The glycine cleavage system catalyzes the degradation of glycine
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G26080 AT4G33010 AT4G33010 |
| fragaria_vesca | FvH4_2g31940 |
| malus_domestica | MD08G1061500.v1.1 MD15G1060200.v1.1 |
| prunus_persica | Prupe.1G412100_v2.0.a1 |
| pyrus_communis | pycom08g05100 pycom15g05650 pycom16g25720 |
| rosa_chinensis | RchiOBHm_Chr6g0310901 |
| rosa_laevigata | RLG00000010452 |
| rosa_multiflora | Rmu_ssc0000395.1_g000001 |
| rosa_roxburghii | Rroxscaffold_7G00158200 |
| rosa_rugosa | Rorug06G0383900 |
| rosa_samantha | Rh6BG507800 Rh6CG513800 |
| rosa_wichuraiana | Rw6G043350 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 164 |
| Acc65I | GGTACC | 1 cut(s) | 2489 |
| AccB1I | GGYRCC | 2 cut(s) | 2416, 2489 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 2440, 2795 |
| AccI | GTMKAC | 1 cut(s) | 2259 |
| AccII | CGCG | 1 cut(s) | 830 |
| AclI | AACGTT | 1 cut(s) | 2925 |
| AclWI | GGATC | 6 cut(s) | 840, 1728, 1749, 1762, 2007, 2521 |
| AcoI | YGGCCR | 5 cut(s) | 705, 1092, 1179, 2786, 2887 |
| AcsI | RAATTY | 2 cut(s) | 1545, 2457 |
| AcuI | CTGAAG | 5 cut(s) | 1140, 1314, 1450, 2150, 2951 |
| AfeI | AGCGCT | 2 cut(s) | 44, 2624 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 1937, 2369, 2387, 2440, 2453, 2795, 3050 |
| AflII | CTTAAG | 2 cut(s) | 815, 836 |
| AflIII | ACRYGT | 1 cut(s) | 3055 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2974 |
| AjiI | CACGTC | 1 cut(s) | 3056 |
| AjnI | CCWGG | 5 cut(s) | 576, 1089, 1123, 2380, 2794 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2396, 2428 |
| Alw21I | GWGCWC | 4 cut(s) | 13, 86, 441, 2941 |
| Alw26I | GTCTC | 4 cut(s) | 1573, 1628, 2481, 2961 |
| AlwI | GGATC | 6 cut(s) | 840, 1728, 1749, 1762, 2007, 2521 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 2795, 3110, 3137 |
| Ama87I | CYCGRG | 2 cut(s) | 2305, 2991 |
| Aor51HI | AGCGCT | 2 cut(s) | 44, 2624 |
| ApoI | RAATTY | 2 cut(s) | 1545, 2457 |
| Asp718I | GGTACC | 1 cut(s) | 2489 |
| AspLEI | GCGC | 4 cut(s) | 45, 165, 1171, 2625 |
| AspS9I | GGNCC | 7 cut(s) | 228, 1073, 1289, 1338, 2378, 2390, 2771 |
| AvaI | CYCGRG | 2 cut(s) | 2305, 2991 |
| AvaII | GGWCC | 6 cut(s) | 228, 1073, 1289, 2378, 2390, 2771 |
| BaeGI | GKGCMC | 1 cut(s) | 366 |
| BalI | TGGCCA | 5 cut(s) | 707, 1094, 1181, 2788, 2889 |
| BamHI | GGATCC | 1 cut(s) | 1754 |
