394, 738, 1097, 2365, 2697
HpyCH4III ACNGT 6 cut(s) 206, 364, 1167, 1702, 2314, 2507
HpyCH4IV ACGT 4 cut(s) 462, 2395, 2653, 2719
HpyF10VI GCNNNNNNNGC 7 cut(s) 219, 278, 351, 411, 1325, 1592, 2671
HpySE526I ACGT 4 cut(s) 462, 2395, 2653, 2719
Hsp92I GRCGYC 5 cut(s) 131, 327, 2441, 2605, 2719
HspAI GCGC 4 cut(s) 279, 298, 839, 1972
KpnI GGTACC 1 cut(s) 1912
KroI GCCGGC 1 cut(s) 127
KroNI GCCGGC 1 cut(s) 129
Ksp22I TGATCA 1 cut(s) 1495
KspAI GTTAAC 1 cut(s) 571
LmnI GCTCC 5 cut(s) 411, 923, 988, 1050, 2582
Lsp1109I GCAGC 8 cut(s) 338, 828, 1170, 1385, 1573, 1576, 1579, 2528
LweI GCATC 8 cut(s) 492, 715, 1051, 1187, 1439, 2059, 2227, 2329
MaeI CTAG 5 cut(s) 629, 1149, 1737, 1884, 2301
MaeII ACGT 4 cut(s) 462, 2395, 2653, 2719
MaeIII GTNAC 7 cut(s) 364, 633, 768, 1895, 2149, 2378, 2391
MbiI CCGCTC 1 cut(s) 2585
MflI RGATCY 6 cut(s) 286, 517, 583, 1771, 1954, 2629
MhlI GDGCHC 4 cut(s) 60, 101, 985, 1014
MlsI TGGCCA 1 cut(s) 2276
MluNI TGGCCA 1 cut(s) 2276
MlyI GAGTC 4 cut(s) 42, 100, 641, 1255
MmeI TCCRAC 5 cut(s) 799, 1398, 2569, 2716, 2740
Mox20I TGGCCA 1 cut(s) 2276
MreI CGCCGGCG 1 cut(s) 127
MroNI GCCGGC 1 cut(s) 127
MroXI GAANNNNTTC 2 cut(s) 1801, 1812
MscI TGGCCA 1 cut(s) 2276
MseI TTAA 8 cut(s) 570, 662, 671, 849, 939, 2085, 2108, 2468
MslI CAYNNNNRTG 4 cut(s) 39, 1112, 1509, 2124
Msp20I TGGCCA 1 cut(s) 2276
MspI CCGG 4 cut(s) 128, 356, 574, 2459
MspR9I CCNGG 3 cut(s) 356, 987, 2194
Mva1269I GAATGC 1 cut(s) 2259
MvaI CCWGG 2 cut(s) 987, 2194
MvnI CGCG 2 cut(s) 298, 300
MwoI GCNNNNNNNGC 7 cut(s) 219, 278, 351, 411, 1325, 1592, 2671
NaeI GCCGGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 356
NcoI CCATGG 1 cut(s) 2549
NdeI CATATG 2 cut(s) 1183, 1990
NgoMIV GCCGGC 1 cut(s) 127
NlaIV GGNNCC 7 cut(s) 288, 413, 585, 1011, 1910, 2457, 2631
NmeAIII GCCGAG 3 cut(s) 97, 327, 1030
NmuCI GTSAC 5 cut(s) 364, 633, 1895, 2378, 2391
NspI RCATGY 1 cut(s) 1409
NspV TTCGAA 1 cut(s) 2423
OliI CACNNNNGTG 1 cut(s) 39
PagI TCATGA 1 cut(s) 1816
PasI CCCWGGG 1 cut(s) 2193
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PctI GAATGC 1 cut(s) 2259
PdiI GCCGGC 1 cut(s) 129
PdmI GAANNNNTTC 2 cut(s) 1801, 1812
PflMI CCANNNNNTGG 1 cut(s) 67
PfoI TCCNGGA 1 cut(s) 985
PinAI ACCGGT 1 cut(s) 573
PkrI GCNGC 9 cut(s) 331, 353, 843, 1160, 1375, 1588, 1591, 1594, 2543
PleI GAGTC 4 cut(s) 42, 99, 640, 1255
PpsI GAGTC 4 cut(s) 42, 99, 640, 1255