| BanI | GGYRCC | 2 cut(s) | 2416, 2489 |
| BanII | GRGCYC | 2 cut(s) | 86, 2941 |
| BbsI | GAAGAC | 3 cut(s) | 96, 144, 746 |
| Bbv12I | GWGCWC | 4 cut(s) | 13, 86, 441, 2941 |
| BbvCI | CCTCAGC | 2 cut(s) | 1851, 2142 |
| BceAI | ACGGC | 1 cut(s) | 221 |
| BciT130I | CCWGG | 5 cut(s) | 578, 1091, 1125, 2382, 2796 |
| BciVI | GTATCC | 2 cut(s) | 897, 2193 |
| BclI | TGATCA | 2 cut(s) | 630, 1531 |
| BcoDI | GTCTC | 4 cut(s) | 1573, 1628, 2481, 2961 |
| BfaI | CTAG | 3 cut(s) | 191, 795, 2055 |
| BfmI | CTRYAG | 1 cut(s) | 1839 |
| BfoI | RGCGCY | 2 cut(s) | 46, 2626 |
| BfrI | CTTAAG | 2 cut(s) | 815, 836 |
| BfuI | GTATCC | 2 cut(s) | 897, 2193 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 242 |
| BglII | AGATCT | 1 cut(s) | 2223 |
| BlpI | GCTNAGC | 1 cut(s) | 315 |
| BmcAI | AGTACT | 1 cut(s) | 1473 |
| Bme18I | GGWCC | 6 cut(s) | 228, 1073, 1289, 2378, 2390, 2771 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2974 |
| BmeT110I | CYCGRG | 2 cut(s) | 2305, 2991 |
| BmgBI | CACGTC | 1 cut(s) | 3056 |
| BmgT120I | GGNCC | 7 cut(s) | 228, 1073, 1289, 1338, 2378, 2390, 2771 |
| BmrI | ACTGGG | 2 cut(s) | 556, 1914 |
| BmuI | ACTGGG | 2 cut(s) | 556, 1914 |
| BpiI | GAAGAC | 3 cut(s) | 96, 144, 746 |
| BpmI | CTGGAG | 1 cut(s) | 1968 |
| Bpu10I | CCTNAGC | 4 cut(s) | 1242, 1851, 2103, 2142 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 315 |
| BpuEI | CTTGAG | 4 cut(s) | 455, 623, 1086, 2716 |
| Bsa29I | ATCGAT | 3 cut(s) | 351, 371, 386 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1530 |
| BsaI | GGTCTC | 1 cut(s) | 1628 |
| BsaJI | CCNNGG | 8 cut(s) | 828, 890, 1518, 2305, 2507, 2565, 2653, 2991 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 2241, 2271, 2310, 2340 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 1937, 2369, 2387, 2440, 2453, 2795, 3050 |
| Bse1I | ACTGG | 8 cut(s) | 205, 562, 649, 1479, 1796, 1909, 2455, 2789 |
| Bse3DI | GCAATG | 5 cut(s) | 204, 231, 708, 1922, 2017 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1530 |
| BseBI | CCWGG | 5 cut(s) | 578, 1091, 1125, 2382, 2796 |
| BseCI | ATCGAT | 3 cut(s) | 351, 371, 386 |
| BseDI | CCNNGG | 8 cut(s) | 828, 890, 1518, 2305, 2507, 2565, 2653, 2991 |
| BseGI | GGATG | 6 cut(s) | 495, 1122, 1531, 1825, 2272, 2434 |
| BseJI | GATNNNNATC | 1 cut(s) | 1530 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 1937, 2369, 2387, 2440, 2453, 2795, 3050 |
| BseMI | GCAATG | 5 cut(s) | 204, 231, 708, 1922, 2017 |
| BseNI | ACTGG | 8 cut(s) | 205, 562, 649, 1479, 1796, 1909, 2455, 2789 |
| BseRI | GAGGAG | 1 cut(s) | 2931 |
| BseSI | GKGCMC | 1 cut(s) | 366 |
| BseYI | CCCAGC | 3 cut(s) | 97, 1334, 3010 |
| BsgI | GTGCAG | 4 cut(s) | 2019, 2487, 2502, 3076 |
| Bsh1236I | CGCG | 1 cut(s) | 830 |
| BshNI | GGYRCC | 2 cut(s) | 2416, 2489 |
| BshVI | ATCGAT | 3 cut(s) | 351, 371, 386 |
| BsiHKAI | GWGCWC | 4 cut(s) | 13, 86, 441, 2941 |
| BsiHKCI | CYCGRG | 2 cut(s) | 2305, 2991 |
| BsiSI | CCGG | 9 cut(s) | 28, 210, 226, 890, 1004, 1938, 2306, 2653, 2992 |
| BslFI | GGGAC | 4 cut(s) | 907, 1226, 1381, 2583 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 1937, 2369, 2387, 2440, 2453, 2795, 3050 |
| BsmAI | GTCTC | 4 cut(s) | 1573, 1628, 2481, 2961 |
| BsmFI | GGGAC | 4 cut(s) | 907, 1226, 1381, 2583 |
| BsmI | GAATGC | 5 cut(s) | 940, 1085, 2287, 2358, 2608 |
| Bso31I | GGTCTC | 1 cut(s) | 1628 |
| BsoBI | CYCGRG | 2 cut(s) | 2305, 2991 |
| Bsp1286I | GDGCHC | 5 cut(s) | 13, 86, 366, 441, 2941 |
| Bsp1407I | TGTACA | 2 cut(s) | 120, 1760 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 315 |
| BspDI | ATCGAT | 3 cut(s) | 351, 371, 386 |
| BspFNI | CGCG | 1 cut(s) | 830 |
| BspHI | TCATGA | 2 cut(s) | 2239, 2805 |
| BspPI | GGATC | 6 cut(s) | 840, 1728, 1749, 1762, 2007, 2521 |
| BspQI | GCTCTTC | 1 cut(s) | 2118 |
| BspT107I | GGYRCC | 2 cut(s) | 2416, 2489 |
| BspTI | CTTAAG | 2 cut(s) | 815, 836 |
| BspTNI | GGTCTC | 1 cut(s) | 1628 |
| BsrDI | GCAATG | 5 cut(s) | 204, 231, 708, 1922, 2017 |
| BsrGI | TGTACA | 2 cut(s) | 120, 1760 |
| BsrI | ACTGG | 8 cut(s) | 205, 562, 649, 1479, 1796, 1909, 2455, 2789 |
| BssECI | CCNNGG | 8 cut(s) | 828, 890, 1518, 2305, 2507, 2565, 2653, 2991 |
| BssNAI | GTATAC | 1 cut(s) | 2260 |
| BssT1I | CCWWGG | 3 cut(s) | 1518, 2507, 2565 |
| Bst1107I | GTATAC | 1 cut(s) | 2260 |
| Bst2UI | CCWGG | 5 cut(s) | 578, 1091, 1125, 2382, 2796 |
| Bst4CI | ACNGT | 9 cut(s) | 257, 361, 950, 1402, 1414, 1495, 1739, 2042, 2671 |
| Bst6I | CTCTTC | 3 cut(s) | 2118, 2874, 3102 |
| BstAFI | CTTAAG | 2 cut(s) | 815, 836 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 1233, 1589, 1687, 3137 |
| BstAUI | TGTACA | 2 cut(s) | 120, 1760 |
| BstC8I | GCNNGC | 5 cut(s) | 9, 271, 599, 1167, 1562 |