Ppu21I YACGTR 2 cut(s) 2396, 2654
PshBI ATTAAT 1 cut(s) 671
Psp6I CCWGG 2 cut(s) 985, 2192
PspFI CCCAGC 3 cut(s) 197, 1912, 2435
PspGI CCWGG 2 cut(s) 985, 2192
PspN4I GGNNCC 7 cut(s) 288, 413, 585, 1011, 1910, 2457, 2631
PspPI GGNCC 3 cut(s) 256, 713, 2707
PsrI GAACNNNNNNTAC 2 cut(s) 2734, 2766
PstI CTGCAG 1 cut(s) 1597
PstNI CAGNNNCTG 1 cut(s) 1775
PsuI RGATCY 6 cut(s) 286, 517, 583, 1771, 1954, 2629
RsaI GTAC 7 cut(s) 764, 855, 913, 1882, 1910, 2398, 2504
RsaNI GTAC 7 cut(s) 763, 854, 912, 1881, 1909, 2397, 2503
RseI CAYNNNNRTG 4 cut(s) 39, 1112, 1509, 2124
SaqAI TTAA 8 cut(s) 570, 662, 671, 849, 939, 2085, 2108, 2468
SatI GCNGC 9 cut(s) 330, 352, 842, 1159, 1374, 1587, 1590, 1593, 2542
Sau96I GGNCC 3 cut(s) 256, 713, 2707
SchI GAGTC 4 cut(s) 42, 100, 641, 1255
ScrFI CCNGG 3 cut(s) 356, 987, 2194
SduI GDGCHC 4 cut(s) 60, 101, 985, 1014
SfaNI GCATC 8 cut(s) 492, 715, 1051, 1187, 1439, 2059, 2227, 2329
SfcI CTRYAG 1 cut(s) 1593
SfuI TTCGAA 1 cut(s) 2423
SgrAI CRCCGGYG 1 cut(s) 127
SinI GGWCC 3 cut(s) 256, 713, 2707
SmiMI CAYNNNNRTG 4 cut(s) 39, 1112, 1509, 2124
SmlI CTYRAG 2 cut(s) 375, 1581
SmoI CTYRAG 2 cut(s) 375, 1581
SpeI ACTAGT 2 cut(s) 1736, 1883
SsiI CCGC 5 cut(s) 117, 213, 330, 337, 2585
SspMI CTAG 5 cut(s) 629, 1149, 1737, 1884, 2301
StyD4I CCNGG 3 cut(s) 354, 985, 2192
StyI CCWWGG 3 cut(s) 802, 1792, 2549
TaaI ACNGT 6 cut(s) 206, 364, 1167, 1702, 2314, 2507
TaiI ACGT 4 cut(s) 465, 2398, 2656, 2722
TaqI TCGA 7 cut(s) 5, 324, 387, 447, 779, 2323, 2423
TatI WGTACW 2 cut(s) 1880, 2502
TauI GCSGC 1 cut(s) 332
Tru1I TTAA 8 cut(s) 570, 662, 671, 849, 939, 2085, 2108, 2468
Tru9I TTAA 8 cut(s) 570, 662, 671, 849, 939, 2085, 2108, 2468
TscAI CASTG 7 cut(s) 913, 1114, 1375, 1602, 1923, 2029, 2640
TseFI GTSAC 5 cut(s) 364, 633, 1895, 2378, 2391
TseI GCWGC 8 cut(s) 351, 841, 1158, 1373, 1586, 1589, 1592, 2541
Tsp45I GTSAC 5 cut(s) 364, 633, 1895, 2378, 2391
TspGWI ACGGA 3 cut(s) 652, 787, 2699
TspRI CASTG 7 cut(s) 913, 1114, 1375, 1602, 1923, 2029, 2640
Van91I CCANNNNNTGG 1 cut(s) 67
VpaK11BI GGWCC 3 cut(s) 256, 713, 2707
VspI ATTAAT 1 cut(s) 671
XapI RAATTY 8 cut(s) 481, 735, 1030, 1388, 1801, 1941, 2042, 2613
XbaI TCTAGA 2 cut(s) 628, 1148
XceI RCATGY 1 cut(s) 1409
XmiI GTMKAC 1 cut(s) 2429
XmnI GAANNNNTTC 2 cut(s) 1801, 1812
XspI CTAG 5 cut(s) 629, 1149, 1737, 1884, 2301
ZraI GACGTC 1 cut(s) 2720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.