| BstDSI | CCRYGG | 1 cut(s) | 828 |
| BstEII | GGTNACC | 1 cut(s) | 3074 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 3048 |
| BstF5I | GGATG | 6 cut(s) | 495, 1122, 1531, 1825, 2272, 2434 |
| BstFNI | CGCG | 1 cut(s) | 830 |
| BstH2I | RGCGCY | 2 cut(s) | 46, 2626 |
| BstHHI | GCGC | 4 cut(s) | 45, 165, 1171, 2625 |
| BstMAI | GTCTC | 4 cut(s) | 1573, 1628, 2481, 2961 |
| BstNI | CCWGG | 5 cut(s) | 578, 1091, 1125, 2382, 2796 |
| BstNSI | RCATGY | 1 cut(s) | 1430 |
| BstPI | GGTNACC | 1 cut(s) | 3074 |
| BstSFI | CTRYAG | 1 cut(s) | 1839 |
| BstSLI | GKGCMC | 1 cut(s) | 366 |
| BstUI | CGCG | 1 cut(s) | 830 |
| BstV2I | GAAGAC | 3 cut(s) | 96, 144, 746 |
| BstX2I | RGATCY | 5 cut(s) | 832, 1720, 1754, 2223, 2513 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1803, 3018 |
| BstYI | RGATCY | 5 cut(s) | 832, 1720, 1754, 2223, 2513 |
| BstZ17I | GTATAC | 1 cut(s) | 2260 |
| Bsu15I | ATCGAT | 3 cut(s) | 351, 371, 386 |
| BsuI | GTATCC | 2 cut(s) | 897, 2193 |
| BsuTUI | ATCGAT | 3 cut(s) | 351, 371, 386 |
| BtgI | CCRYGG | 1 cut(s) | 828 |
| BtgZI | GCGATG | 1 cut(s) | 2871 |
| BtrI | CACGTC | 1 cut(s) | 3056 |
| BtsCI | GGATG | 6 cut(s) | 495, 1122, 1531, 1825, 2272, 2434 |
| BtsI | GCAGTG | 4 cut(s) | 1236, 2712, 2954, 3135 |
| Cac8I | GCNNGC | 5 cut(s) | 9, 271, 599, 1167, 1562 |
| CaiI | CAGNNNCTG | 3 cut(s) | 2795, 3110, 3137 |
| CciI | TCATGA | 2 cut(s) | 2239, 2805 |
| CfoI | GCGC | 4 cut(s) | 45, 165, 1171, 2625 |
| Cfr13I | GGNCC | 7 cut(s) | 228, 1073, 1289, 1338, 2378, 2390, 2771 |
| Cfr42I | CCGCGG | 1 cut(s) | 831 |
| Cfr9I | CCCGGG | 2 cut(s) | 2305, 2991 |
| ClaI | ATCGAT | 3 cut(s) | 351, 371, 386 |
| CseI | GACGC | 1 cut(s) | 2735 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2974 |
| EaeI | YGGCCR | 5 cut(s) | 705, 1092, 1179, 2786, 2887 |
| Eam1104I | CTCTTC | 3 cut(s) | 2118, 2874, 3102 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2974 |
| EarI | CTCTTC | 3 cut(s) | 2118, 2874, 3102 |
| EciI | GGCGGA | 2 cut(s) | 504, 1034 |
| Ecl136II | GAGCTC | 2 cut(s) | 84, 2939 |
| Eco130I | CCWWGG | 3 cut(s) | 1518, 2507, 2565 |
| Eco147I | AGGCCT | 2 cut(s) | 245, 1299 |
| Eco24I | GRGCYC | 2 cut(s) | 86, 2941 |
| Eco31I | GGTCTC | 1 cut(s) | 1628 |
| Eco47I | GGWCC | 6 cut(s) | 228, 1073, 1289, 2378, 2390, 2771 |
| Eco47III | AGCGCT | 2 cut(s) | 44, 2624 |
| Eco53kI | GAGCTC | 2 cut(s) | 84, 2939 |
| Eco57I | CTGAAG | 5 cut(s) | 1140, 1314, 1450, 2150, 2951 |
| Eco88I | CYCGRG | 2 cut(s) | 2305, 2991 |
| Eco91I | GGTNACC | 1 cut(s) | 3074 |
| EcoICRI | GAGCTC | 2 cut(s) | 84, 2939 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 3048 |
| EcoO109I | RGGNCCY | 3 cut(s) | 1073, 1338, 2390 |
| EcoO65I | GGTNACC | 1 cut(s) | 3074 |
| EcoRI | GAATTC | 1 cut(s) | 2457 |
| EcoRII | CCWGG | 5 cut(s) | 576, 1089, 1123, 2380, 2794 |
| EcoT14I | CCWWGG | 3 cut(s) | 1518, 2507, 2565 |
| EcoT22I | ATGCAT | 1 cut(s) | 2584 |
| EcoT38I | GRGCYC | 2 cut(s) | 86, 2941 |
| ErhI | CCWWGG | 3 cut(s) | 1518, 2507, 2565 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2396, 2428 |
| FaqI | GGGAC | 4 cut(s) | 907, 1226, 1381, 2583 |
| FauI | CCCGC | 1 cut(s) | 278 |
| FbaI | TGATCA | 2 cut(s) | 630, 1531 |
| FblI | GTMKAC | 1 cut(s) | 2259 |
| FokI | GGATG | 6 cut(s) | 502, 1129, 1538, 1812, 2279, 2421 |
| FriOI | GRGCYC | 2 cut(s) | 86, 2941 |
| FspAI | RTGCGCAY | 1 cut(s) | 164 |
| FspBI | CTAG | 3 cut(s) | 191, 795, 2055 |
| FspI | TGCGCA | 1 cut(s) | 164 |
| GlaI | GCGC | 4 cut(s) | 44, 164, 1170, 2624 |
| GsaI | CCCAGC | 3 cut(s) | 101, 1338, 3014 |
| GsuI | CTGGAG | 1 cut(s) | 1968 |
| HaeII | RGCGCY | 2 cut(s) | 46, 2626 |
| HapII | CCGG | 9 cut(s) | 28, 210, 226, 890, 1004, 1938, 2306, 2653, 2992 |
| HgaI | GACGC | 1 cut(s) | 2735 |
| HhaI | GCGC | 4 cut(s) | 45, 165, 1171, 2625 |
| Hin6I | GCGC | 4 cut(s) | 43, 163, 1169, 2623 |
| HinP1I | GCGC | 4 cut(s) | 43, 163, 1169, 2623 |
| HincII | GTYRAC | 3 cut(s) | 417, 2692, 3061 |
| HindII | GTYRAC | 3 cut(s) | 417, 2692, 3061 |
| HpaII | CCGG | 9 cut(s) | 28, 210, 226, 890, 1004, 1938, 2306, 2653, 2992 |
| Hpy166II | GTNNAC | 7 cut(s) | 123, 417, 558, 567, 2260, 2692, 3061 |
| Hpy8I | GTNNAC | 7 cut(s) | 123, 417, 558, 567, 2260, 2692, 3061 |
| HpyCH4III | ACNGT | 9 cut(s) | 257, 361, 950, 1402, 1414, 1495, 1739, 2042, 2671 |
| HpyCH4IV | ACGT | 5 cut(s) | 138, 864, 2180, 2925, 3055 |
| HpySE526I | ACGT | 5 cut(s) | 138, 864, 2180, 2925, 3055 |
| HspAI | GCGC | 4 cut(s) | 43, 163, 1169, 2623 |
| KpnI | GGTACC | 1 cut(s) | 2493 |
| Ksp22I | TGATCA | 2 cut(s) | 630, 1531 |
| KspI | CCGCGG | 1 cut(s) | 831 |
| LguI | GCTCTTC | 1 cut(s) | 2118 |
| LmnI | GCTCC | 9 cut(s) | 4, 89, 1195, 1940, 2270, 2318, 2936, 2944, 3027 |
| MaeI | CTAG | 3 cut(s) | 191, 795, 2055 |
| MaeII | ACGT | 5 cut(s) | 138, 864, 2180, 2925, 3055 |
| MfeI | CAATTG | 2 cut(s) | 760, 1431 |
| MflI | RGATCY | 5 cut(s) | 832, 1720, 1754, 2223, 2513 |
| MhlI | GDGCHC | 5 cut(s) | 13, 86, 366, 441, 2941 |
| MlsI | TGGCCA | 5 cut(s) | 707, 1094, 1181, 2788, 2889 |
| MluNI | TGGCCA | 5 cut(s) | 707, 1094, 1181, 2788, 2889 |
| MlyI | GAGTC | 4 cut(s) | 614, 1472, 1907, 2565 |
| MmeI | TCCRAC | 4 cut(s) | 891, 1330, 2291, 2823 |
| Mox20I | TGGCCA | 5 cut(s) | 707, 1094, 1181, 2788, 2889 |
| Mph1103I | ATGCAT | 1 cut(s) | 2584 |
| MscI | TGGCCA | 5 cut(s) | 707, 1094, 1181, 2788, 2889 |
| MslI | CAYNNNNRTG | 3 cut(s) | 2010, 2244, 2804 |
| Msp20I | TGGCCA | 5 cut(s) | 707, 1094, 1181, 2788, 2889 |
| MspA1I | CMGCKG | 3 cut(s) | 692, 830, 2501 |
| MspCI | CTTAAG | 2 cut(s) | 815, 836 |
| MspI | CCGG | 9 cut(s) | 28, 210, 226, 890, 1004, 1938, 2306, 2653, 2992 |
| MunI | CAATTG | 2 cut(s) | 760, 1431 |
| Mva1269I | GAATGC | 5 cut(s) | 940, 1085, 2287, 2358, 2608 |
| MvaI | CCWGG | 5 cut(s) | 578, 1091, 1125, 2382, 2796 |
| MvnI | CGCG | 1 cut(s) | 830 |
| NmuCI | GTSAC | 9 cut(s) | 213, 257, 764, 823, 860, 1798, 2083, 2176, 3074 |
| NsbI | TGCGCA | 1 cut(s) | 164 |
| NsiI | ATGCAT | 1 cut(s) | 2584 |
| NspI | RCATGY | 1 cut(s) | 1430 |
| PagI | TCATGA | 2 cut(s) | 2239, 2805 |
| PceI | AGGCCT | 2 cut(s) | 245, 1299 |
| PciSI | GCTCTTC | 1 cut(s) | 2118 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 357 |
| PctI | GAATGC | 5 cut(s) | 940, 1085, 2287, 2358, 2608 |
| PfeI | GAWTC | 7 cut(s) | 182, 341, 373, 383, 544, 1151, 1744 |
| PflFI | GACNNNGTC | 2 cut(s) | 226, 1400 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 2440, 2795 |
| PleI | GAGTC | 4 cut(s) | 613, 1472, 1907, 2565 |
| PpsI | GAGTC | 4 cut(s) | 613, 1472, 1907, 2565 |
| PpuMI | RGGWCCY | 2 cut(s) | 1073, 2390 |
| Psp124BI | GAGCTC | 2 cut(s) | 86, 2941 |
| Psp1406I | AACGTT | 1 cut(s) | 2925 |
| Psp5II | RGGWCCY | 2 cut(s) | 1073, 2390 |
| Psp6I | CCWGG | 5 cut(s) | 576, 1089, 1123, 2380, 2794 |
| PspEI | GGTNACC | 1 cut(s) | 3074 |
| PspFI | CCCAGC | 3 cut(s) | 97, 1334, 3010 |
| PspGI | CCWGG | 5 cut(s) | 576, 1089, 1123, 2380, 2794 |
| PspPI | GGNCC | 7 cut(s) | 228, 1073, 1289, 1338, 2378, 2390, 2771 |
| PspPPI | RGGWCCY | 2 cut(s) | 1073, 2390 |
| PstNI | CAGNNNCTG | 3 cut(s) | 2795, 3110, 3137 |
| PsuI | RGATCY | 5 cut(s) | 832, 1720, 1754, 2223, 2513 |
| PsyI | GACNNNGTC | 2 cut(s) | 226, 1400 |
| PvuII | CAGCTG | 1 cut(s) | 692 |
| RseI | CAYNNNNRTG | 3 cut(s) | 2010, 2244, 2804 |
| SacI | GAGCTC | 2 cut(s) | 86, 2941 |
| SacII | CCGCGG | 1 cut(s) | 831 |
| SapI | GCTCTTC | 1 cut(s) | 2118 |
| Sau96I | GGNCC | 7 cut(s) | 228, 1073, 1289, 1338, 2378, 2390, 2771 |
| ScaI | AGTACT | 1 cut(s) | 1473 |
| SchI | GAGTC | 4 cut(s) | 614, 1472, 1907, 2565 |
| SduI | GDGCHC | 5 cut(s) | 13, 86, 366, 441, 2941 |
| SfcI | CTRYAG | 1 cut(s) | 1839 |
| Sfr303I | CCGCGG | 1 cut(s) | 831 |
| SgrBI | CCGCGG | 1 cut(s) | 831 |
| SinI | GGWCC | 6 cut(s) | 228, 1073, 1289, 2378, 2390, 2771 |
| SmaI | CCCGGG | 2 cut(s) | 2307, 2993 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 2010, 2244, 2804 |
| SmlI | CTYRAG | 6 cut(s) | 434, 602, 815, 836, 1101, 2695 |
| SmoI | CTYRAG | 6 cut(s) | 434, 602, 815, 836, 1101, 2695 |
| SseBI | AGGCCT | 2 cut(s) | 245, 1299 |
| SspI | AATATT | 1 cut(s) | 721 |
| SspMI | CTAG | 3 cut(s) | 191, 795, 2055 |
| SstI | GAGCTC | 2 cut(s) | 86, 2941 |
| StuI | AGGCCT | 2 cut(s) | 245, 1299 |
| StyI | CCWWGG | 3 cut(s) | 1518, 2507, 2565 |
| TaaI | ACNGT | 9 cut(s) | 257, 361, 950, 1402, 1414, 1495, 1739, 2042, 2671 |
| TaiI | ACGT | 5 cut(s) | 141, 867, 2183, 2928, 3058 |
| TaqI | TCGA | 7 cut(s) | 339, 351, 371, 386, 405, 810, 2895 |
| TatI | WGTACW | 5 cut(s) | 120, 564, 1107, 1471, 1760 |
| TauI | GCSGC | 2 cut(s) | 276, 1003 |
| TfiI | GAWTC | 7 cut(s) | 182, 341, 373, 383, 544, 1151, 1744 |
| TseFI | GTSAC | 9 cut(s) | 213, 257, 764, 823, 860, 1798, 2083, 2176, 3074 |
| Tsp45I | GTSAC | 9 cut(s) | 213, 257, 764, 823, 860, 1798, 2083, 2176, 3074 |
| TspGWI | ACGGA | 5 cut(s) | 561, 1385, 2382, 2775, 3014 |
| TspMI | CCCGGG | 2 cut(s) | 2305, 2991 |
| Tth111I | GACNNNGTC | 2 cut(s) | 226, 1400 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 2440, 2795 |
| Vha464I | CTTAAG | 2 cut(s) | 815, 836 |
| VpaK11BI | GGWCC | 6 cut(s) | 228, 1073, 1289, 2378, 2390, 2771 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 3048 |
| XapI | RAATTY | 2 cut(s) | 1545, 2457 |
| XceI | RCATGY | 1 cut(s) | 1430 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 1322, 2749, 2781 |
| XmaI | CCCGGG | 2 cut(s) | 2305, 2991 |
| XmiI | GTMKAC | 1 cut(s) | 2259 |
| XspI | CTAG | 3 cut(s) | 191, 795, 2055 |
| ZrmI | AGTACT | 1 cut(s) | 1473 |
| Zsp2I | ATGCAT | 1 cut(s) | 2584 |